Roth ROTIPHORESE PROfessional runVIEW User manual

1
Horizontal Electrophoresis System
4849.1 ROTIPHORESE®PROfessional runVIEW System
4850.1 ROTIPHORESE®PROfessional runVIEW Base for
electrophoresis unit ROTIPHORESE®PROfessional III (2850)
9958.1 ROTIPHORESE®PROfessional runVIEW Base MINI 1 for
electrophoresis unit ROTIPHORESE®PROfessional I (2788)
9961.1 ROTIPHORESE®PROfessional runVIEW Base MINI 2 for
electrophoresis unit ROTIPHORESE® PROfessional II (2799)
runVIEW –made by Cleaver Scientific Ltd, UK
Warning: The runVIEW real-time horizontal DNA electrophoresis system has been thoroughly tested
and found to comply within the limits of CE regulation. It has been manufactured using the latest
technology and does not require maintenance. When used correctly this unit poses no particular
health risk, although it can deliver dangerous voltage levels if used incorrectly. Accordingly, this
power supply must only be operated by fully qualified personnel adhering to the guidelines laid
out within this instruction manual. Although this power supply is equipped with all necessary safety
features against abuse and accidental failure, caution should always be exercised when working with
high voltage equipment. Any individual intending to use this instrument should read the entire manual
thoroughly before operation.
The horizontal electrophoresis units from ROTH are designed for long term laboratory use and to
obtain reproducible results. Please spend a few moments reading the instruction manual thoroughly.
These units comply with the statutory CE safety rules:
73/23/EEC: Low voltage directive: IEC 1010-1:1990 plus amendment 1:1992
EN 61010-1:1993/BS EN 61010-1:1993
Please verify that you received the unit completely and without any damage. Any faults or losses have
to be reported to ROTH immediately. ROTH can not accept responsibility for goods that were sent
back without informing them.
Please take a look at the packing list and check whether all components and accessories are present.
Please retain all packaging material until the warranty period has expired.
Environmental Conditions
This unit may only be installed and operated only under the following environmental conditions:
oThis apparatus is intended for indoor use only.
oThe unit can be operated safely at an altitude of 2000 m.
oThe normal operating temperature range is ambient to 40 °C.
oAtmospheric pressure: 75 kPa –106 kPa
oRelative humidity: ≤95 %
This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664.
POLLUTION DEGREE 2, states that: “Normally only non-conductive pollution occurs. Occasionally,
however, a temporary conductivity caused by condensation must be expected”.
All ROTH products are supplied having passed rigorous quality control procedures. For
additional questions please give us a call: ++49-721-5606-0

2
PACKING LIST
4849.1 ROTIPHORESE®PROfessional runVIEW System:
o1x runVIEW base station (including power cord)
o1x special lid with spectral emission filter and extractor fan within its viewing pane
for dyes with green and red fluorescence
o1x ROTIPHORESE® PROfessional III tank w/o lid, with electrodes, 1x 15 x 15 cm gel tray, 2x
casting dams
o1 pair of 4 mm power output cables
o8x double-sided combs:
2x 4-sample/16-sample (1 mm); 4x 20-sample/28-sample (1 mm, multichannel compatible);
1x 4-sample/16-sample (3 mm);1x 20-sample/28-sample (3 mm, multichannel compatible)
oInstruction manual
4850.1 ROTIPHORESE®PROfessional runVIEW
Base:
o1x runVIEW base station (including power cord)
o1x special lid with spectral emission filter and
extractor fan within its viewing pane for dyes with
green and red fluorescence
oInstruction manual
The lid is suitable to electrophoresis unit
ROTIPHORESE®PROfessional III (Art. No. 4850.1)
9958.1
ROTIPHORESE®PROfessional runVIEW Base MINI 1:
o1x blue light illuminator (adjustable to 2 different sizes, including
power cord)
o1x special lid with spectral emission filter and extractor fan within
its viewing pane for dyes with green and red fluorescence
oInstruction manual
The lid is suitable to electrophoresis unit ROTIPHORESE®
PROfessional I (Art. No. 2788.1)
9961.1
ROTIPHORESE®PROfessional runVIEW Base MINI 2:
o1x blue light illuminator (adjustable to 2 different sizes, including
power cord)
o1x special lid with spectral emission filter and extractor fan within its
viewing pane for dyes with green and red fluorescence
oInstruction manual
The lid is suitable to electrophoresis unit ROTIPHORESE®
PROfessional II (Art. No. 2799.1)
runVIEW base station
for PROfessional III unit
runVIEW base MINI 1 with special lid
for PROfessional I unit
runVIEW base MINI 2 with special lid
for PROfessional II unit

