Olympus CK40-RFA Operating instructions

REFLECTED LIGHT
FLUORESCENCE ATTACHMENT
CK40-RFA
REPAIR MANUAL
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OLYrvjPUS
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CK40-RFA
cm‘ CONTENTS
A. OUTLINE OF PRODUCT
1. Outline ......................................................................................................................... A-l
2. Features ....................................................................................................................... A-l
3. Using conditions ........................................................................................................... A-l
4. Specifications ............................................................................................................... A-2
5. Dimensions................................................................................................................... A-3
B. INSPECTION STANDARD
f 1. Inspection items andmethods ....................................................................................... B-l
C. DISASSEMBLY AND ASSEMBLY PROCEDURES
1. Slider unit ..................................................................................................................... C-l
2. Cube dovetail/diaphragm unit ....................................................................................... c-3
D. REPAIR PROCEDURE
1. Adjustment procedure................................................................................................... D-l
2. Adjusting the cubeworking force ................................................................................. D-2
3. Adjusting the click removing force of the cube
.............................................................. D-2
4. Adjusting the click removing force of the slider............................................................. D-3
5. Adjusting the optical axis.............................................................................................. D-4
6. Checking the FS image center....................................................................................... D-7
r E. JIGS AND TOOLS
1. List of jigs and tools ..................................................................................................... E-l
F. LUBRICANTS AND CHEMICALS
1. List of lubricants........................................................................................................... F-l
2. List of chemicals
........................................................................................................... F-l
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CK40-RFA A. OUTLINE OF PRODUCT
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2.
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3.
P
Outline
(1) This product is anilluminator of a reflected fluorescence systemdesignedfor CK40.
(2) Target market and users
Target market: Tissueculture routine market representedby culture, growth, immunity and
pharmacy
(3) Applicable microscope: CK40
Features
(1) Switching between B excitation and G excitation is easybecausethe 2-cube IN-OUT
method is adopted.
(2) Two-wavelength observation and transmitted light observation canbe set simultaneously.
Using conditions
(1) This illuminator is designedfor B excitation and G excitation observation only. (This is
(2) becausethe lamp housing (U-LHSOHG) hasa built-in ultraviolet protective filter.) Use a shutter for transmitted light observation.
(3) Be sure to mount the shieldcovers to the RFA basefrom both sidesof cube. This prevents
flashlight.
(4) Weak flashlight goesinto the visual field at the time of cube switching. However, this
(5) involves no hazardbecausethe lamp housing hasabuilt-in ultraviolet protective filter. The standard performance is not guaranteedif it is combined with a lamp housing other
than U-LHSOHG.
(6) The standard performance is not guaranteedif anobjective other than the designatedtypes
is used. (Refer to Item 11 in “4. Specifications.“)
A-l

CK40-RFA A. OUTLINE OF PRODUCT
4. Specifications
Item Specitications Remarks
1 Weight 1.14kg CK40-RFA single
unit
2 Fieldnumber
3 Projection
magnification
4 Diaphragm
5 Observationmethod
F.N. 20
3.139x
Built-in field diaphragm(with centeringmechanism) $2-$5mm
1)Reflectedlight fluorescence(B excitation,G Thelamphousing
excitation) (U-LHSOHG)hasa
2)Transmittedlightobservation built-in ultraviolet
protectivefilter
(L-42).
6 Observationsxtitching 1)Movabledovetailtype(with opticalpathclick)
method 2)Maximumof 2 cubes
7 Shutter Slidetype(with aclick at thecenterandstopperson
thebothends)
8 Filter With afilter pocket 32ND25
9 Reflectedlight 1)Supportedexcitation:B excitation,G excitation
fluorescence 2) Cubetype:Built-in dichroicmirror, excitation
filter andbarrierfilter
10 Cube
3)Auxiliary excitationfilter: None
4)Auxiliary barrierfilter: None
1)Cubetype
CX-DMB-2 for B excitation
CX-DMG-2 for G excitation
2)Thecubefor fluorescencehasbuilt-in fixed
dichroicmirror, excitationfilter, andbarrier filter.
11 Objective SPlan4XPL-6
DAchlOXPL-6
LWDCDAch20XPL-6
LWDCDPlan40X
Designatedobjective
A-2

