Pall iCELLis 500+ User manual
Filtration. Separation. Solution.SM
1.1.1.1
Instructions for Use Part A: Hardware USD3310
iCELLis®500+ Bioreactor Control System
Part Number: ICL500CSSSIP
Instructions For Use Part A: Hardware USD 3310
iCELLis®500+ control system
Part Number: ICL500CSSSIP
Page 2of 48
Table of Contents
1. Introduction ________________________________________________________ 4
2. Warnings __________________________________________________________ 5
2.1 Hazard Icons ______________________________________________________________5
2.2 Hazard Level ______________________________________________________________6
2.3 Safety Messages ___________________________________________________________7
3. Overview _________________________________________________________ 10
3.1 iCELLis 500+ Single-Use Fixed-Bed Bioreactor___________________________________10
3.2 iCELLis 500+ Control System_________________________________________________11
4. iCELLis 500+ Control System Environment Requirements ________________ 15
5. Unpacking and Installing the iCELLis 500+ Control System _______________ 16
5.1 Unpacking________________________________________________________________16
5.2 Moving and Positioning the iCELLis 500+ Control System __________________________18
5.2.1 Unlocking Load Cells______________________________________________________________ 18
5.2.2 Locking in Position and Levelling the System ___________________________________________ 19
5.3 Fitting High Speed Pumps on the iCELLis 500+ Control System______________________20
5.4 Fitting the Base Scale on the iCELLis 500+ Control Aystem _________________________21
5.5 Fitting the Tubing Holders on the iCELLis 500+ Control System ______________________22
5.6 Fitting Communication and Sensor Cables to the iCELLis 500+ Control System _________23
5.6.1 Connecting the Double Jacket Pressure Sensor Cable: ___________________________________ 23
5.6.2 Connecting the Bioreactor Pressure Sensor Cable: ______________________________________ 23
5.6.3 Connecting the T1 and T2 Temperature Probes: ________________________________________ 25
5.6.4 Connecting Optical Fibers for pH and DO: _____________________________________________ 26
5.6.6 Connecting the Filter Heater:________________________________________________________ 28
5.6.7 Connecting the Bag Pre-Heater _____________________________________________________ 30
5.7 Installation of the Temperature Control Unit (TCU) ________________________________31
5.8 iCELLis 500+ Control System Electrical Installation________________________________35
5.8.1 Electrical Power Requirements ______________________________________________________ 35
5.8.2 Power Connection and Powering ON _________________________________________________ 35
5.9 Fitting the Gas Supplies to the iCELLis 500+ Control System ________________________36
6. iCELLis 500+ Control System Specifications____________________________ 38
6.1 General Specifications ______________________________________________________38
6.2Sensors and Actuators Specifications __________________________________________41
6.3 Temperature Control Unit Specifications ________________________________________42
7. Cleaning__________________________________________________________ 43
8. Maintenance ______________________________________________________ 44
Page 4of 48
1. Introduction
All information presented in this document has been verified according to applicable standards and
guidelines at the time of writing. All legal and other binding regulations for personnel protection,
accident prevention and protection of the environment applicable in the country of use of the system
must be observed.
This instructions manual is intended to describe and explain the iCELLis 500+ bioreactor control
system functions in standard configuration and general operating conditions. It provides information
required for installation and operation of the system in these conditions. Read the manual carefully
before operating the iCELLis 500+ bioreactor control system.
The Instructions for Use (IFU) must be read, understood and applied by all personnel authorized to
operate, clean, service and maintain the system.
CAUTION!
The iCELLis 500+ control system must be used in accordance with instructions detailed in this manual.
Utilization must be carried out only by authorized persons trained to the iCELLis 500+ control system
operation.
Operation of the equipment may require other devices, instruments or safety equipment than the one
described in this Instruction for Use. This document does not include detail about such conditions and
related obligatory guidelines.
Warnings and safety messages are applicable only to iCELLis 500+ control system.
The figures, illustrations, parameters values and settings shown in this document are only examples
and should not be used for a given application. Exact settings are provided through configuration
documents or should be obtained empirically for a specific application.
