SCIEX C100HT User manual

C100HT Biologics Analyzer System
Installation Guide
June 2018RUO-IDV-09-3829-A

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1 Introduction..............................................................................................................................................4
Hardware Overview...........................................................................................................................................................5
Cartridge............................................................................................................................................................................7
Decommissioning and Disposal..........................................................................................................................................8
Chemical Precautions.........................................................................................................................................................8
2 Installation Instructions.........................................................................................................................10
Unpack and Place the System..........................................................................................................................................10
Unpack the System....................................................................................................................................................10
Unpack the Controller and Monitor...........................................................................................................................10
Remove the Transport Lock..............................................................................................................................................11
Connect the Cables..........................................................................................................................................................12
Configure the Controller and the Software......................................................................................................................13
Set the Instrument ID.......................................................................................................................................................14
Install the Cartridge in the System...................................................................................................................................15
Verify the Alignment of the Transport System ................................................................................................................16
Configure the Photomultiplier Tube (PMT) Voltage.........................................................................................................18
Inject a Sample and Determine the Detector Counts..................................................................................................18
Set the Photomultiplier Voltage.................................................................................................................................19
Test the Detector........................................................................................................................................................20
Review the Default Users.................................................................................................................................................21
Complete the Qualifications.............................................................................................................................................21
3 Troubleshooting.....................................................................................................................................22
Troubleshooting Tables....................................................................................................................................................22
Run a Test Sample...........................................................................................................................................................28
Export Log Files for Troubleshooting................................................................................................................................29
4 Service Kit Bill of Materials...................................................................................................................31
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Contents

WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts
not recommended by SCIEX or use of parts for any purpose other than their intended
purpose can put the user at risk of harm or negatively impact system performance.
CAUTION: Potential System Damage. Follow the instructions in the service documentation
when servicing the system. Do not perform unauthorized procedures.
This guide is intended solely for a trained and qualified Field Service Employee (FSE). It contains information
required to install and troubleshoot the C100HT Biologics Analyzer system.
This capillary electrophoresis system and its components meet or exceed the applicable regulatory requirements.
For safety and regulatory information about the system refer to the C100HT Biologics Analyzer System
Operator Guide.
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1
Introduction

Hardware Overview
Figure 1-1 Front and Side Panel
DescriptionItem
Service door1
Cartridge door2
Sample door3
LED power indicator; green when the system is on, blue when it is off.4
Access cover5
Right side cover6
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Introduction

Figure 1-2 Back Panel
DescriptionItem
Name plate1
FCC Compliance label2
Waste Electrical and Electronic Equipment label3
No User-serviceable Parts label4
For Research Use only label5
RoHS label6
RS-232 connector7
Voltage switch8
Power switch, mains supply connection, and fuse holder9
High Voltage label10
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Introduction

Cartridge
Note: Always allow the cartridge to stand in the cartridge stand at room temperature for at least 20 minutes
before use.
Figure 1-3 Cartridge in Cartridge Stand
DescriptionItem
Gel reservoir1
Cartridge purge port2
Cartridge data key3
Capillary windows4
Cartridge stand5
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Introduction

Decommissioning and Disposal
WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
Before decommissioning, decontaminate the entire system following local regulations. Follow the SCIEX Red Tag
process and complete an instrument Decontamination Form for instrument and parts returns.
When removing the system from service, separate and recycle different materials according to national and local
environmental regulations.
Note: SCIEX will not accept any system returns without a completed Decontamination Form.
Chemical Precautions
WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.
Determine whether decontamination is required prior to cleaning or
maintenance. The customer must decontaminate the system prior to cleaning
or maintenance if radioactive materials, biological agents, or toxic chemicals
have been used with the system.
WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
•Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to
the Safety Data Sheets for the health and safety precautions that must be followed with chemicals. SCIEX
Safety Data Sheets can be found at sciex.com/tech-regulatory.
•Always wear laboratory-required personal protective equipment. For associates, SCIEX recommends the use
of powder-free neoprene or nitrile gloves, safety glasses, and a laboratory coat.
•Avoid ignition sources when working with flammable materials, such as isopropanol, methanol, and other
flammable solvents.
•Take care in the use and disposal of any chemicals. Potential risk of personal injury if proper procedures for
handling and disposing of chemicals are not followed.
•Avoid skin contact with chemicals during cleaning and wash hands after use.
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Introduction

