Tissue-Tek Glas g2 User manual

Tissue-Tek®Glas™g2
Automated Glass Coverslipper
Ensure
Quick Reference Guide
The Tissue-Tek®Glas™g2 automated coverslipper is
designed to coverslip biological specimens that are
mounted on microscope slides using cover glasses. The
instrument, as part of the histopathology process, is
intended to facilitate the in vitro examination of human
and animal tissue for morphology changes by a
pathologist.
This Quick Reference Guide contains information about
day-to-day use, maintenance and cleaning. For more
detailed information we refer to the most recent
Operating Manual of this instrument.
All users should be trained for the use of the Sakura
instruments. The Sakura User Training is a training to
ensure that new and existing users of a Sakura
instrument have the knowledge and skills to operate and
maintain the instrument in a proper way.
Daily operations
To operate the instrument switch on the “POWER”
button located near the control panel.
By turning on the power there will be a pre-operational
system check.
Ensure that during this check the instrument contains
sufficient coverslip glasses, mounting medium and
solvent. When any of these do not reach their specified
level, add more until this requirement is met.
Check if any slides remain on the transfer area, and
remove them before proceeding.
When placing the mounting medium tilt the mounting
medium tube toward the front and lift it slightly. In order
to prevent air bubbles, insert the tube gently into the
bottle.
Version : 2019-1

Check the loading area and press “ENTER” when this is
empty to start the home positioning procedure. When
this has been completed the display switches to standby
mode.
When the coverslipper is connected to a Prisma series
stainer, the basket is stored in a linked end station and
coverslipped automatically after the chosen staining
protocol is done.
In order to manually place a basket in the coverslipper,
open the loading station access door and pull out the
loading station. Put in the basket with the “Up-side”
facing towards the right, slide the loading station back in
and close the door. The process of coverslipping will
start automatically.
Ensure in both cases the slides are placed properly in
the basket according to the image shown.
Note: Fill the loading station with solvent to avoid slides
from drying out.
The receiving racks located on the left side of the
instrument are used to store the coverslipped slides. A
rack that has been placed slightly more towards the
output door indicates that the rack contains coverslipped
slides. Careful when removing these slides, when the
rack is slightly tilted slides may fall out.
The carousel on the right contains the empty slide
baskets and should be removed periodically.
Maintenance introduction
First review the Operating Manual Tissue-Tek®Glas™
g2 for the complete cleaning instructions.
When used under normal conditions the dispensing
nozzle is placed just above the solvent level within the
anti-drying bottle in order to keep the nozzle within the
solvent vapours. This situation will keep the mounting
medium from solidifying at the end of the line, keeping
the dispensed quantity at the set amount.
Only when the instrument is not used for an extended
period oftime (more thanone week) the lever in the back
of the instrument is pressed, this action will bring down
the dispensing nozzle into the solvent. This keeps the
mounting medium within the dispensing line from
solidifying and causing obstructions over time.
If operations are resumed, press the “PRIME”key to
discharge mounting medium that has been mixed with
solvent.

Maintenance
Daily:
Station cleaning
Ensure the system to be free of dust. Remove
foreign matters and glass powder with a brush.
Use a cloth moistened with solvent matching to
the mounting medium to remove any solidified
medium attached to the stations.
Cover glass arm
Remove oil residue on the suction pad using a
cloth moistened with 100% alcohol.
Dispensing nozzle
Wipe the dispensing nozzle by using a cloth
moistened with solvent matching the used
mounting medium.
Waste container
Remove the waste container and replace it with
a clean one. Check if the spatula part is still
intact. Soak the used waste container for a day
in solvent matching the mounting medium.
Weekly:
Loading station
Replace the used solvent in the loading station
and remove at the sametime all foreign matters
to minimise contamination.
Nozzle anti-drying bottle
Refresh the solvent in the anti-drying bottle
every week or when it becomes cloudy to
ensure its effectiveness. This bottle is located at
the end of the dispensing line. Ensure to refill
just below the lid.
Receiving racks
Soak the racks for a full day in the appropriate
solvent and let them dry completely before
using them again.
Slide arm and detector
Clean the slide arm and slide detection sensor
using a cloth moistened with 100% alcohol.
Every two months:
Pump anti-drying bottle
As this bottle is stuck in its place, remove the lid
and take out the solvent with a pipette. Refresh
by filling the bottle with solvent just below the lid
as shown earlier at the other bottle.
Yearly:
Preventative maintenance
Preventative maintenance is performed by a
Sakura representative or a qualified service
engineer.

Maintenance task
Frequency
Station cleaning
Daily
Cover glass arm
Daily
Dispensing nozzle
Daily
Waste container
Daily
Loading station
Weekly
Nozzle anti-drying bottle
Weekly (or when cloudy)
Receiving racks
Weekly
Slide arm and detector
Weekly
Pump anti drying bottle
Every two months
Preventative maintenance
Yearly
Always use a solvent matching the mounting medium