Qiagen FLUO SENS User manual


Manual

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FLUO SENS integrated Manual
January 2013
Notice
All rights reserved.
The information contained in this document is subject to change
without notice.
Reproduction or republication of this document in any form or format is
prohibited without the written consent of QIAGEN Lake Constance
GmbH.
Trademarks
Trademarks and labels used are the property of their respective
owners.
Copyright Information
User Manual for FLUO SENS integrated
Document Number ESMO30-DH-1001
Document Revision 05 / January 2013
© Copyright 2009/2012 by QIAGEN Lake Constance GmbH
Printed in Germany
QIAGEN Lake Constance GmbH
Jacques-Schiesser-Strasse 3
D-78333 Stockach
Germany
Tel: +49 (0) 7771-9166-0
Fax: +49 (0) 7771-9166-218
Email: in[email protected]m
Website: www.qiagen.com
QIAGEN Lake Constance GmbH is certified according to
ISO 9001 and EN 13485.

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Table of Contents
1General Product Description............................................. 4
2Technical Specifications ................................................... 5
3Connections ....................................................................... 6
4Physical Dimensions ......................................................... 7
4.1 ESMO40-MB-xxxx Detector .................................................7
4.2 ESMO30-MB-xxxx Detector .................................................7
5Operating Conditions......................................................... 8
5.1 Environment.........................................................................8
5.2 Safety Instructions...............................................................9
6Contents of the FLUO SENS integrated Package.......... 10
6.1 Start up...............................................................................10
6.2 Mounting ............................................................................10
7Establishing Connection with a Computer .................... 11
7.1 Connecting a Single Detector ...........................................11
7.2 Connecting Multiple Detectors .........................................11
8Instructions for Usage in Compliance with EMC-
Directive 89/338 EWG ............................................................. 12
8.1 EMC-Safety Precautions ...................................................12
8.2 Switching on the Device....................................................12
8.3 Switching off the Device ...................................................12
9Operation .......................................................................... 13
9.1 MODBUS-ASCII ..................................................................13
10 Configuring a MODBUS-Protocol ................................ 14
10.1 Longitudinal Redundancy Check (LRC)...........................14
10.2 MODBUS Function Codes .................................................14
10.3 Function-Code 03: Reading up to 16 16bit-Registers .....15
10.4 Function-Code 06: Writing of 16 16bit-Registers.............15
10.5 Register Assignment .........................................................16
10.5.1 Definitions.....................................................................16
10.6 FLUO SENS integrated Memory Allocation......................17
10.7 Examples ........................................................................... 19
10.8 Reading “Version” ............................................................ 19
10.9 Reading “Temperature” .................................................... 19
10.10 Reading “Method Type” ................................................ 19
10.11 Reading measurement value “On Value 1”.................. 19
10.12 Setting “Start Method Command” ................................ 19
10.13 “Start Method Command” and Reading Data .............. 19
11 Controlling via Hyper Terminal .................................... 20
12 The FL Digital Program................................................. 20
13 Troubleshooting and Problem Solving ....................... 21
14 Maintenance and Care .................................................. 22
15 Warranty and Customer Services ................................ 22
15.1 Liability for Defects ........................................................... 23
15.2 Warranty............................................................................. 23

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1 General Product Description
Confocal fluorescence detector for the simultaneous
measurement of two separate fluorescent dyes.
Ultra-compact fluorescence detector
High sensitivity (e.g. Fluorescein down to 10-12 mol/l )
Confocal optics
Contact-free measurements on surfaces and in fluids
1 or 2 intensity controlled excitation light sources
1 or 2 highly sensitive, low-noise, long-term stable photodiodes
Broad spectrum of filters and excitation light sources for a
range of fluorescent dyes
Robust metal housing
Digital command interface
The unique fluorescence detector is the perfect solution for
implementation in compact and mobile fluorescence detectors or for
online measurements in automated processes.
The confocal optics allows contact-free measurements on surfaces
and in liquids in the presence of ambient light.
The highly sensitive detector measures fluorescent samples down to
very low concentrations and is easy to integrate as a component into
your instrument.
The compact and robust module contains a complete fluorescence
measurement device: precise micro-optics, powerful excitation light
sources, highly sensitive sensors and microprocessor controlled
electronics.
Calibration data and correction factors can be stored in the detector
and applied directly to the raw data.
Consequently the detector module provides direct results, not just raw
data. Measurement results can be accessed via a simple serial
interface. External devices such as PCs, embedded controllers or
PLCs can readily access measurement results via the integrated serial
interface.