This Instruction for Use has been released for the iCELLis 500+ control system hardware with the
iCELLis 500+ software version 1.03.
The iCELLis 500+ control system serial number can be found on the model plate located at the back
of the equipment. The iCELLis 500+ software version can be found in Software HEADER after
start-up of the controller.
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2. Warnings
2.1 Hazard Icons
The following icons are used in this Instruction for Use to point out hazards resulting from the utilization
of the system described.
Documentation must be carefully assessed where these icons are present.
General icon for DANGER, WARNING, CAUTION or ALERT
This symbol notifies general risk or specific procedures, which if
not followed correctly, can result in injury of personnel or damage
to equipment.
Biological hazard DANGER icon
This symbol notifies a biological hazard risk.
Electrical Shock WARNING icon
This symbol notifies a risk of electric shock.
Crush WARNING icon
This symbol notifies a risk of a crushing event.
Hot Surface WARNING and CAUTION icon
This symbol notifies a risk of burn.
Magnetic field DANGER icon
This symbol notifies a risk linked to magnetic fields.
WARNING Risk of hand entanglement and crush
These symbols notify a risk of hand or clothing entanglement.
Red icon is displayed on peristaltic pumps.
Information icon
This symbol is used to highlight useful information and indicate that
caution should be exercised while working on the system.
CAUTION Do not cover during operations
Safety pressure relief valve output. Do not cover during operations.
WARNING Hazardous voltage inside
Risk of electrical shock. Disconnect power before opening the doors.
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CAUTION Tip over hazard
Risk of tipping of the accessories pump. Do not lean on the pump
head of the accessories pumps.
CAUTION Surface may be hot inside.
Risk of burn. Allow to cool before opening the bag pre-heater.
CAUTION Strong magnetic field.
Interaction with metallic objects may produce Pinch Hazards.
Persons with medical implants KEEP BACK 12 in. (30 cm) from the
agitator shaft.
CAUTION wiring color differs from the NFPA 79 Standards.
The electric wire colour used in the iCELLis 500+ control system is
listed on the label and conformed to the IEC 60445.
2.2 Hazard Level
Hazard icons are used in combination with the following danger level indications:
DANGER!
Will lead to severe injury or death
WARNING!
May lead to severe injury or death
CAUTION!
May lead to light or moderate injury
ALERT!
May lead to material or data damage
IMPORTANT!
May lead to inconsistency in system utilization
Hazard or information icons may be supplemented with specific description or instructions.
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2.3 Safety Messages
DANGER! Risk of injury to personnel and/or damage to equipment!
Before using the iCELLis 500+ control system, ensure you read and understand the safety instructions
to prevent any damage to the equipment or any physical injury. Pall shall not be held responsible for
any damage that may result from misuse. Misuse includes any use other than that designed for the
iCELLis 500+ bioreactor and control system. Keep this guide handy for later reference. In the event
you have additional questions, please contact Pall.
DANGER! Risk of injury to personnel and/or damage to equipment!
Use appropriate personal protective equipment (PPE) when moving and using the iCELLis 500+
control system.
DANGER! Biological hazard!
The biological hazard is a function of the process performed and is under the responsibility of the user.
Appropriate safety risk analysis, depending on the biological material used, should be performed by
the user. Appropriate personal protective equipment must be used.
WARNING! Risk of injury to personnel and/or damage to equipment!
The iCELLis 500+ control system must be operated by qualified people within a safe environment only.
This equipment is expected to be installed in a controlled environment. Do not use the equipment in
hazardous locations, such as Atmosphere Explosive (ATEX) Environmental operating conditions:
18 - 25 °C, 10 - 70 % relative humidity non-condensing. When not in use, the iCELLis 500+ control
system must be stored in temperatures ranging from 4 ºC - 40 ºC, 80% maximum relative humidity
non-condensing. Keep the iCELLis 500+ control system in a dry place.
ALERT! Risk of damage to equipment!
This equipment has been designed for indoor use only.
DANGER! Risk of injury to personnel and/or damage to equipment!
