REPLIGEN XCell Lab XC-LAB-S User manual

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XCell™ Lab Controller
User Guide
•
PLC Software Version 1.00
•HMI Software Version 1.11
•Surface Pro SP7+
•Windows Version 10 Pro 20H2
•Devices supported XCell ATF® 1, 2 and 4

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The information contained in this document is subject to change without notice.
With respect to documentation accompanying Product, Repligen makes no warranty, express or
implied. Any and all warranties related to the documentation accompanying Product are expressly
disclaimed. Customer shall refer to the terms and conditions of sale governing the transaction for
any and all warranties for the Product.
Repligen Corporation shall not be liable for errors contained herein or for incidental or
consequential damages in connection with the furnishing, performance, or use of this material.
No part of this document may be photocopied, reproduced, or translated to another language
without the prior written consent of Repligen Corporation.
Products are not intended for diagnostic or therapeutic use or in vivo with humans or animals.
For further information, please contact Repligen Corporation at www.repligen.com.
©2022 Repligen Corporation. All rights reserved. The trademarks mentioned herein are the property
of Repligen Corporation and/or its affiliate(s) or their respective owners.
Customer Support
[e-mail]
[phone] (Option 1)
Repligen Corporation
41 Seyon Street
Building 1 Suite 100
Waltham, Massachusetts 02453
www.repligen.com

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Contents
6.5.1 A2B and A2C tubing sets.............................................................................................18
6.9.1 Windows environment ...............................................................................................20
6.9.2 Repligen XCell™ Lab Software ....................................................................................20
6.9.3 Modbus/ethernet remote monitoring .......................................................................20
7.6.1 Menu bar ....................................................................................................................24
7.8.1 Changing ATF Flow set points .....................................................................................28
7.8.2 Starting, pausing and stopping an XCell ATF® Device.................................................28
7.8.3 Pump status box .........................................................................................................28
7.8.4 Dual mode...................................................................................................................29
7.8.5 ATF displacement volume pop-up window ................................................................32
7.8.6 ATF pressure pop-up window.....................................................................................32
7.8.7 Settings sub-menu ......................................................................................................33
7.8.8 XCell ATF® Device configuration.................................................................................33
7.8.9 Bioreactor configuration.............................................................................................34
7.8.10 Default configuration/ATF pump settings ..................................................................34
7.8.11 General configuration.................................................................................................35
7.8.12 Service /performance screen......................................................................................36
7.8.13 Logon required - security on/off.................................................................................36
7.8.14 Algorithm pop-ups ......................................................................................................37
7.9.1 XCell™ Lab Trend screen.............................................................................................38
7.11.1 Alarm Configuration....................................................................................................40

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7.11.2 ATF Flow alarm configuration.....................................................................................40
7.11.3 Displacement volume alarm configuration ................................................................41
7.11.4 System alarm configuration........................................................................................41
7.11.5 Permeate (P3) pressure alarm configuration .............................................................42
7.12.1 Historian Client Software............................................................................................43
7.12.2 Trend...........................................................................................................................43
7.12.3 Query Tool ..................................................................................................................44
7.12.4 Exporting data to Excel ...............................................................................................44
14.2.1 Changing usernames and passwords..........................................................................61