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2 Technical Specifications
Performance
Absolute Detection Limit
e.g. <10-12 mol/l Fluorescein-
Sodium in 0.1N sodium hydroxide
Linearity
4 Decades
Noise level
<1 mV @ max. range
Short term stability
≤ 3% / h, for 1 second
measurement interval
Excitation
High performance-LED
Detection
Low-noise, precision Si-
Photodiode
Reference signal
measurement
Feedback loop with direct
measurement of excitation
intensity
Measurement intervals
0.1 seconds to hours
Measurement range
2 mm² focused and 25 mm²
unfocused
Distance
(Detector/Object)
2 mm focused to 25 mm
unfocused
Available excitation
wavelengths
265nm .. 980nm
Available detection
wavelengths
365nm .. 980nm
Specifications given in the list above are for reference only. Exact specifications for the
individual detectors are given in the corresponding data sheets.
Environmental Conditions
Temperature Range
+10°C to +40°C
Air humidity
20% - 70% rel. humidity,
without condensation
Air Pressure
300 - 1060hPa
Mechanics
Housing
Aluminum, anodized
Dimensions (without
adapter)
64x47x17.8mm³
Weight
90g
Protection Class
IP20
Electronics
Power Supply
+5VDC ±5%, ripple ≤20mV for
electronics
+5VDC ripple ≤20mV for LED
Power Input
Detector: 30mA
LED:≤150mA (depending on LED)
Interface
Serial, 57600 baud, 1 start byte, 8 data
bytes, no parity, 1 stop byte
Interface models
TTL- / RS485-level (0 / 3.3V)
RS232-level ±6V
Connectors
10 way flex cable
MOLEX 98267-0257
mating receptable
MOLEX 52610-1071

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3 Connections
Pinout of the connection cable
Pin
No.
Name
Description
1
Ground
Ground return for supply an communication signals
2
Ground
Ground return for supply an communication signals
3**)
TxD
Output of the serial interface
4*)
#Trigger
Low active input to trigger a measurement by hardware
5**)
RxD
Input of the serial interface
6
Ground
Ground return for supply an communication signals
7*)
#RESET
Low active input to reset the detector
8
+5V
Detector supply voltage
+5VDC, ±5%, acceptable ripple < 20mV
power consumption ≤40mA
9
Ground
Ground return for supply an communication signals
10
VLED
LED supply voltage +5VDC, acceptable ripple < 20mV
power consumption (depending on LED type) ≤150mA
The FLUO SENS integrated features a 10way flex cable in the rear
part of the housing (see 4.1 and 4.2). This flex cable is used for
communication and power supply. Dimensions are shown left.
Flex cable type: MOLEX 98267-0257
Matching connector (SMD) MOLEX 52610-1071 (not included)
Pin assignment of the flex cable is shown in the table left. Note the
following:
*): These inputs do not provide internal pull up resistors. In normal
operation they should be tied to +5V/+3.3V.
**): The levels of the serial interface are with respect to the detector
type either ±6V (RS232), 0/+3.3V (TTL).
In the TTL-version the TxD-signal (pin 3) is disconnected when not
used for data transmission. So, multiple devices can be connected in
parallel. All devices will receive data simultaneously, activate the TxD
pin and respond only if the modbus address matches.
All ground return pins should be connected to achieve optimal quality
measurement results.
16,5cm

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4 Physical Dimensions
4.1 ESMO40-MB-xxxx Detector
The ESMO40-MB-xxxx has its detection axis parallel to the top plate.
Physical dimensions are shown below.
The housing of the detector is connected to ground return.
To achieve precise alignment between the detector and the sample,
the front lens is designed with a Ø8f8 fit (shown bottom left).
ESMO40-MB-xxxx Detector Dimensions.
4.2 ESMO30-MB-xxxx Detector
The ESMO30-MB-xxxx has its detection axis perpendicular to the top
plate. Physical dimensions are shown below.
The housing of the detector is connected to ground return.
ESMO30-MB-xxxx Detector Dimensions.

