Prior Scientific OptiScan II ES111 User manual

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Motorized Microscope Stages
Operating Instructions
OptiScanII
™
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OptiScan II™1
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1. Important Safety Information 3
2. Unpacking The System 7
2.1 Other Accessories 10
3. Installation 11
3.1 Removing an Existing Stage 13
3.2 Fitting the OptiScan II Stage 13
3.3 Cable Connections 14
3.4 Focus Drive Installation 15
3.5 Installing Filters 17
3.6 Mounting Filter Wheels and Shutters 18
4. Getting Started 19
4.1 USB Operation 22
4.2 Using The Joystick (CS152DP) 24
4.3 Using the Z Axis Digipot (CS152Z) 25
4.4 Using the Filter Wheel Keypad (CS100K) 25
4.5 Touch Screen Keypad (CS152KB) 27
5. Advanced Operation 29
5.1 RS232 Command Set 31
5.2 Macro and Soak 33
5.3 Soak Command 33
5.4 General Commands 34
5.5 Stage Commands 37
5.6 Z Axis Commands 42
5.7 Filter Wheel Commands 45
5.8 Shutter Commands 46
5.9 Pattern Commands 47
5.10 H127/H128 Compatible Commands 49
5.11 Z Axis Encoder 52
5.12 Error Codes 54
5.13 CS152 (Joystick Configuration) 55
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2Prior Scientific
6. Troubleshooting 57
7. System Specifications 63
8. Returns and Repairs 67
9. Appendices 71
9.1 Non Standard Focus Drive Installations 73
9.2 Direct Coupling Focus Installation 80
9.3 How to Run HyperTerminal 83
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OptiScan II™3
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OptiScan II™5
Important Safety Information
•Keep this manual in a safe place as it contains important safety information and operating
instructions.
•Before using the stage system, please follow and adhere to all warnings, safety and
operating instructions located either on the product or in this User’s Manual.
•Do not expose the product to water or moisture.
•Do not expose the product to extreme hot or cold temperatures.
•Do not expose the product to open flames.
•Do not allow objects to fall on or liquids to spill on the product.
•Connect the AC power cord only to designated power sources as marked on the
product.
•Make sure the electrical cord is located so that it will not be subject to damage.
•Always disconnect power from product before connecting the components
together.
•DANGER - never alter the AC cord or plug. If the supplied plug adaptor is not the
correct fitting for your geographical area, contact your supplier for the correct power
adaptor.
•Use only the power supply cord set provided with the system for this unit, should this
not be correct for your geographical area, contact your supplier.
•Do not in any way attempt to tamper with the product, doing so will void the
warranty, and may damage the system. This product does not contain consumer
serviceable components, all repairs or servicing should be performed by Authorised
Service Centres, contact your local dealer for details.
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OptiScan II™7
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8Prior Scientific
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OptiScan II™9
Unpacking The System
The OptiScan II System consists of the OptiScan II Controller and one or more of the
following components A-G.
Component Component Description
A Model CS152Z Digipot, Z only
B Model ES111 Stage for Upright Microscopes includes 15 pin cable
C Model ES107 Stage for Inverted Microscopes includes 15 pin cable
D Model H122 Focus Drive
E Model H122AXIO Focus Drive
F Model CS152DP Joystick, 2 axis with digipot
G Model HF110 25mm, 10 position Filter Wheel for Microscopes includes
15 pin cable or Model HF108 32mm 8 position Filter Wheel for
Microscopes includes 15 pin cable.
H Model ES10 or ES10E Controller.
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10 Prior Scientific
Other accessories such as specimen holders may be included with your OptiScan II system.
You will find a list of the most frequently supplied accessories in section 3.1 under “Other
Accessories”.
2.1 Other Accessories
Model Number Description
CS100K Keypad for Manual Control of 2 Filter Wheels and 3 Shutters
CS152KB Touch Screen Keypad
H122KLC Direct Coupling Adapter for Focus Drive on Leica
H122KON Direct Coupling Adapter for Focus Drive on Olympus/Nikon
H276K RS232 cable for PC (9 or 25 pin)
H277 RS422 cable for Macintosh (8-pin Mini Din to 9 way D Type)
HF200 High Speed Shutter, 25mm aperture for use when mounting to
filter wheel
HF202 High Speed Shutter, 25mm aperture (for stand alone use)
HF201 High Speed Shutter, 32mm aperture for use when mounting to
filter wheel
HF204 High Speed Shutter, 32mm aperture (for stand alone use)
HF205 Dual Slide in Filter Holder for 25mm Filter Wheels
HF206 Dual Slide in Filter Holder for 32mm Filter Wheels
HF207 Male to Male C mount adapter
HF210 Filter Wheel Adapter for Leica pre DM Series
HF215 Filter Wheel Adapter for Leica DM Series
HF220 Filter Wheel Adapter for Nikon Eclipse Series
HF230 Filter Wheel Adapter for Olympus BH Series
HF235 Filter Wheel Adapter for Olympus BX, IX and AX Series
HF240 Filter Wheel Adapter for Zeiss Axio Series
HF245 Filter Wheel Adapter for Zeiss pre Axio Series
HF535 Mounting Plate (Converts HF115 into HF110)
HF559 Cover Plate (Converts HF200 into HF202)
Note: Make sure that all of the components that should be included with your OptiScan II System
have been supplied. If parts are missing, please contact your local Prior Dealer.
