LI-COR Odyssey CLx User manual

Odyssey® CLx
Operator’s Manual

CE Marking:
This product (model number 9140) is a CE-marked product. For conformity information, contact
LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales
office or distributor.
Doc. #53-15960-A
June 20, 2016
page 1 of 1
Odyssey CLx Imager
Part Name Toxic and Hazardous Substances or Elements
(Pb) (Hg) (Cd) (Cr(VI)) (PBB) (PBDE)
PCBs)
X O O O O O
Electro-
mechanical Parts)
X O O O O O
Optics)
X O X O O O
Cables and Wires)
X O O O O X
(Metal Parts)
X O O O O O
(Plastic Parts)
O O O O O O
(Batteries)
O O O O O O
SJ/T 11364
O = GB/T 26572
(indicates that the content of the toxic and hazardous substance in all the Homogeneous
Materials of the part is below the concentration limit requirement as described in GB/T 26572).
X = GB/T 26572
(indicates that the content of the toxic and hazardous substance in at least one Homogeneous
Material of the part exceeds the concentration limit requirement as described in GB/T 26572).
德CLX

Notes on Safety
LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety
of this product cannot be guaranteed if the product is used in any other way than is specified in this manual. The
Odyssey®CLx Imager is intended to be used by qualified personnel. Read this entire manual before using the
Odyssey CLx Imager.
Equipment Markings:
The product is marked with this symbol when it is necessary for you to refer to the
manual or accompanying documents in order to protect against damage to the product.
The product is marked with this symbol when a hazardous voltage may be present.
Manual Markings:
WARNING Warnings must be followed carefully to avoid bodily injury.
CAUTION Cautions must be observed to avoid damaging your equipment.
NOTE Notes contain additional information and useful tips.
IMPORTANT Information of importance to prevent procedural mistakes in the operation of the
equipment or related software. Failure to comply may result in a poor experimental
outcome but will not cause bodily injury or equipment damage.
Federal Communications Commission Radio Frequency Interference Statement
WARNING: This equipment generates, uses, and can radiate radio frequency energy and if not installed in
accordance with the instruction manual, may cause interference to radio communications. It has been tested and
found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC rules,
which are designed to provide a reasonable protection against such interference when operated in a commercial
environment. Operation of this equipment in a residential area is likely to cause interference in which case the
user, at his own expense, will be required to take whatever measures may be required to correct the interference.
iii

Notice
The information contained in this document is subject to change without notice. LI-COR MAKES NO
WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO
THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.
LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection with
the furnishing, performance, or use of this material.
This document contains proprietary information that is protected by copyright. All rights are reserved. No part of
this document may be photocopied, reproduced, or translated to another language without prior written consent of
LI-COR, Inc.
Printing History
Publication Number 977-19139
Printed April 7, 2019
Revised: May 2020
Global Headquarters
4647 Superior Street • Lincoln, NE 68504
Toll free: 800-645-4267 • Fax: [phone] • [e-mail] •licor.com/bio
Regional Offices
LI-COR Biosciences GmbH
Siemensstraße 25A • 61352 Bad Homburg • Germany
Phone: [phone] • Fax: [phone] • [e-mail]
LI-COR Biosciences UK Ltd.
St. John’s Innovation Centre • Cowley Road
Cambridge • CB4 0WS
United Kingdom • Phone: [phone] • Fax: [phone] • [e-mail]
LI-COR Biotechnology Distributor Network: www.licor.com/bio/distributors
LI-COR is an ISO9001 registered company. © 2020 LI-COR Inc. All rights reserved. Specifications subject to change.
LI-COR, Odyssey, MPX, IRDye, MousePOD, Image Studio, and In-Cell Western are trademarks or registered
trademarks of LI-COR Inc. The Odyssey CLx Imager is covered by U.S. patents, foreign equivalents, and patents
pending.
