PendoTECH UV-VIS-NIR User manual

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 1of 24
Document Title: UV-VIS-NIR Photometer P-Version User’s Manual
PENDOTECH® UV-VIS-NIR
PHOTOMETER FOR UV
ABSORBANCE AND TURBIDITY
MEASUREMENTS
USER’S MANUAL
PendoTECH
www.pendotech.com

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 2of 24
Disclaimer
All rights reserved. No part of this publication may be reproduced, stored in an electronic retrieval
system, or transmitted, in any form or by any means, whether electronic, mechanical, by photocopying,
or otherwise, without the written consent of PendoTECH.
The information in this User Guide is believed to be accurate and reliable for use and operation of the
monitor, however, PendoTECH assumes no responsibility for the use of this product except for what is
covered in the Limited Warranty and Terms and Condition of Sale.
NOTE: “NOTE” is used to notify the user of installation or operation information which is important but
not hazard related.
Safety Guidelines
Warning potential shock hazard. Do not submerge this product. Protect the product
before cleaning with any liquids by covering openings that expose the internal
components.
Each prospective user must test the measurement unit for its proposed application to determine its
suitability for the purpose intended prior to incorporating the sensor to any process or application. The
measurement unit is not intended for use as a component in life support. The sensor is not designed for
any application in which the failure of the product could result in property damage, personal injury, or
death. Proper safeguards must be put into place for the process in which the unit is used.
This device has left our facility after careful testing of all the photometer’s functions and safety features.
The functioning and operational safety of the product can only be ensured if the user observes the usual
safety precautions as well as the specific safety guidelines stated in these operating guidelines:
Before connecting the device to the electrical
supply, ensure that the operating voltage
stated on the power supply corresponds to
the voltage supplied to the unit.
The functioning and operational safety of the
instrument can only be maintained under the
conditions specified in the specifications
section of this manual.
If the instrument is moved from warm
surroundings, condensate may form and
interfere with the functioning of this
instrument. In this event, wait until the
temperature of the photometer equilibrates
to the new temperature before putting it back
into operation.
If there is any reason to assume that the
product can no longer be employed without
risk, it must be set aside and appropriately
marked to prevent further use.
The safety of the user may be endangered if
the instrument:
•is visibly damaged
•no longer operates as specified
•has been damaged in transport
If you are in doubt, the product should be
sent back to the factory.
The operator of this product must ensure that
the following laws and guidelines are
observed when using this product around
dangerous substances:
•EEC Directives
•National Fire Protection Association
•Safety data sheets of the chemical
manufacturer
Maintenance, and repair work must only be
carried out by PendoTECH

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 3of 24
Optical Safety
WARNING: The photometer can emit light from ≈200 – 1100nm . Hazardous UV and IR radiation
are emitted from the unit. Never directly stare into the source/return ports on the photometer or
the fiber optic cables, as this can cause eye damage. The viewer-related risk is dependent on how
the users install and use the product.
Ensure that both ends of the fiber optic cables (photometer and flow cell connections) are
properly secured (firmly hand tight) before powering on the photometer.
The photometer should always be powered off when handling the fiber optic cables. If you must
handle the fibers when the unit is powered on, protective eyewear must be worn.
In the event of product failure, do not attempt to open the unit or replace the LED. There are no
user-serviceable parts.
This warning label shown below can be found on both the photometer and the fiber optic cables
ends. Users should take precautions and safety measures for both UV and IR radiation:

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 4of 24
Notice of Confidentiality
The information contained within this document is confidential and proprietary to PendoTECH and may
be covered under existing US patents or patents pending. This information shall not be reproduced or
further disclosed, in whole or in part, to anyone other than employees of the company purchasing the
product without prior written consent from PendoTECH.

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 5of 24
Contents
Section 1: Introduction and Unpacking ......................................................................................................... 6
Section 2: Hardware Installation ................................................................................................................... 8
Section 4: Glossary and Definitions ............................................................................................................. 16
Section 5: Instrument Controls ................................................................................................................... 17
Section 6: Absorbance Operations ..............................................................................................................19
Section 7: System Specifications ................................................................................................................. 20
Section 8: Appendix..................................................................................................................................... 21

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 6of 24
Section 1: Introduction and Unpacking
PendoTECH’s UV-VIS-NIR Photometer is a measuring and monitoring device that allows users to
make in-line absorbance and turbidity measurements from bioprocess fluid streams. The photometer also
acts as a transmitter with a 4-20mA output scaled 0-3 AU. The absorbance measurements are made by a
collimated beam of light which passes through a sample with a defined path length. The absorbance is
determined as the ratio of the light applied from the source to what passed through the sample. At
wavelengths below 400nm, the photometer can be used to measure the UV absorbance of a liquid
solution to identify the absence or presence of a molecule of interest. At wavelengths greater than
400nm, the photometer can be used to measure turbidity as an indication of filter performance or to
measure unclarified material. The photometer also comes in a dual wavelength version, which allows
users to make two measurements of different wavelengths (from 255 to 1000nm) simultaneously in the
same sample.
The combination of the UV-VIS-NIR photometer and the PendoTECH Single Use Flow Cells allow
the measurements to be made in-line. The flow cells contain a special silica glass lens and compartments
to insert optical couplers which attach to the fiber optic cables from the photometer. The flow cells can
also be used with PendoTECH’s Flow Cell Stand with integral couplers in place of the optical couplers to
connect the fiber optic cables. The flow cell stand is optional for UV absorbance applications, but it is
highly recommended for turbidity applications because it blocks out ambient light that would otherwise
affect the readings. Although they are designed for single use, the flow cells may be repeatedly cleaned
and re-used.
Identify the following components:

