Thermo Scientific CryoStar NX70 series Manual

Thermo Scientific CryoStar NX70
Product Code
956960, 956970, 956980, 956990, 957000, 957010,
957020, 957030, 957040, 957050, 957060, 957070
Operator Guide
387928 Issue 10

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Company Information
© Copyright 2016. Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific Inc. (Thermo Fisher Scientific) is the world leader in serving science, offering a
unique combination of innovative technologies. Thermo Scientific is a brand name of Thermo Fisher
Scientific.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific makes every attempt to ensure that the information contained in this supporting
document is correct and clearly stated, but does not accept responsibility for any errors or omissions. The
development of Thermo Scientific products and services is an ongoing process. Please ensure that any
published information you use as a reference is up to date and relates to the condition of the product. If
necessary, check with your local Thermo Fisher Scientific representative.
This document may not, in whole or in part, be copied, photocopied, reproduced, translated, or
converted to any electronic or other form without prior written consent of Thermo Fisher Scientific. All
information contained in this manual is proprietary and confidential, and the exclusive property of
Thermo Fisher Scientificand is protected by copyright.
Contact Addresses:
Thermo Shandon Limited (Trading as Thermo Fisher Scientific),
Tudor Road, Manor Park,
Runcorn, WA7 1TA, UK
Tel: [phone]; Fax: [phone]
Email: [e-mail]
Web: http://www.thermoscientific.com/pathology
USA Distributor:
Richard-Allan Scientific
Subsidiary of Thermo Fisher Scientific
4481 Campus drive
Kalamazoo
MI 49008
USA
This instrument conforms to the essential requirements of:
IVD Directive 98/79/EC
and its harmonized standards under Union harmonization legislation

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NX70 110V
EC Regulation No 517/2014 – Fluorinated Greenhouse Gas Regulations
Conta ins fluorina ted greenhouse ga ses
Refrigerant: R404A
R404A GWP value = 3992
System charge weight = 0.400 Kg
System CO2 equiva lent cha rge weight = 1.597 tonnes
NX70 230V
EC Regulation No 517/2014 – Fluorinated Greenhouse Gas Regulations
Conta ins fluorina ted greenhouse ga ses
Refrigerant: R404A
R404A GWP value = 3992
System charge weight = 0.400 Kg
System CO2 equiva lent cha rge weight = 1.597 tonnes
EMC Statement
This IVD equipment complies with the emissions and immunity requirements of IEC 61326-2-6:2012
and IEC 61326-1:2012.
This equipment has been designed and tested to CISPR 11:2009+A1:2010 Class A.
It is intended for use in a laboratory environment by a trained and qualified professional. In a domestic
environment it may cause radio interference, in which case it may be necessary to take measures to
mitigate the interference.

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Important Note Regarding Instrument Variants
This document is designed for use with all variants of the Thermo Scientific CryoStar NX70 cryostat.
A range of optional features are available on this instrument and the instructions contained in this manual
are not specific to any one variant. Thus some features described may not be available on your instrument.
This IFU is valid for the variants listed below:
Product Code Product Variant
956960 CryoStar NX70 HOMP 100V 60 Hz
956970 CryoStar NX70 HOMPD 100V 60 Hz
956980 CryoStar NX70 HOMPV 100V 60 Hz
956990 CryoStar NX70 HOMPDV 100V 60 Hz
957000 CryoStar NX70 HOMP 110-120V 60 Hz
957010 CryoStar NX70 HOMPD 110-120V 60 Hz
957020 CryoStar NX70 HOMPV 110-120V 60 Hz
957030 CryoStar NX70 HOMPDV 110-120V 60 Hz
957040 CryoStar NX70 HOMP 220-230V 50/60Hz
957050 CryoStar NX70 HOMPD 220-230V 50/60Hz
957060 CryoStar NX70 HOMPV 220-230V 50/60Hz
957070 CryoStar NX70 HOMPDV 220-230V 50/60Hz

