RAL instruments STAINER User manual

The RAL Automated staining system
User Guide
RAL STAINER
The RAL Automated staining s
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Use
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Guide
Ref:12060-V05 / EN

RAL STAINER
2 RAL STAINER User guide
Before use, please read the user guide carefully in order to be able to use the equipment correctly.
The user guide must be kept in the immediate vicinity in order to be available for reference at all
times.
All rights reserved. The reproduction, modification or use of any part of this document in any form is prohibited
without the written permission of RAL Instruments.
RAL DIAGNOSTICS - Site Montesquieu - 33650 Martillac - FRANCE
E-mail: [email protected]
Tel: [phone] - Fax: [phone]
www.ral-instruments.com
History of changes to the user guide
Revision Date Section Software Version
1 03/2012 2.43
2 07/2012 6 2.55
3 08/2012 12 2.55
4 02/2013 - 2.60
5 07/2013 - 2.63
Updates available on www.ral-instruments.com.

RAL STAINER
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1 INTRODUCTION.....................................5
1.1 RESPONSIBILITY...................................5
2 SYMBOLS.........................................6
2.1 SYMBOLS USED IN THIS GUIDE ........................6
2.2 DESCRIPTION OF THE SYMBOLS ON THE APPLIANCE .........6
3 WARRANTY AND WARRANTY LIMITS .....................7
4 SAFETY INSTRUCTIONS...............................8
4.1 COMPLIANT USE...................................8
4.2 WARNINGS.......................................8
4.3 WASTE..........................................9
4.3.1 ELECTRONIC WASTE ..............................9
4.3.2 BACTERIOLOGICAL AND CHEMICAL WASTE...............9
5 DESCRIPTION OF THE APPLIANCE ......................10
5.1 OPERATING PRINCIPLES ............................13
5.2 PERFORMANCE...................................13
5.3 SAFETY ........................................13
5.4 SIZE ..........................................13
5.5 ELECTRIC SPECIFICATIONS ..........................13
5.6 ENVIRONMENTAL LIMITS FOR USE .....................14
5.7 ENVIRONMENTAL LIMITS FOR TRANSPORTATION AND STORAGE 14
6 STAINING REAGENTS ...............................15
7 INSTALLATION .................................... 16
7.1 PACKAGING LIST..................................16
7.2 INSTALLATION ENVIRONMENT ........................17
7.3 SETTING UP .....................................17
7.4 CONNECTION OF THE INSTRUMENT TO THE MAINS POWER SUPPLY
7.5 HYDRAULIC CONNECTIONS ..........................18
8 SCREEN DESCRIPTIONS ............................. 19
8.1 MEANINGS OF THE SYMBOLS ........................19
8.2 PAGE DESCRIPTIONS ..............................20
8.2.1 STAINING PAGE .................................20
8.2.2 SETTINGS PAGE.................................21
8.2.3 CHANGE SESSION PAGE...........................23
8.2.4 INFORMATION PAGE ..............................24
8.2.5 ALPHANUMERIC AND NUMBER KEYBOARD..............25
9 INITIALISATION .................................... 26
9.1 USER CREATION AND RIGHTS MANAGEMENT .............26
10USE...........................................29
10.1 STARTING UP ...................................29
10.2 INSTALLING REAGENTS ............................29
10.2.1 STAINING KIT..................................29
10.2.2 RINSING SOLUTION ............................. 30
CONTENTS

RAL STAINER
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10.3 STAINING PROTOCOL ............................. 30
10.3.1 PROTOCOL MODIFICATION ........................31
10.3.2 CHANGING THE PROTOCOL........................ 33
10.4 INSTALLING THE SLIDES ...........................33
10.4.1 LOADING THE SLIDE HOLDER ......................33
10.4.2 PLACE THE SLIDE HOLDER IN THE APPLIANCE ..........33
10.5 STAINING ......................................34
10.6 CHANGING THE STAINING KIT OR DRAINING .............34
10.6.1 CHANGING THE STAINING KIT ......................34
10.6.2 DRAINING ....................................35
10.7 STAND-BY .....................................35
10.8 POWERING OFF..................................35
11 TRACEABILITY ................................... 36
11.2 LOADING THE TRACEABILITY FILE .....................36
12 CLEANING ......................................37
12.1 CLEANING .....................................37
12.2 HYDRAULIC SYSTEM CLEANING ......................37
12.3 ACTIVATED CARBON FILTER .........................38
12.4 DECONTAMINATION............................... 39
13 CONSUMABLES AND OPTION EQUIPMENT ...............40
13.1 CONSUMABLES .................................40
13.2 OPTIONAL EQUIPMENT ............................40
14 MAINTENANCE ...................................41
15 AFTER-SALES SERVICES ............................41
16 TROUBLESHOOTING ...............................42
16.1 ERROR MESSAGES ...............................42
16.2 WHAT TO DO WHEN ...?............................43
17 SUMMARY OF TIPS................................ 45
18 INDUSTRIAL PROPERTY ............................46
19 EC DECLARATION .................................47
20 INDEX .........................................48

