Pickering Laboratories Pinnacle PCX User manual

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APPROVED
OperatOr Manual
Post-Column
Derivatization
Instrument
1280 Space Park Way
Mountain View
CA 94043
USA
800-654-3330 • 650-694-6700
650-968-0749 fax
www.pickeringlabs.com
[email protected]
http://pickeringlabs.blogspot.com
Cat. No. 0101-0008
Version 10, April 2016
Copyright © 2005 by Pickering Laboratories, Inc. All rights reserved. No Portion of this manual or
any artwork contained herein may be reproduced in any shape or form without the
express written consent of Pickering Laboratories, Inc.

I
Pinnacle Operators Manual
Pickering Laboratories Inc.
table Of COntents
1Getting Started
How to Use this Manual 1
Read this First! 2
Symbols and Warnings 2
Specifications 2
Site Requirements 4
1.1 Section 1 Introduction
What is Post-column Derivatization? 1.1
Requirements for a Successful Post-column Method 1.1
Design of a HPLC system 1.2
Designing a Post-column System 1.4
Design of the Pinnacle PCX 1.6
2.1 Section 2 Overview
Introduction 2.2
Check Valve 2.3
Column Connections and Column Oven 2.3
Reagent Pump 2.4
Reagent Valves 2.5
Reservoir Tray / Bottles 2.5
Fluidics Panel 2.7
Quick Change Reactor 2.8
Detector Connections 2.8
Pump Compartment 2.9
Gas Manifold 2.9
Display Module 2.10
Electronics Compartment 2.11
Back Panel 2.11
Safety Features in the Pinnacle PCX 2.13
Standard Configurations of Pinnacle PCX 2.14

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Pinnacle Operators Manual
Pickering Laboratories Inc.
3.1 Section 3 Installation
Site Requirements 3.2
Instrument Unpacking and Preparation 3.4
Unpacking 3.4
Gas Connections to Back Panel of Pinnacle PCX 3.5
Computer Connections 3.6
Software Installation 3.6
Pump and Autosampler Connections 3.8
Eluant Priming 3.10
Column and Guard Installation 3.10
Detector Connections 3.11
Reservoir Connections 3.12
Reagent Pump Preparation 3.13
Running a Chromatogram 3.14
Shutdown 3.15
4.1 Section 4 Pinnacle PCX Operation
Software Overview 4.1
Status Window 4.2
Menu Options/Functions 4.4
File
Control
Method
Sequence
Instrument
Configuring your Pinnacle PCX 4.6
Log Files 4.7
Creating and Editing Methods 4.7
Creating and Editing Sequences 4.10
Tutorials 4.11
Running post-column analysis using Pinnacle PCX 4.14
STOP/RESET Pinnacle PCX 4.17
Pinnacle Shutdown 4.17

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Pinnacle Operators Manual
Pickering Laboratories Inc.
5.1 Section 5 Maintenance
Maintenance Suggestions 5.1
Operating Suggestions 5.2
Proper Shutdown Procedures 5.3
Basic Maintenance Procedures 5.6
Reagent Filter(s) Replacement 5.7
Ambient Reactor Replacement 5.8
Replacement of Heated Reactor Cartridge 5.8
Valve Maintenance 5.9
Pump Seal Replacement 5.12
Fuse Replacement 5.16
Tubing Kit Guides 5.17
6.1 Section 6 Applications
Amino Acids 6.2
Introduction 6.2
Background 6.2-1
Basic Sample Preparation 6.2-2
Reagent Preparation 6.2-3
Post-Column Conditions 6.2-4
Procedure 6.2-5
Sample Chromatograms and Gradient Programs 6.2-6
Precautions 6.2-18
Carbamates 6.3
Introduction 6.3
Background 6.3
Basic Sample Preparation 6.3-2
Reagent Preparation 6.3-3
Post-Column Conditions 6.3-5
Analytical Procedure 6.3-5
Sample Chromatograms and Gradient Programs 6.3-5
Precautions 6.3-12

