Agilent Technologies 1260 Infinity G1315C User manual

Agilent Technologies
Agilent 1260 Infinity
Diode Array and Multiple
Wavelength Detector
User Manual

Agilent 1260 Infinity DAD and MWD User Manual
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© Agilent Technologies, Inc. 2006-2017,
2018
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Manual Part Number
G1315-90015 Rev. C
Edition
07/2018
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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cated conditions are fully
understood and met.

Agilent 1260 Infinity DAD and MWD User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1260 Infinity Diode Array and Multiple
Wavelength Detector modules:
•G1315C - 1260 DAD VL+
•G1365C - 1260 MWD
•G1315D - 1260 DAD VL
•G1365D - 1260 MWD VL
1Introduction
This chapter gives an introduction to the detector, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your
system and the installation of your module.
4 Using the Detector
This chapter provides information on how to set up the detector for an
analysis and explains the basic settings.
5 How to optimize the Detector
This chapter provides information on how to optimize the detector.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.

4 Agilent 1260 Infinity DAD and MWD User Manual
In This Guide…
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
8 Test Functions
This chapter describes the detector’s built in test functions.
9 Maintenance
This chapter describes the maintenance of the detector.
10 Parts for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the detector to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity DAD and MWD User Manual 5
Contents
Contents
1 Introduction 9
Introduction to the Detector 10
Optical System 11
System Overview 14
Bio-inert Materials 16
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
3 Installing the Module 37
Unpacking the Detector 38
Optimizing the Stack Configuration 40
Installation Information on Leak and Waste Handling 44
Installing the Detector 48
Flow Connections to the Detector 51
Installing Capillaries 54
Setting up the LAN access 60
4 Using the Detector 61
Leak and Waste Handling 62
Setting up an Analysis 63
Special Settings of the Detector 78
Special Setups with Multiple DAD-MWDs 92
Solvent Information 93
5 How to optimize the Detector 99
Introduction 100
Optimization Overview 101
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 103
Optimizing Selectivity 113

6 Agilent 1260 Infinity DAD and MWD User Manual
Contents
6 Troubleshooting and Diagnostics 117
Overview of the Module’s Indicators and Test Functions 118
Status Indicators 119
User Interfaces 121
Agilent Lab Advisor Software 122
7 Error Information 123
What Are Error Messages 125
General Error Messages 126
Detector Error Messages 134
8 Test Functions 143
Self-test 144
Filter Test 145
Intensity Test 148
Holmium Oxide Test 151
ASTM Drift and Noise Test 154
Cell Test 156
Using the Built-in Test Chromatogram 158
Wavelength Verification and Calibration 160
9 Maintenance 163
Introduction to Maintenance 164
Cautions and Warnings 165
Overview of Maintenance 167
Cleaning the Module 168
Exchanging a Lamp 169
Exchanging a Flow Cell 172
Maintenance of Standard, Semi-Micro or Micro Flow Cell 176
Maintenance of High Pressure Flow Cell 180
Replacing Capillaries on a Standard Flow Cell 182
Replacing Capillaries on a Semi-Micro and Micro Flow Cell 188
Nano Flow Cell - Replacing or Cleaning 192
Cleaning or Exchanging the Holmium Oxide Filter 197
Correcting Leaks 200
Replacing Leak Handling System Parts 201
Replacing the CompactFlash Card (G1315C/G1365C only) 202
Replacing the Module’s Firmware 203