A. OUTLINE OF PRODUCT
CK40-RFA
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5. Dimensions
Unit: mm
A-3

CK40-RFA B. INSPECTION STANDARD
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1. Inspection items and methods
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Part Item Standard Method
lluminator Cube working 250 t 50 g Insert the cube with no click pressure applied to it
force (2450 mN 2 490 n-8) and measurethe working force using a tension gauge.
Click removing 750 + 50 g Insert the cube with click pressure applied to it and
force of cube (7350 InN t 490 mN) measure the click removing force using a tension
gauge.
Click removing 500 + 100 g Insert the slider and measure the click removing force
force of slider (4900 mN 2 980 InN) using a tension gauge.
Optical axis 0.3 mm or less Combining the standard eyepiece (KNO048; with
adapter-l), a microscope stand (product), the
standard objective (KNOO15), and the cube (BH2-
UBF), read the displacement between the specimen
center and the visual field center in the right sleeve
using the reticle scale (1 graduation=O.l mm) of the
KNO048 (with adapter-l).
FS image It can be set at the center Combining the NCWHKlOX, a microscope stand
center of the visual field. (product), the cube (product: CX-DMB-2, CX-
DMG-2), U-LHSOHG (product), and U-RFLTSO
(product), focus on a name card (coloured with a
fluorescent pen) used as a specimen and make sure
that the FS image center can be set at the center of
the visual field.
B-l

CK40-RFA C. DISASSEMBLY AND ASSEMBLY PROCEDURES
1. Slider unit
0
-Q
C-l

CK40-RFA C. DISASSEMBLY AND ASSEMBLY PROCEDURES
pply adhesiveto the screw
c-2

CK40-RFA C. DISASSEMBLY AND ASSEMBLY PROCEDURES
2. Cube dovetail/diaphragm unit
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*1
c-3

CK40-RFA C. DISASSEMBLY AND ASSEMBLY PROCEDURES
No. Parts name
@ COVER
Screw Grease Adhesive Remarks
0 SCREW OT1131 Apply adhesiveto the screw.
Refer to “3. Adjusting the click
removing force of the cube (D-2).”
0 SPRING B3UO (*l)
@ SCREW (4 PCS.) OT1131 Apply adhesiveto the screws.
Refer to “2. Adjusting the cube
working force (D-2).”
@ SPRING (4 PCS.)
@ COVER PSK2X3SA (*2) 6 PCS.
0 DOVETAIL (2 PCS.)
@ PLATE (2 PCS.)
@ DOVETAIL AB3X12SA (*3) 2 PCS. OT1595 OT1131 Apply adhesiveto the screws (*3).
Apply greaseto the dovetail surface
only.
Refer to “5. Adjusting the optical
axis @-4).”
@ BASE
@ FIXING SPRING OT2008 Apply greaseto the reverse side.
@ LEVER-l OT1983 Apply adhesiveto the screw thread.
@ SPRING HOLDER OT1983 Apply adhesiveto the screw thread.
8 SPRING
@ SPINDLE
8 KNOB (2 PCS.) OT2008 Apply adhesiveto the screw thread.
@ FSFRAME ANU3X4SA (*4) 3 PCS. OT1131 Apply adhesiveto the screws (*4).
Refer to “6. Checking the FS image
center (D-7).”
@ RING
@ INNERFRAME
8 DIAPHRAGM
BLADE-A (8 PCS.)
@ OUTERFRAME
@ SPRING AHU4X4SA (*5) Refer to “4. Adjusting the click
B3UO (*6) removing force of the slider (D-3).”
@ SLIDER HOLDER-2 ABSX12SA (*7) 2 PCS.
c-4

CK40-RFA D. REPAIR PROCEDURE
1. Adjustment procedure
2. Adjusting the cube working force
(Refer to D-2.)
3. Adjusting the click removing force
of the cube (Refer to D-2.)
1
4. Adjusting the click removing force
Ofthe ‘lider I (Refer to D-3.) 1
5. Adjusting the optical axis
(Refer to D-4.)
6. Checking the FS image center
(Refer to D-7.)
D-l

CK40-RFA D. REPAIR PROCEDURE
2. Adjusting the cube working force
2-1 Set the cube shown in the following illustration to adjust the working force using atension
gauge.
Insertasetof SPRING@andSCREW0 at four
locations,fit theCX-DMB-2 0 (product)onthe
DOVETAIL 0, andmeasuretheworkingforcein
thewholestrokeusingaTENSION GAUGE 0.
Settingtheendof thetensiongaugeonthe(B, G)
knobposition,measuretheworking force.
Standardworkingforce: 2502 50g
(2450
If theresultisnotwithin thestandardrange,
adjustit by tighteningor looseningthefour pieces
of SCREW0.
3. Adjusting the click removing force of the cube
3-1 Set the cube shown in the following illustration to adjust the click removing force using a
tension gauge.
Insertasetof BALL @,SPRING 0 andSCREW
@>,fit theCX-DMB-2 0 (product)onthe
DOVETAIL 0, moveit to theclickingposition,
andmeasuretheclick removingforceusinga
TENSION GAUGE 0.
Settingtheendof thetensiongaugeonthe(B, G)
knobposition,measuretheremovingforce.
Standardremovingforce:750 -c50g
(7350mN 2 490mN)
If theresultisnotwithin thestandardrange,
adjustit by tighteningor looseningtheSCREW
63,.
D-2