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4.3 ESMO35-MB-xxxx Detector
The ESMO35-MB-xxxx has its detection axis in 70° to the top plate.
Physical dimensions are shown left.
The housing of the detector is connected to ground return.
ESMO35-MB-xxxx Detector Dimensions.
5 Operating Conditions
While using the , please note the following:
Surrounding Temperature: +10 °C to +40 °C
Transport Temperature: -45 °C to +65 °C
Relative Humidity: 20% to 70% rel. humidity
5.1 Environment
If you plan to use the device in a working environment prone to dust
and dirt, you will need to clean the device regularly. For cleaning, use a
damp cloth. For more persistent stains, it is also possible to clean the
surface with a cloth moistened with pure alcohol (isopropanol or
ethanol). Avoid the use of aggressive solvents such as acetone.
Do not expose the device to direct sunlight
Protect the device from high humidity and contact with liquids
Do not expose the device to excessive heat
Do not expose the device to strong electromagnetic radiation

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5.2 Safety Instructions
Tip!
While connecting an external trigger signal or external
power supply, the maximum permissible power should
not be exceeded (see section 2.2)
Tip!
The device may heat up in normal operating mode.
Tip!
Never touch the detector's optics.
,
Danger!
Do not look directly into the optics! Detectors emit
strong light radiation that can cause permanent
damage to the eye.
Danger!
Do not operate the device in environments where
explosions are possible.
Danger!
Do not use device on patients.
Warning!
Do not immerse the device in water.
Warning!
The device should only be connected and
disconnected in power-off mode.

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6 Contents of the FLUO SENS integrated Package
Carefully unpack the contents of the package and check that all
components (shown left) are contained therein. For claims, please
contact customer service.
The contains the following components:
The FLUO SENS integrated
6.1 Start up
The is ready for use upon delivery.
6.2 Mounting
The housing possesses two M3 threads on both long sides for
mounting purposes.
The positions of the threads are given in the drawings in chapter
4. Please note that the maximum permissible depth of the fixing
screws is 4mm. Longer screw threads can cause permanent
damage to the detector.
In addition the ESMO40 - series detectors can be clamped using
the Ø8mm optics nozzle.
Caution!
The maximum permissible depth of the thread is
4mm.
The FLUO SENS integrated

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7 Establishing Connection with a Computer
7.1 Connecting a Single Detector
In order to connect the detector, a 10way flex cable is attached
at the backside of the detector. The function is to transfer power
as well as communication
A more detailed description of the pin assignment can be found
in section 3.
7.2 Connecting Multiple Detectors
Up to 16 RS485 or TTL detectors can be attached parallel to a
master. The MODBUS specifications allow up to 255 devices,
however the built-in drivers limit the number to 64 for RS485
and 16 for TTL versions.
The MODBUS address for each device must be set at a value
between 1 and 254 before being connected.
Each attached detector can be simultaneously prompted to
carry out a fluorescence measurement by using the Start
command in tandem with the MODBUS address „255“ or the
external trigger input.
This method can be utilized for multiple measurements of
microtitre plates.
Tip!
When multiple detectors are interconnected, the
driver load and the pulse-like current intake of the
LEDs must be taken into account.
Tip!
The RS485 version can also be connected to a
conventional RS232 or USB interface via an
interface converter.

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8 Instructions for Usage in Compliance with EMC-
Directive 89/338 EWG
In use, the is not intended to be operated independently and is
exclusively manufactured for further use in EMC-competent
industries and companies
The detectors may only be used by trained personnel who are
qualified to carry out installation, operation and maintenance of
the devices in accordance with any relevant regulations.
The user must insure that the devices and the accompanying
components and equipment are mounted and connected in
compliance with local legal and technical regulations. In
Germany, this includes the VDE and Employer's Liability
Insurance Association regulations, as well as the stipulations of
the EMC directives
8.1 EMC-Safety Precautions
In the case of increased radiation rates in terms of the EMC law
(EMVG), the radiation can be suppressed by routing the
connecting cable through a suitable ferrite core, for example
Würth Elektronik 7427221.
Additionally, the FLUO SENS Integrated housing can be
earthed to ground using one of the mounting threads.
8.2 Switching on the Device
Immediately after plugging in the interface plug and the power
source, the is ready to use. No further procedure must be
carried out.
8.3 Switching off the Device
To switch off the device, simply remove it from the power
source. Any parameters which are stored in the device will not
be lost.