Agilent 1260 Infinity DAD and MWD User Manual 7
Contents
10 Parts for Maintenance 205
Overview of Maintenance Parts 206
Standard Flow Cell 208
Standard Flow Cell Bio-inert 210
Semi-Micro Flow Cell Parts 212
Micro Flow Cell 214
Prep Flow Cell - SST 216
Prep Flow Cell - Quartz 218
Nano Flow Cells 220
High Pressure Flow Cell 224
Accessory Kits 226
11 Identifying Cables 229
Cable Overview 230
Analog Cables 232
Remote Cables 234
BCD Cables 237
CAN/LAN Cables 239
Agilent 1200 module to PC 240
12 Hardware Information 241
Firmware Description 242
Electrical Connections 245
Interfaces 247
Setting the 8-bit Configuration Switch 254
Instrument Layout 258
Early Maintenance Feedback (EMF) 259
13 LAN Configuration 261
What you have to do first 262
TCP/IP parameter configuration 263
Configuration Switch 264
Initialization mode selection 265
Dynamic Host Configuration Protocol (DHCP) 269
Link configuration selection 272
Automatic Configuration with BootP 273
Storing the settings permanently with Bootp 283
Manual Configuration 284

8 Agilent 1260 Infinity DAD and MWD User Manual
Contents
14 Appendix 291
Safety Information 292
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 295
Radio Interference 296
Sound Emission 297
UV-Radiation 298
Solvent Information 299
Declaration of Conformity for HOX2 Filter 301
Installation of Stainless Steel Cladded PEEK Capillaries 302
Agilent Technologies on Internet 308

9
Agilent 1260 Infinity DAD and MWD User Manual
Agilent Technologies
1
Introduction
Introduction to the Detector 10
Optical System 11
System Overview 14
Leak and Waste Handling 14
Bio-inert Materials 16
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

10 Agilent 1260 Infinity DAD and MWD User Manual
1 Introduction
Introduction to the Detector
Introduction to the Detector
The detector is designed for highest optical performance, GLP compliance and
easy maintenance. It includes the following features:
•80 Hz data acquisition rate for (ultra-) fast LC applications (requires
internal hard disk, G1315C and G1365C only),
•data recovery (DRC) feature provides data-never-lost insurance (requires
internal hard disk, G1315C and G1365C only),
•RFID tags for all flow cells and UV-lamps provides traceable information
about these assemblies,
•long-life deuterium with RFID tag and tungsten lamps for highest intensity
and lowest detection limit over a wavelength range of 190 – 950 nm,
•no loss in sensitivity for up to eight wavelengths simultaneous,
•programmable slit from 1 – 16 nm for complete optimization of sensitivity,
linearity and spectral resolution,
•optional flow-cell cartridges with RFID tag (standard 10 mm13 μL,
semi-micro 6 mm5 μL, micro 3 mm2 μL, 80 nL, 500 nL, 10 mm, high
pressure 10 mm1.7 μL and prep-cells) are available and can be used
depending on the application needs,
•easy front access to lamps and flow cell for fast replacement, and
•built-in holmium oxide filter for fast wavelength accuracy verification,
•built-in temperature control for improved baseline stability,
•additional diagnostic signals for temperature and lamp voltage monitoring,
For specifications, see “Performance Specifications” on page 24.

