Agilent Technologies Genomic DNA ScreenTape System User manual

Agilent Technologies
Agilent Genomic DNA ScreenTape
System Quick Guide
System Components
The Agilent 2200 TapeStation system is an automated platform for simpler, faster and more reliable
electrophoresis.
It is made up of three elements:
•2200 TapeStation System (G2964AA) or 2200 TapeStation Nucleic Acid System (G2965AA)
•Genomic DNA ScreenTape (5067- 5365) with Genomic DNA Reagents (Ladder and Sample Buffer)
(5067- 5366)
•Agilent 2200 TapeStation Software
Kit
The Genomic DNA ScreenTape assay is designed for analyzing genomic DNA samples in the size range
from 200 bp to >60000 bp.
Specifications
Analytical Specification Genomic DNA ScreenTape Assay and Reagents
Sizing Range 200 bp to > 60000 bp
Sensitivity 0.5 ng/µL
Sizing Precision1
1Determined using the Genomic DNA Ladder as sample
200 – 15000 bp: 15 % CV
Sizing Accuracy1200 – 15000 bp: ±15 %
Quantitative Precision2
2Average result from various genomic DNA sample types
15 % CV
Quantitative Accuracy2±20 %
Linear Concentration Range 10 – 100 ng/µL
DIN functional range 5 – 300 ng/µL
Physical Specification
Analysis Time 16 samples: < 25 min, 96 samples: < 150 min
Samples per consumable 16
Sample Volume Required 1 µL
Shelf Life 4 months
Box/Kit size 112 samples/box

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Agilent Genomic DNA ScreenTape System Quick Guide
Storage Conditions
•Reagent vials: 2 – 8 °C
•The ScreenTape device: 2 – 8 °C (if you run less than 16 lanes, store used ScreenTape device
upright at 2 – 8 °C for a maximum of 2 weeks.)
•Never freeze the ScreenTape device - any ScreenTape device which is accidentally frozen should be
discarded.
Kit Components
Additional Consumables Required for the 2200 TapeStation Instrument
•Loading tips (5067- 5152 or 5067- 5153)
•Optical Tube 8x Strip (401428) and Optical Cap 8x Strip (401425) or 96- well Sample Plates
(5067- 5150) and 96- well Plate Foil Seal (5067- 5154).
•Vortex mixer (See note below)
Additional Material Required (Not Supplied)
•Volumetric pipette
•Micro- centrifuge
Safety Information
Part Number Name Color Amount
5067-5365 Genomic DNA ScreenTape 7 ScreenTape devices
5067-5366 Genomic DNA Reagents
•Genomic DNA Ladder
•Genomic DNA Sample Buffer
2 vials
25 µL
1350 µL
NOTE
TapeStation instruments are supplied with an optional IKA MS3 vortexer which includes a
96-well plate adaptor suitable for both 96-well PCR plates and 8-way strips.
WARNING
Toxic agents
The handling of solvents, samples and reagents can hold health and safety risks.
➔When using/handling the ScreenTape device and working with these substances observe
appropriate safety procedures (for example by wearing goggles, safety gloves and protective
clothing).
➔Always follow good laboratory practices and adhere to the guidelines established in your
laboratory.
➔Refer to product material safety datasheets for further information.
➔The volume of substances should be reduced to the minimum required for the analysis.
CAUTION
Damage to the 2200 TapeStation instrument
➔Use only the recommended consumables and reagents with the 2200 TapeStation system.

3
Agilent Genomic DNA ScreenTape System Quick Guide
General Information on Working with Genomic DNA
Essential Measurement Practices
NOTE
•For best results ensure that all reagents are allowed to equilibrate to room temperature
for 30 min prior to use.
•When pipetting Sample Buffer, ensure that excess buffer droplets are removed from the
tip before transfer to the sample tubes. Care must be taken due to the viscosity of
Sample Buffer.
•When pipetting small volumes ensure that no sample remains within the tip.
•When adding sample buffer to sample or ladder, please ensure that they are mixed
correctly. To achieve this, gently mix several times with additional pipetting, then cap the
tubes, vortex mix using IKA vortexer and adaptor at 2000 rpm for 1 min.
•Briefly centrifuge to collect the contents at the base of the tubes.
•Improper mixing can lead to quantification errors.
Environmental
conditions
•Optimal operating temperature: 20 °C (68 F)
•Ambient operating temperature: 15 – 30 °C (59 – 86 F)
Steps before use on
the TapeStation
•Equilibrate each vial to room temperature for 30 min.
•Gently vortex mix each vial and briefly spin.
•'Flick' ScreenTape device to eliminate bubbles in the separation channel, which could interfere
with sample loading.
•Do not shake or over mix ladder vial, this could result in degradation of the Genomic DNA
ladder.
Steps during sample
preparation
•Keep reagents at room temperature during sample preparation.
Storage after use on
the TapeStation
•Store all reagent vials and ScreenTape devices at 2 – 8 °C
•Never store reagent vials or ScreenTape devices at room temperature or below 0 °C.
•If you run less than 16 lanes, store used ScreenTape device upright at 2 – 8 °C for maximum of 2
weeks.
Pipette carefully •Always pipette reagents against the side of the sample tube.
•If using a standard pipette ensure that no residual material is left on the outside of the tip.
Mix properly after
each pipetting step
•Mix = Vortex the PCR tubes or 96-well plate using Agilent approved IKA vortexer and adaptor at
2000 rpm for 1 min.
•Spin = Move the samples to the bottom of the tubes/wells by pulsing in a centrifuge.

Agilent Genomic DNA ScreenTape System Quick Guide
*G2964-90040*
*G2964-90040*
G2964-90040
Part Number: G2964-90040 Rev. D
Edition 06/2015
Printed in Germany
© Agilent Technologies, Inc. 2012 - 2014, 2015
Agilent Technologies
Hewlett-Packard-Straße 8
76337 Waldbronn, Germany
Prepare TapeStation
1Launch the Agilent 2200 TapeStation software.
2Load Genomic DNA ScreenTape device and loading tips into the 2200 TapeStation.
Sample Preparation Genomic DNA ScreenTape Assay
1Equilibrate all reagents to room temperature for 30 min.
2Vortex mix before use.
3 Prepare ladder by mixing 10 µL Genomic DNA Sample Buffer ( ) with 1 µL Genomic DNA Ladder ( )
4Prepare sample by mixing 10 µL Genomic DNA Sample Buffer ( ) with 1 µL genomic DNA sample
(10 – 100 ng/µL).
5Spin down, then vortex using IKA vortexer and adaptor at 2000 rpm for 1 min.
6Spin down to position the sample at the bottom of the tube.
Sample Analysis
1Load samples into the 2200 TapeStation.
2Select the required samples on the controller software.
3Click Start and specify a filename with which to save your results.
Limited Use Label License
Some products within this system contain SYBR® , which is licensed from Molecular Probes Inc. for
use in research and development only. SYBR® is a registered trademark of Molecular Probes, Inc.
Technical Support
For technical support, please visit www.agilent.com/genomics/contact
Further Information
Visit Agilent Technologies` web site. It offers useful information, support and current developments
about the products and technology: www.agilent.com/genomics/tapestation
Parts required p/n Description
5067-5365 Genomic DNA ScreenTape
Parts required p/n Description
5067-5366 Genomic DNA Reagents
NOTE
Use a fresh ladder for each run. No electronic ladder is available for the Genomic DNA
assay.
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