Sutter Instrument TRIO MPC-165 Series User manual

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Novato, CA 94949
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TRIO™MPC-165
Series
Dual 3-Axis Motorized Narrow-Format
Micromanipulator System
With Synthetic Fourth Diagonal Axis
Operation Manual
Rev. 2.67k2 (20201130) (FW v2.62)

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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(The picture on the cover page shows a TRIO MPC-100 ROE/controller and two TRIO MP-865M
micromanipulators (one right-handed and the other left-handed). The Sutter Instrument dPatch Headstages
shown mounted on the micromanipulators are not included in the TRIO MPC-165 Series system.)
Copyright © 2020 Sutter Instrument Company. All Rights Reserved.
TRIO™ is a trademark of Sutter Instrument Company.

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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DISCLAIMER
The TRIO MPC-165 series system consists of one or two electromechanical
micromanipulator devices and one TRIO MPC-100 ROE (Rotary Optical Encoder) with
integrated controller. The purpose of the system is for the manipulation at the micro level of
micropipettes and probes used in conjunction with a microscope. No other use is
recommended.
This instrument is designed for use in a laboratory environment. It is not intended nor
should it be used in human experimentation or applied to humans in any way. This is not a
medical device.
Unless otherwise indicated in this manual or by Sutter Instrument Technical Support for
reconfiguration, do not open or attempt to repair the instrument.
Do not allow an unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAFETY WARNINGS AND PRECAUTIONS
Electrical
Operate the TRIO MPC-100 using 110 – 240 VAC., 50-60 Hz line voltage. This instrument
is designed for use in a laboratory environment that has low electrical noise and
mechanical vibration. Surge suppression is always recommended
NOTE: There are no user-replaceable fuses in the TRIO MPC-100 system.
The TRIO MPC-100 system’s power supply consists of an external AC to DC
switching power adapter. If the external power adapter is damaged due to a mains over or
under voltage, it must be replaced.
GROUNDING/EARTHING: Proper grounding protects the ROE/controller
electronics, reduces/eliminates electromagnetic interference, and improves the safety of the
system operator. The ROE/controller provides a socket (labeled GROUND) that accepts a
banana plug attached to a suitably gauged insulated wire, the other end of which (alligator
clip) connects to a solid, proper ground.
Avoiding Electrical Shock and Fire-related Injury
Always use the grounded power cord provided to connect the system’s power adapter to a
grounded/earthed mains outlet (3-prong). This is required to protect you from injury if an
electrical hazard occurs.
Do not disassemble the system. Refer servicing to qualified personnel.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
Electromagnetic Interference
To comply with FDA and CE/EU electromagnetic immunity and interference standards; and
to reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided for interconnecting the electro-
mechanical devices and ROE/controller (refer to Technical Specifications for more details).

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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Operational
Failure to comply with any of the following precautions may damage this device.
This instrument is designed for operation in a laboratory environment (Pollution Degree I)
that is free from mechanical vibrations, electrical noise, and transients.
DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE
CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON.
Please allow at least 20 seconds after turning the unit off before disconnecting the
mechanical units. Failure to do so may result in damage to the electronics.
Operate this instrument only according to the instructions included in this manual.
Do not operate if there is any obvious damage to any part of the instrument.
Do not operate this instrument near flammable materials. The use of any hazardous
materials with this instrument is not recommended and, if undertaken, is done so at the
users’ own risk.
Do not operate if there is any obvious damage to any part of the instrument. Do not
attempt to operate the instrument with the MP-865/M electromechanical manipulator
shipping tape in place or severe motor damage may result. When transporting the
mechanical manipulator, be sure to reinstall the shipping tape (using masking tape or
equivalent only) to the original locations. Failure to do this may result in damage to the
motors.
Never touch any part of the micromanipulator electromechanical device while it is in
operation and moving. Doing so can result in physical injury (e.g., fingers can be caught
and pinched between the moving parts of the micromanipulator).
If the TRIO MPC-100 system is used in a microinjection environment, please observe
the following. As with most micromanipulation devices, sharp micropipettes can fly out of
their holder unexpectedly. Always take precautions to prevent this from happening. Never
loosen the micropipette holder chuck when the tubing is pressurized, and never point
micropipette holders at yourself or others. Always wear safety glasses when using sharp
glass micropipettes with pressure tubing.
Take care to ensure no cables pass close to the MP-865/M electromechanical
micromanipulator within the spherical movement limits of all its axes combined.
Other
Retain the original packaging for future transport of the instrument.
Sutter Instrument reserves the right to change specifications without prior notice.
Use of this instrument is for research purposes only.
Handling Micropipettes
Failure to comply with any of the following precautions may result in injury to the users
of this device as well as those working in the general area near the device.
The micropipettes used with this instrument are very sharp and relatively fragile. Avoid
contact with micropipette tips to prevent accidentally impaling oneself.
Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps”
container.

