Tissue-Tek TEC 5100 User manual

Operating
Manual
Tissue Embedding Console System
Tissue-Tek® TEC™5
MODEL NUMBERS
5100 (115 VAC, America)
5229 (230 VAC, Europe)
5232 (220-240 VAC, Asia)
5235 (115 VAC, Asia)
5238 (100 VAC, Asia)

IMPORTANT NOTICE
If this equipment is used in a manner not
specified by the manufacturer, the protection
provided by the equipment may be impaired.
© 2011 Sakura Finetek USA, Inc.
All Rights Reserved
Printed in U.S.A.
Manufactured for:
Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 135-0007, Japan
Sakura Finetek Europe B.V., 2804 AV Alphen aan den Rijn, NL
0001338-02 Rev.C

Revised 07/12/11 iii
Section Page
1 INTRODUCTION
General Description .......................................................................1.1
Features ...................................................................................1.1
Operation: Warm Up and Ready Times ....................................................1.2
Specifications .............................................................................1.3
2 UNPACKING, SYSTEM DESCRIPTION
Unpacking ................................................................................2.1
Carton Contents ...........................................................................2.1
System Description
Embedding Module (Front Panel) .......................................................2.2
Embedding Module (Back Panel) .......................................................2.3
Accessories .............................................................................2.4
Embedding Module (Control Panel) .....................................................2.5
Cryo Module (Front Panel)..............................................................2.7
Cryo Module (Back Panel) ..............................................................2.7
Audible Alarms .........................................................................2.7
3 SET UP
Installation ................................................................................3.1
Attach Magnifying Lens Assembly ........................................................3.2
Verify Proper Installation/System Functioning/Power On...................................3.3
Access Set Up Mode ......................................................................3.4
Set Day and Time .........................................................................3.4
Set Cryo Module Mode of Operation ......................................................3.4
Input Cryo Offset Valve ...................................................................3.4
Change or View Set Temperatures .........................................................3.5
Add Paraffin...............................................................................3.6
Place Base Molds into Chamber ...........................................................3.6
What Happens When Power Is Turned Off.................................................3.7
When To Set Power Switches On Front Panel To Off ......................................3.7
4 AUTOMATIC TIMER (OPTIONAL USE)
EMBEDDING MODULE
ON and OFF Times for Selected Days of the Week.........................................4.1
Setting the Automatic Timer ...............................................................4.1
Checking the Status of the Automatic Timer (What Time is Set?)...........................4.2
5 EMBEDDING PROCEDURE
Embedding Procedure (example) ..........................................................5.1
TABLE OF CONTENTS

iv Revised 07/12/11
Section Page
6 TROUBLESHOOTING
Error Condition, Cause, Solution ..........................................................6.1
7 CARE OF INSTRUMENT AND MINOR REPAIRS
Instrument Care ...........................................................................7.1
Routine Maintenance Chart (summary) ....................................................7.1
Daily Maintenance ........................................................................7.2
Disinfection ...............................................................................7.2
Periodic Maintenance (As Required).......................................................7.3
Minor Repair ..............................................................................7.6
8 SERVICE AND REPLACEMENT PARTS
Service ....................................................................................8.1
Replacement Parts .........................................................................8.3
Accessories................................................................................8.3
Other Available Equipment and Accessories ...............................................8.4
TABLE OF CONTENTS

Revised 07/12/11 1.1
INTRODUCTION
GENERAL DESCRIPTION
The Tissue-Tek®TEC™ 5 Tissue
Embedding Console System is used to
embed processed tissue in paraffin, form-
ing paraffin blocks ready for sectioning.
The System consists of:
QEmbedding Module
QCryo Module
QTampers (1 large and 1 small)
QTransfer Tray
QPlastic Scraper
QFoot Pedal (sold separately, see Section 8)
QMagnifying Lens (sold separately, see Section 8)
QElectric Forceps (sold separately, see Section 8)
QBase Molds (sold separately, see Section 8)
QTissue Cassettes (sold separately, see Section 8)
FEATURES
QA large insulated work space (allows you to
manipulate more samples comfortably and effi-
ciently)
QIndividual temperature control in six areas (hot
plate, paraffin chamber, left and right chambers,
Cryo Module, optional electric forceps)
QUp-front keypad and LCD display (for easy
access)
QTwo 3-hole forceps holders located on each side
of the paraffin dispenser (for right or left hand
operations)
QMenu-driven software simplifies setup of the
Automatic Timer (you specify the time you
require paraffin to be ready. The instrument cal-
culates the time it should automatically turn the
heaters on)
QEmbedding and Cryo Modules work areas of
the same height (so modules can be placed side
by side, in any order to facilitate your choice of
“right-to-left” or “left-to-right” work flow)
QLarge capacity (4L) paraffin reservoir with
optimized paraffin dispensing rates (do more
work, faster)
QLarger cold spot (accepts larger base molds)
QHinged, thermal chamber doors move com-
pletely out of the way (allows full access to both
thermal chambers)
QOptional magnifying lens (enhances visibility
while positioning tissue specimen)
QOptional foot pedal (for hand-free operation)
QOptional electric forceps (always at the right
temperature to allow for more efficient workflow)
Section 1

