Hanna Instruments HI 99181 User manual

HI99181
Portable Waterproof
Skin pH Meter
INSTRUCTION MANUAL

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before
using this meter.
This manual will provide you with the necessary
information for correct use of this meter, as well as a
precise idea of its versatility.
If you need additional technical information, do not
hesitate to e‑mail us at [email protected] or view
our worldwide contact list at www.hannainst.com.
Dear
Customer,
All rights are reserved. Reproduction in whole or in part is prohibited
without the written consent of the copyright owner, Hanna Instru-
ments Inc., Woonsocket, Rhode Island, 02895, USA.

3
TABLE OF CONTENTS
Table of Contents......................................................
Preliminary Examination ...........................................
General Description and Intended Use........................
Main Features..........................................................
Specifications...........................................................
Display Description ...................................................
Operational Guide ....................................................
Meter Setup.............................................................
Battery Replacement.................................................
Accessories...............................................................
Electrode Maintenance..............................................
Certification..............................................................
Recommendations for Users ......................................
Warranty .................................................................
4
5
7
8
15
6
8
16
19
14
17
3
18
19

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PRELIMINARY EXAMINATION
Remove the meter from the packing material and examine
it carefully to make sure that no damage has occurred
during shipment. If noticeable damage is evident, contact
your local Hanna Instruments Office.
Each meter is supplied complete with:
• HI14143/50 pH/temperature probe with flat tip,
50 mm‑long body, DIN connector and 1m (3.3’) cable
• pH 4.01 & 7.01 Buffer sachets
• HI700620 Cleaning and disinfection solution for skin
residual (1 sachet)
• HI700621 Cleaning solution for skin grease and
sebum (1 sachet)
• 100 mL beaker (1 pcs.)
• Alkaline batteries: 1.5V AAA (3 pcs.)
• Rugged carrying case
• Calibration certificate of meter
• Calibration certificate of probe
• Instruction manual
Note: Save all packing material until you are sure that
the meter functions correctly. All defective items must
be returned in the original packing together with the
supplied accessories.

5
HI99181 is a light weight, portable, pH and temperature me‑
ter supplied with a specially designed pH electrode intended
for the direct determination of pH on skin and scalp.
Researchers monitoring the compatibility between skin and
cosmetics or pharmaceuticals use pH as essential marker of
compatibility. The skin mantle has an acidic pH, ranging from
pH 4 to 6. The acidic pH is a deterrent toward harmful mi‑
crobes, pollution, and toxins. Age, genetics, sweat, and mois‑
ture can alter the pH of skin. Products are constantly created
to restore the pH balance of skin and a reliable pH measure‑
ment of skin provides the scientific metrics.
The HI99181 portable pH meter together with a HI14143/50
combination pH electrode (when immersed in a drop of water
on the skin surface), can determine the pH of the skin with
high accuracy and repeatability.
The HI99181 features two button operation and is simple
to use. The HI99181 has a waterproof and compact casing,
large dual‑line display, and automatic pH calibration at one
or two points.
The HI14143/50 offers numerous features that improve pH
testing for skin measurements. The flat tip of the HI14143/50
provides optimal contact between the sample and the sen‑
sor. The HI14143/50 features low resistance pH glass that
responds quickly to the sample. It also has an open junction
design and utilizes a non‑flowing gelled reference electrolyte
so there is no variability in flow rate affecting the measure‑
ment. Both the pH sensing surface and reference junction
come into intimate contact with the skin and equilibrate
quickly and reproducibly. It also has a lower profile for easier
handling compared to laboratory sensors; the glass body is
just 50 mm in length. The HI14143/50 pH electrode has a
built‑in temperature sensor for temperature compensated pH
and temperature readings and contains an integral pH sen‑
sor preamplifier to provide stable measurements in electrically
noisy environments.
GENERAL DESCRIPTION and INTENDED USE