Before installing the iCELLis 500+ control system, ensure that the floor can support the weight of the
system, the ancillary equipment and their content (such as cell culture media).
ALERT! Risk of damage to equipment!
Never use the equipment in a manner not specified by or clearly prohibited by the manufacturer.
Such inappropriate use may impair the equipment’s built-in protections and would void the warranty.
ALERT! Risk of damage to equipment!
Do not use the iCELLis 500+ control system in an incubator.
ALERT! Risk of damage to equipment!
Never position the equipment so that it is difficult to access the machine main power switches, gas inlet
ports and emergency stop buttons. A minimum clearance of 650 mm around the system (front, rear,
left and ride sides) is needed.
WARNING! Risk of injury to personnel and/or damage to equipment!
Refer to the operating manual of the Temperature Control Unit (TCU) provided within the
documentation of the iCELLis 500+ control system.
Make sure you read and understand all instructions and safety precautions listed in the operating
manual of the TCU before installing or operating your unit.
WARNING! Risk of injury to personnel and/or damage to equipment!
Place the TCU on an even surface with a base made of nonflammable material.
WARNING! HOT/COLD surfaces! Risk of injury to personnel and/or damage to equipment!
Always empty the bath of the TCU before moving the unit. Do not drain the bath fluid while it is hot!
Check temperature of bath fluid prior to draining (e.g. by switching the unit on for a short moment).
WARNING! Bended tubing! Risk of injury to personnel and/or damage to equipment!
Avoid sharp bends in the fluid tubing of the TCU and maintain a sufficient distance from surrounding
walls. Make sure that the tubing is securely attached.
CAUTION! Risk of injury to personnel and/or damage to equipment!
When the TCU, media pre-heater or the filter heater are active the surfaces and tubes can be hot.
Therefore, exercise particular caution when handling.
ALERT! Risk of damage to equipment!
Do not disconnect the communication cable between the iCELLis 500+ control system and the TCU
Page 8of 48
during operation.
DANGER! Oxygen causes a fire/explosion risk!
Users are responsible for performing their own safety assessment and obtaining expert advice on the
design of any system used to supply gases to the iCELLis 500+ control system.
RELEVANT ONLY WHEN USING OXYGEN: In the unlikely event of a tubing or connection failure,
oxygen may build up in and around the iCELLis 500+ control system. A means of independently
monitoring oxygen close to the equipment is highly recommended.
The monitoring system should be set up to prevent oxygen levels from reaching 24% or limits
recommended by local regulations. In the event of a rise in oxygen above this level, the supply should
be immediately shut down. The iCELLis 500+ control system can deliver oxygen up to 7 L/min. Oxygen
supply to the iCELLis 500+ control system is the user’s responsibility.
DANGER! Risk of asphyxiation!
Carbon dioxide can cause asphyxiation. Ensure that the work space has adequate ventilation. Do not
place the system in an enclosed area. The iCELLis 500+ control system can deliver carbon dioxide up
to 1.5 L/min. Carbon dioxide supply to the iCELLis 500+ control system is the user’s responsibility.
DANGER! Risk of asphyxiation!
Nitrogen can cause asphyxiation. Ensure that the work space has adequate ventilation. Do not place
the system in an enclosed area. Note that the iCELLis 500+ control system can supply nitrogen up to
1.5 L/min. Nitrogen supply to the iCELLis 500+ control system is the user’s responsibility.
WARNING! Risk of injury to personnel and/or damage to equipment!
Gases supplied to the iCELLis 500+ control system must be regulated to specified pressure (maximum
2.5 barg or 36.3 PSIG) and must be oil-free, dry and clean (medical grade). To prevent possible
system component damage, do not exceed these recommendations. Each gas line should be supplied
with an ON/OFF valve and an independent regulator.
WARNING! Risk of injury to personnel and/or damage to equipment!
User is responsible for performing assessment on the risks linked to liquids used in the iCELLis 500+
control system and bioreactor. Appropriate personal protective equipment must be used.
WARNING! High voltage!
Always make sure this equipment is properly earthed/grounded.
WARNING! Risk of injury to personnel and/or damage to equipment!
