Sartorius NucleoCounter YC-100 User manual

Cell Counting
NucleoCounter® YC-100™
For total counting of yeast cells and viability
Specifications
Loading volume 60 μl is loaded into the
NucleoCassette
Measurement
volume 1 μl in the measurement chamber of the
NucleoCassette
Analysis time 30 seconds
Measurement
range 5 + 103 to 6 + 106 cells/ml
Size (W + H + D) 38 + 26 + 22 cm,
weight 3 kg
Software NucleoView computer
software for documen-
tation and presentation
– optional
Printer External printer
for documentation
– optional
Description
The NucleoCounter® YC-100™ can be applied
in the counting of yeast cells from both the
pharmaceutical, biotechnology, beer and
food industries.
The NucleoCounter® YC-100™ is very simple
to operate, with only limited training in
laboratory work.
Principle
The NucleoCounter® YC-100™ is an integrated
fluorescence microscope designed to detect
signals from the fluorescent dye, propidium
iodide (PI) bound to DNA. Results from the
NucleoCounter represent either total or
nonviable cell concentration, depending on
the sample preparation.
Key Benefits
– Easy operation
– 30 sec. analysis time
– No cleaning or calibration
– Maintenance and service free
– Excellent reproducibility
– Safe sample handling and disposal
– Excellent for clustering cells
owner of the product

The NucleoCassette™
Propidium Iodide is immobilized in the interior
of the disposable NucleoCassette™. When
the Cassette has been loaded with the cell
lyzate the PI is dissolved and the cellular DNA
is stained.
After placement in the NucleoCounter the
stained mixture is automatically transferred
to the measurement chamber. Green light
excites the PI-DNA intercalation and the red
light emitted is registered in the CCD camera
for correlation into a cell count. After analy-
sis the sample and the PI is contained inside
the NucleoCassette™, which can be safely
discarded. This offers a safe sample disposal.
The thickness of the measurement chamber
of each NucleoCassette™ is measured during
production, accurately determining the
analysed volume in each measurement.
This, together with durable optical compo-
nents, makes the NucleoCounter® YC-100™
calibration free. As the NucleoCassette™
contains the entire flow system as well as the
measurement chamber, neither cleaning nor
maintenance of the NucleoCounter® YC-100™
instrument is needed.
As simple as 1-2-3
Sample Preparation
Mix a representative cell sample with
Reagent Y100 in the ratio 1:10
Sampling
Load the NucleoCassette with the lysate
solution by immersing the tip of the cassette
into the solution and pressing the piston.
Analysis
Place the NucleoCassette in the instrument
and press the “Run” key. After 30 seconds
the cell count is presented on the instrument
display. Optionally data is transferred to an
external PC using USB connection or printed
on an external printer.
Technical specifications subject to change
without notice. Printed in Germany.
Printed on bleached, chlorine-free paper. | W
Publication No.: SL-2077-e13011
Order No.: 85032-544-93
Ver. 01 | 2013
Measurement
chamber
PI-DNA
intercalation
Piston
Green light
from LED’s
Flow channel
system containing
immobilized PI
Red light is
detected in
the CCD
camera
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 11
37079 Goettingen, Germany
Phone [phone]
Fax [phone]
www.sartorius.com
USA Toll-Free +1.800.368.7178
UK +44.1372.737159
France +33.442.845600
Italy +39.055.63.40.41
Spain +34.90.2110935
Russian Federation +7.812.327.5.327
Japan +81.3.5639.9981
Specifications subject to change
without notice. Printed and copyrighted
by Sartorius Stedim Biotech GmbH. | W
Publication No.:
Order No.:
Ver. 01 | 2012