Thermo Scientific CIMAREC BIOSYSTEM DIRECT User manual

Magnetic Stirrer
BIOSYSTEM DIRECT
BIOSYSTEM
Operating Manual
50134801 Issue 03.2012
TM
Directly and remote-controlled
single and multiple
magnetic stirring systems

Thermo Scientific 2 50134805_03.2012
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Contents
50134805_03.2012 3 Thermo Scientific
Table of Contents
1 User considerations...................................................................6
1.1 Correct use..........................................................................6
1.2 Incorrect use........................................................................6
1.3 Pictographs .........................................................................6
2 Safety considerations ................................................................7
3 Equipment description .............................................................10
4 Function...................................................................................11
5 Startup procedure....................................................................12
6 Stirring operation......................................................................14
6.1 Stirring vessels..................................................................14
6.2 Magnetic stirring bars........................................................15
6.3 Recommended rotation speed ranges ..............................18
6.4 Stirring...............................................................................18
7 Maintenance and cleaning.......................................................21
8 Troubleshooting.......................................................................22
9 Accessories .............................................................................24
10 Technical Specifications ..........................................................25
11 Warranty..................................................................................28

Assembly Drawings
Thermo Scientific 4 50134805_03.2012
Figure 1: Single magnetic stirring system BIOSYSTEM 1 with plug-in power supply unit.
*) Not included in scope of delivery.
Figure 2: Multiple magnetic stirring system BIOSYSTEM 4 B with control unit
BIOMODUL 40 B.
2
3
8
5
7
4
6
9
2
5
1
1
1 stirring device*
2 stirring vessel*
5 magnetic stirrer
7 control cable
8 power cable
9 control unit
1 stirring device*
2 stirring vessel*
3 power supply
4 secondary cable
5 magnetic stirrer
6 rotation speed
control button

Assembly Drawings
50134805_03.2012 5 Thermo Scientific
*) Not included in scope of delivery.
Figure 3: Control unit BIOMODUL 40 B, front view
Figure 4: Control unit BIOMODUL 40 B, back view
.
10 rotation speed indicator
11 stirring time control button
12 pause time control button
13 start/stop key
14 interval key
15 rotation direction key
16 Stirring power control
button
17 rotation speed control
button
18 power ON/OFF switch
19 power input
20 Output socket
10
11
13
12
14
15
17
16
18
19
20
5

1 User considerations
Thermo Scientific 6 50134805_03.2012
1 User considerations
1.1 Correct use
The magnetic stirring systems BIOSYSTEM DIRECT and BIOSYSTEM are designed
for stirring liquids in a laboratory or production environment. They are fitted with one,
three or four stirring points. They are specifically designed for stirring shear-sensitive
biological cultures without damaging the cells.
The stirring systems are suited to operation in the following areas:
on the laboratory bench,
in incubators,
in gas incubators (CO2incubators) with or without air humidification,
in cold chambers,
in laminar flow equipments,
in safety laboratories and sterile rooms,
directly controlled BIOSYSTEM: under water baths,
remote-controlled BIOSYSTEM in water baths.
1.2 Incorrect use
The magnetic stirring systems BIOSYSTEM DIRECT and BIOSYSTEM must not be
operated in hazardous locations.
Do not stir flammable liquids with a low boiling point.
The directly controlled magnetic stirring system BIOSYSTEM must not be operated in a
water bath.
1.3 Pictographs
You will find the following pictographs in this operating manual:


DANGER!
This sign refers to dangerous voltages.


DANGER!
This sign refers to hazardous situations.


CAUTION!
This sign indicates danger to equipment and machinery.


INFORMATION
This sign indicates easier working practices.
Indicates an operating step.


Indicates alternatives.

2 Safety considerations
50134805_03.2012 7 Thermo Scientific
2 Safety considerations
The device is compliant with the safety requirements of:
EN 61010
Low-voltage directive 73/23/EEC
EMC directive 89/336/EEC
For your own safety, you should observe the following safety warning signs.
The safety warnings indicate a possible source of danger.
At the same time they contain information on how correct action can avert danger. You
will always find warning signs attached to points of possible danger.


