Thermo Scientific Thermo Scientific Microm HM550 Series User manual

1Thermo Fisher Scientific Operation Manual HM550
Part of Thermo Fisher Scientific

02 Thermo Fisher Scientific
Operation Manual HM550

03Thermo Fisher Scientific Operation Manual HM550
Microm HM550
Thermo Scientific
CRYOSTAT MICROTOM
incl. options O M V P D
Operation manual – English
ex Ser. No 46320

04 Thermo Fisher Scientific
Operation Manual HM550
© 2010 ermo Fisher Scientific. All rights reserved.
ermo Fisher Scientific Microm International GmbH is an ISO 9001 Company.
ermo Scientific is the trading name of ermo Fisher Scientific Microm International GmbH.
All other trademarks are the property of ermo Fisher Scientific and its subsidiaries. ermo Fisher
Scientific makes every attempt to ensure that the information contained in this support documentation is
correct and clearly stated but does not accept responsibility for any errors or omissions. e development of
ermo Scientific products and services is an ongoing process. Please ensure that any published information
you use as a reference is up to date and relates to the condition of the product. If necessary, check with ermo
Fisher Scientific or your local ermo Fisher Scientific representative.
is Operation Manual may not, in whole or in part, be copied, photocopied, reproduced, translated or con-
verted to any electronic or other form without prior written consent of ermo Fisher Scientific.
All information contained in this manual is proprietary and confidential, and the exclusive property of ermo
Fisher Scientific. is manual is protected by copyright and any reproduction is prohibited. It is only be used
by the individuals to whom it has been made available through ermo Fisher Scientific.
Contact addresses Anatomical Pathology
Microm International GmbH
part of ermo Fisher Scientific
Otto-Hahn-Str. 1 A
69190 Walldorf
Germany
Tel: [phone]
Fax: [phone]
www.thermoscientific.com
info.dxd.dewal@thermofisher.com
ermo Fisher Scientific
4481 Campus Drive
Kalamazoo
MI 49008, USA
Tel: [phone]
Fax: [phone]
www.thermo.com/pathology
e ermo Scientific Microm HM550 meets the following CE Mark requirements:
In Vitro Diagnostic Directive 98/79/EC
Sanosil® is a protected trademark of Sanosil AG, Switzerland
02/11

05Thermo Fisher Scientific Operation Manual HM550
Figure 1. Cryostat Thermo Scientific Microm HM550.

06 Thermo Fisher Scientific
Operation Manual HM550
ermo Fisher Scientific Microm International GmbH certifies that this
instrument has been tested and checked carefully. Its technical data was
verified before shipment to be in accordance with the published specifi-
cations.
e instrument complies with applicable international safety regula-
tions.
is ermo Fisher Scientific product is warranted against defects in
material and workmanship for a period of 1 year. Parts which prove to
be defective during the warranty period will be repaired or replaced free
of charge by the manufacturer. No other warranty is expressed or im-
plied. Unauthorized modification or repair by third party persons will
void the warranty.
e warranty will expire in case of improper or wrong use of the instru-
ment and in case the warning and precautionary messages are not ob-
served. ermo Fisher Scientific is not liable for any occurring damage.
Errors and omissions excepted. Subject to amendment and improve-
ment without further notice.
is operation manual will be supplied together with each instrument.
Further copies can be ordered at the nearest ermo Fisher Scienitific
sales office by giving the serial number of the instrument, the number of
the operation manual and the date of issue.
is operation manual is available in the following languages:
German, English, French, Spanish, Italian
Cat. No: 388092
Certification
Warranty
Preface

07Thermo Fisher Scientific Operation Manual HM550
Dear valued Customer,
ank you for buying this ermo Fisher Scientific instrument.
Before putting the instrument into operation, please read these operat-
ing instructions carefully to familiarize you with its proper operation
and functions.
e Cryostat series Microm HM550 are highly efficient cryostats for
sectioning techniques in routine and research.
Only skilled or specially trained personnel must operate the microtome
cryostat, i.e. placing the specimen onto a chuck, sectioning and trans-
ferring sections onto a slide. e listed and marked safety measures as
well as the regulations and hygiene measures of your respective lab must
strictly be observed.
Serial No.:..................................................
Please check the Serial No. on the type plate, which is placed on the rear
side of your instrument and enter this number here. is way, questions
and service can be handled faster.
Issued on February 01st, 2011
Microm International GmbH
part of ermo Fisher Scientific
Otto-Hahn-Str. 1 A
69190 Walldorf
Germany
Tel: [phone]
Fax: [phone]
Email: info.dxd.dewal@thermofisher.com
Internet: www.thermoscientific.com
Intended use
Preface

08 Thermo Fisher Scientific
Operation Manual HM550
Table of Contents
Safety Precautions 11
Warning signals and symbols 11
Introduction 14
Description of the HM550 Series 14
Technical data HM550 Series 16
Operating instructions 24
Setting up the cryostat 24
Initial commissioning 29
Basic operational rules 30
Cutting process 34
Cutting movement and retraction 34
Setting section thickness and trimming thickness 35
Chamber cooling 36
Actual and set value of the chamber temperature 36
Function Fast freezing for standard instruments 37
Heat extractors (optional accessories) 38
Feed 40
Specimen coarse feed 40
Speed of the specimen coarse feed 41
Setting the cutting window 42
Automatic approach system 43
Trimming and first cuts 46
Fine feed 46
Turning on/off the function retraction 47
Indication of cutting processes 48
Section counter 49
Sum of section thickness 50
Remaining travel to front end position 50
Setting the real time, WAKE time, ACTIVE time and date 51
Setting the real time 51
Setting the WAKE time 53
Setting the ACTIVE time 54
Setting the date 56
Defrosting 58
Setting the defrosting time 58
Defrosting cycle 60
Interrupting a defrosting cycle 62
Emptying the defrosting liquid 63
Customer-specific settings 64
Setting the contrast 64
Selecting the language 65
Illumination of the cooling chamber 67
Service settings 68
Chapter 1
Chapter 3
Chapter 2
Table of Contents

