Sutter Instrument Lambda DG-4Plus/USB User manual

One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax: [phone] Email: [e-mail]
Lambda DG-4Plus/USB
and
Lambda DG-5Plus/USB
Ultra-High-Speed Wavelength
Switching Xenon Arc Light Source
System
Operation Manual
Rev. 1.01b3 (20190607)

LAMBDA DG-4 & 5 PLUS/USB OPERATION MANUAL – REV. 1.01B3 (20190607)
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Copyright © 2019 Sutter Instrument Company. All Rights Reserved.
LAMBDA-10®and SmartShutter®are registered trademarks of Sutter Instrument
Company.

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DISCLAIMER
The ultra high speed wavelength switching xenon arc lamp Lambda DG-4PLUS/USB/DG-
5PLUS/USB system is designed for the specific use as a microscope illuminator and optical
wavelength switcher, and no other use is recommended.
This instrument should only be used in a laboratory environment for use on animal tissues.
It is not intended for, nor should it be used in, human experimentation or applied to humans
in any way. This is not a medical device.
Do not open or attempt to repair the instrument without expressed and explicit instructions
from Sutter Instrument Company. Extreme heat and high voltages are present and could
cause injury.
Do not allow unauthorized and or untrained operatives to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instruments assumes
no implied or inferred liability for direct or consequential damages from this instrument if it
is operated or used in any way other than for which it is designed.
SAFETY WARNINGS AND PRECAUTIONS
Electrical
Operate the Lambda DG-4PLUS/USB and DG-5PLUS/USB using 100- 240 V AC, 50/60
Hz line voltage. This instrument is designed for connection to a standard laboratory
power outlet (Overvoltage Category II), and because it is a microprocessor--controlled
device, it should be accorded the same system wiring precautions as any 'computer type'
system. A surge protector and power regulator are recommended.
Fuse Replacement: Replace only with the same type and rating:
5 x 20 mm glass tube, time delay (medium), time lag, or “slow blow”)
Rating:
Xenon
Lamp
Power
Rating
Line Voltage: 100 – 240 VAC
Fuse
Rating
Manufacturer Examples
RoHS Compliant (Lead Free)?
Yes
No
300 Watts
T6.3A,
250V
Bussmann: S506-6.3-R
Littelfuse: 218 06.3P
Bussmann: GDC-6.3A
Littelfuse: 218 06.3
A spare fuse is located in the power input module. Please refer to the fuse-replacement
appendix for more details on fuse ratings and for instructions on how to change the fuse.
Avoiding Electrical Shock and Fire-related Injury
Always use the grounded power supply cord set provided to connect the unit to a
grounded outlet (3-prong). This is required to protect you from injury in the event that
an electrical hazard occurs.
Do not disassemble the unit. The only user serviceable parts are the line fuse, the xenon
bulb, and filters. The line fuse is accessible from the outside of the unit. The bulb and the

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filters are accessible via separate panels on the side of the unit. Bulb replacement and
filter installation/removal are covered in separate sections of the manual and should only
be attempted with the power cord disconnected.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
Electromagnetic Interference
To comply with FDA and CE electromagnetic immunity and interference standards; and to
reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided with the unit for the interconnection
of one or more filter wheels and/or shutters, host computer via serial RS-232 or parallel
interface, or other equipment via TTL connections (see the TECHNICAL SPECIFICATIONS
appendix for more details).
Operational
Operate only in a location where there is a free flow of fresh air on all sides.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.
Do not operate the lamp in an orientation where the output of the CERMAX lamp faces
within 45°of straight upward. To do so may cause arc instability and the possibility of
damage to the front window of the CERMAX lamp. Note that the lamp output is towards
the heat sink mounted on the side of the cabinet.
Avoiding Physical Injury while Powered Up and Emitting Light
WARNING: DO NOT LOOK DIRECTLY INTO THE LIGHT GUIDE! The output
of the light aperture or the light guide should be directed into the microscope using the
appropriate adapters, directed away from anyone’s eyes, and not directed toward any
reflective surface.
INFRARED RADIATION: The infrared radiation (and ultraviolet radiation)
generated by this lamp can cause significant skin burns and eye damage.
THE SMARTSHUTTER IS NOT A SAFETY SHUTTER: The Lambda DG-
4PLUS/USB and DG-5PLUS/USB equipped with a Sutter Instrument Smart Shutter. The
SmartShutter is not intended to be a ‘safety shutter.’ A safety shutter usually closes
automatically in the event of a power failure and is designed with the primary goal of
ensuring that it will not allow any unintended exposure. For laser safety applications, a
shutter is normally designed so that no single component failure allows an unintended
exposure to the laser beam. The SmartShutter is intended for use in the controlling of
light in scientific and industrial applications. The SmartShutter was designed for high
performance and durability, but without certain features that would be desirable in a
safety shutter application.
EXPLOSION: High internal pressure exists in any xenon arc lamp.