DANGER!
Magnetism.
Magnetic or metallic parts (e.g. data carriers, pacemakers, watches...)
can be affected by magnetic fields.
Keep such parts away from the magnetic stirrer (5) and the stirring
device (1).


DANGER!
Supply voltage and supply frequency must be within the range
specified for the power supply unit (3) or the control unit (9).


DANGER!
Defective secondary cables (4), power cables, or mains plugs must be
replaced only with original parts by the manufacturer or one of its
representatives.
Return the defective power supply unit (3) to our customer service for
repair.


DANGER
GLASS SPLINTER.
At high speeds of the mixing stick can lead to the destruction of the
sample vessels.
Wear safety goggles!
The vortex should not reach the stirring bar.
The stirring bar must rotate in the center of the sample vessel.
Watch the rotational behavior of the mixing stick for slow starts


CAUTION!
The equipment must not be operated in hazardous locations.

2 Safety considerations
Thermo Scientific 8 50134805_03.2012


CAUTION!
Permitted ambient conditions for the directly controlled BIOSYSTEM
DIRECT:
Magnetic stirrer (5): -10° to + 56°C at 100 % humidity.
Power supply unit (3): +10° to + 40°C at max. 80 % humidity.
The magnetic stirrer (5) must not be operated in a water bath.
The power supply unit (3) must not be run in humid rooms, or set up in
water splash zones.


CAUTION!
Permitted ambient conditions for the remote-controlled BIOSYSTEM :
Magnetic stirrer (5): -10° to + 56°C at 100 % humidity,
0° to + 50°C in a water bath.
Control unit (9): +10° to + 40°C at max. 80 % humidity.
The control unit (9) must not be run in humid rooms, or set up in water
splash zones.


CAUTION!
The magnetic stirring system must be switched off before connecting
or pulling the connector.
BIOSYSTEM DIRECT: The rotation speed control button (6) must
always be brought to position OFF first.
BIOSYSTEM: Switch off control unit (9) using power ON/OFF switch
(18).


CAUTION
High speed rotations of the stirring bar can cause a warming of the
goods.


CAUTION
At high speeds of the magnetic stirring, too high power setting can
lead to overheating of the stirred material.


CAUTION!
Do not place hot stirring vessels (2) on top of the magnetic stirrer (5).
Max. temperature: 56°C.


CAUTION!
Do not use cleaning agents which attack and corrode the stainless
steel surface of the magnetic stirrer (5).


CAUTION!
In case of repair, the equipment must only be opened by an authorised
service agent.

2 Safety considerations
50134805_03.2012 9 Thermo Scientific


CAUTION!
Do not allow the stirring device (1) to come to rest in an alternating
magnetic field if the device is unable to rotate. Do not subject the
stirring device (1) to a strong inverse magnetic field. This may cause
the device to become demagnetised.


CAUTION
Do not allow AlNiCo5 type magnetic stirring bars to remain in an
alternating magnetic field if they cannot rotate freely. Do not subject
the magnetic stirring bar to a strong inverse magnetic field. This may
cause the magnetic stirring bar to become demagnetized.


INFORMATION
The length of the magnetic stirring bar (2) must be smaller than the
diameter of the magnetic coupling (tech. specification).


INFORMATION
Check the box, the parts delivered on completeness and visible
damage.


INFORMATION
Use only appropriate sample containers! Review the sample containers
prior to use for visible damage (cracks, tears, splinters, leakages, etc.
...)


INFORMATION
Use only suitable and undamaged magnetic stirrers! Check the stirring
bar before use for signs of damage (cracks, chips ... etc.)
INFORMATION
Observe the relevant safety data sheets for chemical substances that
you are working in the magnetic stirrer.


INFORMATION
Read the instructions carefully before using the magnetic stirrer. No
application of the device by an untrained and inexperienced personnel.
INFORMATION
Use only in the instructions specified accessories, according to the
illustrated configuration.

3 Equipment description
Thermo Scientific 10 50134805_03.2012
3 Equipment description
Figures 1 to 5 show the components together with their position numbers.


