Sutter Instrument TRIO MPC-165 Series User manual

One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax: [phone] Email: [e-mail]
TRIO™MPC-165
Series
Dual 3-Axis Motorized Narrow-Format
Micromanipulator System
With Synthetic Fourth Diagonal Axis
Operation Manual
Rev. 2.67k2 (20201130) (FW v2.62)

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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(The picture on the cover page shows a TRIO MPC-100 ROE/controller and two TRIO MP-865M
micromanipulators (one right-handed and the other left-handed). The Sutter Instrument dPatch Headstages
shown mounted on the micromanipulators are not included in the TRIO MPC-165 Series system.)
Copyright © 2020 Sutter Instrument Company. All Rights Reserved.
TRIO™ is a trademark of Sutter Instrument Company.

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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DISCLAIMER
The TRIO MPC-165 series system consists of one or two electromechanical
micromanipulator devices and one TRIO MPC-100 ROE (Rotary Optical Encoder) with
integrated controller. The purpose of the system is for the manipulation at the micro level of
micropipettes and probes used in conjunction with a microscope. No other use is
recommended.
This instrument is designed for use in a laboratory environment. It is not intended nor
should it be used in human experimentation or applied to humans in any way. This is not a
medical device.
Unless otherwise indicated in this manual or by Sutter Instrument Technical Support for
reconfiguration, do not open or attempt to repair the instrument.
Do not allow an unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAFETY WARNINGS AND PRECAUTIONS
Electrical
Operate the TRIO MPC-100 using 110 – 240 VAC., 50-60 Hz line voltage. This instrument
is designed for use in a laboratory environment that has low electrical noise and
mechanical vibration. Surge suppression is always recommended
NOTE: There are no user-replaceable fuses in the TRIO MPC-100 system.
The TRIO MPC-100 system’s power supply consists of an external AC to DC
switching power adapter. If the external power adapter is damaged due to a mains over or
under voltage, it must be replaced.
GROUNDING/EARTHING: Proper grounding protects the ROE/controller
electronics, reduces/eliminates electromagnetic interference, and improves the safety of the
system operator. The ROE/controller provides a socket (labeled GROUND) that accepts a
banana plug attached to a suitably gauged insulated wire, the other end of which (alligator
clip) connects to a solid, proper ground.
Avoiding Electrical Shock and Fire-related Injury
Always use the grounded power cord provided to connect the system’s power adapter to a
grounded/earthed mains outlet (3-prong). This is required to protect you from injury if an
electrical hazard occurs.
Do not disassemble the system. Refer servicing to qualified personnel.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
Electromagnetic Interference
To comply with FDA and CE/EU electromagnetic immunity and interference standards; and
to reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided for interconnecting the electro-
mechanical devices and ROE/controller (refer to Technical Specifications for more details).

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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Operational
Failure to comply with any of the following precautions may damage this device.
This instrument is designed for operation in a laboratory environment (Pollution Degree I)
that is free from mechanical vibrations, electrical noise, and transients.
DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE
CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON.
Please allow at least 20 seconds after turning the unit off before disconnecting the
mechanical units. Failure to do so may result in damage to the electronics.
Operate this instrument only according to the instructions included in this manual.
Do not operate if there is any obvious damage to any part of the instrument.
Do not operate this instrument near flammable materials. The use of any hazardous
materials with this instrument is not recommended and, if undertaken, is done so at the
users’ own risk.
Do not operate if there is any obvious damage to any part of the instrument. Do not
attempt to operate the instrument with the MP-865/M electromechanical manipulator
shipping tape in place or severe motor damage may result. When transporting the
mechanical manipulator, be sure to reinstall the shipping tape (using masking tape or
equivalent only) to the original locations. Failure to do this may result in damage to the
motors.
Never touch any part of the micromanipulator electromechanical device while it is in
operation and moving. Doing so can result in physical injury (e.g., fingers can be caught
and pinched between the moving parts of the micromanipulator).
If the TRIO MPC-100 system is used in a microinjection environment, please observe
the following. As with most micromanipulation devices, sharp micropipettes can fly out of
their holder unexpectedly. Always take precautions to prevent this from happening. Never
loosen the micropipette holder chuck when the tubing is pressurized, and never point
micropipette holders at yourself or others. Always wear safety glasses when using sharp
glass micropipettes with pressure tubing.
Take care to ensure no cables pass close to the MP-865/M electromechanical
micromanipulator within the spherical movement limits of all its axes combined.
Other
Retain the original packaging for future transport of the instrument.
Sutter Instrument reserves the right to change specifications without prior notice.
Use of this instrument is for research purposes only.
Handling Micropipettes
Failure to comply with any of the following precautions may result in injury to the users
of this device as well as those working in the general area near the device.
The micropipettes used with this instrument are very sharp and relatively fragile. Avoid
contact with micropipette tips to prevent accidentally impaling oneself.
Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps”
container.