3
METHOD
ROTIPHORESE®PROfessional runVIEW for real-time electrophoresis under blue light
ROTIPHORESE® PROfessional runVIEW is an innovative new system designed for real-time size
fractionation and recovery of nucleic acids. You can watch your bands migrate through the gel in blue
light –without mutagen or retina damaging effects.
The runVIEW system maximises the efficiency of DNA recovery from stained gels by minimising the
number of steps involved in post-electrophoretic purification. First choice for rapid gel analysis, gel
elution and all control gels.
TECHNICAL FEATURES
Real-time electrophoresis for ROTIPHORESE® PROfessional III unit
4849.1 ROTIPHORESE®PROfessional runVIEW System
4850.1 ROTIPHORESE®PROfessional runVIEW Base
The runVIEW system consists of a base unit with integrated power
supply and blue LED gel illuminator and a ROTIPHORESE®
PROfessional III unit with a special lid,
containing a spectral emission filter and extractor fan within its
viewing pane.
The lid is suitable for dyes with green fluorescence, e.g. the non-toxic dye SYBR® Green, and red
fluorescence, e.g. ethidium bromide
or the non-toxic dye ROTI®Gelstain Red (Art. No. 0984).
The blue light illuminator provides instant time-saving
visualisation of DNA/RNA band migration. No UV safety equipment required for blue light illumination.
For the ROTIPHORESE® PROfessional III unit, gel trays of 15 x 7, 15 x 10 and 15 x 15 cm are
available. The gel tank or the UV tray may be placed on the viewing platform for immediate
observation of the nucleic acid bands within the gel.
ROTIPHORESE® PROfessional runVIEW is suitable for ROTI®Load DNA Stain, ethidium bromide, and
SYBR®Green stained gels in real-time observation, post-run visualisation, or even post-run staining.
Operational (runVIEW Base)
Wave length (excitation)
470 nm (blue)
Voltage (V)
25 –150 (resolution 1 V)
Max. intensity of current (mA)
300 mA (resolution 1 mA)
Max. output
30 W
Timer
1 –999 mins. (alarm)
Display
LED display
Output type
Constant V or mA
Outlets (parallel socket pairs)
1 (4 mm)
Safety
”no load“ detection
Ambient temperature
15-40 °C
Dimensions (cm)
22 x 29.3 x 8 cm
Weight
2.6 kg
Current
100 –240 V
runVIEW base station with
PROfessional III unit and special lid

