Agilent Technologies 1260 Infinity G1315C User manual

Agilent Technologies
Agilent 1260 Infinity
Diode Array and Multiple
Wavelength Detector
User Manual

Agilent 1260 Infinity DAD and MWD User Manual
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© Agilent Technologies, Inc. 2006-2017,
2018
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Manual Part Number
G1315-90015 Rev. C
Edition
07/2018
Printed in Germany
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Agilent 1260 Infinity DAD and MWD User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1260 Infinity Diode Array and Multiple
Wavelength Detector modules:
•G1315C - 1260 DAD VL+
•G1365C - 1260 MWD
•G1315D - 1260 DAD VL
•G1365D - 1260 MWD VL
1Introduction
This chapter gives an introduction to the detector, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your
system and the installation of your module.
4 Using the Detector
This chapter provides information on how to set up the detector for an
analysis and explains the basic settings.
5 How to optimize the Detector
This chapter provides information on how to optimize the detector.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.

4 Agilent 1260 Infinity DAD and MWD User Manual
In This Guide…
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
8 Test Functions
This chapter describes the detector’s built in test functions.
9 Maintenance
This chapter describes the maintenance of the detector.
10 Parts for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the detector to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity DAD and MWD User Manual 5
Contents
Contents
1 Introduction 9
Introduction to the Detector 10
Optical System 11
System Overview 14
Bio-inert Materials 16
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
3 Installing the Module 37
Unpacking the Detector 38
Optimizing the Stack Configuration 40
Installation Information on Leak and Waste Handling 44
Installing the Detector 48
Flow Connections to the Detector 51
Installing Capillaries 54
Setting up the LAN access 60
4 Using the Detector 61
Leak and Waste Handling 62
Setting up an Analysis 63
Special Settings of the Detector 78
Special Setups with Multiple DAD-MWDs 92
Solvent Information 93
5 How to optimize the Detector 99
Introduction 100
Optimization Overview 101
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 103
Optimizing Selectivity 113

6 Agilent 1260 Infinity DAD and MWD User Manual
Contents
6 Troubleshooting and Diagnostics 117
Overview of the Module’s Indicators and Test Functions 118
Status Indicators 119
User Interfaces 121
Agilent Lab Advisor Software 122
7 Error Information 123
What Are Error Messages 125
General Error Messages 126
Detector Error Messages 134
8 Test Functions 143
Self-test 144
Filter Test 145
Intensity Test 148
Holmium Oxide Test 151
ASTM Drift and Noise Test 154
Cell Test 156
Using the Built-in Test Chromatogram 158
Wavelength Verification and Calibration 160
9 Maintenance 163
Introduction to Maintenance 164
Cautions and Warnings 165
Overview of Maintenance 167
Cleaning the Module 168
Exchanging a Lamp 169
Exchanging a Flow Cell 172
Maintenance of Standard, Semi-Micro or Micro Flow Cell 176
Maintenance of High Pressure Flow Cell 180
Replacing Capillaries on a Standard Flow Cell 182
Replacing Capillaries on a Semi-Micro and Micro Flow Cell 188
Nano Flow Cell - Replacing or Cleaning 192
Cleaning or Exchanging the Holmium Oxide Filter 197
Correcting Leaks 200
Replacing Leak Handling System Parts 201
Replacing the CompactFlash Card (G1315C/G1365C only) 202
Replacing the Module’s Firmware 203

Agilent 1260 Infinity DAD and MWD User Manual 7
Contents
10 Parts for Maintenance 205
Overview of Maintenance Parts 206
Standard Flow Cell 208
Standard Flow Cell Bio-inert 210
Semi-Micro Flow Cell Parts 212
Micro Flow Cell 214
Prep Flow Cell - SST 216
Prep Flow Cell - Quartz 218
Nano Flow Cells 220
High Pressure Flow Cell 224
Accessory Kits 226
11 Identifying Cables 229
Cable Overview 230
Analog Cables 232
Remote Cables 234
BCD Cables 237
CAN/LAN Cables 239
Agilent 1200 module to PC 240
12 Hardware Information 241
Firmware Description 242
Electrical Connections 245
Interfaces 247
Setting the 8-bit Configuration Switch 254
Instrument Layout 258
Early Maintenance Feedback (EMF) 259
13 LAN Configuration 261
What you have to do first 262
TCP/IP parameter configuration 263
Configuration Switch 264
Initialization mode selection 265
Dynamic Host Configuration Protocol (DHCP) 269
Link configuration selection 272
Automatic Configuration with BootP 273
Storing the settings permanently with Bootp 283
Manual Configuration 284

8 Agilent 1260 Infinity DAD and MWD User Manual
Contents
14 Appendix 291
Safety Information 292
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 295
Radio Interference 296
Sound Emission 297
UV-Radiation 298
Solvent Information 299
Declaration of Conformity for HOX2 Filter 301
Installation of Stainless Steel Cladded PEEK Capillaries 302
Agilent Technologies on Internet 308

9
Agilent 1260 Infinity DAD and MWD User Manual
Agilent Technologies
1
Introduction
Introduction to the Detector 10
Optical System 11
System Overview 14
Leak and Waste Handling 14
Bio-inert Materials 16
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

10 Agilent 1260 Infinity DAD and MWD User Manual
1 Introduction
Introduction to the Detector
Introduction to the Detector
The detector is designed for highest optical performance, GLP compliance and
easy maintenance. It includes the following features:
•80 Hz data acquisition rate for (ultra-) fast LC applications (requires
internal hard disk, G1315C and G1365C only),
•data recovery (DRC) feature provides data-never-lost insurance (requires
internal hard disk, G1315C and G1365C only),
•RFID tags for all flow cells and UV-lamps provides traceable information
about these assemblies,
•long-life deuterium with RFID tag and tungsten lamps for highest intensity
and lowest detection limit over a wavelength range of 190 – 950 nm,
•no loss in sensitivity for up to eight wavelengths simultaneous,
•programmable slit from 1 – 16 nm for complete optimization of sensitivity,
linearity and spectral resolution,
•optional flow-cell cartridges with RFID tag (standard 10 mm13 μL,
semi-micro 6 mm5 μL, micro 3 mm2 μL, 80 nL, 500 nL, 10 mm, high
pressure 10 mm1.7 μL and prep-cells) are available and can be used
depending on the application needs,
•easy front access to lamps and flow cell for fast replacement, and
•built-in holmium oxide filter for fast wavelength accuracy verification,
•built-in temperature control for improved baseline stability,
•additional diagnostic signals for temperature and lamp voltage monitoring,
For specifications, see “Performance Specifications” on page 24.