Turner Designs CYCLOPS 7 User manual

www.turnerdesigns.com
998-0270 Revision J Page 1 of 2
Quick Start Guide
“A” = PTSA“A” = PTSA
Congratulations on the purchase of your new Cyclops Submersible Sensor. We are committed to customer
satisfaction. If you need assistance, technical specialists are available to answer your questions at 408-749-0994 or
toll-free at 877-316-8049. This Quick Start Guide will help you set up your Cyclops Submersible Sensor and describe
how to take measurements so you can start collecting data as quickly as possible.
How to identify for which fluorophore your Cyclops is configured:
“C” = Chlorophyll “R” = Rhodamine WT
“F” = Fluorescein “P” = Phycocyanin
“E” = Phycoerythrin “U” = CDOM / fDOM
“O” = Crude Oil “B” = Optical Brighteners
“T” = Turbidity “A” = PTSA
“G” = Refined Fuels
Figure 1
Initial Connections
Attach the 6-pin female connector to the sensor and connect the wire leads as shown in Figure 1.
Do not connect or ground the Brown and Blue wires at this time.
Leaving them disconnected will set the sensor to the X1 gain range.
Functional Test Validation
With the Cyclops connected as shown in Figure 1, make the following functional checks:
• The LED is on
• The multimeter reads >0 VDC
• Moving the light source closer to your hand causes the output voltage to increase.
12.00 .015 + -
DC Power Supply
PSU Positive Connection
(Red) Supply Ground 0 VDC (Black)
3.52
Multimeter
10 VDC
+-
Signal Output
“+” (White)
Analog Ground
“-” (Green)
Blue
(Tie to Green for
10X)
Brown
(Tie to Green for
100X)
X1(Low) Gain
Leave both wires
disconnected
for Functional Check
Cyclops Pigtail Cable
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops Pigtail cable holes
33
66
44
22
1
55 5
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
33
44
22
1
55
6
6
33
44
22
1
55
6
6
33
44
22
1
55
6
6
Figure 1
Initial Connections
Attach the 6-pin female connector to the sensor and connect the wire leads as shown in Figure 1.
Do not connect or ground the Brown and Blue wires at this time.
Leaving them disconnected will set the sensor to the X1 gain range.
Functional Test Validation
With the Cyclops connected as shown in Figure 1, make the following functional checks:
• The LED is on
• The multimeter reads >0 VDC
• Moving the light source closer to your hand causes the output voltage to increase.
12.00 .015 + -
DC Power Supply
PSU Positive Connection
(Red) Supply Ground 0 VDC (Black)
3.52
Multimeter
10 VDC
+-
Signal Output
“+” (White)
Analog Ground
“-” (Green)
Blue
(Tie to Green for
10X)
Brown
(Tie to Green for
100X)
X1(Low) Gain
Leave both wires
disconnected
for Functional Check
Cyclops Pigtail Cable
12.00 .015 + -
DC Power Supply
12.00 .015 + -
DC Power Supply
PSU Positive Connection
(Red) Supply Ground 0 VDC (Black)
3.52
Multimeter
10 VDC
+-
3.52
Multimeter
10 VDC
+-
Signal Output
“+” (White)
Analog Ground
“-” (Green)
Blue
(Tie to Green for
10X)
Brown
(Tie to Green for
100X)
X1(Low) Gain
Leave both wires
disconnected
for Functional Check
Cyclops Pigtail Cable
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops Pigtail cable holes
33
66
44
22
1
55 5
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
33
44
22
1
55
6
6
33
44
22
1
55
6
6
33
44
22
1
55
6
6

www.turnerdesigns.com
998-0270 Revision J Page 2 of 2
Quick Start Guide
Gain Switching Table
*Gain switching table includes a subset of the available applications.
0-1000-100-5X100
Connected
to analog
ground
Not
connected
0-10000-1000-50X10
Not
connected
Connected
to analog
ground
0-30000-1,0000-500X1
Not
connected
Not
connected
TRB Range
(NTU)
RWT
Range
(ppb)
Chl
Range
(g/L)
GainGain X100
(Brown)
Gain
X10
(Blue)
Choosing the Right Gain Setting
The gain setting refers to the sensitivity adjustment of the sensor. There are three gain settings – X1, X10 and X100.
As the gain increases, the sensitivity increases and the measuring range decreases. If integrating Cyclops sensors
with a datalogger or instrumentation that doesn’t have dynamic gain control, users must determine which gain to use
prior to deployment. In most cases an integration cable is made to activate a specific gain (refer to the User’s Manual).
If you want the ability to utilize all three gains, your datalogger or instrumentation needs to have dynamic gain control
as a built-in function or programmable outputs (refer to the User’s Manual).
