VisualSonics VEVO User manual

VisualSonics
VevoTM Compact Anesthesia System
Operator Manual

V1.0 VisualSonics Vevo Compact Anesthesia System Operator Manual i
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VisualSonics
Vevo Compact Anesthesia System
Operator Manual
November 2003
VS-OM-AS revision 1.0

ii VisualSonics Vevo Compact Anesthesia System Operator Manual V1.0
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VisualSonics Inc.
3080 Yonge Street, Suite 6020
Box 89
Toronto, Ontario
CANADA
M4N 3N1
Tel: [phone]
Toll Free: 1-866-416-4636
Fax: [phone]
www.visualsonics.com
Copyright © 2003 by VisualSonics Inc.
All Rights Reserved
Printed in Canada
VisualSonics, the VisualSonics logo, Vevo, and Vevo Compact Anesthesia
System are trademarks of VisualSonics Inc.
All other trademarks are the property of their respective owners.

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Safety
Please read the safety information before using the Vevo Compact
Anesthesia System. The following information applies to the Vevo
Compact Anesthesia System and its modules.
The use of this equipment must be directed by, is intended for use by, and
under the supervision of qualified research scientists.
Read all warnings and cautions carefully before use!
WARNINGS
• For small animal use only. Not for human use.
• Do not fill the vaporizer with any anesthetic agent other than the one
specified on the front label. The vaporizer is designed for that agent
only. Any other agent than that specified can prove to be dangerous
to a subject.
• If a vaporizer is filled with the wrong agent, draining will not
eliminate the agent since some of the agent will have been absorbed
into the wick. The wick must be thoroughly cleaned and dried. This
procedure must be performed at an authorized service center.
• Do not carry the vaporizer by the control dial.
• Do not modify, tamper with, or disassemble the vaporizer. There is a
potential danger of damaging the vaporizer and altering the
calibration accuracy.
• Do not immerse the vaporizer in any liquid, including water.
• Do not sterilize the vaporizer.
• Do not drain the anesthetic agent into any container other than a
properly marked container.
• Incomplete sealing of the fill cap will result in loss of gases.
• Do not fill the vaporizer unless the control dial is in the ‘Off’ position.
• Do not turn the dial ‘On’ during filling or attempt to fill beyond the
‘Full’ mark.
Warnings are precautions necessary to prevent injury
or loss of life.
Cautions are precautions necessary to protect the equipment.

iv VisualSonics Vevo Compact Anesthesia System Operator Manual V1.0
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• Keep the vaporizer upright at all times.
• Never tilt a calibrated vaporizer beyond 45 degrees when charged.
Dangerous overdose may result during subsequent use. This
condition can be detected by calibration verification only.
• Do not connect the vaporizer directly into the patient breathing
circuit.
• Do not turn on two vaporizers at the same time.
• Do not put water or any other solvent in a vaporizer.
• Due to the wick’s capacity to absorb agent, the vaporizer will
continue to deliver the set concentration for a considerable period of
time even if no liquid level can be viewed through the sight window.
However, do not use a vaporizer in this condition on a subject.
• Flush flows through an open vaporizer may result in unwanted
concentrations of anesthetic agent.
• Do not ignore maintenance of your vaporizer. An annual calibration
recertification is highly recommended by the manufacturer.
CAUTIONS
• Personnel operating this compact anesthesia system must be
thoroughly familiar with the instruction manual and equipment
operation prior to use with subjects.
• Tampering with the anesthesia system components by unauthorized
personnel voids all warranties and specifications. The prevention of
tampering with the anesthesia system is the sole responsibility of the
user or owner. VisualSonics assumes no liability for any malfunction,
failure, damage or loss to either equipment or life.
• Before using the anesthesia system check that it is set up properly,
ensure all connections and settings are correct.
• Always adhere to proper Diameter Index Safety System (D.I.S.S.)
hose connections. Failure to comply will void your warranty and
could compromise subject safety.
• Remove the machine from service if:
a. the oxygen flush button does not immediately return to the OFF
position after being released, or
b. the flow of oxygen does not immediately stop flowing from the
common outlet after the oxygen flush button is released.
Then call VisualSonics or a VisualSonics-authorized service
facility to repair the valve.
• If the oxygen flush button does not immediately return to the off
position after being released, or if the flow of oxygen does not
immediately stop flowing from the common outlet after the oxygen