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List of tables
Table 1. Explanation of user attention phrases................................................................................10
Table 2. Safety precautions examples..............................................................................................10
Table 3. Safety warnings...................................................................................................................11
Table 4. XCell™ Lab Controller model comparison ..........................................................................16
Table 5. Controller ports...................................................................................................................18
Table 6. A2B Tubing ID/OD...............................................................................................................19
Table 7. SAPA components...............................................................................................................19
Table 8. Preset usernames and passwords ......................................................................................22
Table 9. Starting, pausing and stopping an XCell ATF® Device instructions.....................................28
Table 10. Pump status messages examples .......................................................................................29
Table 11. ATF modes - independent and dual ...................................................................................30
Table 12. Starting, pausing and stopping XCell ATF® Device(s) in Dual Mode...................................31
Table 13. ATF service parameters ......................................................................................................36
Table 14. Alarm status........................................................................................................................40
Table 15. Recommended ATF rate to Filtration rate ratios ...............................................................48
Table 16. XCell™ Lab System Price and Parts List - for Sales..............................................................51
Table 17. Windows usernames and passwords .................................................................................60
Table 18. XCell™ Lab users and roles .................................................................................................60
List of figures
Figure 1. XCell™ Lab Controller and accessories ................................................................................9
Figure 2. ATF pressure and exhaust strokes.....................................................................................14
Figure 3. Backflush example.............................................................................................................14
Figure 4. Configuration - bioreactor, controller, and utilities ..........................................................15
Figure 5. Face A of Controller...........................................................................................................17
Figure 6. Face B of Controller ...........................................................................................................17
Figure 7. SAPA components example...............................................................................................19
Figure 8. Touchscreen splash display example ................................................................................21
Figure 9. Login screen example ........................................................................................................22
Figure 10. Overview/SCADA screen example.....................................................................................22
Figure 11. Menu bar example ............................................................................................................24
Figure 12. User information ...............................................................................................................24
Figure 13. ATF main screen example two XCell ATF® Devices attached to same vessel ...................25
Figure 14. Example - 2 synchronized XCell ATF® Devices attached to the same bioreactor .............26
Figure 15. Example - 2 XCell ATF® Devices attached to two bioreactors...........................................26
Figure 16. Example - Dual controller and single XCell ATF® Device ...................................................27
Figure 17. ATF Flow pop-up example.................................................................................................27
Figure 18. XCell ATF® Device status - running, paused or stopped....................................................28
Figure 19. ATF DUAL Pop-up example................................................................................................30
Figure 20. ATF displacement volume pop-up example......................................................................32
Figure 21. ATF pressure pop-up screen example...............................................................................32
Figure 22. Settings sub-menu example ..............................................................................................33
Figure 23. XCell ATF® Device configuration example.........................................................................33
Figure 24. Bioreactor configuration example.....................................................................................34
Figure 25. ATF pump settings example ..............................................................................................34
Figure 26. General configuration example.........................................................................................35
Figure 27. Service/performance example..........................................................................................36
Figure 28. ATF-A Algorithm pop-up example.....................................................................................37
Figure 29. ATF-A and ATF-B algorithm pop-ups .................................................................................37
Figure 30. XCell™ Lab Trend screen example.....................................................................................38
Figure 31. Alarm condition example ..................................................................................................39

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Figure 32. Alarm status ......................................................................................................................39
Figure 33. Alarm Configuration example ...........................................................................................40
Figure 34. Displacement volume alarm example...............................................................................41
Figure 35. System alarms example.....................................................................................................41
Figure 36. P3 permeate pressure alarm example ..............................................................................42
Figure 37. Accessing Trend and Query example ................................................................................43
Figure 38. Trend screen example.......................................................................................................43
Figure 39. Query example ..................................................................................................................44
Figure 40. Columns pane - subset of the Query window...................................................................45
Figure 41. Query set-up......................................................................................................................45
Figure 42. Help feature.......................................................................................................................46
Figure 43. Workflow for a typical ATF start-up process.....................................................................47
Figure 44. Control Panel network and sharing center .......................................................................56
Figure 45. Control Panel’s network adapter properties ....................................................................56
Figure 46. Windows 10 network adapter TCP/IPv4 properties .........................................................57
Figure 47. Default values for ATF Pump Configuration......................................................................66
Figure 48. Default values for ATF Flow Alarms ..................................................................................66
Figure 49. Default values for Displacement Volume alarms..............................................................67
Figure 50. Default values for System Alarms .....................................................................................67
Figure 51. Default values for P3 Permeate Pressure Alarms .............................................................68