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HIGH VOLTAGE: High ignition voltages, which exist inside the cabinet, can be
lethal.
WARNING: OZONE: The UV-enhanced (“full spectrum”) version of the xenon arc
bulb generates significant amounts of ozone, which is toxic. A Lambda-DG-Series system
installed with a full spectrum bulb must be connected to a ventilation or ozone-removing
system (also known as an “ozone eater”) for the evacuation of the ozone produced during
operation. Please contact Sutter Instrument ([phone] or [e-mail])
for further information. There are no ventilation requirements for the ozone-free bulb.
Avoiding Physical Injury and Equipment Damage while Replacing the Xenon Bulb
WARNING: Before removing the xenon arc lamp bulb housing assembly
from a Lambda LS or Lambda DG-Series system, make certain that the system is powered
down (single switch for the Lambda LS or both MAIN and LAMP switches for the Lambda
DG-4-series) and disconnect the power cord from the source. Wait at least half an hour for
the lamp to cool before removing the lamp bulb housing.
The original manufacturer of the xenon lamp and power supply used in the Lambda DG-
Series provides the additional safety information on the following page. It is intended to
amplify the information given above.

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PerkinElmer Optoelectronics
44370 Christy Street
Fremont CA 94538
Phone: [phone]
FAX: [phone]
SHORT ARC XENON LAMPS AND
SYSTEMS
Proper use and safe operating practices are the
responsibility of equipment manufacturers who
incorporate the lamp into equipment and users of
such lamps and equipment. The supplier of this
lamp provides information on its products and
associated hazards, but it assumes no
responsibility for after-sale operating and safety
practices. All lamps are under pressure and must
be handled with care. Take appropriate action
through baffles, light shields, interlock switches or
other safeguards to protect personnel from harm
due to operation and/or failure of the lamp.
SAFE OPERATING INSTRUCTIONS
Do not operate this lamp except in accordance
with proper operating instructions and within
recommended operating specifications. Direct
questions regarding lamp operation or safety to
your lamp supplier.
LAMP DISPOSAL
CERMAX lamps do not have reclaimable parts.
Before disposal, it is recommended to relieve a
lamp’s gas pressure by squeezing the tip-off with
pliers until the gas escapes. If gas pressure is not
relieved, care should be taken to discard the lamp
in a landfill and not an incinerator.
SAFETY HAZARDS
The operation of lamps involves one or more of
the following hazards. In the absence of safe
operating practices and precautions, any one of
these hazards could result in injury.
I. EXPLOSION - The lamps are filled with
xenon gas at very high pressure. Lamps must be
handled with the same care and caution given
any vessel containing these levels of pressure. A
hazard exists if the window or ceramic fractures
and may cause explosive mechanical failure.
Face shields or proper safety glasses are
recommended during all handling operations.
II. HIGH VOLTAGE - Ignition voltage of some
lamp models is very high and can be deadly. If
portions of the circuit are exposed, caution must
be used in setup and operation of the system.
The input power must be disconnected from the
power source before attempting any service to
the lamp.
III. INFRARED AND ULTRAVIOLET
RADIATION - Do not look directly at operating
lamps orb reflected light. Infrared and ultra
violet radiation generated by the lamp can cause
skin burns and permanent eye damage.
IV. OZONE - Some UV type lamps generate
ozone, a toxic gas, by virtue of the ultraviolet
radiation. A lamp which gives off ozone must be
operated in a well ventilated area..
V. HOT SURFACES - Portions of the lamp can
reach temperatures of several hundred degrees
centigrade and cause serious burns if touched
even after the lamp is turned off.
OPERATING HAZARDS
CERMAX® Lamps
Read the following instructions and take all necessary
precautions