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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TABLE OF CONTENTS
DISCLAIMER .......................................................................................................................................3
SAFETY WARNINGS AND PRECAUTIONS....................................................................................3
Electrical ..................................................................................................................................................3
Avoiding Electrical Shock and Fire-related Injury..............................................................................3
Electromagnetic Interference ............................................................................................................3
Operational ..............................................................................................................................................4
Other.........................................................................................................................................................4
Handling Micropipettes..........................................................................................................................4
1. INTRODUCTION.............................................................................................................................9
1.1 Structure of the TRIO MPC-165 Series Documentation Package..............................................9
1.2 Components of the TRIO MPC-165 System..................................................................................9
1.3 Components of the TRIO MPC-165-2 System...............................................................................9
1.4 Overview ..........................................................................................................................................10
1.4.1 Features: TRIO MPC-100 Controller ....................................................................................11
1.4.2 Features: TRIO MP-865 3-Axis Narrow-Format Micromanipulator Electromechanical11
1.4.3 Description................................................................................................................................11
2. INSTALLATION ............................................................................................................................13
2.1 Mounting Instructions ...................................................................................................................13
2.1 Mounting MP-865/M to the MT-73 ..............................................................................................13
2.2 Setting Headstage/Pipette Angle and Pipette Exchange ...........................................................14
2.3 Headstage Mounting ......................................................................................................................15
2.4 Instructions Used in Special Installations Only..........................................................................16
2.4.1 Instructions for Changing Handedness.................................................................................16
2.5 Other Accessories............................................................................................................................16
2.6 Electrical Connections and Initial Operating Instructions........................................................16
2.7 ROE/Controller Rear Panel Controls and Configuration...........................................................17
2.7.1 Power Switch............................................................................................................................17
2.7.2 Configuration Switches ...........................................................................................................18
2.7.2.1 Switches 1, 2, 3 and 4........................................................................................................18
2.7.2.2 Switch 5: Y-Axis Lockout during Homing ......................................................................18
2.7.2.3 Switch 6: Calibration Homing on Power On..................................................................19
2.7.2.4 Switches 7 & 8 Reserved...................................................................................................19
2.7.2.5 Switch 9 Electromechanical Device Compatibility ........................................................19
2.7.2.6 Switch 10 Linear/nonlinear manual movement ............................................................19
3. OPERATIONS ................................................................................................................................21
3.1 Main Controls and Indicators on the ROE/Controller ...............................................................21
3.2 Display..............................................................................................................................................21
3.2.1 Initial Startup...........................................................................................................................21
3.3 Control Operations .........................................................................................................................22
3.3.1 Manipulator Select...................................................................................................................22
3.3.2 Maximum Positive Position Values: ......................................................................................22
3.3.3 Setting Position for HOME or WORK...................................................................................22
3.3.4 Setting the Angle of the Pipette/Headstage Holder .............................................................22
3.3.5 Operating the Virtual D Axis..................................................................................................23
3.3.6 Moving to the Home Position .................................................................................................23

