Phcbi MCO-170AC User manual

MCO-170AC
Please read the operating instructions carefully before using this product, and keep the operating
instructions for future use.
See page 78 for model number.
Operating Instructions
CO2 Incubator

2
CONTENTS
INTRODUCTION .......................................................................................... 4
INTENDED USE AND PRECAUTIONS ........................................................ 4
PRECAUTIONS FOR SAFE OPERATION ................................................... 5
LABELS ON INCUBATOR .......................................................................... 10
SYMBOLS ON INCUBATOR ..................................................................... 11
ENVIRONMENTAL CONDITIONS .............................................................. 11
INCUBATOR COMPONENTS
Unit .......................................................................................................... 12
Control panel ........................................................................................... 14
Remote alarm terminal ............................................................................ 15
INSTALLATION
Installation site ......................................................................................... 16
Installation ............................................................................................... 17
Connecting a CO2 gas cylinder ............................................................... 19
BEFORE COMMENCING OPERATION
Initial cleaning method............................................................................. 20
Removing inner attachments .................................................................. 21
Installing inner attachments ..................................................................... 23
Filling the humidifying pan ....................................................................... 24
FOR OPTIMAL CULTIVATION
Precautions for cultures........................................................................... 25
Preventing contamination ........................................................................ 26
CORRECT OPERATION ............................................................................ 27
HOME SCREEN ......................................................................................... 28
MENU SCREEN ......................................................................................... 30
BASIC PARAMETERS
Setting temperature .................................................................................. 32
Setting CO2 gas ....................................................................................... 33
Setting CO2 density ................................................................................. 34
Setting high limit temperature alarm ....................................................... 35
OPERATION/ALARM LOG
Displaying operation log .......................................................................... 36
Exporting operation log ............................................................................ 39
Setting log interval ................................................................................... 41
Setting Unique ID .................................................................................... 42
Displaying alarm log ................................................................................ 43
Exporting alarm log ................................................................................. 44
KEY LOCK FUNCTION
Setting key lock ........................................................................................ 46
Removing key lock................................................................................... 47

3
CONTENTS
ALARM PARAMETERS
Setting automatic set temperature alarm ................................................. 48
Setting automatic set temperature alarm delay ....................................... 49
Setting automatic set CO2 density alarm ................................................. 50
Setting automatic set CO2 density alarm delay ....................................... 51
Setting door alarm delay .......................................................................... 52
Setting ring back delay ............................................................................ 53
Setting remote alarm ............................................................................... 54
UV LAMP (OPTION)
Using UV lamp ........................................................................................ 55
Setting UV lamp ON period ..................................................................... 56
UV lamp life indication ............................................................................. 57
Setting UV timer extension ...................................................................... 58
Lighting the UV lamp for 24 hours ........................................................... 59
GAS AUTO-CHANGER (OPTION)
Connecting CO2 gas cylinders ................................................................ 60
Automatic CO2 gas supply line changeover ............................................ 60
Manual CO2 gas supply line A/B changeover ......................................... 62
OTHER PARAMETERS
Setting date display (format) .................................................................... 63
Setting date .............................................................................................. 64
Setting time ............................................................................................. 65
Setting keypad click ................................................................................. 66
ALARMS, SAFETY, AND SELF-DIAGNOSIS ............................................. 67
TROUBLESHOOTING ................................................................................ 70
DISPOSAL OF UNIT ................................................................................... 72
SPECIFICATIONS ...................................................................................... 77
PERFORMANCE ........................................................................................ 78
EMC PERFORMANCE ............................................................................... 78
SAFETY CHECK SHEET ........................................................................... 79

