Savant DNA120 User manual

Instruction Manual
Savant DNA120, DNA120 OP
SpeedVac Concentrator
104-3003-00 Rev. C
®

Table of Contents
Section Section Title — Introduction Page
1.0 Introduction 1
Section Section Title — Installation Page
2.0 Installation 1
Receiving 1
Unpacking 1
Inspection 1
Set-up 2
Section Section Title — Operation Page
3.0 Operation 2-3
3.1 Mode Selection 4
3.2 Setting Target Time 4
3.3 Example of Manual Run 5
3.4 Example of Auto Run 6
3.5 Drying Rate 6
3.6 Other Topics 7
Section Section Title — Specifications Page
4.0 Specifications 8
Section Section Title — Accessories Page
5.0 Accessories 9
Section Section Title — Warranty and Page
Liability
6.0 Warranty and Liability 9
Appendix 1:
Proper handling of hazardous exhaust vapors 10
Appendix 2:
Operating hints 10
SPEEDVAC®IS A REGISTERED TRADEMARK OF THERMO ELECTRON CORPORATION, AND IS COVERED UNDER U.S. PATENT NUMBER 4,226,669.
TEFLON®IS A REGISTERED TRADEMARK OF E.I. DuPONT de NEMOURS & CO.
©2000-2003 THERMO ELECTRON CORPORATION
104-3003-00 Rev. C

1
1.0 INTRODUCTION
The Savant®DNA SpeedVac®Model DNA120, is a dedicated centrifugal
vacuum concentration system for drying low volume ethanol or isopropanol-
water precipitates of DNA and RNA. The Savant DNA120 OP allows for
additional drying down of synthetic oligos in Ammonium Hydroxide
(NH4OH). This design incorporates a glass cover, ammonia post-trap and
Ammonia Neutralizing Solution for fully automated, unattended, odor-free
drying. The post-trap is connected on the back, left side of the unit. It is
easily maintained and replaced. The Savant DNA SpeedVac combines
centrifugal force with vacuum for bump-free sample drying. Vacuum is
supplied by an integral, oil-free vacuum pump with an automatic bleeder
valve.
The Savant DNA120 SpeedVac has a chamber heater that counteracts
evaporative sample cooling and accelerates solvent evaporation rates to
shorten drying times. The operator can select LOW (ambient),
MEDIUM (43 °C), or HIGH (65°C) drying rates with the 3-position switch
on the front panel.
Several different microcentrifuge tube rotors are available for drying ethanol
precipitates of DNA/RNA, isopropanol-water mixtures, amplified reaction
products in buffer, aliquots of radiochemicals, or other low-volume samples
(see section 5). A unique swing-out rotor (RD2MP) for microplates is avail-
able as well.
The Savant DNA SpeedVac is equipped with polypropylene vacuum fittings
to avoid leaks and resist corrosion. A chemical trap kit (DTK120R) and dis-
posable cartridge (DC120R) should be used for trapping volatile radioactivi-
ty when the system is dedicated for drying down radiolabeled materials.
NOTE: To assure safe operation and best results, read this manual in its
entirety before operating this instrument.
2.0 INSTALLATION
RECEIVING Inspect the shipping carton upon receipt. If the carton is
damaged in anyway, do not accept delivery. Contact
Thermo Electron.
UNPACKING Carefully remove the instrument from its shipping carton.
Compare the packing list to the box contents. If there is
a discrepancy, contact Thermo Electron.
INSPECTION Inspect the unit for any damage that may have occurred
during shipment. Should there be any damage, report it
to the carrier and contact Thermo Electron immediately.
Make sure the carrier inspects the damage and leaves an
inspection report. Register any claims for shipping
damage against the carrier or his agent. Save the
shipping carton in the event a return is necessary.
104-3003-00 Rev. C