Only use the mains supply cord that is supplied with the iCELLis 500+ control system.
WARNING! Risk of injury to personnel and/or damage to equipment!
When the iCELLis 500+ control system is set to manual mode, it will not be controlled by the
automation logic. Product damages or personal injuries may result!
WARNING! Risk of injury to personnel and/or damage to equipment!
Never spray liquid or allow ingress of fluid into the fans of the iCELLis 500+ control system.
WARNING! Risk of injury to personnel and/or damage to equipment!
Before each maintenance, cleaning or moving operation, turn the iCELLis 500+ control system OFF,
unplug the system from the AC power, shut down the gas supply, disconnect gas lines, lock the
loadcells, remove the bioreactor, remove the manifolds and all liquids.
WARNING! Risk of injury to personnel and/or damage to equipment!
In case of leak of liquid appropriate actions must be taken for user safety according to applicable
procedures and national rules.
WARNING! Risk of injury to personnel and/or damage to equipment!
The crated iCELLis 500+ control system should always be lifted in the upright position. The hardware
should only be lifted from a side. Before lifting, ensure that the lifting device forks pass all the way
under the box section of the hardware.
WARNING! Risk of injury to personnel and/or damage to equipment!
The repositioning of the iCELLis 500+ control system must be done by two or more people due to the
weight of the system.
WARNING! Risk of injury to personnel and/or damage to equipment!
Manipulation and repositioning of the bioreactor on and off the iCELLis 500+ control system docking
station must be completed when the iCELLis 500+ bioreactor is completely empty and must be done
by two people due to the weight of the bioreactor.
Page 9of 48
WARNING! Risk of injury to personnel and/or damage to equipment!
The positioning of the accessories pumps (optional FILL and DRAIN pumps) must be done by two
people due to the weight of the pump.
WARNING! Risk of injury to personnel and/or damage to equipment!
Accessories pumps (optional FILL and DRAIN pumps) must be properly placed and stability of the
pump must be checked before operation.
CAUTION! Risk of entanglement and crush injury when using peristaltic pumps!
Do not allow fingers or clothing to contact moving parts of the peristaltic pumps.
Keep peristaltic pumps cover closed when pumps are operating.
User must stop the peristaltic pumps before installing and removing tube from peristaltic pump heads.
ALERT! Risk of damage to equipment!
Only the iCELLis 500+ bioreactor provided by Pall should be used with the iCELLis 500+
control system.
ALERT! Risk of damage to equipment!
Never block the cooling fan outlets and cooling air inlet on the iCELLis 500+ control system when the
iCELLis 500+ control system is powered.
ALERT! Risk of damage to equipment!
Only exhaust filter heater provided by Pall should be used with the iCELLis 500+ control system.
ALERT! Risk of damage to equipment!
The iCELLis 500+ control system should never be moved when there is liquid in the bioreactor.
ALERT! Risk of damage to equipment!
NEVER restrict flow of gas through the exhaust lines of the iCELLis 500+ bioreactor (gas out line).
ALERT! Risk of damage to equipment!
The iCELLis 500+ control system is equipped with load cells under the docking station for bioreactor
weight measurement. Load cells must be secured using supplied locking plates during control system
transport and handling in order to avoid damage.
Locking plates must be removed prior to use of the system.
WARNING! Magnetic field! Risk of injury to personnel!
The iCELLis 500+ control system and iCELLis 500+ bioreactor contain strong magnetic elements.
Persons with certain metallic, electronic, magnetic, mechanical implants, devices, or objects may not
enter this area.
ALERT! Risk of damage to equipment!
Do not tilt the iCELLis 500+ bioreactor during the draining of the double jacket while it is on the control
system. The load cells can be damaged.
Page 10 of 48
3. Overview
The iCELLis 500 bioreactor platform is a high-cell density culture system designed for animal cell
culture under large scale Good Manufacturing Practices (GMP) production. It consists of a single-use
fixed-bed bioreactor and a docking station including controlling system with integrated measuring,
agitation, temperature control and pumping elements to operate the bioreactor.
