Sutter Instrument MP-285 Assembly instructions

One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: .sutter.com
Fax: [phone] Email: [e-mail]
MP-285
Micromanipulator System
ROE
Basic Operations
Rev. 3.08 ( 20140809)

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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Copyright © 2014 Sutter Instrument Company. All Rights Reserved.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
iii
DISCLAI ER
The P-285-system consists of one controller ( P-285), a user control device (ROE or
Joystick), and one electromechanical micromanipulator device ( P-285/ ). The purpose of
the system is for the manipulation at the micro level of micropipettes and probes used in
conjunction with a microscope. No other use is recommended.
This instrument is designed for use in a laboratory environment. It is not intended, nor
should it be used in human experimentation or applied to humans in any way. This is not a
medical device.
Do not open or attempt to repair the instrument. High voltages are present and inadvertent
movement of the micromanipulator electromechanical could cause injury.
Do not allow unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAFETY WARNINGS AND PRECAUTIONS
Electrical
Operate the P-285 using 110-- 240 V AC., 50-60 Hz line voltage. This instrument is
designed for use in a laboratory environment that has low electrical noise and mechanical
vibration. Surge suppression is recommended at all times.
 Fuse Replacement: Replace only with the same type and rating:
ains fuse type and rating.
Fuse
(Type: Time Delay, 5mm x 20mm, glass tube)
ains Voltage
Setting
Rating anufacturer Examples
“110”
(100 – 120
VAC)
2A, 250V
(Time Delay)
Bussmann: G C-2A, G C-2-R (RoHS), GDC-2A, or
S506-2A (RoHS)
Littelfuse: 239 002 or 239.002.P (RoHS)
“220”
(200 – 240
VAC)
T1.0A, 250V Bussmann: GDC-1A or S506-1A (RoHS)
Littelfuse: 218 001 or 218 001.P (RoHS)
A spare fuse is located in the power input module. Please refer to the fuse-replacement
appendix for more details on fuse ratings and for instructions on how to change the fuse.
Avoiding Electrical Shock and Fire-related Injury

Always use the grounded power supply cord set provided to connect the system to a
grounded outlet (3-prong). This is required to protect you from injury in the event that
an electrical hazard occurs.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
iv

Do not disassemble the system. Refer servicing to qualified personnel.

To prevent fire or shock hazard do not expose the unit to rain or moisture.
Electromagnetic Interference
To comply with FDA and CE electromagnetic immunity and interference standards; and to
reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided with the unit for the interconnection
of the ROE, P-285/ electromechanical device, and host computer via serial RS-232
interface, (see the Technical Specifications appendix for more details).
Operational
Failure to comply with any of the following precautions may damage this device.

This instrument is designed for operation in a laboratory environment (Pollution Degree
I) that is free from mechanical vibrations, electrical noise and transients.

This unit is not designed for operation at altitudes above 2000 meters nor was it tested
for safety above 2000 meters.

DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE
CONTROLLER AND THE ECHANICAL UNITS WHILE POWER IS ON.
Please allow at least 20 seconds after turning the unit off before disconnecting the
mechanical units. Failure to do this may result in damage to the electronics.

Operate this instrument only according to the instructions included in this manual.

Do not operate if there is any obvious damage to any part of the instrument.

Operate only in a location where there is a free flow of fresh air on all sides.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.

Do not operate this instrument near flammable materials. The use of any hazardous
materials with this instrument is not recommended and if undertaken is done so at the
users’ own risk.

Do not attempt to operate the instrument with the manipulator shipping screws in
place - severe motor damage may result.

Do not operate if there is any obvious damage to any part of the instrument. Do not
attempt to operate the instrument with the manipulator shipping screws in place - severe
motor damage may result. When transporting the mechanical manipulator, be sure to
install the shipping screws supplied in their correct locations. Failure to do this may
result in damage to the motors.

Never touch any part of the micromanipulator electromechanical device while it is in
operation and moving. Doing so can result in physical injury (e.g., fingers can be caught
and pinched between the moving parts of the micromanipulator).

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
v

As with all microinjection devices, sharp micropipettes can fly out of their holder
unexpectedly. Always take precautions to prevent this from happening. Never loosen the
micropipette holder chuck when the tubing is pressurized, and never point micropipette
holders at yourself or others. Always wear safety glasses when using sharp glass
micropipettes with pressure microinjectors.

