PermkinElmer NexION 300 ICP-MS User manual

Introduction
PerkinElmer®ICP-MS instruments are complete systems
with the exception of the following items which must
be provided by the customer: electrical power, exhaust
vents, argon gas supplies with approved regulator, cell
gas supply, and coolant system.
Required Environmental Conditions
The laboratory environment in which the NexION®300
ICP-MS instrument is installed should meet the following
conditions:
• Theroomtemperatureshouldbebetween15and
30˚C(59-86˚F)withamaximumrateofchangeof
3˚C(5˚F)perhour.
• Therelativehumidityshouldbebetween20and
80%,non-condensing.Foroptimumperformance,
the room temperature should be controlled at
20±2˚C(68±3.6˚F),andtherelativehumidity
shouldbebetween35and50%.
• Theinstrumentiscertifiedforoperationatelevationsup
to2000meters(6562ft.)abovesealevel.
In addition, the instrument should be located in an
area that is:
- Indoors
-Freeofsmoke,dustandcorrosivefumes
- Not prone to excessive vibration
- Out of direct sunlight
- Away from heat radiators
In order to minimize contamination problems, a
dust-freeenvironmentisnecessary.Forultra-trace
techniques, environmental contamination becomes a
limiting factor in the analysis. To quantitate ubiquitous
elementssuchasFe,Ca,K,Na,etc.below1ppb(µg/L),
aclass1000environmentisnecessaryforsample
preparation and analysis. This is not an indication of
the performance limitations of the instrument, but
a recommendation for an ultra-clean environment.
The NexION 300 ICP-MS can be installed into a mobile
laboratory if vibration is isolated.
PREPARING YOUR LAB
ICP-Mass Spectrometry
Preparation Checklist
• Environmentalconditions
• Electricalrequirements
• Spacerequirements
• Exhaustventilation
• Coolantrequirements
• Argongasrequirements
• Cellgasrequirements
NexION300ICP-MS

2
• Wearappropriateeyeprotectionatalltimeswhile
handling chemicals. Depending on the types of chemicals
you are handling, wear safety glasses with side shields, or
goggles, or a full-face shield.
• Wearsuitableprotectiveclothing,includingglovesif
necessary, resistant to the chemicals you are handling.
• Whenpreparingchemicalsolutions,alwaysworkinafume
hood that is suitable for the chemicals you are using.
• Performsamplepreparationawayfromtheinstrument
to minimize corrosion and contamination.
• Cleanupspillsimmediatelyusingtheappropriateequipment
and supplies, such as spill-cleanup kits.
Location and Space Requirements
Space Requirements
The system should be located near the required electrical
and gas supplies as well as the coolant supply. The roughing
pumpcanbelocateduptoadistanceof2meters(6.5ft.)
fromtheinstrument–upto3meters(10ft.)usingoptional
kit.Therecanbenomorethan2to3bendsorcouplingsin
the vacuum hose over its entire length. The diameter of the
hosemustremainatleast25mm(1in.)ID.
The NexION 300 ICP-MS is designed to operate on a bench
66-91cmhigh(26-36in.high).PerkinElmeroffersabench
designedfortheNexION300ICP-MS(PartNo.N8141230).
Thisbenchis76cmwidex152cm(198cmwithshelf
extended)longx74cmhigh(30in.widex60in.long
[78in.withshelfextended]x29in.high).Thisbenchhas
storage bins and an acoustic barrier to muffle the sound of
the roughing pump.
Allow space on the right and left sides of the instrument for
the workstation or any accessories. The main air intake is
on the left-hand side of the instrument and a minimum of
45cm(18in.)clearanceisrequired.Inoperation,NexION
can be operated with the back up against a wall. Access for
most service procedures is through the front of the instrument.
However, some infrequent service procedures may require a
spaceofatleast30cm(12in.)behindtheinstrument.
Storage Conditions
• Ambienttemperature:–20˚Cto+60˚C(–4˚Fto+140˚F).
• Relativehumidity20%to80%,withoutcondensation.
• Altitude:intherange0mto12,000m(sealevelto
39,370ft.).
NOTE: Whenyouremovetheinstrumentfromstorageandbeforeyouputit
intooperation,allowittostandforatleastadayundertherequired
environmentalconditions.
General Laboratory Requirements
Laboratory Hygiene
• Keeptheworkareascrupulouslycleantoavoidcon-
taminating your samples and to maintain a safe working
environment. Clean up spilled chemicals immediately and
dispose of them properly.
