RWD RWD-MB-001-A User manual

RWD-MB-001-A
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Microcentrifuge
User Manual D

© 2021 Shenzhen RWD Life Science Co., Ltd,All rights reserved.
Intellectual Property Right
The intellectual property rights of this product and its instruction manual belong to RWD Life
Science Co., Ltd (hereinafter referred to as the RWD), including, but not limited to patent,
trademark, copyright etc.
RWD reserves final interpretation right of this instruction manual.
RWD have the right to use the instruction as confidential information. Any individual and/or
organization shall not disclose the instruction of all or part of the information by any means
without RWD’s written permission. Nor shall any other person or organization be allowed to
obtain all or part of the information of this instruction manual by any means.
No individual and/or organization shall publish, modify, reproduce, issue, rental, adapted, and
translated into other languages without RWD’s writen permission.
is RWD’s registered trademark or mark, these trademarks and the related security
mark belong to RWD’s intangible property. The use of non RWD’s trademark or mark in this
instruction manual are only for editing purposes, without other purposes, the rights belong to their
respective rights owners.
Statement
RWD reserves the right to modify the content of this manual without prior notice.
RWD reserves the right to change the technology without prior notice.
RWD reserves the right to modify the product specifications without prior notice.
RWD does not guarantee the information in any forms, including (but not limited to) the
responsibility of proposing the implied merchantability and suitability for a specific purpose.
RWD in only the following conditions is considered to be responsible for the safety, reliability and
performance of the instruments, i.e.:
Assembly operation, expansion, adjustment, improvements and repairs carried out by RWD
authorized personnel;
Relevant electrical equipment in line with national standards;
The instrument is operated according to the instruction manual.
RWD is not responsible for the products’ safety, reliability or operation status in the following
conditions:
Components are disassembled, stretched or debugged;
Non RWD authorized personnel repairs or alterations to the instruments;
Product may not in accordance with the manual.

Contents
1-Introduction..................................................................................................................................1
1.1 Overview.................................................................................................................................1
1.2 Safety......................................................................................................................................1
1.3 Comprehensive description.....................................................................................................1
1.4 Product features ......................................................................................................................1
1.5 Environmental requirements for equipment............................................................................2
1.6 Product parameters..................................................................................................................2
1.7 Product list..............................................................................................................................2
2-System safety................................................................................................................................3
2.1 Main purposes.........................................................................................................................3
2.2 Personal protection..................................................................................................................3
2.3 Operating restrictions..............................................................................................................3
2.4 Operating safety......................................................................................................................3
2.5 Safety symbols........................................................................................................................3
3-Introduction of product structure..............................................................................................5
4-Product assembling......................................................................................................................7
5- Operation instruction.................................................................................................................8
5.1 Start-up....................................................................................................................................8
5.2 Rotor/Short............................................................................................................................10
5.3 Setup.....................................................................................................................................11
5.3.1 Continuous cooling........................................................................................................12
5.4 Precooling.............................................................................................................................12
5.5 Placement of microcentrifuge tube.......................................................................................13
5.6 Centrifugation.......................................................................................................................13
5.6.1 Preset time/continuous run.............................................................................................13
5.6.2 Short run........................................................................................................................14
5.7 Treatment after operation......................................................................................................14
5.7.1 Remove rotor.................................................................................................................14
5.7.2 Remove microcentrifuge tube........................................................................................14

5.7.3 Leave the microcentrifuge open.....................................................................................14
6-Alarm information.....................................................................................................................15
7-Troubleshooting.........................................................................................................................18
8-Maintenance...............................................................................................................................19
8.1 Considerations ......................................................................................................................19
8.2 Regular maintenance.............................................................................................................19
8.3 Cleaning and disinfection .....................................................................................................20
8.3.1 Clean and disinfect microcentrifuge interior .................................................................20
8.3.2 Clean and disinfect the rotor..........................................................................................20
8.4 Replacement of fuses............................................................................................................20
8.5 Record keeping .....................................................................................................................21
8.6 Parts and materials................................................................................................................21
9-Warranty....................................................................................................................................22

