RADLEY Mya 4 User manual

January 2020 |Issue 8
Mya 4 Reaction Station
Instructions

Mya 4 Reaction Station –Instructions
Issue 8 Page: 2
Contents
1 Introduction 5
2 Mya 4 Standard Warranty 6
2.1 Mya 4 Warranty Exclusions 7
3 Safety information 8
4 Important warnings 9
5 Component guide 10
6 Set-up and operation 11
6.1 Requirements 11
6.2 Getting started 11
6.3 Set-up overview 11
6.3.1 Connecting the Reaction Station to the mains power and the Control Pad 12
6.4 Coolant supply 13
6.4.1 Connecting the coolant supply to the Reaction Station 13
6.4.2 Optimising cooling performance 15
6.5 Internal gas purge 17
6.5.1 Connecting to the internal gas purge 17
6.6 Internal drain 19
6.6.1 Connecting to the internal drain 19
6.7 Reflux & Manifold Heads 20
6.7.1 Attaching the Reflux Head to the Reaction Station 22
6.7.2 Connecting the coolant supply to the Reflux Head 24
6.7.3 Connecting a single coolant supply to the Reaction Station and Reflux Head 26
6.7.4 Connecting the gas/vacuum supply to the Reflux Head 33
6.7.5 Important tips for optimum refluxing 36
6.7.6 Attaching the Manifold Head to the Reaction Station 37
6.7.7 Connecting the coolant supply to the Manifold Head 39
6.7.8 Connecting a single coolant supply to the Reaction Station and Manifold Head 41
6.7.9 Connecting the gas/vacuum supply to the Manifold Head 49

Mya 4 Reaction Station –Instructions
Issue 8 Page: 3
6.7.10 Attaching glass condensers to the Manifold Head 51
6.7.11 Important tips for optimum refluxing 53
6.8 Stirring options 54
6.8.1 Magnetic stirring 55
6.8.2 Overhead stirring (also called mechanical stirring) 55
6.9 Mya 4 reaction vessel options 56
6.10 Mya 4 inserts 57
6.11 Reflux tubes and multi-neck lids 59
6.11.1 Assembling glassware and a reflux tube 60
6.11.2 Attaching an Easy-On cap to a reflux tube 65
6.11.3 Assembling a reaction vessel and a multi-neck lid 66
6.12 Using overhead stirrers 68
6.12.1 Assembling the Mya Compact Stirrer 69
6.12.2 Stirrer guide care 72
6.13 Using external temperature probes 75
6.13.1 Connecting an external temperature probe 76
7 The Mya 4 Control Pad 79
7.1 Set-up and operation 80
7.2 Using the Control Pad 81
7.3 Overview screen 82
7.3.1 Apparatus 83
7.3.2 Profile 85
7.3.3 Graph 86
7.4 Zone View screens 87
7.4.1 Manual mode 88
7.4.2 Profile mode 91
7.4.3 Using graphs in Zone View 97
7.4.4 Zone settings 100
7.5 File Management 104
7.5.1 Individual zone file management 104
7.5.2 General file management (all zones) 109
7.6 System Settings 122
7.6.1 System settings tab 124

Mya 4 Reaction Station –Instructions
Issue 8 Page: 4
7.6.2 Service Settings tab 126
7.6.3 PID Gains tab 128
7.6.4 Network Share tab 129
8 Safety 130
8.1 Chemical resistance 130
8.2 Electrical safety 131
8.3 Temperature safety 131
8.3.1 Printed Circuit Board (PCB) sensors 131
8.3.2 Block temperature (Tb) sensors 131
8.3.3 Coolant temperature (Tc) sensors 132
8.3.4 Reaction temperature (Tr) sensors 133
8.3.5 Max temperature difference (Tb–Tr)max 134
9 Temperature control 135
9.1 Minimising temperature overshoots 135
9.1.1 Pre-set Temperature Control options (PID settings) 135
9.1.2 Creating a ramped profile step 143
9.1.3 Adjusting the Max Temperature Difference: (Tb–Tr)max 146
9.2 Configuring your own PID values 148
10 Error handling 151
10.1 Yellow Zone error 151
10.2 Red Zone error 153
10.3 Red System error 155
10.4 Error codes 156
11 Technical specifications 158
12 Component compatibility guide 162

