premiere MRJ-01 User manual

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PROFESSIONAL MICROSCOPE
Model:
MRJ-01 - Monocular
MRJ-03 - Binocular
MRJ-03D - Dual View
MRJ-03T - Trinocular

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Contents MRJ
1. Before use…………………………………..………………..……3
2. Diagram…………………………………….……………...………4
3. Assemble………………………………………………..…………6
4. Operation……………………………………………...…………...6
5. General specification………………………...………………..…10
6. Configuration drawing……………………………….…………..11
7. Specification………………………………………..……………12
8. Troubleshooting…………………………………...……………..13
9. Trinocular / Dual View microscope……………...………………15
10.Warranty………………………………………………………….15

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1. Before use MRJ
1-1. Operation
(1) As the microscope is a precision instrument, always use it with care. Avoid dropping or shaking during
operation. Make sure the microscope is placed on a flat surface.
(2) Do not put the microscope in direct contact with the sun. Do not expose the microscope to high temperature,
damp, or dusty environment.
(3) When moving the microscope, you should use both hands, typically one hand under the base and one hand on
the arm (stand) of the microscope and set it down carefully.
★It will damage the microscope if you hold the microscope either by the stage, focusing knob or head.
(4) Make sure the microscope is unplugged before replacing the bulb or fuse and wait until the bulb has cooled
down.
★Lamp selected only：6V/20W Halogen Bulb
(5 ) This microscope has a built in dual voltage transformer and can be used with wide voltage range from 90 to
240V.
(6) Use only electrical plug supplied with the microscope.
1-2 Maintenance
(1) Wipe the lens gently with a soft lens tissue. After every use with oil or fingerprints clean the lens surfaces with
lens paper or lens paper moistened with a little rubbing alcohol. If you do not have lens paper make sure to
use a lint free towel or cloth.
★ Alcohol is flammable. Do not place this chemical near fire or fire causing source. You should use this
chemical in a ventilated place.
(2) Don’t use organic solution to wipe the surfaces of the other components.
(3) If the microscope comes in contact with a liquid, then you should turn off the power immediately and wipe it
dry.
(4) Never disassemble or service the microscope yourself.
(5) After using, cover the microscope with a dust cover.

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2. Diagram MRJ
MRJ Intermediate Biological Microscope Diagram
Eyepieces
Head lock screw
Arm
Head
Nosepiece
Objectives
Stage Stop
Stage
Course Focus
Condenser
Light
Variable light
control knob
Fine Focus
Condenser
up-down
knob

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2. Diagram MRJ
MRJ Biological Microscope Diagram
Course Focus
Fine Focus
Mechanical Stage
X-Axis knob
Condenser
Y-Axis knob
Condenser
up-down
knob
Condenser center
adjustment screw
Aperture Diaphragm

3. Assembly MRJ
3-1 Install the Objectives
These have been factory installed.
3-2 Insert the Eyepieces
(1) Remove the eyepiece tube covers.
(2) Insert the eyepiece into the tube completely.
4. Operation MRJ
4-1 Turn on
(1) Insert the plug into an outlet and turn on.
(2) Adjust the light adjustment knob until you get the
suitable brightness. (See fig.1)
Fig.1
4-2 Set the specimen slide
(1) Push the arm of the specimen holder back.
(2) Insert the slide with the cover glass facing up
and allow the arm of the specimen holder forward.
(Seefig.2)
(3) Rotate the X and Y-axis knobs on the mechanical stage to
movethespecimentothecenter(thecenterofthe
objective).
6

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4.Operation MRJ
4-3 Adjust focus
图3
Fine-focusing
-knob
Course Focus
knob
(1) Move the objective 4X into the light path.
(2) Rotate the coarse-focusing-knob until the image
appears in focus.
(3) Turn the fine-focusing-knob to make the specimen
clearer. (See fig.3)
When using the 4X and 10X objective, open
the aperture diaphragm and field diaphragm
to the widest position.
Fig.3
.
Eye distance indicate board
4-4 Adjust the interpupillary distance
When observing with both eyes hold the bases of the base
of the eyepieces and rotate them around the axis until there
is only one field of view. (See fig.4). On the left and right
eyepiece base should point to the same number. The
number is the interpupillary distance.
Fig.4
4-5 Adjust diopters
Rotate one eyepiece to the “0” position using the white
thread. This should be done to your dominate eye, then
using that same (one side only) eyepiece focus on an
specimen clearly. Now observe the other eyepiece and
rotate its diopter adjusting ring until the image is clear in
that eyepiece. (See fig.5)
Rotate Diopters
Fig.5