3.1 iCELLis 500+ Single-Use Fixed-Bed Bioreactor
The iCELLis 500+ bioreactor is fully sinle-use including its sensors. The bioreactor vessel contains a
pre-packed fixed-bed made of Pall’s proprietary iPack carriers designed for both suspension and
adherent cells which grow entrapped in the fixed-bed. This matrix is made of medical grade
polyethylene teraphalate (PET). microfibers.
The volume of the fixed-bed (number of carriers) varies depending on the bioreactor model but the
outer shell (body) of the bioreactor does not change with the fixed-bed volume and offers a maximum
working volume of ~74 L (Further media volume can be added through perfusion/ recirculation). Hence,
the same docking and controlling station is used with all iCELLis 500+ bioreactors.
The volume in the bioreactor which the fixed-bed occupies varies from 5 to 25 L offering a cell
adherence equivalent surface area between 66 to 500 m² respectively. A surface area of 500 m² is
equivalent to ~3,000 roller bottles (1700 cm² each).
The main elements of the iCELLis 500+ bioreactor are:
•A body made of Polyethylene Terephthalate Glycol (PETG) parts, including a double-jacket for
temperature control;
•A fixed-bed made of PET carriers;
•Different tubing and accessories such as integrated single use pH, DO, pressure and optional
Biomass sensors (Figure 1).
The bioreactor is manufactured and assembled in accordance with ISO 14644-1 Class 8 cleanroom
guidelines. Unless specifically marked otherwise, the bioreactor is delivered gamma-irradiated at a
minimum of 25 kGy (range of 25 to 50 kGy). The product label includes a gamma exposure indicator
(color change gamma-exposure indicator).
Page 11 of 48
Figure 1: iCELLis 500+ bioreactor
3.2 iCELLis 500+ Control System
The iCELLis 500+control system was specifically designed to operate, monitor and control the
iCELLis 500+ bioreactors. The system body is composed of grade 304/316 L stainless steel and
houses the following elements (Figure 2):
•A bottom assembly with controller PLC, agitation motor, electrical connection, media preheater,
and the bioreactor docking station with load-cells; optionally two accessory peristaltic pumps
can be added in dedicated compartments;
•A middle assembly with gas management block;
•A top assembly with pumps, probe connections, probe transmitters, electrical connections,
emergency stop buttons and the touch screen panel;
•An external TCU for bioreactor temperature control.
The bioreactor is placed on the docking station, which contains the load-cells and a magnetic
coupling for the agitation. When installed on the docking station, the bioreactor is directly under the
sensor connections.
Process control is performed through a dedicated interface (iCELLis 500+ software) and can be
accessed locally through the touch-screen panel or remotely. The iCELLis 500+ software has been
developed specifically for iCELLis 500+ platform in order to make the equipment compatible with
authorities’ standard guidelines for electronic records and signatures in biopharmaceutical processes
(21 CFR Part 11, Eudralex Annex 11).
Page 12 of 48
Figure 2: iCELLis 500+ control system
An overview of the iCELLis 500+ control system components in front, rear, right and left views are
presented in Figure 3, Figure 4 and Figure 5.
Optional High Flow
peristaltic pumps
Touch
screen
panel
Middle assembly
Top assembly
Bottom assembly
Docking station
Page 13 of 48
Figure 3: iCELLis 500+ control system front view
Figure 4: iCELLis 500+ control system rear view
Page 14 of 48
Figure 5: iCELLis 500+ control system right and left views
Page 15 of 48
4. iCELLis 500+ Control System Environment Requirements
WARNING! Risk of injury to personnel and/or damage to equipment!
The iCELLis 500+ control system is not ATEX certified. The system may not be operated in
potentially explosive atmosphere.
DANGER! Risk of injury to personnel and/or damage to equipment!
Before installing the iCELLis 500+ control system, verify that the floor can support the weight of the
system, the ancillary equipment and their content (such as cell culture media).
ALERT! Risk of damage to equipment!
This equipment has been designed for indoor use only and a maximum ambient environmental
temperature of 25 °C.
The dimensions and weight of the iCELLis 500+ control system can be found in Table 1 below.
