Olympus IX71 User manual

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Photos courtesy of:
Atsushi Miyawaki M.D., Ph.D, Ms. Asako Sakagami, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics (P8)
Teruhiko Wakayama, Ph.D., Laboratory for genomic Reprogramming, Head of Laboratory, Riken Kobe Institute, Center for Developmental Biology (CDB) (P13)
Yuji Abe M.D.Ph.D., The 1st Department of Obstetrics & Gynecology School of Medicine, Toho University (P21)
Atsushi Miyawaki M.D., Ph.D, Ms. Ryoko Ando, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics (P22, P26)
Atsushi Miyawaki M.D., Ph.D, Mr. Hideaki Mizuno, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics (P25)
Tohru Murakami, M.D., Ph.D, Department of Neuromuscular and Developmental Anatomy, Gunma University Graduate School of Medicine (P23 above, zebra fish)
Dr. Takeshi Awasaki and Dr. Kei Ito, Institute of Molecular and Cellular Biosciences, The University of Tokyo (P23 below, drosophia)
Dr. Kazuo Kurokawa, Department of tumor virology, Research institute for microbial diseases, Osaka university (P24 below, Kaede-Crk II protein expressed in a HeLa cell)
Research
Inverted System Microscope
IX71/IX81
IX2 Series
Built for Live Cell Imaging
Printed in Japan M1539E-0610B

As new fluorochromes are developed and new methods of light excitation and manipulation become more popular
for live cell experiments, more and more researchers will require the use of low phototoxicity near-IR wavelengths in
addition to the conventional visible spectrum. Olympus has equipped its IX2 series microscopes with the new UIS2
optical system precisely to meet those demands. With high S/N ratio, compensation for chromatic aberration over a
much wider wavelength range and flat, high transmittance , this new system sets a new world standard of
fluorescence performance — efficiently detecting even faint fluorescence signals without damaging the cell,
optimizing multi-color observation. Delivering unprecedented image quality over a super wide light spectrum,
the IX2 inverted system microscope will be your choice of live cell imaging now and in the future. IX71
Olympus IX2 inverted microscopes
combined with the new UIS2 optical system
opens a new world of live cell imaging.
IX81/IX81-ZDC
Motorized inverted system microscope
IX81/IX81-ZDC
Motorized System
Research inverted system microscope
IX71
Manual System
21

3
Superior S/N ratio delivers imaging excellence
UIS2 objectives' fluorescence S/N ratio is improved by using
selected low fluorescence glass material, and minimized
autofluorescence obtained by anti-reflection coating and cementing
material. Also numerical aperture (N.A.) has improved in addition to
the reduction of autofluorescence. Weak fluorescence emissions
are efficiently detected even from weak excitation light, which is
friendly for the living cell.
The ideal fluorescence imaging of live cells with the UIS2 systems!
High transmittance UPLSAPO100XO
Transmittance(%)
Wavelength (nm)
0
300 400 500 600 700 800
10
20
30
40
50
60
70
80
90
100
(new) UPLSAPO100XO
(conventional) PLAPO100XO
Focus (µm)
Wavelength (nm)
Comparing chromatic aberration compensation levels:
(The smaller the figure the better)
UPLSAPO series chromatic aberration compensation
-0.5
0
400 450 500 550 600 650 700 750 800 850 900
0.5
1
1.5
2
(new) UPLSAPO100XO
(conventional) UPLAPO100XO
Alexa Fluor488 Cy7
UIS2 optics are designed to maximize S/N ratio and optical
performance for live cell fluorescence imaging.
High transmittances over a wider
wavelength range
UIS2 objective for the IX71/81 achieves flat, high transmittance from
visible to near-infrared light, thanks to new UW multi-coating which
effectively cuts reflection over the super wide band spectrum. In
particular, transmission in the near infrared range is significantly
enhanced. Overall, performance all across the wavelength range is
ideally suited for today's most demanding research applications.
High N.A. objectives for fluorescence imaging
PLANAPO60XO and UPLSAPO100XO have outstanding N.A., 1.42
and 1.4 respectively, suitable for fluorescence imaging and bright-
field observation as well. In addition to their high fluorescence S/N
ratio, both these lenses are able to handle UV excitation light. The
UPLSAPO100XO provides a transmission of down to 340 nm.
Aspherical collector lens provides
excellent excitation efficiency.
Unique, optional ring slit
illumination reduces
objective autofluorescence.
Objectives providing
the best fluorescence
S/N ratios.
High performance mirror units
optimized for fluorescence.
Stray light reduction to
absorb spurious reflections
from dichromatic mirror.
4
Effective compensation for
chromatic aberration up to near-infrared
The UPLSAPO series is the highest class UIS2 objectives, whose
super apochromatic features effectively compensate for chromatic
aberration from the visible spectrum all the way to near IR. This
means that just one objective covers imaging from UV to IR range.
The series also offers outstanding image clarity without color shift
for multi-color observations using fluorochromes covering a wide
wavelength spectrum.
UPLSAPO100XO
Cy7
Wavelength:
767 nm
Alexa
Fluor488
Wavelength:
519 nm
UPLAPO100XO
Objective:
UPLSAPO100XO
Mirror unit:
U-MNIBA3
Objective:
UPLAPO100XO
Mirror unit:
U-MNIBA2

Tier-1 Tier-2 Bottom
65
Two-tier multi-port design ensures input/output flexibility.
■IX2 Two-tier optical path
Optical path as seen from
the left side of
the microscope
Objective
Optical path as seen from
the front of
the microscope
Mirror unit
Tube lens
Right side port
Left side port
Lower back port
Upper Tier Lightpath Selection (optional)
Located between objective and tube lenses so a parallel
pencil of rays can be obtained or introduced. Primary
image can be gained by adding a tube lens. Inserting
optical components such as a dichromatic mirror does
not produce a double image. (The alternative of the right
side port)
Lower Tier Lightpath Selection (included)
Located below the tube lens inside the frame, this tier
allows primary image access to either the left side port
or lower back port.