RAL STAINER
5 RAL STAINER User guide
Thank you for having chosen RAL Stainer.
RAL Stainer is intended for the staining of haematological or bacteriological smears.
It can be used to autonomously stain 20 slides and returns the slides ready for observation.
It recognises the staining kits installed and recommends the protocol to use as well as the renewal of the
reagents.
It can trace staining carried out using an RFID system.
It ensures the complete safety of the user: the user loads and unloads the biological samples without
opening the appliance, the residual stain emanations are retained by an activated carbon filter.
1.1 RESPONSIBILITY
Even if the greatest care has been given to the drafting of this guide, RAL Instruments cannot be held liable
by any party in the event of damages caused, or supposed to have been caused, either directly or indirectly, by
the failure to carry out the instructions contained in this guide or by the non compliant use of the equipment
described below.
1 INTRODUCTION

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2 SYMBOLS
2.1 SYMBOLS USED IN THIS GUIDE
RAL Stainer is designed in order to ensure the maximum safety of the user. However, it is necessary to follow
the instructions described in this guide and indicated by the following symbols:
Attention : Failure to respect the information preceded by this symbol can be dangerous for the
user or for the environment ; or cause major damage to the appliance.
Warning: This symbol precedes all information covering the correct use of the appliance and a
quality diagnosis.
Biological risks
Information: This symbol precedes information that may be useful to a user operating the appliance.
Tips: This symbol precedes useful advice.
2.2 DESCRIPTION OF THE SYMBOLS ON THE APPLIANCE
Biological Risks
REF Appliance Reference
SN Appliance Serial Number
Manufacturer's Address
Fabrication Date
Operating relative humidity limits
Operating temperature limits
Recycling of the instrument or return to the distributor
Pollution Control
Attention, see User Guide
Consult the User Guide
Medical In Vitro Diagnosis System
Security Certification
European Compliance
Back sideFront side

RAL STAINER
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3 WARRANTY AND WARRANTY LIMITS
The duration of the warranty for equipment and manufacturing faults is indicated on your warranty contract.
The warranty period starts on the day your RAL Instruments representative commissions the equipment.
Failure to respect the safety instructions below or a use which is not compliant with this user guide may use
cause the cancelling of the warranty.
The warranty does not cover wear parts.