INFORMATION
Check the box, the parts delivered on completeness and visible
damage.
The directly controlled magnetic stirring system BIOSYSTEM DIRECT is available as a
single or multiple magnetic stirrer (5) and a separate power supply unit (3).
The remote-controlled magnetic stirring system BIOSYSTEM is available as a single or
multiple magnetic stirrer (5) and a separate control unit (9).
The magnetic stirrer (5) is fitted with one, three or four stirring points. On the
BIOSYSTEM 4 DIRECT and 4, the four stirring points are arranged in a block.
Directly controlled BIOSYSTEM DIRECT:
Power is supplied via the power supply unit (3). A secondary cable (4) on the power
supply unit (3) is connected into the socket on the magnetic stirrer (5).
The rotation speed can be preset with the rotation speed control button (6). Following a
soft start, the stirring device (1) will rotate at the preset speed. To terminate the stirring
process, turn the rotation speed control button (6) slowly to position OFF. In this way
the rotation speed will be gradually reduced to zero.
Remote-controlled BIOSYSTEM:
The control cable (7) connects the magnetic stirrer (5) with the control unit (9)
BIOMODUL 40 B. The magnetic stirrer (5) is supplied with power and controlled via the
control unit (9). It is connected to the mains supply with the power cable (8).
The control unit (9) BIOMODUL 40 B has all the controls necessary for an effective
stirring operation:
rotation speed control button (17) with digital rotation speed indicator (10)
power adapted to the stirring task
reversal of rotation direction
continuous and interval operation
The stirring process is always started slowly and stopped slowly. The power ON/OFF
switch (18) for the control unit (9) BIOMODUL 40 B is located at the back of the unit..
The control unit (9) can drive up to two magnetic stirrers (5) simultaneously. Two output
sockets (20) are provided for this purpose at the back of the unit. The upper output
socket (20) is always used first.
A separate power supply for both equipment types ensures a safe low voltage situation
at the magnetic stirrer (5). Following a power failure, the magnetic stirrer (5) will come
on again automatically.
Drive and electronic control system are inside the polished stainless steel housing of
the magnetic stirrer (5), which is completely watertight. This prevents germs from
penetrating into the inside of the magnetic stirrer (5). The magnetic stirrer (5) is cleared
for use in safety laboratories and sterile rooms.
Stirring vessel (2) and stirring device (1) do not fall within the scope of delivery.

4 Function
50134805_03.2012 11 Thermo Scientific
4 Function
You can use the magnetic stirring systems BIOSYSTEM DIRECT and BIOSYSTEM to
stir liquids in one or several vessels simultaneously. They are used specifically with
respect to the following cultures and suspensions for gentle stirring in the laboratory
and in a production scale environment without damaging the cells:
cell suspensions,
tissue cultures,
microcarrier cultures,
culture baths.
You can stir the liquids in all commercially available culture flasks or bioreactors. As a
stirring device (1) you can use suspension-type stirring magnets, agitator blades,
paddles etc. capable of being moved by magnetic poles. Rotation speed control meets
the requirements of biotechnology and will always start slowly and stir without jerking.
When the stirrer is switched off, the rotation speed is slowly reduced to zero.
The stirring vessel (2) contains a suitable stirring device (1). When the stirring vessel (2)
is placed on the stirring point, a rotating magnetic field will move the stirring device (1).
Remote-controlled BIOSYSTEM :
The rotation speed is adjusted using the rotation speed control button (17) and
displayed on the rotation speed indicator (10).
You may choose between continuous stirring and interval operation. For interval
operation, set the required stirring and pause times on the control unit (9). The rotation
speed is reduced slowly at the end of every stirring period, and increased slowly at the
end of every pause. You can use the rotation direction key (15) to maintain or reverse
the direction of rotation after every pause.
Use the stirring power control button (16) to match the stirring power to the stirring task
in hand. This will keep the heat emitted to the stirred medium to a minimum. Heat
emission can also be reduced by working in the interval mode.
To stop and restart the stirring device (1), the control unit (9) is fitted with a start/stop
key (13). The rotation speed is slowly reduced, and then slowly increased again.