iv

Chapter 1: Installation and Safety Information
Safety Considerations 1
Laser Safety 1
Safety Interlocks 3
Placement in the Laboratory 3
Ambient Laboratory Conditions 3
Instrument Ventilation 3
Space Requirements 4
Moving the Instrument 4
Chemical Safety 4
Electrical Considerations 5
Power Cords 5
Power Switch 5
Fuse Information 5
Routine Maintenance 5
Networking Cables 6
Chapter 2: System Overview
External Panels and Controls 7
Front Panel and Scanning Surface 7
Rear Panel 8
Scanning Surface 9
General Description of Scanning 9
Continuous Operation 9
Disconnecting the Power 9
Optical System Description 9
Computer Connections and Networking 10
ImageStudio™ Software Overview 11
Learning the Software 11
Chapter 3: Operation
Before You Begin... 13
Cleaning the Scanning Surface 13
Using Membranes 13
Odyssey® CLx
Imager
Table of Contents
v

Using Gels 14
Using Microplates 14
Using the MousePOD Accessory 15
Positioning Membranes and Gels 16
Positioning Microplates 17
Obtaining Technical Support 17
Chapter 4: Appendix
Specifications 19
Imaging System 19
Operating Specifications 19
Embedded Firmware Source Code 19
vi

Chapter 1: Installation and Safety Information
Safety Considerations
Laser Safety
The Center for Devices and Radiological Health (CDRH) was established in October 1982, by the
U.S. Food and Drug Administration (FDA) to protect the public health in the fields of medical
devices and radiological health
Manufacturers of products subject to performance standards under the Radiation Control for Health
and Safety Act of 1968 are required to furnish various reports to the CDRH.
The Odyssey CLx is certified as a Class I laser product. This means that hazardous laser radiation is
not emitted outside the instrument. Radiation emitted inside the Odyssey CLx is confined within
protective housings and external covers. A series of safety interlocks ensures that the laser beam
cannot escape during any phase of user operation.
The CDRH implemented regulations for laser products on August 1, 1976 (CDRH radiation
performance standard 21, Code of Federal Regulations Chapter 1, Subchapter J). Compliance for
products marketed in the United States is mandatory. The label that must be attached to laser
products marketed in the United States is illustrated in Figure 1-2 and is located on the rear panel of
the Odyssey® CLx, indicating compliance with CDRH regulations.
WARNING: Use of controls or adjustments or performance of procedures other than those
specified herein may result in hazardous radiation exposure.
AVERTISSEMENT: L'utilisation des commandes ou le réglage ou la performance des procédures
autrement qu'il a été indiqué dams le présent texte peut résulter en de dangereuses expositions aux
radiations.
The manufacturer label is illustrated in Figure 1-1 and is also on the rear panel of the instrument.
The Odyssey CLx Infrared Imaging System contains two lasers; one emitting at 785 nm, and one at
685 nm. The 685 nm laser emits visible laser radiation - direct exposure to either beam may cause
eye damage. Laser radiation is emitted through apertures at the top of the Odyssey CLx microscope
assembly. Because the microscope assembly moves in both planes relative to the glass scanning
surface, laser radiation could be focused at any position on this surface. Safety interlocks (described
below) automatically turn the lasers off when the Odyssey CLx lid is opened.
The label shown in Figure 1-3 is affixed to the inside of the Odyssey CLx case at two locations; one
near the laser/microscope assembly scanning bed, and the second on the cover that is used to seal
the above mentioned assembly.
Odyssey® CLx
Imager
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Chapter 1: Installation and Safety Information
INPUT
50/60Hz
100-240V 4A
DISCONNECT POWER BEFORE SERVICING
THIS DEVICE COMPLIES WITH PART 15 OF
THE FCC RULES. OPERATION IS SUBJECT
TO THE FOLLOWING TWO CONDITIONS:
(1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE
MUST ACCEPT ANY INTERFERENCE
RECEIVED, INCLUDING INTERFERENCE
THAT MAY CAUSE UNDESIRED OPERATION.
CONFORMS TO UL STD 61010-1
CERTIFIED TO CAN/CSA
STD C22.2 NO. 61010-1
Class 1 Laser Product
Complies with
IEC 60825-1:2014
3rd Edition
DÉBRANCHER LE POUVOIR
AVANT D’ENTRETENIR
THIS PRODUCT COMPLIES WITH CDRH
RADIATION PERFORMANCE STANDARD 21
CFR CHAPTER 1 SUB-CHAPTER J.
CE PRODUIT EST CONFORME À LA
PERFORMANCE DE RAYONNEMENT CDRH
CHAPITRE CFR NORME 1 SOUS-CHAPITRE J.
Figure 1-1. Manufacturer label.