Bottom Lightpath Selection
A direct primary image can be obtained without any
reflections and any optical components.
Tier-1:
Upper optical path
selection
Tier-2:
Lower optical path
selection
Bottom optical
path selection
■Left side port
This port offers a high quality
primary image, located in 102 mm
distance from the microscope
frame for maximum flexibility in
mounting filter wheels or any kinds
of camera adapters.
■Dual port camera adapter /
U-DPCAD* (C-mount,
left side port)
This unique dual port adapter
enables the provision of two
primary images suitable for live cell
imaging.
* optional unit
The input/output of a parallel pencil of rays and the multiple port
structure for gaining the primary image are designed internally in the
form of tiers. To maximize the possible wavelength width, the
optical path branching of each tier is also compatible with the near-
infrared spectrum. Even when more than one port is being used
simultaneously, there is no change in the stage height; as a result,
rigidity and illumination performance remain constant.
Two-tier optical design is also near-IR compatible
Optical Port system
102 mm from left side port
mounting position to primary image.
■Lower back port
■Left side port
■Bottom port
■Right side port
■Binocular port
102 mm
■Lower back port
Enables the attachment of a
C mount camera. Only 1X
magnification is available.
■Bottom port /
IX2-TVR (T-mount)
Primary image access is also
available at the microscope bottom.
IR compatible
IR compatible
IR compatible
IX2-SIDEPORT 現行vsエコ化AWマルチ 計算値 obなし
0
10
20
30
40
50
60
70
80
90
100
300 400 500 600 700 800 900 1000
波長(nm)
透過率%
IX2-SIDE現行-A IX2-SIDEエコ化（KTL-ECO）-AW
0
10
20
30
40
50
60
70
80
90
100
300 400 500 600 700 800 900 1000
Wavelength (nm)
Transmittance (%)
Current IX2 left side port New IX2 left side port
IX2 Side port: Transmittance improved by new coating
With the introduction of the new UIS2 optical system, the IX2 series
offers improved IR transmittance for the side port, back port and
bottom port, providing versatile, high-performance response to
future research demands.
Improved near-infrared transmission
■Right side port / IX2-RSPC-2
The right side port unit (IX2-RSPC-2:
option, F.N.: 16) comes with a tube
lens and accepts a C-mount CCD
camera.

qOlympus offers a range of other high numerical aperture
objectives whose reduced autofluorescence and specially selected
glass contribute to improved fluorescence S/N ratios.
Especially the PLAPON60XO has outstanding N.A., which is 1.42.
N.A. W.D. (mm)
UPLSAPO 10X2 0.40 3.1 mm
UPLSAPO 20X 0.75 0.6 mm
UPLSAPO 40X2 0.95 0.18 mm
UPLSAPO 60XO 1.35 0.15 mm
UPLSAPO 100XO 1.40 0.13 mm
PLAPON60 XO 1.42 0.15 mm
UPLFLN40XO 1.30 0.2 mm
LUCPLFLN 20X 0.45 6.6 — 7.8 mm
LUCPLFLN 40X 0.60 2.7 — 4 mm
LUCPLFLN 60X 0.70 1.5 — 2.2 mm
q
87
Improved S/N ratio enables efficient detection of
even weak fluorescence.
Better S/N ratio delivers brighter, higher-contrast
images in fluorescence observation.
The ideal microscope allows bright, high contrast fluorescence
observation from the minimum amount of excitation light in order to
minimize cell damage or fluorescence fading. To detect a weak
fluorescence signal (S) efficiently, all other light noise (N) must be
reduced. Therefore, it is very important for fluorescence observation
to maximize the signal (S) and to minimize the noise (N).
Measures to enhance the signal (S)
qFluorescence objectives with high N.A.
wFilters matched to the wavelength
characteristics of individual
fluorochromes
Measures to reduce noise (N)
qObjectives without autofluorescence
wNo crossover between excitation &
emission light with new introduced
filters.
eOptical system that prevents entry of
stray light
rRing slit illumination to reduce
autofluorescence
Fluorescence Observation Units
wThe S/N ratio of certain interference type fluorescence mirror
units is now improved, thanks to the application of new coating
technology to narrow the gap between excitation (Ex) and emission
(Em). The line-up has been extended for wide variety of choice.
U-MNIBA3 U-MGFPHQ
400 450 500 550 600 650
0
10
20
30
40
50
60
70
80
90
100
Transmittance (%)
Wavelength (nm)
BP470-495
DM505
BA510-550
Transmittance (%)
400 450 500 550 600 650
0
10
20
30
40
50
60
70
80
90
100
Wavelength (nm)
DM485
BP460-480
Ex
BA495-540
Em
High S/N ratio objective with reduced autofluorescence
High-performance fluorescence mirror units for fluorescent proteins
Improved performance of interference type fluorescence mirror unit
High performance mirror units
FL SN
SN
S
N
SN
FL
w
w
e
r
e The slight transmission of stray light when excitation light is
reflected in the dichromatic mirror causes a rise in the level of noise.
Olympus mirror units absorb more than 99% of this stray light
through their light absorber.
Light
source Stray light
Excitation filter
Emission filter
Stray light
reducing
function
Fluorescence light
for observation
Dichromatic
mirror
Objectives
Specimen
Excitation light:
Illumination light
●A multi-band dichromatic mirror is normally used to obtain multi-
color images of multiple stained fluorescent samples by using filter
wheels on the excitation and emission sides. However, this kind of
mirror encounters the problem that each fluorescence image gets
darker as the number of color dyes increase, because the
transmission spectrum becomes narrower and the transmittance
falls to lower than 90% at best. Olympus has therefore developed
the world’s first glass reflector that is not wavelength-dependent,
offering a high transmittance of 94% across a wide wavelength
range from 430 nm to 700 nm. Used in combination with the filter
wheels on the excitation and emission sides, a wider variety of color
dyes can be used and
fluorescence images are captured
more efficiently. *Special order basis product
■Glass reflector specifications
26 X 38 mm (t=1 mm) glass substrate
Transmittance 94% (at 430-700 nm)
26 X 38 mm
* Observations through eyepieces may have
some restrictions
Sapphire-pm. CFP-CaM
YFP-mt DsRed-nu
Simultaneous imaging of Sapphire, CFP, YFP, and RFP. HeLa cells were imaged for
Sapphire-pm, CFP-CaM, YFP-mt, and DsRed-nu. The images were obtained using the glass
reflector in a normal cube.
