RWD R500 Series User manual

User Manual V1.0
R500 Series Compact Small Animal
Anesthesia Machine

© 2018 Shenzhen RWD Life Science Co., Ltd,All rights reserved.
Intellectual Property Right
The intellectual property rights of this product and its instruction manual belong to RWD Life
Science Co., Ltd (hereinafter referred to as the RWD), including but not limited to patent,
trademark, copyright etc..
RWD reserves final interpretation right of this instruction manual.
RWD have the right to use the instruction as confidential information. Any individual and
organization shall not disclose the instruction of all or parts of the information by any means
without RWD’s written permission. Nor shall any other person or organization be allowed to
obtain all or part of the information of this instruction manual by any means.
No individual and organization shall but not limited to publish, modify, reproduce, issue, rental,
adapted, and translated into other languages with RWD’s writen permission.
is RWD’s registered trademark or mark, these trademarks and the related security
mark belong to RWD’s intangible property. The use of non RWD’s trademark or mark in this
instruction manual are only for editing purposes, without other purposes, the rights belong to their
respective rights owners.
Statement
RWD reserves the right to modify the content of this manual without prior notice.
RWD reserves the right to change the technology without prior notice.
RWD reserves the right to modify the product specifications without prior notice.
RWD is not to guarantee the information in any forms, including (but not limited to) the guarantee
responsibility of proposing the implied merchantability and suitability for a specific purpose.
RWD only in the following conditions is considered to be responsible for the safety, reliability and
performance of the instruments, i.e.:
Assembly operation, expansion, adjustment, improvements and repairs were carried out by RWD
authorized personnel;
Relevant electrical equipments in line with national standards;
The instrument is operated according to the instruction manual.
RWD is not responsible for the products’ safety, reliability and operation status in the following
conditions:
Components are disassembled, stretched and debugged;
Non RWD authorized personnel repairs or alterations to the instruments;
Product may not in accordance with the manual.

Contents
1- Introduction ....................................................................................................... 1
1.1 Overview ........................................................................................................................1
1.2 Safety..............................................................................................................................1
1.3 Product features .............................................................................................................2
1.4 Equipment Environment Requirements .........................................................................2
1.5 Features of main components of equipment .................................................................3
2- Safety................................................................................................................. 5
2.1 Overview ........................................................................................................................5
2.2 Usage restriction.............................................................................................................5
2.3 Description of safety symbols.........................................................................................5
2.4 safety precautions and practices ...............................................................................5
2.5 System malfunction ........................................................................................................7
2.6 Emergency shutdown .....................................................................................................7
3- Unpacking and assembling ................................................................................. 7
3.1 Overview ........................................................................................................................7
3.2 Unpacking.......................................................................................................................7
3.3 Assembling the system ...................................................................................................8
4-System setup..................................................................................................... 10
4.1 Overview ......................................................................................................................10
4.2 Materials and goods .....................................................................................................10
4.3 Initial system preparation.............................................................................................10
5- Operation......................................................................................................... 15
5.1 Overview ......................................................................................................................15
5.2 Pre-use checkout ..........................................................................................................15
5.3 Anesthesia procedures .................................................................................................15
5.4 Post-anesthesia procedure ...........................................................................................18
6 Troubleshooting ................................................................................................ 19
6.1 Overview ......................................................................................................................19

6.2 Safety............................................................................................................................19
6.3 Machine status .............................................................................................................19
6.4 Record keeping.............................................................................................................19
6.5 Problem –Solution matrix ............................................................................................19
7 Maintenance ..................................................................................................... 22
7.1 Overview ......................................................................................................................22
7.2 Safety............................................................................................................................22
7.3 Annual inspection.........................................................................................................22
7.4 Machine status .............................................................................................................22
7.5 Record keeping.............................................................................................................22
7.6 Parts and materials.......................................................................................................22
7.7 Cleaning the equipment ...............................................................................................22
8 Product information .......................................................................................... 25
8.1 Product warranty..........................................................................................................25
8.2 Product specifications ..................................................................................................25
9 Useful information ............................................................................................ 26
9.1 Overview ......................................................................................................................26
9.2 Pressure equivalents ....................................................................................................26
9.3 Pressure unit conversions.............................................................................................26