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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TABLE OF CONTENTS
DISCLAIMER .......................................................................................................................................3
SAFETY WARNINGS AND PRECAUTIONS....................................................................................3
Electrical ..................................................................................................................................................3
Avoiding Electrical Shock and Fire-related Injury..............................................................................3
Electromagnetic Interference ............................................................................................................3
Operational ..............................................................................................................................................4
Other.........................................................................................................................................................4
Handling Micropipettes..........................................................................................................................4
1. INTRODUCTION.............................................................................................................................9
1.1 Structure of the TRIO MPC-165 Series Documentation Package..............................................9
1.2 Components of the TRIO MPC-165 System..................................................................................9
1.3 Components of the TRIO MPC-165-2 System...............................................................................9
1.4 Overview ..........................................................................................................................................10
1.4.1 Features: TRIO MPC-100 Controller ....................................................................................11
1.4.2 Features: TRIO MP-865 3-Axis Narrow-Format Micromanipulator Electromechanical11
1.4.3 Description................................................................................................................................11
2. INSTALLATION ............................................................................................................................13
2.1 Mounting Instructions ...................................................................................................................13
2.1 Mounting MP-865/M to the MT-73 ..............................................................................................13
2.2 Setting Headstage/Pipette Angle and Pipette Exchange ...........................................................14
2.3 Headstage Mounting ......................................................................................................................15
2.4 Instructions Used in Special Installations Only..........................................................................16
2.4.1 Instructions for Changing Handedness.................................................................................16
2.5 Other Accessories............................................................................................................................16
2.6 Electrical Connections and Initial Operating Instructions........................................................16
2.7 ROE/Controller Rear Panel Controls and Configuration...........................................................17
2.7.1 Power Switch............................................................................................................................17
2.7.2 Configuration Switches ...........................................................................................................18
2.7.2.1 Switches 1, 2, 3 and 4........................................................................................................18
2.7.2.2 Switch 5: Y-Axis Lockout during Homing ......................................................................18
2.7.2.3 Switch 6: Calibration Homing on Power On..................................................................19
2.7.2.4 Switches 7 & 8 Reserved...................................................................................................19
2.7.2.5 Switch 9 Electromechanical Device Compatibility ........................................................19
2.7.2.6 Switch 10 Linear/nonlinear manual movement ............................................................19
3. OPERATIONS ................................................................................................................................21
3.1 Main Controls and Indicators on the ROE/Controller ...............................................................21
3.2 Display..............................................................................................................................................21
3.2.1 Initial Startup...........................................................................................................................21
3.3 Control Operations .........................................................................................................................22
3.3.1 Manipulator Select...................................................................................................................22
3.3.2 Maximum Positive Position Values: ......................................................................................22
3.3.3 Setting Position for HOME or WORK...................................................................................22
3.3.4 Setting the Angle of the Pipette/Headstage Holder .............................................................22
3.3.5 Operating the Virtual D Axis..................................................................................................23
3.3.6 Moving to the Home Position .................................................................................................23