Optical components used for a 4-fluorophore imaging
Dye ND Filter*1Excitation Light Path Reflector Emission Light Path
Sapphire-pm 400DF15 535DF2
CFP-CaM — 440DF20 Glass 480DF30
YFP-mt 490DF20 535DF25
DsRed-nu 546DF10 595RDF60
*1ND filters in the holder of the illuminator.
Glass reflector captures fluorescence of multiple color dyes
Stray light reduction function equipped on all mirror units Ring slit illumination unit to reduce noise / IX2-RFRS
■Usage examples of the glass reflector
rThe ring slit illumination IX2-RFRS makes the ring shape
illumination on the objective to allow excitation light to pass through
the objectives outer portion
to not to excite the objective
auto-fluorescence generated
at the center of an objective.
Annular illumination
Auto-
fluorescence
Specimen
Objective
Ring slit
Excitation
filter Excitation
light
Hg lamp
Xe lamp
General observation
S
NN
Glass reflector transmission properties
*Solid line: excitation, Dotted line: emission
Excitation filter
Emission filter
Sapphire
CFP
YFP
DsRed
400 500
Wavelength (nm) 600 700
100
80
60
40
20
0
Transmittance (%)
Normal illumination Ring slit illumination
➔
Illumination Normal Annular
SIGNAL 408 479
NOISE 36 18
S/N 11.3 26.6
New FL system
The sharp performance of the dichromatic mirror in the new mirror unit minimizes crossover with the excitation filter and reduces
excitation light leakage to less than a tenth of our conventional models. Combined with the light absorbing mechanism (which absorbs
more than 99% of stray light), a high S/N ratio is achieved without the need for any special mechanism to prevent excitation light leakage.
IX2-RFRS and IX2-RS40/60/100
wOlympus has developed outstanding filter coating technology,
which gives the high efficient transmission and the reflection as well
as sharp cut off characteristics. This newly developed coating
results in optimized mirror units for the various fluorochromes
included ECFP/EGFP/EYFP/DsRed.

2/3 inch CCD
1/2 inch CCD
Projection area
109
[Fluorescence illuminator/IX2-RFA ]
Straight type illuminator designed for
maximum throughput is 20% brighter than
the previous model. Well suited for
applications requiring high intensity
excitation or multiple excitation filters.
The field stop (FS) is built in.
[L-shaped fluorescence illuminator/IX2-RFAL ]
Provides easy access to burner centration
and removable aperture and field stops.
The L-shaped design maintains access to
both back frame ports.
Bright excitation illumination for cell observation/manipulation
The Olympus lineup for fluorescence illumination equipment meets a wide variety of needs
including multi-color fluorescence, ratio imaging, photobleaching and uncaging observa-
tions. The brightness at low magnification is greatly improved.
A wide range of accessories to enable different kinds of
fluorescence imaging.
[Pinhole field stop/IX2-RFSPOT ]
Flexible field stop options IX2-RFSPOT pinhole field stop module
can be mounted in the L-shaped illuminator for photobleaching
experiments.
*Use commercially available pinhole plate
[Rectangular field stop/U-RFSS ]
This unique field stop allows the user to control the area of
fluorescence excitation anywhere inside the visual field. For
example, photobleaching and phototoxicity can now be limited to
only the area that is being imaged by the CCD improving overall
brightness and cell viability over long term observations. The unit is
attached at the field stop position of the fluorescence illuminator
IX2-RFAL.
[Double lamp housing adapter/U-DULHA ]
Allows simultaneous attachment of two light
sources such as halogen and mercury.
Selection mirror
is replaceable
for custom
applications.
Fluorescence illumination light source Reflected light fluorescence illuminators Illumination modular units
Configuration example
New FL system
Unnecessary exposure area
caused by a round field stop
[Double lamphouse illuminator/IX2-RFAW ]
Two light sources can be used
simultaneously, so that light stimulation can
be performed during observation.
Lamp housings
Shape Model Aspherical*1
Apochromatic *2Average Lamp IR
optics lens lamp life centering illumination
100 W mercury apo
lamp housing/ √√300 h Required Good
U-LH100HGAPO
100 W mercury
lamp housing/ √300 h Required Good
U-LH100HG
75 W xenon apo
lamp housing/ √√200 h Required Excellent
U-LH75XEAPO*3
*1: Can collect light more efficiently than conventional aspherical optics.
*2: Even illumination and no lamp focusing shift, even when changing excitation light wavelengths.
*3: Suitable for multi-color staining or ratio imaging because of flat light source spectrum.
[ C mount camera adapters/U-TV0.35XC-2, U-TV0.5XC-3,
U-TV0.63XC, U-TV1X-2 , U-TV1XC]
These low-magnification camera adapters cover from visible light to
near infrared red wavelength spectrum. U-TV0.35XC-2,
U-TV0.5XC-3, U-TV0.63XC and U-TV1X-2 are attached to the left
side port.
IR camera adapters
Practical field of view (mm) = Projection area (Field Number)
Objective magnifications
2/3 inch CCD 1/2 inch CCD 1/3 inch CCD
U-TV0.35XC-2 0.35X — 22 17.1
U-TV0.5XC-3 0.5X 22 16 12
U-TV0.63XC 0.63X 17.5 12.7 9.5
U-TV1X-2 1X 11 8 6
U-TV1XC 1X 11 8 6
Camera adapter
(Projection lens) Projection magnifications Projection area (F.N.)
