Sea-Bird Scientific SeaFET V2 User manual

User manual
SeaFET™ V2 and SeapHOx™ V2
pH and optional CTD and DO sensor
Document No. SeaFETV2SeapHOxV2
Release Date: 2020-10-05
Version: A
Software: UCI 2.03
425-643-9866
seabird.com

Table of Contents
Section 1 SeaFET V2 and SeapHOx V2 quick start guide......................................................... 3
Section 2 Specifications.................................................................................................................... 5
2.1 Mechanical................................................................................................................................... 5
2.1.1 SeapHOx V2....................................................................................................................... 5
2.1.2 Bulkhead connectors........................................................................................................... 5
2.1.2.1 SBE-37-SMP-ODO connector.................................................................................... 5
2.1.2.2 SeaFET V2 connector................................................................................................ 5
2.1.3 Illustrations.......................................................................................................................... 6
2.2 Electrical....................................................................................................................................... 8
2.2.1 SeaFET V2......................................................................................................................... 8
2.2.2 SeapHOx............................................................................................................................. 8
2.2.3 SBE-37-SMP-ODO ............................................................................................................9
2.3 Analytical...................................................................................................................................... 9
2.3.1 SeaFET V2.......................................................................................................................... 9
2.3.2 SeapHOx™ V2.................................................................................................................... 9
Section 3 Product overview............................................................................................................ 11
3.1 Ion-sensitive field effect transistor (ISFET)................................................................................ 12
3.2 LED status indicator and batteries............................................................................................. 12
Section 4 Set up sensor and verify function.............................................................................. 15
4.1 Batteries..................................................................................................................................... 16
4.1.1 Alkaline batteries............................................................................................................... 16
4.1.2 Lithium batteries................................................................................................................ 16
Section 5 Deployment and recovery.............................................................................................19
5.1 Deployment orientation.............................................................................................................. 20
5.2 Configure sensor with deployment wizard................................................................................. 20
5.3 Remove wet cap........................................................................................................................ 21
5.3.1 Attach anti-fouling guard and deployment cable............................................................... 21
5.4 Real-time data collection............................................................................................................ 21
5.4.1 Start data collection........................................................................................................... 21
5.4.2 Look at collected data....................................................................................................... 21
5.4.3 Save real-time data........................................................................................................... 22
5.4.4 Configure data file headers............................................................................................... 22
5.5 Recover sensor from deployment.............................................................................................. 23
Section 6 Transmit and process data...........................................................................................25
6.1 SeaFET V2 output formats......................................................................................................... 25
6.2 SeapHOx V2 output formats...................................................................................................... 26
6.3 Process data with temperature and salinity corrections............................................................. 26
6.4 Export data to .csv or MS Excel format ..................................................................................... 27
6.5 Show data from the sensor........................................................................................................ 28
6.6 Erase data stored in sensor....................................................................................................... 28
Section 7 Maintenance..................................................................................................................... 29
7.1 Remove or attach wet cap......................................................................................................... 29
7.2 Clean electrode surfaces........................................................................................................... 30
7.3 Clean flow paths......................................................................................................................... 30
7.4 Remove or attach mounting hardware....................................................................................... 31
7.5 Remove and replace alkaline batteries...................................................................................... 32
7.6 Remove or replace anti-fouling devices..................................................................................... 35
7.7 Examine O-rings........................................................................................................................ 36
7.8 Clean bulkhead connectors........................................................................................................ 37
7.9 Prepare sensor for storage........................................................................................................ 38
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7.9.1 CTD storage...................................................................................................................... 38
7.9.2 pH sensor storage............................................................................................................. 38
7.10 Calibration................................................................................................................................ 38
7.10.1 Manufacturer pH calibration............................................................................................ 38
7.10.2 Verify calibration.............................................................................................................. 39
Section 8 Reference..........................................................................................................................41
8.1 Terminal program setup and use............................................................................................... 41
8.2 Terminal commands................................................................................................................... 41
8.3 Polled data collection................................................................................................................. 43
Section 9 Software reference......................................................................................................... 45
9.1 Sensor dashboards.................................................................................................................... 45
9.2 UCI menu................................................................................................................................... 45
9.2.1 General tab........................................................................................................................45
9.2.2 Display tab.........................................................................................................................46
9.2.3 Message tab...................................................................................................................... 47
9.3 Sensor menu.............................................................................................................................. 47
9.3.1 SeaFET, SeapHOx............................................................................................................47
9.4 Data menu.................................................................................................................................. 47
Section 10 General information..................................................................................................... 49
10.1 Warranty................................................................................................................................... 49
10.2 AF24173 anti-foulant device.................................................................................................... 49
10.3 Service and support................................................................................................................. 51
10.4 Waste electrical and electronic equipment............................................................................... 52
Table of Contents
2