Do not handle the manipulator mechanical while the power is on, and take care to
ensure no cables pass close to the mechanical manipulator.
Other

Use this instrument only for microinjection purposes in conjunction with the procedures
and guidelines in this manual.

Retain the original packaging for future transport of the instrument.

Some applications, such as piezo-impact microinjection call for the use of mercury in the
micropipette tip. The use of any hazardous materials with any Sutter Instrument’s
instrument is not recommended and if undertaken is done so at the users’ own risk.

When transporting the mechanical manipulator, be sure to install the shipping screws
supplied in their correct locations. Failure to do this may result in damage to the motors.

This instrument contains no user-serviceable components — do not open the instrument
casing. This instrument should be serviced and repaired only by Sutter Instrument or an
authorized Sutter Instrument servicing agent.

Sutter Instrument reserves the right to change specifications without prior notice.

This device is intended only for research purposes.
Handling icropipettes
Failure to comply with any of the following precautions may result in injury to the users
of this device as well as those working in the general area near the device.

The micropipettes used with this instrument are very sharp and relatively fragile.
Contact with the pulled micropipette tips, therefore, should be avoided to prevent
accidentally impaling yourself.

Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps”
container.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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TABLE OF CONTENTS
DISCLAI ER ......................................................................................................................................iii
SAFETY WARNINGS AND PRECAUTIONS...................................................................................iii
Electrical .................................................................................................................................................iii
Avoiding Electrical Shock and Fire-related Injury.............................................................................iii
Electromagnetic Interference...............................................................................................................iv
Operational .............................................................................................................................................iv
Other.........................................................................................................................................................v
Handling icropipettes..........................................................................................................................v
1. GENERAL INFOR ATION ...........................................................................................................1
1.1 Structure of the P-285 Documentation Package ...............................................................................1
1.2 Technical Support.............................................................................................................................1
2. SETUP...............................................................................................................................................3
2.1 Unpacking..........................................................................................................................................3
2.2 Initial Setup and Gross ovement.................................................................................................4
2.3 Installation of the P-285 icromanipulator on your icroscope............................................6
2.3.1 General Considerations .............................................................................................................6
2.3.2 Specifics.......................................................................................................................................6
2.4 ounting Headstages.......................................................................................................................7
2.5 Adjusting the Rotating Dovetail Base.............................................................................................8
2.6 inimizing Electrical Noise ............................................................................................................9
3. ICROSCOPIC ANIPULATION ..............................................................................................11
3.1 Some Basic Information.................................................................................................................11
3.2 SETUP Selection ............................................................................................................................11
3.3 Changing inimal Resolution.......................................................................................................12
4. PULSE OVE ENT.....................................................................................................................15
5. HO E FUNCTION........................................................................................................................17
5.1 How to Define a New Home ..........................................................................................................17
5.2 oving to the New Home:.............................................................................................................17
5.3 Practical Use of the Home Function.............................................................................................17
6. GENERALIZED ROBOTIC OVE ENTS.................................................................................19
6.1 Setting Absolute Zero.....................................................................................................................19
6.2 Redefining Home ............................................................................................................................19
6.3 Clearing Program Location #1 for Recording a New Robotic Series .......................................19
6.4 Teaching the P-285 Controller a New Robotic Series.............................................................20
6.5 Executing the Newly Entered Program!......................................................................................21
6.6 Loading a Saved Program..............................................................................................................22
7. DIAGONAL OR 4TH AXIS OVE ENTS .................................................................................23
8. ROE RESPONSE CHARACTERISTICS ......................................................................................25
8.1 Controlling ROE Knob to anipulator Stepper otor Axis Assignment................................25
8.2 Controlling ovement Amount per ROE Turn..........................................................................25