U-TV0.63×C
U-TV0.35×C-2 U-TV0.5×C-3
U-TV1×CU-TV1×-2+
U-CMAD3

1211
Water immersion DIC condenser IX2-DICD + water immersion
top lens IX2-TLW
IX2-GS
High performance DIC condenser designed
for excellent optical performance and
specimen access in high magnification
observations. Designed for specimen
access, all controls are front mounted
including prism exchange and aperture
control. Three high numerical aperture top
lenses are available including the water
immersion IX2-TLW that offers 0.9 N.A.
with 3.7 mm of working distance and a 40°
approach angle for micro manipulations.
40°
This is the condenser adapter for upright
microscope condensers on the IX2,
including the 8-position turret condenser
(U-UCD8-2) for maximum system flexibility.
This combination allows the use of various
optional element with high N.A., just
rotating the smooth turret for switching
them easily. The IX2 illumination pillar also
offers a 'condenser-only' tilt mechanism to
quickly allow access to the
specimen without tilting
the entire illumination pillar.
* IX2-TLW cannot be used for U-UCD8-2
The Gliding Stage was designed for quick
rotation of the specimen using your
fingertips. With 20 mm of X-Y travel,
360 degree rotation and completely flat
surface, a specimen such as the nematode
worm C. elegans can be quickly brought into
the correct position and alignment for
injection or micromanipulations.
Nomarski DIC system offers the choice of optimal resolution or
high contrast in live cell observation.
Live cells specimens vary in thickness from from that of a nematode
worm such as C. elegans to a monolayer of cultured cells. The
requirements for DIC are also varied according to the specimen
from thinner cells being almost invisible to thicker specimens having
a lot of inherent contrast. Olympus provides three DIC systems with
varying amounts of shear. Small shear, high resolution sets are
excellent for thicker specimens. High contrast prism with twice the
normal shear are excellent for very thin specimens.
■Comparison of thick specimen (C. elegans), showing differences in shearing value
■Comparison of thin specimen, showing differences in shearing value
■ HR/HC optical elements for IX2-LWUCD
and applicable objectives
■ General type optical elements for
IX2-LWUCD and applicable objectives
■Selecting the optimum DIC prism optimum for specimen
thickness and objective magnification
■ DIC sliders
DIC observation using U-DICTHR
Shift DIC sliders for transmitted light/
U-DICTS
DIC sliders for transmitted light/U-DICT
High contrast DIC slider for transmitted
light/U-DICTHC
High resolution DIC slider for transmitted
light/U-DICTHR
DIC observation using U-DICT/ U-DICTS
DIC observation using U-DICTHC DIC observation using U-DICT/ U-DICTS
Thin specimen
(Big shearing value)
Thick specimen
(Small shearing value) 10X 40X 100X
U-DICTHC
for superior contrast with thin specimen
observation
U-DICTHR
for superior resolution with thick specimen
observation
U-DICT, U-DICTS
for general observation
• New DIC system gives a wider choice
UIS2 expands the selection of DIC
applicable objectives. Each condenser
prism is compatible with more lenses
making setup and configuration easier.
Differential Interference Contrast
DIC
DIC
Long working distance universal condenser/IX2-LWUCD
Water immersion DIC condenser/IX2-DICD
Condenser adapter/IX-ADUCD
Gliding stage/IX2-GS
Combining a long working distance (27 mm) and a high numerical
aperture (N.A. 0.55), the LWUCD condenser accommodates most
incubation chambers and T-Flasks. The 5-position turret provides
versatility with DIC or phase inserts. DIC components are specially
designed to obtain high-contrast, high-resolution images with 20X
and 40X objectives.
Polarizer
Condenser
Specimen
Objective
DIC prism
Analyzer
DIC prism
Shearing value
■Simple principle of Nomarski DIC
microscopy
Nomarski DIC amplifies contrast by using the
phase difference which occurs when light
passes through material with different
refraction or thickness value (e.g. a cell) in a
particular medium (e.g. water). The wave
direction of light from the microscope light
source is unified in a polarizer (condenser
side); and when it passes through the
condenser side DIC prism, it separates into
two beams which cross each other at right
angles. The distance of separation is called
the shearing amount. When two such
separated beams pass through a medium
with different refraction values (e.g. a cell), one
of them is delayed; and when the two beams
are re-composed by DIC prism (the observa-
tion side) and pass through the analyzer, the
interference effect produces the contrast.
This is the principle of Nomarski DIC.
Olympus has developed the most suitable
DIC prisms for different types of specimen,
based on the shearing amount. When DIC
contrast is low, the specimen is hard to
observe, while high contrast also hinders
observation because of excessive glare.
Olympus has therefore developed three
different types of DIC prisms to ensure clear
observation for every kind of specimen.
New DIC system
DIC elements Applicable objectives
IX2-DIC20HR UPLSAPO20X
IX2-DIC20HC UPLFLN20X
LUCPLFLN20X
IX2-DIC40HR UPLSAPO40X2
IX2-DIC40HC UPLFLN40X
UPLFLN40XO
LUCPLFLN40X
DIC elements Applicable objectives
IX2-DIC10 UPLSAPO10X2
UPLFLN10X2
IX2-DIC20 UPLSAPO20X
UPLFLN20X
LUCPLFLN20X
IX2-DIC40 UPLSAPO40X2
UPLFLN40X
UPLFLN40XO
LUCPLFLN40X
IX2-DIC60 PLAPON60XO
UPLFLN60X
UPLFLN60XOI
LUCPLFLN60X
IX2-DIC100 UPLSAPO100XO
UPLFLN100XO
UPLFLN100XOI
Numerical Aperture Working Distance Immersion
(N.A.) (W.D.)