Section 1 SeaFET V2 and SeapHOx V2 quick start guide
This quick start guide gives the steps necessary to make sure that the SeaFET V2 or
SeapHOx V2 sensor operates correctly and collects data before it is deployed.
• SeaFET V2 measures pH only
• SeapHOx V2 is a SeaFET with a SBE-37-SMP-ODO attached. It measures the
added parameters of conductivity, temperature, pressure, and dissolved oxygen. The
two sensors are integrated by the manufacturer.
The SeaFET V2 acts as a controller for the SBE-37, which sends the data it collects to
the SeaFET V2. Because the SBE-37 pump operates before each burst of data
collection, the manufacturer recommends that the shortest data collection interval is every
two minutes for the SeapHOx. The shortest data collection interval for a SeaFET is
6 seconds. Use the battery endurance calculator in the software to determine the length
of a deployment.
NOTICE
Do not let the sensing elements get dry. If the sensor will not be deployed immediately, put the wet
cap in place and fill with clean seawater.
What's in the box:
SeaFET V2
• Flow cells and fittings
• Anti-fouling guard
• CD—has software, calibration files, documentation
• RS232 cable to connect to a PC
• SeaFET V2 toolkit and O-rings
• Hardware kit for boss seal
SeapHOx V2
• Flow cells and fittings
• Anti-fouling guard
• CD—has software, calibration files, documentation
• Y-cable to connect the SeaFET V2 and the SBE-37-
SMP-ODO
• RS232 cable to connect the Y-cable to a PC
• 2.4 m cable to communicate directly with the SBE-37-
SMP-ODO only
• SeaFET V2 toolkit and O-rings
• Mounting brackets (2) to attach the SBE-37 to the
SeaFET V2
• Plumbing kit and Triton® X-100 to clean MicroCAT flow
path.
• Hardware kit for boss seal
The SeaFET V2 has 12 size D alkaline batteries installed. The 12 lithium batteries for the
SBE-37 are shipped separately.
1. SeapHOx V2: Install the manufacturer-supplied lithium batteries in the SBE-37 (refer
to Lithium batteries on page 16 for details.)
a. Remove the end flange of the SBE-37.
b. Disconnect the battery holder and remove it from the sensor.
c. Install new batteries.
d. Connect the battery pack to the sensor again and install the end flange again.
2. SeapHOx V2: Remove the yellow protective label from the SBE-37 intake and
exhaust ports.
3. SeapHOx V2:
a. Connect the SeaFET V2 and SBE-37 with the manufacturer-supplied Y-cable.
b. Connect the Y-cable to the manufacturer-supplied data I/O cable.
4. SeaFET V2:
• Connect the sensor to the manufacturer-supplied data I/O cable.
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5. Install the manufacturer-supplied software on a PC (refer to Install and start software
for details.)
6. Connect the data I/O cable to the PC and double-click on UCI to start the software.
7. Push Connect in the software to turn on the sensor.
8. Set up the sensor for deployment (refer to Configure sensor with deployment wizard
on page 20 for details.)
9. Verify that the boss seal on the SeaFET V2 is screwed in all the way.
The sensor will flood if the plug is not screwed in all the way.
10. Immediately before the SeapHOx V2 is deployed, connect the plumbing to the
SBE-37.
a. Use a 5/32" hex key to remove the three screws that attach the wet cap to the
pressure housing.
The clean seawater in the wet cap will drain.
b. Remove the two threaded plastic plugs and install the manufacturer-supplied flow
cells with the wet cap (refer to Remove or attach wet cap on page 29 for
details.)
c. Install the flow cell again. Make sure the fitting is securely connected to the
exhaust port on the MicroCAT.
d. Secure the two plastic plugs into the wet cap again.
11. Connect a dummy plug to the Y-cable for deployment.
NOTICE
Make sure that the screw-in boss seal is tightened or the sensor will flood and void the
warranty.
12. Transmit and process data that is stored in the sensor to the PC (refer to Transmit
and process data on page 25for details):
a. Connect the sensor to the UCI software and PC and start the software.
b. Push Transmit Data in the Dashboard of the software.
c. Select "All Data" or "Sample Number Range" in the Data Transmit Options area.
d. Select either "UTC" or "Local" time stamp in the CSV Format Options area.
e. Enter a new file name or use the automatically generated file name.
f. Push Transmit.
The software copies the data to the PC in both a raw .sbsdat format and a
converted .csv format.
13. Immediately after the SeaFET V2 or SeapHOx V2 is recovered from a deployment
(refer to Recover sensor from deployment on page 23 for details):
a. Use the software to turn off the sensor.
b. Disconnect the fittings from the flow cell, fill the flow cell with clean seawater and
secure the two red plugs to keep fresh water out of the flow cell.
c. Rinse the sensor with fresh water.
d. SeapHOX: Disconnect the Y-cable and remove the SBE-37 from the mounting
bracket.
e. SeapHOx: Clean the flow path of the SBE-37.
14. Dry and lubricate the bulkhead connectors. Refer to Clean bulkhead connectors
on page 37 for details.
15. Attach the protective dummy plugs and lock collars.
16. Refer to Prepare sensor for storage on page 38 for details to prepare the sensor for
short- or long-term storage.
SeaFET V2 and SeapHOx V2 quick start guide
4