• Donotallowwastetoaccumulateintheworkarea.
Dispose of waste correctly.
• Donotallowsmokingintheworkarea.Smokingisa
source of significant contamination and also a potential
route for ingesting harmful chemicals.
• Donotstore,handle,orconsumefoodintheworkarea.
• Ensurethattheareaaround,underandbehindthe
instrument is clear of any dirt and dust to prevent their
entry into the instrument’s interior, which could cause a
negative effect on performance.
Working with Chemicals
Some chemicals used with the instrument may be hazardous
or may become hazardous after completion of an analysis.
• Use,store,anddisposeofchemicalsinaccordancewith
the manufacturer’s recommendations and the applicable
national,state,and/orlocalregulations.
• DoNOT put open containers of solvent near the instrument.
• Storesolventsinanapprovedcabinet(withtheappropriate
ventilation)awayfromtheinstrument.
EXPLOSIVE ATMOSPHERE. This instrument is not
designed for operation in an explosive atmosphere.
!
WARNING

3
Table 1. Dimensions of the Instrument and Computer.
Instrument Width Height Depth Weight
cm (in.) cm (in.) cm (in.) kg (lb.)
NexION 122.5* 76 75 181
ICP-MS (49)* (30.5) (30) (400)
Computer Dimensionswillvarybymodel
Monitor Dimensionswillvarybymodel
Printer Dimensionswillvarybymodel
*Width including the shipping handles is 141 cm (56.5 in.)
The NexION 300 ICP-MS can be positioned in either a linear
oranL-shapedconfiguration.IntheL-shapedconfiguration,
the computer and printer are positioned on one leg of the
L.Theinstrumentandanaccessorytablemakeuptheother
leg.ArecommendedworkstationlayoutisshowninFigure2.
There should be sufficient space near the spectrometer for
thevariousaccessories(autosampler,laseretc.).Itis
recommended that the accessories be placed on a movable
cart or table to allow for easy service access.
The system computer may be placed on the instrument
bench or a separate computer table.
System Layout
The ICP-MS system consists of the main instrument, roughing
pump, the computer controller assembly, and a printer. The
dimensionsoftheinstrumentaregiveninFigure1.Table1lists
thedimensionsoftheinstrumentandthecomputer.Table2
lists the dimensions of the peripherals and accessories.
Figure 1.DimensionsofNexION300ICP-MSspectrometer.
Figure 2.Recommendedworkstationlayout.
Table 2. Dimensions of the Peripherals and Accessories.
Peripherals Width Height Depth Weight
cm (in.) cm (in.) cm (in.) kg (lb.)
VacuumRoughingPump 50(20) 30(12) 30(12) 45(100)
CoolingSystem
HeatExchanger 56(22) 57(22.5) 38(15) 37.6(83)
(PolyScience®3370)
RefrigeratedChiller 36.5(14) 61(24) 67.3(26.5) 86(189)
(PolyScience®6106PE) 
Accessory
S10Autosampler 44(17) 37(15) 34(14) 4(9)
75cm(30in.)
122.5cm(49in.)
76cm
(30.5in.)
66-91cm
(26-36in.)
32cm
max.
(12.6in.)
94-119cm
(37-47in.)

4
Drain Vessels
A drain vessel is supplied with the NexION 300 ICP-MS. The
vesselismadeofHDPE(highdensitypolyethylene)andis
used to collect the effluent from the ICP sample-introduction
system. The NexION 300 ICP-MS also has a torch box drain
with a drain line and a small waste bottle. Any waste accu-
mulated in either of these bottles should be disposed of in
compliance with your local environmental regulations.
The drain vessel should be placed to the right of the instrument.
The drain vessel should NOT be stored in an enclosed storage
area. The drain system should be checked regularly and
replaced when necessary. Should it become necessary to
replace the drain vessel, it should be made from a material
not likely to be attacked by samples being analyzed. Glass
or other brittle materials must NOT be used.
Liquidwasteshouldalwaysbesegregatedandclearlylabeled.
Never mix organic and inorganic liquids in the same drain
vessel. Organic and inorganic drain vessels should never be
stored in the same area.
Connections
Illustrated below are the connection locations and lengths.
Safe Handling of Gas Cylinders
NOTE:Thepermanentinstallationofgassuppliesistheresponsibilityofthe
userandshouldconformtolocalsafetyandbuildingcodes.