4
INTRODUCTION
■ Read the operating instructions carefully before using the product and follow the instructions for safe
operation.
■ PHC Corporation takes no responsibility for safety if the product is not used as intended or is used with
any procedures other than those given in the operating instructions.
■ Keep the operating instructions in a suitable place so that they can be referred to as necessary.
■ The operating instructions are subject to change without notice for improvement of performance or
function.
■ Contact our sales representative or agent if any page of the operating instructions is lost or the page
order is incorrect, or if the instructions are unclear or inaccurate.
■ No part of the operating instructions may be reproduced in any form without the express written
permission of PHC Corporation.
IMPORTANT NOTICE
PHC Corporation guarantees this product under certain warranty conditions. However, please note that
PHC Corporation shall not be responsible for any loss or damage to the contents of the product.
INTENDED USE AND PRECAUTIONS
This equipment is designed for culture of cells, tissues, organs, and embryos.
■ The adapted culture condition depends on the kind of sample. It is necessary to determine the culture
temperature, CO2 density, and culture period that is required.
■ For culture of embryos, low O2 density is desirable. Using an O2/CO2 incubator is recommended.
■ For IVF/ART use, special attention should be paid to traceability, since incidents may be disclosed
several months or years later, at the baby’s birth, or during its later life. It is therefore recommended that
the following data is maintained: product serial number, incubation term, and incubation parameters.
(Refer to details on MEDDEV 2.2/4.)

5
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with the operating instructions
as they contain important safety advice.
Items and procedures are described so that this unit can be used correctly and safely.
Following these precautions will prevent possible injury to the user and any other person.
Precautions are illustrated in the following way:
WARNING
Warning indicates a potentially hazardous situation which, if not avoided,
could result in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbols have the following meaning;
This symbol means caution.
T
his symbol means an action is prohibited.
T
his symbol means an instruction must be followed.
WARNING
As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity
of the equipment. It is important that you assess the work site to ensure there is suitable and
sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a
safe environment must be considered. These may include atmosphere monitoring and warning
devices.
Be sure to keep the operating instructions in a place that is accessible to users of this
unit.

6
PRECAUTIONS FOR SAFE OPERATION
Do not use the unit outdoors. Exposure to rain may cause leakage and/or electric shock.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit in a location capable of bearing the total combined weight (product + optional
accessories + stored items). After installing the unit, be absolutely sure to take precautions to
prevent the unit from falling over. If the unit is installed in a location which is not strong enough or if
the proper precautions are not taken, the unit may fall over and cause injuries.
Do not install the unit where there are high levels of moisture or where it may be splashed with
water. Installing the unit where there are high levels of moisture or where it may be splashed with
water may cause the insulation to deteriorate and give rise to leakage and/or electric shock.
Do not install the unit in a location where flammable or volatile substances are present.
Installing the unit in a location where flammable or volatile substances are present may cause
explosions and/or a fire.
Do not install the unit in a location where corrosive gases such as acids are present. Installing
the unit in a location where corrosive substances are present may cause electric components to
corrode, leading to leakage and/or electric shock due to the deterioration of insulation resulting from
corroded electrical components.
Do not place this unit in a location where it is difficult to disconnect the power supply plug.
Failure to disconnect the power supply plug may cause fire in the event of a problem or malfunction.
Be absolutely sure to earth (ground) the unit in order to prevent electric shock. Failure to earth
the product may give rise to electric shock. If necessary, ask a qualified contractor to do this work.
Do not connect the earth wire to a gas pipe, water pipe, or lightning rod when earthing the
unit. Earthing the unit improperly may give rise to electric shock.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use of
any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit except in a sealed container. Such
substances may cause explosion or fire if they leak.
Never insert metal objects such as pins and wires into any vent, gap or outlet on the unit. This
may cause electric shock or injury by accidental contact with moving parts.
When handling harmful samples (for example, those which consist of toxic, pathogenic, or
radioactive substances), install the unit inside a designated isolation facility. If the unit is
installed in a location which is not an isolation facility, there may be detrimental effects on both people
and the natural environment.
WARNING