Agilent 1260 Infinity DAD and MWD User Manual 11
Introduction
1
Optical System
Optical System
The optical system of the detector is shown in Figure below. Its illumination
source is a combination of a deuterium-arc-discharge lamp for the ultraviolet
(UV) wavelength range and a tungsten lamp for the visible (VIS) and
short-wave near-infrared (SWNIR) wavelength range. The image of the
filament of the tungsten lamp is focused on the discharge aperture of the
deuterium lamp by means of a special rear-access lamp design which allows
both light sources to be optically combined and share a common axis to the
source lens. The achromat (source lens) forms a single, focused beam of light
through the flow cell. Each cell room and lamp are separated by a quartz
window which can be cleaned or replaced. In the spectrograph, light is being
dispersed onto the diode array by a holographic grating. This allows
simultaneous access to all wavelength information.
Figure 1 Optical System of the Detector
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12 Agilent 1260 Infinity DAD and MWD User Manual
1 Introduction
Optical System
Lamps The light source for the UV-wavelength range is a deuterium lamp with a
shine-through aperture. As a result of plasma discharge in low-pressure
deuterium gas, the lamp emits light over the 190 nm to approximately 800 nm
wavelength range. The light source for the visible and SWNIR wavelength
range is a low noise tungsten lamp. This lamp emits light over the wavelength
range 470 – 950 nm.
Achromat
(Source Lens)
The achromat receives the light from both lamps and focuses it so that the
beam passes through the flow cell.
Holmium Oxide
Filter
The holmium oxide filter is electromechanically actuated. During the holmium
filter test it moves into the light path.
Cell Support
Window
The cell support window assembly separates the holmium filter area from the
flow cell area.
Flow Cell
Compartment
The optical unit has a flow cell compartment for easy access to flow cells. A
variety of optional flow cells can be inserted using the same quick, simple
mounting system. The flow cell can be removed to check the optical and
electronic performance of the detector without having influences from the
flow cell.
Spectrograph The spectrograph material is ceramic to reduce thermal effects to a minimum.
The spectrograph consists of the spectrograph lens, the variable entrance slit,
the grating and the photodiode array with front-end electronics. The
spectrograph lens refocuses the light beam after it has passed through the flow
cell. The sampling interval of the diode array is < 1 nm over the wavelength
range 190 – 950 nm. Depending on the wavelength this varies from 1.0 to 1.25
diodes per nanometer (for example a diode every 0.8 to 1 nm).
For a small wavelength range, the small non-linearity could be neglected. With
the wavelength range from 190 – 950 nm a new approach is required to
achieve wavelength accuracy over the full range. Each spectrograph is
calibrated individually. The calibration data is stored in the spectrograph on
an EEPROM. Based on these data, the built-in processors calculate absorbance
data with linear intervals (1.0, 2.0, …) between data points. This results in an
excellent wavelength accuracy and instrument-to-instrument reproducibility.
Variable Entrance
Slit System
The micro-slit system makes use of the mechanical properties of silicon
combined with the precise structuring capabilities of bulk micro-machining. It
combines the required optical functions — slit and shutter — in a simple and
compact component. The slit width is directly controlled by the
micro-processor of the instrument and can be set as method parameter.

Agilent 1260 Infinity DAD and MWD User Manual 13
Introduction
1
Optical System
Grating The combination of dispersion and spectral imaging is accomplished by using
a concave holographic grating. The grating separates the light beam into all its
component wavelengths and reflects the light onto the photodiode array.
Diode Array The diode array is a series of 1024 individual photodiodes and control circuits
located on a ceramic carrier. With a wavelength range from 190 – 950 nm the
sampling interval is < 1 nm.

14 Agilent 1260 Infinity DAD and MWD User Manual
1 Introduction
System Overview
System Overview
Leak and Waste Handling
The 1200 Infinity Series has been designed for safe leak and waste handling. It
is important that all security concepts are understood and instructions are
carefully followed.
Figure 2 Leak and waste handling concept (overview - typical stack configuration as
an example)
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Agilent 1260 Infinity DAD and MWD User Manual 15
Introduction
1
System Overview
The solvent cabinet (1) is designed to store a maximum volume of 6 L solvent.
The maximum volume for an individual bottle stored in the solvent cabinet
should not exceed 2.5 L. For details, see the usage guideline for the Agilent
1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has been
shipped with the solvent cabinet, electronic copies are available on the
Internet).
The leak pan (2) (individually designed in each module) guides solvents to the
front of the module. The concept covers also leakages on internal parts (e.g.
the detector’s flow cell). The leak sensor in the leak pan stops the running
system as soon as the leak detection level is reached.
The leak pan's outlet port (3, A) guides excessive overfill from one module to
the next, as the solvent flows into the next module’s leak funnel (3, B) and the
connected corrugated waste tube (3, C). The corrugated waste tube guides the
solvent to the next lower positioned module’s leak tray and sensor.
The waste tube of the sampler’s needle wash port (4) guides solvents to waste.
The condense drain outlet of the autosampler cooler (5) guides condensate to
waste.
The waste tube of the purge valve (6) guides solvents to waste.
The waste tube connected to the leak pan outlet on each of the bottom
instruments (7) guides the solvent to a suitable waste container.

