Star Lab RepeatOne User manual

Liquid HandLing TecHnoLogy
User Manual · Bedienungsanleitung · Mode d’emploi · Instruzioni d’impiego

www.starlabgroup.com
User Manual _______________________________ 3
Bedienungsanleitung _______________________ 15
Mode d’emploi ____________________________ 27
Instruzioni d’impiego_______________________ 39

English
RepeatOne · User Manual · 3
1. General ____________________________________ 4
1.1. Design Principle of RepeatOne® ____________________ 4
2. Volume Selection and Choice of TipOne® Repeat ___ 5
2.1. TipOne® Repeat Dispenser Tips _____________________ 6
2.2. 25 ml and 50 ml TipOne® Repeat Dispenser Tips ________ 6
3. Operation ___________________________________ 7
3.1. Inserting TipOne® Repeat Dispenser Tips ______________ 7
3.2. Filling TipOne® Repeat Dispenser Tips ________________ 7
3.3. Dispensing ___________________________________ 8
3.4. Removing TipOne® Repeat Dispenser Tips _____________ 9
4. Safety Precautions and Operational Limitations ___ 9
5. Troubleshooting ____________________________ 10
6. Maintenance, Service and Warranty ____________ 11
7. Ordering Information ________________________ 12
8. Technical Data ______________________________ 13
9. Quick Reference Guide _______________________ 14
User Manual

4· RepeatOne · User Manual
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1. General
RepeatOne® is a hand-held device for repetitive pipettings.
Up to 48 pipettings of the same sample can be made from
a single filling, resulting in a considerable reduction in time
and expense.
RepeatOne® allows the selection of five separate volumes
from each TipOne® Repeat Dispenser Tip. Eight different
TipOne® Repeat Dispenser Tip sizes provide the most
important volumes from 2 to 5000 µl.
Use only original STARLAB TipOne® Repeat Dispenser Tips
to guarantee the pipette’s performance.
1.1. Design Principle of RepeatOne® ( Fig.1)
Volume selection dial
To determine the pipetting volume, the dial setting
(1–5) is multiplied by the minimum pipetting volume
of the TipOne® Repeat Dispenser Tip.
Pipetting lever
The volume is dispensed by pressing the pipetting
lever down until it stops.
Filling lever
The TipOne® Repeat Dispenser Tip is filled by sliding
this lever upward.
Locking clamp
The locking clamp secures the TipOne® Repeat
Dispenser Tip firmly into RepeatOne®.
TipOne® Repeat Dispenser TipFig.1

English
RepeatOne · User Manual · 5
2. Volume Selection and Choice of TipOne® Repeat
For repetitive pipetting of a particular volume:
•Find the desired volume in the columns on the right.
•The required dial setting and maximum number of
pipetting steps for the volume are shown at the top
respectively buttom of the columns.
•Select the corresponding TipOne® Repeat Dispenser Tip
from the column on the left
*Adapter
necessary for
TipOne® Repeat
Dispenser Tip
25 ml and 50 ml.
Application Guide for TipOne® Repeat Dispenser Tips
TipOne® Repeat
Volume
Volume selection dial
12345
0.1 ml 2 μl 4 μl 6 μl 8 μl 10 μl
0.5 ml 10 μl 20 μl 30 μl 40 μl 50 μl
1.0 ml 20 μl 40 μl 60 μl 80 μl 100 μl
2.5 ml 50 μl 100 μl 150 μl 200 μl 250 μl
5.0 ml 100 μl 200 μl 300 μl 400 μl 500 μl
10 ml 200 μl 400 μl 600 μl 800 μl 1000 μl
25 ml* 500 μl 1.0 ml 1.5 ml 2.0 ml 2.5 ml
50 ml* 1.0 ml 2.0 ml 3.0 ml 4.0 ml 5.0 ml
48 23 15 11 8
Pipetting Steps