RAL STAINER
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4.1 COMPLIANT USE
r3"-4UBJOFSJTintended for the staining of haematological or bacteriological smears. It operates using RAL
Stainer kits from RAL Diagnostics. It is not designed for any other use and cannot be operated using other
reagents than those from RAL Diagnostics.
r5IFTNFBSTXJMMCFNBEFPOTUBOEBSETMJEFT
r3"-4UBJOFSJTTUSJDUMZSFTFSWFEGPSJOUFSJPSVTF
r5IFVTFPGUIFBQQMJBODFNVTUDPNQMZXJUIUIFJOTUSVDUJPOTHJWFOJOUIJTHVJEF
r5IFDPOEJUJPOTPGDPNQMJBOUVTFBMTPJODMVEFUIFSFHVMBSDMFBOJOHPGUIFBQQMJBODFBOEUIFDBSSZJOHPVUPG
maintenance operations.
r5IFVOQBDLJOHJOTUBMMBUJPOBOESFQBJSPQFSBUJPOTNVTUCFDBSSJFEPVUCZBOBVUIPSJTFEUFDIOJDJBO
rThe reservoir tank must be always in place. See section 7.3.
r5IJTEFWJDFDPNQMJFTXJUI1BSUPGUIF'$$3VMFT0QFSBUJPOJTTVCKFDUUPUIFGPMMPXJOHUXPDPOEJUJPOT
this device may not cause harmful interference, and (2) this device must accept any interference received,
including interference that may cause undesired operation.
4.2 WARNINGS
r Failure to respect the safety instructions and the use instructions may compromise the user safety .
r/FWFS DPOOFDU UIF BQQMJBODF UP B NBJOT QPXFS PVUMFU TVQQMZJOH B WPMUBHF EJGGFSFOU UIBO UIBU
indicated on the rear of the appliance. The main supply must not exceed ± 10% of the specified
voltage (100-240V). Failure to follow this instruction can cause a fire or an electric shock.
r"WPJEDBVTJOHEBNBHFUPUIFQPXFSTVQQMZDBCMF%POPUQMBDFBOZBQQMJBODFTPOJU%POPUUVH
on the cable (to disconnect the appliance for example). Failure to follow these instructions can
cause a short circuit or a disconnection that could, in turn, cause a fire or an electric shock.
r
Before connecting the product to the mains, check that the electrical installation of the building
complies with national installation regulations.
r Do not use a power cable of a length greater than 3m .
r/FWFSUPVDIUIFFMFDUSJDDJSDVJUTPSPUIFSQBSUTMPDBUFEJOTJEFUIFBQQMJBODF5IFSFJTBSJTLPG
electric shock, especially with damp hands.
r5IFMJUIJVNCBUUFSZDPOUBJOFEJOUIFFMFDUSPOJDDBSEMPDBUFEJOUIFMFGUSFBHFOUESBXFSPGUIFEFWJDF
can be replaced by a battery with the same characteristics (CR1220 3v). If not, it may cause an
explosion. Throwing away used batteries according to your laboratory practises.
r*OUIFFWFOUPGVOVTVBMTNPLFCFJOHFNJUUFECZUIFBQQMJBODFJNNFEJBUFMZTXJUDIJUPGGVTJOHUIF
main switch and unplug the mains power supply cable. Contact the RAL Instruments post-sales
service.
r*UJTQSPIJCJUFEUPGPSDFUIFDPWFSPQFOJOHTUIFTMJEFESBXFSTPSUIFSFBHFOUESBXFSTCPUIXIFO
operating and when not operational.
r*UJTQSPIJCJUFEUPGPSDFUIFSPUBUJOHBSNCPUIXIFOPQFSBUJOHBOEXIFOOPUPQFSBUJPOBM
r8FBS MBCPSBUPSZ HMPWFTBOE BMBCPSBUPSZDPBU XIFO VTJOHUIF BQQMJBODF BOEJUTSFBHFOUT 5IF
dangers relative to each reagent are indicated on their respective packaging or see section 6. In
the event of an accident, refer to the relevant safety data sheets.
rAll biological samples, all effluents and all used consumables should be considered to be
potentially dangerous. Once the appliance is no longer used, the user should disinfect and
wash his/her hands.
rThe contact between contaminated hands and the appliance can cause the contamination of
the appliance. In this case, decontaminate the appliance according to current laboratory best
practises. See also section 12.2.
r(FOFSBMMZNBLFTVSFUIBUDVSSFOUMBCPSBUPSZCFTUQSBDUJTFTBSF used.
The guarantee is not responsible for any changes or modifications not expressly approved by the
party responsible for compliance. Such modifications could avoid the user's authority to operate
the equipment.
4 SAFETY INSTRUCTIONS

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4.3 WASTE
4.3.1 ELECTRONIC WASTE
This symbol is applicable in European countries.
It indicates, in compliance with the 2003/108/EC directive relating to electric and electronic waste,
that electric and electronic objects are subject to selective waste collection by the company that
brought them onto the territory. Contact your RAL Instruments representative.
Outside the European Union, refer to current local or national regulations.
4.3.2 BACTERIOLOGICAL AND CHEMICAL WASTE
Before disposing of biological samples and/or chemical reagents, consult the reagent safety instructions.
Generally, make sure that current laboratory best practises are used.

RAL STAINER
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5 DESCRIPTION OF THE APPLIANCE
Open front face view
Left view Right view
Side view with
drawers open
Top view with
drawers open