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Symbols
The following symbols and conventions may be used throughout this document and on the instrument:
This symbol is used on the instrument, or in a document, to indicate that instructions
must be followed for safe and correct operation. If this symbol appears on the
instrument, always refer to the operator guide.
This symbol is used on the instrument, or in a document, to indicate that there are
potential biological risks associated with the instrument and / or instrument use. Always
use Good Laboratory Practice.
Cold surface, if necessary, use gloves
Cutting hazard, sharp edges, watch your fingers.
This symbol is used on the instrument, or in a document, to indicate that irritants or
potentially harmful chemicals are present. Refer to the Material Safety Data Sheets for
the products, and always use Good Laboratory Practice.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European
countries with a separate collecting system within the waste management. This product,
being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).
Manufacturer.
Serial number
It is stated on the product label sticker.
It is built up as follows: SYYMMXXXX
S=Production site, Y=year of production, M=Month of production, X= Counter
So the serial number states the production date of the device
A warning is given in the documentation if there is a danger of personal injury or damage to the
equipment or samples.
Note
Notes give additional information about a job or instruction, but do not form part of the instruction.
SN

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Table of Contents
COMPANY INFORMATION............................................................................................................................................ 3
EMC STATEMENT .......................................................................................................................................................... 4
IMPORTANT NOTE REGARDING INSTRUMENT VARIAN TS ....................................................................................... 5
SY MBOL S........................................................................................................................................................................ 6
TABL E OF CONTEN TS..................................................................................................................................................... 7
CHAPTER 1 - SAFETY INFORMATION.........................................................................................................................10
GENERAL SAFETY..........................................................................................................................................................10
CHEMICAL SAFETY ........................................................................................................................................................11
ENVI RONMENT.............................................................................................................................................................11
WARRANTY STATEMENT................................................................................................................................................12
CHAPTER 2 - INTRODUCTION.....................................................................................................................................13
INTENDED USE.............................................................................................................................................................13
INSTRUMENT FEATURES ................................................................................................................................................13
TECHNICAL SPECIFICATIONS...........................................................................................................................................14
Mechanical Specifications..................................................................................................................................14
Electrical Specifications......................................................................................................................................14
Environmental Specifications.............................................................................................................................14
Cooling Specifications.........................................................................................................................................15
Microtome and Sectioning Specifications.........................................................................................................15
Cold D Specification............................................................................................................................................16
Default Settings..................................................................................................................................................16
CHAPTER 3 - INSTRUMEN T SETUP.............................................................................................................................18
UNPACKING A ND REPACKING .........................................................................................................................................18
Safety Precautions..............................................................................................................................................18
Unpacking Procedure.........................................................................................................................................18
Repacking the Instrument..................................................................................................................................19
LOCATION &SETUP ......................................................................................................................................................20
IDENTIFICATION OF PARTS .............................................................................................................................................21
Overview..............................................................................................................................................................21
Connections Panel..............................................................................................................................................22
Cryochamber.......................................................................................................................................................23
Blade Holder........................................................................................................................................................24
Specimen Head NX70.........................................................................................................................................25
SET UP........................................................................................................................................................................26
Fitting or Exchanging the Anti Roll Plate..........................................................................................................26
Fitting or Exchanging LowProfile Blades..........................................................................................................27
Removing Used Blades with the Magnetic Blade Tool.....................................................................................28
Blade Positioning and Orientation....................................................................................................................29
Adjusting the Cutting Angle...............................................................................................................................30
Adjusting the Anti-Ro ll Plate..............................................................................................................................32
SPECIMEN SETUP..........................................................................................................................................................33
Specimen Orientation.........................................................................................................................................33
FILLING WITH DISINFECTANT TO PERFORM THE COLD DFUNCTI ON .....................................................................................35