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TABLE OF CONTENTS
DISCLAIMER ......................................................................................................................................iii
SAFETY WARNINGS AND PRECAUTIONS...................................................................................iii
Electrical .................................................................................................................................................iii
Avoiding Electrical Shock and Fire-related Injury.............................................................................iii
Electromagnetic Interference ...............................................................................................................iv
Operational .............................................................................................................................................iv
Avoiding Physical Injury while Powered Up and Emitting Light ....................................................iv
Avoiding Physical Injury and Equipment Damage while Replacing the Xenon Bulb..................... v
1. GENERAL INFORMATION ...........................................................................................................1
1.1 Introduction.......................................................................................................................................1
1.1.1 How to Use this Manual............................................................................................................1
1.1.2 Technical Support ......................................................................................................................1
1.2 General Description..........................................................................................................................1
1.3 Differences between the Standard Lambda DG-4/5, the DG-4/5PLUS, and the DG-
4/5PLUS/USB..........................................................................................................................................2
1.4 Lambda DG-4PLUS/USB and Lambda DG-5PLUS/USB............................................................3
1.4.1 Systems........................................................................................................................................3
2. INSTALLATION ..............................................................................................................................5
2.1 Unpacking..........................................................................................................................................5
2.2 Getting Started..................................................................................................................................5
2.3 Light Guide & Optical Holder Installation ....................................................................................8
2.3.1 Liquid Light Guide.....................................................................................................................8
2.3.2 Liquid Light Guide Installation................................................................................................8
2.3.3 Optical Holder ............................................................................................................................9
2.4 Loading Filters ................................................................................................................................10
2.4.1 Filter Orientation.....................................................................................................................10
2.4.2 Loading Filters .........................................................................................................................10
2.4.3 Selection of a Filter Position...................................................................................................11
2.4.4 Blanking Discs ..........................................................................................................................11
2.4.5 Neutral Density Filters............................................................................................................11
2.4.6 Filter Holder Installation ........................................................................................................11
3. OPERATIONS ................................................................................................................................13
3.1 Control Electronics .........................................................................................................................13
3.2 Preparation and Precautions.........................................................................................................13
3.3 Power-Up Sequence........................................................................................................................13
3.4 Top Panel Controls .........................................................................................................................14
3.4.1 Keypad.......................................................................................................................................14
3.5 Electrical Connections....................................................................................................................15
3.5.1 Parallel Port..............................................................................................................................16
3.5.2 Serial Port .................................................................................................................................16
3.5.3 USB Port...................................................................................................................................16
3.5.4 Filter Value Out .......................................................................................................................17
3.5.5 SHUTTER ................................................................................................................................17

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3.5.6 STROBE....................................................................................................................................17
3.5.7 DAC............................................................................................................................................17
3.5.8 Line Power................................................................................................................................17
3.6 Fuse ..................................................................................................................................................17
3.7 Modes of Operation (LOCAL)........................................................................................................17
3.7.1 General Information ................................................................................................................17
3.7.2 Selecting an Operational Mode: The MODE Key.................................................................18
3.7.3 Move on Command Mode........................................................................................................19
3.7.4 Move on Sync Pulse Mode.......................................................................................................20
3.7.5 Move on Strobe Pulse Mode....................................................................................................21
3.7.6 Move on Sync, Inhibited by the Strobe Line Mode ..............................................................22
3.7.7 Edit a Filter Definition Mode..................................................................................................23
3.7.7.1 General Information .........................................................................................................23
3.7.7.2 Filter Numbers ..................................................................................................................24
3.7.7.3 Attenuation ........................................................................................................................25
3.7.7.4 Programming a Filter Number (“Logical Filter”)\........................................................26
3.7.7.5 Manual Adjustment of Attenuation ................................................................................27
3.7.8 Setup Ring Buffer Mode ..........................................................................................................28
3.7.8.1 General Information .........................................................................................................28
3.7.8.2 Storing the FILTER NUMBER Sequence .....................................................................29
3.7.8.3 Viewing an Existing FILTER NUMBER Sequence ......................................................30
3.7.8.4 Running the RING BUFFER Sequence .........................................................................31
3.7.9 Turbo-Blanking Mode..............................................................................................................32
3.7.10 Display the First Four Filter Values Mode .........................................................................33
4. EXTERNAL CONTROL OPERATIONS......................................................................................35
4.1 Parallel Interface.............................................................................................................................46
4.1.1 Connecting to the Lambda DG-4 parallel port .....................................................................46
4.1.2 Input Lines................................................................................................................................46
4.1.3 Command Codes.......................................................................................................................47
4.1.4 Busy Line ..................................................................................................................................47
4.1.5 Interface Modes ........................................................................................................................48
4.1.6 Controlling the Lambda DG-4 from a Computer’s Parallel (Printer) Port.......................48
4.1.7 Reading the Status of the Lambda DG-4...............................................................................50
4.1.8 Shutter Control ........................................................................................................................52
4.2 Serial Interface................................................................................................................................52
4.2.1 Connecting to the Lambda DG-Series Serial Input .............................................................52
4.2.2 Controlling the Lambda DG-4 from a Computer’s Serial Port...........................................53
4.2.3 Serial Port Command Echo ....................................................................................................54
4.2.4 Serial Port Command Completion Indicator ........................................................................54
4.2.5 Reading the Status of the Lambda DG-4...............................................................................54
5. MAINTENANCE............................................................................................................................59
5.1 Changing the Xenon Arc Bulb.......................................................................................................59
5.1.1 Bulb Assembly ..........................................................................................................................59
5.1.2 Removing the Lamp Assembly from the System..................................................................60
5.1.3 Bulb Replacement ....................................................................................................................61
5.1.3.1 Removing the Existing Bulb ............................................................................................61
5.1.3.2 Installing the New Bulb....................................................................................................61