Phcbi MCO-50ML User manual

MCO-50ML
Please read the operating instructions carefully before using this product, and keep the operating
instructions for future use.
See page 101 for model number.
Operating Instructions
O2/CO2Incubator

2
CONTENTS
INTRODUCTION ..........................................................................................4
PRECAUTIONS FOR SAFE OPERATION...................................................5
LABELS ON INCUBATOR..........................................................................10
SYMBOLS ON INCUBATOR ......................................................................11
ENVIRONMENTAL CONDITIONS ............................................................. 11
INCUBATOR COMPONENTS ....................................................................12
Unit ........................................................................................................12
Control panel .........................................................................................14
Remote alarm terminal ..........................................................................15
INSTALLATION...........................................................................................16
Installation site.......................................................................................16
Installation..............................................................................................17
Connecting a CO2gas cylinder .............................................................19
Connecting N2/O2gas cylinders ............................................................21
BEFORE STARTING OPERATION ............................................................22
Initial cleaning method...........................................................................22
Removing inner attachments.................................................................23
Installing inner attachments...................................................................25
Filling the humidifying pan.....................................................................26
FOR OPTIMAL CULTIVATION ...................................................................27
Precautions for cultures.........................................................................27
Preventing contamination......................................................................28
CORRECT OPERATION ............................................................................29
HOME SCREEN.........................................................................................30
MENU SCREEN .........................................................................................32
BASIC PARAMETERS ...............................................................................34
Setting temperature ...............................................................................34
Setting CO2gas.....................................................................................35
Setting CO2density ...............................................................................36
Setting O2gas........................................................................................37
Setting O2density..................................................................................38
Setting high limit temperature alarm......................................................39
Automatic N2(O2) gas cylinder switching..............................................40
Manual N2(O2) gas supply line A/B switching.......................................41
OPERATION/ALARM LOG.........................................................................42
Displaying operation log ........................................................................42
Exporting operation log..........................................................................45
Setting log interval .................................................................................47
Setting Unique ID ..................................................................................48
Displaying alarm log ..............................................................................49
Exporting alarm log................................................................................50
KEY LOCK FUNCTION ..............................................................................52
Setting key lock .....................................................................................52
Cancelling key lock................................................................................53

3
CONTENTS
ELECTRIC LOCK (OPTION)......................................................................54
Setting door lock....................................................................................54
Setting door lock delay time ..................................................................55
Specifying the method to unlock the outer door ....................................56
Unlocking the outer door with a User ID................................................57
Unlocking the outer door during a power outage or malfunction...........57
Registering user ID/password ...............................................................58
Deleting User IDs ..................................................................................60
ALARM PARAMETERS..............................................................................61
Setting temperature alarm.....................................................................61
Setting temperature alarm delay ...........................................................62
Setting CO2density alarm .....................................................................63
Setting CO2density alarm delay............................................................64
Setting O2density alarm........................................................................65
Setting O2density alarm delay ..............................................................66
Setting door alarm delay........................................................................67
Setting ring back delay ..........................................................................68
Setting remote alarm .............................................................................69
UV LAMP (OPTION)...................................................................................70
Using UV lamp.......................................................................................70
Setting UV lamp ON period ...................................................................71
UV lamp life indication...........................................................................72
Automatic extension of UV lamp ON period..........................................73
Lighting UV lamp for 24 hours...............................................................74
GAS AUTO CHANGER (OPTION) .............................................................75
Connecting CO2gas cylinders...............................................................75
Automatic CO2gas supply line switching ..............................................75
Manual CO2gas supply line A/B switching............................................77
OTHER PARAMETERS..............................................................................78
Setting date display format....................................................................78
Setting date ...........................................................................................79
Setting time............................................................................................80
Setting keypad clicking sound ...............................................................81
H2O2DECONTAMINATION (OPTION).......................................................82
H2O2decontamination ...........................................................................82
ALARMS, SAFETY, AND SELF-DIAGNOSIS.............................................86
ROUTINE MAINTENANCE ........................................................................90
TROUBLESHOOTING................................................................................91
DISPOSAL OF UNIT...................................................................................94
SPECIFICATIONS ......................................................................................99
PERFORMANCE......................................................................................101
SAFETY CHECK SHEET .........................................................................102

4
INTRODUCTION
■Read the operating instructions carefully before using the product and follow the instructions for safe
operation.
■PHC Corporation takes no responsibility for safety if the product is not used as intended or is used with
any procedures other than those given in the operating instructions.
■Keep the operating instructions in a suitable place so that they can be referred to as necessary.
■The operating instructions are subject to change without notice for improvement of performance or
function.
■Contact our sales representative or agent if any page of the operating instructions is lost or the page
order is incorrect, or if the instructions are unclear or inaccurate.
■No part of the operating instructions may be reproduced in any form without the express written
permission of PHC Corporation.
IMPORTANT NOTICE
PHC Corporation guarantees this product under certain warranty conditions. However, please note that
PHC Corporation shall not be responsible for any loss or damage to the contents of the product.
<Intended Use>
This equipment is designed for cell and tissue culture for laboratory use.