09Thermo Fisher Scientific Operation Manual HM550
Chapter 4
Specimen orientation 69
Knife carriers 70
Standard knife carrier 70
Disposable blade carrier EC 72
Disposable blade carrier EC 70 74
Magnetic disposable blade carrier MC 76
Option O – specimen cooling 77
Actual and set value of the specimen temperature 77
Specimen orientation with zero device (with option O) 79
Option M – motorized cutting drive 80
Setting the cutting window 80
Selecting the operating modes 81
Setting the cutting speed 83
Start and stop of the cutting drive 83
Hand wheel brake 84
Emergency stop 84
Option V – Vacutome 87
Setting the vacuum for stretching sections and disposing of
section waste 87
Inserting and replacing the filter unit 88
Setting the suction window 94
Knife carrier for Vacutome 95
Disposable blade carrier EV 95
Magnetic disposable blade carrier MV 98
Option P – active deep freezing device with Peltier element 100
Cold D (Option DC) function 103
Preparing to perform Cold D 105
Starting Cold D 107
Interrupting Cold D 107
Programming Cold D 108
orough cleaning of the cooling chamber 113
Error code indication 114
Definition of the error codes 114
Error codes of the Cold D (Option DC) function 115
Accessories 117
Standard equipment 117
Additional equipment 118
Theory of operation 119
Specimen and chamber cooling, defrosting 119fig
Cutting movement 121
Specimen coarse feed and trimming function 122
Automatic approach system 122
Option M – Motorized cutting drive 123
Option V – Stretching sections and disposing of section waste 123
Option O – Specimen fast and temperature control 124
Cold D (Option DC) 125
Table of Contents

10 Thermo Fisher Scientific
Operation Manual HM550
Working with the cryostat 125
Preparations on the microtome and inside the cooling chamber 125
Freezing-on the specimen 126
Orientation and trimming of specimens 126
Sectioning and taking off sections 127
How to avoid malfunctions 128
Possible sources of errors – cause and removal 130
Maintenance and care of the cryostat 132
Shutting-off for cleaning 132
Removing the microtome 133
Cleaning and care of the microtome 136
Cleaning the cooling lamella 137
Changing the fluorescent lamp 139
Conditions for the transportation of the instrument 140
Taking back the instrument for repair or routine maintenance 140
Disposal of the instrument after final shutdown 143
Cryostat disinfection 144
Recommended Disinfection Procedure 144
Certificate 145
Chapter 5
Chapter 6
Chapter 7
Appendix A
Table of Contents
Appendix B

11Thermo Fisher Scientific Operation Manual HM550
Safety Precautions
Warning singals and symbols
Warning signals
and symbols
Chapter 1
Safety precautions
e installation and routine use of the HM550 Series is easy and safe if
the instructions in this manual are being observed.
Note:
Special instructions regarding operation of the instrument.
WAꢀꢁIꢁꢂ
Sꢀꢁꢂꢃꢄꢅ ꢀꢆꢁꢂꢄꢇꢈꢃꢉꢊꢄꢆꢋ ꢌꢁꢄꢍꢇꢆꢁꢍ ꢈꢉ ꢀꢆꢁꢎꢁꢊꢈ ꢏꢄꢌꢄꢐꢁ ꢈꢉ ꢁꢑꢇꢃꢀ-
ꢌꢁꢊꢈ. Fꢉꢆ ꢄ ꢅꢉꢊꢐ ꢅꢃꢒꢁꢈꢃꢌꢁ ꢉꢒ ꢈꢓꢁ ꢁꢑꢇꢃꢀꢌꢁꢊꢈ, ꢀꢅꢁꢄꢍꢁ ꢉꢔꢍꢁꢆꢎꢁ ꢈꢓꢁꢍꢁ
ꢃꢊꢍꢈꢆꢇꢂꢈꢃꢉꢊꢍ ꢂꢄꢆꢁꢒꢇꢅꢅꢋ.
CAꢃTIOꢁ – general danger spot
Tꢓꢁ ꢉꢀꢁꢆꢄꢈꢃꢉꢊ ꢌꢄꢊꢇꢄꢅ ꢌꢇꢍꢈ ꢍꢈꢆꢃꢂꢈꢅꢋ ꢔꢁ ꢉꢔꢍꢁꢆꢎꢁꢏ ꢕꢓꢁꢊꢁꢎꢁꢆ ꢈꢓꢃꢍ
ꢍꢋꢌꢔꢉꢅ ꢃꢍ ꢎꢃꢍꢃꢔꢅꢁ ꢉꢊ ꢈꢓꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ.
Biohazard
Wꢄꢆꢊꢃꢊꢐ ꢉꢒ ꢔꢃꢉꢅꢉꢐꢃꢂꢄꢅ ꢏꢄꢊꢐꢁꢆ.
radioactivity
Wꢄꢆꢊꢃꢊꢐ ꢉꢒ ꢆꢄꢏꢃꢉꢄꢂꢈꢃꢎꢁ ꢏꢄꢊꢐꢁꢆ.
chemicals
Wꢄꢆꢊꢃꢊꢐ ꢉꢒ ꢇꢊꢓꢁꢄꢅꢈꢓꢋ ꢉꢆ ꢃꢆꢆꢃꢈꢄꢈꢃꢊꢐ ꢍꢇꢔꢍꢈꢄꢊꢂꢁꢍ.
separate taking Back of electrical and electronic instruments in the coun-
tries of the european union:
Tꢓꢃꢍ ꢃꢍ ꢈꢉ ꢔꢁ ꢄꢀꢀꢅꢃꢁꢏ ꢃꢊ ꢈꢓꢁ ꢂꢉꢇꢊꢈꢆꢃꢁꢍ ꢉꢒ ꢈꢓꢁ Eꢇꢆꢉꢀꢁꢄꢊ Uꢊꢃꢉꢊ
ꢄꢊꢏ ꢉꢈꢓꢁꢆ Eꢇꢆꢉꢀꢁꢄꢊ ꢂꢉꢇꢊꢈꢆꢃꢁꢍ ꢕꢃꢈꢓ ꢄ ꢍꢁꢀꢄꢆꢄꢈꢁ ꢂꢉꢅꢅꢁꢂꢈꢃꢊꢐ ꢍꢋꢍ-
ꢈꢁꢌ ꢕꢃꢈꢓꢃꢊ ꢈꢓꢁ ꢕꢄꢍꢈꢁ ꢌꢄꢊꢄꢐꢁꢌꢁꢊꢈ. Tꢓꢃꢍ ꢀꢆꢉꢏꢇꢂꢈ, ꢔꢁꢃꢊꢐ ꢄꢊ ꢁꢅꢁꢂ-
ꢈꢆꢉ ꢄꢊꢏ/ꢉꢆ ꢁꢅꢁꢂꢈꢆꢉꢊꢃꢂ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ, ꢌꢇꢍꢈ ꢔꢁ ꢈꢆꢁꢄꢈꢁꢏ ꢍꢁꢀꢄꢆꢄꢈꢁꢅꢋ
ꢕꢃꢈꢓꢃꢊ ꢈꢓꢁ ꢕꢄꢍꢈꢁ ꢌꢄꢊꢄꢐꢁꢌꢁꢊꢈ ꢀꢆꢉꢂꢁꢍꢍ (WEEE).
CAꢃTIOꢁ!
Tꢓꢁ ꢉꢀꢁꢆꢄꢈꢉꢆ’ꢍ ꢍꢄꢒꢁꢈꢋ ꢃꢍ ꢄꢒꢒꢁꢂꢈꢁꢏ, ꢕꢓꢁꢊ ꢈꢓꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ ꢃꢍ ꢊꢉꢈ ꢉꢀ-
ꢁꢆꢄꢈꢁꢏ ꢃꢊ ꢄꢂꢂꢉꢆꢏꢄꢊꢂꢁ ꢕꢃꢈꢓ ꢈꢓꢃꢍ ꢉꢀꢁꢆꢄꢈꢃꢉꢊ ꢌꢄꢊꢇꢄꢅ. Pꢅꢁꢄꢍꢁ ꢉꢔꢍꢁꢆꢎꢁ
ꢈꢓꢁ ꢒꢉꢅꢅꢉꢕꢃꢊꢐ ꢐꢁꢊꢁꢆꢄꢅ ꢀꢆꢁꢂꢄꢇꢈꢃꢉꢊꢍ ꢏꢇꢆꢃꢊꢐ ꢉꢀꢁꢆꢄꢈꢃꢉꢊ ꢉꢒ ꢈꢓꢃꢍ
ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ. Fꢄꢃꢅꢇꢆꢁ ꢈꢉ ꢂꢉꢌꢀꢅꢋ ꢕꢃꢈꢓ ꢈꢓꢁꢍꢁ ꢀꢆꢁꢂꢄꢇꢈꢃꢉꢊꢍ ꢎꢃꢉꢅꢄꢈꢁꢍ
ꢍꢄꢒꢁꢈꢋ ꢍꢈꢄꢊꢏꢄꢆꢏꢍ ꢄꢊꢏ ꢈꢓꢁ ꢃꢊꢈꢁꢊꢏꢁꢏ ꢇꢍꢁ ꢉꢒ ꢈꢓꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ. Tꢓꢁꢆꢌꢉ
Fꢃꢍꢓꢁꢆ Sꢂꢃꢁꢊꢈꢃꢒꢃꢂ ꢃꢍ ꢊꢉꢈ ꢅꢃꢄꢔꢅꢁ ꢒꢉꢆ ꢌꢃꢍꢇꢍꢁ ꢉꢒ ꢈꢓꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈꢍ ꢄꢊꢏ
ꢒꢄꢃꢅꢇꢆꢁ ꢈꢉ ꢂꢉꢌꢀꢅꢋ ꢕꢃꢈꢓ ꢔꢄꢍꢃꢂ ꢍꢄꢒꢁꢈꢋ ꢆꢁꢑꢇꢃꢆꢁꢌꢁꢊꢈꢍ.