6
Main features:
• Simultaneous pH and temperature measurements on a
large dual‑line LCD display
• Automatic pH calibration at one or two points within
two memorized buffer sets (standard or NIST)
• Selectable temperature unit (°C or °F)
• Electrode condition indicator
• mV of pH measurement for electrode check
• HI14143/50 dedicated pH with integral temperature
sensor
• Probe quick connect system
• Battery life indication and low battery detection
• Keystroke confirmation tone
• Auto‑off function
• Waterproof casing IP67
MAIN FEATURES

7
SPECIFICATIONS
Range*
‑2.00 to 16.00 pH / ‑2.0 to 16.0 pH
±825 mV (pH‑mV)
‑5.0 to 105.0 °C/23.0 to 221.0 °F
Resolution
0.01 pH / 0.1 pH
1 mV
0.1 °C/0.1 °F
Accuracy
@ 20°C / 68°F
±0.02 pH / ±0.1 pH
±1 mV (pH‑mV)
±0.5 °C up to 60 °C; ±1.0 °C outside
±1.0 °F up to 140 °F; ±2.0 °F outside
Temperature
compensation
Automatic
‑5.0 to 105.0 °C/23.0 to 221.0 °F
pH Calibration
Automatic, 1 or 2 point selectable buffer
set Standard: 4.01;7.01;10.01 or
NIST: 4.01;6.86;9.18
Probe (included)
HI14143/50 preamplified pH and
temperature probe with flat tip 50
mm‑long body, DIN connector and 1 m
(3.3’) cable
Battery type / life 1.5V AAA (3 pcs.)
approx. 1400 hours of continuous use
Auto‑Off user selectable: after 8 min, 60 min or
disabled
Environment 0 to 50 °C (32 to 122 °F)
RH max. 100%
Meter Dimensions 154 x 63 x 30 mm (6.1 x 2.5 x 1.2”)
Meter Mass
(with batteries) 196 g (6.91 oz.)
Case Ingress
Protection Rating IP67
* the HI14143/50 is limited to be used from 0 to 12 pH and
from 0 to 50 °C temperature (32 to 122 °F).

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1 Automatic Temperature Compensation indicator
2 Stability indicator
3 Battery percentage
4 Electrode condition indicator
5 Low battery indicator
6 Measurement unit
7 Primary LCD
8 Temperature unit
9 Secondary LCD
10 Meter modes indicator
11 pH calibration buffer(s) used
DISPLAY DESCRIPTION
Each meter is supplied with batteries. Before using the
meter for the first time, open the battery compartment
and insert batteries, observing the polarity (see “Battery
Replacement“).
TO CONNECT THE ELECTRODE
With the meter turned off, connect the HI14143/50 probe
to the DIN socket on the bottom of the meter by aligning the
pins and pushing in the plug firmly. Remove the protective
cap from the probe before taking any measurements.
OPERATIONAL GUIDE

9
OPERATIONAL GUIDE
TO TURN THE METER ON
To turn the meter ON, press the button on
the front of the meter. If it does not turn on, make
sure that the batteries are properly installed in their place.
The meter is provided with an active acoustic signal when
a key is pressed. At start‑up the meter displays all LCD
segments for a few seconds, followed by the percentage
indication of the remaining battery life, the meter enters
the normal measurement mode.
Note: The meter detects the presence and the type of the
probe at its input.
- If the probe is not connected the message “NO”
”PROBE“ appears alternatively on the secondary LCD
with “---“ blinking on the first LCD line.
- If the probe is not compatible “WRONG” ”PROBE“
message appears alternatively on the secondary LCD
with “---“ blinking on the first LCD line.
- If the readings are out of range, the nearest range
limits are displayed blinking (E.g. -2.00 pH -5.0 °C).
TO SELECT THE MEASUREMENT RANGE
While in measurement mode, press the SET button to select
pH or pH‑mV measurement on the first LCD line.
Battery life
Measurement
mode
LCD
check up
→ →
→
Electrode
check

