UGO BASILE 47883 User manual

Instruction manual
Librae Incapacitance tester
July 2020 revision 2.2
SKU: 47883/ 47882/ 47885/ 47880-001
Pain
Inflammation

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SAFETY
CONSIDERATIONS
Your science, our devices
More than 30.000 citations
The Librae Incapacitance Tester has
been designed and approved in
accordance with international safety
standards.
Please follow the safety instructions,
cautions and warnings in this manual
to ensure safe operation and to retain
the instrument in a safe condition.
The instrument cover must not be
opened or removed. Capacitors inside
the instrument may be charged, even
even ater it has been disconnected
from all power sources.
Product service and repair must be
performed by qualified personnel
authorized by the Ugo Basile
organization.
Instruction Manual
July 2020 Revision 2.2
MOD. 13 Rev. 1
CE CONFORMITY STATEMENT
Manufacturer UGO BASILE srl
Address Via G. di Vittorio, 2 – 21036 Gemonio, VA, ITALY
Phone n. [phone]
Fax n. [phone]
Email [email protected]
We hereby declare that
Instrument. Librae Incapacitance tester for mice and rats
SKU 47883/47882/47885/47880-001
is manufactured in compliance with the following European Union Directives
and relevant harmonized standards
• 2006/42/CE on machinery
• 2014/35/UE relating to electrical equipment designed for use within certain voltage limits
• 2014/30/UE relating to electromagnetic compatibility
• 2011/65/UE and 2015/863/UE on the restriction of the use of certain hazardous substances in
electrical and electronic equipment
Account Manager Mauro Uboldi
Nome / Name
July 2020
Date Firma / Signature

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Librae instruction manual > Page: 5
Thank you for your purchase of Librae
Incapacitance Tester for measuring hind paw
weight distribution using the most widely
used method (>1,500 citations).
Your new instrument is designed with latest
technologies to provide researchers with
important features and benefits:
• Touch screen with Autostart button
lets scientists set, control and manage
instrument data quickly and seamlessly.
• The supplied USB key stores data for
optimal portability (average paw weight,
Standard Deviation, L/R ratio, etc....).
• The Let/Right weight histograms and
scatter charts display experiment output
for monitring data trends in real time.
•
• Reliable data: high quality force sensor
(0.1g resolution) is easily calibrated using
the reference weight supplied.
• There are no screws or protruding parts
that may interfere with animal comfort,
maximising experiment repeatability.
• Ater each test, results can be stored
or discarded. Experiment data can be
transferred to PC spreadsheets such as
Microsot Excel.
• Magnetic foot pads can be easily detached
for fast cleaning within seconds.
A range of related products is featured at the
end of this manual and also at ugobasile.com
Product features What is in the box?
Librae package contents::
SKU: 47883
The Librae Incapacitance tester for mice
1 transparent plastic restrainer for mice
1 Pedal switch
1 Power cord in accordance with your country’s requirements
1 Calibration weight
1 USB key containing a copy of:
This instruction manual
Quality control and warranty certificate
SKU: 47882
The Librae Incapacitance tester for rats
1 transparent plastic restrainer for rats
1 Pedal switch
1 Power cord in accordance with your country’s requirements
1 Calibration weight
1 USB key containing a copy of:
This instruction manual
Quality control and warranty certificate
SKU: 47885
The Librae Incapacitance tester for mice and rats
1 transparent plastic restrainer for mice
1 transparent plastic restrainer for rats
1 Pedal switch
1 Power cord in accordance with your country’s requirements
1 Calibration weight
1 USB key containing a copy of:
This instruction manual
Quality control and warranty certificate
Additional optional items:
SKU: 47880-003
Librae Incapacitance tester restrainer for mouse in clear perspex, width 40 mm
SKU: 47880-002
Librae Incapacitance tester restrainer for rat in clear perspex, width 64 mm
SKU: 47880-320
Librae Incapacitance tester calibration weight 100 gr.
SKU: 47880-321
Librae Incapacitance tester magnetic pads (pair let + right)