• Fastenallgascylinderssecurelytoanimmovablebulkhead
or a permanent wall.
• Whengascylindersarestoredinconfinedareas,suchas
a room, ventilation should be adequate to prevent toxic
or explosive accumulations. Move or store gas cylinders
only in a vertical position with the valve cap in place.
• Locategascylindersawayfromheatorignitionsources,
including heat lamps. Cylinders have a pressure-relief
device that will release the contents of the cylinder if the
temperatureexceeds52˚C(125˚F).
• Whenstoringcylindersexternaltoabuilding,thecylinders
should be stored so that they are protected against tem-
peratureextremes(includingthedirectraysofthesun)
and should be stored above ground on a suitable floor.
• Markgascylindersclearlytoidentifythecontentsand
status(full,empty,etc.).
• DoNOT attempt to refill gas cylinders yourself.
Figure 3.Locationandlengthofconnections.
*Cellgastubingis3meterslong(10ft.)stainlesssteel.Twoorthreepiecessupplieddependinguponinstrumentmodel.Additionalcellgastubing3m
(10ft.)canbeorderedusing(PartNo.N8141269).
**Ifusingtheoptionalremotecontrolkit(PartNo.N8140505),theACpowercord(withadaptercable)andtheremotecontrolcableareeach213cm
(7ft.)long.
+Optional3mvacuumhose(PartNo.WE024084)isavailableifneeded.
Twoexhausthoseslength
is3m(10ft.)each
Ethernetcablelength
is3m(10ft.)
Computer
powercord
lengthis
183cm
(6ft.)
NexION300ICP-MSpower
cordlengthis245cm(8ft.)
Exhausthoselength
is3m(10ft.)
Pumppowercord
lengthis2m(6.5ft.)
Chiller/Heat
Exchangerpower
cordlengthis2m
(6.6ft.)
Cellgaslinelength
is3m(10ft.)each*
UniversalCellTechnology
((UCT)instrumentsonly)
Argongastubing
lengthis3m(10ft.)
[PartNo.09985715]
Coolanthose
lengthis3m(10ft.)
Pumpcontrolcable
lengthis3m(10ft.)
Vacuumhoselengthis2m(6.5ft.)+

Electrical Requirements
Power to the NexION 300 ICP-MS is to be delivered from
one30A/32Asingle-phase200-240Vdedicatedelectrical
branchcircuit,accordingtothepowerspecificationsinTable4.
Table5providestheelectricalsupplyrequirementsand
approximate power consumption of the peripherals. If the
power line is unstable, fluctuates or is subject to surges,
additional control of the incoming power may be required.
The vacuum roughing pump is provided with a mains supply
plugsuitableforthecountryofinstallation(showninFigure4)
andmustbeconnectedtoaseparatebranchcircuit/wall
outlet.Itrequiresone15Asingle-phase200-240Voutlet
–seeTable5.SeeFigure3(Page4)forthelocationand
lengths of hoses, lines, cords and cables.
Facilities Requirements
Table3providesinformationonthegasandliquidservicesrequiredfortheNexION300ICP-MS.Tables4and5showthe
electrical supply requirements and approximate power consumption of the NexION 300 ICP-MS and its major accessories.
Table 3. Gas and Liquid Services Required for the NexION 300 ICP-MS.
Gases Operating pressure Flow at operating pressure
Argon 586-690kPa(85–100psig)min-max 14-20L/min(typical)
Purityseepage8   
Ammonia≥99.999%pure 48±14kPa(7±2psig)operating 0.6mL/min(typical)
(forUCTinstrumentsonly)   
Helium≥99.9999%pure 290±14kPa(42±2psig)operating 5mL/min(typical)
(forUCTinstrumentsonly)    
Cooling Liquid 344±14kPa(60±2psig) 3.8L/min(1.0gpm)minimum
  4.7L/min(1.25gpm)typical
5
Table 4. NexION 300 ICP-MS Power Specifications.
Power Consumption:
MaximumVoltAmperes(total) 3720VA
MaximumContinuousCurrent 22A
Voltage Amplitude Specification:
OperatingVoltage 200-240
AllowableVoltageVariance ±10%
MaximumAllowablePercentSag 5%
MaximumAllowablePercentSwell 5%
Phase Singlephaseonly
Frequency Specification:
OperatingFrequency 50/60Hz
AllowableFrequencyVariance ±1Hz
Waveform Specification:
MaximumSupplyVoltage
TotalDistortion 5%
MaximumSupplyVoltage
DistortionbySingleHarmonic 3%
Figure 4. Vacuumroughingpumpmainssupplyplugs.