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3.3.7 Moving to the Work Position ..................................................................................................24
3.3.8 Setting Absolute/Relative Coordinates Mode........................................................................24
3.3.9 Mode Indications ......................................................................................................................25
3.3.10 Speed Control and ROE Knob Movements (SPEED)........................................................25
3.3.11 Movement Knobs Disabling and Lock Mode ([SPEED]/LOCK) ......................................25
3.3.12 Pausing Home Movements (HOME (while moving to Home)) ........................................25
3.3.13 Pausing Work Movements (WORK (while moving to Work)) ..........................................25
3.3.14 Pulse Mode and Virtual D-Axis Movement (PULSE)........................................................25
3.4 Micropipette/Headstage Exchange ...............................................................................................25
4. EXTERNAL CONTROL ................................................................................................................27
4.1 General.............................................................................................................................................27
4.2 Virtual COM Port (VCP) Serial Port Settings.............................................................................27
4.3 Protocol and Handshaking ............................................................................................................27
4.4 Command Sequence Formatting ..................................................................................................28
4.5 Axis Position Command Parameters............................................................................................28
4.6 Microsteps and Microns (Micrometers)........................................................................................29
4.7 Travel Ranges and Bounds ............................................................................................................29
4.8 Travel Speed....................................................................................................................................30
4.9 Commands .......................................................................................................................................30
4.9.1 Get Active Device & Firmware Version (‘K’) Command.....................................................30
4.9.2 Change Active Device (‘I’) Command ....................................................................................30
4.9.3 Get Current Position and Angle (‘c’ or ‘C’) Command........................................................31
4.9.4 Move to Controller-Defined HOME Position (‘h’) Command.............................................32
4.9.5 Move to Controller-Defined WORK Position (‘w’) Command ............................................32
4.9.6 Move to Specified “Home” Position (‘H’) Command...........................................................32
4.9.7 Move to Specified “Work” Position (‘W’) Command............................................................32
4.9.8 Move in Straight Line to Specified Position at Specified Speed (‘S’) Command ..............33
4.9.9 Interrupt Straight-Line Move (‘^C’) Command .................................................................35
4.9.10 Move to Specified X-Axis Position (‘x’ or ‘X’) Command...................................................35
4.9.11 Move to Specified Y-Axis Position (‘y’ or ‘Y’) Command...................................................35
4.9.12 Move to Specified Z-Axis Position (‘z’ or ‘Z’) Command ...................................................36
4.9.13 Setting the Angle (‘A’) Command ........................................................................................36
4.9.14 Recalibrate (‘R’) Command...................................................................................................36
4.9.15 Command Notes.....................................................................................................................37
5. MAINTENANCE............................................................................................................................41
6. RECONFIGURATION...................................................................................................................42
6.1 Changing the Rotary Knob Functions on the ROE/Controller .................................................42
APPENDIX A. LIMITED WARRANTY............................................................................................43
APPENDIX B. ACCESSORIES .........................................................................................................44
APPENDIX C. TECHNICAL SPECIFICATIONS ...........................................................................45
APPENDIX D. QUICK REFERENCE..............................................................................................47
D.1. Manual Operation.........................................................................................................................47
D.2. Configuration.................................................................................................................................47
D.3. External Control ...........................................................................................................................48
INDEX .................................................................................................................................................55