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Pinnacle Operators Manual
Pickering Laboratories Inc.
Glyphosate 6.4
Introduction 6.4
Background 6.4-1
Basic Sample Preparation 6.4-1
Reagent Preparation 6.4-2
Analytical and Post-Column Conditions 6.4-4
Analytical Procedure 6.4-4
Sample Chromatograms 6.4-5
Precautions 6.4-6
7.1 Section 7 Troubleshooting
Contact Pickering Laboratories For Support 7.1
Instrument Parameter Log 7.2
General Troubleshooting Advice 7.2
Common System Problems 7.3
Common Chromatography Problems 7.4
Common Column Problems 7.6
Application Specific Troubleshooting 7.7
• Amino Acids
• Carbamates
• Glyphosate
Amino Acid 7.7
Software Troubleshooting 7.8
Procedures 7.11
• To Remove Silica Deposits From Reactor
• To Remove Mineral Deposits In The Reactor From Hard Water
• To Remove Grease Deposits
• If Reagent Backflows Onto Column
• If TRIONE Backflows Onto Column
• If NaOH Is On Column
• To Remove Iron Contamination From Column
• To Pump RESTORE Through The Glyphosate Column

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Pinnacle Operators Manual
Pickering Laboratories Inc.
8.1 Appendices
Installation/Operational Qualitication of the Pinnacle PCX 8.2
Installation/Operational Qualitication of the Pinnacle PCX Data Sheet 8.7
Performance Qualifications of the Pinnacle PCX Data Sheet 8.8
Sample Instrument Parameter Log 8.15
Flow Diagram – Simplex 8.16
Flow Diagram – Duplex 8.17
Parts List for Pinnacle PCX 8.18
Recommended Consumables and Spare Parts 8.21
Limited Warranty 8.24
References 8.25
9.1 Index

VI
Pinnacle Operators Manual
Pickering Laboratories Inc.

1
GettinG Started
Pinnacle Operators Manual
Pickering Laboratories Inc.
GettinG started
How to Use this Manual
The Pinnacle PCX manual is designed to contain all of the information necessary for the installation, operation,
maintenance and troubleshooting of the Pinnacle PCX. It is designed to contain any and all information that you
may require during the lifetime of your Pinnacle PCX.
In addition to the general operation information, this manual also contains sections devoted to a particular
application. Since Pickering Laboratories provides the complete solution, we included the chromatograms,
operating conditions, and some troubleshooting information for our most commonly supported methods.
As well as instructional information, this manual also contains a section for recording service information such
as serial numbers, installation dates, service dates etc.
Each section of the manual is divided by a tab with the title of that section. For easy reference, simply select
the tab that you require based on the information you are looking for. For example, if you are running Amino
Acids, and have a question about the gradient program, simply go to the section titled “Amino Acids”.
1How to Use this Manual
2Read this First!
2Symbols and Warnings
2Specifications
4Site Requirements

2
GettinG Started
Pinnacle Operators Manual
Pickering Laboratories Inc.
Read this First!
Before attempting to install the Pinnacle PCX post-column derivatization instrument, it is vitally important that
you read this manual first, and attend to site, HPLC, and accessories requirements:
HPLC – Page 5
Gas Supply Requirements – Page 5
Reagent Reservoir bottles – Page 5
Computer – Page 5
Symbols and Warnings
Caution – this symbol indicates that caution must be used when dealing with this part.
Hot – this symbol is located on the Heated reactor, which can reach scalding temperatures.
Specifications
INSTRUMENT
Dimentions
21.50 H x 10.63 W x 18.25 D inches (54.0 x 26.7 x 46.4 cm), instrument only, doors closed
Weight
77lbs for Dual-pump systems
67lbs for Single-pump systems
Reagent Pumps
Max operating pressure 500 psi (35bar)
Flow rate range 50μl –1500μl/minute
Refill cycle 60 seconds
Heated Reactor
5°C above ambient to 130°C
Thermal Safety switch limits temperature to 150°C
Stability +/- 0.5°C
Accuracy +/- 1°C
!
!