OPERATION:
WARM UP and READY TIMES
Embedding Module
When the Embedding Module is turned on, either
manually or automatically, 4 hours* is required for
paraffin to liquefy and reach set temperature.
The Embedding Module stores the instrument set-
tings in memory whenever power is turned off or is
lost. When power is restored or turned on, a Power
Outage message will appear on the display if power
was turned off or lost when the instrument status was
<READY>, <WARMING UP>, or <CRYO>. A
Power Outage message will not be given if the
instrument status was <STANDBY>.
Manual Mode: Set power switch on front panels
to ON; press AUTO/MAN key to MAN (green indi-
cator illuminates). Five areas begin to heat when the
HEAT key is pressed ON. All areas, except the
paraffin chamber and the left and right chambers,
reach set temperature within 60 minutes. The paraf-
fin and the left and right chambers reach set temper-
ature in 4 hours.* Using paraffin already in the
molten state will significantly reduce the warming
up time. The instrument status will be <WARMING
UP>. When all heated areas reach set temperature,
the instrument status will change to <READY>.
The heat remains ON until you manually press the
HEAT key again.
Automatic Timer Mode: The Embedding Module
can be preset to be ready to use at your specified
time. The instrument calculates an automatic “turn
on” time from your preset ready time. When the
instrument automatically turns ON, only the paraffin
chamber begins to heat. The remaining four areas
begin to heat 60 minutes prior to the preset ready
time. During that time, the instrument status will be
<WARMING UP>. When all set temperatures are
reached, the instrument status will change to
<READY>. The module automatically turns OFF at
your preset “Shut down” time or if you select the
MANUAL mode and press HEAT.
*May be longer depending on current source and environmental
factors.
Cryo Module
The Cryo Module is ready to use (reaches set
temperature) 15 minutes after the compressor is
turned ON. It can also be turned on automatically
using the automatic timer mode.
Temperature can be set down to -10°C in 1°C incre-
ments. The Cryo Module must be connected to the
Embedding Module to operate.
NOTE: Make sure the Cryo Module is connected to
the Embedding Module and that the power is on. If
the Cryo Module is not powered up or not connected
to the Embedding Module, and if the CRYO key on
the Embedding Module key is pressed ON or the
Automatic Timer software tries to turn the Cryo
Module ON, the following error message will be
given within 15 seconds:
To silence the alarm press any key.
To operate the Cryo Module if this situation occurs,
first turn the Embedding Module power switch to
OFF, verify that the Cryo Module is connected to the
Embedding Module, and turn the Cryo Module
power switch to ON. Then turn the Embedding
Module ON again.
NOTE: The Cryo Module’s power indicator will
stay on when the Cryo Module is turned off using
the Cryo key on the Embedding Module.
Ensure your safety! Comply with all
WARNING and CAUTION statements found
in this manual.
1.2 Revised 07/12/11
INTRODUCTION
<CRYO ERROR> CRYO POWER IS OFF OR
COOLING FAN ERROR