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flush button is released, remove the machine from service. Have the
valve repaired by VisualSonics or a VisualSonics-authorized service
facility before using the machine.
• When you turn off the flowmeter, turn the control knob clockwise
just far enough so the ball float reaches the bottom of the tube.
Continuing to tighten the control knob will damage the valve seat
and cause a leak. The unit will need to be replaced when this damage
occurs.
• Vaporizer should be serviced by an authorized service center.
• Turn the vaporizer ‘Off’ when not in use.
• A vaporizer MUST be in a vertical upright position to indicate liquid
agent levels accurately.
• If a screw-cap filler vaporizer is pressurized, turn the screw slowly
before filling.
• The delivered concentration may be different from the set
concentration if the vaporizer is operated in a tilted position.
• Avoid kinks in the tubing that will restrict flow. Eliminate leaks that
will affect total flow to the subject.
• To fill the vaporizer when in use, it will be necessary to turn the
vaporizer off.
• No repair should ever be undertaken or attempted by anyone not
having the training and qualifications to perform these tasks.
Liability notice
Failure to follow the conditions set forth below shall limit, to the extent
allowed by law, VisualSonics Inc’s responsibility for the safety, reliability,
and performance of this equipment:
• Personnel operating this compact anesthesia system must be
thoroughly familiar with the operator manual and equipment
operation prior to use with subjects.
• Tampering with the anesthesia system components by unauthorized
personnel voids all warranties and specifications. The prevention of
tampering with the anesthesia system is the sole responsibility of the
user or owner. VisualSonics assumes no liability for any malfunction,
failure, damage or loss to either equipment or life.
• Always adhere to proper Diameter Index Safety System (D.I.S.S.)
hose connections. Failure to comply will void your warranty and
could compromise subject safety.
• This equipment must be periodically checked, calibrated, maintained
and, components repaired and replaced when necessary for
equipment to operate reliably. Parts that have failed in whole or in
part, exhibit excessive wear, are contaminated or are otherwise unfit
for use, should be immediately discarded and replaced. To maintain

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the warranty, replacement parts must be installed or approved by
VisualSonics Inc. Equipment that is not functioning correctly must
not be used. This equipment must not be modified by unauthorized
personnel or with unauthorized components.

:
V1.0 VisualSonics Vevo Compact Anesthesia System Operator Manual vii
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Contents
Introduction
Welcome .................................................................................................. 1
Product description................................................................................ 1
Table-top and mobile configurations .................................................. 1
Chapter 1: Unpacking the System
Unpacking the table-top configuration............................................... 3
Unpacking the mobile configuration................................................... 5
Chapter 2: Installing the System
Choosing a location for the system...................................................... 9
Installation overview ............................................................................. 9
Assembling the delivery sub-system................................................... 9
Connecting the delivery sub-system to the vaporizer sub-system10
Connecting the oxygen supply to the vaporizer sub-system......... 11
Troubleshooting.................................................................................... 14
Chapter 3: Theory of Operation
System capability.................................................................................. 15
Theory of operation.............................................................................. 15
Chapter 4: Operating the Vaporizer
Principle of operation .......................................................................... 17
Turning on the vaporizer .................................................................... 18
Filling the vaporizer............................................................................. 19
Draining the vaporizer ........................................................................ 20
Reminders for filling and draining.................................................... 21
Chapter 5: Anesthetizing a Mouse
Pre-operative checks ............................................................................ 23
Inducing anesthesia ............................................................................. 23

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Chapter 6: Service
Maintenance .......................................................................................... 27
VisualSonics support ........................................................................... 28
Appendix A: Accessories
Accessories for the compact anesthesia system............................... 29
Appendix B: Specifications and Performance
Specifications......................................................................................... 31
Performance effects of variables......................................................... 32
Index....................................................................................................... 35

Introduction
V1.0 VisualSonics Vevo Integrated Rail System Operator Manual 1
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Introduction
Welcome Thank you for choosing the Vevo™ Compact Anesthesia System, the
Visualsonics’ anesthesia solution for small animals, such as mice.
Product description The Vevo Compact Anesthesia System integrates two sub-systems: a
vaporizer sub-system and a delivery sub-system.
Vaporizer sub-system The vaporizer sub- system mixes oxygen and anesthesia into a vapor. It
features a stand assembly with oxygen flowmeter, oxygen flush valve,
precision vaporizer and a 10-foot oxygen hose assembly with threaded
DISS (Diameter Index Safety System) connections.
Delivery sub-system The delivery system supplies the metabolic and anesthetic gas mixture to
the mouse. It features a dual breathing circuit that includes a 2-liter
induction chamber breathing circuit, a mouse mask breathing circuit, and
two activated charcoal filters for waste gas removal.
The system is also designed to function as a companion to the Vevo
Integrated Rail System and the Vevo 660™ High Resolution System to
provide an efficient and complete mouse imaging workspace.
Table-top and mobile
configurations
The Vevo Compact Anesthesia System is offered in two system
configurations: a table-top configuration and a mobile configuration.
Table-top vaporizer sub-
system configuration
The compact and versatile table-top sub-system features a tiny footprint
of 8” x 11”, making it easy to integrate into existing lab counter space. The
sub-system includes weight-balanced handles and non-skid feet so you
can set it in place anywhere - quickly, safely and securely.
Oxygen
flowmeter
Flow
control
knob
Vaporizer
dial
Vaporizer
Oxygen flush
Common
outlet
Carrying
handles

