ThermoFisher Scientific Vanquish VH-D10 User manual

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Vanquish
Diode Array
Detectors
VH-D10
OperatingManual
4820.8301‐ENRevision2.0a•September2017
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thermoscientific

Copyright © 2017 Thermo Fisher Scientific Inc. All rights reserved.
Original Operating Manual
The hardware descriptions in this manual revision refer to detectors VH-D10. For the manual
release history, see page 188.
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Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to
use in the product operation. The document is copyright protected; any reproduction of the
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of Thermo Fisher Scientific Inc.
This manual is provided "as is." The contents of this manual are subject to being changed,
without notice, in future revisions.
Thermo Fisher Scientific Inc. makes no representations that this document is complete,
accurate, or error-free. Thermo Fisher Scientific Inc. assumes no responsibility and will not be
liable for any errors, omissions, damage, or loss that might result from any use of this document,
even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a
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The Terms and Conditions of Sale shall govern all conflicting information between the two
documents.
Printed manual version only
Printed in Germany on 100% chlorine-free bleached, high-white paper that is produced in an
environmentally friendly process, leading to a paper profile of zero CO2emissions.
Manufacturer's address
Dionex Softron GmbH, Part of Thermo Fisher Scientific
Dornierstrasse 4, D-82110 Germering

Contacting Us
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 3
Contacting Us
There are several ways to contact us:
Ordering Information
For ordering information or sales support for HPLC products, contact
your local Thermo Fisher Scientific sales organization. For contact
information, go to Contact Us on http://www.thermofisher.com.
Technical Assistance
For technical support for HPLC products, contact your local Thermo
Fisher Scientific support organization. For contact information, go to
Contact Us on http://www.thermofisher.com.

Contacting Us
Page 4 Vanquish Diode Array Detectors (VH-D10) Operating Manual

Contents
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 5
Contents
1 Using this Manual ............................................................................ 9
1.1 About this Manual ................................................................................................10
1.2 Conventions ..........................................................................................................11
1.2.1 Safety Messages.......................................................................................11
1.2.2 Special Notices and Informational Notes.................................................11
1.2.3 Typographical Conventions......................................................................12
1.3 Reference Documentation....................................................................................13
2 Safety..............................................................................................15
2.1 Safety Symbols and Signal Words.........................................................................16
2.1.1 Safety Symbols and Signal Words in This Manual ...................................16
2.1.2 Observing this Manual .............................................................................17
2.1.3 Safety Symbols on the Detector ..............................................................17
2.1.4 Rating Plate ..............................................................................................18
2.2 Intended Use.........................................................................................................19
2.3 Safety Precautions ................................................................................................20
2.3.1 General Safety Information .....................................................................20
2.3.2 Qualification of the Personnel .................................................................21
2.3.3 Personal Protective Equipment ...............................................................21
2.3.4 Electrical Safety Precautions....................................................................22
2.3.5 General Residual Hazards ........................................................................23
2.3.6 In Case of Emergency...............................................................................25
2.4 Solvent and Additive Information.........................................................................26
2.5 Compliance Information .......................................................................................28
3 Detector Overview..........................................................................29
3.1 Detector Features.................................................................................................30
3.2 Operating Principle ...............................................................................................31
3.3 Interior Components.............................................................................................33
3.4 Flow Cell................................................................................................................34
3.5 Lamp .....................................................................................................................36
3.6 Leak Detection ......................................................................................................37
3.7 Operation..............................................................................................................38

Contents
Page 6 Vanquish Diode Array Detectors (VH-D10) Operating Manual
4 Unpacking.......................................................................................39
4.1 Unpacking ............................................................................................................ 40
4.2 Scope of Delivery ................................................................................................. 43
5 Installation......................................................................................45
5.1 Safety Guidelines for Installation......................................................................... 46
5.2 Installing the Detector ......................................................................................... 47
5.3 Site Requirements................................................................................................ 49
5.4 Accessing the Interior Components..................................................................... 51
5.5 Setting Up the Hardware ..................................................................................... 52
5.5.1 System Arrangement............................................................................... 52
5.5.2 Connecting the Detector......................................................................... 54
5.5.3 Installing the Flow Cell ............................................................................ 57
5.6 Setting Up the Flow Connections......................................................................... 62
5.6.1 General Information and Guidelines....................................................... 62
5.6.2 Guiding Capillaries and Tubing Through the System .............................. 63
5.6.3 Connecting Fittings, Capillaries, and Tubing ........................................... 64
5.6.4 Flow Connections to the Flow Cell.......................................................... 67
5.6.5 Guiding Liquid Leaks to Waste ................................................................ 72
5.7 Determining the Backpressure in the Flow Cell................................................... 73
5.7.1 Determining the Backpressure without LightPipe flow cell.................... 74
5.7.2 Determining the Backpressure of the LightPipe Flow Cell...................... 76
5.8 Turning On the Detector ...................................................................................... 78
5.9 Setting Up the Detector in the Software ............................................................. 79
6 Operation .......................................................................................81
6.1 Introduction to this Chapter ................................................................................ 82
6.2 Safety Guidelines for Operation .......................................................................... 83
6.3 Control Elements.................................................................................................. 84
6.3.1 Keypad..................................................................................................... 84
6.3.2 Status Indicators...................................................................................... 86
6.4 Power On/Off Control.......................................................................................... 88
6.5 Preparing the Detector for Operation ................................................................. 89
6.5.1 Guidelines for Use of LightPipe Flow Cells.............................................. 91
6.5.2 Turning On the Lamp............................................................................... 93