Revised 07/12/11 1.3
SPECIFICATIONS
EMBEDDING CRYO
MODULE MODULE
Ambient Operating 10° – 40° C 10° – 40° C
Temperature and
Relative Humidity Range 30% – 85% RH, 30% – 85% RH
(noncondensing) (noncondensing)
Dimensions/Weight Width 57.5 cm (22.6 in) Width 33.0 cm (13.0 in)
Depth 64.2 cm (25.3 in) Depth 61.7 cm (24.3 in)
Height 37.7 cm (14.8 in) Height 37.7 cm (14.8 in)
Weight approx. 26 kgs (57 lbs) Weight approx. 22 kgs (48 lbs)
Power Required Model No. 5238: 100 VAC ±10% or less,
100 VAC ±10% or less, @ 2.0 amps 50/60 Hz
@ 9.0 amps 50/60 Hz
Model Nos. 5100, 5235: 115 VAC ±10% or less,
115 VAC ±10% or less, @ 2.0 amps 60 Hz
@ 9.0 amps 60 Hz
Model Nos. 5229, 5232: 220 – 240 VAC ±10% or
220 – 240 VAC ±10% or less, @ 2.0 amps 50 Hz
less, @ 9.0 amps 50 Hz
Chamber Capacity Paraffin Chamber:
approx. 4 liters (no chambers)
Left and Right Chambers:
approx. 1.5 liters
Temperature Range Paraffin Chamber, Right and Cooling Plate:
Available Left Chambers, Hot Plate: –10°C to 0°C
50°C – 75°C
Electric Forceps(optional):1 to 5
Factory Set Temperature Paraffin Chamber, Left and Cooling Plate:
Right Chambers, Hot Plate: – 5°C
62°C
Cold Spot: approx. 15°C at
25°C room temp. and
40% R.H.
Electric Forceps(optional): 3
Circuit Breaker/ON-OFF 15 amps
Switch
Memory Backup Long-life battery
Safety Standards Tested and listed by Intertek Testing Services (ITS)
Complies with: UL 3101-1, 1st ed., IEC 1010-1 and
CAN/CSA-C22.2 No. 1010.1-92

Revised 07/12/11 2.1
UNPACKING,
SYSTEM DESCRIPTION
UNPACKING
The Tissue-Tek®TEC™ 5 Tissue Embedding
Console System arrives in two cartons tied together.
Inspect each carton for shipping damage.
Unpack each carton.
CARTON CONTENTS
Embedding Module
Cryo Module
Cryo Module Power Cord
Transfer Tray
Tampers (1 large and 1 small)
Plastic Scraper
Connecting Cable to Embedding Module
Embedding Module Power Cord
Operating Manual (not shown – instructions for
both Embedding and Cryo Modules)
QUse extreme care when unpacking each module.
Each module contains sensitive electronic parts.
Check each item for shipping damage.
QIf there is visible shipping damage, immediately
file a complaint with the carrier. Then, notify your
nearest Tissue-Tek instrument distributor.
Missing items?…Dial 1-800-725-8723 (U.S.
Customers, only). If located outside the U.S.,
contact your nearest Tissue-Tek instrument
distributor.
Section 2





 

2.2 Revised 07/12/11
UNPACKING,
SYSTEM DESCRIPTION
SYSTEM DESCRIPTION
Embedding Module
(FRONT PANEL)
Paraffin Chamber – holds up to four liters of
molten paraffin; fine mesh filter at bottom of
chamber catches debris; can be set to any tem-
perature at or between 50°–75°C (122°–167°F);
factory set at 62°C (143.6°F).
Power Switch – turns the power to the module
ON or OFF; circuit breaker.
Control Panel – consists of an LCD display and
keypads (each component discussed in detail
later in this section).
(Optional) Electric Forceps – (Connector
shown only) connects underneath the control
panel. Adjustable to 5 different temperature lev-
els.
Forceps Warmer Holders – hold forceps; can
be set to any temperature at or between
50°–75°C (122°–167°C) using the Hot Plate
temperature settings; factory set at 62°C (149°F).
Paraffin Dispenser – molten paraffin is dis-
pensed here; press fingerplate to dispense paraf-
fin or use the optional foot pedal; turn knob
“clockwise” to decrease flow or “counterclock-
wise” to increase flow. Optional foot pedal (not
shown) can be used instead of the fingerplate to
dispense paraffin.
Work Light – increases visibility in hot plate
area making tissue positioning easier.
(Optional) Magnifying Lens Assembly –
increases visibility while positioning tissue.














Left and Right Chambers – can be set to any
temperature at or between 50° – 75°C
(122°–167°F); each chamber can be used for one
of the following at any one time (preference
depends on your chosen work flow direction):
Qwarming area for various sized base molds (close
lid to keep heat in and dust out)
OR
Qparaffin bath to keep processed tissue specimens at
the appropriate temperature (close lid to keep heat
in, and dust out, when embedding process is fin-
ished or temporarily stopped).
Transfer Tray – may be used to transfer
processed specimens to the Embedding Module
or may be half-filled with paraffin and then used
as a paraffin bath.
Waste Drawers – catch molten paraffin that drains
through two holes in grooves around hot plate
area.
Cold Spot – cools liquid paraffin at the bottom
of a base mold so tissue can be correctly posi-
tioned.
Hot Plate – ensures that paraffin does not solidi-
fy during embedding process; can be set to any
temperature at or between 50°– 75°C
(122°–167°F); factory set at 62°C (143°F). This
temperature setting is also used for the Forceps
Warmer Holders.
(Optional) Foot Pedal – for hands-free paraffin
dispensing.
Display Adjustment Wheel – controls the con-
trast for the display.
Embedding Module
(BACK PANEL)
Cryo Module Connector – accepts the plug to
the Cryo Module. The Cryo Module operation is
controlled by the Embedding Module.
Power Cord Connector – accepts the plug for
connecting the power cord to the module.
Revised 07/12/11 2.3




