scigiene SP-850055 User manual

Advanced pH Meter &
Advanced pH Meter Kit
SP-850055 & SP-850056
Instruction Manual

Advanced pH Meter SP-850055 &
Advanced pH Meter Kit SP-850056
Copyright ©2015 by
Sper Scientific
ALL RIGHTS RESERVED
Printed in the USA
The contents of this manual may not be reproduced or transmitted in any
form or by any means electronic, mechanical, or other means that do not
yet exist or may be developed, including photocopying, recording, or any
information storage and retrieval system without the express permission
from Sper Scientific.
INTRODUCTION
Thank you for purchasing the Advanced pH Meter. This meter
reads the pH (balance of alkalinity and acidity) and ORP (oxidation
reduction potential) in the determination of water quality in labs,
industrial and municipal waste water aquariums, fish hatcheries,
environmental testing and other applications.
FEATURES
•Multi-display LCD screen
•N.I.S.T. buffer recognition
•5 points pH calibration
•Hold function
•Maximum and minimum
•Backlight for dark environment operation
•Easy to view probe calibration data
•“Ready”icon on LCD display indicates stability for reading
•USB connection for online logging and uploading 99 memories
to PC for analysis
•Automatic or manual temperature compensation
•External power adapter (optional) for long use testing
•Auto power off to save battery
2

TABLE OF CONTENTS
1. INTRODUCTION . . . 2
2. FEATURES . . . 2
3. LCD DISPLAY & KEYPAD. . . 4
4. SETUP MODE. . . 5
P1.0 Memory Transmission . . . 5
P2.0 Clear Memory . . . 6
P3.0 View Slope & Offset (pH Probe) . 6
P4.0 Calibration Buffer / Select Buffer. 7
P5.0 Ready Icon. . . 8
P6.0 Temperature Units . . . 8
P7.0 Read Time Clock Setting. . . 9
P8.0 Reset. . . . 10
5. PROBE CALIBRATION . . . .10
6. MEASUREMENT PROCEDURES . . . .12
pH Measurement . . . .12
mV Measurement (± 499 mV) . . . . 13
ORP (mV) Measurement (±1999 mV). . 13
Temperature Compensation . . . . 13
Hold Function . . . .14
Record Memory . . . .14
Recall Memory. . . . 14
Recall Minimum & Maximum . . . .15
Backlight. . 15
Auto Power Oႇ.15
7. MAINTENANCE . . . . 16
8. TROUBLESHOOTING .18
9. ERROR CODES. . . . 18
10. PC CONNECTION . . . . 19
11. POWER SUPPLY . . . .21
12. APPENDIX . . . .22
13. SPECIFICATIONS . . . .22
14. WARRANTY . . . .24
3

LCD DISPLAY
LCD display indicates pH,
mV, ORP (mV).
Icon pH, mV indicates the
unit of measure displayed.
READY indicates the
reading is stable.
MAX, MIN indicate maximum or minimum memory value.
HLD Holds the current reading on the display.
REC indicates the meter is in recall mode.
The two digits to the right of °C °F on the display indicate the total
number of records that contain stored data.
The are real time Y-M-D or H:M:S.
ATC meansthe meterisin automatictemperaturecompensation mode.°C/°F
are temperature units of measure
CAL indicates the meter is in calibration mode.
KEYPAD
Press to turn meter on/off. Press and hold for
more than 1 second to enter SET mode.
Switch between NORMAL and CALIBRATION
mode Press to enter manual temperature
setting. In calibration, setting or recall mode, press
to return to normal.
Press to freeze reading.
Press again to release.
Press for more than 1 second to switch
between NORMAL and RECALL mode.
Press to switch mode.
Press to increase setting value.
Press to save current reading.
Press to decrease the setting value.
Press to confirm calibration/parameter setting.
Press to view the min/max of
the memory in recall mode.
4