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1 General Librae instruction manual > Page: 7
1 General ................................................................................7
1.1 Principle of Operation...........................................................7
2 Instrument Description ..................................................................7
2.1 Main Unit......................................................................7
2.1.1 Important Notice about Force Range .............................................7
2.1.2 Animal Welfare................................................................8
2.1.3 Touch-screen command/display ................................................8
2.1.4 Notes on Resistive Touch-Pads..................................................9
2.2 Animal Restrainer ..............................................................9
2.3 Librae Features ................................................................9
3 Installation............................................................................10
3.1 Unpackaging & Preliminary Check................................................10
3.2 Note on the Instruction Manual .................................................10
3.3 Safety Instructions ............................................................10
3.4 Assembling the instrument .....................................................11
3.5 Before Applying Power .........................................................11
3.6 Intended Use .................................................................12
3.7 Additional Safety Considerations ................................................12
3.8 Connection...................................................................12
4 Operation .............................................................................14
4.1 Home Page ...................................................................14
4.1.1 Quick setup for first test.......................................................16
4.1.2 System Icons ................................................................17
4.1.3 Home Page Icons.............................................................18
4.2 Experiment ...................................................................18
4.3 Setup........................................................................20
4.4 Start.........................................................................21
4.5 Calibration ...................................................................22
4.6 Set Autostart .................................................................23
4.7 Utilities ......................................................................26
4.7.1 Update......................................................................26
4.7.2 Date-Time ...................................................................27
4.7.3 Advanced Settings............................................................27
4.7.4 Erase DB ....................................................................28
4.7.5 Results .....................................................................28
4.7.6 USB Storage .................................................................29
4.8.1 Communication port connection ...............................................30
5 Maintenance ..........................................................................32
5.1 Electrical .....................................................................32
5.2 Cleaning/disinfection ..........................................................32
5.3 Long Inactivity ................................................................32
5.4 Customr Support ..............................................................32
6 Specifications .........................................................................32
Contents 1 General
The Librae Incapacitance Tester can be used for a number of applications that require animal
weight bearing measurement (hind paw weight distributions).
The most common application is for joint pain studies and related drug candidates, especially
for osteoarthritis.
1.1 Principle of Operation
The Librae Incapacitance Tester provides weight bearing measurement of restrained rats or
mice, by using two force sensors, one per paw. These independently measure the paw weight
over time (the weight averaged over a few seconds is normally used as the data point).
For example, the device provides an index of discomfort in the osteoarthritic knee, normally
expressed as the Let/Right weight ratio or Let/Right weight dierence between the two paws.
2 Instrument Description
The Instrument comprises:
• The main unit, an original Ugo Basile design, see paragraph 2.1, incorporating the 2 force
sensors, touch screen controls and a display screen for viewing data trends and results
• A Plexiglas restrainer for mouse or rat (or both depending on the model), see paragraph 2.2.
• A pedal switch to start the experiment (or use touch screen to start the experiment)
The Librae Incapacitance Tester provides data files (CSV) that can be transferred to a PC for
analysis using a spreadsheet application such as Microsot Excel.
Data is also saved automatically (e.g. let and right weights, L/R ratio, etc...) and can be retrieved
using the touch screen.
2.1 Main Unit
The measurement plate on the unit is a 10mm aluminium slab, to ensure maximum thermal
and mechanical stability.
High quality force sensors provide the measurement with a resolution of 0.1g. Foot pads are
magnetically attached to make them easy to remove and clean ater every experiment. The foot
pads are flat without any physical protrusions that could cause negative eects on the comfort
or gait of the animal.
Four rubber feet act as dampeners in order to absorb small vibrations from the work bench or
the environment.
2.1.1 Important Notice about the Force Range
The maximum weight the instrument can carry is 2’200g per paw.
Applied forces that are greater than 2’200g per paw, will not be measured.
A mechanical limit switch, avoids a force overload greater than 4’400g.
Do not place animals weighing more than 5’000g on the Librae Incapacitance Tester to avoid
causing any permanent damage to the instrument.