5
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with the operating instructions
as they contain important safety advice.
Items and procedures are described so that this unit can be used correctly and safely.
Following these precautions will prevent possible injury to the user and any other person.
Precautions are illustrated in the following way:
WARNING
Warning indicates a potentially hazardous situation which, if not avoided,
could result in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbols have the following meaning;
This symbol means caution.
T
his symbol means an action is prohibited.
T
his symbol means an instruction must be followed.
WARNING
As with any equipment that uses CO2gas, there is a likelihood of oxygen depletion in the vicinity
of the equipment. It is important that you assess the work site to ensure there is suitable and
sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a
safe environment must be considered. These may include atmosphere monitoring and warning
devices.

6
PRECAUTIONS FOR SAFE OPERATION
Do not use the unit outdoors. Exposure to rain may cause leakage and/or electric shock.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit in a location capable of bearing the total combined weight (product + optional
accessories + stored items). After installing the unit, be absolutely sure to take precautions to
prevent the unit from falling over. If the unit is installed in a location which is not strong enough or if
the proper precautions are not taken, the unit may fall over and cause injuries.
Do not install the unit where there are high levels of moisture or where it may be splashed with
water. Installing the unit where there are high levels of moisture or where it may be splashed with
water may cause the insulation to deteriorate and give rise to leakage and/or electric shock.
Do not install the unit in a location where flammable or volatile substances are present.
Installing the unit in a location where flammable or volatile substances are present may cause
explosions and/or a fire.
Do not install the unit in a location where corrosive gases such as acids are present. Installing
the unit in a location where corrosive substances are present may cause electric components to
corrode, leading to leakage and/or electric shock due to the deterioration of insulation resulting from
corroded electrical components.
Do not place this unit in a location where it is difficult to disconnect the power supply plug.
Failure to disconnect the power supply plug may cause fire in the event of a problem or malfunction.
Be absolutely sure to earth (ground) the unit in order to prevent electric shock. Failure to earth
the product may give rise to electric shock. If necessary, ask a qualified contractor to do this work.
Do not connect the earth wire to a gas pipe, water pipe, or lightning rod when earthing the
unit. Earthing the unit improperly may give rise to electric shock.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use of
any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit except in a sealed container. Such
substances may cause explosion or fire if they leak.
Never insert metal objects such as pins and wires into any vent, gap or outlet on the unit. This
may cause electric shock or injury by accidental contact with moving parts.
When handling harmful samples (for example, those which consist of toxic, pathogenic, or
radioactive substances), install the unit inside a designated isolation facility. If the unit is
installed in a location which is not an isolation facility, there may be detrimental effects on both people
and the natural environment.
WARNING

7
PRECAUTIONS FOR SAFE OPERATION
Before proceeding with maintenance or checking of the unit, set the power switch to OFF and
disconnect the power supply plug. Performing the work while power is still flowing to the product or
while the power-supply plug is still connected may give rise to electric shock and/or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
Wear protective gloves and mask during maintenance. Touching or inhaling chemicals or
aerosols from around the unit may be detrimental to health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on top of the unit as this may cause electric shock or short circuit if
the liquid is spilled.
Never damage the power supply cord or power supply plug (by breaking, adapting, placing near
a source of heat, bending with force, twisting, pulling, adding weight, or binding). A damaged
power supply cord or power supply plug may cause electric shock, short circuit, or fire.
Do not use the supplied power cord for other electrical equipment. Such power supply cord may
cause fire or electric shock.
Never disassemble, repair, or modify the unit yourself. A high-voltage area is located inside the
unit. Any work carried out by an unauthorized personnel may result in electric shock. Contact our
sales representative or agent for maintenance or repair.
Make sure the power supply plug is pushed fully in. Faulty insertion of the power supply plug may
cause electric shock or fire due to generation of heat. Never use a damaged power supply plug or
loose power outlet.
Disconnect the power supply plug if there is anything wrong with the unit. Continued abnormal
operation may cause electric shock or fire.
Grip the power supply plug when disconnecting the power supply cord from the outlet. Pulling
the power supply cord may cause electric shock or short circuit.
Remove dust from the power supply plug periodically. Dust on the power supply plug may cause
insulation failure due to moisture and thus cause a fire. Disconnect the power supply plug and wipe it
with a dry cloth.
Disconnect the power supply plug before moving the unit. Take care not to damage the power
supply cord. A damaged cord may cause electric shock or fire.
Disconnect the power supply cord when the unit is not in use for long periods. Keeping the unit
connected may cause electric shock, leakage, or fire due to the deterioration of insulation.
WARNING