Table 1: iCELLis 500+ control system dimensions and weight
Description
Dimensions and Weight
Height
1850 mm (excluding visual alarm and tubing holders)
2122 mm (with visual alarm and tubing holders)
Width
955 mm (excluding optional high flow pump)
1038 mm (with optional high flow pump)
Length
1609 mm
Dry Weight
(without bioreactor)
650 kg
Maximum weight when loaded
(with bioreactor full of media and
double jacket full of water)
< 850 kg
Environmental working conditions of the iCELLis 500+ control system are detailed in Table 2 below.
Table 2: iCELLis 500+ control system environmental working conditions
Description
Environmental Working Conditions
Environment condition
Indoor use only
Maximum Altitude
2000 m
Ambient operating temperature
18 °C to 25 °C
Relative operating humidity
10 - 70%, non-condensing
Pollution degree
2
IP rating
55
The work area where the iCELLis 500+ control system is installed and operated must be leveled and be
able to accommodate the footprint and weight of the iCELLis 500+ control system with a 650 mm
minimum clearance on each side of the system to enable access to the controller buttons (such as
emergency stop buttons) and other inlets/sockets.
Page 16 of 48
5. Unpacking and Installing the iCELLis 500+ Control System
The required materials for unpacking and installing the iCELLis 500+ control system are detailed below:
•Forklift with minimum fork length of 1.4 m capable of lifting 1.320 kg at the full length of
the forks.
•Stepladder, scissors, spirit level, spanner (wrench):13 and 24 mm, adjustable spanner: jaw
capacity 25 - 26 mm, Metric hex keys: 4 mm, 5 mm and 6 mm, small flat screw driver
5.1 Unpacking
WARNING! Risk of injury to personnel and/or damage to equipment!
The crated iCELLis 500+ control system should always be lifted in the upright position. Once outside
the crate, the hardware should only be lifted from the side. Before lifting, ensure that the lifting device
forks pass all the way under the box section of the hardware.
ALERT! Risk of damage to equipment!
Before placing the iCELLis 500+ control system on the floor, be sure the floor can support the weight
of the system.
ALERT! Risk of injury to personnel and/or damage to equipment!
Never lift the iCELLis 500+ control system by the top.
The iCELLis 500+ control system is shipped within 2 wooden crates with the following dimensions
and weight:
Table 3: Weight and dimensions of iCELLis 500+ control system crates
iCELLis 500+ Control System
Accessories and Documentation
Crate weight
~850 kg
~300 kg
Crate dimensions
(H x W x L)
2135 x 1120 x 1760 mm
1800 x 800 x 1200 mm
Inspect the crate to ensure it has not been damaged, also check the ‘TIP N TELL’ and Shock indicator
(if applicable) located on the side of the crate for signs that the crate has been tipped or mishandled.
It is recommended that two persons uncrate the iCELLis 500+ control system together. The four sides,
top and bottom sections of the crates are held together using the fixings shown in Figure 6b.
To uncrate: Remove one of the larger side panel then remove the top panel of the crate and finish with
the remaining vertical panels. Remove the first layer of plastic wrap, then the wooden support for the
top assembly and the second wrap (Figure 6 f-h). Proceed similarly with accessories and
documentations crate (Figure 6i).
Lift the iCELLis 500+ control system from the base of the box using a forklift at the lifting points on the
side of the unit (Figure 6h). Ensure the forks are positioned under the controller framework prior to
lifting. Lifting in any other way may cause damage to the hardware. Once the iCELLis 500+ control
system has been lifted move the crate base and place the iCELLis 500+ control system on the floor.
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Figure 6: Uncrating the iCELLis 500+ control system – a) Crated unit, b) Removing crate fixing clips, c) Lifting
side panel, d) Removing side panel, e) Side panel removed, f) Unit with double plastic wrap, g) Unit with external
wrap removed, h) Unit unwrapped and lifting points (blue), i) Crate for accessories and documentation.
a)
b)
c)
d)
e)
f)
g)
h)
i)
Page 18 of 48
5.2 Moving and Positioning the iCELLis 500+ Control System
WARNING! Risk of injury to personnel and/or damage to equipment!