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9 Operation
is configured and operated exclusively via MODBUS-ASCII
commands. No further operation elements are necessary.
9.1 MODBUS-ASCII
The MODBUS Protocol was developed and released by the
company MODICON in the early 1980s. It is primarily employed
in the area of process automation. Because it is an open
protocol and, if nothing else, its simple structure, it is widely
used. In the meantime, the MODBUS-IDA Organization is
responsible for the maintenance and continuing development of
the protocol. Numerous documents can be obtained.
MODBUS is based on a master/slave system. The master is a
superordinate controller, for example a PC or a programmable
controller. The MODBUS Controllers in the FLUO SENS
integrated are slave devices.
There are no bus conflicts, since only a single node sends at a
time. The master requests communication. This request can be
directed to a specific node or sent out as a message to all
nodes. The nodes receive the request and send, independently
of the type of request, a reply to the master.
MODBUS communication is implemented as follows:
M
MODBUS serial communication via serial interfaces such
as RS232, RS485
M
MODBUS ASCII codes the data using ASC-II character
set in the form of legible character chains
MODBUS works according to the Master/Slave principle. A
master can communicate with one or more slaves. Only slaves
explicitly permitted by the Master can return data to the Master.
The protocol supports both binary and 16-bit values, which are
read in block-form by the Master. Neither quality recognition, nor
time stamps are supported.

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10 Configuring a MODBUS-Protocol
The MODBUS-ASCII Protocol is integrated into the . All protocols
are coded in hexadecimal values, represented by readable ASCII
characters. Only the characters 0-9 and A-F are used for coding
purposes. For each byte of information, two bytes are required
for the communication, due to the fact that in the hexadecimal
system, only 4 bytes of information can be transmitted.
Protocol Start: Character “:” (colon)
Coding: hexadecimal, ASCII characters 0-
9, A-F
Serial Interface: 1 start bit, 8 data bits, no parity,
1 stop bit
Check sum: Longitudinal Redundancy Check
(LRC)
Protocol End: CRLF
Baud rate: 57600 (standard value)
Start
Address
Function
Value
LRC
Check
End
1
character
2
characters
2
characters
N
characters
2
characters
2
characters
CR LF
Tip!
Detectors always respond to Write/Read Access
with MODBUS address 0. This is useful, for
example, if the address in register 165 has been
altered.
10.1 Longitudinal Redundancy Check (LRC)
The Longitudinal Redundancy Check (LRC) field is one byte,
containing an 8–bit binary value. The LRC value is calculated by
the transmitting device, which appends the LRC to the message.
The receiving device recalculates an LRC during receipt of the
message, and compares the calculated value to the actual value
it received in the LRC field. If the two values are not equal, an
error results. The LRC is calculated by adding together
successive 8–bit bytes in the message, discarding any carries,
and then two’s complementing the result. The LRC is an 8–bit
field, therefore each new addition of a character that would result
in a value higher than 255 decimal simply ‘rolls over’ the field’s
value through zero. Because there is no ninth bit, the carry is
discarded automatically. A procedure for generating an LRC is:
1. Add all bytes in the message, excluding the starting ‘colon’
and ending CRLF. Add them into an 8–bit field, so that
carries will be discarded.
2. Now built the twos–complement –the result is the LRC byte.
10.2 MODBUS Function Codes
The supports the MODBUS-ASCII Protocol with the function
codes 3 and 16. The data points are handled via register
addressing. A register consists of 16 bits. Measurement values
are transferred as long-values and consist of 2 registers. In this
manner, data points can be used directly without decimal points.
Function Code 03: Reads up to 16 16bit-registers
Function Code 06: Provides write access to 16bit-registers

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10.3 Function-Code 03: Reading up to 16 16bit-
Registers
This protocol reads up to 16 16bit-registers from a connected
device.
The following example reads 2 registers (32bit) from the
addresses 235 and 236.
The connected device was given the MODBUS address 9.
Request
Response
Field Name/Value (Hex)
Field Name/Value (Hex)
Start Frame
Start Frame
Slave Address 09
Slave 09
Function code 03
Function code 03
Start Register Hi 00
Number of Bytes 04
Start Register Lo EB
Value Hi (Register 235) 00
Number of Registers Hi 00
Value Lo (Register 235) 00
Number of Registers Lo 02
Value Hi (Register 236) 00
LRC 07
LRC F1
End Frame CRLF
End Frame CRLF
10.4 Function-Code 06: Writing of 16 16bit-Registers
This protocol writes a 16bit-register to a connected device.
The following example writes 1 register (16bit) on the address
235.
Request
Response
Field Name/Value (Hex)
Field Name/Value (Hex)
Start Frame
Start Frame
Slave Address 09
Slave Address 09
Function code 06
Function code 06
Start Register Hi 00
Register Address Hi 00
Start Register Lo EB
Register Address Lo EB
Value Hi (Register 235) 00
Value Hi 00
Value Lo (Register 235) 00
Value Lo 02
LRC 06
LRC 04
End Frame CRLF
End Frame CRLF

