Porvair Sciences Ultravap Mistral Operator's manual

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V 1.3 – February 2016
U s e r I n s t r u c t i o n M a n u a l
U s e r I n s t r u c t i o n M a n u a l

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U s e r I n s t r u c t i o n M a n u a l
The information in this user guide, including any apparatus, methods, techniques and concepts
described herein, are the property of Porvair Sciences Limited or its Licensees and may not be
copied, disclosed or used for any purpose not expressly authorised by its owners thereof.
This document is for information only; the manufacturer accepts no liability for errors contained
herein or for incidental or consequential damages with the furnishing, performance or use of
this material.
Since Porvair Sciences Limited, constantly strives to improve all of its products, we reserve the
right to modify equipment and user guides without prior notice.
No part of this guide may be reproduced in any form or by any means (including storage and
retrieval or translation into a foreign language) without the prior agreement and written consent
of Porvair Sciences Limited.
Copyright © 2003 – 2014 Porvair Sciences Limited
All Rights Reserved
Specifications subject to change without notice. E&OE.

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Contents
Introduction Page 4
Installation Page 5
Microplate Requirements Page 5
Health & Safety Page 6
Specifications & System Requirements Page 8
What’s in the Box Page 9
Protective Covers Page 10
Operating Instructions Page 11
Connecting the Gas & Power Supply Page 13
Adjustments Page 14
Fitting / Changing Needle Head Page 20
Operational Procedure Page 22
Starting Up Page 22
User Login Page 23
Main Menu Page 24
System Information Page 25
Eco Mode Page 26
Setup Position Page 27
Running a Method Page 29
Master & Slave Page 34
Remote Communications Protocol Page 38
Cleaning Information Page 44
Replacing the Fume Extractor Fan Page 47
Changing Mains Protection Fuse Page 48
Error Messages Page 49
Factory Reset Page 52
Fault Finding Page 53
Identifying Your Ultravap Mistral Page 54
Declaration of Conformity Page 55
Spare Parts & Accessories Page 56
Warranty & Returns Page 57

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Introduction
The Ultravap Mistral has been developed to be an automation friendly, compact, stand alone
blow down sample concentrator, to allow evaporation of solvents, from microplates in minutes,
rather than hours. This instrument encourages integration by incorporating a shuttle style plate
carrier.
The instrument eliminates the traditional “bottleneck” of solvent evaporation prior to analysis or
reconstitution in buffer. It has been designed to be used with ANSI/SBS format 96 well plates
(conforming to the standards mentioned on the next page). The instrument when fitted with
compatible straight or spiral needle heads, which offers improved drying efficiency, can be used
with 96 well plates. All evaporator heads are easily interchangeable. The device will
accommodate either 96 or 384 well plates, with straight needles, or spiralled needles (96 plate
only).
The Ultravap Mistral blows heated gas (typically Nitrogen) into the wells and accommodates both
deep and shallow well plates. The platform containing the well plates, is programmable in
elevation to suit well plate dimensions and dry-down protocol.
The concentrator has been configured to be simple to install, operate and maintain. Installation
requires connection to a gas supply of nitrogen or clean dry air and a suitable electrical supply.
Safe operation is ensured as the CE marked unit fits into fume cupboards, or may be integrated
into a dedicated fume extraction unit.

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Installation
Before use, carry out the following steps:
• Visually inspect Ultravap Mistral for damage
• Check that correct needle head is supplied and inspect for damage
• Ensure transformer is supplied (if required) and a suitable power cord is available
• Ensure that gas and electrical supplies are correct – see Specifications & System
Requirements.
• Place Ultravap Mistral on a flat surface with a fume hood or well-ventilated area
• Connect the Ultravap Mistral to gas and electrical supplies – see Connecting the Gas &
Power Supply.
• Fit the Needle Head – see Fitting / Changing Needle Head.
• Carry out adjustments (if needed) of the Ultravap Mistral – see Adjustments.
Report all damaged or missing components to your supplier or distributor immediately.
The Ultravap Mistral can be used with 96 well microplates that, as a minimum, conform to the
following standards:
ANSI/SBS 1-2004: Microplates – Footprint dimensions
ANSI/SBS 4-2004: Microplates – Well positions
Microplate Requirements