4
Real-time electrophoresis for ROTIPHORESE®PROfessional I and II units
9958.1 / 9961.1 ROTIPHORESE®PROfessional runVIEW Base MINI
The runVIEW base MINI consists of a blue light illuminator and a special lid, containing a spectral
emission filter and extractor fan within its viewing pane.
The lid is suitable for dyes with green fluorescence, e.g. the non-toxic dye SYBR®Green, and red
fluorescence, e.g. ethidium bromide or the non-toxic dye ROTI®Gelstain Red (Art. No. 0984).
The illuminator is adjustable to 2 different sizes. It is suitable to the units ROTIPHORESE®
PROfessional I (Art. No. 2788.1) and ROTIPHORESE® PROfessional II (Art. No. 2799.1). No UV
safety equipment required for blue light illumination.
Convenient power supplies are EV1450 (Art. No. CP56.1) or Roth MINI (Art. No. 2907.1).
Operational (runVIEW Base MINI)
USING THE HORIZONTAL GEL ELECTROPHORESIS UNITS
A. Safety Precautions
1. Read the instruction manual thoroughly before use.
2. Never touch the power outlets with any conductive object (e.g. naked metal wire) other than
properly insulated power supply cables.
3. Do not spill liquid or insert metal objects inside the power supply.
4. Never block the ventilation holes or place the unit in any enclosure unless there is adequate
ventilation; never expose the power supply to a direct heat source.
5. Never touch any part of the power supply assembly (i.e. power supply, cables or electrophoresis
tank) before switching OFF the power supply.
6. Never manipulate with wet hands.
7. Do not connect to ground any of the power outputs or the buffer within the electrophoresis tank; the
power outputs should be only connected to an insulated electrophoresis tank equipped with a
safety cover.
8. Do not connect any power supplies in series or in parallel.
9. Never open the back plate nor remove the cover, otherwise an electric shock may result. Repairs
should only be made by the manufacturer or a service technician authorised by the manufacturer.
10.Never use this power supply if the safety cover is not in position correctly.
11.Do not use the unit if there is any sign of damage to the external tank or cover. Contact our
technical service (++49-721-5606-172) immediately to replace or repair any damaged parts.
12.Never use the power supply in the presence of flammable or combustible material as fire or
explosion may result.
13.Ensure that the power supply is only connected to an earthed power line. Do not cut and splice the
power line. When removing the power cord from the wall, unplug it by holding the plug attachment
and not by pulling the cord. Do not hold the plug with wet hands or gloves.
14.Please wear always protective gloves while working.
15.Do not fill the unit with running buffer above the maximum fill lines.
16.Do not move the unit when it is running.
CAUTION: During electrophoresis very low quantities of various gases are produced at the
electrodes. The type of gas produced depends on the composition of the buffer employed. To disperse
these gases, make sure that the apparatus is run in a well ventilated area.
Wave length (excitation)
470 nm (blue)
Ambient temperature
15-40 °C
Current
100 –240 V

5
B. General Care and Maintenance
Clean the apparatus with hand warm water and a mild detergent only. Water at temperatures above
60 C can cause damage to the unit and components. Often, a thorough rinse with distilled water is all
that is required. Dry components with clean tissues prior to use, e.g. ROTH tissues (ref. 0087.1).
The tank should be thoroughly rinsed with warm water or distilled water to prevent build-up of salts but
care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or
advised. Air-drying is preferable before use.
Important: Compatible detergents include dishwashing liquid, Hexane and Aliphatic hydrocarbons.
The units should not be left to in detergents for more than 30 minutes.
Acrylic plastic is not resistant to aromatic or halogenated hydrocarbons, ketones, esters, alcohols
(over 25 %) and acids (over 25 %), they will cause „crazing“ of the plastic and should not be used for
cleaning. Do not use abrasive creams or scourers.
The units should never come into contact with the following cleaning agents, these will cause
irreversible and accumulative damage: Acetone, Phenol, Chloroform, Carbon tetrachloride,
Methanol, Ethanol, Isopropyl alcohol, Alkalis.
Before use, and then on a monthly basis, check the unit for any leaks at the bonded joints. Place the
unit on a sheet of dry tissue and then fill with distilled water only to the maximum fill line. If any
leakage is seen do not attempt to repair or use the apparatus, but notify Carl Roth GmbH + Co. KG
immediately (+49/0721/5606-172).
The replacement platinum electrodes are partially shrouded for protection. However, when cleaning
the main tank do not use cleaning brushes in the electrode area.
Ensure that the connectors are clean and dry before usage or storage.
RNAse Decontamination
Clean the units with a mild detergent as described above. Wash with 3 % hydrogen peroxide (H2O2)
for 10 minutes. Rinse with 0.1 % DEPC (diethyl pyrocarbonate)-treated distilled water.
Caution: DEPC is a suspected carcinogen. Always take the necessary precautions when using.
ROTI®Nucleic Acid free (Art. No. HP69) and RNAse AWAY(TM) (Art. No. A998) may also be used.
Please consult the instructions for use with acrylic gel tanks.
Symbols
Indicates the potential for electric shock.
Consult the manual to avoid possible
personal injury or instrument damage.
Indicates disposal instruction.
DO NOT throw this unit into a municipal
trash bin when this unit has reached the
end of its lifetime. To ensure utmost
protection of the global environment and
to minimise pollution, please recycle this
unit.
C. Installation Instructions
Place the runVIEW on a sturdy and level, dry surface. Plug the power cord into the back of the unit
and mains power. Slot the electrophoresis tank so that it fits comfortably on the illumination platform
within the base unit and fit the electrode cables into the lid as follows:
1. Note the position of the lid on the unit. This shows the correct polarity and the correct orientation of
the cables, black is negative and red positive.
2. Remove the lid from the unit. Note if the lid is not removed, fitting the cables may result in un-
tightening of the gold plug and damage to the electrode.
3. Screw the cables into the tapped holes as fully as possible so that there is no gap between the lid
and the leading edge of the cable fitting.
4. Refit the lid.