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OptiScan II™11
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OptiScan II™13
Installation
3.1 Removing an Existing Stage
To avoid damage to the optics when removing an existing stage, ensure that the distance
between the objectives and stage is maximised, and that the condenser is clear of the stage.
Removal of the stage is normally a straightforward procedure, in most cases just by the
removal of fixing screws or the loosening of a clamp screw.
3.2 Fitting the OptiScan II Stage
The Prior OptiScan II stage is supplied with a base plate to suit the microscope specified.
Place the stage onto the microscope stage mount and attach using the fixing screws or
clamping screw supplied. Confirm that the OptiScan II controller unit is switched off before
connecting the stage to the controller with the lead provided (see fig.4.3).
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14 Prior Scientific
3.3 Cable Connections
The cable connections to the OptiScan II controller are located on the rear panel of the
control box, as shown in the illustration below. Before making any of these connections,
ensure that the OptiScan II controller is switched off. Each connection is well labelled. Do not
attempt to connect your computer's serial port cable to the ` Z' axis connector on the
controller, this may damage your computer and the OptiScan II controller.
The RS232 connection from your computer should be made to the RS232-1 port on the
controller.
Figure 4.3
RS 232 (1) Connection
RS 232 (2) Connection
Power Input
USB Socket
J
o
y
stick/Di
g
i
p
ot
Z-Axis
XY Sta
g
e
Filter Wheel 1
Filter Wheel 2
Z- Encoder (Not Fitted)
Shutter 1
Shutter 2 Shutter 3
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OptiScan II™15
3.4 Focus Drive Installation
For installation procedure for Zeiss Axio range (H122AXIO and H122AXIE),
Leica DML range (H122LB), and direct coupling models, see Appendix A.
The following instructions refer to the standard split sleeve mounting.
1. Loosen the clamp screw on the focus motor assembly and remove the focus motor from
the focus adapter. (See fig.4.4)
2. Loosen the 3 socket set screws around the periphery of the focus adapter using a 2mm
Allen wrench until the focus sleeve is able to fit inside the adapter. Note that it is important
to insert the sleeve in the correct orientation with the lip furthest inside the adapter (the
chamfered edge of the sleeve will be inserted first). Note the orientation of the sleeve as it
has a recess around its outer surface, which will hold the sleeve in when the setscrews are
tightened. This recess must line up with the tips of the socket set screws. (See fig.4.4).
3. With the sleeve in place, tighten the 3 socket set screws in sequence until they all just
touch the sleeve, ensuring that the split in the sleeve does not line up with any of the set
screw positions. DO NOT TIGHTEN UP ANY OF THE SETSCREWS AT THIS STAGE.
4.
Push the adapter onto the preferred coarse knob of the microscope as far as it will go.
The controller is factory configured to drive the focus motor in the correct
direction when mounted to the right hand side of an upright microscope. If the
left hand coarse control knob is preferred by the user or the focus drive is to
be mounted on an inverted microscope, the motor direction can be reversed
by using a PC with a terminal emulation programme e.g. HyperTerminal and
changing the settings of the ZD command (see section 6) via RS232
communication.
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16 Prior Scientific
The inside fitting diameter of the sleeve is designed to be slightly larger than the coarse knob,
provided the setscrews have not been tightened and are compressing the sleeve.
5. While holding the adapter in place, tighten the set screws in sequence only enough to
secure the unit onto the coarse focus knob. The focus knob will have to be rotated to gain
access to all of the screws.
6. Check that the unit has been tightened sufficiently by taking hold of it and turning it. If the
adapter is correctly fitted it will stay attached to the coarse knob.
7. Slide the focus motor into the adapter as far as it will go and while applying gentle pressure
to the motor, tighten the clamp screw. This will hold the motor in place. The rubber drive
bush on the end of the motor spindle should now be pressing against the end surface of the
fine focus control knob. This can be confirmed by manually rotating the exposed fine focus
knob on the opposite side of the microscope and feeling for the resistance caused by the
detent positions of the stepper motor as it rotates. This will not cause any damage to the
focus motor.
8. Confirm that the controller is switched off before connecting the 9 way D type plug on the
focus motor lead to the socket on the rear of the controller as shown in fig.4.3.
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OptiScan II™17
3.5 Installing Filters
It is recommended that filters are installed before mounting the filter wheel on to a
microscope.
1. Select filter position required for loading, by checking the number displayed in the load
position indicator window (A) (see fig.4.5).
2. Remove the magnetic covers (B) from the load port.
3. Remove lock ring (C) by unscrewing from the filter holder cell with the tool D
provided.
4. Insert desired filter and replace lock ring.
5. Repeat this process for all the desired filter positions.
6. Replace magnetic covers.
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