5 Startup procedure
Thermo Scientific 12 50134805_03.2012
5 Startup procedure


DANGER!
Supply voltage and supply frequency must be within the range specified
for the power supply unit (3) or the control unit (9).


DANGER!
Magnetism.
Magnetic or metallic parts (e.g. data carriers, pacemakers, watches...)
can be affected by magnetic fields.
Keep such parts away from the magnetic stirrer (5) and the stirring
device (1).


DANGER
GLASS SPLINTER.
At high speeds of the mixing stick can lead to the destruction of the
sample vessels.
Wear safety goggles!
The vortex should not reach the stirring bar.
The stirring bar must rotate in the center of the sample vessel.
Watch the rotational behavior of the mixing stick for slow starts


CAUTION!
The equipment must not be operated in hazardous locations.


CAUTION!
Permitted ambient conditions for the directly controlled BIOSYSTEM:
Magnetic stirrer (5): -10° to + 56°C at 100 % humidity.
Power supply unit (3): +10° to + 40°C at max. 80 % humidity.
The magnetic stirrer (5) must not be operated in a water bath.
The power supply unit (3) must not be run in humid rooms, or set up in
water splash zones.


CAUTION!
Permitted ambient conditions for the remote-controlled BIOSYSTEM:
Magnetic stirrer (5):-10° to + 56°C at 100 % humidity,
0° to + 50°C in a water bath.
Control unit (9): +10° to + 40°C at max. 80 % humidity.
The control unit (9) must not be run in humid rooms, or set up in water
splash zones.


CAUTION!
The magnetic stirring system must be switched off before connecting or
pulling the connector.
BIOSYSTEM DIRECT: The rotation speed control button (6) must always
be brought to position OFF first.
BIOSYSTEM: Switch off control unit (9) using power ON/OFF switch (18).

5 Startup procedure
50134805_03.2012 13 Thermo Scientific


INFORMATION
Read the instructions carefully before using the magnetic stirrer. No
application of the device by an untrained and inexperienced personnel.
Directly controlled BIOSYSTEM DIRECT:
Turn rotation speed control button (6) to position OFF.
Connect secondary cable (4) of power supply unit (3) into the jack at the back
of the magnetic stirrer (5).
Connect power supply unit (3) into mains socket.
If a germ-free environment is required, accommodate the power supply unit (3)
outside the sterile area.
Remote-controlled BIOSYSTEM:
Switch the power ON/OFF switch (18) at the back of the control unit (9) to
position OFF.
Connect the control cable (7) to the upper output socket (20) at the back of the
control unit (9).
When connecting a second magnetic stirrer:
Connect the control cable (7) of the second magnetic stirrer (5) to the lower
output socket (20) at the back of the control unit (9).
Connect the power cable (8) to the power input (19) on the control unit (9) and
into the mains socket.
If a germ-free environment is required, accommodate the control unit (9)
outside the sterile area.

6 Stirring operation
Thermo Scientific 14 50134805_03.2012
6 Stirring operation
6.1 Stirring vessels


CAUTION!
Do not place hot stirring vessels (2) on top of the magnetic stirrer (5).
Max. temperature: 56°C.
Use as stirring vessels (2) bioreactors or culture flasks.
When stirring larger volumes of liquid, you should use stirring vessels (2) having a
relatively small diameter with a thin bottom.
As a stirring device (1) you can use suspension-type stirring magnets, agitator blades,
pendulum type stirring devices, paddles etc. capable of being moved by magnetic
poles.
Figure 5: Stirring vessel with pendulum type stirring device.
If you use a pendulum type stirring device, you can vary the radius of the pendulum
motion: the closer the agitating globe is to the stirring drive, the greater will be the
radius of the pendulum motion (Figure 5, left). When you place the stirring vessel
further away from the stirring drive (e.g. by using a spacer under the stirring vessel), the
radius of the pendulum motion will be reduced (Figure 5, right).
Keep changing the distance between the stirring vessel (2) and the magnetic stirrer (5)
until you achieve an optimum pendulum motion.