North America
Japan
NEMA 6-15P
N8145006
Europe
CEE 7
“Schuko”
N8145007
Switzerland
N8145009
United Kingdom
BS 1363
N8145008
Rest of World
No plug
N8145010
MAGNETIC SUSCEPTIBILITY. Do NOT place NexION
300 ICP-MS close to any other instrumentation
or equipment that emits high magnetic fields.
External magnetic field strength must not exceed
10 Gauss at NexION 300 ICP-MS.
• Useonlyapprovedregulatorsandhoseconnectors.Left-
hand thread fittings are used for fuel gas tank connections
whereas right-hand fittings are used for oxidant and
support gas connections.
• Arrangegashoseswheretheywillnotbedamagedor
stepped on and where things will not be dropped on
them.
• Performperiodicgasleaktestsbyapplyingasoapsolution
to all joints and seals.
• ItisstronglyrecommendedthatUniversalCellTech-
nology™(UCT)gasesbeinstalledinagascabinetwith
adequate ventilation.
!
WARNING

Exhaust and Ventilation Requirements
The NexION 300 ICP-MS has two separate exhaust ports.
Both of the NexION 300 ICP-MS exhaust ports are located
onthetopoftheinstrument(seeFigure6).Thetorchbox
exhaustportislocated46.5cm(18.5in.)fromtherightside
oftheinstrument.TheRFgeneratorexhaustportislocated
67.5cm(27in.)fromtheleftsideoftheinstrument.
The torch box exhaust port exhausts the following:
• Plasmaheatandfumes
• Vacuumpumpexhaust–includingcellgasesonUniversal
Cell Technology™(UCT)instruments
• Cellgasassemblymanualvent/purgeswitch
The torch box exhaust venting system is required to remove
combustion fumes and vapors from the torch housing, and
to remove reaction cell gas. Exhaust venting is important for
four reasons:
• Itprotectslaboratorypersonnelfromtoxicvaporsthat
may be produced by some samples.
• Itminimizestheeffectsofroomdraftsandthelaboratory
atmosphere on ICP torch stability.
• Ithelpsprotecttheinstrumentfromcorrosivevapors
which may originate from the samples.
• ItremovesdissipatedheatwhichisproducedbytheICPtorch.
Thetorchexhaustportalwayshas0.5inchesofwater(125Pa)
staticpressure.TheRFgeneratorexhaustporthas0.5inches
ofwater(125Pa)staticpressurewhentheplasmaisoff–
during operation, the static pressure drops to zero. Both of
the exhaust ports should be connected directly to flexible
exhausthoses.Usetheventadaptertoattachtheroughing
pump exhaust hose to the torch box exhaust port.
Table 5. Electrical Requirements for NexION 300 ICP-MS
Peripherals.
Equipment Voltage(AC) Power
Computer 100-127V/200-240V, 800W
 50/60Hz typical
Printer 100-127V/220-240V, 800W
 50/60Hz typical
RoughingPump 200-240V,50/60Hz12A 1500W
CoolingSystem:
HeatExchanger 208-240V,50/60Hz 660W
(PolyScience®3370) 120V,60Hz5.5A
or
Refrigeratedchiller 208-230V,60Hz12.2A 2500W
(PolyScience®6106PE) 240V,50Hz12.2A
Mains Connection
Theinstrumentisshippedwithone2.4m(8ft.)mainscord
terminatedbyanIEC60309connectorrated30AbyUL(North
America)and32AbyVDE(International)for250Vasshownin
Figure5.
6
Figure 5.IEC60309connector.
Figure 6. Locationofexhaustports.
EXPLOSIVE ATMOSPHERE. The use of ICP-MS
instruments without adequate ventilation to
outside air may constitute a health hazard.
For example, the combustion of halogenated
hydrocarbons produces toxic vapors. When
ammonia is used as a Dynamic Reaction Cell
gas, it can be present at very trace amounts
in the exhaust during purging of the gas lines.
Extreme care should be taken that exhaust
gases are vented properly.
!
WARNING
67.5cm
(27in.)
46.5cm
(18.5in.)