•Collect all spent liquids and dispose of them as hazardous waste.
•Comply with all of the local regulations for the storage, handling, and disposal of biohazardous, toxic, or
radioactive materials.
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Introduction

Unpack and Place the System
Refer to the “Checklists”in the Site Planning Guide for more information.
Unpack the System
WARNING! Lifting Hazard. Make sure that at least three people or a lifting device
are available to lift the CE system. Follow established safe lifting procedures. Refer
to the Site Planning Guide for the weights of system components.
WARNING! Electrical Shock Hazard. Make sure that the system can be
disconnected from the mains supply outlet in an emergency. Do not block
the mains supply outlet.
1. Inspect the shipping box for damage or evidence of mishandling. If external damage is evident, notify the
carrier before opening the crate.
2. Cut the straps on the box and then open the box.
3. Remove the cartridge stand and the ship kit containing cables from the packaging foam.
4. Remove the boxes containing the controller and monitor.
5. (For customers with the cart) Remove the cart from the box and then lock the wheels on the cart.
6. Lift the C100HT Biologics Analyzer system out of the box and then put it on the bench or the cart.
7. Instruct the user to store the packaging in a safe location for later use when moving the system.
Unpack the Controller and Monitor
1. Open the controller carton, remove the controller, and then put it on the cart or a table close to the C100HT
Biologics Analyzer system.
2. Open the monitor carton, remove the monitor, and then put it on the cart or the table close to the controller.
3. Instruct the user to store the packaging in a safe location for later use when moving the system.
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2
Installation Instructions

Remove the Transport Lock
1. Open the sample door.
2. Turn the black knob counterclockwise to remove the transport lock.
3. Instruct the user to store the transport lock in a safe location for later use when moving the system.
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Installation Instructions

Connect the Cables
Required Materials
•In the instrument ship kit box:
•System power cable
•RS-232 cable
•In the monitor carton:
•mDP cable
•Power cable
•In the computer carton:
•Mouse
•Power cable
•Keyboard
•(For customers with the cart) Cart power cable
1. On the back of the system, select the correct voltage. Refer to Figure 1-2.
2. Make sure that the power switch on the back of the system is in the off position.
3. (For customers without the cart) Connect the power cables.
a. Connect one power cable to the back of the system and to an appropriately grounded line voltage outlet.
b. Connect the second power cable to the controller and to an appropriately grounded line voltage outlet.
c. Connect the third power cable to the monitor and to an appropriately grounded line voltage outlet.
4. (For customers with the cart) Connect the power cables.
a. Connect the cart power cable to the cart and an appropriately grounded line voltage outlet.
b. Connect the power cables to the system, the controller, and monitor and then to the power strip on the
cart.
Make sure that the cables are routed above the cross beam on the cart so that they are not pulled when
the cart is raised or lowered.
5. Connect the mDP cable between the controller and the monitor.
6. Connect the keyboard to the controller.
7. Connect the RS-232 cable between the controller and the C100HT Biologics Analyzer system.
8. Press the power switch on the back of the system to turn on the system.
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Installation Instructions

