Roth ROTIPHORESE PROfessional runVIEW User manual

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Horizontal Electrophoresis System
4849.1 ROTIPHORESE®PROfessional runVIEW System
4850.1 ROTIPHORESE®PROfessional runVIEW Base for
electrophoresis unit ROTIPHORESE®PROfessional III (2850)
9958.1 ROTIPHORESE®PROfessional runVIEW Base MINI 1 for
electrophoresis unit ROTIPHORESE®PROfessional I (2788)
9961.1 ROTIPHORESE®PROfessional runVIEW Base MINI 2 for
electrophoresis unit ROTIPHORESE® PROfessional II (2799)
runVIEW –made by Cleaver Scientific Ltd, UK
Warning: The runVIEW real-time horizontal DNA electrophoresis system has been thoroughly tested
and found to comply within the limits of CE regulation. It has been manufactured using the latest
technology and does not require maintenance. When used correctly this unit poses no particular
health risk, although it can deliver dangerous voltage levels if used incorrectly. Accordingly, this
power supply must only be operated by fully qualified personnel adhering to the guidelines laid
out within this instruction manual. Although this power supply is equipped with all necessary safety
features against abuse and accidental failure, caution should always be exercised when working with
high voltage equipment. Any individual intending to use this instrument should read the entire manual
thoroughly before operation.
The horizontal electrophoresis units from ROTH are designed for long term laboratory use and to
obtain reproducible results. Please spend a few moments reading the instruction manual thoroughly.
These units comply with the statutory CE safety rules:
73/23/EEC: Low voltage directive: IEC 1010-1:1990 plus amendment 1:1992
EN 61010-1:1993/BS EN 61010-1:1993
Please verify that you received the unit completely and without any damage. Any faults or losses have
to be reported to ROTH immediately. ROTH can not accept responsibility for goods that were sent
back without informing them.
Please take a look at the packing list and check whether all components and accessories are present.
Please retain all packaging material until the warranty period has expired.
Environmental Conditions
This unit may only be installed and operated only under the following environmental conditions:
oThis apparatus is intended for indoor use only.
oThe unit can be operated safely at an altitude of 2000 m.
oThe normal operating temperature range is ambient to 40 °C.
oAtmospheric pressure: 75 kPa –106 kPa
oRelative humidity: ≤95 %
This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.
POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution occurs. Occasionally,
however, a temporary conductivity caused by condensation must be expected”.
All ROTH products are supplied having passed rigorous quality control procedures. For
additional questions please give us a call: ++49-721-5606-0

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PACKING LIST
4849.1 ROTIPHORESE®PROfessional runVIEW System:
o1x runVIEW base station (including power cord)
o1x special lid with spectral emission filter and extractor fan within its viewing pane
for dyes with green and red fluorescence
o1x ROTIPHORESE® PROfessional III tank w/o lid, with electrodes, 1x 15 x 15 cm gel tray, 2x
casting dams
o1 pair of 4 mm power output cables
o8x double-sided combs:
2x 4-sample/16-sample (1 mm); 4x 20-sample/28-sample (1 mm, multichannel compatible);
1x 4-sample/16-sample (3 mm);1x 20-sample/28-sample (3 mm, multichannel compatible)
oInstruction manual
4850.1 ROTIPHORESE®PROfessional runVIEW
Base:
o1x runVIEW base station (including power cord)
o1x special lid with spectral emission filter and
extractor fan within its viewing pane for dyes with
green and red fluorescence
oInstruction manual
The lid is suitable to electrophoresis unit
ROTIPHORESE®PROfessional III (Art. No. 4850.1)
9958.1
ROTIPHORESE®PROfessional runVIEW Base MINI 1:
o1x blue light illuminator (adjustable to 2 different sizes, including
power cord)
o1x special lid with spectral emission filter and extractor fan within
its viewing pane for dyes with green and red fluorescence
oInstruction manual
The lid is suitable to electrophoresis unit ROTIPHORESE®
PROfessional I (Art. No. 2788.1)
9961.1
ROTIPHORESE®PROfessional runVIEW Base MINI 2:
o1x blue light illuminator (adjustable to 2 different sizes, including
power cord)
o1x special lid with spectral emission filter and extractor fan within its
viewing pane for dyes with green and red fluorescence
oInstruction manual
The lid is suitable to electrophoresis unit ROTIPHORESE®
PROfessional II (Art. No. 2799.1)
runVIEW base station
for PROfessional III unit
runVIEW base MINI 1 with special lid
for PROfessional I unit
runVIEW base MINI 2 with special lid
for PROfessional II unit