12 Thermo Fisher Scientific
Operation Manual HM550
instrument grounding
Tꢉ ꢄꢎꢉꢃꢏ ꢃꢊꢖꢇꢆꢋ ꢒꢆꢉꢌ ꢁꢅꢁꢂꢈꢆꢃꢂꢄꢅ ꢂꢇꢆꢆꢁꢊꢈ, ꢈꢓꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ ꢌꢇꢍꢈ
ꢔꢁ ꢂꢉꢊꢊꢁꢂꢈꢁꢏ ꢕꢃꢈꢓ ꢈꢓꢁ ꢀꢆꢉꢈꢁꢂꢈꢃꢎꢁ ꢁꢄꢆꢈꢓ. Tꢓꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ ꢃꢍ
ꢁꢑꢇꢃꢀꢀꢁꢏ ꢕꢃꢈꢓ ꢄ ꢈꢓꢆꢁꢁ ꢕꢃꢆꢁ ꢐꢆꢉꢇꢊꢏ ꢀꢅꢇꢐ. Tꢓꢁ ꢀꢉꢕꢁꢆ ꢉꢇꢈꢅꢁꢈ ꢌꢇꢍꢈ
ꢔꢁ ꢂꢉꢊꢊꢁꢂꢈꢁꢏ ꢈꢉ ꢈꢓꢁ ꢀꢆꢉꢈꢁꢂꢈꢃꢎꢁ ꢁꢄꢆꢈꢓ ꢄꢊꢏ ꢌꢇꢍꢈ ꢌꢁꢁꢈ ꢈꢓꢁ Iꢊꢈꢁꢆ-
ꢊꢄꢈꢃꢉꢊꢄꢅ Eꢅꢁꢂꢈꢆꢉꢈꢁꢂꢓꢊꢃꢂꢄꢅ Cꢉꢌꢌꢃꢍꢍꢃꢉꢊ (IEC) ꢆꢁꢐꢇꢅꢄꢈꢃꢉꢊꢍ.
caution: mains voltage
Nꢁꢎꢁꢆ ꢆꢁꢌꢉꢎꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ ꢂꢉꢎꢁꢆꢍ ꢏꢇꢆꢃꢊꢐ ꢉꢀꢁꢆꢄꢈꢃꢉꢊ. Cꢉꢌꢀꢉꢊꢁꢊꢈ
ꢆꢁꢀꢅꢄꢂꢁꢌꢁꢊꢈꢍ ꢄꢍ ꢕꢁꢅꢅ ꢄꢍ ꢄꢏꢖꢇꢍꢈꢌꢁꢊꢈꢍ ꢌꢇꢍꢈ ꢉꢊꢅꢋ ꢔꢁ ꢌꢄꢏꢁ ꢔꢋ
ꢈꢆꢄꢃꢊꢁꢏ ꢍꢁꢆꢎꢃꢂꢁ ꢀꢁꢆꢍꢉꢊꢊꢁꢅ. Uꢊꢀꢅꢇꢐ ꢈꢓꢁ ꢇꢊꢃꢈ ꢔꢁꢒꢉꢆꢁ ꢆꢁꢌꢉꢎꢃꢊꢐ ꢉꢆ
ꢉꢀꢁꢊꢃꢊꢐ ꢈꢓꢁ ꢂꢉꢎꢁꢆꢍ.
danger in explosive environment
Tꢓꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ ꢌꢇꢍꢈ ꢊꢉꢈ ꢔꢁ ꢉꢀꢁꢆꢄꢈꢁꢏ ꢃꢊ ꢈꢓꢁ ꢀꢆꢁꢍꢁꢊꢂꢁ ꢉꢒ ꢒꢅꢄꢌ-
ꢌꢄꢔꢅꢁ ꢐꢄꢍꢁꢍ.
hazard of frostBite
Aꢎꢉꢃꢏ ꢀꢁꢆꢌꢄꢊꢁꢊꢈ ꢈꢉꢇꢂꢓꢃꢊꢐ ꢉꢒ ꢌꢁꢈꢄꢅ ꢀꢄꢆꢈꢍ ꢃꢊꢍꢃꢏꢁ ꢈꢓꢁ ꢂꢆꢋꢉꢍꢈꢄꢈ ꢌꢃ-
ꢂꢆꢉꢈꢉꢌꢁ ꢂꢓꢄꢌꢔꢁꢆ ꢄꢍ ꢒꢆꢉꢍꢈꢔꢃꢈꢁ ꢌꢄꢋ ꢉꢂꢂꢇꢆ ꢄꢈ ꢇꢊꢀꢆꢉꢈꢁꢂꢈꢁꢏ ꢓꢄꢊꢏꢍ
ꢄꢊꢏ ꢄꢆꢌꢍ.
hazard of radioactive radiation
Wꢓꢁꢊ ꢕꢉꢆꢗꢃꢊꢐ ꢕꢃꢈꢓ ꢆꢄꢏꢃꢉꢄꢂꢈꢃꢎꢁ ꢍꢀꢁꢂꢃꢌꢁꢊꢍ ꢉꢔꢍꢁꢆꢎꢁ ꢄꢅꢅ ꢄꢀꢀꢅꢃꢂꢄ-
ꢔꢅꢁ ꢆꢄꢏꢃꢄꢈꢃꢉꢊ ꢍꢄꢒꢁꢈꢋ ꢀꢆꢉꢂꢁꢏꢇꢆꢁꢍ. Wꢓꢁꢊ ꢕꢉꢆꢗꢃꢊꢐ ꢕꢃꢈꢓ ꢆꢄꢏꢃꢉꢄꢂ-
ꢈꢃꢎꢁ ꢂꢉꢊꢈꢄꢌꢃꢊꢄꢈꢁꢏ ꢌꢄꢈꢁꢆꢃꢄꢅ, ꢄꢀꢀꢆꢉꢀꢆꢃꢄꢈꢁ ꢍꢄꢒꢁꢈꢋ ꢄꢊꢏ ꢏꢃꢍꢃꢊꢒꢁꢂꢈꢃꢉꢊ
ꢌꢁꢄꢍꢇꢆꢁꢍ ꢌꢇꢍꢈ ꢔꢁ ꢂꢄꢆꢆꢃꢁꢏ ꢉꢇꢈ. Aꢂꢂꢉꢆꢏꢃꢊꢐ ꢈꢉ ꢈꢓꢁ ꢆꢇꢅꢁꢍ ꢄꢊꢏ ꢆꢁꢐꢇ-
ꢅꢄꢈꢃꢉꢊꢍ ꢂꢉꢊꢂꢁꢆꢊꢃꢊꢐ ꢈꢓꢁ ꢓꢄꢊꢏꢅꢃꢊꢐ ꢉꢒ ꢆꢄꢏꢃꢉꢄꢂꢈꢃꢎꢁ ꢂꢉꢊꢈꢄꢌꢃꢊꢄꢈꢁꢏ
ꢌꢄꢈꢁꢆꢃꢄꢅ ꢉꢒ ꢈꢓꢁ ꢆꢁꢍꢀꢁꢂꢈꢃꢎꢁ ꢅꢄꢔꢉꢆꢄꢈꢉꢆꢋ, ꢍꢄꢒꢁꢈꢋ ꢂꢅꢉꢈꢓꢃꢊꢐ (ꢁ.ꢐ.
ꢀꢄꢆꢈꢃꢂꢅꢁ ꢌꢄꢍꢗ, ꢐꢅꢉꢎꢁꢍ, ꢀꢆꢉꢈꢁꢂꢈꢃꢎꢁ ꢍꢓꢉꢁ ꢂꢉꢎꢁꢆꢍ) ꢌꢇꢍꢈ ꢔꢁ ꢕꢉꢆꢊ.
Rꢄꢏꢃꢉꢄꢂꢈꢃꢎꢁ ꢂꢉꢊꢈꢄꢌꢃꢊꢄꢈꢁꢏ ꢕꢄꢍꢈꢁ ꢌꢇꢍꢈ ꢔꢁ ꢏꢃꢍꢀꢉꢍꢁꢏ ꢉꢒ ꢄꢂꢂꢉꢆꢏꢃꢊꢐ
ꢈꢉ ꢈꢓꢁ ꢆꢁꢍꢀꢁꢂꢈꢃꢎꢁ ꢆꢁꢐꢇꢅꢄꢈꢃꢉꢊꢍ.
hazard of infection
Uꢍꢁ ꢈꢓꢁ ꢄꢀꢀꢆꢉꢀꢆꢃꢄꢈꢁ ꢍꢄꢒꢁꢈꢋ ꢄꢊꢏ ꢏꢃꢍꢃꢊꢒꢁꢂꢈꢃꢉꢊ ꢌꢁꢄꢍꢇꢆꢁꢍ ꢕꢓꢁꢊ
ꢕꢉꢆꢗꢃꢊꢐ ꢕꢃꢈꢓ ꢃꢊꢒꢁꢂꢈꢃꢉꢇꢍ ꢍꢀꢁꢂꢃꢌꢁꢊꢍ. Aꢂꢂꢉꢆꢏꢃꢊꢐ ꢈꢉ ꢈꢓꢁ ꢆꢇꢅꢁꢍ ꢄꢊꢏ
ꢆꢁꢐꢇꢅꢄꢈꢃꢉꢊꢍ ꢂꢉꢊꢂꢁꢆꢊꢃꢊꢐ ꢈꢓꢁ ꢓꢄꢊꢏꢅꢃꢊꢐ ꢉꢒ ꢃꢊꢒꢁꢂꢈꢃꢉꢇꢍ/ꢆꢄꢏꢃꢉꢄꢂ-
ꢈꢃꢎꢁ ꢂꢉꢊꢈꢄꢌꢃꢊꢄꢈꢁꢏ ꢌꢄꢈꢁꢆꢃꢄꢅ ꢉꢒ ꢈꢓꢁ ꢆꢁꢍꢀꢁꢂꢈꢃꢎꢁ ꢅꢄꢔꢉꢆꢄꢈꢉꢆꢋ, ꢍꢄꢒꢁꢈꢋ
ꢂꢅꢉꢈꢓꢃꢊꢐ (ꢁ.ꢐ. ꢀꢄꢆꢈꢃꢂꢅꢁ ꢌꢄꢍꢗ, ꢐꢅꢉꢎꢁꢍ, ꢀꢆꢉꢈꢁꢂꢈꢃꢎꢁ ꢍꢓꢉꢁ ꢂꢉꢎꢁꢆꢍ)
ꢌꢇꢍꢈ ꢔꢁ ꢕꢉꢆꢊ.
Biohazard
Sꢀꢁꢂꢃꢌꢁꢊꢍ ꢇꢍꢁꢏ ꢏꢇꢆꢃꢊꢐ ꢈꢓꢁ ꢃꢊꢈꢁꢊꢏꢁꢏ ꢉꢀꢁꢆꢄꢈꢃꢉꢊ ꢉꢒ ꢈꢓꢁ ꢃꢊꢍꢈꢆꢇ-
ꢌꢁꢊꢈ ꢌꢃꢐꢓꢈ ꢀꢉꢈꢁꢊꢈꢃꢄꢅꢅꢋ ꢔꢁ ꢃꢊꢒꢁꢂꢈꢃꢉꢇꢍ. Fꢉꢆ ꢈꢓꢃꢍ ꢆꢁꢄꢍꢉꢊ, ꢃꢈ ꢃꢍ
ꢆꢁꢂꢉꢌꢌꢁꢊꢏꢁꢏ ꢈꢉ ꢉꢔꢍꢁꢆꢎꢁ ꢈꢓꢁ ꢐꢁꢊꢁꢆꢄꢅ ꢅꢄꢔꢉꢆꢄꢈꢉꢆꢋ ꢆꢁꢐꢇꢅꢄꢈꢃꢉꢊꢍ
ꢂꢉꢊꢂꢁꢆꢊꢃꢊꢐ ꢀꢆꢉꢈꢁꢂꢈꢃꢉꢊ ꢄꢐꢄꢃꢊꢍꢈ ꢏꢄꢊꢐꢁꢆ ꢉꢒ ꢃꢊꢒꢁꢂꢈꢃꢉꢊ. Iꢊꢒꢉꢆꢌꢄꢈꢃꢉꢊ
ꢉꢊ ꢏꢁꢂꢉꢊꢈꢄꢌꢃꢊꢄꢈꢃꢉꢊ ꢌꢁꢏꢃꢄ, ꢈꢓꢁꢃꢆ ꢇꢍꢁ, ꢏꢃꢅꢇꢈꢃꢉꢊ ꢄꢊꢏ ꢁꢒꢒꢁꢂꢈꢃꢎꢁ
ꢆꢄꢊꢐꢁ ꢉꢒ ꢄꢀꢀꢅꢃꢂꢄꢈꢃꢉꢊ ꢂꢄꢊ ꢔꢁ ꢆꢁꢄꢏ ꢃꢊ ꢈꢓꢁ Lꢄꢔꢉꢆꢄꢈꢉꢆꢋ Bꢃꢉꢍꢄꢒꢁꢈꢋ
Mꢄꢊꢇꢄꢅ: ꢘꢙꢚꢛ ꢉꢒ ꢈꢓꢁ Wꢉꢆꢅꢏ Hꢁꢄꢅꢈꢓ Oꢆꢐꢄꢊꢃꢜꢄꢈꢃꢉꢊ.
Safety Precautions
Warning singals and symbols