Introduction
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Mobile vaporizer sub-
system configuration
The mobile system features sturdy casters, a built-in handle so you can
move it easily to another room or another building, a convenient syringe
holder, and a stainless steel shelf that gives you a handy workspace. All
internal connections are housed in a smooth panel so you can clean the
system easily.
Common delivery sub-
system
The delivery sub-system is a single configuration that functions
identically with each vaporizer sub-system configuration.
Oxygen
flowmeter
Flow
control
knob
Vaporizer
dial
Vaporizer
Oxygen
flush
Common
outlet
Handle/
hanger
Syringe
holder
Stainless steel
shelf
Y-tube
Induction chamber
Charcoal filter
Mouse mask
Stopcocks
Anesthesia - in
Mouse mask
(waste gas - out)
Induction chamber
(waste gas - out)

Chapter 1: Unpacking the System: Unpacking the table-top configuration
V1.0 VisualSonics Vevo Compact Anesthesia System Operator Manual 3
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Chapter 1: Unpacking the System
Unpacking the table-
top configuration
If you ordered the table-top configuration of the compact anesthesia
system (Part # SA-TABLEANES), the crate will include the items listed
below. Please use the forms below to confirm that you have received the
included items.
Pre-assembled vaporizer
sub-system
The vaporizer sub-system is pre-assembled as shown below.
The vaporizer sub-system for the table-top configuration includes the
following items:
Oxygen
flowmeter
Oxygen
flush
Common
outlet
Isoflurane
Vaporizer
with pin-
index
Check Quantity/unit Description
1 each Oxygen flowmeter (see photo above)
1 each Oxygen flush (see photo above)
1 each Isoflurane vaporizer with pin-index (see
photo above)
1 each Inlet and outlet (pre assembled on the
vaporizer)

Chapter 1: Unpacking the System: Unpacking the table-top configuration
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Unassembled delivery sub-
system for the table-top
configuration
The delivery sub-system is unassembled and includes the following
items:
1 each Pin-index fill device
1 each Green 10’ oxygen hose DISS x DISS
Check Quantity/unit Description
1 each Y-tube with 15x6mm adapter, 9mm mouse
mask with 12” wastegas flexhose for
mousemask, 48” silicone tubing from
stopcock to adapter, and 30” of 22mm semi-
transparent tubing
1 each 2-liter induction chamber
2 each Activated charcoal canister

Chapter 1: Unpacking the System: Unpacking the mobile configuration
V1.0 VisualSonics Vevo Compact Anesthesia System Operator Manual 5
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Unpacking the
mobile configuration
If you ordered the mobile configuration of the compact anesthesia system
(Part # SA-MOBILEANES), the crate will include the items listed below:
Pre-assembled vaporizer
sub-system
The vaporizer sub-system is pre-assembled as shown below:
The vaporizer sub-system for the mobile configuration includes:
1 each 22mm tubing (2-feet length)
1 each Monitoring badge
Check Quantity/unit Description
Oxygen
flowmeter
Oxygen
flush
Common
outlet
Handle /
hanger
Front view Rear view
Oxygen
inlet
Isoflurane
Vaporizer
with pin-
index
Syringe
holder
Check Quantity/unit Description
1 each Oxygen flowmeter (see photo above)
1 each Oxygen flush (see photo above)
1 each Isoflurane vaporizer with pin-index (see
photo above)