6· RepeatOne · User Manual
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2.1. TipOne® Repeat Dispenser Tips
The minimum pipetting volume and the maximum
filling capacity is stated on each TipOne® Repeat
Dispenser Tips (Fig. 2). The scale facilitates drawing off
partial quantities and helps you to estimate the amount
of liquid still available for operation.
The volume to be pipetted is obtained by multiplying
the set number located on the volume selection dial by
the minimum pipetting volume of the TipOne® Repeat
Dispenser Tips.
Eg. 1.0 ml TipOne® Repeat Dispenser Tips, Minimum volume:
1=20 µl at setting 4: 4 x 20 µl = pipetted volume: 80 µl
Better precision is achieved by using a TipOne® Repeat
Dispenser Tips of small capacity with a high dial setting.
2.2. 25 ml and 50 ml
TipOne® Repeat
Dispenser Tips (Fig.3)
Attach the adapter to the
25 ml or 50 ml TipOne®
Repeat Dispenser Tips by
turning it clockwise; re-
move by turning it counter
clockwise.
Number
of Pipetting
Steps
Position
of Volume
Selection Dial
48 1
23 2
15 3
11 4
8 5
Fig. 3
The correlation between
the position of the volume
selection dial and the
number of pipetting steps
is as shown in the table.
Fig. 2
2
1

English
RepeatOne · User Manual · 7
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1
2
Fig. 4
3. Operation
3.1. Inserting TipOne®
Repeat Dispenser Tips
•Slide the filling lever
down until it stops.
•Raise the locking clamp
upward.
•Insert the TipOne®
Repeat tip until it clicks
into position. Ensure
the TipOne® Repeat’s
plunger is fully inserted
into the barrel before
attaching it to the
RepeatOne® (Fig. 4). Fig. 5
•Ensure the filling lever is
completely down, and
then lower the locking
clamp to secure the
TipOne® Repeat tip in
place (Fig. 5).
3.2. Filling TipOne®
Repeat Dispenser Tips
Use the following steps
forall sizes of tips. 0.1 ml,
0.5 ml, 1.0 ml, and 2.5 ml
tips may also be used
with a standard 200 µl
pipette tip. Attaching a
tip facilitates filling from Fig. 6
narrow-necked vessels
or piercing of sealing foil
and also makes dispensing
of small volumes more
precise.
•Immerse the tip into the
liquid.
•Fill by slowly sliding the
filling lever upward
(Fig. 6).
•Discard the first
pipetting step.
RepeatOne® is now ready
for operation.

8· RepeatOne · User Manual
Additional Information
Sliding the filling lever too quickly can cause excessive
vacuum. This can cause tiny air bubbles to accumulate in
the liquid which may lead to dispensing inaccuracies.
Important: The first dispensing step must be discarded in
order to release any residual pressure from the dispensing
system after filling and to prepare the system for precise
dispensing.
Small bubbles in the TipOne® Repeat Dispenser Tip be-
neath the piston will not affect dispensing accuracy since
the residual stroke lock prevents the dispensing of any
remaining sample after the last dispense.
It is not necessary to completely fill the TipOne® Repeat
Dispenser Tip. Partially filling will not cause pipetting
inaccuracies.
3.3. Dispensing (Fig. 7)
•Check the volume
selection dial.
•Wipe the tip of the
TipOne® Repeat
Dispenser Tip with
lint-free tissue.
•Hold the dispensing tip
so it touches the inner
wall of the vessel.
•Dispense the liquid by
completely depressing
the dispensing lever.
Additional Information
Dispensing with a 5.0 ml or 10 ml TipOne® Repeat
Dispenser Tip is normally carried out without attaching
a pipette tip. Never attach a standard tip when working
with the 25 ml and 50 ml TipOne® Repeat tips.
A new TipOne® Repeat Dispenser Tip must be used each
time to avoid carry-over and contamination.
Fig. 7

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RepeatOne · User Manual · 9
3.4. Removing TipOne® Repeat Dispenser Tips (Fig. 8)
•Hold RepeatOne® over a beaker.
•Slowly depress the filling lever down to the stop.
This will completely empty the TipOne® Repeat
Dispenser Tip.
•Raise the locking clamp upward.
•Remove the TipOne® Repeat Dispenser Tip.
Note: When using the 25 ml or 50 ml TipOne® Repeat
Dispenser Tips, the reusable adapter is removed by turning
it counter clockwise.
Fig. 8
4. Safety Precautions and Operational Limitations
When removing TipOne® Repeat Dispenser Tips, please
note the following:
•Do not touch the tip end, especially when using
hazardous reagents!
•Empty the dispenser tips into a proper discard receptacle
to avoid splashing and aerosols.
•Treat any retained fluid as a bio-hazard. Do not allow the
fluid to come into contact with laboratory personnel.
•If dispensing a liquid with a surface tension significantly
different from water, inaccuracies or leaking can occur.
•The RepeatOne® with an attached, partially filled
TipOne® Repeat Dispenser Tip should be stored in a
stand to prevent air entering the dispenser tip.