Manipulation and repositioning of iCELLis 500+ control system must be completed when the
iCELLis 500+ bioreactor is completely empty and must be done by two or more persons due to the
weight of the bioreactor.
DANGER! Risk of injury to personnel and/or damage to equipment!
Before installing the iCELLis 500+ control system, verify that the floor can support the weight of the
system, the ancillary equipment and their content (such as cell culture media).
WARNING! Risk of injury to personnel and/or damage to equipment!
Before each moving operation, turn the iCELLis 500+ control system OFF, unplug the system from
the AC power, shut down gas supply, disconnect gas lines, remove the bioreactor, remove the
manifolds and all liquids.
WARNING! Risk of injury to personnel and/or damage to equipment!
The iCELLis 500+ control system must stand on its feet. Feet must be locked and leveled prior to
powering the unit.
The iCELLis 500+ control system can be moved on its castors. Only the bottom assembly must be
used to push or pull the unit. Do not push or pull from the docking station. Please do not move the
equipment in any other way than instructed.
Unlocking Load Cells
ALERT! Risk of damage to equipment!
The iCELLis 500+ control system is equipped with load cells under the docking station for bioreactor
weight measurement. Load cells must be secured using the supplied locking plates during control
system transport and handling to avoid damage.
Locking plates must be removed prior to use of the system.
ALERT! Risk of damage to equipment!
Do not tilt the iCELLis 500+ bioreactor on the docking station during draining of the double jacket. Load
cells can be damaged.
Please do not discard locking plates and store them in a safe place to be able to lock the load cells
during future transport and handling of the iCELLis 500+ control system.
The iCELLis 500+ control system is equipped with 3 load cells which are locked during transport
(Figure 7a).
Remove the locking plate for each load cell after the control system has been installed at its final
location. Unscrew the Anti-Lift bolt with a 13 mm spanner and remove the Checking Sleeve (Figure 7b).
Remove the Centering Spacer and the Locking Plate (Figure 7c). Place back the Checking Sleeve and
the Anti-Lift Bolt (Figure 7d).
Page 19 of 48
Figure 7: Load cell locking plate removal at system reception: a) Load cell with
locking plate in place, b) Locking plate and fixation elements, c) Locking plate and bolts removed, d) Checking
sleeve and anti-lift bolt in place.
Locking in Position and Levelling the System
Once the iCELLis 500+ control system is in its required position the wheels should be turned and feet
lowered to lock the casters (Figure 8). When performing this step, a spirit level should be used and
placed on the bioreactor docking station to ensure the hardware is leveled before use. When the
iCELLis 500+ control system is leveled, lock the feet by turning the locking bolt on the caster. The feet
should always be raised prior to moving the iCELLis 500+ control system.
Figure 8: Positioning the iCELLis 500+ control system: a) level placed on docking station,
b) and c) Wheel turned and feet lowered to lock casters.
Page 20 of 48
5.3 Fitting High Speed Pumps on the iCELLis 500+ Control System
WARNING! Risk of injury to personnel and/or damage to equipment!
The positioning of the high-speed pumps must be done by two people due to the weight of the pumps.
ALERT! Risk of damage to equipment!
Always check the position and stability of the accessories pump before operation.
WARNING! Risk of injury to personnel and/or damage to equipment!
Before installing the accessories pump, turn the iCELLis 500+ control system OFF, unplug the system
from the AC power, shut down gas supply, disconnect gas lines, remove the bioreactor, remove the
manifolds and all liquids.
The high-speed pumps are packed in the crate for accessories and documentation. Unpack each high
speed pump, and install the pump in its compartment (Figure 9a) prior to connecting to the iCELLis
500+ control system as per the Figure 7b. Connect the power cable (Figure 9c) and the communication
cable (Figure 9d) to the iCELLis 500+ control system. Power to the pumps is supplied by the Control
system.
Figure 9: Placing and connecting High Speed pump: a) High speed pump compartment,
b) High speed pump in compartment, c) Connection of power plug, d) Connection of communication port.
This manual suits for next models
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Table of contents