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Abbreviations
A-B Allen-Bradley
AC Alternating current
Amp Ampere
ATF Alternating tangential flow
ATF-A XCell ATF® Device A
ATF-B XCell ATF® Device B
AUX Auxiliary
A2B XCell ATF® Device to Bioreactor connection
A2C XCell ATF® Device to Controller connection
CFM Cubic feet per meter
CSPR Cell specific perfusion rate
dB Decibels
DC Direct current
DO Dissolved oxygen
DPv1 PROFIBUS Decentralized Peripherals version 1
FAS Field Applications Scientist
FC Flow control
FS Flow sensor
FSE Field Service Engineer
HFM Hollow Fiber Module
HMI Human Machine Interface
Hz Hertz
ID Inner diameter
I/O Input/output
kg Kilograms
L Liter
lbs. Pounds
LPM Liters per minute
mA Milliamp
mL Milliliter
mV Millivolt
NPT National pipe thread
OD Outer diameter
OSI Open systems interconnection
PCV Pressure control valve
PID Proportional, integral and derivative
PLC Programmable logic controller
POI Product of interest
PRV Pressure regulating valve
PV Process value
P2 PCV outlet pressure (Commanded pressure)
P3 Permeate pressure
PPE Personal protective equipment
psi Pounds per square inch
psig Pounds per square inch gauge
QC Quick Connect
SAPA Supply Air Protection Assembly
SCADA Supervisory Control and Data Acquisition
SP Set point
SUB Single-use Bioreactor
TC Tri-clamp
TCD Total cell density

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TCP/IP Transmission control protocol / Internet protocol
UF Ultrafiltration
VCD Viable cell density
VDC Volt direct current
VT Volumetric throughput
VVD Vessel volume exchange per day

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1. Introduction
The XCell ATF® Technology provides a complete solution for the retention of cells, removal of media
and, ultimately, the intensification of upstream cell culture processes. It is the most widely used and
proven cell retention solution in the world, having been specified in dozens of commercial
processes. Thousands of XCell ATF® systems are currently utilized in process development and
clinical manufacturing processes. As the gold standard for upstream process intensification, XCell
ATF® often delivers at least 10 times higher cell concentrations or product throughput than typical
batch and fed-batch processes.
The XCell ATF® System includes several components: a controller (with air and vacuum accessories),
software, sensors and XCell ATF® Device.
This User Guide serves as a reference document for your XCell™ Lab Controller and software;
updates are made on a regular basis. For the latest version of this document, please visit the
Repligen website. Installation by a trained Repligen Field Service Engineer (FSE) is highly
recommended.
2. Welcome
Thank you for choosing the XCell™Lab Controller for your laboratory and cell culture intensification
needs. This next generation system provides proven upstream intensification performance on an
industrial automation platform. The XCell™ Lab Controller combines performance intensification and
ease of use into a single solution. Key components include an easy-to-use software, flow sensors,
and air and vacuum accessories (Figure 1). The XCell™ Lab Controller is designed to meet the
challenges of the next wave of high-density intensification processes, which are expected to exceed
250 x 106 cells/mL.
Figure 1. XCell™ Lab Controller and accessories
1. XCell™ Lab Controller
2. XCell™ Lab Software and HMI
3. Flow sensor
4. Supply air protection assembly
5. Vacuum manifold
6. Vacuum pump
Existing XCell ATF® users may jump to Section 5for an abbreviated setup description.
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3. About this document
This manual uses several user attention phrases. Each phrase should draw the following level of
attention:
Table 1. Explanation of user attention phrases
Phrase Description
Note: Points out useful information.
IMPORTANT Indicates information necessary for proper instrument operation.
PRECAUTION
Cautions users of potential physical injury or instrument damage if the information is not
heeded.
WARNING! Warns users of potential serious physical injury if warnings are not heeded.
4. Safety precautions
Table 2. Safety precautions examples
Description of precautions
Use of safety glasses is recommended during installation, set up, operation and while any service
or testing is being performed on the system.
You must use the Supply Air Protection Assembly (SAPA) on your air source to ensure air is
brought to a safe pressure, and suitably filtered to ensure pump functionality.
Vessels or bioreactors should not be pressurized unless otherwise specified by the manufacturer.
Glass and single-use bags can explode if pressurized. When using any vessel or bioreactor, be
sure to maintain an unrestricted vent or exhaust from the vessel. This will ensure that no
significant pressure or vacuum occurs in the bioreactor. In the case of a diaphragm failure, for
example, air will penetrate the XCell ATF® Device, through the filter and into the vessel. A free
exhaust from the vessel will minimize the build-up of pressure in the vessel.
You must replace the air filter on Stainless Steel XCell ATF® Devices (ATF2 and ATF4) prior to
operation.
When not in use, you should ensure all the tubing is capped or blocked using the provided caps
and valves. It is recommended to keep unused tubing in the original sealed bag.