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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APPENDIX A. LI ITED WARRANTY............................................................................................27
INDEX.................................................................................................................................................29
TABLE OF FIGURES
Figure 2-1. P-285 Controller (front panel). ..........................................................................................3
Figure 2-2. anipulator. Figure 2-3. Rotary Optical Encoder (ROE)...............................................3
Figure 2-4. P-285 controller rear connections. .................................................................................... 4
Figure 2-5. anipulator base dimensions................................................................................................6
Figure 2-6. Headstage mounting plate..................................................................................................... 7
Figure 2-7. ounting the headstage on the manipulator's Z-axis.........................................................8
Figure 2-8. Adjusting the headstage angle............................................................................................... 8
TABLE OF TABLES
Table 3-1. icrosteps per ROE turn settings........................................................................................12
Table 3-2. icrons per ROE turn settings.............................................................................................13
Table 4-1. icrosteps and microns per Pulse settings. ........................................................................16

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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1. GENERAL INFOR ATION
1.1 Structure of the P-285 Documentation Package
The P-285 is a highly versatile stepper motor driven micromanipulator. Because of this
versatility, the documentation necessary to cover various functions has rapidly grown to a
size best described as unwieldy and thus has generally not been useful to most operators.
In order to produce a more useful set of instructions, we have split the manual into two
parts. The first part, “Basic Operations”, has been kept short and covers major functions
with the aim of quickly guiding the first time user through the setup and use of the P-285
for micromanipulation with emphasis on using the ROE (Rotary Optical Encoder) for user
control. These steps are, in many ways, identical to the steps performed any time one sets up
an P-285 for use at a microscope. any users will find that no other manual is necessary.
The second part, “Reference anual”, provides in-depth coverage of the P-285. It
documents the precise keystrokes (menu navigation) necessary to access each P-285
function. The Reference anual also describes, in detail, how to alter P-285 performance
via add-on accessories, perform microcontroller robotic programming, and how to use the
serial computer interface.
1.2 Technical Support
Unlimited technical support is provided by Sutter Instrument Company at no charge to our
customers. Our technical support staff is available between the hours of 8:00 A and 5:00
P (Pacific Time) at [phone]. You may also E-mail your queries to [e-mail].

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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2. SETUP
2.1 Unpacking
Carefully remove all components from the shipping container. One of each of the following
should be included:
 P-285 controller
 P-285 micromanipulator and D25 cable for connection to controller
 Rotary Optical Encoder (ROE) and D15 cable for connection to controller
 Controller power cord
 Serial port cable (DB9 connectors)
 etal dovetail headstage mounting plate with plastic isolation plate
 etal dovetail rod clamp
 2.5mm hex wrench for removing the shipping screws
 7/64 hex wrench for adjusting the rotating dovetail base
 P-285 documentation
POWER
SUTTER INSTRUMENT CO.
MADE IN U.S.A.
MP-285
RESET
Figure 2-1. P-285 Controller (front panel).
0
4
1
3
3
1
4
1
3
3
1
0
4
1
3
3
1
4
1
3
3
1
HOM
NOR
COAR
CON
DIA
FIN
PULS
Figure 2-2. anipulator. Figure 2-3. Rotary Optical Encoder (ROE).

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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You may have also received one or more accessories for mounting your P-285 and/or
modifying the headstage mount to the manipulator (i.e., rotating base, microscope stage
mount, gantry stand, dovetail extension). Setup of these accessories is covered either in
documentation accompanying the accessory or in the P-285 Reference anual .
Remove all six shipping screws from the P-285. Save the screws, warning tags, and hex
wrench in the event you need to transport your manipulator in the future. Once these screws
have been removed, handle the micromanipulator with care. The drive cables can be
damaged
2.2 Initial Setup and Gross ovement
Initially, you may want to simply connect your manipulator, controller, and ROE together and
try some gross movements in order to get a feel for the controls and how to make simple
movements. It is perfectly acceptable to set the manipulator in the middle of a bench top,
make all electrical connections and then observe the unit’s movement by eye. Even if you
wish to directly install your manipulator in your rig, it is useful to follow the initial setup
procedure in order to learn how to move the unit and allow easy access to the mounting
screws if subjected to unnecessary strain.
1. Connect the power cord to the P-285 controller. ake certain that the voltage selector
next to the power cord socket on the back of the P-285 controller has been set to the
appropriate voltage (110 or 220).
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
110
220
SERIAL PORT
CABLE (9 pin)
INPUT DEVICE
CABLE (15 pin)
LINE POWER VOLTAGE
SELECTOR
FUSE
FUSE
L
ABEL
MANIPULATOR
CABLE (25 pin)
SUTTER
INSTRUMENT
51 Digita Drive
NOVATO, CA 94949 USA
(415) 883
-
0128
DISCONNECT POWER
BEFORE OPENING
UNIT!
MODEL
Hz
FUSE
VOLTS
HIGH VOLTAGE
DANGER
SERIAL PORT
MANIPULATOR
COMMAND INP
UT
Figure 2-4. P-285 controller rear connections.
2. With the power OFF (front panel switch in the down position), use the D15 cable and
connect the ROE to the connection labeled “CO AND INPUT” on the back of the P-
285 controller.
CAUTION: Never connect or disconnect the ROE while the power is on!
3. With the power OFF, use the D25 cable and connect the P-285 micromanipulator to the
DB 25 connector labeled “ ANIPULATOR” on the back of the controller.
CAUTION: Never connect or disconnect the micromanipulator while the power is on!