10 · RepeatOne · User Manual
5. Troubleshooting
Error Cause Solution
TipOne® Repeat Dispenser Tip cannot be inserted. Filling lever not fully in the down position;
therefore, locking clamp cannot be completely raised. Depress the filling lever downward and raise the locking clamp as far as it will go.
The dispenser tip piston is not completely inserted in the barrel. Slide the piston into the proper position.
Unable to fully lift the filling lever. Locking clamp incompletely depressed. Completely depress the locking clamp.
Excessive air bubbles when filling the TipOne® Repeat Dispenser Tip. Filling lever lifted slightly before immersing into the liquid. Draw up some solution and then discard it (using filling lever).
Then refill the TipOne® Repeat Dispenser Tip.
Leaking of TipOne® Repeat Dispenser Tip. Replace the dispenser tip with a new one.
Inconsistent pipetting. Volume selection dial has not clicked into position. Click volume selection dial into position.
Dripping TipOne® Repeat Dispenser Tip. Leaking TipOne® Repeat Dispenser Tip. Replace with a new dispenser tip.
Attached TipOne® Repeat Dispenser Tip is loose. Use more force to attach the TipOne® Repeat Dispenser Tip.
RepeatOne® jams or does not function. Return the RepeatOne® for repair or replacement.

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RepeatOne · User Manual · 11
6. Maintenance, Service and Warranty
Maintenance
External surfaces of RepeatOne® can be cleaned with
warm water or isopropyl alcohol.
Apart from the need to clean externally, the RepeatOne®
is completely maintenance free.
Important: under no circumstances should the
RepeatOne® be dismantled!
RepeatOne® and TipOne® Repeat Dispenser Tips are not
autoclavable!
Service
If a problem cannot be solved with the aid of the Trou-
bleshooting Guide, return RepeatOne® to your regional
STARLAB office or distributor for repair or replacement.
Important: Please decontaminate the dispenser before
return. Proper decontamination has to be confirmed in a
separate “declaration of decontamination”.
Please contact STARLAB for more detailed information.
Warranty
Please check that the unit has been received complete and
undamaged. Save all packaging and documents until you
have thoroughly inspected your shipment; if you find that
your order is incorrect or damaged please call STARLAB
for instructions for its return.
This warranty is valid for 24 months, but only if the prod-
uct has been used and cared for according to this User
Manual. No liability is accepted for loss or damage arising
from incorrect use.
The liability is limited to the repair or replacement of the
unit, or a refund of the purchase price, at STARLAB’s op-
tion. STARLAB is not liable for any consequential damages.
We refer to our general terms of sale. STARLAB reserves
the right to alter the specifications without prior notice.