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Table 3. Safety warnings
Description of warnings
Power: Do not open powered-on XCell™ Lab Controller enclosure. Remove power from
equipment before attempting any maintenance. Calibration, preventive maintenance and repair
to be performed only by trained, qualified personnel.
Use only Repligen provided power supply.
Use only high voltage cord specific for your region provided by Repligen.
Do not use a damaged power supply or damaged power cord.
Tubing: Tubing breakage between the XCell ATF® filtration device and bioreactor may result in
fluid being sprayed from pump. Use appropriate measures to protect operator and equipment.
Do not perform cleaning (wipe down) of the XCell™ Lab Controller when the enclosure cover is
open. Confirm all connectors (connection bulkheads) are firmly seated in their respective sockets.
Weight: XCell™ Lab Controller weighs 44 lbs. (15.5 kg). When the enclosure must be moved, use
necessary precautions prior to and during the movement. Make sure no electrical, pneumatic or
signal connections are made when system is moved.
Wear standard laboratory PPE, including lab coat, protective eye wear and gloves.
Air and vacuum: XCell™ Lab Controller requires pneumatic connections of positive air pressure
and vacuum using Repligen provided hoses with safety quick connectors. Positive air pressure
tubing is colored red while vacuum tubing is colored blue. The connection points are identified as
Pressure and Vacuum.
The pneumatic lines should be kept free from dust and particles. Please ensure the following:
•The air filters on the controller in the A2C line are always present, and changed out during
the annual PM.
•The A2C lines have ball valves that should be turned to closed position when they are not
connected to an XCell ATF® Device.
•When the equipment is not being used, the A2C lines should be capped.
•The SAPA unit should always be connected to the controller.
Supply Air Protection Assembly (SAPA): While almost all labs do filter incoming air, Repligen
cannot warranty the controller without the use of a SAPA on the incoming line, which filters the
air entering the XCell™ Lab controller. The Supply Air Protection Assembly must be mounted to a
solid support, either a wall or table.