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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CAUTION: Important! Check to make sure that you have removed all six shipping screws.
The unit can be severely damaged if you try to move one of the axes with the shipping screws
in place.
To observe movement of the manipulator do the following:
1. Turn the power ON (front panel switch up). Observe the startup screen:
2. Press the < OVE> key on the P-285 controller keypad. Observe the movement screen:
Assuming this was the initial power on from the unit after unpacking, you should have
coordinates similar to those above (-10,000µm to -12,000µm). These coordinates correspond
to the locations of the shipping screws.
Turn a knob on the ROE. You should see one of the axes on the manipulator move and the
coordinates on the screen of the controller change. The coordinates indicate how far you have
moved in micrometers along each of the three axes.
Before the unit was shipped from Sutter, its absolute origin (location 0,0,0) was set at the
center of travel of each axis. This same location was also defined as “Home”. This means that
you can make the manipulator move to the center of travel by a simple move to “Home”, as
follows:
1. ake sure you are still in the movement screen (coordinates displayed).
2. Press the button on the ROE marked <HO E>.
Observe the “rapid” movement of the manipulator to the center of travel of each axis. The
controller display will read, “Going home” during the move, and will display coordinates
close to 0,0,0 after the move is complete.
The P-285 controller front panel is configured with three colored LEDs. When lit, these
LEDs indicate that a signal is being received from the input device. Each LED (yellow, red,
and green) indicates input originating from one of the three knobs on the ROE (top, left, and
right, respectively).
S

1 * * * M P - 2 8 5 C o n t r o l l e r * * * P

1
P r e s s M O V E , P R G M o r s e l e c t m a g . w i t *
- 1 0 7 8 9 . 2 0 u m X - 1 0 7 7 8 . 2 4 u m Y
- 1 0 7 5 0 . 4 0 u m Z