13Thermo Fisher Scientific Operation Manual HM550
hazard of malfunction
Tꢉ ꢄꢎꢉꢃꢏ ꢈꢓꢁ ꢓꢄꢜꢄꢆꢏ ꢉꢒ ꢌꢄꢅꢒꢇꢊꢂꢈꢃꢉꢊ ꢉꢒ ꢄꢊ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ, ꢃꢈ ꢌꢇꢍꢈ
ꢉꢊꢅꢋ ꢔꢁ ꢉꢀꢁꢆꢄꢈꢁꢏ ꢃꢊ ꢄ ꢂꢉꢊꢈꢆꢉꢅꢅꢁꢏ ꢁꢅꢁꢂꢈꢆꢉꢌꢄꢐꢊꢁꢈꢃꢂ ꢁꢊꢎꢃꢆꢉꢊꢌꢁꢊꢈ.
Tꢓꢃꢍ ꢌꢁꢄꢊꢍ, ꢈꢓꢄꢈ ꢈꢆꢄꢊꢍꢌꢃꢈꢈꢁꢆꢍ ꢍꢇꢂꢓ ꢄꢍ ꢌꢉꢔꢃꢅꢁ ꢀꢓꢉꢊꢁꢍ ꢌꢇꢍꢈ ꢊꢉꢈ
ꢔꢁ ꢉꢀꢁꢆꢄꢈꢁꢏ ꢃꢊ ꢈꢓꢁꢃꢆ ꢂꢅꢉꢍꢁ ꢎꢃꢂꢃꢊꢃꢈꢋ.
Iꢊ ꢂꢄꢍꢁ ꢉꢒ ꢌꢄꢅꢒꢇꢊꢂꢈꢃꢉꢊꢍ ꢄꢊꢏ/ꢉꢆ ꢍꢁꢆꢎꢃꢂꢁ ꢕꢉꢆꢗ, ꢀꢅꢁꢄꢍꢁ ꢈꢇꢆꢊ ꢉꢒꢒ
ꢈꢓꢁ ꢃꢊꢍꢈꢆꢇꢌꢁꢊꢈ ꢄꢊꢏ ꢂꢉꢊꢈꢄꢂꢈ ꢋꢉꢇꢆ ꢅꢉꢂꢄꢅ ꢏꢁꢄꢅꢁꢆ.
Warning of unhealthy or irritating suBstances
Dꢇꢆꢃꢊꢐ ꢈꢓꢁ ꢃꢊꢈꢁꢊꢏꢁꢏ ꢇꢍꢁ ꢉꢒ ꢈꢓꢁ ꢉꢀꢈꢃꢉꢊ D, ꢍꢌꢄꢅꢅꢁꢍꢈ ꢄꢌꢉꢇꢊꢈꢍ
ꢉꢒ ꢃꢆꢆꢃꢈꢄꢈꢃꢊꢐ ꢄꢁꢆꢉꢍꢉꢅꢍ, ꢂꢉꢊꢍꢃꢍꢈꢃꢊꢐ ꢉꢒ ꢄꢂꢈꢃꢎꢄꢈꢁꢏ ꢉꢝꢋꢐꢁꢊ, ꢌꢃꢐꢓꢈ
ꢉꢂꢂꢇꢆ. Fꢉꢆ ꢈꢓꢃꢍ ꢆꢁꢄꢍꢉꢊ, ꢀꢅꢁꢄꢍꢁ ꢂꢄꢆꢁ ꢒꢉꢆ ꢍꢇꢒꢒꢃꢂꢃꢁꢊꢈ ꢎꢁꢊꢈꢃꢅꢄꢈꢃꢉꢊ.
Tꢉ ꢄꢎꢉꢃꢏ ꢊꢁꢐꢄꢈꢃꢎꢁ ꢁꢒꢒꢁꢂꢈꢍ ꢉꢊ ꢋꢉꢇꢆ ꢓꢁꢄꢅꢈꢓ ꢕꢓꢃꢅꢁ ꢃꢊ ꢏꢃꢆꢁꢂꢈ ꢂꢉꢊ-
ꢈꢄꢂꢈ ꢕꢃꢈꢓ ꢈꢓꢁ ꢏꢃꢍꢃꢊꢒꢁꢂꢈꢃꢉꢊ ꢌꢁꢏꢃꢇꢌ ꢉꢆ ꢃꢈꢍ ꢆꢁꢍꢃꢏꢇꢁꢍ, ꢄꢅꢕꢄꢋꢍ ꢕꢁꢄꢆ
ꢆꢁꢍꢀꢁꢂꢈꢃꢎꢁ ꢍꢄꢒꢁꢈꢋ ꢂꢅꢉꢈꢓꢃꢊꢐ (ꢁ.ꢐ. ꢀꢆꢉꢈꢁꢂꢈꢃꢎꢁ ꢐꢅꢄꢍꢍꢁꢍ, ꢅꢄꢔꢉꢆꢄꢈꢉꢆꢋ
ꢐꢅꢉꢎꢁꢍ).
care in using microtome knife
Tꢉ ꢏꢃꢌꢃꢊꢃꢍꢓ ꢈꢓꢁ ꢏꢄꢊꢐꢁꢆ ꢉꢒ ꢔꢁꢃꢊꢐ ꢃꢊꢖꢇꢆꢁꢏ ꢔꢋ ꢈꢓꢁ ꢗꢊꢃꢒꢁ ꢉꢆ ꢔꢅꢄꢏꢁ,
ꢇꢍꢁ ꢈꢓꢁ ꢗꢊꢃꢒꢁ ꢐꢇꢄꢆꢏ ꢕꢓꢁꢊ ꢄꢏꢖꢇꢍꢈꢃꢊꢐ ꢍꢀꢁꢂꢃꢌꢁꢊ ꢄꢊꢏ ꢗꢊꢃꢒꢁ. Iꢒ
ꢀꢉꢍꢍꢃꢔꢅꢁ, ꢈꢓꢁ ꢍꢀꢁꢂꢃꢌꢁꢊ ꢍꢓꢉꢇꢅꢏ ꢔꢁ ꢂꢅꢄꢌꢀꢁꢏ ꢃꢊ ꢔꢁꢒꢉꢆꢁ ꢈꢓꢁ ꢗꢊꢃꢒꢁ
ꢃꢍ ꢃꢊꢍꢁꢆꢈꢁꢏ ꢃꢊꢈꢉ ꢈꢓꢁ ꢗꢊꢃꢒꢁ ꢓꢉꢅꢏꢁꢆ. Bꢁꢒꢉꢆꢁ ꢂꢓꢄꢊꢐꢃꢊꢐ ꢈꢓꢁ ꢗꢊꢃꢒꢁ
ꢓꢉꢅꢏꢁꢆ, ꢄꢅꢕꢄꢋꢍ ꢆꢁꢌꢉꢎꢁ ꢔꢅꢄꢏꢁ ꢉꢆ ꢗꢊꢃꢒꢁ! Uꢊꢇꢍꢁꢏ ꢗꢊꢃꢎꢁꢍ ꢍꢓꢉꢇꢅꢏ ꢄꢅ-
ꢕꢄꢋꢍ ꢔꢁ ꢗꢁꢀꢈ ꢃꢊ ꢄ ꢗꢊꢃꢒꢁ ꢂꢄꢍꢁ. Nꢁꢎꢁꢆ ꢀꢅꢄꢂꢁ ꢈꢓꢁ ꢗꢊꢃꢒꢁ ꢕꢃꢈꢓ ꢈꢓꢁ ꢂꢇꢈ-
ꢈꢃꢊꢐ ꢁꢏꢐꢁ ꢇꢀꢕꢄꢆꢏꢍ. Nꢁꢎꢁꢆ ꢈꢆꢋ ꢈꢉ ꢂꢄꢈꢂꢓ ꢄ ꢏꢆꢉꢀꢀꢃꢊꢐ ꢗꢊꢃꢒꢁ!! Nꢁꢎꢁꢆ
ꢂꢓꢁꢂꢗ ꢈꢓꢁ ꢍꢓꢄꢆꢀꢊꢁꢍꢍ ꢉꢒ ꢈꢓꢁ ꢂꢇꢈꢈꢃꢊꢐ ꢁꢏꢐꢁ ꢕꢃꢈꢓ ꢋꢉꢇꢆ ꢒꢃꢊꢐꢁꢆꢍ. Tꢓꢁ
ꢂꢇꢈꢈꢃꢊꢐ ꢁꢏꢐꢁ ꢃꢍ ꢁꢝꢈꢆꢁꢌꢁꢅꢋ ꢍꢓꢄꢆꢀ!
Waste disposal
Aꢅꢅ ꢏꢁꢔꢆꢃꢍ, ꢕꢄꢍꢈꢁ, ꢏꢁꢒꢆꢉꢍꢈꢃꢊꢐ ꢅꢃꢑꢇꢃꢏ ꢄꢍ ꢕꢁꢅꢅ ꢄꢍ ꢃꢊꢒꢁꢂꢈꢃꢉꢇꢍ ꢄꢊꢏ
ꢆꢄꢏꢃꢉꢄꢂꢈꢃꢎꢁ ꢂꢉꢊꢈꢄꢌꢃꢊꢄꢈꢁꢏ ꢌꢄꢈꢁꢆꢃꢄꢅ ꢒꢆꢉꢌ ꢉꢀꢁꢆꢄꢈꢃꢉꢊ ꢌꢇꢍꢈ ꢔꢁ ꢏꢃꢍ-
ꢀꢉꢍꢁꢏ ꢉꢒ ꢃꢊ ꢄꢂꢂꢉꢆꢏꢄꢊꢂꢁ ꢕꢃꢈꢓ ꢈꢓꢁ ꢆꢁꢍꢀꢁꢂꢈꢃꢎꢁ ꢆꢁꢐꢇꢅꢄꢈꢃꢉꢊꢍ ꢉꢒ ꢈꢓꢁ
ꢅꢄꢔ. Dꢃꢍꢃꢊꢒꢁꢂꢈꢃꢉꢊ ꢄꢊꢏ ꢂꢅꢁꢄꢊꢃꢊꢐ ꢅꢃꢑꢇꢃꢏꢍ ꢄꢍ ꢕꢁꢅꢅ ꢄꢍ ꢍꢁꢂꢈꢃꢉꢊ ꢕꢄꢍꢈꢁ
ꢌꢇꢍꢈ ꢔꢁ ꢏꢃꢍꢀꢉꢍꢁꢏ ꢉꢒ ꢄꢂꢂꢉꢆꢏꢃꢊꢐ ꢈꢉ ꢈꢓꢁ ꢆꢁꢍꢀꢁꢂꢈꢃꢎꢁ ꢆꢁꢐꢇꢅꢄꢈꢃꢉꢊꢍ ꢒꢉꢆ
ꢍꢀꢁꢂꢃꢄꢅ ꢕꢄꢍꢈꢁ!
Safety Precautions
Warning singals and symbols