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5. XCell™ Lab Controller quick set-up for experienced users
If you are an experienced XCell ATF® user and already familiar with the Set-up Guide and Health and
Safety guidelines, then this section will help you get set-up quickly. If you need further guidance,
please review the full User Guide, including the Appendices, or reach out to a local FAS.
5.1 Connections
Connect all components as described below:
a. Orient the controller on the bench as you see appropriate for your bioreactor setup. A
maximum of two controllers can be stacked vertically.
b. Place the tablet in a convenient location, either on the controller or attached to a shelf.
c. Connect the air utility line to the SAPA, through the manifold and then to up to four
controllers. Use the supplied adaptors if needed.
d. Connect your lab vacuum supply or Repligen supplied vacuum pump to the vacuum manifold.
Use the supplied adaptors if needed.
e. Prepare the XCell ATF® Device(s) according to the appropriate Device Set-up Guide.
f. Connect the XCell ATF® Device(s) to the bioreactor using the A2B tubing kit supplied by
Repligen.
g. Use the correctly sized/labelled A2C tubing for the XCell ATF® Device you plan to use.
h. Make sure the flow sensor is correctly oriented and stably positioned on the A2B tubing in a
location where air bubbles are not likely to collect within the tubing.
i. Connect the permeate pressure sensor (P3), if used.
j. To supply power to the controller, connect the 24 V adaptor to the wall power source.
k. Turn on the controller and tablet. By default, no logon credentials are required for the tablet.
The XCell™ Lab Software opens with the Supervisor account and displays the Overview screen
5.2 Software set-up
Please note the following:
•The newly designed software interface allows you to make real-time changes to the set points.
•During operation, certain buttons are pre-programmed to be greyed out and remain non-
functional to prevent unnecessary and undesired changes (for example, change of XCell ATF®
Device size is not allowed while the device is running).
•Certain options and buttons will not be visible if you are logged in as a User (limited access)
With auto login enabled, the default user level Supervisor allows complete access.
•Default settings typically are suitable for most processes; no further adjustments should be
required.
To get started:
a. On the main menu (top), click the Settings button, which displays the Settings sub-menu.
b. The first (and default) button, ATF configuration, allows selection of ATF size and filter type.
c. Click the second button, Bioreactor configuration, to change the default settings of one ATF
attached to a bioreactor.
d. Other buttons allow you to change date and time formats, pump settings, flow rates etc.
5.3 XCell ATF® Device start-up
a. Click the ATF button on the main menu to open the ATF Main Screen.
b. Click the ATF Flow box or the ATF graphic, both of which open a pop-up window.
c. Click Start. The priming sequence begins and the run starts.
Note: You can change the set points at any time before starting a run and during a run.

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5.4 Optimization
It is important to optimize process conditions. The guidelines in this document provide a useful
resource as you plan your process development, but please reach out to your local FAS for
consultation during optimization, scale-up and scale down experimental design or data review.
6. Overview of the XCell ATF® Technology and process intensification
The XCell ATF® Technology provides a complete solution for the retention of cells, removal of media
and ultimately the intensification of upstream cell culture processes. XCell ATF® Devices are often
used in continuous upstream processes, also called perfusion, but are equally beneficial in fed-batch
and hybrid processes. Examples of application of a cell retention device for fed- batch include:
•N-1 processes using short periods of perfusion to boost cell growth or regular media exchange
•High Productivity Harvest (HPH) application for fed-batch gene therapy, vaccine, mAb and
recombinant processes to boost productivity while also eliminating both centrifugation and
depth filtration unit operations.
•Perfusion for continuous processing
•Vaccine and viral process intensification
Repligen has an experienced global team of scientists ready to support the development,
optimization, scale-up and troubleshooting of XCell ATF® intensified cell culture processes. For
support or troubleshooting, please contact your local FAS.
A preventative maintenance (PM) contract ensures continued operation of the system at optimal
performance levels. The pneumatic parts that are connected to the controller and SAPA should
always remain free of dust and particles. Refer to Section 4for additional safety precautions.
6.1 Alternating Tangential Flow (ATF) Filtration
The diaphragm pump of the XCell ATF® System generates alternating tangential flow (ATF) through
hollow fiber filters. ATF is a low shear, rapid, pulsating and bi-directional flow of cell suspension
between a bioreactor and a diaphragm pump (Figure 2). Cell culture moves in a continuous back and
forth motion through the lumen of the hollow fiber filters. Two strokes of the diaphragm pump, the
Pressure stroke (P-stroke) and the Exhaust stroke (E-stroke), complete each back and forth cycle.
Delivery of positive air pressure to the base of the diaphragm by the pressure control valve in the
controller initiates the P-stroke. Positive air pressure pushes the diaphragm up from the air-side
hemisphere of the device, driving liquid from the diaphragm pump through the lumen of the hollow
fiber filters and back to the bioreactor. Replacement of the positive pressure under the diaphragm
pump with a vacuum initiates the E-stroke. The vacuum pulls the diaphragm down from the liquid-
side hemisphere of the device, pulling liquid from the bioreactor through the hollow fiber lumens
and towards the diaphragm pump.