12 · RepeatOne · User Manual
Important Note:
Please only use the accessories recommended by STARLAB. Using
non-recommended disposables can reduce the precision and accu-
racy, and life, of RepeatOne®. We do not honour any warranty or
accept any responsibility for damage resulting from such action.
RepeatOne® Dispenser and Adapter
Cat-No. Description Pack Size
S4026-1500 RepeatOne® Dispenser 1
S4756-2500 25 ml Adapter 1
S4756-5000 50 ml Adapter 1
S4765-2500 25 ml Adapter (sterile) 1
S4765-5000 50 ml Adapter (sterile) 1
TipOne® Repeat Dispenser Tips
Cat-No. Description Pack Size
S4751-0010 0.1 ml TipOne® Repeat Dispenser Tips 100
S4751-0050 0.5 ml TipOne® Repeat Dispenser Tips 100
S4751-0100 1.0 ml TipOne® Repeat Dispenser Tips 100
S4751-0250 2.5 ml TipOne® Repeat Dispenser Tips 100
S4751-0500 5.0 ml TipOne® Repeat Dispenser Tips 100
S4751-1000 10 ml TipOne® Repeat Dispenser Tips 100
S4751-2500 25 ml TipOne® Repeat Dispenser Tips 100
S4751-5000 50 ml TipOne® Repeat Dispenser Tips 100
TipOne® Repeat Dispenser Tips (sterile)
Cat-No. Description Pack Size
S4761-0010 0.1 ml TipOne® Repeat Dispenser Tips (sterile) 100
S4761-0050 0.5 ml TipOne® Repeat Dispenser Tips (sterile) 100
S4761-0100 1.0 ml TipOne® Repeat Dispenser Tips (sterile) 100
S4761-0250 2.5 ml TipOne® Repeat Dispenser Tips (sterile) 100
S4761-0500 5.0 ml TipOne® Repeat Dispenser Tips (sterile) 100
S4761-1000 10 ml TipOne® Repeat Dispenser Tips (sterile) 100
S4761-2500 25 ml TipOne® Repeat Dispenser Tips (sterile) 100
S4761-5000 50 ml TipOne® Repeat Dispenser Tips (sterile) 100
7. Ordering Information

English
RepeatOne · User Manual · 13
8. Technical Data
Test conditions in accordance with EN ISO 8655
Liquid: Water bi-distilled, degassed
Test Temperature: 20–25°C, ±0.5 % constant
Operating Temperature: +4 °C to +40 °C
Measurement: Performed with original TipOne®
Repeat Dispenser Tips in accor-
dance with EN ISO 8655
Technical specifications are subject to change without
notice!
TipOne® Repeat
Dispenser Tip Size
Dispensing Volume Systematic Error (Inaccuracy) Random Error (Imprecision; CV)
Minimum Maximum Minimum Maximum Minimum Maximum
0.1 ml 2 μl 10 μl ± 1.6 % ± 1.6 % ≤5.0 % ≤2.5 %
0.5 ml 10 μl 50 μl ± 1.0 % ± 1.0 % ≤1.5 % ≤0.8 %
1.0 ml 20 μl 100 μl ± 0.9 % ± 0.9 % ≤0.9 % ≤0.6 %
2.5 ml 50 μl 250 μl ± 0.8 % ± 0.8 % ≤0.8 % ≤0.5 %
5.0 ml 100 μl 500 μl ± 0.6 % ± 0.6 % ≤0.6 % ≤0.3 %
10 ml 200 μl 1000 μl ± 0.5 % ± 0.5 % ≤0.6 % ≤0.3 %
25 ml 500 μl 2500 μl ± 0.4 % ± 0.4 % ≤0.6 % ≤0.25 %
50 ml 1000 μl 5000 μl ± 0.3 % ± 0.3 % ≤0.5 % ≤0.25 %

14 · RepeatOne · User Manual
www.starlabgroup.com
9. Quick Reference Guide
•Look for desired volume
in the volume selection
chart (See page 5).
•Select appropriate
TipOne® Repeat tip by
number of dispensing
steps.
•Set volume selection
dial to corresponding
position.
•Slide filling lever down.
•Raise locking clamp
upward.
•Insert TipOne® Repeat
tip into position.
•Lower locking clamp
and press closed.
•Immerse the tip of t
he TipOne® Repeat tip
into liquid.
•Slide filling lever
upward.
•Discard first dispensing
step.
•Dispense.
•Empty TipOne® Repeat
tip completely.
•Raise locking clamp
upward.
•Remove TipOne® Repeat
tip.
Important: The Quick Reference Guide should only be
used after the operator is thoroughly acquainted with the
detailed instructions, otherwise errors may occur.