14 Thermo Fisher Scientific
Operation Manual HM550
Chapter 2
Introduction
Open top cryostat. Modular design. Stainless steel cooling chamber.
Chamber temperature regulation variable from -10°C down to -35°C.
When the instrument is equipped with the option O, the specimen
temperature can be controlled from +10°C down to -50°C.
Graphic LC-display of set and actual temperatures, electronic control
with user-oriented touchpad keyboard, battery-buffered memory and
self-diagnostic system. Standby and sleep status for noise reduction and
energy savings. Automatic defrosting and in addition a manual defrost
cycle which can be activated when needed. Defrost interrupt on key-
stroke request. Integrated fast freezing device with controlled cooling
element down to -60°C (option P). Sliding window with heater and
integrated fluorescent lamp for cryo-chamber. Brush shelf and storage
space inside the cryo-chamber. Large, flat storage space on top of the
housing with integrated storage space for marked slides.
Rotary Microtome with backlash and maintenance-free cross roller
bearings in stainless steel. Electro-mechanical feed system.
Section thickness setting from 1 to 100 microns;
up to 10 µm in 1 µm-increments,
up to 20 µm in 2 µm-increments,
up to 70 µm in 5 µm-increments and
up to 100 µm in 10 µm-increments.
Trimming thickness setting from 5 to 500 microns;
up to 10 µm in 5 µm-increments,
up to 100 µm in 10 µm-increments,
up to 200 µm in 20 µm-increments and
up to 500 µm in 50 µm-increments.
Specimen retraction during the return stroke with optical indication.
Horizontal feed range 28 mm. Vertical cutting stroke 60 mm. Max.
specimen size 75 x 55 mm.
Motorized coarse feed in two directions with three speed selections.
Limit indication and automated switch off at front and rear limits of
horizontal travel. Automatic approach system for exact and safe ap-
proach of specimen towards the knife edge from -5°C to -35°C.
Section counter and indication of sum of section thicknesses with reset-
button. Indication of remaining travel.
Hand wheel brake in any position. Fine orientation of specimen with
zero device. Rotatable on Z-axis, 360°.
Description of the
HM550 Series
Introduction
Description of the HM550 Series