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TABLE OF FIGURES
Figure 1-1. The TRIO MPC-165 system ................................................................................................ 10
Figure 1-2. The TRIO MPC-165-2 system............................................................................................. 10
Figure 2-1. Angled view of the MP-865/M showing pipette holder and angle control, and directions
of all three axes. ...................................................................................................................... 13
Figure 2-2. MT-73 controls and adjustments. ....................................................................................... 14
Figure 2-3. Location of screw to loosen for changing pipette angle. ...................................................15
Figure 2-4. Headstage mounting. ...........................................................................................................15
Figure 2-5. Using a dovetail for headstage mounting........................................................................... 16
Figure 2-6. Rear of TRIO MPC-100 ROE/Controller cabinet..............................................................17
Figure 2-7. Configuration switches on rear of TRIO MPC-100 ROE/Controller unit (switch
positions shown are factory defaults)...................................................................................18
Figure 3-1. LCD Display showing startup screen. ................................................................................21
Figure 3-2. Startup screen .......................................................................................................................21
Figure 3-3. Device A factory default startup (Home) position after calibration................................ 21
Figure 3-4. Device B factory default startup (Home) position after calibration................................22
Figure 3-5. Maximum positive values.....................................................................................................22
Figure 3-6. Moving to Home position (screen is amber while moving) .............................................. 23
Figure 3-7. Factory default Home position............................................................................................ 23
Figure 3-8. Example Home position defined and saved .......................................................................23
Figure 3-9. Example Work position........................................................................................................24
Figure 3-10. Relative mode......................................................................................................................24
Figure 3-11. Relative mode......................................................................................................................24
Figure 3-12. Absolute mode..................................................................................................................... 24
Figure 3-13. Changing the MP-865/M micropipette/headstage mount and angle. ...........................26
Figure 6-1. Locations of the axis connectors inside the ROE/Controller ...........................................42
TABLE OF TABLES
Table 2-1. Configuration Switches 1 - 4: Configuring the direction of each axis............................... 18
Table 2-2. Configuration Switch 5: Configuring the Homing Y-Movement Lock Out. ....................18
Table 2-3. Configuration Switch 6: Configuring the Sensor Test. ......................................................19
Table 2-4. Configuration Switches 7 & 8: Reserved.............................................................................. 19
Table 2-5. Configuration Switch 9: Electromechanical device compatibility..................................... 19
Table 2-6. Configuration Switch 10: Linear/nonlinear manual movement. ...................................... 19
Table 3-1. Maximum positive position value of each axis ....................................................................22
Table 3-2. Screen colors and modes........................................................................................................ 25

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Table 4-1. USB-VCP interface serial port settings. ..............................................................................27
Table 4-2. Microns/microsteps conversion.............................................................................................29
Table 4-3. Ranges and bounds................................................................................................................. 29
Table 4-4. Travel speeds........................................................................................................................... 30
Table 4-5. Get Active Device & Firmware Version (‘K’) command. ...................................................30
Table 4-6. Change active device (‘I’) command.....................................................................................31
Table 4-7. Get Current Position and Angle (‘c’ or ‘C’) command. ......................................................31
Table 4-8. Move to controller-defined HOME position (‘h’) command. ............................................. 32
Table 4-9. Move to controller -defined WORK position (‘w’) command............................................. 32
Table 4-10. Move to specified “Home” position (‘H’) command. ........................................................32
Table 4-11. Move to specified “Work” position (‘W’) command.......................................................... 33
Table 4-12. Straight-line move to specified position (‘S’) command...................................................33
Table 4-13. Straight-Line Move ‘S’ Command Speeds for MP-865/M-based configuration. ........... 34
Table 4-14. Straight-Line Move ‘S’ Command Speeds for MP-285/M-based configuration. ........... 34
Table 4-15. Interrupt a straight-line move in progress (‘^C’) command..........................................35
Table 4-16. Move to specified X-axis position (‘x’ or ‘X’) command.................................................... 35
Table 4-17. Move to specified Y-axis position (‘y’ or ‘Y’) command.................................................... 36
Table 4-18. Move to specified Z-axis position (‘z’) command............................................................... 36
Table 4-19. Set the angle (‘A’) command. ..............................................................................................36
Table 4-20. Recalibrate (‘R’) command..................................................................................................37
Table 4-21. Straight-Line Move ‘S’ Command Speeds for MP-865/M based configuration.............40
Table 4-22. Straight-Line Move ‘S’ Command Speeds for MP-285/M based configuration.............40
Table C-1. TRIO MPC-100 cables and receptacles/connectors............................................................ 46
Table D-1. Configuration Switches 1 – 10.............................................................................................. 47
Table D-2. USB-VCP interface serial port settings...............................................................................48
Table D-3. Microns/microsteps conversion............................................................................................49
Table D-4. Ranges and bounds................................................................................................................49
Table D-5. Travel speeds.......................................................................................................................... 49
Table D-6. TRIO MPC-100 external control commands...................................................................... 50
Table D-7. Straight-Line Move ‘S’ Command Speeds for MP-865/M-based configuration.............. 54
Table D-8. Straight-Line Move ‘S’ Command Speeds for MP-285/M-based configuration..............54