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Figure 2. ATF pressure and exhaust strokes
6.2 ATF backflush
In most tangential flow filtration processes, liquid typically only moves across the filter from the
retentate side to the filtrate side. During ATF, liquid moves both from the retentate side to the
filtrate side as well as from the filtrate side to the retentate side. Liquid transfer from the filtrate
side to the retentate side is referred to as backflush and the action plays an essential role in the
differentiated ATF performance (Figure 3). Alternating flow generates negative pressure across the
hollow fiber filter during each pump stroke. The section of the hollow fiber experiencing the
negative pressure (and the consequential backflush) depends on the direction of the pump stroke.
Backflush effectively reduces or eliminates filter fouling by displacing material from the lumen wall
Incorrect ATF operation with insufficient pump displacement and/or low ATF flow rates create
reduced levels of backflush, which can compromise results. Smart mechanisms, built into the
algorithm and alarms (both default and user defined), automatically compensate and help minimize
fouling. The Troubleshooting Section, provides additional approaches and solutions to fouling and
other frequently observed challenges.
6.3 ATF flow rates and displacement volumes
Figure 3. Backflush example
Backflush in pressure stroke (left); backflush in exhaust stroke (right)

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The flow rate of cell culture liquid from A2B does not remain constant over the course of a pump
stroke. As the diaphragm begins to move from a stationary position, the flow rate starts relatively
low. As the diaphragm travels further, the flow begins to increase and reaches a maximum value
approximately at the midpoint. Toward the end of the diaphragm trajectory, the flow rate once
again begins to slow. The change in the flow rate over the course of the pump stroke may therefore
be considered to be sinusoidal. The controller records the instantaneous flow rate approximately
every ~100 ms during each stroke (ml/min) and then averages all stroke measurements over the
cycle to generate the ATF flow rate. The ATF flow rate contains data averaged over 10 cycles, which
largely eliminates outliers and noise.
You control the ATF flow rate by entering a value for the ATF Flow Rate Set Point. Due to the
sinusoidal nature of the flow rate over the course of an ATF cycle, the measured instantaneous flow
rate may be either higher or lower than the reported ATF flow rate over the course of each stroke.
Generally, you do not need to consider the Instantaneous Flow Rate, except during troubleshooting.
A well-controlled XCell ATF® Device will have the following flow rate properties:
•achieves maximum flow quickly
•switches smoothly between strokes
•minimal to no delay between strokes (referred to as the Dwell Time)
•maximum liquid displacement during strokes (referred to as Displacement Volume)
The recommended ATF Flow Rate Set Points and the typical Displacement Volumes are programmed
into the ATF System as default values. The calculated average displacement volume from the flow
sensor is updated every 30 minutes to further improve the response accuracy.
6.4 System configurations
Figure 4. Configuration - bioreactor, controller, and utilities
The XCell ATF® Lab System is designed to support multiple controllers simultaneously. Each
controller, in turn, supports multiple devices and bioreactors (Figure 4). The multiplicity from your
utilities to your bioreactors provides significant facility and configuration flexibility.
Both positive air pressure and the vacuum source lines connect to intermediate manifolds with one
input and four outputs. Volumetric air flow and vacuum requirements will vary with the number of
controllers as well as the number and the model of filtration devices used.