6
D. Control interface
There are five buttons and four LED indicators on the faceplate. Each LED indicates the activation
status or mode of operation of the unit.
1. To select Voltage, Current or Time
2. To switch blue light ON or OFF
3. Increase select
4. Decrease select
5. Start / stop the program
a. Setup Mode (before pressing RUN/Start)
Each LED light indicates each activated parameter. For example, the Voltage LED will be activated
when selecting the desired voltage. Use to alternatebetween Voltage, Current and Time.
Use or to adjust the value to the desired setting.
b. Operation Mode
Press to start electrophoresis. The LED light next to the Start/Stop button will light up to
indicate the unit is in operation. Use to monitor the remaining time and changes in current and
voltage.
c. Blue light
There are two modes of blue light illumination for visualisation of nucleic acid bands during
electrophoresis.
1. Press the once to activate the blue light source for 10 seconds, to monitor the extent of
band migration.
2. Press the button for 3 seconds for continuous blue light illumination and real-time
visualisation of band migration.

7
E. Gel Pouring
1. Fit the casting dams over each end of the tray and place onto a level
surface. The dams should be fitted so that there is no gap between the
sides of the tray and the groove in the dams. This will ensure that there
is no possibility of gel leakage.
2. Place the gel casting tray on a flat surface or use the Roth levelling
table (Art. No. N854.1).
3. Insert the appropriate comb into the grooves. Melt agarose in
electrophoresis buffer. Cool the agarose down to 50-60 °C in order to
avoid leaking of the gel during pouring and any damage to the gel
tray. Pour the agarose to the desired height (approx. 5 mm).
4. Do not move the casting tray until the gel has polymerised. We
recommend further polymerisation of the agarose gel for approx. 10 min in the refrigerator. Place
the gel casting tray and agarose gel into the electrophoresis chamber and submerse the gel in
running buffer.
Note: We recommend using 0.5x TBE buffer for optimal signal-to-noise ratio in blue light transmission.
F. Performing real-time nucleic acid separation
1. With runVIEW placed on an even bench surface, switch it on using the ON/OFF button located at
the rear of the base unit.
2. Place the gel tray containing an agarose gel in the middle of the electrophoresis tank in the correct
orientation (the wells in which samples are to be loaded should be closer to the black/negative
electrode)
3. Pour in enough of your electrophoresis buffer so that the gel is just submerged.
4. Load the DNA samples. We recommend use with the DNA loading and staining reagents
ROTI®Load DNAstain1, 2 or 3 containing the non-toxic fluorescent dye EvaGreen®(see below).
5. Select your settings accordingly. In order to run the system at constant voltage, switch the mode
button to the Voltage setting and alter the value to the desired setting as described in Set Up Mode
(the Volt LED will be illuminated by this stage).
6. Use the same principle to run the system at constant current (in this case the Current LED will be
illuminated instead).
7. For separations free of condensation, connect the cable from the runVIEW lid into the rear of the
base to activate the extractor fan (IMPORTANT –see Note, next page).
Note: 1.) In order to operate under constant voltage or constant current modes, adjust the other
parameter to the maximum value. For example, to operate under constant voltage, adjust the current
to the maximum output of 300mA before running the power supply with the voltage set at the desired
output setting. For constant runs, “time” should be set to zero. “Time” then counts up until the
Start/Stop-button is pressed.
2.) We recommend using 0.5x TBE buffer for optimal signal-to-noise ratio in blue light transmission.
To Start the Run
1. Press the button to commence electrophoresis. Press the button again to pause or
stop electrophoresis at any time.
2. Press the button to switch on the blue light source in order to view real-time DNA migration.
In order to conserve the blue light lamp, the light will be there for 10 seconds only and switch off
again by its own. Press the button for 3 seconds to switch constant light on.
3. Once electrophoresis is completed ‘End’ will show in the display accompanied by an alarm.
Press the button again to cancel this.