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2 Instrument Description 2 Instrument Description Librae instruction manual > Page: 9
2.1.2 Animal Welfare
In any analgesia test, great care must be taken to prevent the animal from experiencing
any inadvertent harm.
When an investigator initially begins using the incapacitance test, and when first
assessing a new strain of animal, care must be taken to identify the appropriate
amount of stimulus that will produce the desired response.
2.1.3 Touch-screen command/display
Librae incorporates a 4.3” touch-screen display, for basic setting and monitoring, via an
intuitive control panel.
Figure 2.1.3.1, “Main Menu”
During the test (Figure 2.1.3.2), the touch-screen display indicates the actual force applied on
each foot pad, the duration of the experiment and the function mode.
Figure 2.1.3.2, Measurement page
The test results, can be browsed in a condensed view, as shown in Figure 2.1.3.3.
Figure 2.1.3.3, “Results Page”
2.1.4 Notes on Resistive Touch-Pads
If you compare the touch sensitivity of your tablet or smart-phone with the Librae touch-screen,
you may notice a dierent, less sensitive response. You may need to press slightly harder, or
use a stylus pen on the touch screen. This is because we employ a resistive touch-screen dis-
play (while most smart phones use a capacitive screen).
Resistive screens are a better choice for our application due to their:
• High resistance to dust and water
• Ability to work with gloved hands or stylus (unlike capacitive technology which is dependent
on the conductive nature of human body and doesn’t work if the user is wearing gloves).
2.2 Animal Restrainer
The animals are confined by a clear removable restrainer made of clear Perspex. Two dierent
versions (mouse and rat) are available. The top lid helps easy removal of the animal when the
experiment is finished.
Restrainers with custom measurements can be made on request. Please send an email
to: [e-mail]
2.3 Librae Features
The main features of Librae Incapacitance Tester are:
1. Force range: from 20g to 2200g per paw, with a 0.1g resolution over the full scale
2. Manual and Autostart mode
3. Detection by pedal switch of elapsed time-out
4. Visualization averaging for smooth reading without aecting the sample rate
5. Histogram and Scroll Graph of applied force.
6. Data portability via USB memory card (.CSV file format)

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3 Installation 3 Installation Librae instruction manual > Page: 11
3 Installation
3.1 Unpacking & Preliminary Check
Check the contents of your shipment (see “What is in the box?” page at the start of this manu-
al), and visually inspect the instrument as you take it out of the packaging.
If the instrument appears to be damaged, inform the carrier immediately and send an email to
[e-mail]
If ater having tested the instrument, it fails to meet normal performance standards, please
contact our ater sales service, see paragraph 5.4-Customer Support.
Protect the environment:
Please dispose of packaging according to existing and applicable waste management rules and
regulations.
3.2 Note on the Instruction Manual
This Instruction Manual included in the box (on the USB pen drive in a PDF form) is necessary
for the correct installation and operation of the instrument.
We recommend reading the manual carefully, as it is essential for understanding the correct
installation and operation of the instrument.
Please save the manual, ready for consultation by the qualified personnel using the instrument.
Print it, only if necessary.
Our Instruction Manuals are available as free downloads on our web site, www.ugobasile.com
For any additional information and/or assistance, you are welcome to contact our Service
Department (see paragraph 5.4-Customer Support), specifying the serial number of your
instrument.
3.3 Safety Instructions
The following guidelines must be followed to ensure safe operation.
DO NOT attempt to open the cover or perform any service work
DO NOT connect human subjects to the device
3.4 Assembling the instrument
Place the instrument on a stable and flat surface where it will be used for tests.
Adjust the four rubber feet until the instrument is level.
Position the mouse or rat restrainer onto the base plate.
Do not lit or move the combined instrument when assembly is completed.
Do not incline the device, as the Perspex restrainer may accidentally fall o the plate. Please
do not block the lateral ventilation grids.
For proper performance, ensure there is a free space of 20cm on the let, right and rear of the
instrument.
Figure 3.4.1
3.5 Before Applying Power
The Power Module (see figure 3.5.1) is positioned on the let hand side of the back panel,
which encompasses – from let to right - the fuse holder, the main ON/OFF switch and the
inlet connection of the power cord.
Figure 3.5.1
The two-pole toggle switch, which complies with international safety standards, allows you to
turn the instrument ON or OFF.
The fuse holder uses two fuses. Use (T400mA) slow-blow fuses for operation at both 115 or 230
Volts, for fuse replacement, please refer to paragraph 5.1-Electrical.
The power cord inlet fits a standard C13 cable, Cat. # E-WP008. Ensure your power out-take is
provided with a reliable ground connection.