15Thermo Fisher Scientific Operation Manual HM550
Basic outfit with three specimen chucks, 118 ml freezing medium, 100 ml
cryostat oil, brush shelf and section waste tray, consisting of two parts.
Electric motor drive with electronic control of the cutting stroke.
Cutting speed variable from 0 to 250 mm/s. Accelerated return travel.
Freely selectable cutting zone.
ree modes of operation: interval, single and continuous stroke. Indi-
cation of hand wheel brake.
Manual control of motor drive via safety start procedure. Stop com-
mand selection via manual control, foot pedal or hand wheel brake.
Emergency stop key and foot pedal emergency stop.
Vacutome system for stretching cryo-sections as well as for the disposal
of sections when trimming.
e system consists of a duo filter system: e coarse filter collects the
section waste. e micro filter with a 99.99% filtration efficiency for
0.1 µm particles filters the air. e coarse filter is actively cooled in its
position in the cryostat.
A vacuum-generating unit with its controlling elements is also part of
the system.
Stretching and disposing of is controlled by the cutting window that
can be set on the microtome cryostat. e suction vacuum can be set
via an turning knob on the microtome cryostat.
e Cold D (Option DC) is a fumigation unit for the application of
disinfection media (Sanosil®) onto the operation areas in the cryo cham-
ber which are accessible for the user during the intended use. Please
refer to the Recommended Disinfection Procedure in Appendix A at
the end of this manual.
Warning
Tꢉ ꢐꢇꢄꢆꢄꢊꢈꢁꢁ ꢈꢓꢁ ꢒꢇꢊꢂꢈꢃꢉꢊꢄꢅꢃꢈꢋ ꢄꢊꢏ ꢆꢁꢅꢃꢄꢔꢃꢅꢃꢈꢋ ꢉꢒ ꢈꢓꢁ ꢒꢇꢌꢃꢐꢄ-
ꢈꢃꢉꢊ ꢇꢊꢃꢈ, ꢃꢈ ꢃꢍ ꢆꢁꢂꢉꢌꢌꢁꢊꢏꢁꢏ ꢈꢉ ꢍꢉꢅꢁꢅꢋ ꢇꢍꢁ Sꢄꢊꢉꢍꢃꢅ®. Wꢓꢁꢊ ꢇꢍꢃꢊꢐ
ꢉꢈꢓꢁꢆ ꢏꢃꢍꢃꢊꢒꢁꢂꢈꢃꢉꢊ ꢌꢁꢏꢃꢄ, Tꢓꢁꢆꢌꢉ Sꢂꢃꢁꢊꢈꢃꢂ ꢄꢍꢍꢇꢌꢁꢍ ꢊꢉ ꢅꢃꢄꢔꢃꢅꢃꢈꢋ
ꢂꢉꢊꢂꢁꢆꢊꢃꢊꢐ ꢒꢇꢊꢂꢈꢃꢉꢊꢄꢅꢃꢈꢋ ꢄꢊꢏ ꢏꢃꢍꢃꢊꢒꢁꢂꢈꢃꢉꢊ ꢁꢒꢒꢁꢂꢈꢃꢎꢁꢊꢁꢍꢍ.
Option M
Option V
Cold D (Option DC)
Introduction
Description of the HM550 Series