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4. Operation MRJ
4-6 Center the condenser
(1) Rotate the condenser up-down knob ①to raise it to the
highest position. (See fig.6)
①
②
③
(2) Rotate the 4X objective into the light path.
(3) Rotate the Aperture Diaphragm ②in a counterclockwise
direction and move to the smallest setting. and you will see the
image of field diaphragm from the eyepieces.
(4) You want to see the circle of light closest to the center. If
is centered go to section 4-7, if not go to the next step
it
.
Fig.6
Image of aperture diaphragm (inside)
(5) Adjust the center adjustment screw ③and put the light
to the center of the field of view [Make sure the aperture
diaphragm is smaller than the field of view of the eyepiece
(See fig.7). When the shadow around the field of view of the
eyepiece is symmetrical, it shows the condenser has been
centered correctly. (See fig.7)
(6) Now, you can enlarge the aperture diaphragm ②and
remove the shadow.
Fig 7
Outside
edge of
objective
4-7 Adjust aperture diaphragm ②
The aperture diaphragm decides the numerical aperture of the
illumination. If the N.A. of illumination is matching with N.A.
of the objective②, you can obtain better resolution and the
contrast.
If the contrast is low, you can adjust the N.A. of the condenser
to 70%-80% of the N.A. of the objective when using. Adjust
the upper diaphragm ring until you see the image like above
(See fig.8). Use of diaphragm and put the N.A. of condenser
Fig 8
②to 80% of the objective. e.g.: objective 40X (N.A. =0.65),
put the aperture diaphragm to about 0.65X0.8=0.52. (See
fig.9)
Fig.10
Switch to open and
close Aperture
Diaphragm
Fig 9

4. Operation MRJ
4-8 Use the oil objective
(1) Use the objective 40X to focus the specimen.
(2) Put a drop of oil on the specimen. (See fig.10)
(3) Rotate the nosepiece to place the 100X objective (oil) into
the light path. Then use the fine focusing knob to focus.
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★Make sure there is no air bubble in the oil.
A. Take out the eyepieces to examine the air bubble. Open the
aperture diaphragm and field diaphragm fully and observe
the edge of the objective from the tube without the eyepiece.
Fig.10
Oil position
B. You can rotate nosepiece slightly and swing the oil
objective some times to remove the air bubble.
(4) After using oil, clean the 100X lens with clean lens paper
using rubbing alcohol if the oil does not come off easily.
4-9 Use the filter
Filter can make the background softer. (fig.11)
Filter holder
Thereisafilters:blue.
(You also place the Polarizer in this position when using the
optional simple polarizing set)
Fig.11
Fig.12
4-10 Replace the halogen bulb
(1)
Turn off the power supply before replacing the bulb.
(2) Loosen the screw of the lamp holder base, and open the
door to the lamp holder.
(3) Wait until the bulb cools down, and then insert the new
bulb into the bulb holder on the lamp base.
(4) Close the door of the lamp holder and retighten the screw
(See fig.12)
4-11 Replace the fuse
Turn off the power supply before replacing the fuse. On the
bottom screw off the fuse holder from the base with a “flat head”
screwdriver. Remove the damaged fuse and install the new fuse.
Finally screw the fuse base back to the base. (See fig.13)
Fuse base
ReplacementFuse-Rating:250V，3.15A.
Fig.13