Section 2 Specifications
2.1 Mechanical
2.1.1 SeapHOx V2
SeaFET 37-SMP-ODO
Rated depth 50 m 20, 100 m
Weight 5.45 kg 3.4 kg (air)
Length 55.88 x 28.25 cm 55.5 cm
Diameter 11.4 cm N/A
Temperature range, operation 0–50 °C -5–45 °C
Temperature range, storage 2–55 °C —
2.1.2 Bulkhead connectors
2.1.2.1 SBE-37-SMP-ODO connector
Contact Function MCBH-4-MP
1 Ground
2 RS232 RX
3 RS232 TX
4 Voltage in
2.1.2.2 SeaFET V2 connector
Contact Function MCBH-8-MP
1 Voltage in
2 Ground
3 No connect
4 CTD/pump V out (12 V, 650 mA, optional)
5 TXD/D+
6 RXD/D-
7 CTD TXD
8 CTD RXD
5

2.1.3 Illustrations
Figure 1 SeaFET V2 dimensions
Specifications
6

Figure 2 "Y" cable for SeapHOx V2
Figure 3 Data I/O cable
Specifications
7

Figure 4 SBE-37-SMP-ODO-only test cable
2.2 Electrical
2.2.1 SeaFET V2
Input 6–18 VDC
Internal batteries 12 D-cell 1.5 V alkaline
Main battery pack 12 V, 13420 mA-hours
Isolated battery 6 V
Real-time clock drift 2 ppm (0–40 °C)
Communication interface RS232: 9600–115200 baud; Default: 19200 baud
Data storage 32 Mb (over 1240000 samples)
Current draw, operation
CTD OFF, data transmit OFF 17.3 mA (2.5 sec sample time)
CTD OFF, data transmit ON 17.0 mA (2.7 sec sample time)
Current draw, idle 3.4–4.0 mA
Current draw, quiescent Main battery: 70 µA; Isolated battery: 200 µA @ 12 V
2.2.2 SeapHOx
Input 6–18 VDC
Internal batteries 12 D-cell 1.5 V alkaline
Main battery pack 12 V, 13420 mA-hours
Isolated battery 6 V
Current draw, operation
CTD OFF, data transmit OFF 18.9 mA (2.5 sec sample time)
CTD OFF, data transmit ON 21.1 mA (2.6 sec sample time)
CTD ON, data transmit OFF 21.7 mA (7.2 sec sample time)
CTD ON, data transmit ON 23.1 mA (7.2 sec sample time)
Specifications
8