1
1-Introduction
1.1 Overview
First of all, thank you for choosing the Microcentrifuge manufactured by RWD.
Before the installation and application of this product for the first time, please
read all attached materials to help you use this product in a better way.
RWD Life Science Co., Ltd. is committed to continually improving product
features and service quality, and reserves the right to make changes to the User
Manual and any product mentioned herein without prior notice.
For the latest product information, please call or write us or visit our website
(http://www.rwdstco.com/). Please contact RWD in the case of any inconsistency
between actual conditions of product and the User Manual, or any question or
suggestion, during your application of equipment.
This User Manual is applicable to the Microcentrifuge produced by RWD:
Microcentrifuge M1324R
1.2 Safety
When operating the system, please read the “2-System Safety”section
carefully to avoid hurts to operators and damages to the equipment.
If you have any question or suggestion regarding safety, please contact RWD
for after-sales support.
1.3 Comprehensive description
The Centrifuge is a machine used to separate components in a mixture of
liquid and solid particles or liquids via a centrifugal force generated from uniform
circular motion of liquid samples in the rotor driven by a motor. It is mainly used to
separate solid particles from liquid in suspension or two kinds of liquids with
different density and insoluble with each other in emulsion.
Microcentrifuge is mainly used to process liquid samples with a volume of or
less than 1.5/2 mL and needed to be operated at low temperature. It has a speed over
10,000 r/min.
Such product is applicable to daily needs of molecular biology application in
labs, such as nucleic acid kit extraction and tissue protein separation.
1.4 Product features
Quick precooling. Only take 8 min to lower to 4 °C from room temperature
in order to cut waiting time.
Stable temperature control. Stabilize below 4 °C even at the highest speed to
avoid sample inactivation.
This equipment should be operated and managed by
trained professionals!

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Compact size.
Mechanical lock design for the microcentrifuge lid to enable easy opening
and closing.
Touch screen + functional keys. Quick and convenient setting and use of
parameters.
Preset program to enable easy calling during operation.
Small vibration and noise during equipment running. Less interference to
samples and users.
1.5 Environmental requirements for equipment
Please prepare the instrument operating environment according to the conditions
listed below to ensure the operability and safety of the system.
Description
Working environment
Temperature: 5 ℃~ 40 ℃
Humidity: 15% - 75% (non-condensing)
Storage environment
Temperature: -20 ℃~ 60 ℃
Humidity: 15% - 75% (non-condensing)
Air pressure: 50 kPa ~ 106 kPa
Class of pollution: 2
Operating voltage
AC 220V, 50 Hz, rated voltage 3 A, maximum voltage 6 A
1.6 Product parameters
Parameters
Description
Product dimension
≤500 mm ×300 mm ×280 mm
Product weight
≤35 kg
Display size
4.3 inch
Screen pixel
800*480 px
Speed
100-15,000 rpm
Centrifugal force
1g-21,130 g
Centrifugation time
00:30-09:59:59, ∞, 30 s-36,000 s
Set temperature
-10 ℃~ 40 ℃
1.7 Product list
Configuration
Name
Quantity
Description
Standard
Host
1
Microcentrifuge
Standard
Rotor wrench
1
Tool for fitting rotor
Standard
Power cord
1
Power connection
Standard
Waste water tank
1
For storing condensate water
Standard
Rotor
1
M-F24G

3
2-System safety
2.1 Main purposes
The equipment is applicable to daily needs of molecular biology application in labs,
such as nucleic acid kit extraction and tissue protein separation.
2.2 Personal protection
The equipment can only be operated and managed by trained professionals.
Please carefully read the User Manual and get acquainted with the operating steps
before use.
2.3 Operating restrictions
1) It is prohibited to use the equipment in rooms with explosive substances.
2) It is prohibited to use the equipment for processing explosive or highly active
substances.
3) It is prohibited to use the equipment for processing substances that can
producing explosive gases.
2.4 Operating safety
Operation of the equipment should be in strict accordance with the User
Manual. The following general precautions should be followed during operation.
Failure to follow these precautions will be considered as violation of safety
standards and intended use of the equipment. RWD will not be responsible in any
case your misuse of the equipment or failure to follow basic safety requirements.
2.5 Safety symbols
The User Manual and the equipment may contain the following safety symbols
and common signs. If you have any question or suggestion regarding safety, please
contact RWD for after-sales support. Please carefully follow these instructions in
order to prevent equipment damage and prolong service life of the equipment.
Risk of hand injuries
When the microcentrifuge is in use, the rotor will run at high speed. Therefore,
failure to follow the safety precautions and the User Manual will result in risk
of hand injuries.
Warning –general danger zones
When such sign appears on the equipment, the following instruction should be
strictly complied with. Safety precautions on the equipment cannot be ignored.
Warning –Power supply safety
The equipment can only be started when the equipment and the power cord are
not damaged and correctly connected. In case of dangerous situations, the