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 7of 24
3. Optical Couplers/Flow Cell Holders (Varies per flow cell used and configuration type)
Optional Flow Cell Stand
4. Example Flow Cells:
Single Use Flow Cell, 1cm path length
Adjustable Path Length Flow Cell,
coupler removed
Adjustable Path Length Flow Cell,
coupler inserted

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 8of 24
Section 2: Hardware Installation
Please follow the outlined sections below as a guideline for installing the photometer and associated
accessories. These guidelines apply for specific application installations or in the case of OEM component
installation onto a suitable panel.
2.1 Environmental
It is strongly recommended that the photometer be installed in a clean, dry area where ambient
temperature does not exceed 115F/46C. Systems mounted in enclosures can be purged with clean, dry,
oil-free air (or nitrogen) to dissipate heat within the enclosure.
2.2 Physical
For permanent installations, bolt the photometer, back panel, or enclosure (as appropriate) in place using
mounting screws. The location should be secure, rigid, and strong enough to support the weight of the
installed system.
For laboratory use, ensure that there is sufficient space on the bench or tabletop to accommodate the
hardware supplied.
For all systems, run the fiber optic cable(s) through conduit or other appropriate protective measures as
desired. Refer to Section 2.3 below for optical component installation instructions.
Run required electrical wiring for instrument power and output signals (4-20mA). Review Section 2.4
below prior to making electrical connections.
2.3 Optical
DO NOT SUPPLY POWER TO THE UNIT UNTIL TOLD TO DO SO IN THE
INSTRUMENT SETUP AND INITIALIZATION PROCEDURE IN SECTION 6.

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 9of 24
Photometer, front view
Complete system setup showing measure unit/photometer, flow cell stand/holder, and fiber optic cables
1. Remove the protective caps from the SMA-905 connectors on the fiber optic cables and the
photometer.
2. Clean the fiber ends with spectroscopic-grade isopropyl alcohol (IPA, also known as 2-propanol)
or methanol using a lint-free cotton swab or non-abrasive task wipe. Spectroscopic grade acetone
can also be used, but separation of cotton from the swab might occur as the binding agent
dissolves.
3. Properly mount the flow cell stand/holder (if applicable to system). The flow cell stand can be set
directly flat on a lab bench, or should be properly wall/panel-mounted. Proper mounting of the
flow cell stand facilitates the pass through of bubbles/air pockets in the fluid stream, which if
trapped in the flow cell, can degrade system performance.
SPEC-FCH-L Panel Mounting
SPEC-FCH-S Panel Mounting

PendoTECH UV-VIS-NIR Photometer for UV
Absorbance and Turbidity Measurements User’s Manual
Revision 3
Page 10 of 24
4. Connect the flow cell with optical couplers or flow cell stand to the photometer with the fiber
optic cables. It does not matter which fiber connects to the source and return.
NOTE: Ensure that both ends of the fiber optic cables (photometer and flow cell connections) are
properly secured (firmly hand tight) before powering on the photometer.
5. Power on the unit. Refer to section 2.4 below.
6. Press the tare button (or perform dry contact closure over Tare/Zero +/- pins). This initiates the
calibration and taring routine. The status light will turn green.
a. Note: For best results, the unit should be tared/calibrated with the process background
fluid in the flow cell if possible. If not possible, then a tare/calibration with air in the flow
cell may be used instead.
7. Wait at least 10 seconds for the taring and calibration routine to complete. The status light will
turn back blue.
a. Note: In a panel mount installation, the status LED is not visible. It is recommended to
integrate a 10s indication display on the user interface that the unit is not ready for use
(do not change fluid sate in the flow cells light path).
8. The unit is now ready for operation.
NOTE:The fiber optic cables must be secured so they are not free to
move around, be stepped on, leaned against, or otherwise damaged
during normal operations. The minimum bend radius for fibers
supplied with the UV-VIS-NIR Photometer is 8” (20.3cm).
NOTE: Only hand tighten SMA-905 connections. Never use a wrench,
pliers, or other tool. Over-tightening the SMA-905 connections may
result in damage to the connector and the fiber optic cable,
drastically reducing or prohibiting light transmission and requiring the
replacement of the fiber optic cables.