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A dedicated tablet running the XCell™ Lab software operates each controller
•Each controller supports up to 2 XCell ATF® Devices, depending on the model purchased
•The XCell™ Lab Controller will run XCell ATF® 1, 2 and 4 Devices, with adjustment of A2C
tubing, A2B tubing and flow sensor
•Two XCell ATF® Devices from a single controller may be connected to two bioreactors.
Alternatively, two XCell ATF® Devices from a single controller may be connected to a single
bioreactor for an increased rate of media exchange.
6.5 XCell™ Lab Controller
XCell™ Lab Controller is available in 3 different models (Table 4). Controller model number XC-LAB-
D-P utilizes a permeate pressure sensor to gain insight into real-time filter performance and fouling
characteristics. This data helps determine when a filter should be replaced and improves scale-up
prediction.
Table 4. XCell™ Lab Controller model comparison
Features XC-LAB-S XC-LAB-D XC-LAB-D-P
Single ATF Operation 
Dual ATF Operation × 
In/Out of Phase or Independent Dual Operation N/A 
Transmembrane Pressure (P3) × × 
Single Use XCell ATF® 1 
Single Use XCell ATF® 2 
Autoclavable XCell ATF® 2 
Autoclavable XCell ATF® 4 
If monitoring filter fouling and permeate pressure is important to your process, then you should
consider purchasing the D-P model as this feature is not available on the S and D models. If you
purchase an S model, you will not be able to upgrade your controller to a D model.The controller is
powered by a 110-220 V AC source that is converted to 24 V DC. International AC connectors for the
United States, United Kingdom, European Union and China are included with your shipment.
Locations that utilize an adaptor outside this set of four will require a customer provided adaptor.
The controller has two faces with utility ports and controls. Face A of the controller governs utility
inputs, including the vacuum, air and electric power to the enclosure (Figure 5). Face A also includes
the power switch for the controller. Note that some ports on Face A may not be used in the current
model.
Note: The utility tubing set provided connects to the air and vacuum ports (Figure 5). The air tubing
connects the controller to the SAPA, not directly to your lab utility source.
Face B of the controller (Figure 6) governs output operations, including connection to the XCell ATF®
Device and relevant sensors.

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Figure 5. Face A of Controller
Figure 6. Face B of Controller
1
2
3 4 5
7
8 9
6

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Table 5. Controller ports
Item Description
1 Air Positive air pressure from SAPA
2 Vacuum Vacuum line from facility line or vacuum pump manifold
3 Multi-pumps NOT CURRENTLY USED
4 Permeate A pump NOT CURRENTLY USED
5 Permeate B pump NOT CURRENTLY USED
6 AUX Pumps NOT CURRENTLY USED
7 Power switch Power on/off
8 24V DC in DC power from power supply
9 24V DC out NOT CURRENTLY USED
10 ATF A / B System pause
Pause buttons for ATF A and ATF B
LED light to indicate status
11 Alarm Visual alarm (audio optional)
12 To ATF A / B
A2C connections for air pressure and vacuum to ATF
filtration devices
13 Bioreactor A / B Weight NOT CURRENTLY USED
14 ATF A / B Flow Sensor
A2B retentate line flow sensor connections from filtration
devices
15 Ethernet
1: Communication between controller and tablet (M12 via
ethernet to RJ45 USB adapter on tablet)
2: NOT CURRENTLY USED
16 ATF A / B Permeate pressure Permeate line P3 pressure sensor inlet connections
17 VCD – Bioreactor A / B NOT CURRENTLY USED
Two manual pause buttons (item 10) allow each device to be independently paused. The alarm (11)
alerts the user when attention is required. The A2C lines (12) provide positive and negative air
pressure to the XCell ATF® Device. Pressure (16) and flow (14) sensor ports carry data from the
device-mounted sensor to the controller. An ethernet port (15) transfers data to a tablet for
viewing, processing, and analysis. Like Face A, some ports of Face B are not used in the current
model.
The software interface is accessed through a Surface Pro tablet, provided in a protective case. The
tablet may be mounted directly on the controller or to a shelf, using a bracket and moveable support
arm. Each controller requires a dedicated tablet that uses a wired ethernet connection to the
controller port.
6.5.1 A2B and A2C tubing sets
Each XCell ATF® Device requires a specific A2B and A2C tubing set size. The controller ships with a
collection of A2C tubing sets suitable for all devices (ATF1, ATF2 and ATF4). The A2C tubing may
appear to be similar – but they are not – and each is designed to function with a specific XCell ATF®
device size. Labeling on the tube sets indicates use with an ATF1, ATF2 or ATF4 device. The A2C
tubing set connects the XCell ATF® Device to the controller.
A flow sensor on the A2B line, engineered specifically for XCell ATF® applications, requires a specific
tubing OD and tubing type. Tube sets with a permeate pressure sensor also are available. Both the
flow sensor and the pressure sensor connect directly to the controller. The device specific setup-
guides describe tube set specifications in detail; a brief summary is provided in the table below.