Mya 4 Reaction Station –Instructions
Issue 8 Page: 5
1Introduction
Thank you for purchasing the Mya 4 Reaction Station.
Please read this instruction manual thoroughly before operating your unit.
Mya 4 Reaction Station
•Four independent zones
•Magnetic and overhead stirring
•-30 °C to +180 °C
•2 ml to 400 ml
•Software control
www.radleys.com/products/our-products/mya-4-reaction-station
Radleys accepts no liability if users do not observe the instructions for the safe operation of the
Mya 4 Reaction Station as set out in this instruction manual.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 6
2Mya 4 Standard Warranty
The Mya 4 Standard Warranty is included free of charge as part of your Mya 4 Reaction Station
purchase. It ensures that you have full remote and on-site support for the first 12 months of your
Mya 4 experience.
The Warranty period commences from the delivery date of the Mya 4 Reaction Station.
The Warranty covers all Mya 4 manufactured components, defined as the following:
•Mya 4 Reaction Station
•Mya 4 Control Pad
•Mya 4 Manifold Head
•Mya 4 Reflux Head
•Mya Compact Stirrer
•Mya Inserts
•Data Hub
The Warranty includes:
•priority email and telephone support,
•remote set-up and application support,
•the repair and/or the replacement of any necessary parts,
•on-site repairs anywhere in the world (if deemed necessary by Radleys),
•all travel, accommodation, subsistence and labour costs relating to an on-site visit,
•swap out or loan unit (if deemed necessary by Radleys),
•all shipping charges for loan, swap out, return to base units and parts,
•replacement unit for ‘out-of-box’ failures (if deemed necessary by Radleys),
Note An ‘out-of-box’ failure is defined as a failure of any of the Mya 4 manufactured components
(see above) immediately after the product purchase, and when the failure occurs when installing
or performing initial configuration of the item. The period to make an ‘out-of-box’ failure claim is
limited to one month from date of delivery.
Any claims under this Warranty must be made before the end of the warranty period in writing to
Radleys, Shire Hill, Saffron Walden, Essex, CB11 3AZ. United Kingdom.
All Radleys manufactured components shall be fit and sufficient for the purposes as defined by
Radleys, and shall be covered for any defects in material and/or performance.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 7
2.1 Mya 4 Warranty Exclusions
The following are excluded from the Mya 4 Standard Warranty:
•Repairs and parts outside the 12 month warranty period (including the cost of labour, travel,
accommodation, subsistence and shipping):
→It is the customer’s responsibility to pay for repairs and parts outside the warranty
period. All replacement parts, labour, travel, accommodation, subsistence and shipping
will be charged at current list prices.
→Purchase orders will be required for all non-warranty work prior to
commencement of the work.
•Normal maintenance, damage due to fire, flood or other acts of nature, customer misuse,
unsuitable utilities, electrical disturbances, improper installation, improper or unauthorised
repairs, or damage incurred during shipping or transit.
•All third-party products. This includes, though is not limited to:
→recirculator/chiller
→pumps
→balances
→pH meters & probes
→PC or laptop, etc.
→Ethernet switch
Note Radleys will honour the manufacturing warranties of all ‘third-party’ equipment that it
supplies, i.e. equipment not manufactured by Radleys. Radleys will also pursue warranty claims
on the customer’s behalf for any equipment we undertake to repair. Please note that ‘third-
party’ manufacturers will require proof of purchase and date of purchase before they will process
any warranty claim.
•All glassware, consumables and accessories. This includes, though is not limited to:
→glass flasks, vessels, tubes, vials, lids, condensers, funnels, adapters, stoppers, etc.
→PTFE or glass stirrer shafts/bars
→PTFE caps
→tubing
•Any financial claims resulting from the unavailability of parts.
•Compensation for labour other than that of Radleys personnel.
•Altering, removing or tampering with the serial number voids all warranties.
•Unauthorised opening or modification of the product, including usage for other than the
intended purpose, voids all warranties.
•Radleys reserves the right to make any necessary charges for the late cancellation of an arranged
on-site visit, where the cancellation results in additional costs.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 8
3Safety information
The following comments are included to assist the user in the safe and efficient operation of the
Mya 4 Reaction Station.
Warning
Applies when there is a possibility of personal injury or damage to equipment.
Notes
→Provides the user with additional information.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 9
4Important warnings
Mya 4 should only be operated by trained and competent personnel.
As with all chemistries, a full risk assessment should be performed prior to using the Mya 4 Reaction
Station, and care should be taken to monitor reactions at all stages. If the Mya 4 unit is left
unattended whilst in operation, it is the user’s responsibility to ensure that local rules for
unattended working are complied with.
Please read the following warnings before using Mya 4, and ensure you fully understand them.
The user must:
•only operate Mya 4 in a fume hood with protective safety sash,
•ensure that Mya 4 is positioned on a level surface, and that all vents and fans are unobstructed,
•always read the instructions for any third-party equipment (e.g. recirculator/chiller, pumps) to
ensure no safety recommendations or manufacturing warranties, when used in conjunction with
Mya 4, are contravened,
•ensure Mya 4 is correctly set up, and be responsible for the continued maintenance of the Mya 4
Reaction Station, and all of its accessories,
•ensure the working temperature range of the Mya 4 Reaction Station is -30 °C to +180 °C,
Note Mya 4 can be used at ambient temperatures ranging from 10 to 30 °C.
•take care not to touch the aluminium inserts or reaction vessels during and after heating/cooling,
as there is the risk of burns from hot or extreme cold surfaces (Mya 4 inserts can remain hot for a
considerable time after the heating source has been switched off),
Note When the Mya 4 Reaction Station and Control Pad are both on, a heatwave or snowflake
warning icon will be displayed on the Control Pad to indicate when a zone is at 40 °C or warmer,
or 0 °C or colder.
•check that all fluid hoses and connections are in good condition, fully secure and not leaking,
before starting any experiments,
•always wipe away any chemical spills immediately, and avoid exposure to strong vapours such as
HCl, etc. Mya 4 external surfaces are resistant to the majority of solvents and splash-resistant to
dilute acids and alkalis at room temperature, however, continued exposure to chemical vapours
or liquids will cause corrosion to metal and/or plastic components.
Note Mya 4 can be used with a circulating cooling fluid at a temperature from 5 °C to 35 °C
without damage to the unit. Some condensation may be observed when working at the lower
temperatures.
•regularly perform careful inspection of all fluid connections to Mya 4.
•ensure all wires and cables connecting auxiliary equipment are in good condition, and secured so
that they cannot come into contact with Mya 4 surfaces or moving parts.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 10
5Component guide
Front view
Rear view

