VWR 89404-882 User manual

Man.V100 (15 Nov. 2012)
VWR Standard Biological Microscope
Operation Manual
(Version 1.0.0)
VWR Standard Biological Microscope
89404-882, 89404-884
VWR Stand Monocular Microscope

VWR International, LLC., Standard Biological Microscope
1. SAFETY INSTRUCTIONS
1.1 General safety instructions
Please be sure to read these instructions before using the biological microscope.
Additional information is available upon request from our maintenance department or authorized
agency.
To ensure safe operation and guarantee good performance of the microscope please pay attention to
the precautions and warnings specified in the Operation Instructions.
In this Operation Instructions manual, the following symbols indicate:
Caution! Electric shock hazard!
Caution! Danger!
1.2 Instrument safety
The VWR biological microscope has been designed, manufactured and inspected according to the EN
61010-1:2001 Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory
Use.
1.3 Unpacking, transportation & storage
The original shipping container, a foam box in a fiberboard carton, should be kept for use in long term
storage or return shipment.
When unpacking, please check the components according to the packing list.
Please comply with the temperature requirements for transport and storage specified in the appendix of
this manual.
Set up, use and store the unpacked microscope on a firm and flat workbench.
Please do not touch the optical lens surfaces.
1.4 Waste disposal
Important: Any damaged biological microscope must not be treated as general waste; it should be
disposed of according to relevant regulations.
1.5 Operation
When using the biological microscope, please pay attention to the following safety instructions:
If it is used for any purpose other than the specified ones, including any individual component or part,
the manufacturer will not take any responsibility.
After-sales service or repair done by unauthorized personnel will void the warranty.
Anyone who uses the instrument should receive instruction on the proper handling of the instrument

VWR International, LLC., Standard Biological Microscope
and safety practices for microscopy. The biological microscope shall be placed only on a firm, flat
workbench for operation.
Since the biological microscope is a precision instrument, improper operation will impair or spoil its
performance.
The power unit is integrated in the main unit of the biological microscope: the grid supply voltage is
within 100-240V~60Hz.
The biological microscope must be connected only to the normal power socket with a grounding
terminal. Any extension cord without ground protection is not allowed to avoid failure of the
protection function.
If there is any electrical failure (of the fuse system, ground protection or transformer), turn off
and unplug the unit immediately. Make sure the microscope is set aside so it will not be used
again and contact the VWR service department or a VWR microscope repair agency to have it
repaired.
Please be sure to turn off the power before opening the instrument to replace LED illuminator or
replace the fuse! Only use a fuse for the rated current.
Safety instructions for the use of immersion oil.
- Immersion oil is irritating to skin; avoid contact with skin, eyes and clothing.
- Skin contact: wash with soap and plenty of water until the immersion oil is completely
removed.
- Eye contact: flush immediately with plenty of water for at least 5 minutes. If irritation persists,
seek medical advice.
- Dispose of immersion oil properly. Do not discharge into surface water or sewage.
The biological microscope is not equipped with any special device to protect against corrosive, latent
infective, toxic, radioactive or other hazardous samples. Therefore, when examining any such sample
you must comply with the relevant laws and regulations, in particular the provisions related to accident
prevention.

VWR International, LLC., Standard Biological Microscope
1.6 Quality Warranty
The VWR biological microscope and the attached accessories are only allowed to be used for
microscope examination as described in this manual. The manufacturer takes no responsibility for any
other use.
The manufacturer guarantees that the product is free from any defect in material or workmanship on
the date of delivery.
If any defect is found, notify the manufacturer immediately.
Upon receipt of the Notification of Defect as described above, the manufacture is responsible to
solve the problem either by repairing the defective instrument or replacing it with a new instrument
of the same model.
The manufacturer provides no warranty for any failure or defect due to normal wear and tear or
improper use of the product.
The manufacturer takes no responsibility for any damage caused by operation error, negligence or
unauthorized dismantling of the instrument, or the use of spare parts from other manufacturers.