7
PRECAUTIONS FOR SAFE OPERATION
Before proceeding with maintenance or checking of the unit, set the power switch to OFF and
disconnect the power supply plug. Performing the work while power is still flowing to the product or
while the power-supply plug is still connected may give rise to electric shock and/or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
Wear protective gloves and mask during maintenance. Touching or inhaling chemicals or
aerosols from around the unit may be detrimental to health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on top of the unit as this may cause electric shock or short circuit if
the liquid is spilled.
Never damage the power supply cord or power supply plug (by breaking, adapting, placing near
a source of heat, bending with force, twisting, pulling, adding weight, or binding). A damaged
power supply cord or power supply plug may cause electric shock, short circuit, or fire.
Do not use the supplied power cord for other electrical equipment. Such power supply cord may
cause fire or electric shock.
Never disassemble, repair, or modify the unit yourself. A high-voltage area is located inside the
unit. Any work carried out by an unauthorized personnel may result in electric shock. Contact our
sales representative or agent for maintenance or repair.
Make sure the power supply plug is pushed fully in. Faulty insertion of the power supply plug may
cause electric shock or fire due to generation of heat. Never use a damaged power supply plug or
loose power outlet.
Disconnect the power supply plug if there is anything wrong with the unit. Continued abnormal
operation may cause electric shock or fire.
Grip the power supply plug when disconnecting the power supply cord from the outlet. Pulling
the power supply cord may cause electric shock or short circuit.
Remove dust from the power supply plug periodically. Dust on the power supply plug may cause
insulation failure due to moisture and thus cause a fire. Disconnect the power supply plug and wipe it
with a dry cloth.
Disconnect the power supply plug before moving the unit. Take care not to damage the power
supply cord. A damaged cord may cause electric shock or fire.
Disconnect the power supply cord when the unit is not in use for long periods. Keeping the unit
connected may cause electric shock, leakage, or fire due to the deterioration of insulation.
WARNING

8
PRECAUTIONS FOR SAFE OPERATION
If the unit is to be stored unused in an unsupervised area for a long period, ensure that children do
not have access and that doors cannot be closed completely.
Ask a qualified contractor to carry out disassembly and disposal of the unit. Leaving the unit in
a location that can be accessed by third parties may result in unexpected accidents (e.g. the unit may
be used for unintended purposes).
Do not leave the plastic bags used for packing in a place where they can be reached by small
children as this may result in unexpected accidents such as suffocation.
Always use the removal power supply cord that is provided. Other power supply cord may cause
electric shock or fire.
When using CO2 gas for control, make sure that there is adequate ventilation. Using CO2 gas in a
small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition,
when opening the incubator doors, do not directly inhale the air in the chamber.
When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the
connections are secure and that no gas will leak. Be sure to use the specified pressure. Using
an incorrect gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation
due to gas leak.
Install the incubator in a location with adequate ventilation. If adequate ventilation cannot be
provided, then install an alarm system using CO2 and O2 densitometers.
Do not look directly at UV light. UV light is harmful to the eyes.
The CO2 incubator must be dismantled and disposed of by qualified personnel only. If the CO2
incubator is left where outsiders enter, it may result unexpected accident (for example, children to
become locked inside).
WARNING

9
PRECAUTIONS FOR SAFE OPERATION
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may
cause fire resulting from abnormal heating.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be
sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for
the safety of service personnel.
Do not climb on top of the incubator or place any object on it. By doing so you may damage the
incubator or knock it over, which may result in injury.
Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may
result in cuts or abrasions from sharp edges or corners.
Do not damage the glass or give it a shock. The Inner door in the CO2 Incubator are tempered glass,
but they can be broken or cause injury if they are used incorrectly.
To ensure the safety of the service engineer, submit a safety check sheet with the required
items filled out. This is provided as the photocopiable “Safety Check Sheet” at the end of these
operating instructions
CAUTION