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1. INTRODUCTION
1.1 Structure of the TRIO MPC-165 Series Documentation Package
The TRIO MPC-165 series is a single or dual 3-axis motorized micromanipulator system
comprised of the TRIO MPC-100 ROE/controller, a power adapter, and one or two MP-865/M
stepper-motor-based electromechanical micromanipulators. This manual consists of five
parts: This chapter, Introduction, which provides an overview and general description of the
TRIO MPC-165 series system; Chapter 2, Installation, which describes how to install, set up,
and configure all components of the system; Chapter 3, Operations, which describes how to
operate the TRIO MPC-100 ROE/controller; Chapter 4, External Control, describes how to
control the system by computer; Chapter 5, Maintenance, describes how to perform routine
and other maintenance; and Chapter 6, Reconfiguration, describes the reconfiguration
possibilities of the TRIO MPC-100 ROE/controller.
1.2 Components of the TRIO MPC-165 System
Carefully remove all components from the shipping container. In addition to this manual, the
following should be included:
TRIO MPC-100 ROE Rotary Optical Encoder input device with built-in controller and
external power adapter.
MP-865/M electromechanical micromanipulator
25-pin DSUB cable (connects the ROE/controller to the micromanipulator).
Power adapter
Power adapter AC mains cable appropriate for your location
Ground/Earth cable
USB Cable
1.3 Components of the TRIO MPC-165-2 System
Same components as the TRIO MPC-165 system, with an additional MP-865/M
electromechanical manipulator and 25-pin DSUB cable.

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1.4 Overview
Figure 1-1. The TRIO MPC-165 system
Figure 1-2. The TRIO MPC-165-2 system
TRIO MPC-100 ROE/CONTROLLER
MP-865/M ELECTROMECHANICAL
MICROMANIPULATOR (E.G., A, RIGHT-HANDED)
MP-865/M ELECTROMECHANICAL
MICROMANIPULATOR (E.G., B,
LEFT-HANDED)
TRIO MPC-100
RO
E/CONTROLLER
MP-865/M ELECTROMECHANICAL
MICRO-MANIPULATOR (E.G., A,
RIGHT-HANDED)

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1.4.1 Features: TRIO MPC-100 Controller
•Controls one or two micromanipulators
•Three independent axes (X, Y, and Z) each with a virtual fourth axis (D) for coaxial
pipette movement utilizing a tangent function factoring the holder’s angle and the X and
Z axes.
•Sub-micron (less than 100 nm) resolution
•User selectable angle from 0 - 90 degrees via ROE input
•Fast movement with a top speed of 3 mm/sec (while homing)
•Digital display indicates coordinates in relative or absolute of active manipulator
•User-friendly, fanless compact controller with ROE preserves bench space
•Push button control of multiple functions – work, home, lock, pulse, relative mode, and
angle set
•Robotic home- and work-position moves for easy automated pipette exchange
•Configuration DIP switches to select each axis’ control knob rotation directionality
•USB interface for external computer control
1.4.2 Features: TRIO MP-865 3-Axis Narrow-Format Micromanipulator Electromechanical
•Mechanically robust construction for high stability
•Precision cross-roller bearings
•Three independent axes – 50 mm travel in X, 12.5 mm in Y, and 25 mm in Z
•Carries up to a kilogram
•Suited for
in vitro and in vivo electrophysiological recording
•Universal mounting system for headstage or pipette holder
•Optional MT-73 narrow stand with linear slide and built in rotating base
1.4.3 Description
Based on the original Sutter Instrument TRIO™ motorized 3-axis micromanipulator, the
TRIO MPC-100 expands the TRIO’s capabilities by supporting up to two micromanipulators
connected to a single ROE (Rotary Optical Encoder) and controller. A switch is provided on
the front part of the controller for selecting which of the two manipulators is active for
manual control. The X, Y, and Z axes provide 50, 12.5, and 25 mm range of motion (MP-
865/M). D-axis movement virtually uses a tangent function of the chosen angle of the holder
and simultaneously moving X and Z. The ROE controller has a digital display and keys for
Home, Work, Pulse, Angle Set, Speed, Lock (Quiet) Mode, and Relative positioning. The
compact, intuitive controller takes up minimal bench space, is fan-free, and easy to use.
While the axes provide X, Y, and Z orthogonal motion typical of most motorized
manipulators, a synthetic diagonal axis is available with the TRIO MPC-100 allowing one to
move the electrode coaxially using X and Z at the exact desired angle of approach using a
separate “D” control knob.