Mya 4 Reaction Station –Instructions
Issue 8 Page: 11
6Set-up and operation
6.1 Requirements
You will need:
•a mains power socket,
•a flow of coolant:
-mains water (supply + drain) or a recirculator/chiller with water/ethylene glycol mix,
-from 5 °C to 15 °C (up to 35 °C, however performance will be affected),
•an inert gas supply (x2 or divided at the source),
•a drain connection (or a container at floor level) to evacuate any water collected inside the
Reaction Station due to condensation.
6.2 Getting started
Remove the Mya 4 Reaction Station from its packaging and position in a fume hood, ensuring that:
•the front vent and rear fan exhaust of the Reaction Station are unobstructed,
•there is adequate space for the Mya Control Pad to be positioned inside the fume hood or
nearby,
•the unit is level and stable,
•the unit is close to suitable services (see Requirements above).
6.3 Set-up overview
1Connect the Reaction Station to the mains power and to the Control Pad. See page 12.
2Connect the coolant supply to the Reaction Station. See page 13.
Note If you will be using a Reflux/Manifold Head and a single source of coolant, please skip
this step.
3Connect to the Internal Gas Purge. See page 17.
4Connect to the Internal Drain. See page 19.
5If you are attaching a Reflux or Manifold Head, see page 20. Otherwise, skip to point 7.
6 If you are using a Reflux/Manifold Head: connect the coolant supply and inert gas to the
Reflux Head (see page 24) or the Manifold Head (see page 39).
7Choose between magnetic or overhead stirring. See page 54.
8 Choose and set up your glassware and inserts. See page 56.
9 Turn on the coolant supply/supplies.
10 Turn on the gas source(s).
11 Turn Mya 4 on using the on/off switch on the side of the instrument.
12 Wait for Mya 4 Control Pad to boot up, and display front screen. See Section 7 on page 79.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 12
Notes
→When no experiments are running, it is recommended to switch off the coolant supply.
6.3.1 Connecting the Reaction Station to the mains power
and the Control Pad
Step 1
Plug one end of the mains power
cable into the mains inlet socket at
the rear of the unit.
Plug the other end into the mains
supply.
Step 2
Plug one end of the Mya 4 Control
Pad cable into the controller socket
at the rear of the unit.
Ensure the cable is fully secured
using the securing screws.
Step 3
Plug the other end of the cable into
the socket at the rear of the Mya
Control Pad.
Again, ensure the cable is fully
secured using the securing screws.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 13
6.4 Coolant supply
Mya 4 requires a liquid coolant supply to be connected and running at all times when the unit is in
operation. The coolant flows through Mya 4’s internal heat exchangers, which are located in each
reaction zone, to dissipate heat from the cooling Peltier cells.
Coolant operating limits
Coolant supply Mains water or ethylene glycol mix (max 30% vol.)
Max coolant temperature 35 °C
Min coolant temperature 5 °C
Max flow rate 5 L/min
Min flow rate 2 L/min
Max coolant pressure 2 bar
Min coolant pressure 0.3 bar
Coolant in/out connectors Quick release, barbed 9.5 mm for 8–10 mm ID tubing
If using a thermoregulator:
Both specs below required:
Min cooling power at 15 °C 1.5 kW
Min cooling power at 0 °C 1.0 kW
Always use the maximum flow available from the thermoregulator, up to a maximum of 5 L/min
6.4.1 Connecting the coolant supply to the Reaction Station
If you are using a Reflux or Manifold Head, and using a single coolant supply, see page 20 for
information on how to connect the coolant supply to the Reflux/Manifold Head and the Reaction
Station.
To connect the coolant supply to the Reaction Station, you will need these components (supplied
with Mya 4):
Coolant flowmeter
RR40633
Coolant tubing
RR40627
Tubing clamps
RR40634
Quick-release male
connector
RR95525