8
Note:
1.) Blue light bands may only be seen in a darkened room or by shielding the unit from light by a dark
cloth or paper.
2.) In order to conserve the blue light lamp, the light will be there for 10 seconds only, and then switch
off again by its own. Press the button for 3 seconds to switch constant light on.
3.) By its very nature during electrophoresis the application of current through a gel leads to a build up
of heat resulting in the accumulation of condensation within the runVIEW lid viewing pane. Excessive
levels of condensation impair visualisation of the nucleic acid bands within the gel. Condensation may
be cleared by using the fan extractor in runVIEW lid.
Using runVIEW as a blue light illuminator
We recommend use with the DNA loading and staining reagents ROTI®Load DNAstain1 or 2 for blue
light and UV-light excitation.
1. With runVIEW placed on an even bench surface, switch it on using the ON/OFF button located at
the rear of the base unit.
2. Using gloved hands, place the gel tray containing the gel onto the illumination platform within the
base unit and place the orange lid on top.
3. Switch on the blue light by pressing the button located on the front panel. Any present DNA
stained with ethidium bromide or ROTI®Load DNAstain should be immediately visible beneath the
runVIEW lid.
4. Protective glasses are not necessary when viewing the blue light illuminator.
Note:
1.) Blue light bands may only be seen in a darkened room or by shielding the unit from light by a dark
cloth or paper.
2.) In order to conserve the blue light lamp, the light will be there for 10 seconds only, and then switch
off again by its own. Press the button for 3 seconds to switch constant light on.
Using runVIEW for DNA recovery
1. Cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied,
before transferring the blue-light transparent gel tray to the PROfessional III tank located on the
base unit.
2. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the
upper row of wells ('Loading' tier).
3. Replace the lid to connect the PROfessional III tank to the integrated power supply before applying
the voltage as described in SETUP MODE. Press the button to start the run.
4. Press the to switch on the blue LED illuminator.
5. Watch through the runVIEW lid’s viewing pane as the samples migrate in real-time to the second
row of wells ('extraction' tier).
6. Once the DNA bands of interest enter the 'extraction' tier, simply stop the power supply, remove the
lid and harvest the DNA by pipette.
7. Upon harvesting, measure the volume obtained from the extraction well by pipette before
performing ethanol precipitation using 1/10th volume of 3 M Sodium Acetate and 2x volumes of ice-
cold 100 % ethanol. Spin using a micro centrifuge for 10’ at maximum rpm.
8. Decant supernatant and perform a second centrifugation for 10 min. with ice-cold 70 % ethanol.
9. Decant supernatant and dry the DNA pellet at room temperature or using a Speedy vac
10.Once dry, resuspend the pellet in a small volume of distilled water or TE buffer, and store or use
accordingly.

9
TIP: For extractions performed with samples of low DNA amount, a small piece of dialysis
membrane may be inserted into the extraction wells ahead of elution. The paper may be washed by
changing the salt concentration to release the DNA, whereas for the membrane, reversal of the power
output cables in the base unit (i.e. red cable to the black outlet and the black cable to the red outlet)
and application of the voltage for 15-60 seconds, should release the DNA from the dialysis membrane.
For both techniques, the solution should be then removed from the extraction tier and ethanol
precipitation performed as described steps 7-10.
Note:
1.) Blue light bands may only be seen in a darkened room or by shielding the unit from light by a dark
cloth or paper.
2.) In order to conserve the blue light lamp, the light will be there for 10 seconds only and switch off
again by its own. Press the button for 3 seconds to switch constant light on.
3.) By its very nature during electrophoresis the application of current through a gel leads to a build up
of heat resulting in the accumulation of condensation within the runVIEW lid viewing pane. Excessive
levels of condensation impair visualisation of the nucleic acid bands within the gel. Condensation may
be cleared by using the fan extractor in runVIEW lid.
G. At the End of the Run
Turn the power supply settings to zero, turn off mains supply and disconnect the power leads.
Remove safety lid at the end of the run and take gel tray out of the chamber for staining.
Rinse the apparatus with distilled water only after the run (see section: B General Care and
Maintenance). Ensure that the connectors are clean and dry before usage or storage.
NOTE: Make sure to take of current and disconnect the electrophoresis unit from Power Supply
before opening the unit!
H. Accessories for ROTIPHORESE®-PROfessional III electrophoresis unit
Special lid for runVIEW system, green and red fluorescence 3077.1
Gel tray (w/o casting dams) 15 x 7 cm 3250.1
Gel tray (w/o casting dams) 15 x 10 cm 3251.1
Gel tray (w/o casting dams) 15 x 15 cm 3252.1
Gel casting dams (1 pair) 3262.1
Positive electrode 3247.1
Negative electrode 3248.1