Contents
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 7
6.6 Important Operating Parameters .........................................................................94
6.7 Optimizing the Performance of the Detector.....................................................100
6.7.1 General Guidelines.................................................................................100
6.7.2 Overview of Optimization Parameters ..................................................101
6.7.3 Selecting the Data Collection Rate.........................................................102
6.7.4 Selecting the Response Time and Peak Width.......................................102
6.7.5 Optimizing the Bandwidth and Slit Width Settings................................103
6.7.6 Selecting the Reference Wavelength and Bandwidth ...........................104
6.8 Shutting Down the Detector...............................................................................106
6.8.1 Short-Term Shutdown (Interruption of Operation)...............................106
6.8.2 Long-Term Shutdown.............................................................................107
7 Maintenance and Service..............................................................111
7.1 Introduction to Maintenance and Service..........................................................112
7.2 Safety Guidelines for Maintenance and Service.................................................113
7.2.1 General...................................................................................................113
7.2.2 Flow Cells ...............................................................................................115
7.3 General Rules for Maintenance and Service ......................................................116
7.4 Routine and Preventive Maintenance ................................................................117
7.4.1 Maintenance Plan ..................................................................................117
7.4.2 Cleaning or Decontaminating the Detector...........................................118
7.4.3 Predictive Performance .........................................................................120
7.5 Performing a Wavelength Validation..................................................................121
7.6 Replacing the Lamp.............................................................................................123
7.7 Flow Cell..............................................................................................................126
7.7.1 Flushing the Flow Cell ............................................................................126
7.7.2 Removing the Flow Cell..........................................................................128
7.7.3 Installing the Flow Cell ...........................................................................130
7.8 Back-Flushing the Flow Cell ................................................................................134
7.9 Diagnostic Cell.....................................................................................................141
7.9.1 Installing the Diagnostic Cell..................................................................141
7.9.2 Removing the Diagnostic Cell ................................................................143
7.10 Replacing the Waste Line....................................................................................144
7.11 Replacing the Main Power Fuses ........................................................................146
7.12 Updating the Detector Firmware........................................................................148
7.13 Replacing the Doors............................................................................................150

Contents
Page 8 Vanquish Diode Array Detectors (VH-D10) Operating Manual
7.14 Transporting or Shipping the Detector .............................................................. 152
7.14.1 Preparing the Detector for Transport ................................................... 153
7.14.2 Transporting the Detector to a New Location ...................................... 154
7.14.3 Shipping the Detector ........................................................................... 155
7.15 Slide-In Module.................................................................................................. 157
7.15.1 Removing the Slide-In Module.............................................................. 157
7.15.2 Installing the Slide-In Module ............................................................... 159
8 Troubleshooting............................................................................163
8.1 General Information about Troubleshooting .................................................... 164
8.2 Messages............................................................................................................ 167
8.3 Checking the Flow Cell ....................................................................................... 171
8.4 Resolving Leakage .............................................................................................. 172
9 Specifications................................................................................173
9.1 Performance Specifications ............................................................................... 174
9.1.1 Detector ................................................................................................ 174
9.1.2 LightPipe Flow Cells............................................................................... 177
9.2 Physical Specifications ....................................................................................... 178
10 Accessories, Consumables and Replacement Parts......................179
10.1 General Information .......................................................................................... 180
10.2 Ship Kit ............................................................................................................... 181
10.3 Optional Accessories.......................................................................................... 182
10.4 Consumables and Replacement Parts ............................................................... 183
11 Appendix ....................................................................................185
11.1 Compliance Information .................................................................................... 186
11.1.1 Declarations of Conformity................................................................... 186
11.1.2 WEEE Compliance ................................................................................. 187
11.1.3 FCC Compliance..................................................................................... 188
11.1.4 NIST Compliance ................................................................................... 188
11.1.5 Manual Release History ........................................................................ 188
11.2 UV Cutoff Wavelengths of Solvents................................................................... 189
12 Index...........................................................................................191

1•Using this Manual
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 9
1Using this Manual
This chapter provides information about this manual, the conventions used
throughout the manual, and the reference documentation that is available
in addition to this manual.