1
1- Introduction
1.1 Overview
First of all, sincerely thanks for selecting the R500 series compact small animal
anesthesia machine made by RWD Company.
Please read this instruction manual and all other auxiliary materials carefully before
installing and using the product, which will be helpful to work with it better.
RWD has been always dedicated to improving the product function and the service
quality, and will reserve the rights to revise the product itself and contents described
in the instruction manual at any time without notice in advance.
If you find the practical situations about the supplied goods do not agree with the
contents described in the manual, or have any questions or ideas about our products
and service, welcome to contact us. For latest information, please visit our web site
(www.rwdstco.com) or contact us immediately.
This manual is applicable to:
R500IP compact Small Animal Anesthesia Machine - Isoflurane, Pour Fill
R500IE compact Small Animal Anesthesia Machine - Isoflurane, Easy Fill
R500SP compact Small Animal Anesthesia Machine - Sevoflurane, Pour Fill
R500SE compact Small Animal Anesthesia Machine - Sevoflurane, Easy Fill
1.2 Safetys
Operation of Anesthesia System involves oxygen gas with high pressure and the use
of potentially hazardous materials. In order to prevent injury to animals and/or
operators and damage to the anesthesia system, please read carefully Section 2 -
System security before performing any of the procedures contained in this manual.
If you have any safety questions or advises, please contact us for support.
This small animal anesthesia system should only be operated
and administered by professional personnel.

2
1.3 Product features
Standard open circuit breathing loop design.
Applicable to rats, mice, rabbits, cats, hamsters and other species.
Single breathing circuit design.
The oxygen flow meter adjustable ranges from 0.05 to 1000 ml/min(1L) and
0.1 to 4000 ml/min (4L) .
Oxygen or air is alternative as the gas source.
Pour Fill style anesthetic vaporizer, with capacity ≤120 ml, automatic flow and
temperature compensation.
The concentration of anesthetic gas from the vaporizer can be adjusted(0 -
5%,Isoflurane;0-8%,Sevoflurane）, without effect by fluctuation of flux,
temperature, flow rate and pressure. The safety locking device well prevents
accidental volatilization of anesthetic agent.
The optional expanded flowmeter meets the demand of a variety of gas sources.
With the locking structure for preventing accidental stating and protection
structure in closed state, ensure the system security.
Accessories with various specifications (induction box, mask, anesthetic gas
recovery system etc,) will meet the need of animal with different species and
size.
1.4 Equipment Environment Requirements
Please prepare equipment operation status as Table 1-1 to ensure reliable operation
and safety.
Table 1-1 Equipment Environment Requirements
Detail Description
Gas Supply
Medical oxygen or air pump
Pressure: below 0.42MPa
Working
Environment
Storage temperature：-1 - 55℃
Operating temperature：10-35℃
Relative Humidity：5-90%RH

3
1.5 Features of main components of equipment
Figure 1-2 Equipment main body
No.
Item
Description
1
Handle
Provides hand-hold for steadying the anesthesia system while
it is being moved.
2
O2flowmeter
Used to control the oxygen or air flow that goes into the
anesthesia system. Turn the O2 flowmeter control valve to
regulate the oxygen flow between 0.05~1L/min (1L) and
0.1~4L/min (4L).

4
No.
Item
Description
3
Anesthetic vaporizer
It converts a liquid anesthetic agent into a vapor which is
added to oxygen. The anesthetic vapor is measured in volume
percent (vol %). A dial on top of the vaporizer allows the
operator to select the amount of vapor administered to the
animal.
The vaporizer is the most complicated and expensive part of
the anesthesia system. More attention should be paid to its
maintenance and operation. We suggest that connect with
RWD regularly to calibrate.
Please use the correct anesthetic agent following the
tag on the vaporizer. The misuse may cause the damage to the
equipment and animal.
4
Main Support
Solid metal support for mounting and supporting all of the
above components
5
Anesthesia mask
interface
Used for connecting the anesthesia mask.
6
Gas switch
Pull the gas switch to the direction of the "Chamber", the
anesthetic gas flows to the anesthesia induction chamber. Pull
to the direction of the "Mask", the anesthetic gas to flow to
the anesthesia mask.
7
Anesthesia induction
chamber interface
Used for connecting animal anesthesia induction chamber.
8
O2 flush button
Press to deliver the oxygen or air directly to induction box. It
is generally used while emergency or excluding residual
anesthetic gas in the induction box.
9
Air connection
Used for connecting oxygen or air pumps.