IX2-TLW 0.9 3.7 mm Water
U-TLD 0.9 1.5 mm
U-TLO 1.4 0.63 mm Oil
■Top lens combination

13 14
A wide lineup of UIS2 objectives.
Relief contrast equipment
Phase contrast equipment
This universal condenser for phase contrast and brightfield
observations offers excellent workability due to its long working
distance (73 mm) and compatibility with large containers: it can be
used in combination with 4X -40X phase contrast objectives.
Phase contrast observation is also possible with
the IX2-LWUCD condenser, whose working
distance is 27 mm.
The Olympus Relief Contrast system provides a high contrast,
3-D image similar to DIC for specimens mounted in plastic vessels.
Relief contrast is designed for use in cellular fertilization and making
the nuclear envelope easier to see and penetrate.
Relief contrast equipment
* Unifies the shadow directions of each objective, improving
operability at all magnifications.
* A long working distance (45 mm) for the condenser (IX2-MLWCD)
doesn't bother the operation of the manipulator.
Two types of objectives for relief contrast are selectable: cost-
efficient Achromat models, or PlanSemiApochromat objectives with
high resolution and excellent focusing right up to the image
perimeters. Condenser (IX2-MLWCD) also supports DIC and phase
contrast observations for maximum flexibility.
The IX2-MLWCD equipes with optical component RC1 (for 10X objective), RC2 (for 20X objective),
RC3 (for 40X objective) and a polarizer to adjust the contrast.
PH
Special equipment for
relief contrast and phase contrast.
Ultra long working distance condenser/IX-ULWCD
RC
RC
PH
RC/PH System UIS2 Objectives
Mouse embryo
Optical elements for Applicable objectives
IX2-MLWCD
IX2-MPHL UPLFLN4XPH
IX2-MPHC CPLFLN10XPH, CPLN10XPH, LCACHN20XPH
IX2-MPH1 LUCPLFLN20XPH
IX2-MPH2 LUCPLFLN40XPH, LCACHN40XPH, LUCPLFLN60XPH
Optical elements Objectives
IX2-MDIC20 UPLSAPO20X, UPLFLN20X, LUCPLFLN20X
IX2-MDIC40 UPLSAPO40X2, UPLFLN40X, UPLFLN40XO* ,
LUCPLFLN40X
N.A. W.D.
CPLN 10XRC *1 0.25 9.7 mm
LCACHN 20XRC *1 0.4 2.8 mm
LCACHN 40XRC *1 0.55 1.9 mm
CPLFLN 10XRC *1 0.3 9 mm
LUCPLFLN 20XRC *2 0.45 6.6 — 7.8 mm
LUCPLFLN 40XRC *2 0.6 3.0 — 4.2 mm
Achromat for
Relief
Contrast
Plan Fluorite
for Relief
Contrast
■Objectives for Relief Contrast observation
■Phase contrast optical elements for IX2-MLWCD
and applicable objectives
■DIC optical elements for IX2-MLWCD and
applicable objectives
*1 Objective with compensation for 1 mm plastic dish plus 0.5 mm
thick thermoplate
*2 Objective with compensation ring for 0~2 mm thick cover glass.
* Use with shift DIC slider (U-DICTS).
Model N.A. W.D. F.N. Cover glass Immersion Spring Correction Iris Water proof &
(mm) thickness (mm) ring diaphragm oil proof function
UPLSAPO UPLSAPO 4X 0.16 13 26.5 —
UPLSAPO 10X2 0.4 3.1 26.5 0.17
UPLSAPO 20X 0.75 0.6 26.5 0.17 _
UPLSAPO 20XO 0.85 0.2 26.5 — Oil _
UPLSAPO 40X2 0.95 0.18 26.5 0.11-0.23 __
UPLSAPO 60XW 1.2 0.28 26.5 0.13-0.21 Water __ _
UPLSAPO 60XO 1.35 0.15 26.5 0.17 Oil __
UPLSAPO 100XO 1.4 0.13 26.5 0.17 Oil __
PLAPON PLAPON 60XO 1.42 0.15 26.5 0.17 Oil __
PLAPON 60XOSC 1.40 0.12 22 0.17 Oil __
UPLFLN UPLFLN 4X 0.13 17 26.5 —
UPLFLN 10X2 0.3 10 26.5 —
UPLFLN 20X 0.5 2.1 26.5 0.17 _
UPLFLN 40X 0.75 0.51 26.5 0.17 _
UPLFLN 40XO 1.3 0.2 26.5 0.17 Oil __
UPLFLN 60X 0.9 0.2 26.5 0.11-0.23 __
UPLFLN 60XOI 1.25-0.65 0.12 26.5 0.17 Oil ___
UPLFLN 100XO2 1.3 0.2 26.5 0.17 Oil __
UPLFLN 100XOI2 1.3-0.6 0.2 26.5 0.17 Oil ___
LUCPLFLN LUCPLFLN 20X 0.45 6.6-7.8 22 0-2 _
LUCPLFLN 40X 0.6 2.7-4 22 0-2 _
LUCPLFLN 60X 0.7 1.5-2.2 22 0.1-1.3 _
LUCPLFLN 20XPH 0.45 6.6-7.8 22 0-2 _
LUCPLFLN 20XRC 0.45 6.6-7.8 22 0-2 _
LUCPLFLN 40XPH 0.6 3.0-4.2 22 0-2 _
LUCPLFLN 40XRC 0.6 3.0-4.2 22 0-2 _
LUCPLFLN 60XPH 0.7 1.5-2.2 22 0.1-1.3 _
UPLFLN-PH UPLFLN 4XPH 0.13 17 26.5 —
UPLFLN 10X2PH 0.30 10 26.5 —
UPLFLN-PHP UPLFLN 4XPHP 0.13 16.4 22 —
CPLFLN CPLFLN 10XPH 0.3 9.5 22 1
CPLFLN 10XRC 0.3 9 22 1.5
LCACHN LCACHN 20XPH 0.4 3.2 22 1
LCACHN 20XPHP 0.4 3.2 22 1
LCACHN 20XRC 0.4 2.8 22 1.5
LCACHN 40XPH 0.55 2.2 22 1
LCACHN 40XPHP 0.55 2.2 22 1
LCACHN 40XRC 0.55 1.9 22 1.5
CACHN & CPLN CACHN 10XPHP 0.25 8.8 22 1
CPLN 10XPH 0.25 10 22 1
CPLN 10XRC 0.25 9.7 22 1.5
UAPON 340 UAPON 20XW340 0.70 0.35 22 0.17 Water __
UAPON 40XO340 1.35 0.1 22 0.17 Oil __
UAPON 40XW340 1.15 0.25 22 0.13–0.25 Water __ _
TIRF APON 60XOTIRF 1.49 0.1 22 0.13-0.19 Oil __
UAPON 100XOTIRF 1.49 0.1 22 0.13-0.19 Oil __
UAPON 150XOTIRF 1.45 0.08 22 0.13-0.19 Oil __
◆All UIS2 objectives and WHN eyepieces: lead-free eco-glass.