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Filling the Cryostat with Disinfectant Solution (Sanosil®)................................................................................35
CHAPTER 4 – OP ERA TION ...........................................................................................................................................37
SWITCHING ON............................................................................................................................................................37
SYSTEM INTERFACING ...................................................................................................................................................38
Using the Touchscreen.......................................................................................................................................38
Main Screen ........................................................................................................................................................38
SETTING PREFERENCES ..................................................................................................................................................40
Quick Freezing of the Specimen.........................................................................................................................40
Trimming and Fine Section Thickness................................................................................................................41
Temperature.......................................................................................................................................................43
Set the Blade Temperature................................................................................................................................44
Cutting Window..................................................................................................................................................45
Select a Favourite...............................................................................................................................................46
Vacutome and Cleaning Mode..........................................................................................................................47
Height Adjustment..............................................................................................................................................51
Multi-Fun ction Bar..............................................................................................................................................52
Time and Date.....................................................................................................................................................52
Cutting Mode Selection......................................................................................................................................53
Handwheel Brake................................................................................................................................................58
Multi-Function Button........................................................................................................................................59
Menu ...................................................................................................................................................................61
Chamber Light.....................................................................................................................................................62
Standby................................................................................................................................................................63
Display Lock.........................................................................................................................................................64
Favourites............................................................................................................................................................65
User Guide...........................................................................................................................................................67
Settings Menu.....................................................................................................................................................69
Language.............................................................................................................................................................70
Time and Date.....................................................................................................................................................71
Start Time............................................................................................................................................................74
Defrost.................................................................................................................................................................76
Immediate Defrosting ........................................................................................................................................76
Programming the Daily Defrost Cycle...............................................................................................................78
Screen ..................................................................................................................................................................80
Retraction............................................................................................................................................................81
Data Log..............................................................................................................................................................83
Default Settings..................................................................................................................................................84
Service .................................................................................................................................................................85
EMERGENCY STOP ........................................................................................................................................................86
Standard Emergency Stop Function..................................................................................................................86
Optional Foot Pedal Emergency Stop................................................................................................................87
COOLING PROCESSES ....................................................................................................................................................87
Specimen and blade holder cooling...................................................................................................................87
SECTIONING PROCEDURES .............................................................................................................................................88
Cutting Movementand Retraction....................................................................................................................88
Trimming and First Cuts.....................................................................................................................................89
Trimming.............................................................................................................................................................89
Sectioning and Taking Off Sections...................................................................................................................90

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HANDWHEEL BRAKES ....................................................................................................................................................91
Electronic Handwheel Brake..............................................................................................................................91
Mechanical Handwheel Brake...........................................................................................................................92
COLD D.......................................................................................................................................................................93
Starting the Disinfection (Cold D) Function.......................................................................................................93
Aborting the Disinfection ...................................................................................................................................95
Programming Cold D..........................................................................................................................................96
CHAPTER 5 - TROUBLESHOOTING..............................................................................................................................98
TROUBLESHOOTING TABLES ...........................................................................................................................................98
TIPS FOR SUCCESSFUL SECTIONING ...............................................................................................................................100
How to avoid malfunctions when working with the Vacutome....................................................................101
Sectioning Temperature Guidelines fo r Fresh Tissu es....................................................................................102
CHAPTER 6 - CLEANING AND MAINTENANC E.........................................................................................................103
CLEANI NG THE TOUCH SCREEN DISPLAY ........................................................................................................................103
SHUTTING OFF FOR CLEANING .....................................................................................................................................103
CLEANI NG AND CARE OF THE BLADE HOLDER..................................................................................................................103
EMPTYING THE DEF ROS TING AND COLD DLIQUIDS.........................................................................................................104
EXCHANGE THE VACUTOM E FILTER BAG ........................................................................................................................105
ADJUSTMENT OF THE MOVABLE SPECIMEN HEAD JAW....................................................................................................107
TRANSPORTATION OF THE INS TRUMENT.........................................................................................................................108
Conditions for the Transportation of the Instrument.....................................................................................108
APPENDICES...............................................................................................................................................................110
APPENDIX A-SPARES AND ACCESSORIES.......................................................................................................................110
APPENDIX B-RECOMMENDED DISINFECTION PROCEDURE ..............................................................................................112
APPENDIX C-COLD DISINFECTIONCERTIFICATE.............................................................................................................113
INDEX..........................................................................................................................................................................114