2
Approximate Drying Times
Solvent Volume/ Number Time to dryness at drying rate:
Tubes of tubes LOW MEDIUM HIGH
Ethanol 0.5 ml 12 60± 5 min. 35± 5 min. 25± 5 min.
Table 1: Ethanol drying in the Savant DNA SpeedVac. Drying times presented as a
guide only. Each system may vary slightly. The solvents used, and type and quan-
tity of solutes, also effect drying time. Savant DNA SpeedVac Test Conditions:
RD24 rotor with 12 x 1.5 ml tubes.
2.0 INSTALLATION (cont’d)
SET-UP
• Install the system on a stable surface that is clean, dry, level, and
within 4 feet (1.2 meters) of a compatible electrical outlet.
• Connect power cord to receptacle on the left side of the instrument
and plug into appropriate outlet. The cover latch mechanism releases
automatically.
WARNING: Before connecting the DNA120 to an outlet, make certain that
voltage, frequency, and amperage match the DNA120
requirements indicated on the label on the left-hand side of the
instrument (120VAC / 60 Hz, 10 A or 230 VAC / 50 Hz, 5 A).
If there are any questions, please consult an electrician.
• Place rotor on the drive shaft in the sample chamber. Hand-tighten
the hold-down knob until firmly seated.
CAUTION: Use only Thermo Electron designed rotors in the
Savant DNA SpeedVac®.
• For 230 VAC / 50 Hz, 5 A units only: A safety latch prevents
the Savant DNA SpeedVac lid from operating until power is applied to
the system. Do not attempt to open the lid unless the unit is
plugged in.
3.0 OPERATION
The switches on the front panel control operation of the Savant DNA
SpeedVac. The LED readout displays “run” parameters in minutes. You may
choose between three drying rates (LOW/MEDIUM/HIGH).
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3
A
D
B
C
3.0 OPERATION (cont’d)
ROTOR INSTALLATION. Open the lid of the rotor chamber. Carefully lower
the rotor onto the drive shaft. Secure the assembly by screwing the
retaining knob into the drive shaft above the rotor. Tighten it firmly but not
excessively. Load the rotor and close the lid. Always balance rotor loads.
There need not be a tube in each hole, but the load must be evenly
distributed.
USING THE CONTROL PANEL
A. OPERATING MODE SWITCH (see section 3.1)
AUTO Press to start a run with automatic shut-off
OFF Press to stop any run
MANUAL Press to start a run with manual shut-off
B. TIMER SELECTION SWITCH (see section 3.1)
HEATER Press to select Heater timer
RUN Press to select Run timer
C. CHANGE SETTING SWITCH (see section 3.2)
Press to increase value of timer selected by switch B
Press to decrease value of timer selected by switch B
D. DRYING RATE SWITCH (see section 3.6)
LOW Concentrator chamber at ambient temperature
MEDIUM Maintains chamber at 43°C
HIGH Maintains chamber at 65°C
104-3003-00 Rev. C

Actual elapsed time can be viewed during a run or at the end of a run. When
switch A is set to “OFF”, both timers are at 0.00 (0 hours and 0 minutes).
You can change the settings only when switch A is “OFF”; settings cannot
be changed during a run.
Set switch B to “RUN” to view or change the run timer. Set switch B to
“HEATER” to view or change the heater timer.
When you do not set a run time the timer counts upward (time elapsed).
When a time limit is set, the timer counts down; the display indicated the
time remaining.
Using switch C increases or decreases the time setting in 5 minute incre-
ments to to 9.55 (9 hours, 55 minutes) holding down switch C allows you
to rapidly scroll thru the settings until you release the switch.
• When setting the heater timer, above 9.55 (or below 0.05) there
exists a setting of “CCC” (for continuous HEATER operation),
and “OFF” (for HEATER off).
4
3.1 MODE SELECTION
The dual timer front panel permits operation of the Savant DNA120 in one of
the two modes. Select switch A to either:
• MANUAL-to initiate a concentrator run that you can start and stop
manually. The run starts when switch A is set to “MANUAL” when
switch A is set to “OFF”. The unit will end the concentrator run.
or
•AUTO-to start a concentrator run for a predefined time interval. The
run starts when switch A is set to“AUTO”. The run stops
automatically at the end of the predefined time selected. The front
panel maintains two independent timers (switch B).
• RUN-timer measures the duration of the current run. You can view
or change this timer, (using switch C) when switch B is set to “RUN”.
Each of the above consists of a user-specified target setting and a timer of
actual elapsed time.
A three-digit display shows hours and minutes. A decimal point separates
hours from minutes. For example, 9.55 indicates 9 hours and 55 minutes.
The display can be set to show either the setting or the elapsed time.
The decimal point flashes when a run is in progress (auto run or manual run).
The decimal point is always lit when a run is not in progress.
3.2 SETTING A TARGET TIME
104-3003-00 Rev. C