1•Using this Manual
Page 10 Vanquish Diode Array Detectors (VH-D10) Operating Manual
1.1 About this Manual
This manual describes the functional features and operating principle of
your Vanquish™ detector and provides instructions for installation, set
up, start up, shut down, operation, maintenance and troubleshooting.
The layout of this manual is designed to provide quick reference to the
sections of interest to the user. To obtain a full understanding of your
detector, read this manual thoroughly.
This manual also contains safety messages, precautionary statements,
and special notices that can prevent personal injury, damage to the
detector, or loss of data when followed properly.
Note the following:
•The detector configuration may vary; therefore, not all descriptions
necessarily apply to your particular detector.
•If some detail applies to only one model or variant, the model or
variant is identified by name.
•Illustrations in this manual are provided for basic understanding.
They can vary from the actual model of the detector or component.
However, this does not influence the descriptions. No claims can be
derived from the illustrations in this manual.
The descriptions in this manual assume that the detector is installed in
the Vanquish system stack. If this is not the case, additional hardware is
required and must be ordered separately. The information in this
manual applies correspondingly.

1•Using this Manual
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 11
1.2 Conventions
This section describes the conventions that are used throughout this
manual.
1.2.1 Safety Messages
The safety messages and precautionary statements in this manual
appear as follows:
•Safety messages or precautionary statements that apply to the
entire manual and all procedures in this manual are grouped in the
Safety chapter.
•Safety messages or precautionary statements that apply to an entire
section or to multiple procedures in a section appear at the
beginning of the section to which they apply.
•Safety messages that apply to only a particular section or procedure
appear in the section or procedure to which they apply. They appear
different from the main flow of text.
Safety messages are often preceded by an alert symbol and/or alert
word. The alert word appears in uppercase letters and in bold type.
Make sure that you understand and follow all safety messages
presented in this manual.
1.2.2 Special Notices and Informational Notes
Special notices and informational notes in this manual appear different
from the main flow of text. They appear in boxes and a note label
identifies them. The label text appears in uppercase letters and in bold
type.
NOTICE Highlights information necessary to prevent damage to the
detector or invalid test results.
TIP Highlights information of general interest or helpful information that
can make a task easier or optimize the performance of the detector.

1•Using this Manual
Page 12 Vanquish Diode Array Detectors (VH-D10) Operating Manual
1.2.3 Typographical Conventions
These typographical conventions apply to the descriptions in this
manual:
Data Input and Output
•The following appears in bold type:
♦Input that you enter by the keyboard or that you select with the
mouse
♦Buttons that you click on the screen
♦Commands that you enter by the keyboard
♦Names of, for example, dialog boxes, properties, and parameters
•For brevity, long expressions and paths appear in the condensed
form, for example: Click Start > All Programs > Thermo
Chromeleon 7 > Services Manager > Start Instrument Controller.
References and Messages
•References to additional documentation appear italicized.
•Messages that appear on the screen are identified by quotation
marks.
Viewpoint
If not otherwise stated, the expressions left and right in this manual
always refer to the viewpoint of a person that is facing the detector from
the front.
Particularly Important Words
Particularly important words in the main flow of text appear italicized.
Electronic Manual Version (PDF)
The electronic version (PDF) of the manual contains numerous links that
you can click to go to other locations within the manual. These include:
•Table of contents entries
•Index entries
•Cross-references (in blue text), for example, to sections and figures

1•Using this Manual
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 13
1.3 Reference Documentation
In addition to this operating manual, other documentation is available
for reference.
Hardware Documentation
Additional hardware documentation includes the following:
•Operating manuals for the other modules of the Vanquish system
A printed version of the manual is shipped with the device.
•Vanquish System Operating Manual
A printed version of the manual is shipped with the Vanquish system
base and solvent rack.
•Instrument Installation Qualification Operating Instructions
TIP Electronic versions of these manuals are available as PDF (Portable
Document Format) files. To open and read the PDF files, Adobe™
Reader™ or Adobe™ Acrobat™ is required.
Software Documentation
Additional software documentation includes the following:
•Chromeleon™ 7 Help and documents
The Chromeleon 7 Help provides extensive information and
comprehensive reference material for all aspects of the software.
For basic information about device installation and configuration,
refer to the Installation Guide; for specific information about a
certain device, refer to the Instrument Configuration Manager Help.
In Chromeleon 7, devices are called modules.
For information about the main elements of the user interface and
step-by-step guidance through the most important workflows, refer
to the Quick Start Guide.
For a concise overview of the most important workflows, refer to
the Reference Card.
•Chromeleon™ 6.8 Help
The Chromeleon 6.8 Help provides extensive information for all
aspects of the software, including device installation and
configuration.