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3 Installation 3 Installation Librae instruction manual > Page: 13
3.6 Intended Use
The Librae Incapacitance Tester is intended for investigation use on laboratory animals only.
3.7 Additional Safety Considerations
1. Use Ugo Basile accessories and parts only
2. Immediately disconnect and replace a damaged power cord
3. Do not obstruct easy access to the power module
4. Do not obstruct the grids on the sides of the cabinet, see paragraph 3.4
5. Do not operate in hazardous environments or outside prescribed environmental limita-
tions, see paragraph 6.0
6. Do not spray any liquid on the connectors, display, or other parts
Ugo Basile cannot in any way and form be held responsible for damage caused to things and
people due to:
• Incorrect electrical supply
• Incorrect installation procedure
• Incorrect or improper use or, in any case, not in accordance with the purpose for which the
instrument has been designed and the warnings stated in the instruction manual supplied
with the instrument
• Replacement of original components, accessories or parts with others not approved by the
manufacturer
• Servicing carried out by unauthorized personnel
See also paragraphs 2.1.1 and 2.1.2
3.8 Connection
Connect the mains cord between the power socket of the Librae and the power outlet, provided
with a reliable earth connection.
Connect the pedal switch to the Librae Incapacitance Tester (blue bushing Figure 3.8.1). Note
that the connector of the pedal switch is provided with a polarization key; align it with the blue
bushing, and gently push in.
To remove the connector, gently pull out.
DO NOT ROTATE THE CONNECTOR: PERMANENT DAMAGE WILL OCCUR
Figure 3.8.1
The connection module on the front panel (Figure 3.8.2) encompasses the following
connectors, from let to right:
1. Upper and lower USB ports: enable data exchange (configurations & results) with a PC, and
allows firmware upgrades, via the USB flash drive provided in the box (see paragraph 4.7.1)
2. TTL I/O: 15pins D-SUB connector, provides TTL input and output for start/stop command
and mode settings (see paragraph 4.8.1 for pin-out configuration)
3. COM: Reserved for maintenance and service purpose
4. Ethernet connector: Reserved, not to be used
Figure 3.8.2
USB Hard Disk
If the internal memory is corrupted, the Instrument will ask you to insert a USB memory key
into the lower USB port. This allows you to run the experiment.
The instrument needs to be checked by UGO BASILE personnel for maintenance.

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4 Operation 4 Operation Librae instruction manual > Page: 15
4 Operation
Turn the instrument ON by using the ON/OFF power switch at the rear of the device. The
instrument starts up and performs a system check, which might take up to 60 seconds. During
this time the UB logo and product name will be displayed (Figure 4.0.1)
Figure 4.0.1
4.1 Home Page
When the start-up is finished, the Home Page Menu is displayed (Figure 4.1.1 and 4.1.2).
Figure 4.1.1 - Main Menu, with UB logo Home Page
Figure 4.1.2 - Utilities Main Menu, with folder logo Home Page
On the home page, press the “UB logo” button to view sotware and memory information, as
shown in Figure 4.1.3. Press the “UB logo”again to return to main menu page.
Figure 4.1.3

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4 Operation 4 Operation Librae instruction manual > Page: 17
4.1.1 Quick setup for the first test
You are now ready to configure and run your first experiment.
The following steps will guide you to quickly configure Librae Incapacitance Tester and get your
first results:
1. Press the SETUP icon to configure your experiment (Refer to paragraph 4.3)
2. Press the EXPERIMENT icon to fill in protocol, treatment, animal ID, etc... (Refer to paragraph
4.2)
3. If desired, configure Autostart parameters to operate with Autostart mode (Refer to para-
graph 4.6)
4. Ensure that no animal (or other load) is present on the foot pads, and press START icon
(Refer to paragraph 4.4)
5. Place the animal restrainer in the correct position.
6. To let the animal enter the restrainer, tilt the restrainer upwards to open the rear. The
restrainer includes a hole for the rodent tail so that it does not become enclosed.
7. Ater the animal has entered the restrainer, tilt the restrainer back down to the flat position
8. When ready, press START button (or pedal switch) to run the experiment
6.1 If Autostart Mode is enabled, wait for the instrument trigger for automated start.
Refer to paragraph 4.6 for more details.
9. At the end of an experiment, a result window is displayed: you can accept or reject the
result (Refer to paragraph 4.7.5).
Your experiment is complete, and Librae is ready to perform a new measurement
4.1.2 System Icons
System icons are shown in Figure 4.1.1.1.
Figure 4.1.1.1
1. Date and Time: shows the current date and time. To modify it, go to UTILITIES (see
paragraph 4.7.2).
2. UB Logo: shows sotware and memory information (see paragraph 4.1).
3. Update Firmware: Librae Incapacitance Tester is ready to install the update. See paragraph
4.7.1 for details.
4. USB memory stick:
4.1 Grey: no USB stick detected (default)
4.2 Green up: Upper USB memory stick detected and enabled
4.3 Green down: Lower USB memory stick detected and enabled
5. SD internal memory:
5.1 Blue: SD card detected and enabled (default)
5.2 Grey: SD card failure
6. Scale icon:
6.1 Grey: no acquisition in progress (default)
6.2 Blue: acquisition in progress