10
TO FREEZE THE MEASUREMENT VALUES
While in measurement mode, press and hold the SET
button until “HOLD“ appears on the secondary LCD. The
“HOLD“ remains for 1 second and reading of pH, mV
and temperature will be frozen on the LCD with“H” blinking.
Press any button to resume active measurements.
TO ENTER CALIBRATION MODE
Press and hold the button until ”POWER” and tag
is replaced by “STD“ and tag. Release the button.
TO ENTER SETUP MODE
Press and hold button until “STD“ and tag is
replaced by “SETUP“ and tag. Release the button.
TO TURN THE METER OFF
Whileinmeasurementmode,pressthe button.”POWER”
and tag will appear. Release the button.
pH MEASUREMENT & CALIBRATION
Make sure the meter has been calibrated before use.
If the probe is dry, soak it in HI70300 storage solution for
30 minutes to reactivate it. If fouled, clean the electrode
by soaking in cleaning solution for
20 minutes, then rinse the tip and
soak in storage solution at least
30 minutes before use. Rinse the
electrode off well and shake off
excess droplets. Recalibrate before using. Submerge
the probe in the sample to be tested while stirring
it gently. Wait until the tag on the LCD disappears.
The LCD displays the pH value (automatically compensated
for temperature) on the primary LCD, while the secondary
LCD displays the sample temperature.
OPERATIONAL GUIDE
→

11
If measurements are taken in different samples succes‑
sively, rinse the probe tip thoroughly†to eliminate cross‑
contamination.
For better accuracy, frequent calibration of the pH sensor
with the meter is recommended. In addition, the meter
must be recalibrated whenever:
a) The pH electrode is replaced.
b) After testing aggressive chemicals.
c) Where high accuracy is required.
d) At least once a month.
e) After cleaning the sensor.
†The probe tip should be rinsed with purified water (reverse
osmosis, distilled, or deionized) before and after placing in
any solution (buffer, storage, or sample).
pH calibration
Enter calibration mode while in pH
measurement mode. Place the sensor
into the first calibration buffer. If per‑
forming a two‑point calibration, use
pH 7.01 (pH 6.86 for NIST) buffer
first. The meter will enter the calibration mode, displaying
“pH 7.01 USE” and tag blinking (or “pH 6.86
USE” for NIST).
Follow directions for single and two‑point calibration below:
Single-point calibration
1. Place the probe in any buffer from the selected buffer
set. The meter will automatically recognize the buffer
value.
2. If the buffer is not recognized or the calibration offset is
out of the accepted range “---- WRONG“ is displayed.
3. If the buffer is recognized “REC“ is displayed then
“WAIT“ until the calibration is accepted.
If using pH 7.01 (or pH 6.86 for NIST), after acceptance
of the buffer press any key to exit. The ”SAVE” message is
displayed and the meter returns to pH measurement mode.
If using pH 4.01 or 10.01 (or pH 9.18 for NIST) buffer
the ”SAVE” message is displayed and meter returns to pH
measurement mode.
OPERATIONAL GUIDE

12
Two-point calibration
Proceed with steps 1 through 3 under single point
calibration using 7.01 (pH 6.86 for NIST) pH buffer first.
Then follow steps below:
The ”pH 4.01 USE” message is then displayed.
Place the probe in the second calibration buffer (pH 4.01
or 10.01, or, if using NIST, pH 4.01 or 9.18). When the
second buffer is accepted, the LCD will display “SAVE”
for 1 second and the meter will return to the normal
measurement mode.
If the buffer is not recognized or the slope is out of accepted
range ”--- WRONG” is displayed. Change the buffer, clean
the electrode or press any key to exit calibration.
It is always recommended to carry out a two‑point calibra‑
tion for better accuracy.
When the calibration procedure is completed, the tag
is turned on together with the calibrated points.
To exit calibration and reset default values
After entering the calibration mode and before the first point
is accepted, it is possible to quit the procedure and return
to the last calibration data by pressing the button. The
LCD displays “ESC“ for 1 second and the meter returns to
normal mode.
To reset the default values and clear a previous calibration,
press the SET button after entering the calibration mode
and before the first point is accepted.
The LCD displays “CLEAR“ for 1 second, the meter resets to
the default calibration and the tag with the calibrated
points on the LCD disappears.
OPERATIONAL GUIDE