UIS2 objectives
Model N.A. W.D. F.N Cover glass Immersion Spring Correction Iris Water proof &
(mm) thickness (mm) ring diaphragm oil proof cap
TIRFM APO 100XOHR* 1.65 0.1 22 0.15 Oil __
*Special cover glass and immersion oil required.
UIS objectives

1615
Light adjustment dial
Focus knob
TTL Pulse control switch
ON/OFF switch for the
motorized shutter (e.g.
made by UNIBLITZ).
Light path selection lever
Two-stage selection
between the observation
tube and the left side port.
This big lever prevents
operation errors in the dark
room.
Power ON/OFF switch
Aperture stop (A.S.)
Field stop (F.S.)
Shutters for
fluorescence excitation
[Frontal control ]
High level basic performance makes a vital difference to
experiment results.
[Compact body ]
Compact body design allows wide port space for both left and right
sides, the bottom and on the back. It allows you to use the variety
of peripherals with offering an
excellent operability.
290 135
394
245
[Tilting binocular tube/U-TBI90 ]
A tilting observation tube with 35-85°
elevation angle. This tube offers
ergonomic operation for both sitting and
even standing position.
[Focus free collection ring ]
The newly developed LUCPLFLN40X (N.A.
0.6, W.D. 3.4 mm*) and LUCPLFLN60X (N.A.
0.70, W.D. 1.5-2.2 mm) are compatible with
various container thickness. Turning the
correction ring does not blur the focus when
correcting spherical aberration caused by
different container thickness. A simple
correction operation optimizes the
observation image. * When using 1 mm thickness container.
■LUCPLFLN 40X
Operating the correction ring does
not blur the focus.
■When using a conventional
objective with correction ring
Focus blurs when correction ring
is operated.
[ Oil immersion
protection function ]
Prevents immersion oil
infiltrating through the tip of
the objective.
[Fluorescence turret
confirmation window ]
The fluorescent mirror unit
can be confirmed from the
space between the left and
right eyepieces of the
observation tube.
[
Fluorescence indicator
]
Bright,easy-to-see self-
illuminated labels are used
to denote fluorescence filter
sets, easily visible in a dark
room.
[ Glass stage insert
plate/IX2-GCP ]
The objective type and its
magnifications can easily be
recognized through this
glass stage insert.
[ Magnification changer ]
This intermediate
magnification changer
offers different
magnification without
switching the objective lens.
1.6X is standard (IX71/81)
and 2.0X is optional.
In addition to maximizing rigidity of IX2 microscope frame, Olympus
simplified or shortened mechanical structures from the focusing
handle to the revolving nosepiece, thereby achieving to minimize
the focus drift.
In order to minimize light loss from reflection, a simple V-shaped
optical system is employed. This restricts reflection inside the
microscope to one-time-only, reducing light loss and allowing
observation of even weak fluorescent signals.
■Thermally compensated relay lens optics
Used for the observation optical path, thermally-compensated relay
lens optics involve combining lenses with different thermal
characteristics to offset blurs caused by temperature change.
■External power supply
Time-lapse observation over a long period will cause some heat
strain to the microscope, from temperature changes in the
environment and air blown from an air-conditioner. Because such
changes can cause blurring, the IX2 series design focuses on its
structure in order to maximize rigidity and equips the external
power supply for transmitted illumination on the outside of the
microscope. Thereby IX2 series archives the highest level of thermal
expansion compensation. Various accessories are provided to
stabilize long-term time-lapses, such as an incubator that reduces
temperature changes in the environment and the effects of air
conditioning.
Since the UIS2 optical system is compensation-free (i.e. compensa-
tion is performed only by the objective lens), a clear primary image*
can be captured through any camera port offering from IX2 system.
* The "primary image" is the first image created by convergence of the luminous flux after passing
through an objective. There is no loss in light quantity and no image deterioration.
Capture of high-clarity primary image
V-shaped optical path to reduce light loss
Against thermal expansion to prevent defocusing
High body rigidity
Revolving nosepiece
guide structure
The shorter section in red,
the less influence from
heat and force — resulting
in improved rigidity.
Thermally
compensated
relay lens optics
Ease of use in a compact body
Basic Characteristics
(Unit: mm)

1817
Motorized system for live cell imaging.
Motorized shutter/IX2-SHA
Can be mounted in both transmitted and
reflected light paths.
Motorized filter wheel/
U-FWR and U-FWO
6 positions motorized filter wheel is offered
for both excitation and observation.
Microscope front panel
Easy to use buttons allow selection of light
path, light intensity and lamp on/off control.
Auxiliary buttons can be custom
programmed. Includes LED lamp intensity
meter.
Nearly every operating function on the IX81 can be allocated to operation
buttons on the PC, the hand switch and the microscope in any individual or
multiple combinations by using IX2-BSW* control software. Some image
analysis software can also be used to control microscope operation, image
capturing and analysis; in this case, all operations are done from a single
PC.
