RWD DSC-400 User manual

DSC-400
Single Cell Suspension
Dissociator
User Manual A

© 2021 RWD Life Science Co., Ltd. All Rights Reserved.
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Life Science Co., Ltd. (hereinafter referred to as RWD), including but not limited to patents,
trademark rights, and copyrights.
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or in part, by any means, nor allow others or organizations to access the information of this
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whole or in part.
is the registered trademark or trademark of RWD. The trademark and related
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owned by RWD in this user manual is only for the purpose of editing, and the rights to them
belong to their respective owners.
Statement
RWD reserves the right to modify this manual without prior notice.
RWD reserves the right to make changes in technology without prior notice.
RWD reserves the right to modify product specifications without prior notice.
RWD provides no guarantee in any form for this manual, including (but not limited to)
the implied warranty of merchantability and fitness for a particular purpose.
RWD considers itself responsible for the safety, reliability and performance of the device
only if:
The assembly operations, expansion, readjustment, improvement and repair of this
product are performed by professionals approved by RWD;
The relevant electrical devices conform to the national standards;
The device is used by following the operating instructions.
RWD assumes no liability for the safety, reliability and performance of the product if:
The components are disassembled, stretched or redebugged;
The device is repaired or modified by an unauthorized person of RWD;
The product fails to be used correctly according to the user manual.

Contents
1-Introduction......................................................................................................1
1.1 Product Overview.........................................................................................1
1.2 Product Features...........................................................................................1
1.3 Application Conditions.................................................................................2
1.4 Product Parameters.......................................................................................2
1.5 Components..................................................................................................3
2-Important information and security..............................................................4
2.1 Safety Symbols.............................................................................................4
2.2 Safety classification......................................................................................4
2.3 General Safety..............................................................................................4
2.4 Electrical safety............................................................................................5
2.5 Chemical and Biological Safety...................................................................5
3-Product Structure and Interface ....................................................................6
4-Product Assembly.............................................................................................7
5-Operation Guide...............................................................................................8
5.1 Preparation....................................................................................................8
5.2 Start ..............................................................................................................9
5.3 Invoke Program..........................................................................................10
5.4 Run Program...............................................................................................11
5.5 Edit Program...............................................................................................13
5.6 Setting.........................................................................................................16
5.6.1 History..................................................................................................17
5.6.2 Program Import and Export .................................................................18
5.6.3 Date and Time ......................................................................................19
5.6.4 Version..................................................................................................19
6-Alarms.............................................................................................................20
7-Maintenance ...................................................................................................22
7.1 Cleaning and Maintenance.........................................................................22
7.2 Repair .........................................................................................................24
8-Waste disposal ................................................................................................25
9-Warranty.........................................................................................................26

1-Introduction
First of all, thank you for choosing DSC-400 Single cell suspension dissociator made by
RWD!
Please be sure to carefully read all the randomly distributed information before installing
and using this product for the first time, which will help you use the product better.
RWD Life Science Co., Ltd. is committed to continuously improving product functions and
service quality, and reserves the right to make changes to any product described in this
manual and the content of this manual without prior notice.
For the latest product information, please contact us by telephone or mail, or visit our
website (www.rwdstco.com). Please contact RWD if you find any inconsistency between
the actual product and the information contained herein during the use of the device, or you
have any questions or suggestions.
Single cell suspension dissociator Model: DSC-400
1.1 Product Overview
The Single cell suspension dissociator is an automatic tissue processing instrument
designed, developed and produced by Shenzhen RWD Life Science Co., Ltd. and it is
mainly used by researchers, hospitals and third-party testing organizations. It meets the
needs of animal tissue processing in the field of animal cell and molecular biology research
by providing the function of processing animal tissue blocks into single-cell suspension or
homogenate. The prepared single cell suspensions are often used in experiments such as
flow analysis, single cell sequencing or primary cell culture. The preparation of
homogenate is a necessary step for nucleic acid or protein extraction, and the isolated
protein can be used for immunoblotting, ELISA and other experiments, while the nucleic
acid can be used for PCR amplification, and real-time fluorescence quantitative PCR assay.
To avoid injury to the experimenter and damage to the device, please read the section
“2-Important information and security” carefully. If you have any questions or suggestions
on safety, please contact us for after-sales service support.
1.2 Product Features
Dual-use machine enables applying different reagent supplies and procedures to
process tissue into single cell suspensions or homogenates;
Independent operation of multiple channels improves operational efficiency;
User-friendly programming allows the user to invoke default programs or user
programs;
The device should be operated and managed by
trained professionals!