Current draw, pump 10 mA
Current draw, idle 3.4–4.0 mA
Current draw, quiescent Main battery: 70 µA; Isolated battery: 200 µA @ 12 V
Real-time clock drift 2 ppm (0–40 °C)
Communication interface RS232: 9600–115200 baud Default: 19200 baud
Data storage 32 Mb (over 1240000 samples)
2.2.3 SBE-37-SMP-ODO
Input 9–24 VDC
Internal batteries 12 AA-cell 3.6 or 3.9 V lithium
Current draw, operation 0.29 watts
Current draw, low power 30 µA
Current draw, communication 4.3 mA
2.3 Analytical
2.3.1 SeaFET V2
Measurement range 6.5–9.0 pH
Salinity range 20–40 psu
Accuracy ±0.05 pH
Precision 0.004 pH
Resolution 0.0001 pH
Stability 0.003 pH/mo
2.3.2 SeapHOx™ V2
Conductivity Temperature Depth Oxygen
Measurement
range
0–70 mS/cm -5–45 °C 20, 100 m 120% surface saturation
Accuracy ±0.003 mS/cm ±0.002 °C (-5–35 °C);
±0.01 °C (35–45 °C)
±0.1% full scale
range
±0.1 mg/L or ±2%
Resolution 0.0001 mS/cm 0.0001 °C 0.002% full scale
range
0.2 µmol/kg
Stability 0.003 mS/cm/mo 0.0002 °C/mo 0.05% full scale
range/yr
sample-based drift
<1 µmol/kg/100,000 samples @20 °C
Specifications
9

Specifications
10

Section 3 Product overview
NOTICE
When sensor is not in use, make sure that the wet cap is in place and is filled with clean seawater.
Do not put the sensing elements in fresh water: it may cause data to be unstable and damage to
the sensor.
NOTICE
Do not let the potassium chloride (KCl) gel or the wet cap filling solution freeze. This will damage
the DuraFET and void the warranty.
The sensor uses ion-sensitive field effect transistor (ISFET) technology to measure pH in
marine environments at depths to 50 meters. The sensor has internal memory and an
internal battery pack so that it can operate autonomously for a long-term deployment.
When an SBE-37-SMP-ODO is attached to the SeaFET, the system operates in
SeapHOx mode, which also measures salinity, temperature, depth, and oxygen, and has
an internal pump. The SeapHOx uses a manufacturer-attached "Y" cable to connect the
SeaFET and SBE-37-SMP-ODO.
The manufacturer-supplied software lets the user set up the sensor, monitor graphical
data in real time, upload stored data, and process that data.
The end flange with the ISFET sensor and the external reference electrode must be
covered with a seawater-filled cap during storage. This cap is also used with flow cells
when the SeaFET is connected to the SBE-37-SMP-ODO.
11

3.1 Ion-sensitive field effect transistor (ISFET)
The primary sensor element of the SeaFET™ is the ISFET, a solid-state sensor that
senses pH in marine environments. The ISFET has two reference electrodes: an internal
reference and an external reference, that give separate reference potentials to the ISFET
and show separate pH values (pH Internal and pH External). After the corrections for
temperature and salinity are applied, the values from the internal and external are similar,
and let the user verify the validity of the sensor's measurements.
Internal reference
The internal reference electrode inside the DuraFET® is immersed in a bath of saturated
potassium chloride (KCl) gel and is physically separated frm the environment. The KCl
gel exposes the Ag/AgCl internal electrode to a relatively constant chloride concentration.
The sensor can therefore measure pH regardless of environmental salinity. If accurate
salinity and temperature data are not available, the internal cell is generally more
accurate.
External reference
The external reference electrode has a Ag/AgCl reference electrode in direct contact with
seawater. The potential of this electrode varies with pH and chloride concentration, so
unless the chloride concentration is known, the external reference is not stable. To
correct this, salinity can act as an approximation of chloride concentration. If accurate
salinity data is available, it can be applied to the pH external data and significantly reduce
measurement errors, and give the most accurate and stable pH data.
3.2 LED status indicator and batteries
The LED near the magnetic power switch shows the status of the sensor, so you can
determine if the sensor is in operation or ready to be deployed. To see the status of the
sensor, swipe the manufacturer-supplied magnet across the red circle at the top of the
magnetic switch. If the LED does not flash, verify that new batteries are installed and the
memory is configured. None of the settings on the SeaFET are affected when you use the
magnet to look at the status.
Product overview
12