* Included with the system controller IX2-UCB2
Example : Switching from fluorescence observation to Nomarski DIC.
Motorized sextuple
revolving nosepiece
Up to 6 objectives are mounted
simultaneously, included with microscope
frame.
Internal motorized focus drive
With minimum movement of 0.01 µm,
the user has precise focus control.
Motorized fluorescent cube
turret/ IX2-RFACA
Accepts up to 6 fluorescence filter cubes,
making it easy to switch between them
during fluorescence observation of
multistained specimens. (Manual shutter
included)
Handset/U-HSTR2
A remote handset controls all motorized
functions via a convenient and
programmable interface.
Focus handle/U-FH
The remote focus handle duplicates the feel
and function of the microscope's focus
knobs. Additional controls include
fine/coarse focus selection, lamp on/off,
shutter open/close, and camera vs. visual
observation.
■Parfocal compensation function among objectives
This function allows the focus point to be matched from
low to high magnification objectives. Refocusing each time
the magnification is changed is no longer necessary.
■Malfunction prevention
Motorized units ensure that complicated operations are
performed without error. Once the usage conditions are
set, the setting screen can be hidden to avoid accidental
change leading to faulty operation.
■Setting sensitivity of the fine focus movement for
each objective magnification
Users can set the amount of the fine focus movement per
rotation of the focus adjustment knob.
■Save setting conditions for each operator
Customized data can be stored in folders, and each folder
labeled for different users or sets of conditions.
System controller/IX2-UCB2
All motorized units are powered by this
external system controller. Included is an
RS232C connection for PC commands and
expansion slots for future system upgrades.
Motorized bottom
port unit with
C-mount/IX2-TVRAC
Objective escape and
zero-return buttons
Moves objective to lower focus limit.
Allows setting of default focus position.
* Equipped on each side of microscope frame.
Motorized universal condenser/
IX2-LWUCDA2
This condenser has six built-in optical
components to enable brightfield, phase
contrast and Nomarski DIC observations.
Software allows switching optical
components to be synchronized with the
objective. (Manual AS included.)
Controlling functions via PC, handset or operating buttons on the microscope body
e Open shutter for transmitted
illumination
w Exchange FL mirror unit for
DIC mirror unit
qClose shutter for fluorescence
illumination
With the IX81, this
sequence of
functions can be
allocated to
a single button
U-FWO
U-FWR
Functions of IX81 control software /IX2-BSW
Motorized Units

20
[ Frame plate adapter/
IX2-FP ]
This is used to fix the
microscope frame
to the anti-
vibration stand.
*Screws (available
separately) are required for
fixing.
[ Nosepiece stage/IX2-NPS]
This simple mechanical stage is designed for long time observations to minimize the
distance change between the specimen and the objective in other words ‘focus drift’.
It works by minimizing the effect of temperature change and prevents blur during long
observations. Attach one objective in use.
[Incubators ]
This box type incubator keeps the microscope temperature stable
with enclosing many components inside the box. Please see your
local supplier for more detail.
[ Comparison of normal observation images ]
Change of focus when environmental temperature (25°C )
changes by ±5°C. *When the microscope is used without an incubator.
Accessories to improve stability in long-duration observations
[ CO2incubators/MIU-IBC-I, MIU-IBC-IF ]
Highly precise incubator control keeps the environment inside a
laboratory dish completely stable, at just below 37°C temperature,
90% moisture and 5% CO2concentration (when using a CO25%
concentration bomb); in this way, live cell activity can be maintained
for about 2 days. A special designed structure is employed to
minimize the focus drift during temperature control. This is the ideal
solution for time-lapse experiments under both a confocal laser
scanning microscope and a wide field
observation. The opening hole located on
the top heater is available for the cell
injection.
* Built-in stage warming plate
* Objective heater
* 5% CO2 supply tube with ø4 outer diameter, ø2 inner diameter
and 400 mm length.
* Not available in some areas
[ Thermoplate/MATS series ]
This thermoplate maintains the
temperature of the sample at 37°C.
* Tokai Hit Company products
MIU-IBC-IF
High grade configuration with a built-in
flowmeter for 5% CO2and 95% air. Use the 5%
CO2and 95% air bombs.
MIU-IBC-I
Basic configuration for control of heaters for
top, bath, stage and objective.
[ Comparison of fluorescence images ]
Time-lapse observation images.
*When the microscope is used without an incubator.
With IX2-NPS
Normal observation images
With IX2-NPS
Normal observation images
20°C 25°C 30°C 0 30 60 120 minElapsed timeEnvironmental temperature
Live Cell Imaging System
19
Maintaining long-term stability for live cell observation.
Focus drift compensation function for
time-lapse experiments.
Motorized inverted research microscope with
focus drift compensation/IX81-ZDC
This landmark microscope model makes it easy to reproduce any
preset focus position. 785 nm weak laser light is introduced
through the additional optical path between the tube lens and an
objective to measure the distance between the objective and the
reflection plane, which is normally the boundary of the reflective
index difference such as the boundary between cover glass and
cell. Therefore this system never cause unnecessary
photobleaching of the specimen.
Live cell imaging system
B.
Focusing
position
Reflection
plane
Objective Light
shielding
plate
Split detector
Telan lens
Laser light
source
(class 1)
A.
Closer
than focus
position
C.
Further
than focus
position
* Extended image

Confocal laser scanning microscope/
FLUOVIEW FV1000 system
The Fluoview/FV1000 is a next-generation imaging system
designed for high-resolution, confocal observation of both fixed and
live cells. The FV1000 offers advances in confocal system
performance while providing the speed and sensitivity required for
live cell imaging with minimal risk of damage to living specimens.
In addition, the FV1000 offers a revolutionary synchronized laser
scanning system called the SIM Scanner.
While one laser stimulates, the second laser
simultaneously provides high resolution
imaging. This coordination of laser
stimulation and imaging makes the FV1000
an ideal choice for FRAP,
FLIP and photoactivation.
