Shel lab BACTRON300 User manual

BACTRON® 110 –120 Volts
Installation and
Operation Manual
BACTRON300, BACTRON600, BACTRON900
Previously Designated:
BACTRONII, BACTRONIV, BACTRONIV-900

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BACTRON Anaerobic Workstation 110 - 120 Volts
Installation and Operation Manual
Part number (Manual): 4861700-1
Revision: January 20, 2016
Pictured on Cover: BACTRON900 (BACTRONIV-900)
BACTRON600 (BACTRONIV)
BACTRON300 (BACTRONII)
These units are TÜV CUE listed as anaerobic chambers for professional, industrial, or educational
use where the preparation or testing of materials is done at an ambient air pressure range of 22.14
–31.3 inHg (75 –106 kPa) and no flammable, volatile, or combustible materials are being heated.
These units have been tested to the following requirements:
CAN/CSA C22.2 No. 61010-1:2012
CAN/CSA C22.2 No. 61010-2-010:2004 Reaffirmed: 2014-07
UL 61010-1:2012-05
UL 61010A-2-010:2002-03
EN 61010-1:2010
EN 61010-2-010:2014
Supplemented by: UL 61010-2-010:2015

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TABLE OF CONTENTS
INTRODUCTION........................................................................................................................................... 6
General Safety Considerations.................................................................................................................6
Engineering Improvements .......................................................................................................................7
Contacting Assistance...............................................................................................................................7
RECEIVING YOUR BACTRON....................................................................................................................8
Inspecting the Shipment............................................................................................................................8
Orientation Photos.....................................................................................................................................9
Record Data Plate Information................................................................................................................12
Temperature Reference Sensor Device .................................................................................................12
Catalyst Oven..........................................................................................................................................12
INSTALLATION..........................................................................................................................................14
Installation Checklist ...............................................................................................................................14
Required Ambient Conditions .................................................................................................................15
Workspace ..............................................................................................................................................15
Environmental Disruption Sources..........................................................................................................15
UV Lighting..............................................................................................................................................15
Power Source Requirements..................................................................................................................16
High Altitude Effects................................................................................................................................16
Lifting and Handling ................................................................................................................................17
Leveling...................................................................................................................................................17
Install the BACTRON ..............................................................................................................................17
Gas Supply Requirements ......................................................................................................................18
Connect the Gas Supply .........................................................................................................................19
Connect to a Vacuum Supply Source.....................................................................................................20
Connect the Foot Pedal Controls............................................................................................................21
Fill the Manometer...................................................................................................................................22
Installation Cleaning and Disinfection.....................................................................................................22
Place Anaerobic Monitoring Strips..........................................................................................................23
Install Shelf Spacers ...............................................................................................................................23
Prepare the Incubators............................................................................................................................23
Install the Arm Port Doors.......................................................................................................................24
GRAPHIC SYMBOLS.................................................................................................................................26
CONTROL PANEL OVERVIEW.................................................................................................................29
OPERATION...............................................................................................................................................33
Theory of Operation and Major Component Functions...........................................................................33
Place the BACTRON in Operation..........................................................................................................36
Establish an Anaerobic Atmosphere.......................................................................................................37
Attach the Sleeve Assemblies.................................................................................................................41
Chamber Entry........................................................................................................................................42
Moving in the Pressurized Chamber.......................................................................................................43
Anaerobic Monitoring Strips....................................................................................................................43
Verifying an Anaerobic Atmosphere........................................................................................................44
Troubleshooting Oxygen in the Chamber ...............................................................................................45
Exiting the Chamber................................................................................................................................46
Set the Temperature Set Point................................................................................................................47
Temperature Accuracy Verification.........................................................................................................48
Set the Over Temperature Limit..............................................................................................................50
Cycling the Pass Box ..............................................................................................................................51
Load Samples .........................................................................................................................................53
Reactivating the Catalyst Cartridges.......................................................................................................54
AMG Conservation Methods...................................................................................................................54

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Volatile Compound Scrubber and Rejuvenation Cycle...........................................................................55
Attaching Equipment to the Chamber Accessory Outlets.......................................................................55
Deionized and Distilled Water.................................................................................................................56
Humidifying Incubators............................................................................................................................56
Pressure Unit Conversion .......................................................................................................................56
USER MAINTENANCE...............................................................................................................................58
Daily Maintenance...................................................................................................................................59
Normal Gas Consumption.......................................................................................................................59
Leak Diagnostic –Unit in Use.................................................................................................................60
Leak Check –Empty Unit........................................................................................................................62
Door Gasket Maintenance and Usage....................................................................................................63
Sleeve Maintenance and Usage .............................................................................................................63
Replacing Sleeve Assembly Components..............................................................................................64
Cleaning and Disinfecting .......................................................................................................................66
Maintaining the Acrylic Glass Panels......................................................................................................68
Vacuum Pump Maintenance...................................................................................................................68
Electrical Components ............................................................................................................................68
Condensation and the Dew Point............................................................................................................69
Calibrate the Temperature display..........................................................................................................70
UNIT SPECIFICATIONS.............................................................................................................................74
Weight .....................................................................................................................................................74
Workstation Dimensions .........................................................................................................................74
Stand Dimensions...................................................................................................................................75
Pass Box Interior Dimensions.................................................................................................................75
Pass Box Plate Capacity.........................................................................................................................75
Incubator Dimensions .............................................................................................................................75
Capacity ..................................................................................................................................................76
Total Plate Capacity................................................................................................................................77
Temperature............................................................................................................................................77
Power ......................................................................................................................................................77
PARTS LIST ...............................................................................................................................................78
Ordering Parts and Consumables...........................................................................................................80
ACCESSORIES..........................................................................................................................................81