Table 1 LED status flashes
No flash Sensor batteries or
memory not ready for
deployment
• RTC battery is below 2.5V
• Isolated battery is below 4.0V
• Main battery or external power supply is below 7.0V
• Memory is full
Red flash Sensor has not received
a command to collect
data
• The sensor is in standby for a command to begin data collection
• Push Start to start data collection. ("startnow" or "startlater" in a terminal
program.)
Green
flash
Sensor is in operation Logger-controlled (polled) data collection occurs. The sensor has received a "Start,"
"startnow," or "startlater" command.
The internal batteries must be installed in the SeaFET at all times for the preservation of
the sensing element. If the internal batteries are removed, the sensor must be "re-
conditioned" before or at a deployment, which can take up to 24 hours.
SeaFET V2 batteries
There are 12 manufacturer-installed D-cell batteries to supply power to the sensor.
The internal batteries are electrically divided into a 12 V main battery pack and a 6 V
isolated battery pack. The main battery pack supplies power to the sensor and the
isolated battery pack supplies power to the sensing element when the sensor is in a low
power "standby" mode.
SeapHOx: SBE-37-SMP-ODO integrated with SeaFET V2
SBE-37-SMP-ODO batteries
The SBE-37-SMP-ODO attached to the SeaFET uses lithium batteries that ship
separately and must be installed before use if the SeaFET is not set up to supply power
to the SBE-37-SMP-ODO.
Power options during deployment
Power can be supplied to the SeapHOx from either the SeaFET internal batteries or an
external power source. The sensor will use an external source when the voltage is at or
above 9 volts. The user can configure the SeaFET to supply power to the SBE-37-SMP-
ODO. For maximum lifetime of the SeaFET batteries, install new lithium batteries in the
SBE-37-SMP-ODO and configure the SeapHOx without a power supply to the SBE-37-
SMP-ODO.
Product overview
13

Product overview
14

Section 4 Set up sensor and verify function
NOTICE
Make sure that the SeapHOx is submerged in water before operation to prevent damage to the
pump. The pump always operates when the SeapHOx takes a measurement. Before a
deployment, set the start time to occur after the sensor is submerged. The SeapHOx does not use
a minimum conductivity value for the pump to operate.
Do the steps below to make sure the sensor operates correctly before further setup and
deployment. Use the manufacturer-supplied UCI software to set up and configure the
sensor and to transmit data.
1. Connect the sensor and the PC.
• SeaFET standalone: connect the data I/O cable to the PC.
• SeapHOx: Make sure that the Y-cable is installed and connects the SeaFET and
the SBE-37-SMP-ODO. Connect the I/O cable to the Y-cable and the PC.
2. Get the software from the manufacturer's website or the manufacturer-supplied USB
drive or CD.
3. Install the appropriate software.
a. For Windows®: Double-click on the file with ".exe" appended to the name.
b. For Mac OS X®: Double-click on the file with ".pkg" appended to the name. Make
sure that the default "Install for all users on this computer" is selected as the
destination for the installed software. If "Install for me only" or "Install on a
specific disk" is selected, the software will not connect to the sensor.
4. Push Run in the new window.
The setup wizard starts.
5. Follow the on-screen instructions to install the software.
6. Push Connect in the Dashboard area.
7. If necessary, change the "Instrument Type" to the connected sensor.
8. Put a check in the "Try All Baud Rates" box.
The software automatically finds the correct baud rate.
9. Select the UCI menu, then Preferences.
10. Go to the General tab and push Browse to find or make the Default Data Directory
on the PC.
Data from the sensor is saved here.
11. Optional: save data directly to the PC.
a. Go to the View menu and select Data Collection.
b. Push "Collection Options" to see details in this tab.
c. Push Browse to change the directory to which data is saved on the PC.
d. Push Start.
12. Push OK.
13. Push Apply to store the settings in the sensor.
14. Push Start in the Dashboard area.
The sensor operates and collects data.
15. Look at the data in the Time Series graph. The user can look at data in real-time for
each sensor that has power supplied, is connected and is in communication with the
software.
• Put a check in the box next to "Time Axis" to push Zoom In and Zoom Out to
change the scale of time.
• Put a check in the box next to "Range Axis" to push Zoom In and Zoom Out to
change the scale of the data.
15