0.20 um Res.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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2.3 Installation of the P-285 icromanipulator on your icroscope
2.3.1 General Considerations
In most applications, it is best to try to mount the P-285 in a location that allows for the
widest range of movement. Usually, this means mounting so that the device is in the center
of travel when it is in its normal operating position. To facilitate this procedure, move the
manipulator to its center of travel before determining where it should be mounted.
The base of the P 285 manipulator is also the mounting surface for the bearing ways of the
X-axis. When the base plate is pressed firmly against most mounting surfaces, torsional
stress can be transmitted to the base and thus misaligns the bearing ways. A supply of four
precision-thickness washers is included with your P-285 manipulator. To limit
misalignment, you should use one on each of the four mounting screws between the P-285
manipulator base and your mounting surface.
When mounting the P-285 there should be no physical impediments to the full excursion of
the three axes. Pay particular attention to the screws or clamps used to secure the base of the
micromanipulator to make certain that the X-axis slide can move to the end of its travel
without obstruction.
Additional mounting considerations, as well as instructions pertaining to specific mounting
accessories can be found in the Reference anual.
2.3.2 Specifics
The base of the P-285 manipulator has four mounting holes on 2.5” centers. A template,
drawn to scale and based on the footprint shown below, can be used to drill holes in a suitable
mounting surface.
3.8125”
2.75”
2.75”
2.5”
2.5”
2.25”
Figure 2-5. anipulator base dimensions.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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This four-hole pattern is also compatible with the P-285 rotary base, part #285RBI and the
mounting adapter plate, # 285210 (see the Reference anual for further instructions).
While it is possible to insert and tighten all four mounting screws with the P-285 in one
position, you may find it convenient to connect and power up the manipulator and position
the X-axis for the best access to the screw holes.
2.4 ounting Headstages
The most stable way to mount a headstage is to fasten it directly to the Z-axis slide. The
mounting hardware included with the P-285 is designed to eliminate the need for custom
machining of headstage and pipette holders. For example, Axon Instruments’ new 203B
headstage mounts directly to the rotating dovetail on the P-285 Z-axis. Other headstages
require use of the dovetail mounting plate:
dovetai mounting p ate:
2 midd e (D) ho es
EPC: 1
-
1/16" x 2
-
7/32"
(2 ho es, A & B)
CV-4: 1-1/8" x 1-15/16"
(4 ho es, B & C)
A
B
C
C
C
D
D
Figure 2-6. Headstage mounting plate.
The plastic portion of the plate provides electrical isolation while allowing for a direct mount
of Axon Instruments’ CV-4 headstage or Heka Electronics’ EPC-7, 8, or 9 headstages. The
user can drill additional holes to allow the plate-mount installation of other headstages.
Headstage mounting instructions can be found in the Reference anual.
Whether using the Axon 203B Headstage or another headstage mounted on the dovetail
mounting plate, the respective male dovetail is inserted into the female dovetail on the P-
285 Z-axis. Slide the dovetail mounting plate/headstage assembly into the rotating-dovetail
base and secure it by tightening the dovetail clamp set screw with a Phillips-head
screwdriver.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
8
Rotating dovetai
Headstag
e
Dovetai mounting
p ate
Dovetai c amp set
screw
P astic iso ation
p ate
Figure 2-7. ounting the headstage on the manipulator's Z-axis.
2.5 Adjusting the Rotating Dovetail Base
The rotating dovetail base allows the pipette holder or headstage to be rotated and firmly
fixed at any angle desired by the experimenter as required for access to a particular
microscope.
0
15
15
0
45
30
30
15
45
15
30
30
15
45
15
30
30
15
45
15
30
30
Headstage
Loosen then
retighten
Rotate
Figure 2-8. Adjusting the headstage angle.
To adjust the rotary dovetail to your desired angle:
1. Loosen the brake set screw to rotate the headstage or pipette holder. Failure to loosen the
screw may lead to rapid deterioration of the brake and rotating disk thereby reducing the
ability to firmly secure the rotating dovetail.
2. Once you have determined the proper angle, firmly tighten the brake set screw to assure
stable positioning of the rotating dovetail.
It is not recommended that you use this rotational joint as a means of pipette replacement.
Repeated repositioning of the rotary disk and brake will lead to rapid deterioration of the
joint which may then not tighten properly to allow stable positioning of a pipette.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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2.6 inimizing Electrical Noise
We are aware of two potential noise sources for users coupling their P-285 with high-gain,
high input impedance, electrophysiological recording amplifiers. The first is associated with
the manipulator and its connecting cable from the controller. Under certain circumstances
these may act as an antenna concentrating electric field noise that originates from nearby
electrical equipment (computer monitors and fluorescent lights are the most notorious
offenders) and bringing it into close proximity with recording apparatus. Grounding the
manipulator will largely eliminate this noise source. Ground tabs are located on each motor
housing for this purpose. It should be noted that the manipulator produces negligible
electrical noise when it is not moving because it is powered by a linear power supply with no
AC current present.
The second potential source of noise is associated with the magnetic field radiated from the
power supply transformer in the controller. Certain electrophysiological amplifiers are more
susceptible to generating noise when exposed to this magnetic field. This noise can be
minimized or eliminated by allowing a maximal distance between your P-285 controller and
your amplifier. It may be possible to decrease this noise source further by placing a soft steel
plate or a piece of high permeability metal between the controller and the amplifier.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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3. ICROSCOPIC ANIPULATION
3.1 Some Basic Information
Assuming you have already tried gross movement of the P-285 as outlined above (Initial
Setup and Gross ovement) then you have an understanding of most of the features
necessary for microscopic manipulation using your P-285. The controller makes two
changes to implement fine movement:
 The number of microsteps commanded per revolution of the ROE is reduced.
 The size of the microsteps produced by the stepping motor (minimal resolution) is
reduced from 0.2mm to 0.04mm.
These two changes give you a wide range of control over the amount of movement
commanded by a single turn of an ROE knob. In the gross movement mode, you are
commanding slightly over 700mm/turn. In the precise movement mode, you can command as
few as 20mm/turn.
As with the other portions of this User’s anual, it is hoped that enough information has
been provided to get the average user “micromanipulating” with a minimum of time spent
covering unnecessary detail. If you find yourself needing additional detail on any of the items
covered, please consult the appropriate topic in the Reference anual.
3.2 SETUP Selection
In order for the P-285 to make precise movements and have a greater amount of control
over the movement, you instruct the controller to produce fewer microsteps (the minimal
increment of manipulator movement) per rotation of the ROE knobs. Using simple
commands, you choose one of four different “Setups” that vary the number of microsteps per
turn of the ROE knob over a range of 512 to 5120 per turn.
The following steps outline how to use your P-285 to manipulate a pipette into position in
the field of view of your microscope. In doing so, you will learn how to switch from one setup
to another. The same procedure can also be used as the final steps of manipulator setup and
installation assuming the P-285 is mounted on or near your microscope.
1. Turn on the manipulator power; while still in the main menu press <1>. This selects
Setup 1 and allows for rapid positioning of the pipette in your microscopic field of view.
The upper left-hand corner of the display should now read “S⇒1”.
2. Press < OVE> to allow movement; remember that you cannot make movements unless
you see the movement screen displayed
3. Focus the microscope to a height slightly above your preparation.
4. Using the X and Y-axes, move the pipette tip to a position above the middle of your field
of view. In most cases, the first indication that the pipette is in the correct approximate
location is an out-of-focus shadow. You may need to work at a lower microscopic power to
visualize the tip.
5. Once you can see the shadow of the tip above your preparation, use the Z-axis control (top
ROE knob) to move the pipette down into focus. If you focused above your preparation,
you should not crash the pipette into your dish and/or preparation.

MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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If these operations are part of your initial installation, your manipulator may not be mounted
in a position to allow you to center the pipette tip in the microscope field of view. If this is
true, you will have to stop and adjust the mounting location of the manipulator and/or the
angle of approach of the pipette and/or the mounting of the headstage in the rotary dovetail.
After repositioning, you will be able to bring the pipette to the correct working location. See
the P-285 Reference anual for additional information on pipette positioning and Sutter
accessories to aid in pipette access to different microscopes.
NOTE: Once you can see the pipette in your field of view, you are ready for
micromanipulation.
1. Press <ESC> to get to the main menu
2. Press <4> to select Setup 4; the upper left hand corner of the display should now read
S⇒4.
3. Press < OVE> to return to the movement screen.
4. Rotate the knobs on the ROE and observe the precise control you have over the
movement of the pipette. If you wish to have even better control, press the
<COARSE/FINE> button on the ROE (see below)
Four factory-installed Setups are selected from the main menu by pressing the corresponding
number (1 through 4) from the P-285 keypad. Assuming you are in the movement screen
and you want to change the current Setup, the key sequence is as follows:
1. From the movement screen press <ESC> to access the main menu
2. Press the number key <1> through <4> corresponding to the Setup you wish to use.
The actual values for number of microsteps per ROE turn are as follows:
Table 3-1. icrosteps per ROE turn settings.
Setup
Number
icrosteps per
ROE turn
1 3694
2 5120
3 2560
4 512
3. Press < OVE> to return to the movement screen and begin moving at the precision
specified by the selected Setup.
The selected setup is maintained when the P-285 controller is turned off; you only need to
select a different setup when you wish to change precision of movement.
3.3 Changing inimal Resolution
In addition to controlling the number of microsteps per ROE turn, you can also set the
microstep size to one of two values (coarse=0.2µm or fine=0.04µm). You then have two
different amounts of movement (ultimately two different precisions for each selected Setup).
To change resolution from one value to the other, simply press the <COARSE/FINE> button