13
OPERATIONAL GUIDE
ELECTRODE CONDITION
The display is provided with a probe icon (unless the fea‑
ture is disabled from setup) which indicates the electrode
status after calibration. The “condition” remains active for
12 hours (unless the batteries are removed).
The electrode condition is evaluated only if the current
calibration has two points.
5 bars: excellent condition
4 bars: very good condition
3 bars: good condition
2 bars: fair condition
1 bar: poor condition
1 bar blinking: very poor condition
With 1 bar it is recommended to clean the electrode and
recalibrate. If there is still only 1 bar or 1 bar blinking
replace the probe.
Sensor Check
Setting the meter to pH‑mV range the user can check the
sensor status at any time. The offset value is the reading
in pH 7.01 buffer (@ 25 °C/77 °F). If this reading is
outside the range ±30 mV, the electrode is considered
“very poor”. The slope value of the sensor is the difference
between readings in pH 7.01 and in pH 4.01 buffers. When
the slope reaches the value of about 150 mV, the electrode
is considered “very poor“. When “poor“ or “very poor“, it
is recommended to replace it with a new one.
Note: To ensure reliable readings, the electrode must
be cleaned with cleaning solution and then hydrated in
storage solution for a minimum of 30 minutes before
calibrating the probe.

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Setup mode allows the selection of the Temperature unit,
Auto‑off, Beep, the type of pH buffer set, the Resolution and
Information. To enter Setup mode press and hold button
until “STD“ and tag is replaced by “SETUP“ and
tag. Release the button.
• “TEMP“ is displayed on the second‑
ary LCD with the current temperature
unit (E.g. “TEMP °C“), for °C/°F
selection, use the SET button. Af‑
ter the temperature unit has been
selected, press to confirm and to enter the “A-OFF“
selection.
• Use the SET button, to cycle through
the auto‑off choices: 8 minutes (“8”,
default value), 60 minutes (“60”) or
disabled (“---”). Press to confirm
and to enter the “BEEP“ selection.
• To switch ON or OFF the beep tone,
press the SET button; press to
confirm and to enter the calibration
buffer selection “pH 7.01 BUFF“.
• To change the type of calibration
buffer set, the meter will show the
current buffer set: “pH 7.01 BUFF”
(for standard buffer set: 4.01/7.01/
10.01) or “pH 6.86 BUFF” (for
NIST buffer set: 4.01/6.86/ 9.18). Change the set with
the SET button. Press to confirm and to enter pH
resolution selection “RESOL“.
• To change the pH measurement
resolution between “0.1“ and “0.01“
use the SET button; then press to
confirmand toenterelectrodecalibra‑
tion information “INFO“ selection.
METER SETUP

15
METER SETUP
• To switch ON or OFF the electrode
condition indicator on the LCD, press
the SET button; press to exit
setup options; Change the set with
the SET button, then press to
confirm and to return to normal mode.
When the remaining battery life is less
than 10% the battery tag blinks on the
display to warn the user.
Battery Error Prevention System (BEPS)
If the battery is too weak (“0%”) the display shows
“bAtt“, “DEAD“ for few seconds then the meter power off.
Immediately replace the batteries with new ones.
The batteries are accessed by opening the battery cover
on the back of the instrument. Remove protective boot
if present.
BATTERY REPLACEMENT
BATTERY REPLACEMENT

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HI14143/50
Preamplified pH/temperature probe with
flat tip 50 mm‑long body, DIN connector
and 1 m (3.3’) cable
HI7004L pH 4.01 buffer solution, 500 mL
HI7006L pH 6.86 buffer solution, 500 mL
HI7007L pH 7.01 buffer solution, 500 mL
HI7009LpH 9.18 buffer solution, 500 mL
HI7010L pH 10.01 buffer solution, 500 mL
HI70300L pH electrode storage solution, 500 mL
HI7061L pH electrode cleaning solution, 500 mL
HI70620L Cleaning and disinfection solution for
skin residual, 500 mL
HI70621L Cleaning solution for skin grease and
sebum, 500 mL
HI710029 Silicon rubber boot blue color
HI76405 Electrode holder
HI77400P Calibration kit (pH 4 & 7, 20 mL, 5 pcs.
each)
ACCESSORIES BATTERY REPLACEMENT
Replace the three 1.5V AAA alkaline batteries located in
the battery compartment, observing the indicated polarity.
Replace the battery cover making sure that the gasket is
in place.