Mya 4 Reaction Station –Instructions
Issue 8 Page: 14
Notes
→To ensure correct operation of Mya 4, it is important to control and monitor the coolant flow within the stated
operating limits at all times by using the coolant flowmeter (RR40633) supplied.
→All tubing should be fully secured using a tubing clamp (RR40634). Slide the tubing clamp over the end of the tubing
before connecting the tubing to either a quick-release connector or other component, such as a flowmeter.
Warning
Do not use a high-pressure water/coolant source above 2 bar as there is the risk of internal leaks, tubing splitting or
connectors failing.
Step 1
Remove the orange blanking plugs
(RR40828) from the Coolant In and
Out female connectors at the back of
the Reaction Station.
Note Keep the blanking plugs safe as
you may need to reinsert them if
moving Mya 4 to a different location.
(See Notes below.)
Step 2
Connect a length of coolant tubing
from the coolant supply (tap water or
recirculator) to the lower fitting of the
coolant flowmeter (RR40633).
Secure with a tubing clamp (RR40634).
Note We recommend reinforced
coolant tubing RR40627.
Step 3
Connect another length of coolant
tubing to the upper fitting of the
coolant flowmeter, securing with a
tubing clamp.
Now attach the quick-release male
connector (RR95525) to the other end
of this tubing, again securing with a
tubing clamp.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 15
Notes
→The condition of tubing and connectors should be regularly checked, in line with good laboratory practice.
→If a quick-release male connector does not fit into a female connector, check that the metal locking mechanism is in
the open position.
→Most of the quick-release connectors on the Reaction Station and its accessories feature a non-return shut-off valve
to prevent coolant flowing when the unit is disconnected. However, the female connectors at the rear of the
Reaction Station do not have shut-off valves. If you need to move Mya 4 to a different location, use the blanking
plugs (see Step 1 above) to block these connectors off during transportation.
6.4.2 Optimising cooling performance
When in operation, the temperature and flow rate of the coolant will have an effect on the
performance of Mya 4, particularly when performing reactions below ambient temperatures.
•With coolant at 5 °C, block temperatures (Tb) down to -30 °C can be achieved.
•With coolant at 15 °C, block temperatures (Tb) down to -25 °C can be achieved.
•When a zone is being actively cooled, the temperature of the coolant held within Mya 4 will rise.
This coolant temperature is monitored for each zone, and if this temperature rises above 35 °C,
a warning is displayed and the experiment will stop. (See Error Codes on page 156.)
Step 4
Insert the end of the tubing + male
connector into the Coolant In inlet at
the rear of Mya 4.
Step 5
Take another length of coolant tubing
and attach a quick-release male
connector to one end, securing with a
tubing clamp. Insert this end into the
Coolant Out outlet at the rear of Mya
4. Connect the other end to a suitable
drain if using tap water, or to the
return connector if using a recirculator.
To drain or
recirculator