Configure the Controller and the Software
Required Materials
•In the box with the controller: C100HT reprocessing key
•In the instrument ship kit: Customer reference DVD
1. Put the C100HT reprocessing key in a USB port on the controller.
2. Turn on the controller and the monitor and then log into the controller as a user with Windows Administrator
privileges.
The software for the C100HT Biologics Analyzer system is already installed on the controller.
3. Click Start > Control Panel > Date and Time.
4. If necessary, click Change date and time to set the date and time.
5. Click Start > Control Panel > Display > Adjust Resolution. If necessary , set the resolution to 1920
× 1080.
6. In the 32 Karat software, verify the printer for the DataAnalyzer instrument.
a. Go to C:\32Karat and then double-click csmain.exe.
b. In the 32 Karat Software Enterprise window, right-click the DataAnalyzer icon and then click
Open offline.
c. Type C100HTSW in the User name field and C100HTPrivate in the Password field, click
DataAnalyzer in the Project list, and then click Login.
d. Click File > Print Setup. If necessary, click SciexPDF in the Name list and then click OK.
e. Click File > Exit.
7. Verify the printer for the C100HT instrument.
a. In the 32 Karat Software Enterprise window, right-click the C100HT icon and then click Open
offline.
b. Type C100HTSW in the User name field and C100HTPrivate in the Password field, click C100HT
in the Project list, and then click Login.
c. Click File > Print Setup. If necessary, click SciexPDF in the Name list and then click OK.
Do not close the 32 Karat software.
8. Set the size for the electropherogram in the 32 Karat software main window.
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Installation Instructions

CAUTION: Potential Wrong Result. Do not click (Maximize) to maximize any of the
windows in the following step. If any of the windows are maximized, then the title bar
buttons for windows in the DataReviewer software might not be visible.
a. If the 32 Karat software window is maximized, click (Restore).
b. Click File > Open and open a data file.
c. Drag the edges of the data window so that it fills the 32 Karat software window as shown in the following
figure.
9. Click File > Exit to close the 32 Karat software.
10. In the 32 Karat Software Enterprise window, click File > Exit.
11. Put the customer reference DVD in the DVD drive on the controller and then copy the C100HT Biologics
Analyzer Operator Guide to the Windows desktop.
Set the Instrument ID
1. Write down the serial number found in the SN field in the nameplate on the back of the C100HT Biologics
Analyzer system.
2. Start the C100HT software and type Service in the USER ID field, ABsciexService1 in the Password
field, and then click OK.
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Installation Instructions

3. Click (Service) and then click the Maintenance tab.
4. Click Instrument ID.
5. Type the serial number in the Instrument ID field and then click Apply.
Install the Cartridge in the System
CAUTION: Potential System Damage. Handle the cartridge with care. The tips of the
capillaries are made of glass and are fragile. If the tips are broken, then replace the cartridge.
1. If the cartridge has been stored in the refrigerator, it must come to room temperature before use. Follow these
instructions:
a. Remove the cartridge from the storage box and then put it in the cartridge stand.
b. Add enough DDI water to the cartridge stand trough to keep the capillary tips submerged so that they do
not dry out.
c. Allow the cartridge to stand at room temperature for at least 20 minutes before use.
2. Remove the cartridge from the cartridge stand.
3. Remove the seal from the cartridge purge port and then, using a laboratory tissue, wipe off any gel residue.
Refer to Cartridge.
Store the seal in a safe location for later use when storing the cartridge.
4. Open the cartridge door and then put the cartridge in the C100HT Biologics Analyzer system so that the label
faces the front of the system.
5. Put the data key in the socket in either orientation and then close the cartridge door.
If the Latch cartridge automatically option is selected in the C100HT software Settings, then the cartridge
automatically engages and the optical and pressure systems are linked to the cartridge.
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Installation Instructions

The Cartridge Status panel in the C100HT software updates to display the cartridge type, ID, remaining runs,
remaining normalization runs, status, and other information.
Verify the Alignment of the Transport System
Required Materials
•96-well plate
•Empty buffer plate
WARNING! Personal Injury Hazard. Keep fingers clear of the buffer plate carrier and
the sample door. When the sample door is open for more than five minutes, the
tray automatically moves to the park position.
CAUTION: Potential System Damage. Move the transport system one step at a time when
performing the alignment. If the vertical or horizontal position is changed too quickly, the
buffer plate or 96-well sample plate might collide with the capillary tips and damage them.
1. Log into the C100HT software as the service user. Type Service in the USER ID field, ABsciexService1
in the Password field, and then click OK.
2. In the C100HT software, click to go to Service mode.
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Installation Instructions