10
LABELS ON INCUBATOR
Users are advised to read carefully the warnings and cautions contained on stickers at key locations on the
interior and exterior of the incubator.
Possible
Danger
Warning/Caution Type
Location of Danger
Warning/Caution Label Description of Danger
Burns Hot Surface
Cooling Unit &
Heat Cover
Avoid touching the cooling unit
and heat cover, which can reach
high temperatures and may cause
burns.
Personal
injury
Hazardous UV Light
Interior
The UV light is hazardous. Never
turn on the UV lamp without the
cover.
Personal
injury
Gas Poisoning or
Oxygen Deprivation
Environment
When using CO
2 gas, ensure
there is adequate ventilation.
Using CO2 gas in a small room
without adequate ventilation may
cause gas poisoning or oxygen
deprivation. In addition, when
opening the incubator doors, do
not directly inhale the air in the
chamber.
Personal
injury
Gas Poisoning or
Oxygen Deprivation
Interior
Excessive pressure may cause
gas supply lines inside the
incubator to come loose, which
may result in gas poisoning or
oxygen deprivation due to the
escape of gas.
Personal
injury
Electric shock
Top cover
Never remove this cover. Only a
service personnel remove the
cover to avoid the electric shock.

11
SYMBOLS ON INCUBATOR
The following symbols are attached to the incubator:
Attached to covers that access high-voltage electrical components to prevent electric
shock. Only a qualified engineer or service personnel should be allowed to open
these covers.
Indicates an ultraviolet light (UV) caution.
Indicates that caution is required. Refer to precautions for safe operation for details.
Indicates a hot surface.
Indicates an earthing.
Indicates “ON” for a power switch.
Indicates “OFF” for a power switch.
ENVIRONMENTAL CONDITIONS
This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):
■ Indoor use;
■ Altitude up to 2000 m;
■ Temperature 5 oC to 40 oC;
■ Maximum relative humidity 80 % for temperature up to 31 oC decreasing linearly to 50 % relative
humidity at 40 oC;
■ Mains supply voltage fluctuations up to ±10 % of the nominal voltage;
■ Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;
■ Temporary OVERVOLTAGES occurring on the mains supply;
■ Applicable pollution degree of the intended environment (POLLUTION DEGREE 2 in most cases);

12
INCUBATOR COMPONENTS
Unit
1 2 3 Tray 4 5
6
7*1
8
*1: When an optional UV system set MCO-170UVS is installed.
*2: Type FG-7P or FG-7PL.
(When some inner
attachments are removed)
Leveling ｆeet
Handle
Control
Panel
12
19
20
9
(When 9 is removed)
Sample air outlet cap
17 16 15*2 14 13
Lower right side
Removable power
supply cord port
18
10
11
Access port
cover Silicon cap
Rear side

13
INCUBATOR COMPONENTS
1. Outer door: The outer door is held to the frame with the magnetic seal. The door heater is installed in
the door panel. The door opening is reversible. Contact our sales representative or agent to change the
door hinge from left to right or vice versa.
2. Inner door: The inner door is made of tempered glass. Do not subject the glass to excessive impact.
Contact our sales representative or agent to change the inner door to optional small doors (MCO-170ID).
3. Tray catches: Insert tray to fit the concave portion on the chamber.
4. Fan cover (above the duct): Serves as the inlet for circulating air. It is removable.
5. Duct: The duct for the path for circulating air. It is removable.
6. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave.
7. UV lamp*1: This UV lamp does not generate ozone. For replacement, contact our sales representative
or agent.
8. Humidity control bar: Automatically reduces condensation caused by the external environment when
the door is frequently opened and closed. The humidity control bar has a bactericidal effect due to its
plated surface. However, it is recommended that it is replaced every 5 to 6 years to maintain this effect
(The duration of bactericidal effect differs depending on the environment).
9. Switch cover: Prevents shutting down of the unit in case of accidental pressing of the main power
switch.
10. Access port: Place the silicon caps on both the outside and inside of the port when the port is not
being used.
11. Remote alarm terminal: Sends the alarm to a remote location by connecting to an external alarm unit.
Refer to page 15.
12. USB port: Insert USB memory device to export operations and alarms log. Refer to pages
38,39~40,44~45.
13. Power supply cord cover plate: Prevents the power supply cord becoming detached.
14. Connecting port A for CO2 gas pipe: Refer to page 19 for gas cylinder connection.
Note: When the optional MCO-21GC gas auto-changer is installed, both ports A and B are available. Refer
to page 60.
15. Glow starter*2: Starts the glow for the UV lamp.
16. Power switch: This is the main switch for the incubator (ON-“I”, OFF-“O”). It also functions as an
overcurrent breaker.
17. Sample air outlet: This also functions as an internal gas outlet. In normal use, cover this outlet with
the sample air outlet cap.
18. Door switch (Beneath the label): Detects the door opening/closing and stops the fan and
electromagnetic valve for CO2 when the door is open. The UV lamp*1 is also deactivated by the door
opening.
19. Humidifying pan: Fill with sterile distilled water.
20. Humidifying pan cover: Prevents UV light entering the chamber.
*1: When an optional UV system set MCO-170UVS is installed.
*2: Type FG-7P or FG-7PL.