SETUP MODE
The advanced Setup Mode allows you to customize the following
meter preferences and defaults:
•P1.0 Memory Transmission
•P2.0 Clear Memory
•P3.0 Electrode (pH probe)
•P4.0 Buffer Solution (pH)
•P5.0 Ready Function
•P6.0 Temperature Units
•P7.0 Real Time Clock
•P8.0 Reset
To enter Setup Mode, press SET for >2 seconds while the meter is in
Normal Mode.
Note...
To exit Setup Mode without saving, press ESC until the Normal Mode
appears. If the meter is in Setup Mode, press ESC twice to exit.
P1.0 Memory Transmission
To transfer stored data from the meter to the computer:
1. Connect the USB cable to the right side of the meter, then
connect the other end of the cable with the D-sub connector
to the computer’s serial port. Run the software associated with
this feature.
2. Press SET for 2 seconds to enter setup.
The “TR”icon appears on the middle of
the LCD display and P1.0 displays
under the “TR”icon.
3. Press MN/MX/AV. The “OUT”icon flashes
on the upper display and P1.1 displays
under the “OUT”icon. This indicates
that the memories are transferring. After
transmission, the LCD will return to P1.0.
Note...
The meter can store up to 99 records for each parameter. If you want to
transmit data for a different parameter, press ▲to select your
parameter before entering setup.
5

P2.0 Clear Memory
1. Press MODE to select the parameter you want cleared before
entering Setup Mode.
2. Press SET for 2 seconds to enter setup.
Press ▲to select memory clear function.
3. The “CLR”icon appears on the
middle display and P2.0 illuminates in
the lower display.
4. Press MN/MX/AV to enter P2.1.
The default “NO”icon flashes on the
middle display and P2.1 appears in the
lower display.
5. Press ▲to change the status from “NO”
on The display to “YES”and then press
MN/MX/AV again to confirm clearing of
all memory. The LCD display will return
to P2.0 when memories are deleted.
CAUTION:
THE MEMORY CLEAR PROGRAM IS DESIGNED TO
CLEAR 99 MEMORIES AT ONE TIME. PLEASE CONSIDER
CAREFULLY IF YOU DECIDE TO CLEAR THE MEMORY AS
THIS OPERATION CANNOT BE REVERSED.
P3.0 View Slope & Offset
1. Press MODE to select probe type as pH.
2. Press SET for 2 seconds to enter setup.
3. Press ▲until the icon “ELE”appears in
the middle display and P3.0 appears in
the lower display.
6

4. Press MN/MX/AV to enter P3.1, the LCD displays one of four
available slope values; P3.1, P3.2, P3.3, P3.4. If the value is less
than 75% or more than 115%, change the probe immediately.
5. Press MN/MX/AV to enter P3.2, P3.3, and P3.4.
Note...
The solution range definition differs between N.I.S.T. and
Custom buffers.
6. Press MN/MX/AV to enter P3.5 and view
the offset value. The offset value is the mV
value of pH 7 and the default offset value
is 0.0. The offset value will be different
after calibration. If the value is outside
the range of ± 60 mV, replace the probe.
P4.0 pH Calibration Buffer
This meter allows the selection of two different types of pH buffers:
N.I.S.T. or CUSTOM. Selection of the proper buffer type more
accurately calibrates the probe to specific requirements.
N.I.S.T. buffer: (five settings)
pH 1.68, 4.01, 6.86, 9.18, 12.45
CUSTOM buffer: (five ranges)
pH 1.00-3.00, 3.50-5.50, 6.00-8.00, 8.50-10.50, 11.50-13.50
7

Select Buffer
1. Press SET for 2 seconds to enter setup.
2. Press ▲to select pH buffer. “BUF”will
appear on the middle of the LCD and
P4.0 will appear on the lower portion.
3. Press MN/MX/AV to enter P4.1. The
default “NIS”(N.I.S.T.) will flash on the
LCD and P4.1 will appear on the lower
portion of the display. If you use N.I.S.T.
buffers, press MN/MX/AV to confirm
and the meter returns to P4.0.
4. If your requirement is not for N.I.S.T.
buffers, press ▲to change the status
to CUSTOM buffer.
5. Press MN/MX/AV to confirm and the
meter will return to P4.0.
P5.0 Ready Icon
This feature enables/disables the
“READY”icon, which indicates that
the measured reading is stable.
1. Press SET for 2 seconds to enter setup.
2. Press ▲to select “READY”on
the display.
3. Press MN/MX/AV to enter P5.1. “YES”
will flash on the LCD display and P5.1
will appear on the lower display.
4. Press ▲to switch between YES or NO.
5. Press MN/MX/AV to confirm.
6. Press ESC to return to Normal Mode.
P6.0 Temperature Units
To select either Celsius or Fahrenheit temperature scale:
1. Press SET for 2 seconds to enter setup.
2. Press ▲to select “U”on the upper
display. P6.0 is displayed in the lower
portion of display. 8

