RWD MP-500 User manual

MP-500
Micropipette Puller
User Manual A

© 2021 RWD Life Science Co., Ltd. All Rights Reserved.
INTELLECTUAL PROPERTY RIGHT
The intellectual property rights of this product and its user manual are owned by RWD Life Science
Co., Ltd. (hereinafter referred to as RWD), including but not limited to patents, trademark rights, and
copyrights.
RWD reserves the right of final interpretation of this user manual.
RWD has the right to treat this user manual as confidential. Without the written permission of RWD,
no individual or organization should disclose this user manual, in whole or in part, by any means, nor
allow others or organizations to access the information of this user manual, in whole or in part, by
any means.
Without the written permission of RWD, no individual or organization should publish, modify, copy,
distribute, rent, adapt or translate this user manual into other languages, in whole or in part.
is the registered trademark or trademark of RWD. The trademark and related security
marks are intangible properties owned by RWD. The use of trademarks or marks not owned by RWD
in this user manual is for no other purposes except for editing, and the rights to them belong to their
respective owners.
STATEMENT
RWD reserves the right to modify this manual without prior notice.
RWD reserves the right to make changes in technology without prior notice.
RWD reserves the right to modify product specifications without prior notice.
RWD provides no guarantee in any form for this manual, including (but not limited to) the implied
warranty of merchantability and fitness for a particular purpose.
RWD considers itself responsible for the safety, reliability and performance of the device only if:
The assembly operations, expansion, readjustment, improvement and repair of this product are
performed by professionals approved by RWD;
The relevant electrical devices conform to the national standards;
The device is used by following the operating instructions.
RWD assumes no liability for the safety, reliability and performance of the product if:
The components are disassembled, stretched or re-debugged;
The device is repaired or modified by an unauthorized person of RWD;
The product fails to be used correctly according to the user manual.

Content
1-Introduction .................................................................................................................... 1
1.1 Overview ............................................................................................................................... 1
1.2 Safety .................................................................................................................................... 1
1.3 Product introduction .......................................................................................................... 1
1.4 Product Features ................................................................................................................. 2
1.5 Product Parameters ........................................................................................................... 3
1.6 Accessories ........................................................................................................................... 4
1.7 Device Conditions ............................................................................................................... 4
2-Important information and security ............................................................................ 5
2.1 Labels .................................................................................................................................... 5
2.2 Safety Cautions ................................................................................................................... 5
2.2.1 General safety ............................................................................................................... 5
2.2.2 Safety Instructions ....................................................................................................... 5
2.3-Safety Function ................................................................................................................... 6
3-Product interface and buttons ..................................................................................... 7
4-Operating Procedures.................................................................................................... 8
4.1 Initial start ............................................................................................................................ 8
4.2 Install glass ......................................................................................................................... 10
4.3 Softening point Test ......................................................................................................... 11
4.4 Edit Parameters ................................................................................................................. 13
4.5 Pull Protocol ...................................................................................................................... 13
4.6 Start and pause ................................................................................................................. 15
4.7 Program List ....................................................................................................................... 15
4.8 Remove microelectrode .................................................................................................. 16
4.9 Pull results .......................................................................................................................... 16
4.10 Preheat jaws .................................................................................................................... 17
4.11 Parameter adjustment ................................................................................................... 18
4.12 System Setting ................................................................................................................ 22
4.12.1 System diagnosis ..................................................................................................... 23

4.12.2 Help ........................................................................................................................... 25
4.12.3 System Maintenance ............................................................................................... 27
5-Troubleshooting ........................................................................................................... 29
6-Maintenance ................................................................................................................. 30
6.1 Overview ............................................................................................................................. 30
6.2 Cleaning and Maintenance .............................................................................................. 30
6.3 Replace filament ............................................................................................................... 30
6.4 Replace Drierite canister .................................................................................................. 35
6.5 General check .................................................................................................................... 37
6.6 Annual inspection ............................................................................................................. 38
7-Warranty ........................................................................................................................ 39

1
1-Introduction
1.1 Overview
First of all, thank you for choosing MP-500 Micropipette Puller made by RWD!
Please be sure to carefully read all the information attached before installing and using this
product for the first time, which will help you use the product better.
RWD Life Science Co., Ltd. is committed to continuously improving product functions and
service quality, and reserves the right to make changes to any product described in this manual
and the content of this manual without prior notice.
For the latest product information, please contact us by telephone or mail, or visit our website
(www.rwdstco.com). Please contact RWD if you find any inconsistency between the actual
product and the information contained herein during the use of the device, or when you have
any questions or suggestions.
Model: MP-500 Micropipette Puller
1.2 Safety
When operating the device, users should read the section
2-Important information and
security
to avoid injury to the operator and damage to the device during use.
If you have any questions or suggestions on safety, please contact us for after-sales service
support.
The device should be operated and managed by trained professionals!
This device is not intended for medical use. Microelectrodes pulled by
this device should be used only for animal experiments in a laboratory
environment, but not be applied on human bodies in any form.
1.3 Product introduction
The Micropipette Puller consists of a heating assembly, a pulley assembly and an air cooling
system. It can make a single capillary glass softened by high temperature heating and then
pulled into two glass microelectrodes with symmetrical tips. It is mainly used by experimental
operators for patch clamp experiments and microinjection operations in the laboratory
environment in universities, research institutions, hospitals and assisted reproductive