RepeatOne · Bedienungsanleitung · 15
Deutsch
1. Einführung _________________________________ 16
1.1. Funktionsprinzip ______________________________ 16
2. Volumenwahl und Auswahl von TipOne® Repeat __ 17
2.1. TipOne® Repeat Dispenserspitzen __________________ 18
2.2. TipOne® Repeat 25 ml und 50 ml Dispenserspitzen _____ 18
3. Bedienung _________________________________ 19
3.1. Einsetzen von TipOne® Repeat Dispenserspitzen _______ 19
3.2. Füllen von TipOne® Repeat Dispenserspitzen __________ 19
3.3. Dosieren ____________________________________ 20
3.4. Entnahme von TipOne® Repeat Dispenserspitzen _______ 21
4. Sicherheitshinweise und Anwendungsgrenzen ____ 21
5. Fehlerbehandlung ___________________________ 22
6. Wartung, Service und Garantie ________________ 23
7. Bestellinformationen ________________________ 24
8. Technische Daten ___________________________ 25
9. Kurzanleitung ______________________________ 26
Bedienungsanleitung

16 · RepeatOne · Bedienungsanleitung
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Abb.1
1. Einführung
RepeatOne® ist ein Handdispenser für Seriendosierungen.
Eine größere Reagenzmenge kann in einem Arbeitsgang
aufgenommen und schrittweise in schneller Folge wieder
abgegeben werden. Daraus ergibt sich eine beträchtliche
Reduzierung des Arbeitsaufwandes.
Mit 5 Hubeinteilungen und 8 verschiedenen TipOne®
Repeat Dispenserspitzen Größen werden mit RepeatOne®
die wichtigsten Volumina im Bereich von 2 – 5000 µl
dosiert.
Die Richtigkeit der Dosierung garantieren wir bei Verwen-
dung von Original TipOne® Repeat Dispenserspitzen.
1.1. Funktionsprinzip (Abb.1)
Drehknopf zur Volumenwahl
Durch Multiplikation der Zahlen 1 bis 5 mit dem kleins-
ten Dosierschritt einer TipOne® Repeat Dispenserspitze
ergibt sich das Dosiervolumen.
Dosierhebel
Durch Druck bis zum Anschlag wird das Dosiervolumen
abgegeben.
Füllhebel
Durch Hochschieben wird die TipOne® Repeat
Dispenserspitze gefüllt.
Arretierhebel
Dient zum festen Fixieren der TipOne® Repeat
Dispenserspitzen.
TipOne® Repeat Dispenserspitze

RepeatOne · Bedienungsanleitung · 17
Deutsch
2. Volumenwahl und Auswahl von TipOne® Repeat
Um ein bestimmtes Volumen zu dosieren, gehen Sie bitte
folgendermaßen vor:
•Suchen Sie das gewünschte Dosiervolumen in den
Spalten rechts (weiß unterlegt).
•Die erforderliche Drehknopfposition und die maximale
Anzahl der Abgabeschritte für das jeweilige Volumen
finden Sie am oberen bzw. unteren Rand der Spalte
(hellblau unterlegt).
•Am linken Tabellenrand finden Sie die entsprechende
TipOne® Repeat Dispenserspitzen Größe (hellgelb
unterlegt). *TipOne® Repeat
Dispenserspitzen
25 ml/50 ml
nur mit Adapter
verwendbar.
Dosiervolumen TipOne® Repeat Dispenserspitzen
TipOne® Repeat
Volumen
Drehknopfposition
12345
0,1 ml 2 μl 4 μl 6 μl 8 μl 10 μl
0,5 ml 10 μl 20 μl 30 μl 40 μl 50 μl
1,0 ml 20 μl 40 μl 60 μl 80 μl 100 μl
2,5 ml 50 μl 100 μl 150 μl 200 μl 250 μl
5,0 ml 100 μl 200 μl 300 μl 400 μl 500 μl
10 ml 200 μl 400 μl 600 μl 800 μl 1000 μl
25 ml* 500 μl 1,0 ml 1,5 ml 2,0 ml 2,5 ml
50 ml* 1,0 ml 2,0 ml 3,0 ml 4,0 ml 5,0 ml
48 23 15 11 8
Abgabeschritte