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Chapter 1 - Safety Information
Thermo Scientific instruments are designed for convenient and reliable service; however, improper use or
handling by a user may damage the instrument. Protection might be impaired if the equipment is used in
a manner not specified by the manufacturer. Correct maintenance procedures are essential for consistent
performance. It is recommended that users secure a maintenance contract with our service department
acting on behalf of Thermo Shandon Limited.
Any problems and queries should be referred to your Thermo Fisher Scientific service department.
The following sections contain important information for the safe setup and use of the
instrument, and should be read and understood by the user before using the instrument.
General Safety
This instrument, as supplied, conforms to IEC 61010-1 and IEC 61010-2-
101; however, the
addition of chemicals introduces potential hazards. Good Laboratory Practice must be
employed and consideration must be given to the potential for hazard when dealing with
these chemicals.
Do not use the instrument in close proximity to strong electromagnetic radiation, as these
may interfere with the proper operation. The electromagnetic environment should be
evaluated prior to operation of the device.
Good Laboratory Practice must be used when handling tissue samples to prevent cross
contamination and infection. The user should complete a risk assessment to determine any
potential hazards related to tissue handling.
Do not introduce any source of ignition into, or near, the instrument once it has been loaded
with reagents.
Do not remove any panels or access covers, unless specifically instructed to do so. The
instrument does not have any user serviceable parts. Potentially lethal voltages are present
inside the instrument.
The instrument must be properly connected to a good earth (ground) via the Mains input
supply and positioned such that it is possible to interrupt the Mains supply at the source by
removing the plug from the socket.
Use only factory approved accessories or replacement parts within the instrument.
Only use reagents recommended in the operatorguide.

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Chemical Safety
The introduction of chemicals creates potential hazards. Thermo Fisher Scientific has adopted the
following position with regard to the subject of volatile chemicals used in laboratories:
•Customers using non-specified chemicals in the instrument, do so at their own
risk.
•All chemicals recommended by Thermo Fisher Scientific have auto-ignition
temperatures considerably above any surface temperatures that can be reached
during a single fault failure on the instrument.
•The instrument contains no source o
f ignition in any areas of the instrument where
chemicals are stored, or likely to leak into, in a single fault condition.
•
The operator is fully aware of the contents of the specification documents detailing
the properties of the chemicals they are using.
•The operator has carried out any legally required assessment of chemicals used and
is using Good Laboratory Practice.
Environment
This instrument complies with the European Union's Waste Electrical and Electronic Equipment
(WEEE) Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific on behalf of Thermo Shandon Limited has contracts with one or more recycling
/ disposal companies in each EU Member State, and this product and packaging should be disposed of or
recycled through them. For further information contact your Thermo Fisher Scientificservice
representative.

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Warranty Statement
Thermo Shandon Limited as part of Thermo Fisher Scientific is proud of their quality, reliability and of
our after-sales service. We continuously strive to improve our service to our customers.
Please ask your distributor or Thermo Fisher Scientific representative about service contracts which can
help maintain your instrument in an optimal operating condition.
Warranty provisions necessarily vary to comply with differences in national and regional legislation.
Specific details can be found in the delivery documentation or from your dealer or representative.
Please note that your warranty may be invalidated if:
•This instrument is modified in any way, or not used as intended by The rmo Shandon Limited.
•Accessories and reagents which have not been approved by Thermo Fisher Scientific are used.
•The instrument is not operated or maintained in accordance with instructions.

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Chapter 2 - Introduction
Intended Use
The Thermo Scientific cryostat NX70 is an in vitro diagnostic device, designed to take
precision sections of tissue specimens including the sectioning of paraffin embedded
samples in medical, pharmaceutical laboratories as necessary preparation for their
examination. Only qualified and trained laboratory personnel may operate the NX70.
The instrument may only be operated within the scope of its intended use as described above and as per
the instructions given in this manual.
Any other use of this instrument is considered as improper operation.
Instrument Features
The CryoStar NX70 is a precision-engineered, ergonomically-designed, high-performance cryostat in
modular design with a stainless steel cooling chamber. Some of its features are:
•Knife / Blade Holder cooling.
•Specimen cooling.
•Ergonomic height adjustment with integrated ergonomic casing.
•Colour touch-screen user interface and joystick control.
•Motorized cutting with variable speed control (from 0 to 256 mm/s).
•Optional Cold D function consisting of an internal fumigation unit that applies Sanosil®
(disinfection medium) into the user-accessible, operating areas of the cryochamber.
•Optional Vacutome function.