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Risk of Electric Shock
Moving Equipment
WARNING
Refer to accompanying documentation
Earth Protective Conductor
Mains Switch Symbols
DANGER - Hot Surface/Area
Health & Safety
It is Important that the Ultravap Mistral is installed and operated in such a way that all applicable
Heath and Safety requirements are met. It is the users responsibility to ensure that all relevant
Health and Safety Regulations are identified and complied with. Failure to do so, may result in
damage to the equipment and could cause personal injury. In particular, the user should study the
contents of this guide carefully before handling or operating this equipment.
All users should be fully trained and have a full operational knowledge of the equipment.
Under no circumstances will the supplier of this equipment be liable for any incidental, consequential
or any special damages of any kind whatsoever, including but not limited to lost profits arising from,
or in anyway connected with the use of this equipment or this instruction manual.
If the unit is opened or tampered with in any way, the units Warranty is invalidated immediately.
WARNING SYMBOLS in accordance with IEC 417
I - 0
I = ON 0 = OFF
!

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Health & Safety
Environmental Requirements:
Temperature Range: Operating 15⁰C to 40⁰C Storage 0⁰C to 40⁰C
Relative Humidity: Operating 10% to 80% Storage 10% to 80%
Statement: WARNING - Do not operate this instrument in an atmosphere containing explosive gases.
Statement: WARNING - Only approved, supplied mains cord must be used with this instrument.
Statement: WARNING - If an extension lead is required, the lead MUST be earthed.
Statement: VOLTAGE – The Ultravap Mistral is supplied for direct connection to normal 115VAC
or 230VAC supply, with a variation in supply that gives a voltage range of 108VAC to 250VAC.
• The provided covers MUST be used at ALL times during the operation of the unit.
• At no time should the plates be inserted whilst the unit is in its application state.
• The use of solvents on the unit is not recommended.
• The air supply must be removed and the electrical supply disconnected, prior to the removal of any
covers.
The Mains Plug supplied with the Ultravap Mistral unit, is fitted with the following fuse:
Only refit the correct type of Fuse. Must be IEC127 approved for use in EC Countries.
Must be C.S.A. or UL listed or recognised for use in Canada or the United States of America.
Porvair Sciences Limited accepts no responsibility for the misuse of this equipment.
Supply Fuse fitted in plug Fuse fitted in MiniVap
230VAC U.K 13 Amp IEC Main inlet to rear of
unit is twin fused, Live &
Neutral fuse. (2 x T5AH
250V)
230VAC Europe No fuse
115VAC No fuse

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Power Supply: 115 - 240 - V AC 50/60Hz
Fuse Rating: 2 x 5 Amp Anti-surge (T5AH 250V)
Dimensions: 180mm x 480mm x 450mm (W x H x D)
Weight: 17.5 Kg
Gas Input: 8mm Inlet
Min Operating Pressure: 4 bar (60 psi)
Max Operating Pressure: 7 bar (100 psi)
Flow: 30 L/min -100 L/min
Min Operating Temp: 15⁰C
Max Working Temp: 40⁰C
Protection: IP 40
Specifications & System Requirements
Optimum Conditions
Pressure: 5.5 bar (80 psi)
Flow: 70 L/min
Gas Supply Information
A suitable clean, dry, regulated gas supply should be connected to the instrument using 8mm
tubing. Use of a filter / separator in addition to supply regulator is strongly recommended. Typical
gases suitable for use with the Ultravap Mistral are Nitrogen and compressed air.
For samples that are easily oxidised it is strongly recommended that Nitrogen is used. The gas can
be supplied from cylinders/bottles, via in-house systems, local compressor (air) or from a gas
generator (Nitrogen).

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What’s in the Box
Before installing your Ultravap Mistral, please check that all the required contents are in the
box. See below for a full list of items.
1 x Ultravap Mistral Unit
1 x UK Power Lead
1 x EU Power Lead
1 x 4mm Allen Key
1 x 3mm Allen Key
1 x Stubby Flathead Screwdriver 1 x US Power Lead
1 x Touch Screen Stylus

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Protective Covers
Prior to using your Ultravap Mistral, ensure protective films and packaging are all removed:
Remove screen
bezel protecting
foam pad
Peel off screen
protector film
Remove safety
guard green
protective film
(remove splash
guard first)

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Operating Instructions
The Ultravap Mistral has been designed to assist the use of integrating; please take note of the
dimensions below for accommodating the size of the unit. All dimensions are in mm.
Note: The Ultravap Mistral features a safety function for the shuttle travelling out. It includes a “bump”
action stop in case an obstruction is detected. Once the safety switch is activated, the procedure will
be paused and an error message will appear. The carrier can be homed once the error has been
cleared on the display. See Error Messages for details.