CK40-RFA D. REPAIR PROCEDURE
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4. Adjusting the click removing force of the slider
4-1 Set the slider shown in the following illustration to adjust the click removing force using a
tension gauge.
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f-
Slider @
gauge0
Insert a set of BALL @, SPRING 0 andSCREW @, move the SLIDER 0 to the clicking
position of the SLIDER HOLDER-l 0, andmeasurethe click removing force using a TENSION
GAUGE 0.
Setting the end of the tension gauge at the center of the knob, measurethe removing force.
(“ Standard removing force: 500 + 100 g
(4900 mN + 980 mN)
If the result is not within the standard range, adjust it by tightening or loosening the SCREW 63.
D-3

CK40-RFA D. REPAIR PROCEDURE
5. Adjusting the optical axis
5-l Perform the adjustment in the following procedure.
(1) Remove the AB3X20SA screws(“1) from the LH MOUNT 0 of CKLCO-RFA0. Instead
of the LH MOUNT 0, mount the BXKC002 (optical alignmentjig) and RFA unit on
CK40 MAIN BODY 0 with AB3X25SA.
(2)
Insert the CUBE (CX-DMB-2) 0 into the dovetail of CK40-RFA 0 and mount the
COVER ASS’Y L/R 0 (The cube should be inserted in such away that the characterson
the CX-DMB-2 are seenin the reverse). / a
D-4

CK40-RFA D. REPAIR PROCEDURE
(3) Mount the KNOB ASS’Y-B on the cube through the COVER-R andmove the cube to the
optical path.
5-2 Setting thejigs shown below, adjust the optical axis.
Centering telescop
(KN0029)
Optical alignment jig
for illumination part
(BXKC002)
Standard objective
for optical axis
(KN0015)
5-3 Turn on the power of CK40 main body and observe the optical axisposition using the
centering telescope.
D-5

CK40-RFA D. REPAIR PROCEDURE
5-4 Loosen two SCREWS 0 andmove the CUBE DOVETAIL 0 to adjust the optical axis.
Standard: Within 3 graduationson reticle scaleof centering telescope(KNOO29)
If the result is not within the standardrange, return to “Adjusting the optical axis” on page D-
4 and repeat the work.
D-6

CK40-RFA D. REPAIR PROCEDURE
6. Checking the FS image center
6-1 Set the following jig and products to check the FSimagecenter:
l Eyepiece ............ NCWHKlOX
l Cube .................. CX-DMB-2 or CX-DMG-2 (product)
l Standard tube .... BKN0003 (Use it when no observation tube is available.)
l Reflected light fluorescenceunit ............. U-LHSOHG, U-RFLTSO (product)
l Other ................. Name card (coloured with afluorescent pen)
D-7

CK40-RFA D. REPAIR PROCEDURE
(
6-2 Mount the CK40-RFA on the CK40 main body, turn off the power of main body, set the
microscope to reflected fluorescenceobservation, andmove the two KNOBS 0 to make sure
that the FS image center canbe set at the center of the visual field.
D-8

CK40-RFA E. JIGS AND TOOLS
1. List of jigs and tools
No.
CX-DMB-2 (product) Cube
Description Page
D-2 - 8
or CX-DMG-2 (product)
OT1144
OT1145
KNO029
BXKC002
KNOOlS
U-LHSOHG(product)
Tensiongauge(500g)
Opticalalignmentjig for illuminationpart
Tensiongauge(1 kg)
Centeringtelescope
D-2
D-2 - 3
D-4 - 6
1D-4 - 6
LB standardobjectivefor opticalaxis D-4 - 6
Lamphousing D-7 - 8
a
I
U-RFLT.50(product) Powersupplyunit 1D-7 - 8
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E-l

CK40-RFA F. LUBRICANTS AND CHEMICALS
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1. List of lubricants
<Grease>
No. Description
OT1595 Siliconegrease
OT2008 Grease(medium) c-2, 4
c-4
Page
2. List of chemicals
p <Adhesive>
No.
OT1131
OT1983
Description
Shellac
Solvent-basedadhesive(red) c-2, 4 c-4
Page
F-l