• To move the data in any direction, push the "Ctrl" key on the PC keyboard and
the left button of the mouse pointer at the same time.
• To select a specific part of the data to zoom in on, pull the mouse pointer
diagonally.
• Push Auto Range to see the data for each selected parameter. The software
adjusts the scale so that the data will always show.
• Push Default Ranges to go back to the manufacturer-set default scale for each
parameter.
• Put a check in the box next to "Show Data Points" to see the value of the
collected data when the mouse moves over each point.
• Push Select Sensors To Display to change the parameters to look at in the
Time Series graph.
16. Push Stop. The "Connection Mode" shows "Setup."
4.1 Batteries
4.1.1 Alkaline batteries
Twelve D-cell alkaline batteries are installed in the SeaFET by the manufacturer. The
user should verify that the batteries are installed correctly and supply the specified
voltage. Refer to Remove and replace alkaline batteries on page 32 for details about
how to change the batteries when necessary.
4.1.2 Lithium batteries
NOTICE
Use only the batteries recommended by the manufacturer as replacements. Do not mix
chemistries, V-Ah, or new and used batteries.
The manufacturer ships the twelve lithium batteries for the SBE-37-SMP-ODO separately.
Do the steps below to install or replace the batteries. See also the "how to" video on the
manufacturer's website to do this procedure.
Table 2 Recommended lithium battery brands
SAFT LS-14500 (included) 3.6 V, 2.6 Ah
Tadiran TL-4903 3.6 V, 2.4 Ah
Electrochem BCX85 series 3.9 V, 2.0 Ah
1. Make sure that the end flange and pressure housing are dry.
2. Use a 9/64" hex key to remove the two screws on the sides of the pressure housing.
3. Install these two screws into the sides of the end flange to start to loosen the end
flange.
4. Turn the end flange counter-clockwise to loosen it from the pressure housing.
5. Pull gently to disconnect the battery wires in the end flange from the battery pack.
6. Use a lint-free tissue to remove any water from the O-ring surfaces inside the
pressure housing and end flange.
Set up sensor and verify function
16

7. Use a 7/64" hex key to loosen the captured screw in the battery cover plate.
8. Remove the battery pack from the pressure housing.
9. Turn the yellow cover plate counterclockwise to remove it from the battery pack body.
10. Move each of the two O-rings on the outside of the battery holder from the grooves.
It makes it easier to remove or insert batteries.
11. Examine the O-rings and surfaces for dirt, cuts, or other damage.
Clean or replace as necessary.
12. If necessary, remove the batteries in the pack.
13. Insert new batteries.
Make sure to alternate the positive (+) and the negative (-) ends on the batteries to
agree with the labels on the pack as they are installed.
14. Move the O-rings back into the grooves.
15. Align the pin on the yellow battery cover with the post hole in the battery pack
assembly.
16. Align the "D"-shaped part of the battery pack with the pins on the shaft.
17. Slowly move the assembly onto the housing. Push gently to connect the battery
assembly with the circuit board in the pressure housing.
18. Use a 7/64" hex key to tighten the captured screw on the yellow battery cover onto
the shaft in the pressure housing.
19. Important! Remember to attach the Molex connector on the end flange to the
connector on the top of the guide pylon in the pressure housing.
Set up sensor and verify function
17

20. Examine the O-rings on the end flange. They must be pristine, with no lint or
scratches or chips.
• Apply a small quantity of Parker Super O Lube® to any new O-rings.
21. Align the end flange holes with the holes in the pressure housing.
22. Carefully push the end flange into the pressure housing.
It may help to rotate the end flange so that the wires do not bend too much.
23. If necessary, use a 9/64" hex key to remove the two screws from the end flange.
24. Use a 9/64" hex key to install the two screws into the pressure housing of the sensor
again.
Set up sensor and verify function
18