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3.3.7 Moving to the Work Position ..................................................................................................24
3.3.8 Setting Absolute/Relative Coordinates Mode........................................................................24
3.3.9 Mode Indications ......................................................................................................................25
3.3.10 Speed Control and ROE Knob Movements (SPEED)........................................................25
3.3.11 Movement Knobs Disabling and Lock Mode ([SPEED]/LOCK) ......................................25
3.3.12 Pausing Home Movements (HOME (while moving to Home)) ........................................25
3.3.13 Pausing Work Movements (WORK (while moving to Work)) ..........................................25
3.3.14 Pulse Mode and Virtual D-Axis Movement (PULSE)........................................................25
3.4 Micropipette/Headstage Exchange ...............................................................................................25
4. EXTERNAL CONTROL ................................................................................................................27
4.1 General.............................................................................................................................................27
4.2 Virtual COM Port (VCP) Serial Port Settings.............................................................................27
4.3 Protocol and Handshaking ............................................................................................................27
4.4 Command Sequence Formatting ..................................................................................................28
4.5 Axis Position Command Parameters............................................................................................28
4.6 Microsteps and Microns (Micrometers)........................................................................................29
4.7 Travel Ranges and Bounds ............................................................................................................29
4.8 Travel Speed....................................................................................................................................30
4.9 Commands .......................................................................................................................................30
4.9.1 Get Active Device & Firmware Version (‘K’) Command.....................................................30
4.9.2 Change Active Device (‘I’) Command ....................................................................................30
4.9.3 Get Current Position and Angle (‘c’ or ‘C’) Command........................................................31
4.9.4 Move to Controller-Defined HOME Position (‘h’) Command.............................................32
4.9.5 Move to Controller-Defined WORK Position (‘w’) Command ............................................32
4.9.6 Move to Specified “Home” Position (‘H’) Command...........................................................32
4.9.7 Move to Specified “Work” Position (‘W’) Command............................................................32
4.9.8 Move in Straight Line to Specified Position at Specified Speed (‘S’) Command ..............33
4.9.9 Interrupt Straight-Line Move (‘^C’) Command .................................................................35
4.9.10 Move to Specified X-Axis Position (‘x’ or ‘X’) Command...................................................35
4.9.11 Move to Specified Y-Axis Position (‘y’ or ‘Y’) Command...................................................35
4.9.12 Move to Specified Z-Axis Position (‘z’ or ‘Z’) Command ...................................................36
4.9.13 Setting the Angle (‘A’) Command ........................................................................................36
4.9.14 Recalibrate (‘R’) Command...................................................................................................36
4.9.15 Command Notes.....................................................................................................................37
5. MAINTENANCE............................................................................................................................41
6. RECONFIGURATION...................................................................................................................42
6.1 Changing the Rotary Knob Functions on the ROE/Controller .................................................42
APPENDIX A. LIMITED WARRANTY............................................................................................43
APPENDIX B. ACCESSORIES .........................................................................................................44
APPENDIX C. TECHNICAL SPECIFICATIONS ...........................................................................45
APPENDIX D. QUICK REFERENCE..............................................................................................47
D.1. Manual Operation.........................................................................................................................47
D.2. Configuration.................................................................................................................................47
D.3. External Control ...........................................................................................................................48
INDEX .................................................................................................................................................55