3. Click the Maintenance tab and then click Start.
4. Click Init to initialize and then put the 96-well plate and the buffer plate in the system.
5. Verify the M position.
a. Click Mand then verify that the transport system moves to the M row of the buffer plate.
b. Click Up and then verify that the capillary tips are centered in the wells.
If the tips are not centered, then click Back or Forward until the tips are centered and then write down the
value in the Set X(M) field.
6. Verify the D position.
a. Click Dand then verify that the transport system moves to row D of the 96-well plate.
b. Click Up and then verify that the capillary tips are centered in the sample wells and that they do not touch
the bottom of the wells.
•If the tips are not centered, then click Back or Forward until the tips are centered and then write
down the value in the Set X(D) field.
•If the capillary tips touch the bottom of the wells, then click Down to lower the height of the 96-well
sample plate and then write down the value in the Set Z field.
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Installation Instructions

7. Finish the alignment.
•If no adjustments are required, then the transport system is aligned. Click Cancel and then click Yes in
the message to confirm.
•If changes are required, go to step 8.
8. Type the new value in the SET X(D) field and then click SET X(D). Do the same thing for the SET X(M)
and SET Z fields, and then click Save Positions.
A message that the EEPROM values have been saved opens.
Configure the Photomultiplier Tube (PMT) Voltage
The photomultiplier voltage must be configured when the system is installed and if the photomultiplier is replaced.
Inject a Sample and Determine the Detector Counts
Required Materials
•12-well tube strip
•20 µL pipette
•Calibration solution
•C100HT Glycan Labeling and Analysis buffer tray
1. Remove the buffer tray from the refrigerator and then allow it to stand at room temperature for at least 20
minutes before use.
2. Remove the seal from the buffer tray, put it in the buffer plate, and then put the buffer plate into the buffer
plate carrier in the C100HT Biologics Analyzer system.
3. Pipette 20 µL of the calibration solution into each well of a 12-well tube strip and then put it in Row A of the
sample tray. Make sure that the solution is at the bottom of the well and there are no bubbles.
4. In the C100HT software, click to go to Service mode.
5. Click Maintenance > Long Fill and then click Start.
The filling process takes approximately 3 minutes.
6. Click Terminal and set the parameters as shown in the following figure.
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Installation Instructions

7. Click Send to instrument.
The injection begins.
8. When the sample injection is complete, test the detector. Refer to Test the Detector. The raw counts should
be between 14 000 and 15 000.
•If at least one of the capillaries has a raw count that is approximately 15 000, then no changes are required.
•If none of the capillaries has a raw count that is approximately 15 000, then set the photomultiplier voltage.
Refer to Set the Photomultiplier Voltage.
Set the Photomultiplier Voltage
1. Start the C100HT software and type Service in the USER ID field, ABsciexService1 in the Password
field, and then click OK.
2. In the C100HT software, click to go to Service mode.
3. In Service mode, click the Terminal tab.
4. Check the voltage.
a. Select Direct Commands.
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Installation Instructions

b. Type RPMT and then click Send to instrument.
The voltage appears below the command.
5. Change the voltage. Type WRPMTV <XXX> where <XXX> is the new voltage and then click Send to
instrument.
Type a value for the voltage that is based on the raw counts reported in the detector test. Refer to Test the
Detector.
•If the raw counts are greater than 15 000, then enter a voltage that is 5 V less than the reported voltage.
•If the raw counts are less than 15 000, then enter a voltage that 5 V more than the reported voltage.
6. Test the detector. Refer to Test the Detector.
•If at least one of the capillaries has a raw count that is approximately 15 000, then no changes are required.
Write down the voltage.
•If there are no capillaries with a raw count that is approximately 15 000, then repeat step 4 and step 5
until the raw counts in at least one of the capillaries is approximately 15 000. Write down the voltage.
Test the Detector
Use this test to make sure that all of the capillaries in the C100HT Biologics Analyzer system work correctly.
1. In the C100HT software, click to go to Service mode.
2. In Service mode, click the System Check tab.
3. Click Detector Test and then click Start.
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Installation Instructions