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Technical Specifications
Mechanical Specifications
Depth 100 cm (39.4 in)
Width 75.5 cm (29.7 in)
Height Range Adjustable range 82 - 112 cm (32.3 - 44.0 in)
Maximum Weight 200 kg (440 lb)
Electrical Specifications
Power Requirements Dedicated line recommended.
100V, 50/60Hz, 10A, +/-10%, 110-120V, 60Hz, 10A, +/-10%
220-230V, 50/60Hz, 5A, +/-10%
Environmental Specifications
For Indoor Use Only
Temperature (Operating
Limits)
+5°C to +35°C (+41°F to +95°F)
Temperature
(Recommended
Operation)
+15°C to +30°C (+59°F to +86°F)
Note:
Performance may deteriorate when operated outside of this
range.
Temperature
(Transport and Storage)
-20°C to +40°C (-4°F to 122°F)
+70°C (158°F) for short exposure
Relative Humidity Max. 80% RH up to 31°C
Decreasing linearly to 50% RH at 40°C
Altitude Up to 2000 m (6,500 ft)
Pollution Degree 2
Over Voltage Category II
Floor Loading
Requirements
300 kg/m² (660 lb/ft²)
Protection Class I
Sound Emission <45 dB (A) - measured with 1m distance to the instrument.
Refrigerant R404a, filling amount 400g

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Cooling Specifications
Specimen Temperature
Control
-50°C to +10°C (-58°F to +50°F) at +20°C room Temp. (decrease
0,7°C/1°C room temp. increase)
Blade Temperature
Control
-35°C to -5°C (-31°F to +23°F) at 20°C room Temp (decrease 0.5°C
71°C room temp. increase)
Cryobar Cooling Max. -60°C (-76°F) at -35°C blade temperature
Defrosting Options Programmed / Immediate
Microtome and Sectioning Specifications
Section Thickness Range 0.5 – 500 µm
Fine Section Thickness
Range
0.5 - 100 µm
Fine Section Thickness
Resolution
0.5 – 2 µm in 0.5 µm steps
2 µm – 10 µm in 1 µm steps
10 µm – 20 µm in 2 µm steps
20 µm – 50 µm in 5 µm steps
50 µm – 100 µm in 10 µm steps
Trimming Thickness
Range
5 - 500 µm
Trimming Thickness
Resolution
5 – 10 µm in 5 µm steps
10 µm – 100 µm in 10 µm steps
100 µm – 200 µm in 20 µm steps
200 µm – 500 µm in 50 µm steps
Specimen Retraction
(Return Travel)
20 µm
Vertical Specimen Stroke 64 mm
Horizontal Specimen
Movement
48 mm
Specimen Approach Manual (variable speed by joystick control), maximum speed: 3mm/s
Chuck Size 30, 40 mm (additional special sizes are available)
Specimen Orientation x - and y - axes universal 7° with zero positioning; z - axis up to 360°

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Coarse Feed Motorized
Cutting Drive Motorized, electronically controlled and manual via handwheel
Cutting Window Manually adjusted to specimen size
Operating Modes Interval, single, multi- and continuous-stroke
Cutting Speed 0 - 256 mm/s (0-120 1/min)
Clearance Angle
Adjustment
Disposable blade carrier 8- 16°
Vacutome Filter approximately 500 ml
Cold D Specification
Reservoir Volume 1000 ml
Recommended Medium Sanosil®S010 (5% H2O2)
Default Settings
Counter Mode Speed V = 0
Multi Counter 2
Operating Mode Continuous
Retraction ON
Language English
Fine 0.5 µm
Trim 5 µm
Joystick Calibration Calibrated
Chamber Light 100%
Screen Brightness 100%
Brake ON
Stepper Motor Initialization Yes
Blade Temperature -15°C
Specimen Temperature -15°C
Cutting Window 0 mm (OFF)
Favorites (Program1, Program 2,
Program 3)
(-15°C/-15°C), (-20°C/-20°C), (-25°C/-25°C)
Cryobar OFF
Vacutome OFF
Disinfection OFF