14
INCUBATOR COMPONENTS
Control panel
① OLED (Organic Light Emitting Diode) display
The present chamber temperature/CO2 density, menu screen or input screen, etc. are displayed.
② POWER/ALARM indicator
In normal operation, the green LED lamp lights up, and during alarm condition, the red LED lamp blinks to
indicate alarm.
③ Up/down/left/right key (△▽◁▷)
On the setting screen, pressing this key changes the display.
If the left / right key (◁▷) is displayed at the message of the home screen, you can switch the contents of
the message number with the left and right keys.
④ Menu key (MENU/HOME)
On the “home screen”: pressing this key leads the menu screen. It is possible to set various setting on the
menu screen.
On the screen “other than the home screen”: pressing this key leads the home screen.
⑤ Cancel key (CANCEL）
On the setting screen, pressing this key changes into the screen of one level up.
⑥ Buzzer stop key (BUZZER STOP)
The buzzer is silenced by pressing this key when the buzzer sounds.
However, when the ring back is ON, the buzzer will sound again when the set time of ring back passed and
the alarm state still continues. Refer to page 53 and 67~69.
Note: It is not possible to silence the buzzer for the high limit temperature alarm.
⑦ Enter key (ENTER)
Press this to select the menu item. Press this key to enter the required value during the setting procedure.
⑧ USB port
Insert a USB memory device to export data logs.
 Do not insert anything other than a USB memory device.

①
②
③
④
⑤
⑥
⑦
⑧

15
INCUBATOR COMPONENTS
Remote alarm terminal
The alarm of this product can be transferred to a remote location by connecting the external alarm unit to
the remote alarm terminal. For the behaviour of remote alarm output, refer to pages 67~69.
The terminal of the remote alarm is installed at the
rear upper right of the unit (see the figure opposite).
The alarm outputs from this terminal. Contact
capacity is DC 30 V, 2 A.
Table 1 shows the behaviour of the remote alarm
when the BUZZER STOP key is pressed.
Notes:
• The door alarm does not work remotely. Refer to
pages 68~69.
• Wiring of the remote alarm should be performed by
qualified service personnel.
Table 1. Behaviour of remote alarm when pressing BUZZER STOP key
Remote Alarm setting
(Refer to pages 48~54)
Connecting
terminal
Normal
condition
Abnormal condition
(Including in the cases of power outage and
of where the power supply plug is pulled out)
When pressing the BUZZER
STOP key
ON: Remote alarm setting not
connected with BUZZER
STOP key
COM.-N.C. Close Open Open (Maintain in abnormality)*
COM.-N.O. Open Close Close (Maintain in abnormality)*
OFF: Remote alarm setting
connected with BUZZER
STOP key
COM.-N.C. Close Open Close (Return to normal)
COM.-N.O. Open Close Open (Return to normal)
* In the case of Error01 (CO2 gas empty), Error11 (CO2 sensor error), the condition returns to normal.
Use a twisted shield wire for the connection.
Type: UL 2343, UL 2448, UL 2464, UL 2552, UL2623.
Length: 30 m max.
Remote alarm
terminal