3. Press MN/MX/AV to enter P6.1. The
last selected unit “C”or “F”will appear
on the LCD.
4. Press ▲to select either display.
5. Press MN/MX/AV to save the selection.
6. Press ESC to return to Normal Mode.
P7.0 Real Time Clock Setting
This procedure adjusts the meter’s internal clock. An internal battery
powers the real time clock independent of the power source running
the meter.
1. Press SET for 2 seconds to enter setup.
2. Press ▲to select “RTC”on the LCD display. P7.0 appears
on the bottom of the display.
3. Press MN/MX/AV to enter P7.1. The year flashes in the lower right
corner of the LCD display. (The year is the last two digits only; for
example, 1999 would be 99).
Symbol: Y-M-D H:M:S
Definition: Yr.-Mo.-Day Hr.-Min.-Sec.
Range: 99-12-31 23-59-59
4. Press MN/MX/AV to step through the following “P’s.”All are
two digits.
P7.1 = Year P7.2 = Month P7.3 = Day
P7.4 = Hour P7.5 = Minute P7.6 = Seconds
5. Press ▲and ▼to adjust values up or down, respectively.
6. Press ESC to return to P7.0 and Normal Mode.
9

P8.0 Reset
This procedure will reset the meter to factory default settings.
Memory locations are not reset after this procedure.
1. Press SET for 2 seconds to enter setup.
2. Press ▲to select the reset section
of the meter.
3. “rSt”will appear on the LCD and P8.0
will appear directly below.
4. Press MN/MX/AV to enter P8.1.
5. Press ▲to switch between
“YES”or “NO.”
6. Press MN/MX/AV to confirm.
7. Press ESC to return to Normal Mode.
PROBE CALIBRATION
Calibrate routinely before use, if the readings seem erratic, and
whenever the batteries are replaced. For the highest accuracy, we
recommend a two point calibration. If only calibrating at a single
point, make certain that the buffer value is close to the sample that
will be measured and the buffer temperature remains stable.
1. Press POWER to turn the meter on and press MODE to select
“pH”mode. “pH”appears on the LCD.
2. Select the calibration buffer type: N.I.S.T. or CUSTOM
(see page 14).
Note…
pH 4 may be calibrated using either the N.I.S.T.(recommended
option) or CUSTOM buffer. pH 7 and pH 10 require the CUSTOM
buffer. (See SETUP MODE on page 14.)
3. Rinse the probe in de-ionized water or rinse solution. DO NOT
wipe the pH probe dry. Wiping the probe may cause static and
cause calibration and measurement instability.
4. Select the pH buffer and pour enough solution to totally immerse
the probe tip into a clean container.
5. Dip the probe into the container, immersing the probe tip.
6. Stir the probe gently to create a uniform sample.
10

7. Press CAL to enter calibration mode. “CA”will flash on the LCD.
The pH value appears on the main display and 2.00 appears
on the secondary display (CUSTOM) or 4.00 appears on the
secondary display (N.I.S.T.).
8. When the measured pH value is stable and the Ready function
is enabled (Refer to P5.0 READY ICON page 15). “READY”will
appear on the left side of the LCD.
Note...
If the pH value continues to display different values, check the buffer or
probe. (Refer to TROUBLESHOOTING page 32).
9. N.I.S.T. buffer: Skip to step 10. CUSTOM buffer: Follow
instructions for your selected buffer solution:
pH 4
Press HLD/REC. 4.50 appears on the secondary display.
Use ▲or ▼to adjust to 4.00.
pH 7
Press HLD/REC. 4.50 appears on the secondary display.
Press HLD/REC again. If the secondary value does not default to
7.00, use ▲or ▼to adjust to 7.00.
pH 10
Press HLD/REC. 4.50 appears on the secondary display.
Press HLD/REC again. 7.00 appears on the secondary display.
Press HLD/REC again. 9.50 appears on the secondarydisplay. Use
▲or ▼to adjust to 10.00.
10. Press MN/MX/AV to confirm. Press ESC to save and return to
Normal Mode.
11. Change the buffer solution and repeat previous steps to achieve
multiple point calibration. Clean the probe in between each buffer.
11