5
2- Safety
2.1 Overview
This section provides safety information necessary for operating our R500 compact
small animal anesthesia machine. If you have any questions or advise, please contact
us for more support.
2.2 Usage restriction
RWD R500 series compact small animal anesthesia machine is only for laboratory or
veterinary use. All the operation and maintenance should follow the instructions of
this manual. Misuse of your system may result in injury to animals and operators
and/or damage to property. Misuse includes:
Using inappropriate gases and anesthetic agents
Making unauthorized modifications to the equipment
Operating the system using gas pressure exceeding maximum ratings
2.3 Description of safety symbols
The following types of symbols are included in this manual with notes that alert the
reader to potential hazards.
2.4 safety precautions and practices
This section provides requirements necessary to ensure safe and reliable operation of
your R500 series compact small animal anesthesia machine.
2.4.1 Animal and operator safety
If possible. please prepare a backup set anesthesia machine in case an emergency occur;
Any person responsible for the installation or operation of the system shall carefully
Property Damage Caution. This symbol appears in a shaded text block to
warn that your actions could cause damage to the system or the facility.
Personal and Animal Safety Warning. This symbol appears in a shaded text
block to warn that your actions could cause personal injury or death.
This small animal anesthesia system should only be operated
and administered by professional personnel.

6
read and familiar with the instruction manual.
To ensure proper function of your system, perform a pre-use checkout procedure in
accordance with Section 5 - Operation.
The system must always be attended by a veterinarian or trained anesthesia technician.
After guarantee period, annual service inspections to ensure proper operation is
recommended. Only RWD-certified technicians should be permitted to service the system
or replace internal parts.
Keep open flames and combustibles (e.g., ether and acetone) away from the anesthesia
system.
Do not place any heavy things on the anesthesia system.
Make sure that the air connection hose is unobstructed to avoid blockage.
Wearing personal protective equipments is suggested.
Make sure the air connection hose is securely connected to the system before starting a
procedure.
Make sure that any exhaled gas containing anesthetic is vented outside of the operating
room or recycled by other proper equipment.
RWD recommends the use of an electrocardiograph, and equipment capable of monitoring
the animal’s pulse, oxygen (O2) saturation, carbon dioxide (CO2) level, and anesthetic
agent level at all times when operating the system.
Remove the system from service if any indications of improper function exist, and contact
RWD.
Never pour any other fluids into the anesthetic vaporizer except the anesthetic agent for
which it is designed, note the identification outside the vaporizer before use.
Avoid use of oil or grease on any anesthesia or respiratory equipment where oxygen is
used. An explosion may occur.
Make sure the pressure of the oxygen is below 0.42MPa。
2.4.2 Preventing system and facility damage
Immediately collect and clean up spilled anesthetic.
Isoflurane is very caustic, avoid it contact with mask and other components if spillage
occurs. If spillage should occur, allow it to evaporate. Do not attempt to wipe it with a
cloth.
Do not place anything on top of the anesthesia equipment.
Follow all recommended system maintenance procedures specified in Section 7 -
Maintenance.
Keep appropriate distance from the wall to ensure a stable gas supply.
Ensure the stability of gas supply.
2.4.3 Environmental health
Immediately collect and clean up any spilled anesthetic agent.
If the system is used in a confined space, ensure that there is adequate ventilation.
Dispose of any hazardous materials and items contaminated with hazardous materials
should be in accordance with local regulations.

7
2.5 System malfunction
If your system does not operate properly, refer to Section 6 - Trouble shooting which
includes descriptions, possible causes, and suggested solutions.
2.6 Emergency shutdown
In the event of a major malfunction, please proceed as follow procedures:
1) Turn off the oxygen supply and the vaporizer；
2) Disconnect the line between the equipment and the air source；
3) Switch the animal to a backup anesthesia delivery system and continue the
experiment.；
4) Contact RWD for after-sale service.
3- Unpacking and assembling
3.1 Overview
This section introduces the unpacking and assembly procedures for your R500 series
compact small animal anesthesia machine.
3.2 Unpacking
Your RWD R500 series compact small animal anesthesia machine has been carefully
packed in our factory for safe delivery to you. When you have received your product,
please do the as follow:
Check the shipping documents to ensure that all boxes have been received.
Examine the shipping boxes for damage. Immediately make a damage claim to
the carrier if there is serious damage and contact RWD. Taking photos is
suggested for keeping the evidence.
Carefully open each box and remove each individual component. Save all boxes
and packing materials for future shipments.
Check the packing list or invoice to ensure all components ordered are included.
In case there is any doubt or need any help, contact RWD or local dealer
immediately.

8
3.3 Assembling the system
3.3.1 Install Vaporizer
Please refer Figure 3-1、3-2、3-3 install vaporizer on main support
Figure 3-1 Twist two screw in the vaporizer, do not twist all in
Figure 3-2 Hang the vaporizer on the screw holes of the main frame
Some components of the system are heavy. Use caution while
lifting these components to avoid injury.