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4 Operation 4 Operation Librae instruction manual > Page: 19
4.1.3 Home Page Icons
Figure 4.1.1 - Main Menu, with UB logo Home Page
Main menu page (Figure 4.1.1) gives you access to the following commands:
1. Experiment: Enter information about the test, including treatment, protocol, stage, trials
(see paragraph 4.2)
2. Setup: Configure the weight of the animal, experiment duration, and function mode (see
paragraph 4.3)
3. Start: Perform the experiment (see paragraph 4.4)
4. Calibration: Calibrate the Librae Incapacitance Tester (see paragraph 4.5)
5. Set Autostart: Configure the autostart parameters (see paragraph 4.6)
6. Utilities: Change data and time, erase the database, copy the experimental results into a
USB memory stick, and more. See paragraph 4.7 for more details.
4.2 Experiment
Figure 4.2.1 - Experiment form page
TREATMENT: treatment or drug delivered to the animal.
Depress the text field to enable a virtual keyboard (Figure 4.2.2).
This is an alphanumeric field: enter a maximum of 12 characters.
Figure 4.2.2 - Characters Virtual Keyboard
Figure 4.2.3 - Numeric Virtual Keyboard
PROTOCOL: name of the applied protocol. Depress the text field to enable a virtual keyboard.
This is an alphanumeric field: enter a maximum of 12 characters. Press OK to confirm and exit.
STAGE: number of stages to be performed. Depress the text field to enable a virtual keyboard.
This is an alphanumeric field: enter a maximum of 12 characters. Press OK to confirm and exit.
TRIAL: number of trials to be performed. Depress the text field to enable a virtual keyboard.
This is an alphanumeric field: enter a maximum of 12 characters. Press OK to confirm and exit.
ID: Identification number of the animal.
Depress the text field to enable a virtual keyboard.
This is an alphanumeric field: enter a maximum of 12 characters. Press OK to confirm and exit.
In the RESULT table, the entered information will be matched with the test results.
The user may choose not to enter any information and leave the fields empty.
The Home Page icon takes the user back to the home page.

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4 Operation 4 Operation Librae instruction manual > Page: 21
4.3 Setup
Use the Setup page (Figure 4.3.1) to configure the parameters of the experiment:
1. Animal Max Weight: this value allows you to rescale the histogram and the plot chart
during the measurement. Moreover, this value is used during the autostart procedure.
Use a numeric value from 20g to 4400g.
NOTE: if the value is not set correctly, the autostart procedure may not work as ex-
pected. Refer to paragraph 4.6 for more details
NOTE: in manual start mode, the correct value rescales the graph, optimizing the
display of graphical data.
2. Measurement Duration: this value is the maximum duration for the experiment. Numeric
value range is from 1 second to 360 seconds.
NOTE: If necessary, it is possible to STOP the experiment before the elapsed time-out, just
push STOP virtual button (or pedal switch).
3. Start Mode: this sets the operative start mode of Librae. Manual mode allows you to START/
STOP the experiment by pushing the virtual buttons on the screen.
Autostart mode lets Librae automate the start of the experiment..
Refer to paragraph 4.6 for more details.
Figure 4.3.1
4.4 Start
START screen (Figure 4.4.1) enables the starting and stopping of an experiment. Apply ZERO (tare)
to the measurement (if necessary), and see the applied force distribution.
This feature is particularly useful to check the animal position inside the restrainer, and if the
experiment is running correctly.
Figure 4.4.1
Ehorizontal
lements displayed on the START page (from let to right) provide the following functionality:
1. (i) INFO button. This button displays a pop-up windows with experiment details
2. Histogram. This graph displays the weight distribution between Let paw and Right paw.
The weight in grams is also displayed. A horizontal dynamic line allows users to check if
the weight is equally distributed between both paws.
NOTE: the horizontal dynamic line shows the half weight of the animal (this is the expected
weight per paw). This means “how much weight gap” is present between paws.
3. ZERO button. This button applies a “zero balance” to Librae. Use this only if the instrument
is not already in zero position prior to the experiment start.
NOTE: Librae performs a zero balance EVERY TIME the start page is selected. DO NOT APPLY any
force before selecting the start page, APART FROM TARE IF IT IS REQUIRED..
NOTE: Press ZERO button IF a negative weight is shown by histogram or by numeric values.
4. START/STOP button. This contextual button allows users to start or stop the experiment.
Press START to run the experiment. Press STOP to cease it. The trial results window dialog
will be displayed (see figure 4.6.7). Accept or reject the experiment data to proceed.
5. Time Scroll Graph. This graph shows the weight of both paws over time. This allows users
to see the “story” of the signal during the experiment.
NOTE: This graph is for visualization purpose only! Some peaks or spikes may not be visible.
Librae acquires and saves data from the force transducer at maximum speed. Export the .csv file
for data analysis.