VWR International, LLC., Standard Biological Microscope
2. INSTRUMENT DESCRIPTION
2.1 Part names
1. Eyepiece
7. Focusable 1.25 abbe condenser
2. Monocular head
8. Collector
3. Lock screw of eyepiece
9. Base (w/ rechargeable batteries and external charger)
4. Nosepiece
10. Head lock screw
5. 4X/10X/40X/100x objectives
11. Arm
6. Built in mechanical stage
12. Coaxial coarse & fine focusing

VWR International, LLC., Standard Biological Microscope
13. Quadruple nosepiece
18. Stage adjustment knob (Y-axis)
14. 4X/10X/40X/100X objectives
19. Base (w/ rechargeable batteries and external charger)
15. Mechanical stage
20. Binocular head
16. 1.25 condenser w/ iris diaphragm
21. Coaxial coarse and fine focus knob
17. Stage adjustment knob (X-axis)

VWR International, LLC., Standard Biological Microscope
2.2 Application
The VWR biological microscope is designed for microscopic observation of thin specimens with
transmitted, visible light.
2.3 Instrument and its major features
Major features of the instrument include:
- Built-in LED illumination with brightness adjustment.
- Cord reel at the back to accommodate power cable; convenient and
practical.
- Compact and easy to use.
- Coaxial coarse and fine focus adjustment.
- 120mm x 120mm mechanical stage with slide clips, stroke:
76mm x 26mm
- Quadruple revolving nosepiece with ball bearing, thread pitch 0.8”.
- Objective magnifications: 4X, 10X, 40X, 100X-Oil
- Field number of 10X eyepiece is 18; high point design for observers with glasses.

VWR International, LLC., Standard Biological Microscope
3. FIRST TIME USE & OPERATION
3.1 First time use
Before installing and using the biological microscope, make sure to read carefully the Safety
Instructions (See Chapter 1).
When unpacking and handling, please do not touch the optical surfaces.
After unpacking, place the biological microscope on a flat workbench and remove any foam padding
or spacer used to prevent vibration during transportation.
Connect the charger to the power supply. Before plugging in, keep in mind that the working voltage
of the biological microscope shall be the same as the supply voltage.
Turn on the power switch at the back of the arm.
Note: Make sure that the brightness control is in the minimum position before turning on or off the
power switch.
Rotate the brightness control to the desired illumination.
After use, turn the brightness control to the minimum position, and then turn off the power and put
on the dustproof cover.
3.2 Operation of the biological microscope
Put the specimen on the stage and fix it with the slide clips. Never examine a prepared specimen
without a cover slip. Note: The thickness of the cover slip must be 0.17 mm.
The aperture diaphragm of the condenser should be opened to the position matching the numerical
aperture of the objective.
For models with a movable condenser, lower or raise the condenser to locate the best illumination
for the field. Start with the condenser all the way to the top of its range.
Rotate the brightness control to the desired illumination.
Always begin examination using the low power objective. Position the objective as close to the
specimen as possible and focus by slowly increasing the distance between the specimen and the
objective. Use the coarse focus adjustment, and then the fine adjustment.
Once the specimen is in clear view under low power, switch to a higher power objective and refocus
using the FINE FOCUS KNOB ONLY. The microscope has been set up at the factory so only a
small adjustment is necessary when changing objective lenses.
Always switch back to the low power objective to change the specimen on the stage.

VWR International, LLC., Standard Biological Microscope
3.3 Modification of biological microscope
Unplug the biological microscope before making any modifications.
3.3.1 Replace the head
Unscrew the head lock screw and lift out the existing body tube.
Insert the new body tube vertically into pilot hole of the main unit, resting it on the shoulder.
Tighten the head lock screw.
3.3.2 Replace the objective
Lower the stage completely with the coarse focus knob.
Rotate the nosepiece to move the objective to be replaced to the side.
Unscrew the objective and remove it downward.
Fix the new objective into the hole on the nosepiece. Be very careful to match the threads correctly,
the objective should screw in smoothly and easily. Make sure the objective is screwed in tightly.
If one of the nosepiece holes is not used for an objective a dustproof cap should be screwed into the
vacancy to prevent dust from entering.
4. MAINTENANCE & TROUBLESHOOTING
4.1 Maintenance
The biological microscope is limited to the following maintenance only:
Turn off the power switch after use, and put on the dustproof cover after the microscope has cooled
down.
Do not operate the microscope in a room with humidity higher than 75%.
Remove dust or ordinary dirt on optical lens surfaces with a brush, rubber suction bulb and a
moistened lens tissue.
Use only optical lens tissues and optical lens cleaner (see below). Never clean a lens with a dry
optical lens tissue. Be sure to remove any dust before using lens tissue and cleaner.
To remove stubborn oily or lipoid dirt (such as immersion oil or fingerprints), dip the lens tissue into
a 3 to 7 ethanol-ether mixture or a commercially available optical lens cleaning solution and then
use it to wipe off the dirt.
When cleaning an optical lens surface, wipe gently in a circle from the center to the edge.