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10.5 Register Assignment
For communication purposes, the MODBUS-ASCII Protocol has
been installed in the . The following sections describe the storage
allocation.
10.5.1 Definitions
D1 = Measurement Values from Detection Channel 1
D2 = Measurement Values from Detection Channel 2
E1 = Excitation Channel 1
E2 = Excitation Channel 2
Cycles
Number of measurements to be carried out after the Start
command has been sent.
Cycle
Time
Interval in seconds between two measurements within a cycle.
Start
mode
By command: If a „1“ has been written in location 512,
the measurement will be started
Trigger single: Measurement is started by sending the
“Start Method Command”. One data sequence is taken
for each h/w trigger until the method is finished.
Trigger measurement: Measurement is started by
sending the “Start Method Command”, all values are
taken after one h/w trigger
Auto command: Measurement is started at power on,
the stored method will run once
Auto trigger single: Measurement is started at power
on, one value is taken for every h/w trigger until the
method is finished
Auto trigger measurement: Measurement is started at
power on, all values are taken after one h/w trigger
Method Type
E1D2: Emission on channel 1, detection on channel 2
S_E1D1: Scope mode E1D1. The detector records
1500 data points and saves them in registers 513-3513.
Dark Signal
Type
No dark signal (LED off):No dark signals recorded
Dark signal once: At the beginning of a measurement
cycle, one dark signal will be recorded.
Dark signal every: At the beginning of a measurement
cycle, dark signal will be recorded for every
measurement value.
Average
Number of measurement values used to calculate an
average.
LED Mode
Toggle: LED controlled by the detector
Manual: LED must be set manually before
measurement is starting
Trigger Delay
Wait time in ms before a measurement is started via
trigger impulse
On Delay LEDx
The detector waits for “On Delay LEDx” ms after the
LEDx has been turned on before a measurement is
carried out.
Off Delay LEDx
The detector waits for “On Delay LEDx” ms after the
LEDx has been turned off before a dark signal
measurement is carried out.
LEDx Power
Default
“LEDx ” standard LED set value for LED x
LEDx Power Max
Maximum allowed set value for “LEDx Power”
LEDx Power Min
Minimum allowed set value for “LEDx Power”

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10.6 FLUO SENS integrated Memory Allocation

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Special:
Modbus address 255 is the broadcast address, the device doesn't
send a response to this request.
Remark:
For byte parameters in bytes 0 or 2 (like Modbus Adress...) the given
values written to the detector must be multiplied by 256.
Example:
Set Modbus Address to 2 you have to write 2 * 256 = 512 to the
detector word address 164.
Registers 400 to 511 are general purpose registers for storage of any
user data (available in firmware version 1.46, dated 19.12.2011 and
higher).

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10.7 Examples
10.8 Reading “Version”
Request: ":0003018000106c"
Response:
":00032056312E303328363429204F6374203137
2032303037009000C6E0FD3395E0FC7408"
Interpretation of characters: V1.03(64) Oct 2007
10.9 Reading “Temperature”
Request: ":000301020002f8"
Response: ":00030400033ACBF1"
Integer Interpretation: 0x0033ACB = 211659 counts
Conversion counts - °C: ((counts*0,000298026) –54,3) / 0,205
211659 counts = 42,8°C
10.10 Reading “Method Type”
Request: ":000300030001f9"
Response: ":0003020100FA"
Interpretation of characters: 0x01 = 1 = E1D1
10.11 Reading measurement value “On Value 1”
Request: ":000301040002f6"
Response: ":000304001C5A364D"
Integer Interpretation: 001C5A36h = 1858102 counts
Conversion counts - mV:counts * 0.000298026
1858102 Counts = 553,76mV
10.12 Setting “Start Method Command”
Request: ":000602000001F7"
Response: ":000602000001F7"
10.13 “Start Method Command” and Reading Data
The detector starts a measurement when a „1“ is written in
register 512.
The data counter in the registers 256..257 (ticket) is increased
after each measurement value has been written in the
respective register (On Value 1...3, Off Value 1...3).
The measurement values must be read cyclically from the
respective registers if the ticket counter has been altered. With
cyclical readouts, the current measurement values will each be
overwritten by the latest valid measurement values.
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