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5.General specification MRJ
MRJ Biological Microscope general specifications (For optional upgrade please
call your authorized dealer)
Install Model
Parts fications MRJ-01
MRJ-03 MRJ-03T MRJ
Speci -03D
10X/18mm eyepiece 1 pcs 2 pa
••ir
PL16X plan eyepiece
Eyepiece
(pair) Center eyepiece
Achromatic objective 4X • •
• •
Achromatic objective 10X ••••
Achromatic objective 40X ••••
Ac ctive 100X •• hromatic obje ••
Plan achromatic objective 4X
Plan achromatic objective 10X
Plan achromatic objective 40X
Objective
Plan achromatic objective 100X
45” Monocular Head •
30 ocular head °Bin •
30 lar head °Trinocu •
Head
30°Dual Binocular head •
Nosepiece Rev ple nosepiece ••
ersed quadru ••
Stand C••••
Stage 140 x 135mm mechanical stage ••••
N.A.1.25 abbe condenser ••••
N.A.1.25 five position phase
Si ase contrast ngle special ph
Single special phase contrast
Single special phase contrast
Power
90 ide voltage
6V/20W halogen bulb (built in)
Variable ontrol ad
V-240Vw
c
j
ustment
• •
• •
Blue filter(φ32 ••••
Spare bulb ••••
Accessory
Replacement fuse ••••
Dry dark filed condenser
Dark field
condense
r
Oil dark field condenser
Polarizer(φ32)
Analyzer
Polarizing
set Rotating stage (center)

6. Configuration drawing MRJ
MRJ Biological Microscope Configuration drawing
11
0.35X TV adapter
Trinocula
r
Bod
y
Center Eyepiece
15X Eyepiece
Filte
r
Polarize
r
Binocular
10X Eyepiece
N.A.1.25 Oil dark field condenser
Blue Green
Mechanical moving stage for left
N.A.0.9
Dry dark field condenser
N.A.1.25 Abbe achromatic condenser
Phase contrast ob
j
ective
Achromatic ob
j
ective
N.A. 1.25 Phase contrast condenser

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7.Specification MRJ
7-1 MRJ Biological Microscope general specifications
Optical Achromatic Optical system
Head
Binocular, 30°inclined.
Trinocular, 30°inclined.
45°Dual Binocular head
Eyepiece 10X eyepiece, line field of view 18mm
Nosepiece Reversed quadruple nosepiece
Objective Achromatic objective(4X、10X、40X、100X)
Plan objective(4X, 10X, 40X, 100X) (not included)
Focus Coaxial coarse & fine focusing system with limit-stopper and tension adjustment. Travel
range: 20mm. Fine focusing precision：0.002mm
Stage Mechanical stage 140x135mm; moving range 75mmx50mm; built in low position coaxial
adjustment, left and right hand for option.
Condenser
N.A.1.25 achromatic condenser
N.A.1.25 five position phase contrast condenser (not included)
N.A. 0.9 dry dark field condenser (not included)
N.A. 1.25 oil dark field condenser (not included)
Illumination 90～240V wide voltage. Built-in Koehler illuminator system. 6V/20W halogen bulb.
Pre-centered, a continuous adjustment of brightness.
7-2 Parameter of objective
Type Magnification
Numerical
aperture
（N.A.）
Working
distance
（mm）
Conjugate
distance
（mm）
Parfocal
distance
（mm）
Thickness of
the cover
glass
4x 0.1 17.912 195 45 0.17
10x 0.25 2.043 195 45 0.17
40x 0.65 0.65 195 45 0.17
Plan objective
100x 1.25 0.16 195 45 0.17
4x 0.1 17.912 195 45 0.17
10x 0.25 2.043 195 45 0.17
40x 0.65 0.65 195 45 0.17
Plan objective
100x 1.25 0.16 195 45 0.17