16
INSTALLATION
Installation site
For correct operation of the incubator, install it in a location with the following conditions.
 Normal air environment
Install the incubator in an environment with normal air.
 Do not expose to direct sunlight
Do not install the incubator in a location where it will be exposed to direct sunlight. If the incubator is
operated in direct sunlight, performance will be adversely affected.
 Separate from heat sources
Do not install the incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.
Heat will adversely affect the performance of the incubator.
 Ambient temperature at least 5 C lower than set temperature
The control temperature of the incubator is at least 5 C higher than the ambient temperature. For example,
if the chamber is controlled at 37 C, the ambient temperature must be 32 C or less. Do not allow the
ambient temperature to become too high.
 Strong and level floor
Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the incubator
will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure that
the installation is stable. An unstable surface may result in vibration or noise.
 Separate from vibration products
Do not install the incubator near vibration products. Vibration may cause culture failure.
 Low humidity
Select a site with a relative humidity of 80 %R.H. or lower. Using the incubator in high humidity may result
in current leakage or electric shock.
 No inflammable or corrosive gas
Never install the incubator in a location where it will be exposed to inflammable or corrosive gas. Doing so
may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective casing,
resulting in current leakage or electric shock.
 No falling objects
Do not install the incubator in a location where there is the possibility of objects falling from above. Doing
so may result in damage or accident.

17
INSTALLATION
Installation
1. Remove the packing tape and clean up.
Remove all the tapes that are securing the doors and the inner attachments. Open the doors for ventilation.
If the outer panels are dirty, use a cloth to wipe them with a diluted neutral detergent (Undiluted detergent
can damage the plastic components. For the dilution, refer to the instructions on the detergent).
Wipe off the residual detergent with a wet cloth and then wipe off any moisture.
Note: Remove the cable tie around the power supply cord to prevent corrosion of the cord coating.
2. Set up the humidifying pan and humidifying pan cover (Fig. 1).
3. Install 3 trays (Fig. 2).
(When optional small doors (MCO-170ID) are installed)
To use the gastight split doors effectively, set 3 trays at the same level as shown in Fig 3 below.
At the same level
as bottom side of
each gastight
split door
Gastight split door Inner door
Fig. 3
Humidifying pan
Fig. 1
Humidifying pan cover
3 Trays
Fig. 2

18
INSTALLATION
4. Adjust the leveling feet.
Adjust the leveling feet by turning them
anticlockwise until the incubator is level (Fig. 4).
Note: Incubating on a sloping tray may adversely
affect the cultivation.
5. Earth the incubator.
Earth the incubator during installation to prevent electric shock. If there is no earth wire at the location,
consult with qualified service personnel.
 When an earth must be installed
If an earthed 3-pole outlet is not available, then an earth must be installed. Consult with qualified service
personnel.
 Installing an earth fault circuit breaker
If you must use the incubator in a moist or humid location, then it is recommended that an earth fault circuit
breaker be installed in the power supply circuit (i.e., the power supply at the incubator). Have the circuit
breaker installed by qualified service personnel.
 Double-stacking
For stacking the incubators securely, refer to the
procedure included with the optional
double-stacking bracket MCO-170PS or the
stacking plate MCO-170SB.
Note: Two hooks are attached at the upper rear of
the incubator. When stacking incubators, fix the
upper incubator to the wall with these hooks and
wire or chain (Fig. 5).
Note: When stacking the incubators with our CO2
incubator or another O2/CO2 incubator, use the
stacking plate MCO-170SB. Refer to Table 7 on
page 77.
 When the incubator is not in use
Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the
chamber is completely dry before closing the doors. Failure to do so may result in damage.
 Before moving the incubator
Before moving the incubator, empty the water from the humidifying pan, disconnect the power supply plug
from the outlet, and make sure that the power supply cord will not be damaged. Failure to do so may result
in electric shock or fire.
Fig. 4
Leveling feet
Shrink
Stretch
Fig. 5
Hooks
Wire