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Operating Instructions
Safety / Splash Guard
Moving Shuttle with
Plate Carrier
Fume Extractor Touch Screen Display
Touch screen stylus
(clipped to back of
screen bezel)

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Connecting the Gas & Power Supply
Connecting the Gas Supply
Check that the gas supply is within the system requirements.
The gas supply should be connected to the universal connector female 8mm (internal diameter) on
the rear of the unit. The connecting tube is a one-touch connection.
Gas pressure should not exceed 7 bar (100 PSI). Exceeding this pressure may damage the unit.
Connecting the Power Supply
The Ultravap Mistral utilises a standard Euro inlet, which is fused and switched prior to inserting
the connector. Check that the correct voltage is being applied.
Air Supply
Inlet (8mm)
CAN
Connector
(Female)
Mains
Inlet
RS232
Connector
(Male)

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Adjustments
BEFORE USING ULTRAVAP MISTRAL CARRY OUT THE FOLLOWING STEPS:
When unpacking your Ultravap Mistral unit, visually inspect the needle array for correct pitch.
• 96 well pitch (distance between pins) should be 9.00mm
• 384 well pitch should be 4.50mm
This maybe checked by offering up a standard 96/384 well plate.
Check visually, that no obvious damage has occurred during shipping.
Once you have correctly followed the instructions on connecting Ultravap Mistral to gas and
power supplies, the unit is ready for use.
96 Well Head 384 Well Head

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Safety guard screws
Adjustments
Removing the Safety Guard
The Ultravap Mistral features a safety guard to protect users from potential pinch points between
the shuttle and the needles. However the safety guard can be removed by taking out the screws
either side with the flat head screwdriver provided.

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Adjusting the Shuttle Carrier
The Ultravap Mistral has an adjustable carrier, which can be moved for microplate location. They
are set up to fit Porvair Sciences Limited deep well plate as standard.
• Adjustment is carried out by loosening the location screws, positioning the plate and re-
tightening the screws.
• Check that the needles are centrally located above each well. If the plate is not located
properly, move the carrier to correctly align the plate.
Note: The carrier can be driven manually to a desired location in Position Setup, under the
services menu, see Operating Procedure.
Adjustments
Screws to loosen for adjustment
Direction of
adjustment

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Adjustments
Adjusting the Screen
The touch screen featured on the Ultravap Mistral can be adjusted by hand for optimum viewing,
also the screen can be folded down for protection during transportation of the instrument.
Axis of Screen Rotation
Screen Knob
Screen Folded Down

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Adjustments
Adjusting the Screen
Another feature of the adjustable screen on the Ultravap Mistral is that it can be turned around to
face backwards; this can be done to assist the user during integration. (For example when the
instrument is faced against a machine it can be difficult to reach the screen).
To turn the screen around follow these steps:
• Disconnect any power to the instrument
• Unplug the black connector from the back of the screen bezel
• Loosen and remove both of the screen knobs
• Turn the screen bezel around to face the opposite way
• Put the screen knobs back in and tighten up enough to support the screen bezel
• Plug the black connector back in to the screen bezel without twisting (fits both ways round)

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Adjustments
Adjusting the ScreenAdjusting the Screen

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Fitting / Changing Needle Head
Before removing / changing the needle head, disconnect the unit from any electrical
supplies and gas. Also ensure Ultravap Mistral has cooled down sufficiently if used.
• Remove the safety guard.
• To remove the needle head, remove the two fixing screws, using the Allen key provided.
Turning the unit on it’s side may make this process easier (ensure it is placed on a suitable
surface and a cloth is used if necessary for protection).
• Carefully remove the head, avoid bending or damaging needles.
• Inspect gasket for tears and replace if needed.
• When replacing the needle head, great care must be taken to ensure that the gasket is
present and fitted correctly. Also, ensure that the needle head locates on the dowels and the
opposing corner screws are used so that the needle head is fixed evenly. For ease of fitting;
the needle head will fit either way round.
Fixing screw
Fixing screw