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4 Operation 4 Operation Librae instruction manual > Page: 23
4.5 Calibration
Librae Incapacitance Tester is factory calibrated by Ugo Basile technicians. It is possible to
override the original calibration if required by a customer quality policy (or other reasons).
For this purpose, Librae guides the user through a wizard calibration procedure. To run the
procedure, press the CALIBRATION icon on the Home Page Menu (Figure 4.1.1).
NOTE: Before running the calibration wizard procedure, ensure that NO weight is applied on the
food pads, AND a 100g calibrated weight is present. If necessary, Ugo Basile can supply a 100g
calibrated weight. Refer to paragraph 7.0 for ordering details.
Figure 4.5.1 shows the calibration steps procedure. Follow the instructions on the screen to
calibrate your Librae Incapacitance Tester.
Figure 4.5.1
DO NOT REMOVE the weight during the calibration procedure.
Follow the instructions from the calibration wizard.
Ater the execution of all steps, Librae Incapacitance Tester will be calibrated with the new
values.
NOTE: Calibration is not necessary if it is not required by customer quality policy. The
instrument is shipped fully calibrated.
NOTE: During the calibration procedure, Librae MUST be positioned on a stable workbench.
In addition, Librae must be horizontal, level, and stationery. Avoid calibration if this is not
possible..
NOTE: During calibration procedure, the values are stored ONLY ater the confirmation of the
last step. Before that, it is possible to escape the procedure just by pressing CANCEL button and
NO DATA WILL BE STORED.
4.6 Set Autostart
Autostart is a unique Librae Incapacitance Tester feature. By selecting this operative mode,
Librae helps the user to perform the experiment in a consistent way, reducing variability and
user bias or intervention during measurement.
Before using this feature, it is important to set the parameters in order to take advantage of
autostart.
Figure 4.6.1 shows the Autostart settings menu.
1. Autostart Weight Threshold: this parameter sets the minimum weight the Librae MUST
consider for animal. The percentage value from 10% to 90% is referenced to the animal
weight (Refer to paragraph 4.3)
2. Autostart Stabilization Threshold: this parameter sets the percentage weight variation
referenced to the animal weight (Refer to paragraph 4.3). Percentage value from 1% to 20%.
3. Autostart Sustain Time: this parameter sets the acceptable time for satisfying the previous
two parameters. Numeric value from 200 msec to 5 sec.
Figure 4.6.1
To help the user understand how this feature works, an example is shown below.
Let’s assume the test has a mouse that weighs 50 g, the experiment duration is 15 seconds, and
autostart mode is selected.
1. Configure the SETUP menu (refer. to paragraph 4.3) as follows:
1.1 Animal Max Weight: 60g;
1.2 Measurement Duration: 15s;
1.3 Start Mode; Auto.
2. Press the SET AUTOSTART icon, and set the parameters as follows:
2.1 Autostart Weight Threshold: 60%. With this value, Librae considers the presence
of the animal ONLY IF MORE THAN 36g (60% of 60 grams) are measured.
2.2 Autostart Stabilization Threshold: 10%. With this value, Librae considers that the
animal is quiet and ready for experiment ONLY IF the NOISE on foot pads is
LESS THAN 6g (10% of 60 grams). See Figure 4.6.2 for details.
2.3 Autostart Sustain Time: 3s. With this value Librae NEEDS AT LEAST 3S in which the
previous conditions are satisfied.
3. Go to Home Page Menu, and press START button (Figure 4.6.2);

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4 Operation 4 Operation Librae instruction manual > Page: 25
Figure 4.6.2
4. START the experiment (Figure 4.6.3);
Figure 4.6.3 - Autostart is waiting for the minimum weight. AUTO icon is ORANGE.
5. Put the animal inside the restrainer (Figure 4.6.4);
Figure 4.6.4 - Autostart is waiting for the weight stabilization. AUTO icon is YELLOW.
When ALL the autostart requirements are completed, the experiment automatically starts
(Figure 4.6.5).
Figure 4.6.5- Autostart is waiting for stabilization time to elapse. AUTO icon is GREEN.
6. Experiment runs until time-out (Figure 4.6.6)
Figure 4.6.6 - Experiment is running
When the experiment is finished, a trial results windows is displayed, as Figure 4.6.7 shows. The
experiment results can be accepted or rejected. A new experiment can be started.
Figure 4.6.7
NOTE: both accepted and rejected experiments are stored into the experiment db records.