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Table 6. A2B Tubing ID/OD
Device Tubing ID /OD Repligen Supplied Tube set?
XCell ATF® 1 1/8” / 1/4" Yes, part of device
XCell ATF® 2 1/4” / 3/8” Yes, supplied separately
XCell ATF® 4 3/8” / 5/8” Yes, supplied separately
Please reach out to your local Repligen representative for more information.
6.6 Supply Air Protection Assembly (SAPA)
Figure 7. SAPA components example
Table 7. SAPA components
Item Description
1 Manifold inlet Pressurized air from air supply
2 Filter unit
Filters air routing from air supply to manifold
Filter porosity: 0.3
3 Pressure regulator
Factory set air pressure supply regulator designed to
downregulate the supply air to a lower pressure required to
operate XCell ATF® 1, XCell ATF® 2 and XCell ATF® 4 (do not
adjust)
4 Pressure relief valve
Factory set air supply pressure relief valve set to relieve if
inlet pressure exceeds the specification noted above (do not
adjust)
5 Positive pressure supply manifold Pressurized air to XCell™ Lab Controller(s)
6 Manifold outlets Distributes air pressure to up to four XCell™ Lab Controllers
The SAPA normalizes air pressure from the lab utility line down to the required 15.6 psi. The
minimum utility air pressure requirement is 30 psi. A pressure relief valve provides safety in case of
the unlikely failure of the regulator (Figure 7). The regulator and relief valve are pre-set at the
factory; no modification is required by the end user. Installation should be performed or supervised
by an authorized Repligen service engineer.
Note: The SAPA must be installed with the air filter oriented downwards and plumbed.

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6.7 XCell ATF® Device preparation and set-up
Set-up guides, included with each XCell ATF® Device, describe how to assemble, wet, autoclave (if
stainless steel), test and connect the XCell ATF® Device to the controller. For stainless steel devices,
the autoclavable filter preparation guide comes in the box with each filter.
All Device Set-up Guides are available online.
6.8 System set-up
Please refer to the separate XCell™ Lab Controller Set-up Guide provided with the equipment.
Access the most up to date version online.
6.9 IT, Wi-Fi and network connectivity
The XCell™ Lab is designed as a stand-alone control system. Both a wired ethernet and Wi-Fi are
available for data transfer on the Surface Pro tablet. Repligen does not support integration of the
tablet into a network in any way. Please see Appendix B for further details.
6.9.1 Windows environment
The Surface Pro comes with Windows® 10 Pro installed. The user bears full responsibility for
modifications to the software system. These software changes include, but are not limited to,
Windows software updates, anti-virus applications, and Microsoft® Office products. While we do not
expect any impact to the functionality of the Repligen supplied programs through typical use and/or
Windows® maintenance, we cannot guarantee the correct functioning of the system.
6.9.2 Repligen XCell™ Lab Software
Repligen built XCell™ Lab Software is easy and intuitive to use. It is a Wonderware View application,
with several useful software modules included in this release including Historian, Query and Trend,
as described later in this guide.
6.9.3 Modbus/ethernet remote monitoring
Process data is recorded locally. The system currently does not support third-party interfaces or
remote monitoring.