RFGenerator
ExhaustPort
TorchBox
ExhaustPort

Thetorchboxexhaustmustbeinstalled,buttheRFgenerator
exhaustisoptional(seenoteinTable7*).
TheRFgeneratorexhaustventisusedfortheblowerthat
cools the system power supplies and ICP generator.
Werecommendusingtwo100-mm(4in.)exhausthoses
(forboththetorchboxexhaustventandRFgenerator
exhaustvent).TheNexION300ICP-MSissuppliedwith6m
(22ft.)of100-mm(4in.)flexibletubing.Thistubingpermits
the movement of the instrument without disconnecting the
ventsfromthelaboratorysystem.SeeTables6and7for
vent specifications.
Inoperation,theroughingpumpproduces1200-1500W
(4100-5100BTU/hr)ofheat.Theheatfromtheroughing
pump is released into the laboratory. Proper ventilation is
required to remove this heat from the room or any enclosure
in which the pump is situated. There must be a minimum of
15cm(6in.)clearancebetweentherearofthepumpand
anyverticalsurfaceaswellasaminimumof35cm(14in.)
clearance in the front. It should be located away from other
heat generating sources such as the liquid cooling system.
TheambientairtemperaturemustNOTexceed40°Catthe
roughing pump control electronics.
Theheatfromtheliquidcoolingsystem(heatexchangeror
refrigeratedchiller)isalsoreleasedintothelaboratoryduring
operation. The heat exchanger will produce a maximum of
2000W(6825BTU/hr)ofheat.Therefrigeratedchillerwill
produceamaximumof3000W(10,000BTU/hr)ofheat.
Proper ventilation is required to remove this heat from the
room or any enclosure in which the liquid cooling system
is situated. Adequate clearance should be allowed on the
front, sides, and rear of the unit for access to connections
and components. The front and rear vents of the unit must
beaminimumof61cm(2ft.)awayfromwallsorvertical
surfaces so air flow is not restricted. It should be installed at
7
least1.4meters(4ft.)awayfromanyheat-generating
sources such as the roughing pump or other instruments.
Proper ventilation is critical for the heat exchanger – its
ambient air temperature must never exceed 30 ˚C.
Venting System Recommendations
Theexhaustflowrateattheinstrument(theabilitytovent
thesystem)isdependentoncustomer-providedblower,the
duct length, material and the number of elbows or bends
used. If an excessively long duct system or a system with
many bends is used, a stronger blower may be necessary to
provide sufficient exhaust volume at the instrument. Smooth
stainless steel tubing should be used instead of flexible stain-
less steel tubing, where flexibility is not required, to reduce
system friction loss or “drag.” A length of smooth stainless
steelductinghas20-30%lessfrictionlossthanacomparable
lengthofflexibleducting.Whensmoothstainlesssteel
tubing is used, elbows must be used to turn corners. These
elbowsshouldturnatnomorethan45degreesbetween
straight sections to reduce friction losses, and the number
of elbows should be minimized.
Additional recommendations on the venting system include:
• Theductcasingandventingsystemshouldbemadeof
materialssuitablefortemperaturesashighas70˚Cand
be installed to meet local building code requirements.
• Locatetheblowerasclosetothedischargeoutletaspossible.
All joints on the discharge side should be airtight, especially if
toxic vapors are being carried.
• Equiptheoutletendofthesystemwithabackdraft
damper and take the necessary precautions to keep the
exhaust outlet away from open windows or inlet vents
and to extend it above the roof of the building for proper
dispersal of the exhaust.
• Equiptheexhaustendofthesystemwithanexhaust
stack to improve the overall efficiency of the system.
Table 7. Hose Diameter and Venting Capabilities.*
Hose Hose Diameter Heat Vented Outside Lab Watts (BTU/hr)
TorchBoxExhaust 100mm(4in.) 800(2720)
RFGeneratorExhaust 100mm(4in.) 1500(5100)
*IfonlytheTorchBoxexhausthoseisused,approximately1500W(5100BTU/hr)ofheatfromtheRFgeneratorexhaustisventedintothelab.
Anindependentroomairconditioner[3000W(10000BTU/hr)]isrecommendedtoremovethisadditionalheat.
Table 6. Instrument Exhaust Ventilation Requirements.