MEASUREMENT PROCEDURES
1. The meter is powered by 4 AAA batteries. Install batteries or
connect an AC adapter to the power jack on the side of the meter.
2. Connect a sensor probe to the BNC connector on top of the meter.
3. For the pH probe with temperature sensor, also connect the plug
into the jack to the right of the BNC connector.
4. Connect the USB cable to the meter and computer to upload
measurements for computer analysis.
Available measurement parameters of each probe:
pH mV
pH Probe Yes Yes
ORP Probe N/A Yes
IMPORTANT: The temperature of the measured liquid must be stable.
pH Measurement
This meter is designed to take readings with automatic or manual
temperature compensation. Automatic temperature compensation
only occurs when a temperature sensor is plugged into the meter.
For manual temperature compensation, the default setting is 25°C.
It is also possible to manually adjust the temperature to match your
working conditions, as measured by a separate thermometer.
To take measurements:
1. Remove the pH probe soaker bottle by rotating the bottle and
cap and slide the bottle and cap oႇthe probe. Rinse the probe
tip with de-ionized or distilled water before use. If the probe tip is
dehydrated, soak it for 30 minutes in a KCl solution. DO NOT wipe
the pH probe dry. Wiping the probe may cause static and cause
calibration and measurement instability.
2. Press POWER to turn on. ATC appears to indicate that the automatic
temperature compensation probe is connected and working properly.
3. Immerse the probe tip (glass bulb) completely into the sample.
4. Stir the probe gently to create a uniform sample.
5. Wait until the reading has stabilized.
If selected in setup, “READY”
illuminates to indicate a stable reading.
12

mV Measurement (± 499mV)
mV measurement range is from -499 mV to +499 mV
with a pH probe.
1. Follow Step 1 in the pH Measurement section to clean and
soak the probe.
2. Press POWER to turn on. Press MODE to select mV mode.
3. Follow Steps 3-5 in the pH
Measurement section to
obtain a reading.
4. To switch between mV and pH,
press MODE.
ORP (mV) Measurement (± 1999mV)
ORP (Oxidation Reduction Potential) measurement range is
-1999 mV to +1999 mV. Use an ORP probe for measurement:
1. Follow Step 1 in the pH Measurement section to clean
and soak the probe.
2. Press POWER to turn on. Press
MODE to select mV measurement.
3. Follow Steps 3-5 in the pH Measurement
section to obtain a reading.
Note...
There is no need to take temperature compensation into
consideration when using an ORP probe to measure.
Temperature Compensation
Automatic with pH probe (ATC):
Plug the temperature sensor connector into the jack on top of the
meter next to the larger BNC connector.
Manual with pH probe (MTC):
1. To set the temperature, press MN/MX/AV for more than
2 seconds, “CA”will flash on the LCD Display.
2. Press the ▲or ▼to change the temperature value and
then press MN/MX/AV to save and return to Normal Mode.
13

Hold Function
This function allows you to freeze the
current reading on the display in
Normal Mode.
1. Press HLD in Normal Mode. “HLD”
appears on the display.
2. Press HLD and the meter returns to Normal Mode.
Record Memory
The meter can store up to 99 records each of pH, mV, and
ORP (mV) readings.
1. In any measurement or Hold Mode,
press MEM to save the data.
2. The memory number and measured
value will flash and then return to
Normal Mode.
Note…
Further data cannot be saved once the memory is full.
See Clear Memory on page 11 to create additional space.
Recall Memory
This function recalls readings stored in the memory.
1. Press REC for more than 2 seconds
to enter Recall Mode. “REC”will flash on the LCD display.
2. Press ▲to select next memory content. Press ▼to select
previous memory.
3. To exit memory recall, press REC for
more than 2 seconds.
14

Recall Maximum & Minimum
This function reviews a maximum and minimum value for all the
data points stored in memory.
1. Press REC for 2 seconds to enter Recall Mode. “REC”will
flash on the LCD.
2. Press MN/MX/AV to view the minimum value of the memory.
Press MN/MX/AV again to view the maximum value.
3. To exit memory recall, press REC for more than 2 seconds.
Backlight
1. Press any button to activate the backlight function.
The backlight turns oႇautomatically after 10 seconds of inactivity.
Auto Power Off
The meter turns off automatically after
20 minutes of inactivity. To override the
function, hold down POWER and HLD
simultaneously for 2 seconds when turning the
meter on until “n”appears.
Note…
The auto power off function will be disabled while in calibration mode.
15

