Savant DNA120 User manual

Instruction Manual
Savant DNA120, DNA120 OP
SpeedVac Concentrator
104-3003-00 Rev. C
®

Table of Contents
Section Section Title — Introduction Page
1.0 Introduction 1
Section Section Title — Installation Page
2.0 Installation 1
Receiving 1
Unpacking 1
Inspection 1
Set-up 2
Section Section Title — Operation Page
3.0 Operation 2-3
3.1 Mode Selection 4
3.2 Setting Target Time 4
3.3 Example of Manual Run 5
3.4 Example of Auto Run 6
3.5 Drying Rate 6
3.6 Other Topics 7
Section Section Title — Specifications Page
4.0 Specifications 8
Section Section Title — Accessories Page
5.0 Accessories 9
Section Section Title — Warranty and Page
Liability
6.0 Warranty and Liability 9
Appendix 1:
Proper handling of hazardous exhaust vapors 10
Appendix 2:
Operating hints 10
SPEEDVAC®IS A REGISTERED TRADEMARK OF THERMO ELECTRON CORPORATION, AND IS COVERED UNDER U.S. PATENT NUMBER 4,226,669.
TEFLON®IS A REGISTERED TRADEMARK OF E.I. DuPONT de NEMOURS & CO.
©2000-2003 THERMO ELECTRON CORPORATION
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1.0 INTRODUCTION
The Savant®DNA SpeedVac®Model DNA120, is a dedicated centrifugal
vacuum concentration system for drying low volume ethanol or isopropanol-
water precipitates of DNA and RNA. The Savant DNA120 OP allows for
additional drying down of synthetic oligos in Ammonium Hydroxide
(NH4OH). This design incorporates a glass cover, ammonia post-trap and
Ammonia Neutralizing Solution for fully automated, unattended, odor-free
drying. The post-trap is connected on the back, left side of the unit. It is
easily maintained and replaced. The Savant DNA SpeedVac combines
centrifugal force with vacuum for bump-free sample drying. Vacuum is
supplied by an integral, oil-free vacuum pump with an automatic bleeder
valve.
The Savant DNA120 SpeedVac has a chamber heater that counteracts
evaporative sample cooling and accelerates solvent evaporation rates to
shorten drying times. The operator can select LOW (ambient),
MEDIUM (43 °C), or HIGH (65°C) drying rates with the 3-position switch
on the front panel.
Several different microcentrifuge tube rotors are available for drying ethanol
precipitates of DNA/RNA, isopropanol-water mixtures, amplified reaction
products in buffer, aliquots of radiochemicals, or other low-volume samples
(see section 5). A unique swing-out rotor (RD2MP) for microplates is avail-
able as well.
The Savant DNA SpeedVac is equipped with polypropylene vacuum fittings
to avoid leaks and resist corrosion. A chemical trap kit (DTK120R) and dis-
posable cartridge (DC120R) should be used for trapping volatile radioactivi-
ty when the system is dedicated for drying down radiolabeled materials.
NOTE: To assure safe operation and best results, read this manual in its
entirety before operating this instrument.
2.0 INSTALLATION
RECEIVING Inspect the shipping carton upon receipt. If the carton is
damaged in anyway, do not accept delivery. Contact
Thermo Electron.
UNPACKING Carefully remove the instrument from its shipping carton.
Compare the packing list to the box contents. If there is
a discrepancy, contact Thermo Electron.
INSPECTION Inspect the unit for any damage that may have occurred
during shipment. Should there be any damage, report it
to the carrier and contact Thermo Electron immediately.
Make sure the carrier inspects the damage and leaves an
inspection report. Register any claims for shipping
damage against the carrier or his agent. Save the
shipping carton in the event a return is necessary.
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Approximate Drying Times
Solvent Volume/ Number Time to dryness at drying rate:
Tubes of tubes LOW MEDIUM HIGH
Ethanol 0.5 ml 12 60± 5 min. 35± 5 min. 25± 5 min.
Table 1: Ethanol drying in the Savant DNA SpeedVac. Drying times presented as a
guide only. Each system may vary slightly. The solvents used, and type and quan-
tity of solutes, also effect drying time. Savant DNA SpeedVac Test Conditions:
RD24 rotor with 12 x 1.5 ml tubes.
2.0 INSTALLATION (cont’d)
SET-UP
• Install the system on a stable surface that is clean, dry, level, and
within 4 feet (1.2 meters) of a compatible electrical outlet.
• Connect power cord to receptacle on the left side of the instrument
and plug into appropriate outlet. The cover latch mechanism releases
automatically.
WARNING: Before connecting the DNA120 to an outlet, make certain that
voltage, frequency, and amperage match the DNA120
requirements indicated on the label on the left-hand side of the
instrument (120VAC / 60 Hz, 10 A or 230 VAC / 50 Hz, 5 A).
If there are any questions, please consult an electrician.
• Place rotor on the drive shaft in the sample chamber. Hand-tighten
the hold-down knob until firmly seated.
CAUTION: Use only Thermo Electron designed rotors in the
Savant DNA SpeedVac®.
• For 230 VAC / 50 Hz, 5 A units only: A safety latch prevents
the Savant DNA SpeedVac lid from operating until power is applied to
the system. Do not attempt to open the lid unless the unit is
plugged in.
3.0 OPERATION
The switches on the front panel control operation of the Savant DNA
SpeedVac. The LED readout displays “run” parameters in minutes. You may
choose between three drying rates (LOW/MEDIUM/HIGH).
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A
D
B
C
3.0 OPERATION (cont’d)
ROTOR INSTALLATION. Open the lid of the rotor chamber. Carefully lower
the rotor onto the drive shaft. Secure the assembly by screwing the
retaining knob into the drive shaft above the rotor. Tighten it firmly but not
excessively. Load the rotor and close the lid. Always balance rotor loads.
There need not be a tube in each hole, but the load must be evenly
distributed.
USING THE CONTROL PANEL
A. OPERATING MODE SWITCH (see section 3.1)
AUTO Press to start a run with automatic shut-off
OFF Press to stop any run
MANUAL Press to start a run with manual shut-off
B. TIMER SELECTION SWITCH (see section 3.1)
HEATER Press to select Heater timer
RUN Press to select Run timer
C. CHANGE SETTING SWITCH (see section 3.2)
Press to increase value of timer selected by switch B
Press to decrease value of timer selected by switch B
D. DRYING RATE SWITCH (see section 3.6)
LOW Concentrator chamber at ambient temperature
MEDIUM Maintains chamber at 43°C
HIGH Maintains chamber at 65°C
104-3003-00 Rev. C