16 Thermo Fisher Scientific
Operation Manual HM550
HM550 Series
Specimen temperature
control +10°C to -50°C
Chamber temperature
control -10°C to -35°C
Fast freezing station
depending on the chamber
temperature
up to -35°C
Actively cooled fast free-
zing station with Peltier
element
max. up to -60°C
Defrosting automatic defrosting,
adjustable with temperature control
manual immediate defros-
ting
Microtome Section thickness range 1 to 500 µm
Fine section thickness range 1 to 100 µm
Resolution 1 µm from 1 to 10 µm
2 µm from 10 to 20 µm
5 µm from 20 to 70 µm
10 µm from 70 to 100 µm
Trimming thickness range 5 to 500 µm
Resolution 5 µm from 5 to 10 µm
10 µm from 10 to 100 µm
20 µm from 100 to 200 µm
50 µm from 200 to 500 µm
Specimen retraction during
return travel 40 µm
Vertical specimen stroke 60 mm
Horizontal specimen
movement 28 mm
Read-outs Graphical LC display:
section thicknesses, section
counter, sum of section
thicknesses, remaining
travel to front end position,
size of the cutting window,
chamber temperature,
specimen temperature
and graphic of temperatu-
res, menu with submenus
Specimen approach automatic function
alternatively manual with
variable speed
Size of chucks 30 and 40 mm
special sizes upon request
Technical Data
Introduction
Technical data HM550 Series