6 Stirring operation
50134805_03.2012 15 Thermo Scientific
6.2 Magnetic stirring bars
INFORMATION
Use only suitable and undamaged magnetic stirrers! Check the stirring
bar before use for signs of damage (cracks, chips ... etc.)
Thermo recommends the following magnetic stirring bars:
Typ Size (mm) Material Order No.
KOMET 90 (Ø x L) 24 x 90 SmCo 50087902
Twin magnetic
stirring bar (L x B x H) 90 x 81 x 30 Rührstäbe: KOMET 90 SmCo 50087868
Magnetic stirring
bar with bearing (Ø x H) 146 x 48 stirring bar: KOMET 90 SmCo 50087869


INFORMATION
The length of the magnetic stirring bar (2) must be smaller than the
diameter of the magnetic coupling (tech. specification).


INFORMATION
Some magnetic stirring bars (2) (especially triangular bars) may have a
critical resonance frequency at lower rotation speeds. This may cause
the magnetic stirring bar (2) to wander away from the turning center and
carry out periodic oscillations. Avoid this rotation speed setting when
the problem occurs. Quickly travel through this problem range when
adjusting the rotation speed.
Thermo has developed a new magnetic stirring bar KOMET 90
(Figure 2). It contains a high-quality super-strong samarium-cobalt magnet.
KOMET 90 shows a strong magnetic force. The stronger attraction to the magnetic
alternating field provides the stirring bar with very efficient stirring properties. Even if
there are greater distances (e.g. in high measuring cylinders) the stirring force will be
maintained. The stability of the stirring bar will not be impaired if the vessel has a
curved bottom. The strong magnetic coupling in samarium-cobalt magnets will also
increase friction. The standard design of the KOMET magnetic stirring bar is therefore
unsuitable for stirring liquids containing particulate matter (such as mud) or for stirring in
vessels with a rough interior surface. Some of the KOMET stirring bars were specially
fitted with a wear-resistant glide ring for use under excruciating circumstances, such as
when stirring vessels feature rough plastic or stainless-steel bottoms or if the media to
be stirred contain solids (Figure 3).
Any risk of demagnetization by external magnetic fields is completely eliminated. All
KOMET series stirring bars can be readily recognized by their two conical ends.

6 Stirring operation
Thermo Scientific 16 50134805_03.2012
Figure 1: Stirring bar KOMET 90 (Order No. 50087902).
Side view (left) and cross-section through central portion (right).
Figure 2: KOMET stirring bar with glide ring
If you are working with stirring vessels having rough plastic or stainless steel surfaces,
you should use a twin magnetic stirring bar (suspension not included in scope of
delivery). It is equipped with two magnetic stirring bars KOMET 90. The stirring bar is
suspended from the top into the stirring vessel and kept away from the bottom (Figure
4). Alternatively, it can be fixed at the bottom of the stirring vessel with the aid of a
centering device (centering device not included in scope of delivery).

6 Stirring operation
50134805_03.2012 17 Thermo Scientific
10 mm
10 mm
Figure 3: Twin magnetic stirring bar (left, Order No. 50087868).
Cross-section through stirring bar suspended from top (centre), or fixed at the
bottom of the stirring vessel (right).
In addition, a magnetic stirring bar incorporating a bearing can be supplied as an
accessory item (Figure 4). It can be kept in place safely inside the stirring vessel.Only
use this stirring bar at lower or medium rotation speed.
Figure 4: Magnetic stirring bar with bearing (Order No. 50087869)
To improve the slide characteristics of the magnetic stirring bar, you can place a stirring
disc into the stirring vessel (Figure 6). It consists of a flat glass or PTFE disc and a
screen. The latter prevents the magnetic stirring bar from escaping in a sideways
direction. When using the stirring disc, take care to note that it will not rotate of its own
accord. Affix it to the bottom of the vessel, for example.
Figure 5: Stirring disc with screen

6 Stirring operation
Thermo Scientific 18 50134805_03.2012
6.3 Recommended rotation speed ranges
We recommend the following rotation speed ranges for various applications:
6.4 Stirring


DANGER!
Magnetism.
Magnetic or metallic parts (e.g. data carriers, pacemakers, watches...)
can be affected by magnetic fields.
Keep such parts away from the magnetic stirrer (5) and the stirring
device (1).