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Disinfection, Cycle (Automatic) ON (03:00 Night)
Defrost Cycle (Automatic) ON (00:00 Night)
Standby Mode 1h (After 1 hour, the Cryostat enters the Standby Mode
automatically). Kept temperature: -15°C.
Sleep Mode 3h (After 3 hours, the Cryostat enters the Sleep Mode
automatically). Kept temperature: -10°C. Power savings:
Approximately 30%.
Start Time 06:00 a.m.
Section Counter 0
Section Sum 0

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Chapter 3 - Instrument Setup
Unpacking and Repacking
Safety Precautions
The CryoStar NX70 is heavy and requires a minimum of two people to safely unpack and
manoeuvre it.
The CryoStar NX70 has a high centre of gravity and care should be taken when moving.
Do not tip the CryoStar NX70more than 30° from the upright position.
After moving the CryoStar NX70, wait at least 8 hours before switching on to allow the
refrigerant to settle - failure to do so may cause damage to the unit.
Note
The packing materials should be carefully stored for use in any future instrument shipment.
Unpacking Procedure
General Information:
•Ensure that these Instructions are retained for future use.
•The CryoStar NX70 is delivered on a pallet. The sides of the instrument are encased in cardboard
and cushioned with foam spacers inside the container. A wooden cover protects the top of the
instrument.
•In unpacking be aware of the weight (200 Kg/440 lbs) of the instrument.
Safe handling of the installation requires the involvement of two people
Handling
:
•Place the crate on a flat surface while ensuring that there are no objects underneath the pallet.
•Remove the wooden cover form the crate but retain it for later use as a ramp.
•Remove the top foam spacer from the crate.
•Remove the exteriorcardboard container by opening the clips.
•Remove the remaining foam spacers and the accessories carton. Place all the accessories carefully
to one side.
•Remove the plastic cover from the instrument.
•Two wing-nuts are now visible at the front edges of the wooden pallet.

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•Using the wing-nuts affix the wooden top of the crate to the pallet to serve as an unloading ramp
for the instrument.
•The instrument can be moved on its castors. However, ensure that it does not roll off the pallet
inadvertently.
•Screw in the two bails (part of the standard accessories) into the respective holes in the foot of the
cryostat.
•Mount the transport belts (part of the standard accessories) with the bails.
To move the instrument from the pallet stand BEHIND (NOT in front) of the
instrument.
•Carefully roll the instrument off the pallet and on to the floor.
•Move the instrument to the desired location while leaving space on the left- and right-hand.
•Keep all packing and transportation materials together in case of future need.
•In the future, if the instrument is transported by forklift, truck, train, ship or aircraft it must be
packed in the original shipping container with all transportation locks in place.
Repacking the Instrument
•To repack the instrument, follow the above instructions in reverse order.

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Location & Setup
When deciding where to locate the CryoStar NX70, the following items must be taken into
consideration:
•Approximately 10 cm (4 in) must be allowed around the cooling system to allow free air flow.
•Approximately 10 cm (4 in) must be allowed between the wall and the rear panel.
•The vents on the sides of the rear panel must be unobstructed at all times.
•The mains power socket must be accessible at all times.
•The location must be free from draughts, open doors or air conditioning systems.
•The location must not expose the cryochamber to direct sunlight.
•Enough room for height adjustment.
Note
These measures are intended to reduce the build-up of frost and maintain consistent cooling temperatures
resulting in more favourable working conditions. Locations which have high humidity and / or high
ambient temperatures can reduce the performance of the instrument.
Once the CryoStar NX70 has been located, ensure the fixing bolts shown are tightened to
prevent the unit from moving during operation.