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4 Operation 4 Operation Librae instruction manual > Page: 27
4.7 Utilities
UTILITIES menu (Figure 4.7.1) contains six submenus for setting the instrument, exporting data,
checking results, and more.
Figure 4.7.1
4.7.1 Update
This function allows you to update the sotware of the instrument. Before running the update
procedure, check the installed firmware version (see Figure 4.1.3) and check Ugo Basile website
for the latest version, (www.ugobasile.com)
To perform a sotware update:
FIRSTLY SAVE YOUR DATA AND EXPERIMENT BEFORE PROCEEDING!
Next:
1. Download the sotware update from Ugo Basile website
2. Copy the update file into a USB memory key.
3. Insert the USB memory KEY into Librae Incapacitance tester.
4. Wait for update system icon (refer. to paragraph 4.1.1)
5. Press Update button and wait for a reboot.
Figure 4.7.1.1
Now, the Librae Incapacitance Tester is up to date. An Erase DB could be necessary. Refer to
paragraph 4.7.4 for details.
NOTE: DO NOT REMOVE USB memory key during update. The instrument will be damaged.
NOTE: Insert the USB memory key before update procedure. If not, Librae will show a warning
message, WITHOUT performing update
NOTE: DO NOT MODIFY the update binary file.
4.7.2 Date-Time
Date and TIme menu allows to change the calendar and clock settings, as shown in FIgure 4.7.2.1.
Use the up/down arrows to adjust current time and date. Press OK to confirm
Figure 4.7.2.1
4.7.3 Advanced Settings
Advanced setting allows users to change the weight averaging parameter (Figure 4.7.3.1). Numeric
values range from 1 sample to 10 samples. This parameter aects ONLY the visualization of the
graph during the experiment.
NO DOWNSAMPLING OR DECIMATION WILL BE APPLIED TO MEASUREMENTS.
Figure 4.7.3.1

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4 Operation 4 Operation Librae instruction manual > Page: 29
4.7.4 Erase DB
Erase DataBase button allows the deletion of ALL data and experiments stored on the Librae
Database. This funcion is normally NOT required.
Ater the button is pressed, a Confirm window appears. Press OK to delete the database. Press
CANCEL to escape.
NOTE: ONLY Use this command ater a sotware update procedure. Refer to paragraph 4.7.1 for
details.
WARNING: ALL YOUR DATA WILL BE PERMANENTLY DELETED!
4.7.5 Results
Results button shows (Figure 4.7.5.1) the results of the experiments stored inside Librae
Incapacitance Tester.
Every result box contains:
1. Date and time of the experiment
2. Treatment, ID, protocol, stage and trial
3. Experiment accepted or rejected
4. Duration
5. Functional mode
6. Peak and average values for each paw
Use the up/down arrows to scroll through results.
Figure 4.7.5.1
NOTE: The result box displays only a small set of data. Download the .csv file for the complete
experiment data.
4.7.6 USB Storage
USB Storage menu (Figure 4.7.6.1) allows to save experimental data to USB memory key,
Figure 4.7.6.1
Data will be exported in .csv format. Use a text editor, spreadsheet or Microsot Excel to open
and analyze your experiment data.
NOTE: INSERT a USB memory key BEFORE SAVING or LOADING any data.

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4 Operation 4 Operation Librae instruction manual > Page: 31
4.8 Connections
4.8.1 Communication port connection
Librae Incapacitance Tester is provided with a D-sub (DA-15 Female) TTL I/O port (see Figure
3.8.2). This port can be used to synchronize some events with external instruments or
acquisition systems. TTL Output signal are electrically isolated to provide an electrical barrier
between Librae Incapacitance Tester and any other external device.
TTL signals are refered to Power Ground (pimn 14 and pin 15)
Refers to Figure 4.8.1.1 and Table 4.8.1.1 for connector pinout
Figure 4.8.1.1
DB-15 Pin# Signal Name Signal Type Description
1 Start/Stop TTL OUT Start -> TTL High Level
Stop -> TTL Low Level
2 Manual/Autostart TTL OUT Manual -> TTL High Level
Autostart -> TTL Low Level
3 Reserved TTL OUT Reserved
4 Reserved TTL OUT Reserved
5 Reserved TTL OUT Reserved
6 Reserved TTL OUT Reserved
7 Reserved TTL OUT Reserved
8 Reserved ANALOG OUT Reserved
9 Reserved TTL IN Reserved
10 Reserved TTL IN Reserved
11 Reserved TTL IN Reserved
12 Reserved TTL OUT Reserved
13 Reserved TTL OUT Reserved
14 GND POWER Power Ground
15 GND POWER Power Ground
Table 4.8.1.1
NOTE: TTL OUT is designed for connection with scientific instruments! DO NOT CONNECT ANY
POWER DEVICE!
NOTE: DO NOT SINK a current more than 10mA from each TTL pin. DAMAGE WILL OCCUR