Operation safety ensures that the single cell tube is not loosened or leaked during
operation, and the top components can be disassembled and cleaned.
1.3 Application Conditions
Please prepare the device operating environment under the conditions listed in the table
below to ensure the operation and safety of the system.
Operating conditions
Temperature: 4-35°C (without heater)
4-30°C (with heater)
Humidity: 10-80% RH (non-condensing)
Work in a moist and hot environment: ≤35°C 80% RH
(non-condensing)
Storage conditions
Temperature: -20-70°C
Storage in a moist and hot environment: ≤70°C 93%
RH (non-condensing)
Working power supply
100-240 V AC, 50-60 Hz
Altitude
<2000 m
Others
Keep out of sunlight and rain; prevent severe vibration
1.4 Product Parameters
Device size (L×W×H)
380×215×190 mm
Device weight
9 kg
Display
7" touch screen
Suitable sample weight
20 mg-4000 mg
Suitable buffer
0.3 ml-10 ml
Rotational speed
20-4000 rpm
Rotational direction
Clockwise or counterclockwise
Acceleration
≤100 r/s2
Torque
≤0.15 N.m (at 200-4000 rpm)
Number of channels
4
Maximum number of user programs
200
Noise
≤85 dB

1.5 Components
Accessories
Delivery
Quantity
Purpose
4-channel
dissociator
Standard
1
Preparation of single cell suspensions
and homogenates
Power cord
Standard
1
Plug-in power cords for device power
input, for different countries
Single cell tube
Standard
25/pack
For holding sample tissue and processed
single cell suspensions or homogenates
Enzyme kit
Optional
1
/
User manual
Standard
1
Device operation instruction

2-Important information and security
Before using the device, please first read this chapter carefully and follow all safety
instructions in this manual to prevent injury to the lab personnel or damage to the device.
2.1 Safety Symbols
Identification
Descriptions
High temperature warning: Caution!
High temperature!
Protective grounding
Biological risk
Please consult the manual
Beware of mechanical injury, do not
touch
Danger! High Voltage!
2.2 Safety classification
Electric shock protection grade: I
Waterproof and dustproof grade: IPX0
2.3 General Safety
1) Electrical safety. The applicable electrical safety standard for this device is
IEC61010-1.
2) The device should be placed in a stable, safe and well ventilated conditions, and should
be protected from excessive dust, vibration, strong magnetic field, direct sunlight,
ventilation, excessive humidity or huge temperature fluctuations. The device should be
kept at least 20 cm away from the wall to ensure unblocked ventilation during
operation.
3) The device is only allowed to be used by trained professionals and operated within the
applicable range. Do not use unauthorized accessories or attachments to operate the
device. Non-conforming operation may lead to device damage and personal injury.

4) The altitude should not exceed 2000 m. RWD does not guarantee the device can work
normally at places above 2000 m.
5) Avoid any liquid leaking into the device.
6) Do not operate the device with damaged components.
7) Do not operate the device while the bushings, single cell tubes, shields, and cleaning
tanks are removed.
2.4 Electrical safety
To ensure safely, the following regulations need to be followed:
1) The device must use a matching power supply. If you are not sure of the type of power
supply provided, please consult an authoritative power supplier or local power
company.
2) Use grounding socket to power this device and ensure the ground wire is connected.
Failed to connect to the ground wire may lead to electric shock.
3) To reduce the electromagnetic coupling effect between this device and other device in
the laboratory, please use the power cord supplied with this device. Use of any other
type of power cord is prohibited.
4) Whenever discovered potentially unsafe operation, turn off and unplug the equipment
from the main power supply.
5) Do not open the enclosure without authorization as this may result in electric shock and
damage the device.
6) This device is for indoor use only and should not be exposed to rain or wet conditions.
If liquid comes into contact with any part of the device circuit, please immediately turn
off the power and unplug it; dry the wet part of the device promptly.
7) Ensure that the operating table is dry during the process.
8) When moving the device from a cold environment to a room temperature environment,
condensed liquid droplets may damage the device. Please wait until the device is
dehumidified before operating.
2.5 Chemical and Biological Safety
1) Experimenter should strictly comply with laboratory safety precautions, and wear
protective gloves and protective glasses.
2) When handling hazardous or unknown samples, operate the device in a safety hood for
experimenters ' safety.
3) Before preparing tissue homogenates or single cell suspensions with organic solvents
or caustic substances, be sure to test them for suitability for device components and
single cell tubes.
4) When the device components are contaminated, no one other than the experimenter
should have access to the device.