MAINTENANCE
pH Probe
It is important to keep the pH
probe wet when the meter is
not in use. The probe is well
protected by a plastic bottle
containing solution. To use
or store the probe:
1. Rotate the bottle to remove
from the probe. Pull down
the cover and remove it
from the probe.
2. After use, put cover back on
the probe and plug the probe
into the bottle. Rotate the
bottle to fit into the cover tightly.
The following actions will keep the probe in good working condition.
•Always keep the pH glass bulb wet by using the plastic bottle
to protect and store the probe. You can also store in a KCl
solution. Never use distilled or de-ionized water for storage.
•Always rinse pH probe in de-ionized water before using.
•Never touch or rub the glass bulb tip.
Probe Testing
1. Connect the ORP probe to the meter via the BNC connector.
2. Put the probe in a buffer solution of pH 7.00 with saturated
quinhydrone.
3. Stir; mV reading (E1) should be 86 ±15mV.
4. Rinse the probe with distilled water between each use. Keep
the ORP probe wet. If not in use for long periods, it should be
rinsed and stored in the soaker bottle filled with the saturated
KCL solution.
16

ORP Probe Cleaning
A contaminated sensing element can result in a slow response
and/or inaccurate reading.
•If contamination is a mineral matter, put the sensing element in
a HCl solution 0.1 N for 10 minutes. Then rinse in distilled water.
•If contamination is oil or grease, clean with a mild detergent.
Then rinse with distilled water.
Upon completion of either cleaning method, immerse the probe in a
saturated buffer solution with pH 4.01 for 15 minutes and rinse with
distilled water.
Probe Performance
The sensingelement of an ORP probe is made of a high purity metal.
Slow response time and inaccurate readings may be caused by the
sensing element previously having been soaked in a solution for a long
period of time. An oxidation reduction coating may have formed on the
surface of the sensing element. Resolve by cleaning the element.
When measuring solutions with a low concentration of oxidation
reduction matter and slow ion exchange rate, a slow response time and
inaccurate readings may occur. Under these conditions, it may take
8-24 hours to obtain an accurate reading.
17

TROUBLESHOOTING
Meter does not turn on:
1. Press POWER for more than 2 seconds.
2. Check connections of the power adapter or batteries.
Unstable readings:
1. Stir the solution to make a uniform sample and make sure the
sensor is completely immersed in the solution. The measurement
must be done while probe is in the container/solution.
2. Move to new location for measurement, RF emissions from
unknown source may disrupt readings.
Readings not changing:
1. Check to see if meter is in “HOLD”mode.
2. Release “HOLD”function.
3. Check to see if meter is in “MTC,”if so, input temperature value.
Slow response:
1. Clean and re-calibrate the probe. Replace with a new probe.
Wrong real time:
Incorrect real time display will not affect the measurements. Internal
batteries (separate from internal batteries that power the meter)
need replacing. Contact Sper Scientific for replacement
documentation.
ERROR CODES
E02 Reading is under the lower limit
E03 Reading is over the upper limit
E04 Temperature sensor fail, replace probe.
E12 Factory calibration data error; Reset meter
E13 Slope or Offset value of pH probe is out of range
E31 Measuring circuit failure; Restart meter
E32 Memory Integrated Circuit failure
18

PC CONNECTION
The meter can interface with a personal computer to capture
on-line or stored data.
Connection procedures:
1. Plug the USB cable into the cable jack on the side of the meter.
2. Plug D-sub 9 pin type connector into a computer Serial COM port.
COM ports 1-8 can be used.
3. Insert CD-Rom in computer and follow the procedure in the
operation manual located on the CD.
Protocol information
USB protocol settings: 9600 bps, 8 data bits, no parity.
(Transmits ASCII code every second.)
Normal Data:
pxx.xxpH: mxx.xxmV:Txxx.xC(F)@ 2007-04-18
18:48:48LRCCRLF
Protocol Information
Errors:
ExxNul: ExxNul: ExxNul @ 2007-04-18
18:48:48LRCCRLF
Description:
$pH:mV:TpH LRC CRLF
Note...
The first value is pH reading in pH, the second value is
Voltage reading in mV, the third value is Temperature of pH probe in C/F,
and “x”is a numerical value.
19

Format in Memory Transmit pH Mode
Normal Data:
Pxx.xxpH: Txxx.xC(F)#xx@2007-04-18
18:48:48LRCCRLF
Errors:
ExxNul: ExxNul: #xx @2007-04-18
18:48:48LRCCRLF
Description:
$pH: Temp LRCCRLF
20