4
equipment power should be immediately cut off and the plug should be removed
from the equipment or the power socket.
Hazard in flammable environment
It is prohibited to use the equipment in environment with flammable gases
Hazard of electromagnetic interference
Please operate the equipment under controlled electromagnetic environment to
avoid danger from equipment faults. It is prohibited to use signal transmitters
including mobile phones near the equipment. In case of faults and/or needs for
maintenance, please turn off the equipment and contact RWD after-sales service.
The equipment is designed and tested in accordance with group A of CISPR 11 for
Class A devices that emit radio interference as specified in EN 61326-1/EN 55011.
Warning: Class A devices are used in industrial environments. There may be
potential difficulties in ensuring EMC in other environments due to conductive and
radiation harassment in use.
Radiation Hazard
When processing radioactive samples, please follow all applicable radiation safety
procedures. When processing radioactive pollutants, please take appropriate
disinfection and safety measures. Wear protective suit (such as particle protection
mask, gloves and protective shoe covers) according to rules and regulations on
processing of radioactive pollutants in your labs. Dispose radioactive pollutants
based on relevant regulations.
Biological infection hazard
Samples used during intended operations of the equipment can be infectious.
Therefore, it is recommended to follow the general lab regulations on infection
control procedure. Refer to the Laboratory Biosafety Manual of WHO (1984) for
information concerning decontamination media and its use, dilution and effective
application scope. Please comply with all applicable safety procedures when
processing infectious samples. Please take appropriate disinfection and safety
precautions when processing infectious substances. Wear protective suit (such as
particle protection mask, gloves and protective shoe covers) according to infection
control procedures in your labs. Dispose infectious wastes based on applicable
regulations.
Waste treatment
Dispose all debris, wastes and infectious and radioactive pollutants generated from
operations based on application lab regulations. Disinfectants, cleaning fluids and
biological wastes must be disposed according to special waste disposal regulations!
Reagents must be disposed according to MSDSs of their manufacturers.

5
3-Introduction of product structure
Fig. 3-1
Fig. 3-2
1
2
3
4
5
6
7
8
10
11
9

6
S/N
Part name
Description
1
Microcentrifuge lid
Lid
2
Display screen
Display the user interface
3
SHORT button
Start short run
4
START/STOP button
Start/stop centrifugation run
5
OPEN button
Manually open microcentrifuge lid
6
Emergency switch
If the centrifuge cannot open the lid manually after
centrifugation, loosen the emergency switch with a
wrench to force open the lid
7
Fuse holder
For installing/replacing fuse
8
Power On/Off
For controlling power supply
9
Power socket
For providing power output
10
USB port
Software upgrading interface (voltage: 5V)
11
Waste water tank
Collect condensate water

7
4-Product assembling
Note: Please start the microcentrifuge 4 hours after its assembling.
1) Unpack first and take out fittings.
Note: Take out the main body of the microcentrifuge under the cooperation of
two persons by placing both hands under the microcentrifuge and carry it out
together.
2) Place the microcentrifuge onto an appropriate bench and remove the plastic outer
packing.
3) Wait until the equipment temperature rises to the ambient temperature and ensure
all power supplies meet equipment requirements.
4) Connect the microcentrifuge to the power supply and press the power switch to
turn it on. Wait for the display screen to light up and the microcentrifuge lid to
open automatically.
5) Loosen the rotor nuts counterclockwise with rotor wrench, take out the protective
styrofoam bag of the rotor and put the rotor back.
Types of rotors
Description
M-F24G
Maximum speed: 15,000 rpm; capacity: 24 ×1.5 mL; gas tightness
M-F24
Maximum speed: 15,000 rpm; 24 ×1.5 mL
M-F4PCR
Maximum speed: 15,000 rpm; 4 PCR tubes
M-F18Kit
Maximum speed: 15,000 rpm; 18 spin columns

