Omegawave OMEGAZONE STD User manual

210621
Laser Speckle Blood Flow Imager
OMEGAZONE
STD
USER’S MANUAL
( LSI : ver 6.1, LIA : ver 6.0)

1
TABLE OF CONTENTS
Page
■ CAUTION 2
■ SUMMARY 3
■ HARDWARE 4
1. Parts name and the function
2. Setup of the stand
4. Lens adjustment
5. Polarization adjustment
6. Block diagram
■ LSI software 14
1. Controls and display
2. Project Configuration File
3. LSI Front Panel
4. LSI operation
■ LIA software 26
1. Controls and display
2. Analysis Configuration File
4. LIA Front Panel
5. LIA operation
■ Handling in use 40
■ Appendix 42
1. Lens, WD and measurement area(Working Distance)
2. Time for saving data and capacity
3. User name and organization change
4. License certification of OS
■ Specification 44
■ Warranty 46

2
CAUTION
1. Do not turn on the power switch of the power supply for the laser
unit and CMOS camera before connecting the cables of the laser unit
and the CMOS camera.
2. Check if there is enough capacity in the HDD for storing data
before measuring. Data cannot be stored if there is not enough
capacity.
3. Do not touch the lens, the optical filter and the CMOS camera by
bare hands.
4. Do not see the laser light through a lens or an optical instrument.
5. Do not update Windows and the other software automatically or
manually.
The function may interrupt the software of this instrument.
6. The function of FireWall is set as “OFF” because the interface of
the CMOS camera is Ethernet. Do not change the status.
7. Do not use this instrument as a conventional computer.
＿＿＿＿＿＿＿＿＿＿＿＿＿＿
This manual is changed without a preliminary notice.
There is a copyright of this manual in OMEGAWAVE, INC.
A reproduction without permission is prohibited in any kind of form.
Microsoft and Windows are registered trademarks of Microsoft Corporation.
Intel, Core i5 and Core i7 are registered trademarks of Intel Corporation.
OMEGAWAVE, INC. has the copyright of LSI and LIA software.

3
SUMMARY
The laser speckle blood flow imager, OMEGAZONE STD, is the instrument to
obtain the colored blood flow image of living tissue non-invasively and
continuously.
OMEGAZONE STD shows more than 2 images/sec with high resolution
(639-480), or 15 images/sec with low resolution (212 - 160).
Every point is captured by the pixel of the CMOS camera simultaneously,
therefore, there is no time lug between the all points and the each blood flow
value can be compared.
This OMEGAZONE STD consists of the laser unit, the CMOS camera, the lens
+ hybrid filter, the computer-based image processor and the stand.
The lens can be changed to adapt the measurement size. The measurement
size is about from 1 cm x 1 cm to 40 cm x 40 cm, and it is suitable for cerebral
blood flow measurement and limb blood flow measurement for small animals,
and for human.
OMEGAZONE STD includes two kinds of software. One is for measurement,
LSI, and the other is for analysis, LIA. Separating the software makes the
operation easier.
This instrument was developed based on the study of Dr. Kevin Forrester in
University of Calgary.

4
6. USB key
HARDWARE
1. Parts name and the function
1) Computer-based imager processor
This computer-based imager processor shows black/white real images by laser
light and tissue blood flow images calculated by the installed software. The
images captured by the CMOS camera are processed to obtain the blood flow
images. The operating system (the OS) is Windows 10.
The hard disk drive of this computer-based image processor is assigned to two
regions, C and E. The OS, the blood flow software and other application
software are installed in the C drive. The E drive is for data. Initial setting assigns
the E drive for saving.
Turn the power switch (I) on the keyboard after all cables were connected.
1. Computer-based
Image Processor
4. Laser Unit
3. CMOS camera
+ Lens
2. Power supply
5. Arm Stand
6. USB key

5
2) Power supply
The power supply contains the laser drive circuit, the power supply for the
CMOS camera and the intermediate connector for the CMOS camera.
Turn on the laser switch after the laser unit and the CMOS camera were
connected. Connection after the power being ON may destroy the laser diodes.
Front Rear
The main power switch is the key switch. Turn it to the clockwise direction for
the power ON. When the key is on the OFF side, the key can be removed.
The Laser SW is for ON/OFF of the laser light. The Red laser SW is for
ON/OFF of the red pointer laser light. The red laser light does not affect the
blood flow value.
Connect the 12-pin cable between the Laser unit and the Connector for laser
unit. Connect the 12-pin cable between the CMOS camera and the Connector
for CMOS camera, too.
The GigE connectors have IN and OUT. Connect one GigE cable from the
CMOS camera to the IN, and connect the other cable from the OUT to the
computer-based image processor. The IN and OUT is connected inside the
power supply, and the CMOS camera is not damaged by misconnection.
Red laser SW
Connector for
laser unit
GigE connectors for CMOS
camera and the computer
Connector for CMOS camera
Power connector
Laser SW
Main power SW

6
3) CMOS camera and Lens
The images are captured with the CMOS
camera.
The hybrid filter is attached on the lens.
This is the specialized filter combined of the
polarizer and the color filter not to detect the
direct reflection from tissue surface and the
light from outside.
The filter has the direction of polarization,
and it is shown as the white dot.
Make the white dot showing the same
direction of the dot of the laser unit.
Mono-focal Lens
Zoom Lens
The measuring area matrix of the W.D. and the lens is shown in Appendix.
Zoom Lens
Mono-focal Lens
CMOS camera
・
Hybrid filter
Hybrid filter
・
・
White dot
White dot

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4) Laser unit
The laser diodes for measurement (infrared, 780 nm) and the positioning (red,
650 nm) are installed in the laser unit. The left side is the laser for measurement.
The positioning laser can be turned off with the RL switch on the rear panel
when it is not needed.
The laser diodes have polarization,
and the direction is marked by the
white dot on the laser unit.
Turn the hybrid filter attached to the
lens to make the white dot show the
same direction as the white dot on the
laser unit.
※ Shut the shutter when the
measurement is over to not to
collect dust on the lens inside.
5) Arm stand
The arm stand holds the CMOS camera, the lens and the laser unit.
Adjust the working distance (WD), distance between the lens and the tissue
under study, according to the size of the tissue.
Spread the black sponge sheet under the animal to obtain clear blood flow
images when the tissue does not occupy the display. The black sheet does not
reflect the laser light, and the effect makes the blood flow images clear.
6) USB key
This key allows the blood flow imager software, LSI and LIA, to work.
Insert and pull out the USB key when the power of the computer-based image
processor is off.
White dot
Shutter
knob

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2. Set up of the arm stand
The arm stand is composed of the base, the cramp, the pole and the arm.
(1) Install the cramp on the table
used for measurement.
(2) Stand the pole on the cramp
by the screws.
(3) Install the arm on the pole.
Screws
Arm

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The hold plate with the CMOS
camera.
(4) Install the hold plate with the
CMOS camera on the arm stand by
the four screws.
(5) Attach the lens on the CMOS
camera.
Lens