19
INSTALLATION
Connecting a CO2 gas cylinder
WARNING
When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the connections
are secure and that no gas will leak. Be sure to use the specified pressure. Using an incorrect gas or
pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to gas leak.
Install the incubator in a location with adequate ventilation. If adequate ventilation cannot be provided,
then install an alarm system using CO2 and O2 densitometers.
1. Get a CO2 gas cylinder ready and install an optional gas regulator MCO-010R.
Notes:
• Use a liquefied-CO2 gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.
• When MCO-010R is not available, install a gas regulator rated at 25 MPa(G) (250 kgf/cm2(G),
3600 psi(G)) for the primary side, and 0.25 MPa(G) (2.5 kgf/cm2(G), 36 psi(G)) for the secondary side.
2. Using the gas tube provided, connect the
connecting port A for the CO2 gas pipe and the gas
regulator of the CO2 gas cylinder.
Notes:
• By using two tube bands provided, connect the gas tube tightly to prevent it from coming off.
• Make sure that the gas tube is not folded.
• If the CO2 gas is supplied to multiple CO2 incubators from a single gas cylinder, a CO2 solid will be formed
in the gas regulator. The gas regulator safety valve will operate, and there may be an explosive sound.
3. After connecting the gas tube, make sure that no gas is leaking (ex. by using a gas leak detector spray).
4. Set the CO2 gas on the secondary side to 0.03 MPa(G)–0.1 MPa(G) (0.3 kgf/cm2(G)–1 kgf/cm2(G),
4.4 psi(G)–14.5 psi(G)) for gas injection. Recommended pressure: 0.03 MPa(G) (0.3 kgf/cm2(G), 4.4
psi(G)).
Note: As the pressure increases, the CO2 gas density variation will increase. Excessive pressure may
cause gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen
deprivation due to leaking gas. If gas lines come loose, the incubator must be repaired.
5. When there is no CO2 gas left and the CO2 gas empty alarm is activated, replace the empty gas cylinder.
Note: When the optional gas auto-changer MCO-21GC is installed, it switches the empty CO2 gas supply
line to the other automatically. Refer to pages 60~61.
Note:
· The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities are
found during inspection, replace the lines immediately.
· Close the valve of the CO2 gas cylinder when the CO2 gas is not in use.
Connecting port A for CO2 gas pipe
Lower right side

20
BEFORE COMMENCING OPERATION
Initial cleaning method
Before using the incubator for the first time, clean dirt (tape residue, smear, etc.) from the
chamber and the inner attachments thoroughly.
Keeping the chamber clean is essential for efficient performance. Clean the chamber and
all the inner attachments at least once a month. Use the following steps to clean the
incubator properly. When these steps are not sufficient to remove dirt, contact our sales representative
or agent.
1. Remove the inner attachments, referring to “Removing inner attachments” on pages 21~22.
2. Clean the inner attachments, the chamber inside walls, and the inner door gaskets with a cloth or
sponge soaked in neutral detergent, diluted by 5 % or less (Fig. 1). (Undiluted detergent can damage the
plastic components. For the dilution, refer to the instructions on the detergent.)
CAUTION
Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause
discoloration, corrosion, or rusting.
Be careful to keep the diluted detergent or water out of the temperature sensor, the CO2 gas injection hole,
the fan motor shaft bearing, and the inner sample air outlet (marked on Fig. 2 by ). Also, do not wash
the temperature sensor, CO2 sensor and the UV lamp using detergent. Doing so may cause failure.
3. Soak a piece of gauze or unwoven cloth in distilled water, wring it out and wipe off the residual detergent
thoroughly.
4. Wash the two silicon caps for the access port and the fan using the detergent and rinse them with
distilled water, and then autoclave them for sterilization (121 oC, 20 minutes).
5. Wipe the inside walls and the inner attachments thoroughly with an unwoven cloth soaked in alcohol for
disinfection. Be careful not to leave any alcohol residue.
6. Reinstall the inner attachments correctly and securely, referring to “Installing inner attachments” on page
23.
Fig. 1 Fig. 2
Inner door gasket Silicon cap
Temp. sensor
(UV lamp)
Humidity
control bar
図
CO2 sensor