Switch A should be “OFF”.
Set switch B “HEATER” to bring the HEATER time to the display. Adjust
switch C until the display shows the desired setting for HEATER. You can
select:
• “OFF”-to disable the HEATER.
or
• “CCC”-to operate the HEATER for the duration of the run.
or
• A specified length of time. The HEATER switches off at the end
of this time interval. If a longer time than specified run time is
specified, then the HEATER shuts off at the end of the run.
Start the run by setting switch A to “MANUAL”.
During the run, you can toggle the unit to display the following information.
• Elapsed run time. Set switch B to “RUN”. Since there is no
present run time, the display counts upward, showing the hours
and minutes that have elapsed in the current run.
or
• Remaining heating time. Set switch B to “HEATER”. If a
specific time setting for the HEATER is selected, then the
display counts down and shows it.
3.3 EXAMPLE OF A MANUAL RUN
5
104-3003-00 Rev. C

Switch A should be “OFF”.
Before starting the Auto Run, specify, a run time as follows:
Set switch B to “RUN”. Adjust switch C until the display shows the desired
duration of the run in hours and minutes.
Start the run by setting switch A to “AUTO”.
During the run, you can toggle the unit to display the following information:
• Remaining run time. Set switch B to “RUN”. The display now
counts downward, showing the hours and minutes of time left
in the current run.
or
• Remaining heater time. Set switch B to “HEATER”. If a specific
time setting is selected for the HEATER, then the display counts
down and indicates the amount of time remaining before the
HEATER shuts off.
To terminate a run, set switch A to “OFF”.
3.4 EXAMPLE OF AN AUTO RUN
Thermal energy can be applied to the concentrator chamber to counteract the
cooling effect of evaporation, it maintains samples in the liquid state, and
accelerates the concentration run. The DRYING RATE switch selects the
desired drying rate:
“LOW” maintains the concentrator chamber at ambient temperature.
“MEDIUM” maintains the concentrator chamber at 43° C.
“HIGH” maintains the concentrator chamber at 65° C.
Select the highest drying rate if you desire accelerated drying. To ensure
against overdrying of samples, and possible denaturing, it is recommended
that the HEATER timer be set for only a portion of the total run time (e.g.,
50%-75% of total run time).
3.5 DRYING RATE
6
104-3003-00 Rev. C

Input power: 120 VAC / 60 Hz, 10A or
230 VAC / 50 Hz, 5A
Vacuum Chamber: TEFLON®coated aluminum casting
Cover: • Transparent acrylic (DNA120) 230 VAC / 50 Hz
unit include a safety interlock
• Glass cover (DNA120 OP) w/ safety interlock
Drive & Speed: Maintenance-free induction motor; 1725 RPM with
magnetic coupling to rotor drive shaft
RCF approximately 300 xg
Capacity: 24 x 1.5 ml tubes (RD24 rotor)
36 x 1.5 ml tubes (RD36 rotor) or
48 x 0.5 ml tubes (RD48 rotor)
72 x 0.5 ml tubes (RD72 rotor) or
2 microplates (RD2MP rotor)
Chamber temperature: Controlled at ambient, 43° C,
or 65° C with over-temperature safety shut-off
Weight: 86 lbs. (39 kg)
Dimensions (W x D x H): 11 in. x 25 in. x 11 in.
(29 cm x 63 cm x 29 cm)
Pump: oil-free diaphragm
Displacement: 36 l/min at 60 Hz
30 l/min at 50 Hz
Ultimate Vacuum Level: 7 torr (9 mbar)
Registration: ISO 9001-1994. Registered by QMI (Quality
Management Institute). Certificate number 001080.
4.0 SPECIFICATIONS (Subject to change without notice)
7
104-3003-00 Rev. C