Required airflow Required air velocity Reference airflow Reference air velocity
measured with hose connected to NexION measured with hose disconnected from NexION
TorchBox
ExhaustPort 73–100ft3/min@0.5"H2O 836–1145ft/min@0.5"H2O 110–150cfm@0"H2O 1260–1719ft/min@0"H2O
(35–47L/sec@125Pa) (4.3–5.8m/sec@125Pa) (52–71L/sec@0Pa) (6.4–8.7m/sec@0Pa)
RFGenerator
ExhaustPort 150–160ft3/min@0"H2O 1719–1833ft/min@0"H2O 150–160ft3/min@0"H2O 1719–1833ft/min@0"H2O
(71–76L/sec@0Pa) (8.7–9.3m/sec@0Pa) (71–76L/sec@0Pa) (8.7–9.3m/sec@0Pa)

Gas Requirements
Argon Gas Requirements
Argon is used as the ICP torch gas with the NexION 300
ICP-MS. The argon-purity criteria are listed below.
Purity ≥99.996%
Oxygen <5ppm
Hydrogen <1ppm
Nitrogen <20ppm
Water <4ppm
It is also important to note that the amount of krypton
impurity in the argon gas will negatively affect the ability
of the instrument to quantitate selenium. The best selenium
detectionlimitsareachievedwhenkrypton<0.1ppb
(0.0001ppm).
Either liquid or gaseous argon can be used with an ICP-MS
system. The choice of liquid argon or gaseous argon tanks
is determined primarily by the availability of each and the
usagerate.Liquidargonisusuallylessexpensiveperunit
volume to purchase, but cannot be stored for extended periods.
If liquid argon is used, the tank should be fitted with an
over-pressure regulator which will vent the tank as necessary
in order to prevent the tank from becoming a safety hazard.
Gaseous argon tanks do not require venting and consequently
can be stored for extended periods without loss. A tank of
liquidargon,whichwillproduce4300ft3of argon gas, will
lastforapproximately100hoursofcontinuousICPrunning
time.Atankofgaseousargonwilllast5to6hoursofICP
runningtime.Thenormalargongasusageis14-20L/min.
Acylinderregulator(PartNo.03030284)whichcanbeused
with argon is available from PerkinElmer. The regulator can
beusedwithCGA580orCGA590fittingsandincludesa
color-codedhosewith1/4-inchSwagelok®fittings to permit
direct connection to the regulator and to the instrument gas
controls.Liquidargonmaybepurchasedfromyourgassupplier.
PerkinElmerICP-MSinstrumentsinclude3meters(10ft.)of
the tubing necessary for connecting argon to the instrument
(PartNo.09985715).
Cell Gas Requirements
ForNexION300ICP-MSsystemsequippedwithUniversal
CellTechnology(300X,300D,300S),thecustomeris
requiredtosupplythereactionand/orcollisiongas(also
referredtoascellgas)forintroductionintotheuniversalcell.
The type of gas used varies with the customer application,
but the most common cell gases used with the NexION 300
ICP-MS are ultra pure helium and anhydrous ammonia.
• Forbestefficiency,makesurethelengthoftheduct
that enters into the blower is a straight length at least
ten times the duct diameter. An elbow entrance into the
blower inlet causes a loss in efficiency.
• Providemake-upairinthesamequantityasisexhausted
by the system. An airtight lab causes an efficiency loss in
the exhaust system.
• Ensurethatthesystemisdrawingproperlybyplacinga
piece of cardboard over the mouth of the vent.
• Equiptheblowerwithanindicatorlightlocatednearthe
instrument to indicate to the operator when the blower is on.
Cleaning the Instrument
Before using any cleaning or decontamination methods,
except those specified by the manufacturer, users should
check with the manufacturer that the proposed method
will not damage the equipment.
Cleaning procedures can be found in the NexION 300
ICP-MS Maintenance Guide.
Coolant Requirements
The NexION 300 ICP-MS system requires a regulated source
offilteredcoolant.PerkinElmercoolant(PartNo.WE016558)
must be used on the NexION 300 ICP-MS instrument. The
recirculatoroperatingpressureshouldbe413±13kPa
(60±2psi).Acoolantflowofatleast3.8L/min(1.0gpm)
is required.
A cooling fluid containing a corrosion inhibitor is specified
to protect the aluminum components of the cooling system
andtheinterface.Tenlitersofpre-mixedcoolant(PartNo.