Accessories
(found inside paraffin chamber; refer to photo on
page 2.1)
Embedding Module Power Cord – carries electri-
cal current to Embedding Module
Tampers (1 large and 1 small) – used (as neces-
sary) to press tissue firmly against the bottom of the
base mold
Plastic Scraper – used to remove frost from cooling
plate of Cryo Module
2.4 Revised 07/12/11
UNPACKING,
SYSTEM DESCRIPTION

Revised 07/12/11 2.5
Embedding Module
(CONTROL PANEL)
 LCD Display – consist of two lines of up
to 40 characters per line.
Displays (depending on the mode):
(a) current time of day
(b) On or Off times
(c) current temperatures
(d) actual preset temperatures
(e) actual operating mode
(f) system status information and error condi-
tions
 Left and right arrow keys– move the display
cursor left and right. Rapid scrolling is enabled
by holding these keys for more than 2 seconds.
 AUTO/MAN key – selects between automatic
and manual operation. The corresponding LED
will be lit to indicate the current mode of opera-
tion. Selecting the Manual Mode of operation
does not permanently cancel the automatic timer
On/Off times.
 LIGHT key– turns Work Light ON or OFF.
 CRYO key – turns the Cryo Module ON or OFF.
The independent operation of the CRYO Module
can be restricted if the Automatic Timer has been
programmed to also control the ON and OFF
times of the Cryo Module. Under this condition,
the CRYO key will not operate when the system
operating mode is in AUTO (AUTO LED indica-
tor is ON). See Section 3, SET UP to change the
setting. NOTE: Power to the Cryo Module
must be turned on before the Embedding Module
is turned ON.








2.6 Revised 07/12/11
UNPACKING,
SYSTEM DESCRIPTION
 HEAT Key – turns the heating components of
the Embedding Module ON or OFF.
 SET key – allows operator to enter the SETUP
mode (See Section 3, Set Up).
 EXIT key – allows to return to the previous
menu item without saving the changes made in
the SETUP mode.
 + and – keys – increase or decrease numerical
values and add or delete days when in the
SETUP mode. Holding these keys for more than
2 seconds enables rapid scrolling.
 ENTER key – when pressed, stores data into
memory in the SETUP mode.
 Display Adjustment Wheel – controls the con-
trast for the display.

Revised 07/12/11 2.7






Revised 07/12/11 3.1
SET UP
INSTALLATION
CAUTION: Do not place the Tissue-Tek®TEC™ 5
Embedding System where it would be subjected
to direct sunlight and/or direct heat, an open
flame source, excessive humidity or air current,
excessive particulate matter, or electrical noise
(such as from refrigerators, centrifuges, ultra-
sonic cleaners and microwave ovens).
1. Select a level surface near one properly ground-
ed electrical outlet in a well-ventilated area of
minimum humidity.
CAUTION: Make sure the electrical outlet
supplies the proper voltage (within ± 10%).
Refer to the rating label on the Embedding
Module.
2. Place the Embedding Module next to the Cryo
Module. It is not necessary to leave any space
between the two modules.
NOTE: The working area of each module is of
the same height. This allows you to place the
modules next to each other, in any order, to
accommodate your choice of “right-to-left” or
“left-to-right” work flow.
3. Check to make sure the power switches on the
front panel of the Embedding Module and the
Cryo Module are set to OFF (O).
4. Connect the Cryo Module to the Embedding
Module using the provided cable. The end of
this cable having the right-angle connector is
plugged into the Cryo Module. The end of this
cable having the straight connector is plugged
into the Embedding Module.
5. Connect the Cryo Module and Embedding
Module power cords into properly grounded out-
lets. The power cord with the right angle con-
nector plugs into the Cryo Module.
6. Connect the Foot Pedal (sold seperately, refer to
section 8) to the back of the Embedding Module.
7. Move the modules into place on the designated
work space.
CAUTION: For cooling purposes, allow at
least 76 mm (3 in) of space between the wall
(or other surface) and the back panel of each
module.
Section 3

ATTACH MAGNIFYING LENS
ASSEMBLY
(sold separately, refer to Section 8)
1. Remove the screw from the top of the
paraffin dispenser.
2. Mount assembly in the threaded hole of the
paraffin dispenser. The end of the flexible attach-
ment screws directly into the hole. Tighten.
3. Adjust magnifying lens to preferred focal range
(pull lens forward and down).
CONNECT ELECTRIC FORCEPS
(sold separately, refer to section 8)
1. Connect the wire underneath the Embedding
Module control panel.
3.2 Revised 07/12/11
SET UP