VISIBLE AND INVISIBLE LASER RADIATION WHEN OPEN
AVOID DIRECT EXPOSURE TO BEAM
AVGER SYNLIG OCH OSYNLIG LASERSTRÅLNING NÄR DEN ÄR ÖPPEN
UNDVIK DIREKT STRÅLEXPONERING
SYNLIG OG USYNLING LASERSTRÅLING NÅR ÅBEN
UNDGÅ DIREKTE EKSPONERING AF STÅLE
RAYONNEMENT VISIBLE ET INVISIBLE LASER EN CAS D'OUVERTURE
EXPOSITION DANGEREUSE AU FAISCEAU
DANGER/VARNING/FARE
Figure 1-2. CDRH regulation compliance label. Figure 1-3. Laser radiation danger label.
See "Rear Panel" on page8 for the location of the labels in Figure 1-1 and Figure 1-2. The label in
Figure 1-3 is affixed to the inside of the Odyssey CLx Imager case at two locations; one near the
laser/microscope assembly scanning bed, and the second on the cover that is used to seal the above
mentioned assembly.
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Odyssey® CLx Imager
Safety Interlocks
Interlocks
Figure 1-4. Location of interlocks on the Odyssey® CLx
instrument bezel.
The Odyssey CLx has two safety interlocks that
prevent access to the laser when the instrument
lid is opened during operation. Do not attempt
to defeat these interlocks. The interlocks are
located on the front of the instrument bezel
(Figure 1-4). Audible tones sound when the lid
is opened and closed (Figure 1-4).
Placement in the Laboratory
The Odyssey® CLx Near-Infrared Imaging System weighs approximately 33 kg (72 lbs). The
instrument should be placed on a laboratory bench that is sufficiently sturdy to bear its weight. The
bench should also be able to bear the weight of the computer if the computer will be installed
beside the Odyssey CLx Imager.
Ambient Laboratory Conditions
Place the Odyssey CLx Imager away from external heat sources (furnaces, windows, etc.). Additional
heating can cause high temperatures within the enclosure. Place the instrument away from sinks or
other sources of water that pose a shock hazard. Recommended operating conditions are 15-35 °C
and a dew point not greater than 20 °C to prevent condensation on the laser/microscope assembly
during operation. Allow the instrument to adjust to the temperature and humidity of the room
before powering it on to avoid damaging the instrument. It may be necessary to allow up to a day in
the new location in cases of extreme temperature and/or humidity change.
Instrument Ventilation
The instrument enclosure and circuit boards are cooled with multiple internal fans. The fan on the
rear of the instrument serves as an exhaust port, so the fan shrouds are not filtered and the cover
serves only as an exhaust outlet. There are no restrictions regarding placement of the instrument as
spacers on the rear of the instrument ensure sufficient airflow for the exhaust port. However, it is
important to not allow any objects (e.g., paper or a cover) to block airflow from the exhaust port
while the instrument is on.
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Chapter 1: Installation and Safety Information
Space Requirements
The Odyssey CLx Imager requires an area approximately 37 H x 53 W x 62 D cm (15 x 21 x 24.5
inches). With the hood fully open, the Odyssey CLx requires 74 cm (29”) of vertical clearance.
Moving the Instrument
LI-COR recommends that you do not move your instrument. Please contact your local Technical
Support for LI-COR recommended service options. In extreme situations, you may use the
procedure below to move your instrument.
These instructions are provided as a courtesy for our customers. LI-COR cannot be held
responsible for any damage incurred while moving, transporting, or shipping an Odyssey
CLx Imager, even if an existing warranty and/or service contract applies.
To move your instrument:
1) Ensure the new location meets all requirements specified in Placement in the Laboratory on the
previous page.
2) Turn off the Odyssey CLx Imager by pressing Power on the front panel.
3) Exit Image Studio Software.
4) Be cautious and lift with two people (the instrument weighs about 33kg/72lbs).
5) Lift under the metal enclosure on each side of the unit, keeping the instrument as close to ver-
tical as possible during the move.
6) Gently place the instrument in its new location.
7) Level the instrument to prevent moist membranes from sliding on the scan surface and to keep
the instrument from tilting.
The instrument can be leveled by turning the threaded metal inserts located in each of the 4
plastic feet (Figure 1-5).
Instrument base
Metal insert
Plastic foot
Figure 1-5. Turn the metal inserts on the feet to adjust the height.