9
Figure 3-3 Use screwdriver to tighten the screw
3.3.2 Vaporizer line connection
Please refer to Figure 3-4 Connect the tube of the anesthesia machine to the gas
INLET and OUTLET of the vaporizer.
Figure3-4 Vaporizer tube connection

10
4-System setup
4.1 Overview
This section provides the setup procedures required to prepare your R500 series
compact small animal anesthesia machine.
4.2 Materials and goods
Oxygen or air source（below 0.42MPa）
Anesthetics (optional, according to the type of vaporizer)
Anesthesia induction chamber（optional）
Anesthesia mask (optional)
The anesthetic gas recovery system (optional)
4.3 Initial system preparation
4.3.1 Setting up the system
1) Move anesthesia machine to use area
2) Connect the oxygen supply or air pump to the inlet port of anesthesia
machine (shown in 错误!未找到引用源。) and make sure the gas supply is
turned off.
Figure 4-1

11
3) Make sure the volume percent dial on the anesthetic vaporizer to the zero
(0) position（Figure 4-2）；
Figure 4-2

12
4.3.2 Fill in the anesthetic
User choose different vaporizer, the anesthetic filling method is also different.
RWD supply two kinds of filling type vaporizer-Pour Fill and Easy Fill .
4.3.2.1 Pour Fill
1) Turn and remove the sealing cap of the vaporizer. Do not leave any debris.
(Figure4-3)
Figure4-3
2) Carefully pour the appropriate agent
into the filler port. Keep the agent level
inside the tube between the two marks.
（Figure 4-4）；
Figure 4-4
3) Tighten the sealing cap（Figure 4-5）
Figure 4-5
Filling for the first time or without use in a long time, please place the vaporizer
for 40 minutes before use.
If anesthetic agent spills on system surfaces, allow it to evaporate. Rubbing
spilled anesthetic agent with a cloth may damage the system finish.
Make sure to pour the correct anesthetic agent into the filler port. Filling with
the incorrect anesthetic agent could cause serious injury or death to the animal.

13
4.3.2.2 Easy Fil
1) Turn and remove the sealing cap
of the vaporizer. Do not leave
any debris. Check the red
sealing ring.（Figure 4-6）；
Figure 4-6
2) screw dosing adapter on the
anesthetic bottle, and insert it
into the vaporizer inlet and
press in the end（Figure 4-7）；
Figure 4-7
3) Carefully pour the appropriate
agent into the filler port. Observe
the agent level sight glass on the
vaporizer.Keep the agent level
between the two marks(Figure4-8)；
Figure 4-8
4) Tighten the sealing cap（Figure
4-9）.
Figure 4-9

14
4.3.3 Connecting the breathing tube
Connect the anesthesia induction chamber(optional) and anesthesia mark(optional)
onto the relevant portal of the anesthesia machine.
Figure 4-10 Breathing tube connection

15
5- Operation
5.1 Overview
This section introduces how to use the R500 series compact small animal
anesthesia machine to handle animal anesthesia experiments after the preparation
works.
5.2 Pre-use checkout
RWD recommend you to check below items before using R500 series compact
small animal anesthesia machine to ensure the stability of the equipment.
Ensure anesthetic agent is filled into the vaporizer.
Make sure the vaporizer volume-percent dial is set to zero (0).
Ensure the oxygen source or air pump is connected with anesthesia system
closely.
Ensure the pressure of air supply is below 0.42MPa and it is enough during
the complete experiment.
Make sure the O2control valve functions normally.
Confirm that the breathing circuit is clear and clean and check the ventilation
and cleanliness of the experimental environment.
5.3 Anesthesia procedures
1) Turn on the oxygen supply or air pump（below 0.42MPa）；
2) Turn the O2 control valve counter clockwise rotation and observe the
float position, adjust to suitable flow rate.
Only trained professionals should operate the anesthesia system.

16
3) Put animal into the induction chamber, push the air switch to the
direction of Chamber, in the meantime, the anesthesia gas flow to the
induction chamber.
4) Press the dial lock key, rotate the evaporator dial, set the vaporizer
concentration to 4-5% until the animals become immobile. To be sure of
complete anesthesia, animals should remain in the induction chamber for
1 minutes at least after initial induction before moving them to the mask.
Pressing of dial lock key is necessary only when the concentration is set
from position “0”.