8
PRECAUTIONS FOR SAFE OPERATION
If the unit is to be stored unused in an unsupervised area for a long period, ensure that children do
not have access and that doors cannot be closed completely.
Ask a qualified contractor to carry out disassembly and disposal of the unit. Leaving the unit in
a location that can be accessed by third parties may result in unexpected accidents (e.g. the unit may
be used for unintended purposes).
Do not leave the plastic bags used for packing in a place where they can be reached by small
children as this may result in unexpected accidents such as suffocation.
Use the reagent specified by our company for H2O2decontamination. Using a different H2O2
solution may result in explosion or damage to the incubator.
When performing H2O2decontamination, securely close the internal and external doors. Failure to
do so may be harmful to health due to leakage of H2O2gas.
During H2O2decontamination, plug the access hole with the silicon cap that is provided. Failure to
do so may be harmful to health due to leakage of H2O2gas.
Always use the removal power supply cord that is provided. Other power supply cord may cause
electric shock or fire.
When using CO2/N2gas for control, make sure that there is adequate ventilation. Using CO2/N2gas
in a small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In
addition, when opening the incubator doors, do not directly inhale the air in the chamber.
When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the
connections are secure and that no gas will leak. Be sure to use the specified pressure. Using
an incorrect gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation
due to gas leak.
O2gas increases the susceptibility of substances to burn. Take care of the handling of flame in a
room where the incubator is installed.
Install the incubator in a location with adequate ventilation. If adequate ventilation cannot be
provided, then install an alarm system using CO2and O2 densitometers.
Do not look directly at UV light. UV light is harmful to the eyes.
Never start H2O2decontamination when “Warning: UV Bulb Life” is displayed in the message display
area. The UV resolve is not sufficient.
Do not use the unlock key to unlock the outer door during H2O2decontamination or during H2O2
gas resolve by UV. Doing so may cause harm to health from H2O2gas leakage.
WARNING

9
PRECAUTIONS FOR SAFE OPERATION
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may
cause fire resulting from abnormal heating.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be
sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for
the safety of service personnel.
Wear rubber gloves when handling the H2O2reagent. Direct contact with the H2O2reagent may
result in inflammation of the skin.
H2O2decontamination can be performed only for the chamber and chamber attachments with standard
specifications, and not for any other objects.
Perform H2O2decontamination with the chamber attachments arranged as specified by our
company. Arranging them in a different way may result in insufficient decontamination.
After H2O2decontamination has been completed, wear rubber gloves and use a non-woven cloth to
wipe off the residual H2O2fluid from the bottom of the chamber, any objects that were
decontaminated, and the bottoms of ducts.
Do not climb on top of the incubator or place any object on it. By doing so you may damage the
incubator or knock it over, which may result in injury.
Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may
result in cuts or abrasions from sharp edges or corners.
Do not damage the glass or give it a shock. The Inner door in the O2/CO2Incubator are tempered
glass, but they can be broken or cause injury if they are used incorrectly.
CAUTION

10
LABELS ON INCUBATOR
Users are advised to read carefully the warnings and cautions contained on stickers at key locations on the
interior and exterior of the incubator.
Possible
Danger
Warning/Caution Type
Location of Danger Warning/Caution Label Description of Danger
Burns Hot Surface
Cooling Unit &
Heat Cover
Avoid touching the cooling unit
and heat cover, which can reach
high temperatures and may cause
burns.
Personal
injury
Hazardous UV Light
Interior
The UV light is hazardous. Never
turn on the UV lamp without the
cover.
Personal
injury
Gas Poisoning or
Oxygen Deprivation
Environment
When using CO2gas, ensure
there is adequate ventilation.
Using CO2gas in a small room
without adequate ventilation may
cause gas poisoning or oxygen
deprivation. In addition, when
opening the incubator doors, do
not directly inhale the air in the
chamber.
Personal
injury
Gas Poisoning or
Oxygen Deprivation
Interior
Excessive pressure may cause
gas supply lines inside the
incubator to come loose, which
may result in gas poisoning or
oxygen deprivation due to the
escape of gas.
Fire Combustion-supporti
ng gas
Environment
O
2gas has a property that
enhances combustion. Take
care of the handling of flame in a
room where the incubator is
installed.
Personal
injury
Electric shock
Top cover
Never remove this cover. Only a
service personnel remove the
cover to avoid the electric shock.