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ACCESSORIES
Code and serial
number on the
other side
Temperature sensor
junction reference
pH sensor
Quick connector with
Rubber protection, and
1 meter cable
ELECTRODE MAINTENANCE
PREPARATION
• Remove the protective cap. DO NOT BE ALARMED IF
ANY SALT DEPOSITS ARE PRESENT. Rinse with water.
• Shake the electrode down as you would do with a
clinical thermometer to eliminate any air bubbles inside
the glass.
• If the glass and/or junction are dry, soak the electrode in
HI70300 Storage Solution for a minimum of 30 minutes.
• Rinse with water.
• Calibrate before using.
STORAGE
• To ensure a quick response, the glass and the junction
should be kept moist and not allowed to dry.
• Replace protective cap with a few drops of HI70300
Storage Solution. Follow PREPARATION above before
taking measurements.
Note: NEVER STORE THE ELECTRODE IN DISTILLED WATER.

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MAN99181 05/18
All Hanna Instruments conform to the CE European Directives.
Disposal of Electrical & Electronic Equipment. The
product should not be treated as household waste. Instead
hand it over to the appropriate collection point for the
recycling of electrical and electronic equipment which will
conserve natural resources.
Disposal of waste batteries. This product contains
batteries, do not dispose of them with other household
waste. Hand them over to the appropriate collection point
for recycling.
Ensuring proper product and battery disposal prevents
potential negative consequences for the environment and
human health. For more information, contact your city,
your local household waste disposal service, the place
of purchase or go to www.hannainst.com.
RoHS
compliant
CERTIFICATION ELECTRODE MAINTENANCE
PERIODIC MAINTENANCE
• Inspect the electrode for any scratches or cracks. If any
present, replace the electrode.
• Rinse off any salt deposits with water.
• Follow the STORAGE procedure above.
CLEANING PROCEDURE
• Soak in Hanna HI7061 general cleaning solution,
HI700620 cleaning and disinfection solution for skin
residual or HI700621 cleaning solution for skin grease
and sebum for 20 minutes. Rinse with water.
IMPORTANT: After performing any of the cleaning
procedures rinse the electrode thoroughly with distilled
water. Soak the electrode in HI70300 Storage Solution
for a minimum of 30 minutes. Rinse with water. Calibrate
before using.
TROUBLESHOOTING
• pH Meter: Follow the meter’s operating and calibration
procedures.
• Electrode: Evaluate your electrode performance based on
the SENSOR CHECK procedure on page 13.

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HI99181 is warranted for two years against
defects in workmanship and materials when
used for its intended purpose and maintained
according to instructions. This warranty is
limited to repair or replacement free of
charge. Damage due to accidents, misuse,
tampering or lack of prescribed maintenance
is not covered.
If service is required, contact your local Hanna
Instruments Office. If under warranty, report
the model number, date of purchase, serial
number (see engraved in the back of the
meter) and the nature of the problem. If the
repair is not covered by the warranty, you will
be notified of the charges incurred. If the meter
is to be returned to Hanna Instruments, first
obtain a Returned Goods Authorization number
from the Technical Service department and
then send it with shipping costs prepaid. When
shipping any meter, make sure it is properly
packed for complete protection.
Warranty
Hanna Instruments reserves the right to modify the design,
construction or appearance of its products without advance notice.
Before using this product, make
sure it is entirely suitable for your
specific application and for the en‑
vironment in which it is used. Any
variation introduced by the user
to the supplied equipment may
degrade the meters’ performance.
For yours and the meter’s safety
do not use or store the meter in
hazardous environments.
Recommendations
for Users

Printed in ROMANIA
World
Headquarters
Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
www.hannainst.com
MAN99181
Local Office
Hanna Instruments Inc.
270 George Washington Highway
Smithfield, RI 02917
Phone: [phone]
Fax: [phone]
e‑mail: [email protected]