3-Product Structure and Interface
Figure 3-1
Figure 3-2
No.
Name
Quantity
Function
①
Shield
1
To protect the safety of the
experimenter
②
Bushing
4
Connect channels
③
Indicator light
1
To indicate the power-on status
④
Channel
4
Interface for processing tissue
consumables
⑤
USB-A port
1
Copy of user data
⑥
USB-B port
1
Software upgrade
⑦
Power switch
1
Power on/off the device
⑧
Power interface
1
Connect the power supply

4-Product Assembly
1) Open the packing case and take out the device and accessories.
2) Place the device on a stable operating table and remove the foam packaging.
3) Insert the white bushing into the channel connector of the device, as shown in Figure
4-1.
Figure 4-1
4) Connect the device to the power cord and turn on the power switch to start the device.
Bushing

5-Operation Guide
5.1 Preparation
1) Add the corresponding tissue lysate or enzyme to the single cell tube according to the
kit instructions to mix it.
2) Cut the target tissue sample into small pieces and transfer to the single cell tube. Be
sure to tighten the cover of the single cell tube.
3) Turn the single cell tubes upside down and load into the device channel, as shown in
Figure 5-1. The 4 channels are independent of each other and can process different
samples at the same time.
Figure 5-1

5.2 Start
Turn on the power switch on the rear panel of the device and enter [Home], as shown in
Figure 5-2. When the single cell tube is installed into channels 1, 2 and 3, the channel is in
the state of “Installed”, as shown in Figure 5-2 for channels 1, 2 and 3. When channel 4 is
not installed with a single cell tube, the channel is in the state of “Free”, as shown in Figure
5-2 for channel 4.
Figure 5-2
Name
Description
Click to enter the [Setting] interface, see 5.6 Se
User programs, up to 200
Factory common setups
Click to delete the user program
Click to enter the [Edit Program] interface
User program or default program page turning
User program or default program page turning
Click to clear the program invoked by the selected
channel
Click to assign the selected program to the selected
channel
Click to run the selected channel

Click on a channel to select/cancel it. As shown in Figure 5-3, click on the channel to cancel
it when there is no need to operate on channel 3. Click on the channel to reselect it when it
is necessary to invoke the program on channel 3.
Figure 5-3
5.3 Invoke Program
Click to select [User program] or [Default program] to be invoked on [Home], click
and the selected program will be assigned to the selected channel, as shown
in Figure 5-4. Both channels 1 and 2 invoke programs, channel 3 is unselected and channel
4 is “Free”.
Figure 5-4
Select the channel and click to clear the program invoked by the selected
channel, and the channel status will change to “Installed”, such as Channel 2 in Figure 5-5.
The channel can also reselect the program to replace the cleared program.

Figure 5-5
5.4 Run Program
Select the channel and click . The channel will run the invoked program and
enter the running state, such as channels 1 and 2 as shown in Figure 5-6.
Figure 5-6
Click the channel in running state to pause or end the channel running, as shown in Figure
5-7.
Figure 5-7

Click to pause the running channel, and Channel 1 is “Paused”, as shown in
Figure 5-8. Click again to continue running the channel.
Figure 5-8
Click to manually end the program, and Channel 1 is “Ended”, as shown in
Figure 5-9. Click again to restart the channel.
Figure 5-9
At the end of the channel run, its status changes to “Ended”, as shown in Figure 5-9.

5.5 Edit Program
Click a program in [User Program] or [Default Program] and click to enter the
[Edit program] interface, as shown in Figure 5-10.
Figure 5-10
Click to save the current user program. If the current program is a default
program, the button is not available and the default program cannot be modified.
Click to save as a user program.
Click to preview the current program and enter the [Preview program]
interface, as shown in Figure 5-11.
Figure 5-11
Stage
Step
Step Type
Speed
Duration

1) Step
Click to select [Step], as shown in Figure 5-12.
Figure 5-12
Click / to add a new step to the left/right of the
selected step, as shown in Figure 5-13. Edit the step type, speed, duration and direction of
the new step in the [Add new step] interface.
Figure 5-13
Click in the [Edit program] interface as shown in Picture 5-12 to edit
the step type, speed, duration and direction of the currently selected step.
Click in the [Edit program] interface as shown in Picture 5-12 to delete
the currently selected step.

2) Stage
Click to select [Stage], as shown in Figure 5-14.
Figure 5-14
Click / to add a stage to the left/right of the
selected stage, as shown in Figure 5-15. Edit the cycle time for the new stage and choose
whether to heat it or not in the [Add new stage] interface. The cycle times range from 1 to
10.
Figure 5-15
Click in the [Edit program] interface as shown in Picture 5-14 to edit
the cycle time of the currently selected stage and whether to heat it or not.
Click in the [Edit Program] interface as shown in Picture 5-14 to delete
the currently selected stage.

5.6 Setting
Click on [Home] to enter the [Setting] interface, as shown in Figure 5-16.
Figure 5-16
Name
Function
Language
Switch language to Chinese or English
Brightness
Adjust display brightness
History
Click to view the history, see 5.6.1 History
Program import
and export
Click for program import and export, see 5.6.2
Program Import and Export
Date and time
Click to set date and time, see
5.6.3 Date and Time
Version
Click to view the information of the system
version, see 5.6.4 Version
Manufacturer
maintenance
Only available to RWD after-sales personnel

5.6.1 History
Figure 5-17
The history is shown in green to indicate that the program running is not ended. The history
is shown in red to indicate that the program is ended manually or abnormally.
Click one of the history records to view it and enter the [View the program history]
interface, as shown in Figure 5-18.
Figure 5-18