18 · RepeatOne · Bedienungsanleitung
2
1
Anzahl
der Abgabe-
schritte
Drehknopf-
position
48 1
23 2
15 3
11 4
8 5
Abb. 3Abb. 2
2
1
2.1. TipOne® Repeat Dispenserspitzen
Auf TipOne® Repeat (Abb. 2) ist der kleinste Dosierschritt
und das max. Füllvolumen angegeben. Die Skala
erleichtert das Aufsaugen von Teilmengen und ermöglicht
während des Arbeitens das Abschätzen der noch vorhan-
denen Flüssigkeitsmenge.
Durch Multiplikation der eingestellten Drehknopfposition
mit dem kleinsten Dosierschritt des gewählten TipOne®
Repeat Dispenserspitzen erhält man das Dosiervolumen,
z.B. TipOne® Repeat 1,0 ml, 1= 20 µl, Einstellung 4 =
4 x 20 µl = 80 µl Dosiervolumen pro Abgabeschritt.
Dabei ist zu bedenken, daß die Richtigkeit der Dosierung
besser wird, wenn das Volumen durch eine kleinere
TipOne® Repeat Dispenserspitze bei hoher Drehknopf-
position dosiert wird.
2.2. TipOne® Repeat
25 ml und 50 ml Dis-
penserspitzen (Abb.3)
Der Adapter wird mit
Hilfe eines Bajonett-Ver-
schlusses auf dem ent-
sprechenden 25 ml oder
50 ml Tip befestigt und
nach Gebrauch wieder
abgeschraubt.
Die Korrelation zwischen
der Anzahl der Abgabe-
schritte und der Drehknopf-
position ist in der Tabelle
ersichtlich.

RepeatOne · Bedienungsanleitung · 19
Deutsch
1
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1
2
Abb. 4
3. Bedienung
3.1. Einsetzen von
TipOne® Repeat
Dispenserspitzen
•Füllhebel bis zum
Anschlag nach unten
schieben.
•Arretierhebel
hochklappen.
•TipOne® Repeat
einrasten (Abb. 4).
•Füllhebel nochmals leicht
nach unten schieben und
Arretierhebel herunter-
drücken (Abb.5). Abb. 5
3.2. Füllen von TipOne® Repeat Dispenserspitzen
Führen Sie die folgenden Schritte für alle Spitzengrößen
aus. 0,1 ml, 0,5 ml, 1,0 ml und 2,5 ml Spitzen können
auch mit einer Standard 200 µl Pipettenspitze verwendet
werden. Das Aufsetzen einer Spitze erleichtert das Be-
füllen aus enghalsigen Gefäßen oder das Durchstechen
von Verschließfolien und macht das Dispensieren kleiner
Volumina präziser.
•Spitze in die Flüssigkeit eintauchen.
•Füllen durch langsames Hochschieben des Füllhebels
(Abb. 6).
•Ersten Dosierschritt abgeben und verwerfen.
Jetzt ist RepeatOne® arbeitsbereit.
Abb. 6

20 · RepeatOne · Bedienungsanleitung
Zusatzinformationen
Das Hochschieben des Füllhebels sollte nicht schnell
geschehen, um eine Anreicherung von Luftblasen in der
Flüssigkeit infolge zu starken Unterdruckes zu verhindern.
Luftblasen können zu Dosierungenauigkeiten führen.
Wichtig: Der erste Dosierschritt muss verworfen werden,
um das Dosiersystem nach dem Füllen zu entspannen und
zur exakten Dosierung vorzubereiten.
Eine im oberen Zylinderraum sitzende Luftblase stört nicht.
Sie hat keinen Einfluss auf die genaue Dosierung, da der
letzte Abgabeschritt durch die Resthubsperre nicht mehr
dosiert wird.
TipOne® Repeat muss nicht grundsätzlich vollständig
gefüllt werden. Die Dosiergenauigkeit wird dadurch nicht
beeinflusst.
3.3. Dosieren (Abb.7)
•Volumeneinstellung
überprüfen.
•Spitze des TipOne®
Repeat mit faserfreiem
Zellstoff abwischen.
•Spitze an die
Gefäßwand anlegen.
•Reagenz dosieren durch
Herunterdrücken des
Dosierhebels bis zum
Anschlag
Zusatzinformationen
Die Dosierung mit TipOne® Repeat 5,0 ml und 10 ml
erfolgt normalerweise ohne Spitze. TipOne® Repeat 25 ml
und 50 ml müssen ohne Spitze dosiert werden.
Beim Wechsel des Reagenzes ist TipOne® Repeat auszu-
tauschen.
Abb. 7