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5- Operation instruction
Operating procedures:
1) Firstly set various parameters such as target centrifugal speed, time of
centrifugation, acceleration mode, ATset mode and target temperature as needed
or directly call preset program before start of experiments.
2) Turn on precooling.
3) After the microcentrifuge reaches the set temperature, loosen the microcentrifuge
lid, put in the microcentrifuge tubes and start centrifugation.
4) After centrifugation, open the equipment and take out the microcentrifuge tubes
from the rotor.
5) Leave the microcentrifuge open, turn off the power and wait until the condensate
inside evaporates.
5.1 Start-up
Press the power button to start the equipment for the first time to enter the main
system interface as follows.
Fig. 5-1
Meanings of interface icons
Type
Icon
Description
This icon shows that Soft mode has been
enabled. At this time, the rotor accelerates
and decelerates gently. This icon disappears
when corresponding function is disabled. In
this case, non-soft mode is started and the

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Status
icon
rotor accelerates and decelerates quickly.
|
ATset mode: indicates the time when
centrifugation timing starts.
indicates that centrifugation timing
starts when operation starts;
indicates centrifugation timing
restarts when 95% of the set relative
centrifugal force or speed is reached.
|
indicates that the highest rotor speed
is the target speed during short run;
indicates that the set speed is the
target speed during short run.
=Sound =Sleep =Auto-open =
continuous cooling
Parameter
setup icon
Click to enter [System Setup] page.
Click to enter [Program] page.
shows target centrifugal force;
shows real-time centrifugal
force. Click to switch to ,
namely to corresponding speed display.
shows target temperature;
shows real-time temperature.
shows target centrifugal duration
shows past centrifugal time;.
Click “+” or “_” to increase or decrease
corresponding set values.

10
Save button. Click to enter [Save Program]
page.
Click once to start [Quick Precooling] and
click again to end [Quick Precooling] or wait
for the rotor cavity temperature to reach
target temperature to automatically end quick
precooling.
Parameter lock button. Start/cancel parameter
lock function. This function is mainly used to
lock homepage parameters to prevent
changes.
5.2 Rotor/Short
Click [System Setup] icon to enter [Rotor] and [Short] setup interface by
default. As shown in the figure, the rotor selected by default is: M-F24G
Fig. 5-2
Click rotor name to enter the following rotor selection interface. This interface shows
parameters of the rotor and you can select by your need and click to save.
Note：Before select the rotor, please make sure that have been replaced the
corresponding rotor.

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Fig. 5-3
After rotor selection is completed, select mode of [Short], which means to select [Set]
or [Max] for short run after click “Short” to start short run.
Be unable to select rotor during centrifuging.
5.3 Setup
Click or to enter the previous or next page in the system setup interface and
click to enable other system functions, such as Sleep, Sound, auto Auto-open or
continuous cooling or click to switch between Chinese and English in the system
language tab. In the following section, the setup of [Continuous Cooling] will be
introduced as an example and setup of other functions will not be explained here.
Fig. 5-4

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Fig. 5-5
5.3.1 Continuous cooling
When select [Continuous Cooling], the rotor cavity temperature can be kept at set
temperature if the following four preconditions are met when the rotor stops
moving.
1) The microcentrifuge is started.
2) The microcentrifuge lid is closed.
3) The set temperature is lower than ambient temperature.
4) The microcentrifuge is not in sleep mode.
When [Continuous Cooling] is not checked, the equipment will keep cooling for 8
hours and then cooling is automatically turned off to enter the sleep mode.
During continuous cooling, the set temperature will be shown on the screen.
Whatever the set temperature, the rotor cavity temperature will not be lower than
4 °C in order to avoid freezing of rotor cavity and the samples as well as forming of
more condensate inside the equipment. Because the rotor is still at such time,
temperature adjustment becomes slower.
If you need to stop cooling, please manually open the microcentrifuge lid.
5.4 Precooling
Click to start precooling. Precooling icon will be displayed at the
real-time temperature button during precooling:

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Fig. 5-6
Click to adjust precooling temperature. When the set
precooling temperature is reached, the cooling stops and the system gives off
intermittent prompt tone to inform operators that the precooling is completed.
Click / [START/STOP] button to stop precooling early.
5.5 Placement of microcentrifuge tube
Vertically remove the rotor, loosen the rotor cover, put in the microcentrifuge
tube, restore the rotor to its original position vertically and tighten the rotor nuts
clockwise with rotor wrench until the rotor is firmly fastened (nuts must be tightened
firmly). Close the microcentrifuge lid.
Note: Don’t put your hands between the microcentrifuge and the lid when
opening and closing the microcentrifuge lid.
5.6 Centrifugation
5.6.1 Preset time/continuous run
Click any parameter block in the main interface including centrifugal force/speed,
temperature or duration to enter the following parameter setup interface.

14
Fig. 5-7
Click parameter value to pop out a numeric keypad for entering a value within the
range prompted in the interface. Here also to set ATset mode and to select the
acceleration mode of Soft or NonSoft.
Preset time and continuous run modes are identical in above parameter setup,
except that for continuous run, ∞ should be selected at duration tab. Manually click
the [START/STOP] button to end the centrifugation process is required when start
continuous run.
After parameter setting, click [START/STOP] button to start centrifugation.
When the set timing reaches the target centrifugation duration, the operation stops
automatically. Click [START/STOP] button to stop preset time run early before the
target duration is reached.
5.6.2 Short run
Press [SHORT] button on the main panel of the centrifugation to start short run
but end it by releasing button. See Section 5.2 for introduction of short run.
5.7 Treatment after operation
5.7.1 Remove rotor
When the microcentrifuge lid opens, rotate the rotor nuts counterclockwise with
rotor wrench to loosen the rotor and vertically take out the rotor.
5.7.2 Remove microcentrifuge tube
Remove the microcentrifuge tube.
5.7.3 Leave the microcentrifuge open
Leave the microcentrifuge open and turn off the power. Wait until the condensate
inside to evaporate.

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6-Alarm information
Alarm information prompt
Alarm reasons
Treatment method
“Operation abnormality!
Wait until the rotor stops and
restart the device. If still
abnormal，contact the after-sales
personnel! ”
The microcentrifuge
lid is forced open
when the face plate
is locked
Wait until the rotor stop moving,
open the lid and then close it,
restart centrifugation.
“Lid cannot be locked!
Reclose the lid or restart the
device. If still abnormal, contact
the after-sales personnel!”
The lid cannot be
locked.
1) Open and then close the lid.
2) Restart the equipment or
contact the after-sales
service.
“Lid cannot be unlocked!
Click "OPEN", restart the device,
or turn off the power supply and
press the emergency unlock button
to open it!”
The lid cannot be
unlocked.
1) Click OPEN to open the lid;
2) Restart the equipment and
again click OPEN to open
the lid;
3) Turn off the power supply
and use the emergency
unlock button to open the lid.
“Device is unbalanced!
Check whether the samples and
rotor are abnomal, or else restart
the device!”
The motor is uneven
with too large
vibration.
1) Check whether the sample is
placed symmetrically;
2) Check whether the rotor is
installed correctly;
3) Restart the equipment.
“Motor stalling!
Restart the device. If still
abnormal, contact the after-sales
personnel!”
The asynchronous
motor stalls.
1) Turn off the equipment and
wait for 5 min before
restarting.
2) If the fault remains, please
contact the after-sales service.
“Hall sensor abnormality!
Restart the device. If still
abnormal, contact the after-sales
personnel!”
Hall sensor is
damaged.
1) Equipment;
2) If the fault remains, please
contact the after-sales
service.
“Refrigeration abnormality!
Restart the device. If still
abnormal, contact the after-sales
personnel!”
Failed to start the
compressor.
1) Turn off the equipment and
wait for 5 min before
restarting;
2) If the fault remains, please
contact the after-sales
service.
“Refrigeration failure!
Contact the after-sales personnel! ”
Compressor
over-current
protection circuit
fault
Contact the after-sales service