Plexon Multi-Wavelength Photometry User manual

Multi-Wavelength Photometry
Quick Start Guide
Photometry
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(Initial Issue - September, 2020)
Plexon Multi-Wavelength Photometry Quick Start Guide Page 1 of 11

Photometry
Introduction
Plexon’s multi-wavelength photometry system has the capability to deliver multiple
wavelengths of excitation light and the ability to detect multiple wavelengths of emitted
light. The photometry system oers three excitation ranges centered at 560 nm, 465 nm, and
410 nm and two detection ranges centered at 600 nm and 525 nm. The system can monitor
multiple brain regions and track behavior simultaneously, which is useful in studying the
connection between dierent brain regions and the neural correlates of behavior.
Photometry System function
The uorescence of genetically encoded calcium indicators (GECIs), such as GCaMP
proteins, enables real-time optical monitoring and recording of neural activity of deep
brain structures in behaving animals. This camera-based system includes three built-in
LEDs for excitation and two optical sensors, which allow for detection of green and red
uorescence simultaneously. A branching patch cable provides the user with the ability to
record from multiple brain regions or multiple animals. The system interleaves the 410 nm
and 465 nm LEDS and the camera detecting green uorescence captures light for each of
these wavelengths independently, which allows the user to identify the calcium-dependent
uorescence (isobestic control).
This Quick Start Guide explains how to congure the Plexon® MultiWavelength Multi-ber
Photometry System (Photometry System).
Page 2 of 11Plexon Multi-Wavelength Photometry Quick Start Guide

Multi-Wavelength Photometry System and
Component Overview
The Photometry System components and connections are shown below.
The individual components are described in the pages that follow.
The Photometry System includes the following components:
Photometry
1. AC Power Cord
2. Power Supply
3. Multi-ber patch cable
4. Digtial IO Status Indicator
5. Trigger Box to Behavioral Camera Cable
6. USB cable type A to type B
7. Trigger Box
8. Trigger Box to Photometry Module cable
9. Multi-wavelength Multi-ber Photometry Module
10. Digital Input Generator
11. Trigger Box to Photometry Camera Cable (x2)
12. USB3 cable type A to type B with screwlocks (x2)
13. Fiber stub implants
14. USB cable type A to type mini-B
15. Photometry Behavioral Camera & Trigger Board
16. Computer
Page 3 of 11Plexon Multi-Wavelength Photometry Quick Start Guide
1.
2.
3.4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
See Appendix A for more detailed part information

Photometry Hardware Setup
Each researcher will need to evaluate the experimental set up to determine the
optimal location for the placement of the photometry hardware. The multi-
ber patch cable will be connected directly to the photometry module and will need
to be positioned so that the terminating end of this patch cable can be connected to the
subject.
Setup Instructions
1. Verify that the computer is turned o (powered down).
2. Connect the cameras on the Multi-wavelength Multi-ber Photometry Module (9) to the Computer (16)
using the two USB3 cable type A to type B with screwlocks (12).
3. Next, connect the Cameras located on the Multi-wavelength Multi-ber Photometry Module (9) to the
Trigger Box (7) using the two Trigger Box to Photometry Camera cables (11).
4. Now, connect the Multi-wavelength Multi-ber Photometry Box (9) to the Trigger Box via the Trigger Box
to Photometry cable (8).
Page 4 of 11Plexon Multi-Wavelength Photometry Quick Start Guide
Photometry Module Trigger Box
IMPORTANT NOTE: Make sure to plug the cable from camera 1 on the photometry box
into the rst Trigger Box connector. The cable from Camera 2 should be plugged into the
second Trigger Box connector.

Page 5 of 11Plexon Multi-Wavelength Photometry Quick Start Guide
5. Connect the Trigger Box (7) to a power source with the AC Power cord (1) and
Power Supply (2).
6. Connect the Trigger Box (7) to the Computer (16) using the USB cable type A to type B (6).
7. Connect the Trigger Box (7) to the Photometry Behavioral Camera (15) using the Trigger Box to
Behavioral Camera Cable (5).
8. Connect the Computer (16) to the Photometry Behavioral Camera (15) using the USB cable type A to
type mini-B (14).

Page 6 of 11Plexon Multi-Wavelength Photometry Quick Start Guide
9. Connect the provided keyboard and mouse to the computer.
10. Insert the license key into one of the built in USB ports.
11. Connect the multi-ber patch cable (3) to the Multi-ber Multi-wavelength Photometry Module
(9).
12. Turn on the Trigger Box.
13. You can now turn on the computer and begin.
Key Collar
Slot
Patch cable
Photometry Module
CAUTION
All system components need to be connected, plus the Trigger Box power switch
needs to be in the “ON” position, before the software starts.
If started rst, the software will not recognize the photometry system hardware and either
will not start or will switch to File mode.

Page 7 of 11Plexon Multi-Wavelength Photometry Quick Start Guide
Appendix A - Part List (Name, Part Number, SL)
1. AC Power Cord
2. Power Supply / Plexon 08-06-A-37 / 40612
3. Multi-ber patch cable / Plexon 08-60-A-04-C /
4. Digital IO Status Indicator (optional) / Plexon 07-04-A-04-C / 20118

Page 8 of 11Plexon Multi-Wavelength Photometry Quick Start Guide
5. Trigger Box to Behavioral Camera Cable / Plexon 06-06-A-12-A / 41004-015
6. USB cable type A to type B / 40783
7. Trigger Box / Plexon 07-12-A-00-P3 / 92304
8. Trigger Box to Photometry Box Cable / Plexon 06-06-A-14 / 92301-003

Page 9 of 11Plexon Multi-Wavelength Photometry Quick Start Guide
9. Multi-wavelength Multi-ber Photometry Module / Plexon 08-61-A-07-A
10. Digital Input Generator (optional) / Plexon 07-04-A-03-B / 27901
11. Trigger Box to Photometry Camera Cable / Plexon 06-06-A-13 / 41005-015
12. USB3 cable type A to type B with screwlocks (x2) / 41018-003, 41018-005

Page 10 of 11Plexon Multi-Wavelength Photometry Quick Start Guide
13. Fiber stub implants / Plexon 08-44-A-10 / 90880
14. USB cable type A to type mini-B / 40520
15. Behavioral Camera / Plexon 07-04-A-24-A /

Photometry
Page 11 of 11
Assistance Installing and Conguring the Multi-Wavelength
Photometry System
If you would like assistance conguring the Multi-Wavelength Photometry System and
settings for your experiment, please contact Plexon at [phone] or
suppor[email protected]om.
Plexon Multi-Wavelength Photometry Quick Start Guide