Chemical Safety
LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory
procedures be followed at all times. Be aware of the hazards associated with any chemical before you
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Odyssey® CLx Imager
begin work.
The LI-COR Odyssey CLx should not be used with any radioactive materials.
Electrical Considerations
Power Cords
The Odyssey CLx is equipped with a 3-wire grounding-type plug. This plug will only fit into a
grounding-type outlet. This is a necessary safety feature. If you are unable to insert the plug into the
outlet, you will need to replace the outlet. Do not defeat the purpose of the grounding-type plug.
Do not locate the Odyssey CLx where the power cord will be walked on or exposed to water or
chemical spills.
The Odyssey CLx draws at most 4 amperes at 120V. If an extension cord is used, make sure the total
of the ampere ratings on the instruments plugged into the extension cord does not exceed the
extension cord ampere rating. Also make sure the total amperage of instruments plugged into the
wall outlet does not exceed the amperage capacity for the outlet (usually 15 or 20 amperes in the
United States).
To disconnect power before servicing, first disconnect the instrument from the software, briefly
press the power button to power down the instrument, and then remove the power cord from the
power receptacle on the Odyssey® CLx back panel (Figure 1-6). See Disconnecting the Power on
page9 for more information.
Power Switch
A power switch is located above the power receptacle on the back panel (Figure 1-6). Push ‘-‘ to
enable power to the instrument or ‘O’ to disable the power. To power on the instrument, make sure
the power is enabled and press the power button on the front of the instrument.
O
Power Switch
Power Receptacle
Figure 1-6. Location of the power switch and power receptacle on the Odyssey® CLx back panel. Note: The
appearance of your instrument's back panel may vary slightly from the one shown.
Fuse Information
There are no user-serviceable fuses. If the instrument fails to power up, and you suspect that a fuse
has blown, contact LI-COR Biosciences or a LI-COR representative.
Routine Maintenance
The Odyssey CLx requires only minimal maintenance. However, as with any equipment utilizing
electrical voltages, there is a danger of fire or electrical shock if the equipment is not properly
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Chapter 1: Installation and Safety Information
maintained.
LI-COR Biosciences recommends that you routinely inspect the system and the scanning surface.
The following are some general maintenance guidelines:
lWipe all chemical spills from the case and/or scanning surface to prevent damage to the surface
coating.
lInspect all cables and power cords for evidence of fraying, exposed wire, or loose connections.
lKeep the scanning surface free of organic solvents and other combustibles.
lClean the exterior case parts with warm water and a damp cloth. The exterior case parts are
painted with a durable urethane coating that is resistant to chemical spills. Do not use scouring
compounds or solvents (e.g., acetone, benzene, carbon tetrachlorides, lacquer thinner, or alco-
hol) to clean the case.
Networking Cables
LAN
Figure 1-7. Networking
port label.
A Cat. 5e network cable is provided with the Odyssey® CLx Imager. Do not
use networking cables other than the one provided. The use of other
networking cables may result in improper electrical performance. The
networking port is on the back panel of the instrument and is marked with
the label illustrated in Figure 1-7.
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Chapter 2: System Overview
External Panels and Controls
Front Panel and Scanning Surface
Figure 2-1. Front panel features and power button on the Odyssey CLx.
Power On/Off Button: Briefly pressing this button turns the instrument on and off, when the
instrument is not connected to the ImageStudio™ Software. If the instrument is connected,
pressing the button results in the red error light blinking along with a warning sound to alert the
user that the instrument is connected to the software. Continuing to hold the power button for 5
seconds will turn the instrument off and the software connection will end.
Green Image Acquisition Indicator: This indicator light blinks during image acquisition and is
continuously on at other times, as long as communication with ImageStudio is maintained. The
indicator light is off before the instrument is connected to the software and if the Image Studio
Software is closed or communication is otherwise lost.
Odyssey® CLx
Imager
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Chapter 2: System Overview
Figure 2-2. Location of the green image acquisition light and red error light.
Red Error Indicator: The error indicator light illuminates when a particular process could not be
completed.
Rear Panel
Power
switch
Power
receptacle
CDRH
regulation
compliance label
LAN
networking
port
Manufacturer
label
Figure 2-3. Closeup of the Odyssey® CLx Imager rear panel showing the power receptacle and LAN port (use only the
supplied networking cable). Also shown are the CDRH regulation compliance label, the manufacturer label and the
power switch. The power switch can be left in the ‘on’ position. Note: The appearance of your instrument's back
panel may vary slightly from the one shown.