Chapter 1: Unpacking the System: Unpacking the mobile configuration
6 VisualSonics Vevo Compact Anesthesia System Operator Manual V1.0
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Unassembled cart legs The cart legs are unassembled and include the items listed below.
Assembly instructions for the cart legs are included with the cart leg
parts. Please follow the instructions to complete the assembly.
1 each Syringe holder (see photo above)
1 each Handle/hanger (see photo above)
1 each Inlet and Outlet (pre assembled on the
vaporizer)
1 each Pin-index fill device
1 each Green 10’ oxygen hose DISS x DISS
1 each Stainless steel shelf with mounting
screws and washers
Check Quantity/unit Description
Check Quantity/unit Description
1 each 2 cart legs

Chapter 1: Unpacking the System: Unpacking the mobile configuration
V1.0 VisualSonics Vevo Compact Anesthesia System Operator Manual 7
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Unassembled delivery sub-
system for the mobile
configuration
The delivery sub-system is unassembled and includes the following
items:
Check Quantity/unit Description
1 each Y-tube with 15x6mm adapter, 9mm mouse
mask with 12” wastegas flexhose for
mousemask, 48” silicone tubing from
stopcock to adapter, and 30” of 22mm semi-
transparent tubing
1 each 2-liter Induction Chamber
2 each Activated Charcoal Canister
1 Holder for an activated charcoal canister

Chapter 1: Unpacking the System: Unpacking the mobile configuration
8 VisualSonics Vevo Compact Anesthesia System Operator Manual V1.0
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1 each 22mm Tubing (2-feet length)
1 each Monitoring Badge
Check Quantity/unit Description

Chapter 2: Installing the System: Choosing a location for the system
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Chapter 2: Installing the System
Choosing a location
for the system
The Vevo Compact Anesthesia System must be situated in an
environment that ensures maximum safety for the operator and for
others working in the building. Consider these guidelines as you
determine the location for the system.
Ventilation and air flow Ensure adequate air flow and ventilation to prevent anesthesia from
being inhaled by anyone other than the intended subjects. This is a vital
requirement. Check with your jurisdiction’s occupational health and
safety authorities to determine required levels of air flow and ventilation.
Oxygen tank location Oxygen tanks should be adequately restrained to prevent damage to the
regulators, especially due to the highly pressurized nature of the
cylinders. Custom carriers are available for E-cylinders. H-cylinders
should be chained in place.
Installation overview When you have unpacked the items (and assembled the cart legs if you
ordered the mobile configuration), the installation procedure follows the
same three steps for each configuration:
1. Assemble the delivery sub-system.
2. Connect the delivery sub-system to the vaporizer sub-system.
3. Connect the oxygen supply to the vaporizer sub-system.
Assembling the
delivery sub-system
The delivery sub-system manages the delivery and evacuation of
anesthesia to the two breathing circuits. Assembling the delivery sub-
system takes approximately five to ten minutes to complete. A completed
delivery sub-system is shown below.
Y-tube
Induction chamber
Charcoal filter
Mouse mask
Stopcocks
Anesthesia - in
Mouse mask
(waste gas - out)
Induction chamber
(waste gas - out)

Chapter 2: Installing the System: Connecting the delivery sub-system to the vaporizer sub-system
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To assemble the induction chamber breathing circuit:
1. Insert one branch of the Y-tube into the
induction chamber port you have chosen to
receive the anesthesia. It doesn’t matter which
port you choose, left or right. Likely you will
want to choose the port that is closest to the
vaporizer system.
2. Insert a length of semi-transparent
flexhose over the second induction chamber
port. This will serve as the waste gas
evacuation port.
3. Insert one of the charcoal filters into the
flexhose.
To assemble the mouse mask breathing circuit
1. Insert one branch of the Y-tube into the 15mm (larger) end of the
15x6mm adapter.
2. Insert the anesthesia line of the mouse mask into the 6mm (smaller)
end of the 15x6mm adapter.
3. Insert the blue flexhose of the mouse mask into the 19mm (smaller)
end of the 19x22mm adapter.
4. Insert the second charcoal filter into the 22mm (larger) end of the
19x22mm adapter.
The delivery sub-system is now assembled.
Connecting the
delivery sub-system
to the vaporizer sub-
system
The vaporizer sub-system arrives fully assembled. Now that you have
assembled the delivery sub-system, you need to connect the two sub-
systems.
The connection procedure takes about five seconds.
To connect the delivery sub-system to the vaporizer sub-system:
1. Insert the Y-tube’s blue connector into the vaporizer sub-system’s
common outlet. This is a friction fit connection.
Use as short a length of
f
lexhose as is convenient. If
you need to cut the supplied
f
lexhose to length, cut in the
centre of the connection area,
as shown above, to ensure a
proper connection fit.
In the table-top configuration, the common outlet is located at the tip of the delivery tube.