VERIFY PROPER INSTALLATION
AND SYSTEM FUNCTION
1. Set the power switch on the front panel of the
Cryo Module to ON (I).
2. Set the power switch on the front panel of the
Embedding Module to ON(I).
The MAN indicator is illuminated.
The following appears on the LCD display:
(The time and day may be different)
If the LCD display appears dark (no characters
visible), rotate the display adjustment wheel fully
in both directions until the display characters are
easily read.
3. Press HEAT key (on Embedding Module control
panel). The following appears on the LCD dis-
play:
4. Press CRYO key (on Embedding Module control
panel).
The “CRYO IS OFF” message will disappear
from the display and the compressor will start.
If System functions properly:
Qpress CRYO key to turn off compressor (the mes-
sage CRYO IS OFF is back on the display);
Qcontinue with “Set Day and Time ” procedure
(next page).
NOTE: Paraffin will not be dispensed and an audi-
ble alarm will sound if the fingerplate or the optional
foot pedal is pressed unless <READY> is displayed
on the top left end corner.
If System does not function properly:
Dial 1-800-725-8723 (U.S. Customers, only). If
located outside the U.S., contact your nearest Tissue-
Tek instrument distributor.
Revised 07/12/11 3.3
<WARMING UP> CRYO IS OFF 6:30PM WED
PLEASE WAIT.
<STAND BY> 6:30PM WED

ACCESS SET UP MODE
Access the SET UP mode by pressing the SET key.
The first two menu items are displayed.
The other five menu items are:
3. TIMER 4. CLOCK
5. TIME FORMAT 6. ERROR LIST
7. CRYO OFFSET
Pressing the ENTER key will allow access to the
item number highlighted by the cursor.
SET DAY AND TIME
QClock settings are not lost when a power failure
occurs or when the Embedding Module power
switch (on the front panel) is set to OFF. The
instrument memory is backed-up by a battery. At
installation and periodically as required, it may be
necessary to set the clock.
To select a 12-hour or 24-hour time format, use the
arrow keys to move the cursor to item “5. TIME
FORMAT” and press ENTER. Then, use the arrow
keys to select the desired format and press ENTER.
When ready to set the actual day and time, use the
arrow keys to move the cursor to item “4. CLOCK”
and press ENTER. Use the Arrow keys to highlight
the actual day of the week and press ENTER. A
prompt will then appear to input the current time.
Use the Arrow keys to move the cursor between
“Hour” and “Minutes”. Use the + and - keys to set
the actual values. When finished, press ENTER to
save the values into memory. If you do not want to
save the changes, press the EXIT key instead.
SET CRYO MODULE MODE OF
OPERATION
The Cryo Module can be operated independently of
the Embedding Module when the MAN/AUTO keys
is set to MANUAL (MAN LED is illuminated). The
CRYO key can then be used to turn the Cryo Module
ON or OFF.
NOTE: The Cryo Module power switch must be
turned ON first. The Embedding Module power
switch must then be ON to operate the Cryo module.
When AUTOMATIC operation is selected (AUTO
LED is illuminated) control of the Cryo Module ON
and OFF times can be synchronized with the ON and
OFF times of the Embedding Module or can be inde-
pendent. To synchronize the operation of the Cryo
Module with the Embedding Module, enter the
SETUP mode by pressing, the SET key and high-
light “2. CRYO MODULE”. Press ENTER and
select item “1. TIMER ON” using the Arrow keys
and pressing ENTER. If independent operation of
the Cryo Module is preferred, select “2. TIMER
OFF”. If the Timer OFF option is selected, the
CRYO key must be pressed to turn the Cryo Module
on.
INPUT CRYO OFFSET VALUE
Since the Embedding Module computer controls the
operation of the Cryo Module, an offset value must
be entered to ensure temperature accuracy.
Write down the offset value found on the Cryo
Offset Label located at the back of the Cryo Module.
The range is -4.9 to +4.9.
Enter the SETUP mode by pressing the SET key and
highlight “7. CRYO OFFSET”. Press ENTER and
use the + and - keys to set the actual value. When
finished, press ENTER to save the value into memo-
ry. If you do not want to save the changes, press the
EXIT key instead.
3.4 Revised 07/12/11
SET UP
SETUP MODE> SELECT MENU (1-7)
1. TEMPERATURE 2. CRYO MODULE