VWR International, LLC., Standard Biological Microscope
4.2 Troubleshooting
Problem
Cause
Remedy
Can not see the whole field
Nosepiece is not locked into the
slot
Rotate the nosepiece to lock
into the slot
Condenser is not set properly
Set the condenser properly
Aperture (iris) diaphragm is not
set accurately
Set the aperture (iris)
diaphragm accurately
Low resolution
Poor image contrast
Incorrect opening of aperture
diaphragm
Set the opening of aperture
diaphragm accurately
Improper focusing of condenser
Focus the condenser properly
Wrong thickness of cover slip
for 0.17 transmitted-light
objective
Use the standard 0.17 thick
cover slip
No immersion oil or non-
specified immersion oil for
100X/(oil immersion) objective
Use immersion oil supplied with
the instrument or go to buy
cedar oil for microscope when
supplied immersion oil used up
Bubbles in the immersion oil
Add some immersion oil or
rotate the nosepiece back and
forth to remove bubbles
Immersion oil or stain left on the
front lens of dry objective
Dirt or dust on the optical
surface of objective, eyepiece,
condenser or color filter
Clean the front lens of dry
objective (see above)
Clean the dirty optical
component
Poor LED illumination
Power plug is not plugged into
the socket properly
Insert the power plug into the
socket and turn on the power
LED illuminator damaged
Replace LED illuminator

VWR International, LLC., Standard Biological Microscope
5. APPENDIX
5.1 Technical Parameters
Dimension (W ×L ×H)
Biological microscope main unit w/ monocular head
≈ 269 × 150 × 353 mm
Biological microscope main unit w/ dual viewing head
≈ 290 × 150 × 392 mm
Weight
VWR Standard biological microscope
4.5 KG
Environmental Conditions
Transport (within package) :
Permissible environment temperature
-40 ~ +70°C
Storage:
Permissible environment temperature
Permissible relative humidity
+10 ~ +40°C
Below 31°C, max. humidity is 80%; at 40℃,
linearly decreases to 50%
Operation:
Permissible environment temperature
Permissible relative humidity
+5 ~ +40 °C
Below 31°C, max. humidity is 80%; at 40°C,
linearly decreases to 50%
Operating Parameters
Protection grade
II
Ingress protection
IP20
Electrical safety
Conforms to GB 4793.1-2007/ IEC 61010-
1:2001, including UL standard
Pollution index
2
Overvoltage category
II
Rated supply voltage
120V
Rated supply frequency
50Hz
Input power
6.5W
Light Sources
LED illumination:
Color temperature
Even illumination of field
Applicable objective
6000K –7000K
Diameter 4.5 mm
4X to 100X
Opto-mechanical parameters
Coaxial focus adjustment mechanism:
Coarse focus adjustment
Fine focus adjustment
0.02 mm/rotation

VWR International, LLC., Standard Biological Microscope
Stroke
0.02 mm/rotation
9mm
Nosepiece:
Manual quadruple nosepiece
Objective:
4X/10X/40X/100X
Eyepiece
Field number 18mm
Assembly diameter 23.2 mm
WF 10x/18
Mechanical tube length
160 mm
Mechanical stage: -
Dimension (L x W)
Stroke (L x W)
Position of vernier
Slide clip
1.25 condenser
120 x 120 mm
76 x 26 mm
On the right
On the left of movable clip
Used for 4X ~ 100X objectives
Movable stage: -
Dimension (L x W)
Max. moving range
Slide clip
0.65 condenser
110 x 110 mm
Ø 9mm
Linked slide clips
Used for 4X ~ 40X objectives
In the registered product standard, the electrical safety features of the biological microscope are:
overvoltage category II; pollution grade 2. The safety requirement test method and inspection rules for
this standard product are based on EN 61010-1:2001.