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8. Trouble shooting MRJ
Symptom Cause Remedy
Optics
Field diaphragm is not large enough. Enlarge the field diaphragm.
Condenser is too low. Adjust the condenser.
(1)The lamp is bright,
but the light in the
field of view is not
bright enough. Condenser is not centered. Center the condenser.
The nosepiece is not in the right position. Turn the nosepiece into the right
position.
Stain or dust has accumulated on the condenser,
objective, eyepieces, and base lens. Clean the lens.
(2)The side of the
field of view is dark or
not even.
Condenser adjustment needed (see step 3-7) Center the condenser.
Stains have accumulated on the specimen. Clean the specimen.
(3)Stain or dust is
observed in the field
of view. Stains have accumulated on the lens. Clean the lens.
No cover glass on the specimen slide. Add the cover glass.
The cover glass is not standard. Use a standard cover glass with
thickness 0.17mm.
The cover glass faces down. Put the cover glass to face up.
The immersion oil has accumulated on the dry
objective. Clean thoroughly.
The immersion oil is not used for oil objective
100XR. Use immersion oil.
Air bubble in the immersion. Get rid of the air bubble.
Use wrong immersion oil. Use correct one.
The aperture is not opened correctly. Adjust the iris diaphragm.
Stain or dust has accumulated on the lens in the
inlet of the head. Clean the lens.
(4)Unclear image
The condenser is not in the right position. Adjust the condenser.
The specimen slide is not fixed. Fix with clips.
The nosepiece is not in the right position. Turn the nosepiece into the right
position.
(5)One side of the
field of view is dark or
the image moves
while focusing. Condenser is centered incorrectly. Center the condenser.
Interpupillary distance is wrong. Adjust the interpupillary distance
Diopter adjustment is wrong. Adjust the diopter.
(6) The eyes feel tired
easily. The right field
of view doesn’t
superpose with the
left. The eyepiece for the right is different from the
left. Use the same eyepieces.

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8. Trouble shooting MRJ
Symptom Cause Remedy
Mechanics
The cover glass faces down. Put the cover glass to face up.
(1)Can not get the
objective focused. The cover glass is not standard. Use a standard cover glass with
thickness 0.17mm.
The cover glass faces down. Put the cover glass to face up.
(2) The objective
touches the cover
glass while turning
the nosepiece. The cover glass is not standard. Use a standard cover glass with
thickness 0.17mm.
(3)Coarse focusing
knob is too tight. Tension knob is too tight. Loosen a little.
(4)Stage declines
itself and can’t stay in
the focus plane.
Tension knob is too loose. Tighten a little.
(5)Coarse focusing
knob can’t rise. The limit stop knob is locked. Loosen the knob.
(6)Coarse focusing
knob can’t decline. The base of the condenser is too low. Raise the base.
The slide is not fixed correctly. Adjust it correctly.
(7)Can not move the
slide smoothly. The movable specimen holder is not fixed
properly. Adjust it correctly.
(8)The image moves
obviously when
touching the stage.
The stage is fastened incorrectly. Fasten the stage correctly.
Electrical
No power supply. Check the connection of the
power cable.
The bulb is not inserted correctly. Insert it correctly.
(1)The bulb does not
work.
The bulb burnt out. Replace it.
(2)The bulb burnt out
usually. Use a wrong bulb. Replace with a correct one.
Use a wrong bulb. Replace with a correct one.
(3)The field of view is
not bright enough. The use of light adjustment knob is wrong. Adjust correctly.
The bulb will burn out soon. Replace with a new one.
(4)The bulb flickers
or the brightness is
not stable. The wire doesn’t connect all right. Connect correctly.

MONOCULAR / TRINOCULAR / DUALVIEW
MICROSCOPE
Monocular – Can be fitted with a monocular for a economical price
Trinocular - The Trinocular head fits on the base in place of binocular head, third 10X eyepiece
(not included) may be used or camera eyepiece (sold separately) can be inserted in the vertical
tube for video monitor use or digital images with your computer. (Ask for MA87 or MA88).
Dual View - MRJ microscopes may be adapted to function as a Dual View microscope with front
and back viewing.
MRJ-01 MRJ-03D MRJ-03T
Warranty
The manufacturer warrants this instrument to be free from defects in material and workmanship under normal use
for five years from the date of purchase (one year for electrical components). It does not cover damage resulting
from abuse or misuse, repairs or alterations performed by other than authorized repair technicians, or damage
occurring in transit. If you have questions concerning this product or warranty, contact the dealer from whom it
was purchased. For warranty service, microscope should be well packed to avoid damage in transit, preferably in
original box and packing. Include your complete return address and telephone number as well as a description of
the difficulty, date and place of purchase, and ship to the address below. It will be repaired or replaced at no charge
and returned. If misuse, alterations, accident or abnormal conditions of operation caused failure, an estimate for
repairs will be provided for your approval prior to work being performed.
(8/3)
C & A Scientific Co., Inc.
7241 Gabe Ct ●Manassas ●Virginia ●20109
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