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The TRIO MPC-100’s ROE provides fine control of electrode position and the rate of rotation
of ROE dials for each axis determines the speed of travel. The finest step size is less than
100nm. Five conveniently located buttons on the ROE provide control of all the basic
functions you will need in normal operation (Work, Home, Speed/Lock, Relative, and
Pulse/Angle).
Press and hold WORK (for 3 seconds) to quickly store a work position, tap HOME to move all
axes to an initial location that is useful for changing electrodes, or press and hold the HOME
button (for 3 seconds) to memorize a new HOME position.
When ready to record data, suppress the motor drive electronics by pressing and holding for
3 seconds the SPEED/LOCK button. In the LOCK mode, the display turns red and ROE
input is locked out to avoid any accidental motion.
Pressing and holding the RELATIVE button for three seconds at any location causes the
display coordinates to change to all zeroes. When activating relative mode, the display turns
blue.
To return to viewing the absolute coordinates, tap the RELATIVE button to toggle back to
absolute positioning mode.
Finally, tapping the PULSE button causes a 3μm advance in the diagonal. This rapid burst of
forward motion can assist in sharp electrode cell penetration. Pressing and holding the
PULSE button for 3 seconds allows for entering the angle to which the electrode/headstage is
physically set so that X & Z coaxial (diagonal) movement is made at the correct angle.
All the electronics, except for a small power supply, are housed within the TRIO MPC-100
ROE and no separate controller or computer is required.
External computer control of the TRIO MPC-100 is possible via the USB connector mounted
on the controller/ROE’s rear panel. The controller’s internal software is programmed with a
defined set of commands allowing for a wide range of micromanipulator/stage movements as
programmed in software residing in an external computer connected via USB.
Designed with maximum flexibility in mind, a DIP switch on the controller changes the
directional movement of the ROE knobs to accommodate the preference of the user. A Y-axis
lockout function (accessible by DIP switch) is also available, allowing X/Z-only axial
movement during HOME and WORK repositioning. The TRIO comes standard with a
universal mounting system suitable for the most popular headstages or pipette holders.
The new MP-865/M micromanipulator features construction based on the TRIO-845, but
with the addition of precision cross-roller bearings for even tighter tolerances, and a 25pin
connector for future compatibility with our other controllers. The MP-865/M is a highly
stable 3-axis manipulator with 50 mm of travel on X axis, 12.5 mm on Y, and 25 mm on Z.
The TRIO MPC-100 controller gives the MP-865/M a synthetic fourth axis that can be set in
software to any angle between 0 and 90 degrees for diagonal movement. Based on a lead-
screw design with a smaller overall size and footprint than most manipulators, the MP-865/M
is ideal for applications that require 2 pipettes in one setup or for setups where space is
limited.

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2. INSTALLATION
When installing the TRIO MPC-100 system for the first time, it is recommended that the
components of the system be installed in the following order: MP-865/M electromechanical
micromanipulator first, followed by the TRIO MPC-100/E ROE/Controller.
IMPORTANT
Once the TRIO MPC-165 series system has been unpacked, remove the shipping tape from
the various locations on each MP-865/M series electromechanical micromanipulator. The
shipping tape must be removed before operating the TRIO MPC-100 system. If you need to
transport the MP-865/M series in the future, reapply 2 to 3-inch pieces of masking tape to the
same locations. Once the tape has been removed, handle the MP-865/M with care. The
mechanisms can be damaged if any of the axes are inadvertently moved without the tape in
place.
2.1 Mounting Instructions
This chapter describes how to mount the MP-865/M to a stand (normally, the top plate of an
MT-73, how to change pipettes and adjust pipette angle, and finally, the modular nature of
the mechanical.
Figure 2-1. Angled view of the MP-865/M showing pipette holder and angle control, and directions of all three
axes.
2.1 Mounting MP-865/M to the MT-73
The MP-865/M mounts to the top mounting plate of the MT-73 (or the now discontinued MT-
72 LS). Gently place the MP-865/M onto the four mounting posts that are part of the
mounting plate (X265210) using four M3X0.5 hex head screws. Once placed evenly on the
mounting plate, tighten the miniature hex screws (two on each side) of the MP-865/M’s X-
axis slide using the supplied hex wrench.
X
Y
Z
Pipette holder and
angle control
MP-865/M