10
Combs
Sample volume for a 5 mm thick gel
4 + 2
10
12
16
20
28
35
0.75 mm
91 µl
34 µl
30 µl
20 µl
16 µl
8 µl
7 µl
1.0 mm
122 µl
45 µl
41 µl
27 µl
21 µl
11 µl
10 µl
1.5 mm
182 µl
68 µl
61 µl
41 µl
32 µl
17 µl
15 µl
2.0 mm
243 µl
90 µl
81 µl
54 µl
43 µl
23 µl
20 µl
Wells
4 + 2
10
12
16
20
28
35
Thickness
Art. No.
Art. No.
Art. No.
Art. No.
Art. No.
Art. No.
Art. No.
0.75 mm
3263.1**
3266.1
3269.1
3270.1*
3276.1
3281.1*
3283.1
1.0 mm
3302.1**
3308.1
3309.1
3312.1*
3317.1
3332.1*
3334.1
1.5 mm
3339.1**
3347.1
3350.1
3365.1*
3368.1
3370.1*
3372.1
2.0 mm
3373.1**
3374.1
3378.1
3379.1*
3381.1
3382.1*
3384.1
*compatible with multi-channel pipettors
**comb with especially broad wells + narrow marker well (for preparative gels)
I. Accessories for ROTIPHORESE®PROfessional I electrophoresis unit
Special lid for runVIEW system, green and red fluorescence 3663.1
Power Supply Roth-MINI 2907.1
Power Supply EV1450 CP56.1
Further accessories see Professional I unit, Art. No. 2788.1.
J. Accessories for ROTIPHORESE®PROfessional II electrophoresis unit
Special lid for runVIEW system, green and red fluorescence 3680.1
Power Supply Roth-MINI 2907.1
Power Supply EV1450 CP56.1
Further accessories see Professional II unit, Art. No. 2799.1.
K. Additional Items and Reagents
Levelling table N854
Agaroses:
Standard 3810
NEEO ultra-quality 2267
Agarose-Tablets HP67
Broad Range (for all fragment lengths) T846
GTQ (gene technique quality - for DNA elution) 6352
HR-PLUS (for fragments 100-3000 bp) HP30
High Resolution (for small fragments 50-1000 bp) K297
Low Melt (for gel elution and in-gel-applications, for fragments 500-20000 bp) 6351
LM/PCR (Gel elution of fragments < 1500 bp) HP31
Super LM (particularly low melting temp., for fragments ≥1000 bp) HP54
SynergelTM (Agarose additive for even better band resolution) 0184
Gel Loading Buffers:
ROTILoad DNA 6x (with Glycerol / Ficoll) X904 / X905
ROTILoad DNA short run 6x (with Glycerol) 0095
ROTILoad DNA 1x (with Glycerol) 0100
ROTILoad DNA short run 1x (with Glycerol) 0099
ROTILoad DNA small (with Glycerol) HP03
ROTILoad DNA orange 1 (with Glycerol) HP04
ROTILoad DNA orange 2 (with Glycerol) HP05
ROTILoad DNA tricolor (with Glycerol) HP06