DANGER
GLASS SPLINTER.
At high speeds of the mixing stick can lead to the destruction of the
sample vessels.
Wear safety goggles!
The vortex should not reach the stirring bar.
The stirring bar must rotate in the center of the sample vessel.
Watch the rotational behavior of the mixing stick for slow starts


CAUTION
High speed rotations of the stirring bar can cause a warming of the
goods.
 CAUTION
High speed rotations of the stirring bar can cause a warming of the
goods.
Application Speed range (rpm)
Microbiological and biotechnical applications:
Aeration of bacteria cultures
Growth of bacteria cultures
Dissolving nutrient mediums, intense aeration of
bacteria cultures
200 - 350
300 - 450
350 - 500
Routine laboratory work:
Prevent accretion of suspended matter
Titration
Dissolving solids
Improved heat transmission
Chemical reactions
150 - 250
250 - 400
350 - 700
300 - 600
100 - 1000

6 Stirring operation
50134805_03.2012 19 Thermo Scientific


CAUTION!
Do not place hot stirring vessels (2) on top of the magnetic stirrer (5).
Max. temperature: 56°C.


INFORMATION
Use only suitable and undamaged magnetic stirrers! Check the stirring
bar before use for signs of damage (cracks, chips ... etc.)


INFORMATION
Observe the relevant safety data sheets for chemical substances that
you are working in the magnetic stirrer.
Directly controlled BIOSYSTEM:
Fill the stirring vessel (2).
Suspend the stirring device (1) into the stirring vessel (2).
Turn the rotation speed control button (6) to position OFF.
Center the stirring vessel (2) onto the stirring point.
Set the desired rotation speed with the rotation speed control button (6).
The stirring device (1) will start rotating slowly. At the end of the start-up phase, the
stirring device will run at the preset rotation speed.
To stop the stirring process, turn the rotation speed control button (6) to
position OFF.
Remote-controlled BIOSYSTEM:
Fill the stirring vessel (2) and center it onto the stirring point.
Suspend the stirring device (1) into the stirring vessel (2).
Switch on the control unit (9) with the power ON/OFF switch (18).
The green LED display in the start/stop key (13) will light up.
Push the start/stop key (13) twice, and hold it down.
Now set the desired rotation speed with the rotation speed control button (17).
The set rotation speed is immediately displayed on the digital rotation speed indicator
(10).
Continuous operation:
Lock the interval key (14) to position NORMAL.
The yellow LED display in the interval key (14) will not light up.
Interval operation:
Lock the interval key (14) to position INTERVAL.
The yellow LED display in the interval key (14) will light up.
Set the desired stirring time with the stirring time control button (11).
Set the desired pause time with the pause time control button (12).
The rotation speed is reduced slowly at the end of every stirring period, and
increased slowly at the end of every pause. You can choose to maintain or
reverse the direction of rotation after every stirring interval.
If you want to reverse the direction of rotation, lock the rotation direction key
(15) to position STIR.
The yellow LED display in the rotation direction key (15) will light up.

6 Stirring operation
Thermo Scientific 20 50134805_03.2012
If you do not want to reverse the direction of rotation, lock the rotation direction
key (15) to position SPIN.
The yellow LED display in the rotation direction key (15) will not light up.
Power adaptation:


CAUTION
At high speeds of the magnetic stirring, too high power setting can lead
to overheating of the stirred material.
High stirring power for large stirring volumes, high rotation speed, viscous
mediums, and when several stirring drives are driven simultaneously,
Low stirring power for small stirring volumes, low rotation speed, mediums
resembling water, and when there is a requirement for heating the standing
surface, or heat emission in the incubator.
Set the desired stirring power with the stirring power control button (16).
Take care to note the turning motion of the stirring device (1). It should turn evenly
and smoothly without jerking.
Manual stop and stirring restart:
Hold down the start/stop key (13).
The rotation speed will be slowly reduced to zero.
Release the start/stop key (13).
The rotation speed will be slowly increased to the preselected rotation speed.
If you want to terminate the stirring process:
Switch off the control unit (9) using the power ON/OFF switch (18).