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METHOD
ROTIPHORESE®PROfessional runVIEW for real-time electrophoresis under blue light
ROTIPHORESE® PROfessional runVIEW is an innovative new system designed for real-time size
fractionation and recovery of nucleic acids. You can watch your bands migrate through the gel in blue
light –without mutagen or retina damaging effects.
The runVIEW system maximises the efficiency of DNA recovery from stained gels by minimising the
number of steps involved in post-electrophoretic purification. First choice for rapid gel analysis, gel
elution and all control gels.
TECHNICAL FEATURES
Real-time electrophoresis for ROTIPHORESE® PROfessional III unit
4849.1 ROTIPHORESE®PROfessional runVIEW System
4850.1 ROTIPHORESE®PROfessional runVIEW Base
The runVIEW system consists of a base unit with integrated power
supply and blue LED gel illuminator and a ROTIPHORESE®
PROfessional III unit with a special lid,
containing a spectral emission filter and extractor fan within its
viewing pane.
The lid is suitable for dyes with green fluorescence, e.g. the non-toxic dye SYBR® Green, and red
fluorescence, e.g. ethidium bromide
or the non-toxic dye ROTI®Gelstain Red (Art. No. 0984).
The blue light illuminator provides instant time-saving
visualisation of DNA/RNA band migration. No UV safety equipment required for blue light illumination.
For the ROTIPHORESE® PROfessional III unit, gel trays of 15 x 7, 15 x 10 and 15 x 15 cm are
available. The gel tank or the UV tray may be placed on the viewing platform for immediate
observation of the nucleic acid bands within the gel.
ROTIPHORESE® PROfessional runVIEW is suitable for ROTI®Load DNA Stain, ethidium bromide, and
SYBR®Green stained gels in real-time observation, post-run visualisation, or even post-run staining.
Operational (runVIEW Base)
Wave length (excitation)
470 nm (blue)
Voltage (V)
25 –150 (resolution 1 V)
Max. intensity of current (mA)
300 mA (resolution 1 mA)
Max. output
30 W
Timer
1 –999 mins. (alarm)
Display
LED display
Output type
Constant V or mA
Outlets (parallel socket pairs)
1 (4 mm)
Safety
”no load“ detection
Ambient temperature
15-40 °C
Dimensions (cm)
22 x 29.3 x 8 cm
Weight
2.6 kg
Current
100 –240 V
runVIEW base station with
PROfessional III unit and special lid

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Real-time electrophoresis for ROTIPHORESE®PROfessional I and II units
9958.1 / 9961.1 ROTIPHORESE®PROfessional runVIEW Base MINI
The runVIEW base MINI consists of a blue light illuminator and a special lid, containing a spectral
emission filter and extractor fan within its viewing pane.
The lid is suitable for dyes with green fluorescence, e.g. the non-toxic dye SYBR®Green, and red
fluorescence, e.g. ethidium bromide or the non-toxic dye ROTI®Gelstain Red (Art. No. 0984).
The illuminator is adjustable to 2 different sizes. It is suitable to the units ROTIPHORESE®
PROfessional I (Art. No. 2788.1) and ROTIPHORESE® PROfessional II (Art. No. 2799.1). No UV
safety equipment required for blue light illumination.
Convenient power supplies are EV1450 (Art. No. CP56.1) or Roth MINI (Art. No. 2907.1).
Operational (runVIEW Base MINI)
USING THE HORIZONTAL GEL ELECTROPHORESIS UNITS
A. Safety Precautions
1. Read the instruction manual thoroughly before use.
2. Never touch the power outlets with any conductive object (e.g. naked metal wire) other than
properly insulated power supply cables.
3. Do not spill liquid or insert metal objects inside the power supply.
4. Never block the ventilation holes or place the unit in any enclosure unless there is adequate
ventilation; never expose the power supply to a direct heat source.
5. Never touch any part of the power supply assembly (i.e. power supply, cables or electrophoresis
tank) before switching OFF the power supply.
6. Never manipulate with wet hands.
7. Do not connect to ground any of the power outputs or the buffer within the electrophoresis tank; the
power outputs should be only connected to an insulated electrophoresis tank equipped with a
safety cover.
8. Do not connect any power supplies in series or in parallel.
9. Never open the back plate nor remove the cover, otherwise an electric shock may result. Repairs
should only be made by the manufacturer or a service technician authorised by the manufacturer.
10.Never use this power supply if the safety cover is not in position correctly.
11.Do not use the unit if there is any sign of damage to the external tank or cover. Contact our
technical service (++49-721-5606-172) immediately to replace or repair any damaged parts.
12.Never use the power supply in the presence of flammable or combustible material as fire or
explosion may result.
13.Ensure that the power supply is only connected to an earthed power line. Do not cut and splice the
power line. When removing the power cord from the wall, unplug it by holding the plug attachment
and not by pulling the cord. Do not hold the plug with wet hands or gloves.
14.Please wear always protective gloves while working.
15.Do not fill the unit with running buffer above the maximum fill lines.
16.Do not move the unit when it is running.
CAUTION: During electrophoresis very low quantities of various gases are produced at the
electrodes. The type of gas produced depends on the composition of the buffer employed. To disperse
these gases, make sure that the apparatus is run in a well ventilated area.
Wave length (excitation)
470 nm (blue)
Ambient temperature
15-40 °C
Current
100 –240 V