In most instances the X10 gain will provide the appropriate sensitivity and range. If you are working in very low
concentration applications (<2 μg/L chl or <5 ppb rhodamine WT), the X100 gain is recommended. If very high
concentrations are expected (>40 μg/L chl or >80 ppb rhodamine WT) the X1gain is recommended.
If you are uncertain of which gain setting to use you can take readings of a representative sample of water in the
laboratory and determine which gain is the most appropriate.
Gain Determination Procedure
1. For in vivo chlorophyll applications, take a natural sample of water from a sampling station where you plan to deploy
the Cyclops. Applying good measurement practices, store it properly and quickly transport it to a laboratory where
you have the Cyclops connected to a multimeter and DC power source (see Figure 1).
2. Pour the water sample into a clean glass beaker and submerge the optical end of the Cyclops (See Sample
Analysis below).
3. Activate the X10 gain setting (see Gain Switching Table above). If you believe the sample represents typical
concentrations (i.e. not a bloom or other abnormal event) obtain a signal from the sample. The signal should be
significantly higher than a blank sample (DI water or filtered seawater), but not close to the 5 volt maximum.
4. If the sample signal is high, (>3.0 V for example) you may choose to use the X1 gain instead of the X10 gain setting
so that you avoid going over scale once you deploy the Cyclops.
5. If the sample signal is very low (<0.3V) you may choose to use the X100 gain setting to achieve higher sensitivity but
a smaller measuring range.
This process is easier for dye tracing applications because users can create specific concentrations that represent typical
or expected concentrations encountered during sampling to determine the appropriate gain.
Sample Analysis
When using the Cyclops in the laboratory it is important to be aware of the following points:
1. When using the Cyclops with discrete samples, allow at least 3” of clearance from the bottom of a sample container.
2. Ensure that there are no reflective or light colored surfaces under the sample containers. White or reflective surfaces
will result in an elevated signal.
3. Check the optical surface of the sensor to ensure it is free from bubbles.
Gain Switching Table
*Gain switching table includes a subset of the available applications.
Gain Switching Table
*Gain switching table includes a subset of the available applications.
0-1000-100-5X100
Connected
to analog
ground
Not
connected
0-10000-1000-50X10
Not
connected
Connected
to analog
ground
0-30000-1,0000-500X1
Not
connected
Not
connected
TRB Range
(NTU)
RWT
Range
(ppb)
Chl
Range
(g/L)
GainGain X100
(Brown)
Gain
X10
(Blue)
Choosing the Right Gain Setting
The gain setting refers to the sensitivity adjustment of the sensor. There are three gain settings – X1, X10 and X100.
As the gain increases, the sensitivity increases and the measuring range decreases. If integrating Cyclops sensors
with a datalogger or instrumentation that doesn’t have dynamic gain control, users must determine which gain to use
prior to deployment. In most cases an integration cable is made to activate a specific gain (refer to the User’s Manual).
If you want the ability to utilize all three gains, your datalogger or instrumentation needs to have dynamic gain control
as a built-in function or programmable outputs (refer to the User’s Manual).
In most instances the X10 gain will provide the appropriate sensitivity and range. If you are working in very low
concentration applications (<2 μg/L chl or <5 ppb rhodamine WT), the X100 gain is recommended. If very high
concentrations are expected (>40 μg/L chl or >80 ppb rhodamine WT) the X1gain is recommended.
If you are uncertain of which gain setting to use you can take readings of a representative sample of water in the
laboratory and determine which gain is the most appropriate.
Gain Determination Procedure
1. For in vivo chlorophyll applications, take a natural sample of water from a sampling station where you plan to deploy
the Cyclops. Applying good measurement practices, store it properly and quickly transport it to a laboratory where
you have the Cyclops connected to a multimeter and DC power source (see Figure 1).
2. Pour the water sample into a clean glass beaker and submerge the optical end of the Cyclops (See Sample
Analysis below).
3. Activate the X10 gain setting (see Gain Switching Table above). If you believe the sample represents typical
concentrations (i.e. not a bloom or other abnormal event) obtain a signal from the sample. The signal should be
significantly higher than a blank sample (DI water or filtered seawater), but not close to the 5 volt maximum.
4. If the sample signal is high, (>3.0 V for example) you may choose to use the X1 gain instead of the X10 gain setting
so that you avoid going over scale once you deploy the Cyclops.
5. If the sample signal is very low (<0.3V) you may choose to use the X100 gain setting to achieve higher sensitivity but
a smaller measuring range.
This process is easier for dye tracing applications because users can create specific concentrations that represent typical
or expected concentrations encountered during sampling to determine the appropriate gain.
Sample Analysis
When using the Cyclops in the laboratory it is important to be aware of the following points:
1. When using the Cyclops with discrete samples, allow at least 3” of clearance from the bottom of a sample container.
2. Ensure that there are no reflective or light colored surfaces under the sample containers. White or reflective surfaces
will result in an elevated signal.
3. Check the optical surface of the sensor to ensure it is free from bubbles.