17Thermo Fisher Scientific Operation Manual HM550
HM550 Series
Specimen orientation X- and Y-axes universal 8° with zero posi-
tioning
Z-axis up to 360°
Coarse feed motorized, graduated, single
and continuous
Cooling chamber illumi-
nation with variable illumination position
Sliding window heated
Option M
Cutting drive manual and motorized, elect-
ronically controlled
Cutting window cutting window adjusted to
specimen size manually
Operating modes interval, single and conti-
nuous stroke
Cutting speed 0 to 250 mm/s
Handwheel brake electromagnetic, in any
position
Emergency stop via handswitch or foot pedal
Option V max. specimen size 28 mm
max. section thickness to be
disposed of max. 40 µm
max. section thickness to be
stretched 20 µm
min. section thickness to be
stretched 1 µm
Clearance angle adjust-
ment Standard knife carrier 5 to 16°
Disposable blade carrier 5 to 16°
Magnetic disposable blade
carrier 25 to 30°
Coarse filter Glass fiber reinforced
extraction thimble
Micro filter Borosilicate micro fiber 99.99% filtration efficiency
for 0.1 µm particles
Cold D (Option DC)
Max. filling amount ultrasonic bath 125 ml
Max. filling amount store reservoir disinfection
medium 1000 ml
(Recommended medium) Sanosil®
Introduction
Technical data HM550 Series

18 Thermo Fisher Scientific
Operation Manual HM550
HM550 Series
Frequency Ultrasonic-unit 1.63 MHz + 70 kHz
Transportation and storage
conditions
Storage temperature range -20°C to +50°C
Operating conditions +5°C up to +35°C (at a max.
rel. humidity of 60%)
altitude up to 2000 m M.S.L.
for indoor use only
Floor loading requirements:
300 kg/m2
Power requirements 100 V/50 Hz, 12 A, +/-10%
100 V/60 Hz, 12 A, +/-10%
115 V/60 Hz, 12 A, +/-10%
220...230 V/50 Hz, 6 A,
+/-10%
220 V/60 Hz, 6 A, +/-10%
240 V/50 Hz, 6 A, +/-10%
Internal protection
Primary circuits
Transformer T8AH, 100...115 V
T4AH, 220...240 V
Illumination T0,8AH, 100...115 V
T0,4AH, 220...240 V
Secondary circuits
Window heating T2.5AH
Vacutome valve (optional) T2AH
Pollution degree 2
Protection category I
Overvoltage category II
Sound pressure 45 dB(A)
measured with 1m distance to
the instrument
Fluids and gases cooling agent R404a, filling amount: 230 g
(230 V/50 Hz)
R404a, filling amount: 250 g
(115 V/60 Hz)
Dimensions Wide (w/o hand wheel 640 mm, deep: 760 mm, high:
1200 mm
Weight 125 to 155 kg (depending on
the model)
All temperatures refer to an ambient temperature of +20°Cꢞ
Introduction
Technical data HM550 Series

19Thermo Fisher Scientific Operation Manual HM550
Figure 2. Touchpad Keyboard with buttons and LC-display.
[1]ꢀ Onꢀindicationꢀ(sleepꢀstatus)ꢀ ꢀꢀ
[2]ꢀ Buttonꢀspecimenꢀtemperatureꢀꢀ
ꢀ (Standardꢀversionꢀnotꢀused,ꢀasꢀthereꢀisꢀnoꢀspecimenꢀcooling)
[3]ꢀ Buttonꢀchamberꢀtemperatureꢀ ꢀ
[4]ꢀ ButtonꢀTRIM/FEEDꢀ ꢀ
[5]ꢀ ArrowꢀbuttonꢀUPꢀ ꢀ
[6]ꢀ ArrowꢀbuttonꢀDOWNꢀ ꢀ
[7]ꢀ LCꢀdisplayꢀ ꢀ
[8]ꢀ Resetꢀbuttonꢀ ꢀ
[9]ꢀ Menuꢀbuttonꢀ ꢀ
[10]ꢀenterꢀbuttonꢀ(withinꢀtheꢀmenuꢀsettings)ꢀ
ꢀ scrollꢀbuttonꢀ(outsideꢀtheꢀmenuꢀsettings)
[11]ꢀInformationꢀlineꢀ ꢀ
[12]ꢀStatusꢀlineꢀ ꢀ
Note:
is operation manual includes all options. If your instrument is not
equipped with all options, some of the buttons might be disabled.
[5][4] [6] [11] [12]
[7][3][2][1] [8]
[10][9]
Introduction
Technical data HM550 Series
Information line
Status line
OMVPD

20 Thermo Fisher Scientific
Operation Manual HM550
Figure 3. Keypad left.
[1]ꢀ Buttonꢀcuttingꢀwindowꢀ ꢀꢀ
[2]ꢀ ButtonꢀACAꢀ ꢀ
[3]ꢀ Buttonꢀcoarseꢀfeed,ꢀforwardsꢀ ꢀ
[4]ꢀ Buttonꢀcoarseꢀfeed,ꢀbackwardsꢀ ꢀ
[5]ꢀ ButtonꢀTRIM
[4]
[3]
[1]
[2]
[5]
Introduction
Technical data HM550 Series