WE016558)issuppliedfortheheatexchangerorrefrigerated
chiller. If the air temperature at the liquid cooling system
isalwaysbelow30˚C(86˚F),theheatexchangercanbe
used in place of a refrigerated chiller. The heat exchanger
must be located in a well-ventilated area where the air
temperaturewillnotexceed30˚C(86˚F).See“Exhaust
andVentilation”sectionfordetails.Forlaboratorieswhere
theairtemperaturecanexceed30˚C(86˚F)atthecooling
system, a heat exchanger cannot be used and a refrigerated
chiller is required.
For60Hzinstallations,theM3370heatexchangerscome
witheitherNEMAL6-15P(230V)orNEMAL5-15P(120V)
connectors. The refrigerated chiller comes with a NEMA
L6-15Pconnector.
For50Hzinstallations,therefrigeratedchillercomeswitha
CEE 7 connector. The M3370 heat exchanger comes with a
NEMAL6-15Pconnector.
8

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The cell gases used by the universal cell must meet the
specificationsasshowninTable9.Thepurityofheliumentering
theinstrumentmustbe≥99.9999%pure.Thiscanbe
accomplished by using a gas cylinder with a built-in purifier,
orbyusing≥99.999%pureheliumcylindertogetherwith
the special gas purifier provided with each instrument.
AdedicatedUHPheliumcylinderisrequired;househelium
supplies must not be used. The purity of any other cell gas,
mustbe≥99.999%pure.
Theammoniagasisconsumedatatypicalrateof0.6mL/min;
therefore,onlyaverysmallcylinder(60L,2ft3)ofgasis
required. Cylinders should be secured upright in a ventilated
enclosuresuchasacabinetorfumehood.Foradditional
typesofcellgasesnotlistedinTable8,thecustomermust
purchaseaUHPdouble-stageregulatorcapableofsupplying
upto7mL/minat48kPa(7psig).Asuitabledouble-stage
regulator with the correct cylinder fittings can be purchased
from your local gas supplier
Depending upon the NexION 300 ICP-MS model, PerkinElmer
providesthepressureregulator(s),gasdeliverytubing,and
purifierforusewithUHPheliumand/orUHPanhydrous
ammonia. The pressure regulators are capable of supplying
thecellgasesattheworkingpressureslistedinTable8.
The NexION 300 ICP-MS is shipped with the regulator-
to-cylinderfittingsshowninTable8.Thecellgascylinders
should use this type of fitting when ordered from your local
gas supplier. The cleanliness of the cell gas lines is critical
for analytical performance. There should be no additional
fittings between the regulator, purifier, and the instrument.
The NexION 300 ICP-MS requires specially cleaned stainless
steelcellgaslines(included).Additional3mlengthsof
specially cleaned cell gas tubing are available from PerkinElmer
(PartNo.N8141269).
Table 8. Cell Gas Requirements for Instruments Equipped with Universal Cell Technology.
NexION Cell Gas Regulator- Cell Gases Used Operating Flow
300 ICP-MS Regulator Supplied to-Cylinder at Installation Rate and Pressure
Model Connection
300Q N/A N/A N/A N/A
300X UHPdualstageforHe(N8145021) CGA580 UHPHelium≥99.9999%pure 5mL/min@275kPa(40psig)
300D UHPdualstageforHe(N8145021) CGA580 UHPHelium≥99.9999%pure 5mL/min@275kPa(40psig)
 UHPdualstageforNH3(N8122255) CGA660 UHPNH3≥99.999%pure 0.6mL/min@48kPa(7psig)
300S UHPdualstageforHe(N8145021) CGA580 UHPHelium≥99.9999%pure 5mL/min@275kPa(40psig)
 UHPdualstageforNH3(N8122255) CGA660 UHPNH3≥99.999%pure 0.6mL/min@48kPa(7psig)
Table 9. Cell Gas Purity Requirements.
Gas Purity Grade Impurity Specification Notes
Helium ≥99.9999% O2 <0.01ppm ThisgradeofgascanbeinputdirectlyintotheNexIONICP-MS.
H2O <0.02ppm Externalpurifiernotrequired.
  THC <0.1ppm
N2 <5ppm
Helium ≥99.999% O2 <4ppm Thisgradeofgasrequirestheuseofanexternalgaspurifier(supplied).
H2O <5ppm
  THC <0.5ppm
N2 <8ppm
Ammonia ≥99.999% O2 <4ppm ThisgradeofgascanbeinputdirectlyintotheNexIONICP-MS.
H2O <2ppm
  THC <3ppm
N2 <6ppm