Thermo Scientific CryoStar NX70 series Manual

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COMPANY INFORMATION ...............................................................................................................................3
EMC STATEMENT ................................................................................................................................................... 4
IMPORTANT NOTE REGARDING INSTRUMENT VARIANTS ................................................................................5
SYMBOLS .........................................................................................................................................................6
TABLE OF CONTENTS........................................................................................................................................7
CHAPTER 1 - SAFETY INFORMATION ..............................................................................................................10
GENERAL SAFETY................................................................................................................................................... 10
CHEMICAL SAFETY ................................................................................................................................................. 11
ENVIRONMENT ..................................................................................................................................................... 11
WARRANTY STATEMENT ......................................................................................................................................... 12
CHAPTER 2 - INTRODUCTION .........................................................................................................................13
INTENDED USE...................................................................................................................................................... 13
INSTRUMENT FEATURES.......................................................................................................................................... 13
TECHNICAL SPECIFICATIONS..................................................................................................................................... 14
Mechanical Specifications............................................................................................................................ 14
Electrical Specifications................................................................................................................................ 14
Environmental Specifications ....................................................................................................................... 14
Cooling Specifications .................................................................................................................................. 15
Microtome and Sectioning Specifications .................................................................................................... 15
Cold D Specification...................................................................................................................................... 16
Default Settings............................................................................................................................................ 16
CHAPTER 3 - INSTRUMENT SETUP ..................................................................................................................18
UNPACKING AND REPACKING................................................................................................................................... 18
Safety Precautions ....................................................................................................................................... 18
Unpacking Procedure................................................................................................................................... 18
Repacking the Instrument ............................................................................................................................ 19
LOCATION &SETUP ............................................................................................................................................... 20
IDENTIFICATION OF PARTS....................................................................................................................................... 21
Overview ...................................................................................................................................................... 21
Connections Panel........................................................................................................................................ 22
Cryochamber................................................................................................................................................ 23
Blade Holder................................................................................................................................................. 24
Specimen Head NX70 ................................................................................................................................... 25
SET UP................................................................................................................................................................ 26
Fitting or Exchanging the Anti Roll Plate...................................................................................................... 26
Fitting or Exchanging Low Profile Blades ..................................................................................................... 27
Removing Used Blades with the Magnetic Blade Tool................................................................................. 28
Blade Positioning and Orientation ............................................................................................................... 29
Adjusting the Cutting Angle ......................................................................................................................... 30
Adjusting the Anti-Roll Plate ........................................................................................................................ 32
SPECIMEN SETUP .................................................................................................................................................. 33
Specimen Orientation .................................................................................................................................. 33
FILLING WITH DISINFECTANT TO PERFORM THE COLD DFUNCTION ................................................................................. 35

Filling the Cryostat with Disinfectant Solution (Sanosil®) ............................................................................ 35
CHAPTER 4 –OPERATION...............................................................................................................................37
SWITCHING ON..................................................................................................................................................... 37
SYSTEM INTERFACING............................................................................................................................................. 38
Using the Touchscreen ................................................................................................................................. 38
Main Screen ................................................................................................................................................. 38
SETTING PREFERENCES ........................................................................................................................................... 40
Quick Freezing of the Specimen ................................................................................................................... 40
Trimming and Fine Section Thickness .......................................................................................................... 41
Temperature ................................................................................................................................................ 43
Set the Blade Temperature .......................................................................................................................... 44
Cutting Window ........................................................................................................................................... 45
Select a Favourite......................................................................................................................................... 46
Vacutome and Cleaning Mode..................................................................................................................... 47
Height Adjustment ....................................................................................................................................... 51
Multi-Function Bar ....................................................................................................................................... 52
Time and Date.............................................................................................................................................. 52
Cutting Mode Selection................................................................................................................................ 53
Handwheel Brake ......................................................................................................................................... 58
Multi-Function Button.................................................................................................................................. 59
Menu............................................................................................................................................................ 61
Chamber Light.............................................................................................................................................. 62
Standby ........................................................................................................................................................ 63
Display Lock.................................................................................................................................................. 64
Favourites .................................................................................................................................................... 65
User Guide.................................................................................................................................................... 67
Settings Menu .............................................................................................................................................. 69
Language ..................................................................................................................................................... 70
Time and Date.............................................................................................................................................. 71
Start Time..................................................................................................................................................... 74
Defrost ......................................................................................................................................................... 76
Immediate Defrosting .................................................................................................................................. 76
Programming the Daily Defrost Cycle .......................................................................................................... 78
Screen........................................................................................................................................................... 80
Retraction .................................................................................................................................................... 81
Data Log....................................................................................................................................................... 83
Default Settings............................................................................................................................................ 84
Service .......................................................................................................................................................... 85
EMERGENCY STOP ................................................................................................................................................. 86
Standard Emergency Stop Function ............................................................................................................. 86
Optional Foot Pedal Emergency Stop........................................................................................................... 87
COOLING PROCESSES ............................................................................................................................................. 87
Specimen and blade holder cooling ............................................................................................................. 87
SECTIONING PROCEDURES....................................................................................................................................... 88
Cutting Movement and Retraction .............................................................................................................. 88
Trimming and First Cuts ............................................................................................................................... 89
Trimming...................................................................................................................................................... 89
Sectioning and Taking Off Sections .............................................................................................................. 90

HANDWHEEL BRAKES ............................................................................................................................................. 91
Electronic Handwheel Brake ........................................................................................................................ 91
Mechanical Handwheel Brake ..................................................................................................................... 92
COLD D............................................................................................................................................................... 93
Starting the Disinfection (Cold D) Function .................................................................................................. 93
Aborting the Disinfection ............................................................................................................................. 95
Programming Cold D.................................................................................................................................... 96
CHAPTER 5 - TROUBLESHOOTING ..................................................................................................................98
TROUBLESHOOTING TABLES..................................................................................................................................... 98
TIPS FOR SUCCESSFUL SECTIONING ......................................................................................................................... 100
How to avoid malfunctions when working with the Vacutome ................................................................. 101
Sectioning Temperature Guidelines for Fresh Tissues ................................................................................ 102
CHAPTER 6 - CLEANING AND MAINTENANCE ...............................................................................................103
CLEANING THE TOUCH SCREEN DISPLAY................................................................................................................... 103
SHUTTING OFF FOR CLEANING ............................................................................................................................... 103
CLEANING AND CARE OF THE BLADE HOLDER ............................................................................................................103
EMPTYING THE DEFROSTING AND COLD DLIQUIDS ....................................................................................................104
EXCHANGE THE VACUTOME FILTER BAG .................................................................................................................. 105
ADJUSTMENT OF THE MOVABLE SPECIMEN HEAD JAW ............................................................................................... 107
TRANSPORTATION OF THE INSTRUMENT................................................................................................................... 108
Conditions for the Transportation of the Instrument................................................................................. 108
APPENDICES.................................................................................................................................................110
APPENDIX A-SPARES AND ACCESSORIES .................................................................................................................110
APPENDIX B-RECOMMENDED DISINFECTION PROCEDURE .......................................................................................... 112
APPENDIX C-COLD DISINFECTION CERTIFICATE ........................................................................................................113
INDEX ..........................................................................................................................................................114

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