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Figure 2-2. MT-73 controls and adjustments.
Only the front pair of screws is visible in this figure. The adapter plate is normally shipped in
a small plastic bag also containing the necessary hardware.
The figure above shows the plate removed from the X-axis. Before attaching the adapter
plate to the MP-865/M, you need to decide where to position the manipulator on your stand.
The stand can be any flat surface carrying ¼-20 or 10-32 holes on one-inch centers (such as a
Sutter Instrument’s MT-series stands or MD series platforms). A good technique is to set the
manipulator on top of the adapter plate and slide it around on your stand until it is in a good
location. Then take the manipulator off and fix the adapter plate securely to the stand with
the ¼-20 or 10-32 hardware. Finally, align the holes on the bottom corners of the
manipulator with the four threaded holes on the corners of the adapter plate and attach the
manipulator using the 4 supplied M3X0.5 metric cap screws.
2.2 Setting Headstage/Pipette Angle and Pipette Exchange
Mounted on the front of the Z-axis of the manipulator is the rotary dovetail. The rotary
dovetail is used to hold various electrophysiological headstages and/or micro tools at defined
angles.
The angle of the rotary dovetail is adjusted by loosening the hex set screw located on the
hinge side of the rotary dovetail (see figure below). You can set an angle using the knife-edge
on the dovetail and the scale on the faceplate. After choosing an angle, retighten the screw to
fix the angle.
Extended X-axis slide
locking lever
Extended X-axis slide
Adjustable
elevation
Mounting plate for the MP-865/M

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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Figure 2-3. Location of screw to loosen for changing pipette angle.
To change pipettes, squeeze the linear slide cantilever and pull the MP-865/M back along the
slide mounted on the MT-73 gantry to a position that works best for removing and replacing
pipettes and/or rods.
2.3 Headstage Mounting
Sutter Instrument IPA®and dPatch®headstages, Axon headstages 203B or CV-7, and the
Heka EPC-10 headstage have an integral dovetail that slides directly into the rotary dovetail
on the MP-865/M. The figure below shows an example of this type of headstage mounted in a
left-handed manipulator and in profile (on the right), the location of the Phillips-head screw
that secures the headstage dovetail in its mate on the manipulator.
Figure 2-4. Headstage mounting.
Older Axon and Heka headstages mount using the 4-inch dovetail (X285204) and a plastic
plate. A typical headstage of this type is shown mounted in a right-handed MP-865/M (right
panel). The plastic plate used with the 4-inch dovetail is shown in the left panel of the figure
Hex set screw: Loosen
to change pipette angle
Sutter Instrument IPA
headstage

TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
16
and the holes are indicated to mount common headstages. Additional holes could be easily
added to accommodate less common headstage footprints.
Connection to 4 inch dovetail:
( 3 holes, D)
EPC7,8 & 9: 1-1/16" x 2-7/32"
(2 holes, A & B)
CV-4: 1-1/8" x 1-15/16"
(4 holes, B& C)
A
B
C
C
C
DDD
Figure 2-5. Using a dovetail for headstage mounting.
Rod mounted headstages and micro tools are accommodated by use of a rod clamp that fits
into the dovetail (not shown). All the headstage adapters and mounting hardware are
included with the manipulator and are shipped in a zip lock plastic bag.
2.4 Instructions Used in Special Installations Only
2.4.1 Instructions for Changing Handedness
It is possible to change handedness of the MP-865/M mechanical. If you wish to do this,
please contact Sutter Instrument for details. If you wish, Sutter can change the handedness
for you. We will charge for shipping only.
2.5 Other Accessories
One or more accessories may have been ordered and received for mounting the MP-865/M
and/or modifying the headstage mount to the manipulator (i.e., rotating base, microscope
stage mount, gantry, dovetail extension, etc.). Setup of these accessories is normally covered
in documentation accompanying the accessory.
2.6 Electrical Connections and Initial Operating Instructions
Initially, you may want to simply connect the MP-865/M micromanipulator and the
ROE/Controller together and try some gross movements in order to get a feel for the controls
and how to make simple movements. It is perfectly acceptable to set the manipulators in the
middle of a bench top, make all electrical connections and then observe each unit’s
movement by eye.
CAUTION: Unless the MP-865/M micromanipulator electromechanical baseplate is
firmly bolted down to a breadboard or solidly to a firm surface, the manipulator is likely to
tip over when fully extending all of its axes, especially if it is loaded with a headstage that
extends beyond the manipulator’s current center of gravity.