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B. General Care and Maintenance
Clean the apparatus with hand warm water and a mild detergent only. Water at temperatures above
60 C can cause damage to the unit and components. Often, a thorough rinse with distilled water is all
that is required. Dry components with clean tissues prior to use, e.g. ROTH tissues (ref. 0087.1).
The tank should be thoroughly rinsed with warm water or distilled water to prevent build-up of salts but
care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or
advised. Air-drying is preferable before use.
Important: Compatible detergents include dishwashing liquid, Hexane and Aliphatic hydrocarbons.
The units should not be left to in detergents for more than 30 minutes.
Acrylic plastic is not resistant to aromatic or halogenated hydrocarbons, ketones, esters, alcohols
(over 25 %) and acids (over 25 %), they will cause „crazing“ of the plastic and should not be used for
cleaning. Do not use abrasive creams or scourers.
The units should never come into contact with the following cleaning agents, these will cause
irreversible and accumulative damage: Acetone, Phenol, Chloroform, Carbon tetrachloride,
Methanol, Ethanol, Isopropyl alcohol, Alkalis.
Before use, and then on a monthly basis, check the unit for any leaks at the bonded joints. Place the
unit on a sheet of dry tissue and then fill with distilled water only to the maximum fill line. If any
leakage is seen do not attempt to repair or use the apparatus, but notify Carl Roth GmbH + Co. KG
immediately (+49/0721/5606-172).
The replacement platinum electrodes are partially shrouded for protection. However, when cleaning
the main tank do not use cleaning brushes in the electrode area.
Ensure that the connectors are clean and dry before usage or storage.
RNAse Decontamination
Clean the units with a mild detergent as described above. Wash with 3 % hydrogen peroxide (H2O2)
for 10 minutes. Rinse with 0.1 % DEPC (diethyl pyrocarbonate)-treated distilled water.
Caution: DEPC is a suspected carcinogen. Always take the necessary precautions when using.
ROTI®Nucleic Acid free (Art. No. HP69) and RNAse AWAY(TM) (Art. No. A998) may also be used.
Please consult the instructions for use with acrylic gel tanks.
Symbols
Indicates the potential for electric shock.
Consult the manual to avoid possible
personal injury or instrument damage.
Indicates disposal instruction.
DO NOT throw this unit into a municipal
trash bin when this unit has reached the
end of its lifetime. To ensure utmost
protection of the global environment and
to minimise pollution, please recycle this
unit.
C. Installation Instructions
Place the runVIEW on a sturdy and level, dry surface. Plug the power cord into the back of the unit
and mains power. Slot the electrophoresis tank so that it fits comfortably on the illumination platform
within the base unit and fit the electrode cables into the lid as follows:
1. Note the position of the lid on the unit. This shows the correct polarity and the correct orientation of
the cables, black is negative and red positive.
2. Remove the lid from the unit. Note if the lid is not removed, fitting the cables may result in un-
tightening of the gold plug and damage to the electrode.
3. Screw the cables into the tapped holes as fully as possible so that there is no gap between the lid
and the leading edge of the cable fitting.
4. Refit the lid.