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Odyssey® CLx Imager
Scanning Surface
Width
25
Height
Origin (X,Y)
X-Axis (cm)
Y-Axis (cm)
0
025
Sample
Figure 2-4. Scans progress across the X-axis and
then incrementally move along the Y-axis after each
X-axis pass.
The scanning surface is a 25 cm x 25 cm glass
plate (Figure 2-4), upon which the samples to
be scanned are placed. The scanning surface is
sealed from the instrument interior so no
moisture can penetrate to the detection optics
and electronics below.
General Description of Scanning
Scans are initiated using the ImageStudio Software Acquire ribbon. On the Acquire ribbon the user
chooses the settings for the scan as well as the area to scan. Scanning progresses first across the X-
axis, and then moves incrementally along the Y-axis of the scanning surface after each X-axis pass.
Continuous Operation
The Odyssey CLx is designed to operate continuously. During idle times, the Odyssey CLx can
remain powered on or be powered off at your discretion. When idle, the Odyssey CLx typically
draws approximately 0.5 Amp of current when powered with 120V (see Specifications on page19).
If the Odyssey CLx needs to be disconnected (when moving it, for example), see below.
Disconnecting the Power
First, exit the ImageStudio Software by selecting Instrument > Disconnect on the Application
menu or by closing the application window. Next, briefly press the power button on the front panel
to power off the Odyssey CLx. To disconnect the power before servicing or moving the instrument,
shut down the Odyssey CLx by pressing the power button on the front panel. Then remove the
power cord from the power receptacle as described in Electrical Considerations on page5.
Note: Do not unplug the power cable or push ‘O’ on the rear power switch while the system is
running, except for an emergency situation.
Optical System Description
Solid state diode lasers simultaneously provide light excitation at 685 and 785 nm. Collimating
lenses, optical bandpass filters, and a focusing lens focus and tune the laser beams to produce an
excitation spot on the scanning surface. The microscope electronics then modulate the laser beams
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Chapter 2: System Overview
to discriminate the infrared dye signal fluorescence from background fluorescence. The scanner
detection optics focus on the excitation spot and collect light from the fluorescing infrared dyes.
The light is passed to a dichroic mirror after collection by the microscope objective. The mirror
splits the light and essentially sorts the fluorescent signals by transmitting the light above 810 nm
and reflecting light below 750 nm. Transmitted and reflected light travels two independent paths
through optics designed to remove scattered and stray light. The light is ultimately focused onto one
of two avalanche photodiodes that converts the light to an electrical signal for processing by the
microscope detection electronics.
In the microscope electronics the signal is amplified, filtered, and finally converted to a digital value
by an analog-to-digital converter. The digital signal is demodulated, filtered again, and coordinated
with the microscope position by a Digital Signal Processor (DSP) to produce the image file.
The entire compact laser/microscope assembly travels on a platform that moves beneath the
scanning surface along both the X- and Y-axes. It is adjustable in the Z-dimension to allow for
focusing at the scan bed up to 4 mm above the scan bed to accommodate membranes, gels, and
microplates.
The Odyssey® CLx Imager has two modes for dynamic range, Automatic and Manual. In Automatic
mode the Odyssey CLx acquires images with virtually no saturated pixels on the first attempt with
no user adjustments. More than six logs (22 bits) of dynamic range are available for each image. In
Manual mode the intensity of each channel can be manually adjusted across a 12 bit dynamic
range.
Note: Do not use the Automatic mode when imaging small animals with the MousePOD®
Accessory.
Computer Connections and Networking
Note: Use only the supplied Cat. 5e network cable to connect the Odyssey CLx and computer.
When ImageStudio™ Software starts, it searches for and discovers any Odyssey CLx Imagers
automatically. If only one instrument is found, ImageStudio Software establishes communication.
If more than one instrument is found, the user is presented with a list of instruments and asked
which to use.
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Odyssey® CLx Imager
ImageStudio™ Software Overview
Learning the Software
Each part of the user interface (button, etc.) has an extensive tool tip associated with it that can be
invoked by hovering the mouse over the feature on the user interface. A more comprehensive
review of acquiring images as well as analysis functions can be found in the Image Studio Software
Help system. Updates to the Help system will be available online at: licor.com/support.
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