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5 Maintenance 6 Specifications Librae instruction manual > Page: 33
5 Maintenance
While any service or repairs of the instrument are performed by Ugo Basile, this section
describes normal maintenance procedures that can be performed at your facility.
UNPLUG THE POWER CORD BEFORE CARRYING OUT ANY MAINTENANCE WORK
5.1 Electrical
To inspect and/or replace the fuses, disconnect the mains cable first! Insert a miniature screw-
driver in the slot indentation and snap out the slide which houses the fuses. Use T400mA fuses
for operation at both 230 or 115 Volts.
Snap in the fuse slide: the mechanical “click” ensures that it is locked.
5.2 Cleaning/disinfection
The Librae unit (47880) does not require any maintenance apart from normal cleaning.
Do not use organic solutions on the restrainer and cabinet, as they are liable to impair the
transparency of the restrainer and crack the acrylic component and the touch pad.
Cotton wool and water can be used for cleaning purposes. To disinfect, use a non-alcoholic
disinfectant, or H2O.
5.3 Long Inactivity
The instrument does not require any particular maintenance ater long inactivity, except
cleaning
5.4 Customer Support
For any further information you may require concerning the use and/or maintenance of the
Librae Incapacitance Tester, please do not hesitate to contact our service department (or our
local distributor) either directly of via our support page. Before sending any instrument to
our factory for repair, please contact our logistics department to obtain a return authorization
number (RMA) and shipping/packing instructions. We may not be held responsible for damages
caused during transport due to poor packing. Whenever possible, please use the original
packing.
6 Specifications
General
Command Input 4.3’’ touch-screen (resistive)
Read-out 4.3’’ TFT touch-screen
Power Requirements Universal input 85-264 VAC, 50-60Hz, 15W max.
Sound Level < 45dB(A)
Operating Environment 10°C to 40°C; 5% to 95% RH (non-condensing)
Operation
Animal weight (per paw) from 20g to 4400g
Precision from 0.05% to 0.1%
Measurement Duration from 1s to 360s
Measurement Mode Manual and Autostart
Autostart parameters Weight threshold, Stabilization Threshold,
Sustain Time
Weight Averaging 1 sample to 10 samples
Start Experiment Start button, pedal switch, or Autostart
Stop Experiment Stop button, pedal switch, or elapsed
timeout
Data export .csv format, from USB key (provided)
TTL Output Start/Stop, Measurement Mode.
5VDC +/- 10%; 10mA MAX
Physical
Total Weight 2.5kg
Shipping Weight 5kg
Dimensions (without restrainer) 25cm(w) x 31cm(d) x 13cm(h)
Dimensions (including restrainer) 25cm(w) x 31cm(d) x 25cm(h)
Packaging dimensions 68cm(w) x 34cm(d) x 28cm(h)
Warranty
Product Warranty The Librae Incapacitance Tester unit (47880) is
covered by a 12-month warranty. Coverage can
be increased to 24-months for free when you
register your instrument on the UGO BASILE
website.

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Other Ugo Basile products related to this device
visit ugobasile.com web site for details
Ugo Basile SRL
Via Giuseppe Di Vittorio 2, 21036 Gemonio (VA) ITALY - Tel. [phone] - [email protected]om
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Plethysmometer, the 1st and original device for
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(Hargreaves Apparatus)
Thermal Place Preference (TPP Test) for Mice &
Rats
Analgesy-Meter the 1st and original Randall-Selitto
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Other Ugo Basile products related to this device
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Via Giuseppe Di Vittorio 2, 21036 Gemonio (VA) ITALY - Tel. [phone] - [email protected]om
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I.R. Heat-Flux Radiometer for Tail Flick and
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Additional Ugo Basile products for motory coordination
Visit ugobasile.com web site for details
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Via Giuseppe Di Vittorio 2, 21036 Gemonio (VA) ITALY - Tel. [phone] - sales@ugobasile.
com
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