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Upon deciding to directly install the TRIO MPC-100 system in your rig, it is useful to follow
the initial setup procedure to learn how to move the units to allow easy access to the
mounting screws.
1. With the power switch on the back of the ROE in the OFF (0) position, connect the power
adapter’s 24VDC cable to the POWER receptacle.
Figure 2-6. Rear of TRIO MPC-100 ROE/Controller cabinet
2. With the power OFF (rear panel switch in the “0” position), connect a well-
grounded/earthed wire to the GROUND banana plug receptacle.
3. With the power OFF, connect the male end of the DB -25 cable to either MANIPULATOR
connector on the ROE, the other end of which is connected to the MP-865/M
micromanipulator electromechanical stage.(See cautionary note below.)
4. Verify that the ten switches associated with the appropriate MANIPULATOR (A or B) on
the rear of the ROE are set as desired.
5. Power up the system by moving the power switch on the rear of the ROE to the “1”
position.
*CAUTION: NEVER CONNECT OR DISCONNECT THE ROE/CONTROLLER
FROM EITHER ELECTROMECHANICAL MANIPULATOR/STAGE WHILE THE POWER
IS ON!
2.7 ROE/Controller Rear Panel Controls and Configuration
2.7.1 Power Switch
The power switch for the TRIO MPC-100 system is located on the rear panel of the
ROE/controller. At power up, the microprocessor in the ROE/controller scans the attached
equipment and configures the system accordingly.
CAUTION: When changing the configuration switches described in the following paragraphs,
make sure that controller’s power switch is turned off.
Power
Switch
USB
Device
Receptacle
Ground/Earth
P
ost
Manipulator A
cab
le connector
1
0
Power
Input
Socket
Manipulator B
configuration
swi
tches
Manipulator B
cable connector
Manipulator A
conf
iguration
switches

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2.7.2 Configuration Switches
Each manipulator channel (A and B) has its own bank of configuration switches. Switch bank
A affects only MANIPULATOR A, and switch bank B likewise for MANIPULATOR B.
Figure 2-7. Configuration switches on rear of TRIO MPC-100 ROE/Controller unit (switch positions shown are
factory defaults).
2.7.2.1 Switches 1, 2, 3 and 4
These switches set the directionality for each of the four axes.
Table 2-1. Configuration Switches 1 - 4: Configuring the direction of each axis.
Switch #
Definition
State
Setting
Position
1
X-Axis Knob Directionality for
Forward (+) Movement
Clockwise
OFF*
UP*
Counterclockwise
ON
DOWN
2
Y-Axis Knob Directionality for
Forward (+) Movement
Clockwise
OFF*
UP*
Counterclockwise
ON
DOWN
3
Z-Axis Knob Directionality for
Forward (+) Movement
Clockwise
OFF*
UP*
Counterclockwise
ON
DOWN
4
D-Axis Knob Directionality for
Forward (+) Movement
Clockwise
OFF*
UP*
Counterclockwise
ON
DOWN
* Factory default (typical setting for right-hand-mounted manipulator).
2.7.2.2 Switch 5: Y-Axis Lockout during Homing
Table 2-2. Configuration Switch 5: Configuring the Homing Y-Movement Lock Out.
Switch #
Definition
State
Setting
Position
5
Y-Movement lock out
during homing
(pressing HOME or
WORK)
Enabled: Locked
(no movement while X & Z are homing) OFF UP
Disabled: Not locked
(Y moves along with X & Z when homing)
ON*
DOWN*
* Factory default (recommended normal operation setting)
1 2 3 4 5 6 7 8 9 10