16 Agilent 1260 Infinity DAD and MWD User Manual
1 Introduction
Bio-inert Materials
Bio-inert Materials
For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses
highest quality materials in the flow path (also referred to as wetted parts),
which are widely accepted by life scientists, as they are known for optimum
inertness to biological samples and ensure best compatibility with common
samples and solvents over a wide pH range. Explicitly, the complete flow path
is free of stainless steel and free of other alloys containing metals such as iron,
nickel, cobalt, chromium, molybdenum or copper, which can interfere with
biological samples. The flow downstream of the sample introduction contains
no metals whatsoever.
Tabl e 1 Bio-inert materials used in Agilent 1260 Infinity Systems
Module Materials
Agilent 1260 Infinity Bio-inert Quaternary Pump
(G5611A)
Titanium, gold, platinum-iridium, ceramic,
ruby, PTFE, PEEK
Agilent 1260 Infinity Bio-inert High-Performance Autosampler
(G5667A)
Upstream of sample introduction:
•Titanium, gold, PTFE, PEEK, ceramic
Downstream of sample introduction:
•PEEK, ceramic
Agilent 1260 Infinity Bio-inert Manual Injector
(G5628A)
PEEK, ceramic
Agilent 1260 Infinity Bio-inert Analytical Fraction Collector
(G5664A)
PEEK, ceramic, PTFE
Bio-inert Flow Cells:
Standard flow cell bio-inert, 10 mm, 13 µL, 120 bar ( 12 MPa) for MWD/DAD,
includes Capillary Kit Flow Cells BIO (p/n G5615-68755) (G5615-60022)
(for Agilent 1260 Infinity Diode Array Detectors DAD G1315C/D)
PEEK, ceramic, sapphire, PTFE
Max-Light Cartridge Cell Bio-inert ( 10 mm, V(s) 1.0 µL) (G5615-60018) and
Max-Light Cartridge Cell Bio-inert ( 60 mm, V(s) 4.0 µL) (G5615-60017)
(for Agilent 1200 Infinity Series Diode Array Detectors DAD G4212A/B)
PEEK, fused silica
Bio-inert flow cell, 8 µL, 20 bar (pH 1–12) includes Capillary Kit Flow Cells BIO
(p/n G5615-68755) (G5615-60005)
(for Agilent 1260 Infinity Fluorescence Detector FLD G1321B)
PEEK, fused silica, PTFE

Agilent 1260 Infinity DAD and MWD User Manual 17
Introduction
1
Bio-inert Materials
Bio-inert heat-exchanger G5616-60050
(for Agilent 1290 Infinity Thermostatted Column Compartment G1316C)
PEEK (steel-cladded)
Bio-inert Valve heads G4235A, G5631A, G5639A: PEEK, ceramic
(Al
2
O
3
based)
Bio-inert Connection capillaries Upstream of sample introduction:
•Titanium
Downstream of sample introduction:
•Agilent uses stainless-steel-cladded
PEEK capillaries, which keep the flow
path free of steel and provide
pressure stability to more than 600
bar.
Tabl e 1 Bio-inert materials used in Agilent 1260 Infinity Systems
Module Materials
NOTE
To ensure optimum bio-compatibility of your Agilent 1260 Infinity Bio-inert LC system, do
not include non-inert standard modules or parts to the flow path. Do not use any parts that
are not labeled as Agilent “Bio-inert”. For solvent compatibility of these materials, see
“Material Information” on page 93.

18 Agilent 1260 Infinity DAD and MWD User Manual
1 Introduction
Bio-inert Materials

19
Agilent 1260 Infinity DAD and MWD User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
Specifications 24
Specification Conditions 36
This chapter provides information on environmental requirements, physical
and performance specifications.

20 Agilent 1260 Infinity DAD and MWD User Manual
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line
voltage in the range described in Table 2 on page 23. Consequently there is no
voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.
WARNING
The module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical shock, when
the cover is opened and the module is connected to power.
➔Always unplug the power cable before opening the cover.
➔Do not connect the power cable to the instrument while the covers are removed.
CAUTION
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.