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TABLE OF FIGURES
Figure 1-1. The TRIO MPC-165 system ................................................................................................ 10
Figure 1-2. The TRIO MPC-165-2 system............................................................................................. 10
Figure 2-1. Angled view of the MP-865/M showing pipette holder and angle control, and directions
of all three axes. ...................................................................................................................... 13
Figure 2-2. MT-73 controls and adjustments. ....................................................................................... 14
Figure 2-3. Location of screw to loosen for changing pipette angle. ...................................................15
Figure 2-4. Headstage mounting. ...........................................................................................................15
Figure 2-5. Using a dovetail for headstage mounting........................................................................... 16
Figure 2-6. Rear of TRIO MPC-100 ROE/Controller cabinet..............................................................17
Figure 2-7. Configuration switches on rear of TRIO MPC-100 ROE/Controller unit (switch
positions shown are factory defaults)...................................................................................18
Figure 3-1. LCD Display showing startup screen. ................................................................................21
Figure 3-2. Startup screen .......................................................................................................................21
Figure 3-3. Device A factory default startup (Home) position after calibration................................ 21
Figure 3-4. Device B factory default startup (Home) position after calibration................................22
Figure 3-5. Maximum positive values.....................................................................................................22
Figure 3-6. Moving to Home position (screen is amber while moving) .............................................. 23
Figure 3-7. Factory default Home position............................................................................................ 23
Figure 3-8. Example Home position defined and saved .......................................................................23
Figure 3-9. Example Work position........................................................................................................24
Figure 3-10. Relative mode......................................................................................................................24
Figure 3-11. Relative mode......................................................................................................................24
Figure 3-12. Absolute mode..................................................................................................................... 24
Figure 3-13. Changing the MP-865/M micropipette/headstage mount and angle. ...........................26
Figure 6-1. Locations of the axis connectors inside the ROE/Controller ...........................................42
TABLE OF TABLES
Table 2-1. Configuration Switches 1 - 4: Configuring the direction of each axis............................... 18
Table 2-2. Configuration Switch 5: Configuring the Homing Y-Movement Lock Out. ....................18
Table 2-3. Configuration Switch 6: Configuring the Sensor Test. ......................................................19
Table 2-4. Configuration Switches 7 & 8: Reserved.............................................................................. 19
Table 2-5. Configuration Switch 9: Electromechanical device compatibility..................................... 19
Table 2-6. Configuration Switch 10: Linear/nonlinear manual movement. ...................................... 19
Table 3-1. Maximum positive position value of each axis ....................................................................22
Table 3-2. Screen colors and modes........................................................................................................ 25

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Table 4-1. USB-VCP interface serial port settings. ..............................................................................27
Table 4-2. Microns/microsteps conversion.............................................................................................29
Table 4-3. Ranges and bounds................................................................................................................. 29
Table 4-4. Travel speeds........................................................................................................................... 30
Table 4-5. Get Active Device & Firmware Version (‘K’) command. ...................................................30
Table 4-6. Change active device (‘I’) command.....................................................................................31
Table 4-7. Get Current Position and Angle (‘c’ or ‘C’) command. ......................................................31
Table 4-8. Move to controller-defined HOME position (‘h’) command. ............................................. 32
Table 4-9. Move to controller -defined WORK position (‘w’) command............................................. 32
Table 4-10. Move to specified “Home” position (‘H’) command. ........................................................32
Table 4-11. Move to specified “Work” position (‘W’) command.......................................................... 33
Table 4-12. Straight-line move to specified position (‘S’) command...................................................33
Table 4-13. Straight-Line Move ‘S’ Command Speeds for MP-865/M-based configuration. ........... 34
Table 4-14. Straight-Line Move ‘S’ Command Speeds for MP-285/M-based configuration. ........... 34
Table 4-15. Interrupt a straight-line move in progress (‘^C’) command..........................................35
Table 4-16. Move to specified X-axis position (‘x’ or ‘X’) command.................................................... 35
Table 4-17. Move to specified Y-axis position (‘y’ or ‘Y’) command.................................................... 36
Table 4-18. Move to specified Z-axis position (‘z’) command............................................................... 36
Table 4-19. Set the angle (‘A’) command. ..............................................................................................36
Table 4-20. Recalibrate (‘R’) command..................................................................................................37
Table 4-21. Straight-Line Move ‘S’ Command Speeds for MP-865/M based configuration.............40
Table 4-22. Straight-Line Move ‘S’ Command Speeds for MP-285/M based configuration.............40
Table C-1. TRIO MPC-100 cables and receptacles/connectors............................................................ 46
Table D-1. Configuration Switches 1 – 10.............................................................................................. 47
Table D-2. USB-VCP interface serial port settings...............................................................................48
Table D-3. Microns/microsteps conversion............................................................................................49
Table D-4. Ranges and bounds................................................................................................................49
Table D-5. Travel speeds.......................................................................................................................... 49
Table D-6. TRIO MPC-100 external control commands...................................................................... 50
Table D-7. Straight-Line Move ‘S’ Command Speeds for MP-865/M-based configuration.............. 54
Table D-8. Straight-Line Move ‘S’ Command Speeds for MP-285/M-based configuration..............54