Mya 4 Reaction Station –Instructions
Issue 8 Page: 16
Notes
→Coolant must be flowing at all times when running an experiment, even when reactions are not being cooled.
→If the coolant supply is not switched on before an experiment is started, or the flow rate is too low, an error will be
detected, a warning displayed and heating/cooling will stop. (See Error Codes on page 156.)
→If the coolant temperature is below the minimum temperature (5 °C) an error will be detected, a warning displayed
and heating/cooling stopped. Coolant temperature needs to be reset by the user to a value above the minimum.
→Coolant should be kept running after an experiment or series of experiments have been stopped, until block
temperatures have returned to close to ambient temperature. If not, heat (or cold) from the block may transfer to the
static coolant in the heat exchanger, causing it to rise above the maximum (or fall below the minimum) coolant
temperature, creating an error message.
→Once the block temperatures have returned to close to ambient temperature, it is recommended to switch off the
coolant supply when the Reaction Station is not in use.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 17
6.5 Internal gas purge
A flow of inert or dry gas (nitrogen, argon, dry compressed air) is essential to protect the internal
components of Mya 4 from ambient hazardous or corrosive gases and vapours, as well as
condensation of atmospheric moisture.
Purge gas
Max flow rate 15 L/min
Min flow rate 3 L/min
6.5.1 Connecting to the internal gas purge
To connect to the internal gas purge, you will need these components (supplied with Mya 4):
Gas flowmeter
RR40632
Gas & water tubing
RR40626
Tubing clamps
RR40634
Notes
→To ensure correct operation of Mya 4, it is important to control and monitor the purge gas flow within the stated
operating limits at all times by using the gas flowmeter (RR40632) supplied.
→All tubing should be fully secured using a tubing clamp (RR40634). Slide the tubing clamp over the end of the tubing
before connecting the tubing to a connector.

Mya 4 Reaction Station –Instructions
Issue 8 Page: 18
Step 1
Connect a length of gas tubing from
the inert/dry gas supply to the lower
fitting of the gas flowmeter (RR40632),
securing with a tubing clamp
(RR40634).
Note We recommend gas & water
tubing RR40626.
Step 2
Connect another length of gas tubing
to the upper fitting of the gas
flowmeter, again securing with a
tubing clamp.
Step 3
Now slide the other end of this gas
tubing onto the Internal Inert Gas
Purge In connector at the rear of
Mya 4, securing with a tubing clamp.
Notes
→Mya 4 is not hermetically sealed, therefore the inert/dry gas will escape from the unit into the atmosphere.
Warning
Operating Mya 4 without an inert/dry gas purge can damage the instrument and will void the warranty.
From inert/dry
gas supply

Mya 4 Reaction Station –Instructions
Issue 8 Page: 19
6.6 Internal drain
In the unlikely event of an internal leak from the coolant circuit, fluid will be confined and
channelled to the Internal Drain Out outlet at the rear of Mya 4. To remove this fluid, you will need
to connect the Internal Drain Out outlet to a suitable drain.
6.6.1 Connecting to the internal drain
To connect to the internal drain, you will need these components (supplied with Mya 4):
Gas & water tubing
RR40626
Tubing clamps
RR40634
Slide a length of tubing onto the Internal
Drain Out connector at the rear of Mya 4,
securing with a tubing clamp (RR40634).
Now place the other end of this tubing in a
suitable drain (or a container that is
regularly emptied, if no drain is available).
Notes
→When operating at low temperatures or in high-humidity conditions, some internal condensation may form within
the unit. This condensation is normal and will be confined and channelled to the Internal Drain Out outlet, but some
residue may remain within the unit.
→If you need to move Mya 4, it is recommended that you first slightly tilt the unit from left to right, and front to back,
to ensure that all residual fluid is removed.
Warning
The tubing leading from the Internal Drain Out to the drain must be level with or lower than the Internal Drain Out
connector to ensure liquid properly drains from inside the unit, preventing serious damage.
To drain

Mya 4 Reaction Station –Instructions
Issue 8 Page: 20
6.7 Reflux & Manifold Heads
Unless you are only using vials and/or
tubes in your Mya 4 (see example picture
on right) a removable head is required to:
•allow the glassware to stand securely in
the Reaction Station,
•distribute inert gas to each position,
•minimise solvent evaporation and allow
for refluxing.
Two head options are available for Mya 4: a Reflux Head or a Manifold Head.
Reflux Head
Manifold Head
Reflux Head
Provides fully integrated reflux operation without the need for additional condensers when used
with reaction vessels and reflux tubes. It also provides a convenient integrated inert gas supply to
each reaction vessel through the Easy-On cap, Easy-On manifold cap or Compact Stirrer Easy-On
head. (See page 33 for more information on Easy-On caps.)
Manifold Head
Provides a convenient integrated water supply for use with condensers in each zone and an inert gas
supply to each reaction vessel through the Easy-On cap, Easy-On manifold cap or Compact Stirrer
Easy-On head. (See page 49 for more information on Easy-On caps.)
The Manifold Head is supplied with 4 x tubing and connectors (RR40816) to connect the Manifold
Head to a Mya coil condenser (RR40556) in each of the four positions. (See page 51 for information
on how to do this.)