RD24 24 x 1.5 ml tubes
RD36 36 x 1.5 ml tubes
RD48 48 x 0.5 ml tubes
RD72 72 x 0.5 ml tubes
RD2MP 2 microtiter plates
ANT100 Post-trap assembly (standard ONLY on DNA120 OP model)
ANS121/4 Ammonia neutralizing solution 4 bottles (for/DNA120 OP)
GSC100 Glass safety cover (standard on DNA120 OP)
DTK120R Chemical Trap Kit for processing radioactive samples; connects
to DNA SpeedVac®exhaust port.Includes SCT120 Chemical
Trap, tubing, DC120R Disposable Cartridge
DC120R Disposable Cartridge for trapping volatile radioactivity
DC120R/4 Disposable Cartridge for trapping radioactivity, package of four
5.0 ACCESSORIES
All Thermo Electron products mentioned in this manual (except glassware)
are warranted against defects in material and workmanship for one year after
the date of delivery to the original purchaser. Thermo’s warranty is limited to
defective materials and workmanship. Warranty work is subject to our
inspection of the unit. No instruments, equipment, or accessories will be
accepted without a Return Material Authorization (RMA) number issued by
Thermo. The warranty obliges you to follow the precautions in this manual.
Under no circumstances shall Thermo be liable for damages due to the
improper handling or use of its products. Thermo assumes no liability,
express or implied, for your use of this equipment.
6.0 WARRANTY AND LIABILITY
104-3003-00 Rev. C

Some drying applications produce exhaust vapors that are hazardous. Even
small volume samples, dried rapidly in quantity in the DNA SpeedVac®, in
aggregate can emit volatile solvents to the atmosphere. Add a post-trap or
a chemical trap.
For samples labeled with radioactivity, Thermo Electron strongly recommends
an adsorbent filter be attached to the DNA SpeedVac exhaust port. This pre-
vents release into the laboratory environment. The DTK120R Chemical Trap
Kit traps volatile radioactivity and organic solvent vapors. Periodically, the
exhaust from the DTK120R Chemical Trap should be monitored for radioac-
tivity to make sure the filter cartridge is not depleted. A typical configura-
tion is shown in Figure 1, shown on page 6.
For questions regarding a specific application, contact Thermo Electron.
APPENDIX 1
PROPER HANDLING OF HAZARDOUS EXHAUST VAPORS
(1) Prewarm the chamber, set switch B “Heater” to med setting 10 to 15
minutes prior to placing samples into the rotor.
(2) When the total volume of sample exceeds 6 to 8 ml per run, a small
amount of aerosol is created at exhaust port. Use the post-trap
assembly to collect the condensate (ANT100).
(3) The DNA SpeedVac is compact enough to place in a laboratory fume
hood, if necessary.
(4) Before using the DNA SpeedVac for applications other than those
recommended, contact Thermo Electron for additional information.
APPENDIX 2
OPERATING HINTS
10
Trapping exhaust vapors containing volatile
radioactivity with the DNA SpeedVac®
Out
Exhaust
Port
located on
left side
Chemical Trap
& Cartridge
DTK120R
In
Change the disposable car-
tridge DC120R every
month if system is used
everyday.
FIGURE 1. DTK120R CHEMICAL TRAP KIT
DNA120
104-3003-00 Rev. C

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