1•Using this Manual
Page 14 Vanquish Diode Array Detectors (VH-D10) Operating Manual
TIP The Chromeleon Help and documents are included in the software
shipment.
Third-Party Documentation
Refer also to the user documentation provided by the manufacturers of
third-party components and materials, for example, Safety Data Sheets
(SDSs).

2•Safety
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 15
2Safety
This chapter provides general and specific safety information and informs
about the intended use of the detector.

2•Safety
Page 16 Vanquish Diode Array Detectors (VH-D10) Operating Manual
2.1 Safety Symbols and Signal Words
2.1.1 Safety Symbols and Signal Words in This Manual
This manual contains safety messages to prevent injury of the persons
using the detector. The safety symbols and signal words in this manual
include the following:
Always be aware of the safety information. Do not proceed until you
have fully understood the information and consider the consequences of
what you are doing.
CAUTION Indicates a hazardous situation that, if not avoided, could
result in minor or moderate injury.
WARNING Indicates a hazardous situation that, if not avoided, could
result in serious injury.

2•Safety
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 17
2.1.2 Observing this Manual
Observe the following:
•Before installing or operating the detector, read this manual
carefully to be familiar with the detector and this manual. The
manual contains important information with regard to user safety as
well as use and care of the detector.
•Always keep the manual near the detector for quick reference.
•Save this manual and pass it on to any subsequent user.
Read, understand, and comply with all safety messages and
precautionary statements presented in this manual.
2.1.3 Safety Symbols on the Detector
The table lists the safety symbols that appear on the detector or on
labels affixed to the detector. Follow the safety notices in this manual to
prevent the risk of operator injury or damage to the detector.
Symbol Description
Indicates a potential hazard. Refer to this manual to avoid the risk of
personal injury and/or to prevent damage to the device.
—
Ο
Power supply is on
Power supply is off
Indicates alternating current.
Indicates that the surface becomes hot during operation. Do not
touch these surfaces while they are heated up.
Indicates that the UV radiation produced by the deuterium lamp in
the device may be harmful to eyes and skin. Do not look directly into
the light produced by the deuterium lamp. Never operate the device
without the lamp cover installed and never operate the lamp outside
the device.
˜

2•Safety
Page 18 Vanquish Diode Array Detectors (VH-D10) Operating Manual
2.1.4 Rating Plate
The rating plate is present on the detector near the electrical
connections. The rating plate indicates the serial number, part number,
module name, revision number (if any), line and fuse rating, and the
manufacturer's address.
TIP An additional type label on the leak tray of the detector indicates the
module name, serial number, part number, and revision number (if any).
To facilitate device identification have the information from this label
available when communicating with Thermo Fisher Scientific.

2•Safety
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 19
2.2 Intended Use
The detector is intended to be part of the Vanquish system. The
intended use of the Vanquish system is to analyze mixtures of
compounds in sample solutions. The detector is for use by qualified
personnel and in laboratory environment only.
The detector and Vanquish system are intended for laboratory research
use only. They are not intended for use in diagnostic procedures.
Laboratory Practice
Thermo Fisher Scientific recommends that the laboratory in which the
Vanquish system is used follow best practices for LC analyses. This
includes among others:
•Using appropriate standards
•Regularly running calibration
•Establishing shelf life limits and following them for all consumables
used with the system
•Running the system according to the laboratory's verified and
validated 'lab developed test' protocol

2•Safety
Page 20 Vanquish Diode Array Detectors (VH-D10) Operating Manual
2.3 Safety Precautions
2.3.1 General Safety Information
All users must observe the general safety information presented in this
section and all specific safety messages and precautionary statements
elsewhere in this manual during all phases of installation, operation,
troubleshooting, maintenance, shutdown, and transport of the detector.
If the detector is used in a manner not specified by Thermo Fisher
Scientific, the protection provided by the detector could be impaired.
Observe the following:
•Operate the detector only within its technical specifications.
•Use only the replacement parts and additional components, options,
and peripherals specifically authorized and qualified for the detector
by Thermo Fisher Scientific.
•Perform only the procedures that are described in this operating
manual and in supporting documents for the detector.
Follow all instructions step by step and use the tools recommended
for the procedure.
•Open the enclosure of the detector and other components only if
specifically instructed to do so in this manual.
•Thermo Fisher Scientific cannot be held liable for any damage,
material or otherwise, resulting from inappropriate or improper use
of the detector. If there is any question regarding appropriate usage,
contact Thermo Fisher Scientific before proceeding.
Safety Standard
This device is a Safety Class I instrument (provided with terminal for
protective grounding). The device has been manufactured and tested
according to international safety standards.