Thermo Scientific 2122 User manual

056-720-00 Rev. 1
Cryogenics Thermoflasks
2122, 2123, 2124, 2129, and 2130
Important ead these operating instructions. Failure to read, understand
and follow the instructions in this manual may result in damage to the
unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by
qualified service personnel. s
©2017 Thermo Fisher Scientific. All rights reserved.
Application
Catalog Number
Operating Manual

It is recommended that care be taken in the use of Thermo Scientific
Thermoflasks. Any product used under vacuum is a potential hazard and,
therefore, should be treated with caution. If in doubt, consult the safety
regulations of your laboratory or institution. Specific warnings are listed
below to avoid some of the more common errors.
Failure to follow proper procedures, heed warnings, and cautions can result in
failure of the flask with potential expelling of the contents and subsequent harm
to user.
Warning Contact of liquid nitrogen with the skin or eyes may cause serious
(freezing) injury. When handling liquid gases, appropriate Personal
Protection Equipment (PPE) must be worn, such as goggles or facemasks,
and insulated or rubber gloves large enough to allow quick removal, and
insulated or rubber aprons. s
Caution A potentially dangerous situation could result from pouring liquid
gases into a Thermoflask without pre-cooling the flask. When pouring
liquefied gases from one container to another, the receiving container
should be cooled gradually to prevent thermal shock. The liquid should be
poured slowly to avoid splashing. A receiving vessel should always be
vented to the atmosphere and high concentrations of excess oxygen and/or
nitrogen should not be allowed to collect. s
Warning Nitrogen gas can cause suffocation without warning. Store and use
liquid nitrogen only in a well-ventilated place. As the liquid evaporates, the
resulting gas displaces the normal air in the area. (The cloudy vapor that
appears when liquid nitrogen is exposed to the air is condensed moisture,
not the gas itself. The issuing gas is invisible.) In closed areas, excessive
amounts of nitrogen gas reduces the concentration of oxygen and can result
in asphyxiation. Because nitrogen gas is colorless, odorless, and tasteless, it
cannot be detected by the human senses. Breathing an atmosphere that
contains less than 19.5% oxygen can cause dizziness and quickly result in
unconsciousness and death. Therefore, the use of oxygen monitoring
equipment is strongly recommended. s
Caution It is recommended to have this vessel tested by the manufacturer or
qualified cryovessel service technician every 7-10 years, regardless of any
problems (or lack thereof) there may have been in the past. This will help
insure your samples against sudden loss of liquid nitrogen due to vacuum
failure. s
Warning All Thermoflasks in stainless steel containers (catalog numbers
2122~2124) have vented lids to prevent build-up of gas pressure when
holding a gassing substance. The ability of the vent to release pressure
should be checked periodically. s
Handling Thermoflasks
2 Thermoflasks

The holding times in the table on the left are intended as a guide only, to
illustrate the probable time it takes for a stainless steel Thermoflask, with
lid, to empty completely after being filled with liquid nitrogen. The times
may change depending on higher or lower ambient temperatures, whether
or not the lid is removed for examination of the contents, and according
to the mass of the material being frozen.
Even though the Thermoflasks may be almost empty of liquid nitrogen,
they will continue to be effective storage vessels for frozen material because
the contents will be held in the vapor phase of the nitrogen, i.e. the liquid
nitrogen will have boiled to become a very low temperature gas. This can
benefit users who wish to store materials in a frozen state, but not at the
exceptionally low temperature of the liquid nitrogen itself.
Liquid Nitrogen Holding Times
3Thermoflasks
Handling Thermoflasks (cont.)
Size Holding Time
1 Liter 38 Hours
2 Liters 48 Hours
4.5 Liters 54 Hours
Specifications Catalog
Number
Capacity -
Liters
Inside
Diameter -
Inches (cm)
Outside
Diameter -
Inches (cm)
Height -
Inches (cm)
Ship Wt. -
lbs (kg)
2122 1.0 3.6 (9.1) 4.6 (11.7) 9.0 (22.9) 6 (2.7)
2123 2.0 4.2 (10.7) 5.6 (14.2) 10.7 (26.9) 8 (3.6)
2124 4.5 5.9 (15.0) 7.2 (18.2) 13.8 (35.0) 10 (4.5)
2129 1000ml 5.1 (13.0) 6.3 (16.0) 4.5 (11.6) 8 (3.6)
2130 1900ml 6.1 (15.5) 7.3 (18.5) 5.4 (13.7) 10 (4.5)
Warning Do not cover any glass Thermoflask with any fixed or heavy
object that might form a seal around the rim either naturally, or induced
by freezing. s
Warning If liquid gases or other materials of extremely low temperatures are
being used, ONLY use a stirring rod with a PTFE
(polytetrafluoroethylene) or comparable form of protective coating, to mix
or stir substances in the Thermoflask. s

4 Thermoflasks
Before using your any vessel, carefully inspect it. Check for signs of
damage, such as scratches, chips or discoloration of glass. If any damage is
found, do not use! It is advisable to fill (see Filling Instructions) all new
units with liquid nitrogen and watch liquid nitrogen loss rate for a few
days. If there are any problems, call Technical Services as soon as possible.
Use a wooden yardstick to measure liquid nitrogen level. Level will be
indicated by frost line which develops when dipstick is removed and waved
in a back and forth motion away from the user.
To avoid damage to your cryogenic storage vessel which may result in
premature vacuum loss, it is important that the following procedure be
used during the addition of liquid nitrogen to a warm vessel:
1. Add only a small amount of liquid nitrogen (10% of unit volume) to
new or warm vessels.
2. Allow this small amount of liquid nitrogen to sit in the covered vessel
for a minimum of 2 hours. This will limit stress caused by the sudden
temperature change associated with adding liquid nitrogen to a warm
vessel.
3. Add an additional 20% of unit volume of LN2to vessel.
4. Allow vessel to sit for 48 hours and monitor liquid nitrogen
consumption.
5. Fill vessel as desired. emember to allow for displacement of liquid
nitrogen when canisters and canes are inserted.
6. Insert and remove samples slowly. Allow liquid nitrogen to run out of
samples.
Caution Never overfill liquid nitrogen vessels. s
Operation
Glass Lined Dewar
Sealed Vacuum
Container

5Thermoflasks
THERMO FISHER SCIENTIFIC STANDARD PRODUCT WARRANTY
(LN2Vacuum)
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping
time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protec-
tion extends to any subsequent owner during the first year warranty period.
During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced
at Thermo's expense, labor included. LN2Vacuum Integrity is covered for one year. Installation and calibration are not cov-
ered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and
direction prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give prior
approval for return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to
Thermo postage paid and replacement parts are shipped FOB destination.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost
profits or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or
1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and
special application. Outside the USA, contact your local distributor for warranty information.
Rev. 0 5/10
ISO
9001
REGISTERED
Instruction Sheet 056-720-00 (7002122)
1 41172 9/21/16 pdated information and safety symbols ccs
0 -- 1/15/10 Original (was 056-720-00 6/10/08) ccs
Rev ECR/ECN Date Description By
Thermo Fisher 401 Millcreek Road [phone]
Scientific Marietta, OH 45750 [phone]
Material in these instructions are for information purposes only. The contents
and the product it describes are subject to change without notice. Thermo
Fisher Scientific makes no representations or warranties with respect to these
instructions. In no event shall Thermo be held liable for any damages, direct
or incidental, arising out of or related to the use of this manual.