11
Gel Running Buffers:
ROTIPHORESE- 10 x TBE-Buffer 3061
ROTIPHORESE- 10 x TAE-Buffer T845
ROTIPHORESE- 10 x TAE-Buffer light (for gel elution) 0122
DNA Markers:
Please call ++49-0721-5606-0 for our brochure on the complete range of DNA Markers.
Staining Reagents:
Ethidium bromide solution 1 % 2218
Ethidium bromide solution 0.5 % in dropper bottle HP46
Ethidium bromide solution 0.025 % in dropper bottle HP47
Ethidium bromide dye 7870
ROTIGelStain (green fluorescent dye, substitute for eth. bromide) 3865
ROTIGelStain Red (red fluorescent dye, substitute for eth. bromide) 0984
EvaGreen(green fluorescent dye, substitute for eth. bromide) 8905
Methylene blue staining solution 0648
Methylene blue dye A514
SEKUROKA®Decon Bags
(for removal of 125 mg eth. bromide from solutions) T856
3688.1 ROTIPHORESE®PROfessional Flexicaster
External Gel Casting Unit
For use with the electrophoresis units PROfessional I-V
Casting unit for pouring gels outside the gel chamber,
suitable for all PROfessional gel trays up to a size of
26 x 32 cm (W x L).
When using gel trays up to a size of 10 x 10 cm, 2 gels can
be poured simultaneously (PROfessional I and II).
The level bubble allows an optimal levelling of the gels.
Additional sealing is not necessary.
L. Troubleshooting and Maintenance
Each runVIEW system uses all solid-state components and should require no maintenance or
recalibration under normal use. If the unit is to be returned for repair, contact our customer’s service.
Many operating problems may be solved by reading and following the instructions in this manual
accordingly. Some suggestions for troubleshooting are given below. If these suggestions fail to resolve
the problem, contact us for assistance. If troubleshooting service is required, please include a full
description of the problem.
1. Check the troubleshooting section.
2. Call our Technical Service (++49-721-5606-172) or e-mail to [email protected]
3. If it is necessary to return unit for repair, please contact our customer’s service for decontamination
formulas and shipping instructions. The unit will be repaired and returned to you as quickly as
possible.

12
Problem
Cause
Solution
No bands seen in
blue light
No DNA there
Check presence of DNA using a UV illuminator or
methylene blue staining
Too much light present
Darken the room or use a dark cloth or paper to shield
the unit from light.
Lid not used
Bands can only be seen through the orange runVIEW
lid.
Buffer suppresses bands
Faint bands are not seen when using 1x TBE buffer.
Use 0.5x TBE buffer for electrophoresis.
No Display / lights
No AC power.
Check if the power supply is unplugged, or if the AC
power source is a problem.
AC power cord is not
connected.
Check AC power cord connections at both ends. Use
the correct cords.
The fuse has blown.
Replace the fuse
Operation stops
Electrophoresis leads are
not connected to the
power supply or the
electrophoresis unit; or
the circuit is broken in the
electrophoresis system.
Check the connections to the power supply and within
electrophoresis system to make sure the connection
is intact; check the electrodes to make sure they are
intact. Close the circuit by reconnecting the cables.
Press START/STOP to restart the run.
High resistance due to
tape left on a pre-cast
gel, incorrect buffer
concentration, or
insufficient buffer
volumes in the
electrophoresis system.
Make sure that the tape is removed from the pre-cast
gel, that the buffers are prepared correctly, and the
recommended volume of buffer is added to the
electrophoresis unit and is covering the gel.
Error message
Over voltage (170 V
safety limit reached or
exceeded).
Press START/STOP button to clear the error
message. Contact our service department if the
problem persists.
Message
No load is detected.
(1) Check the connections.
(2) Check the buffer condition / buffer
Level.
Alarm message
Maximum power output
reached (30 W).
Warning message for reference.

13
Carl Roth GmbH+Co. KG
Schoemperlenstraße 3-5
76185 Karlsruhe
Postfach 100121
76231 Karlsruhe
Telefon: [phone]
Telefax: (+49)721/5606-149
E-Mail: [email protected]
Internet: www.carlroth.com gh 05/2020
Complete electrophoresis system
ROTIPHORESE®PROfessional runVIEW 4849.1
Electrophoresis Station ROTIPHORESE®PROfessional runVIEW Base
Suitable for: Unit PROfessional III 4850.1
Electrophoresis Station ROTIPHORESE®PROfessional runVIEW Base MINI 1,
Suitable for: Unit PROfessional I 9958.1
Electrophoresis Station ROTIPHORESE®PROfessional runVIEW Base MINI 2,
Suitable for: Unit PROfessional II 9961.1