* FV1000 is a class 3B laser product.
2221
Manipulator
Manipulating cells.
Micromanipulation system/ON3
Olympus' original micromanipulator offers high stability and
excellent stability because of its compact body. Motorized coarse
and oil hydraulic fine three axes operation are designed in its
compact and rigid body with hanging down ergonomic joystick
control. ON3 micromanipulator is securely fixed through the screw
holes on IX2 microscope frame.
ON3-99D ON3-99D with return mechanism (UT-R) Manual combination
(ONM-2D+ONO-301D+UT-D) Manual combination with return mechanism (ONM-2D+ONO-301D+UT-D+UT-R)
Human embryo Images of Kaede-expressed cells demonstrating
the photoactivation acquired every 300 msec and
observed via 405 blue diode laser illumination with
twin scanners.
TIRFM
(Total Internal Reflection Fluorescence
Microscopy)
Since 1997, Olympus has been a market leader in objective based
Total Internal Reflection microscope that allows an evanescent
wave illumination approximately 200 nm into the specimen beyond
the coverglass interface. Olympus extends that leadership role by
offering four objectives for TIRFM including the world's highest N.A.
objective, the 100X N.A. 1.65 objective.
The incredibly thin optical section created be TIRFM allows an
extremely high signal to noise image to be collected. Popular
applications include vesicle tracking, cellular adhesions and single
molecule events.
• Olympus' original high N.A. objectives make it easy to produce an
evanescent wave field. So little light is leaked that a high-contrast
image can be obtained against a dark background.
◆N.A. 1.65, 100X objective (APO100XOHR)
(Use special cover glass and immersion oil)
◆N.A. 1.49, 60X objective (APON60XOTIRF)
(Use normal cover glass and immersion oil)
◆N.A. 1.49, 100X objective (UAPON100XOTIRF)
(Use normal cover glass and immersion oil)
◆N.A. 1.45 150X objective (UAPON150XOTIRF)
(Use normal cover glass and immersion oil)
• Once the initial alignment of the laser
optical path is set, it is just simple operation
switching between TIRF and widefield
illumination.
* TIRFM is a class 3B laser product.
Ultra-sensitive fluorescence microscopy.
Micrometer
TIRFM
Total Internal Reflection Fluorescence
observation with evanescent wave excitation Widefield fluorescence observation with mercury arc lamp excitation
Simultaneous laser light stimulation and imaging.
Laser Scanning Confocal Application System

2423
TIRFM (Total Internal Reflection Fluorescence
Microscopy) system with arc lamp source
Featuring the Olympus-developed total internal reflection
illumination system and slit mechanism to provide evanescent wave
illumination from an arc lamp source. High signal to noise
fluorescence observations with extremely thin optical sectioning can
now be easily performed at the specimen-coverslip interface.
The arc lamp is focused on an off-center slit using a wedge prism
and focused on the outer edge of the back focal plane of the
objective, thus causing the excitation light to exit the objective
beyond the critical angle resulting in Total Internal Reflection.
The wedge prism and slit can be easily removed from the light path
via a slider for wide field fluorescence observation. Through the use
of filters, this system
enables a wider choice of
excitation colors than
current laser base
system.
Conventional fluorescence observation
Microtubule of an NG108-15 cell labeled with Alexa488 through indirect fluorescence antibody test
TIRFM observation
High-precision fluorescence turret /IX2-RFACEVA
Turret includes three, highly precise,
empty fluorescence filter cubes that
permit dichromatic mirror switching
while maintaining excitation light
position on the back focal plane of
the objective. This system makes
multi-color observations easy and
alleviates the additional adjustment
of the excitation source when
switching mirror units. Up to six
mirror units can be installed.
Off-center slit slider
Excitation filter slider
Fluorescence lamp housing
L shape fluorescence
illuminator
Exclusive
objective
High precisiton
fluorescence turret
Wedge prism slider
LIFETIME
BURNERON
LIFETIME
BURNERON
U-LH75XEAPO
75 W xenon apo
lamp housing
U-RFL-T
Power supply unit
for mercury lamp
U-RX-T
Power supply unit
for xenon lamp
U-LH100HG
100 W mercury
lamp housing
U-LH100HGAPO
100 W mercury apo
lamp housing
Mirror unit
IX2-RFAL
L shape fluorescence
illuminator
IX2-RFAC
Fluorescence turret
Camera
adapter
High
sensitive
camera
IX71
Research
inverted
system
microscope
A
PON60XOTIRF
objective
Exclusive
vacant mirror unit
High-precision
fluorescence turret
Excitation filter slider
Motorized filter wheel
Wedge prism slider
Off-center slit slider
IX2-RFACEVA
IX2-ARCEVA
Kaede-Crk II protein expressed in a HeLa cell
IX2-ARCEVA
World’s first evanescent illumination system from an arc lamp source.
ARC EVA
SYSTEM DIAGRAM
Main specifications
Microscope Research inverted system
microscope IX71
Fluorescence Arc illumination total internal reflection
illuminator fluorescence unit IX2-ARCEVA
(Slit slider, wedge prism slider and
excitation filter slider)
L-shape fluorescence illuminator
IX2-RFAL
Mirror unit cassettes High-precision fluorescence turret
(choose from either IX2-RFACEVA
fluorescence turret) (with centering mechanism and
3 vacant mirror units)
Fluorescence turret
IX2-RFAC
Lamp light source 100 W mercury lamp,
75 W Xenon lamp
Objective APON60XOTIRF
N.A. 1.49. W.D. 0.1 mm
Used with normal cover glass and
immersion oil
Stage Left short handle stage IX-SVL2
Total internal reflection 11
illumination F.N.
Observation Recommend high sensitive camera
Application System
Disk Scanning Confocal Microscope System
The Olympus Disk Scanning Unit (DSU) offers confocal images
using a white light, arc excitation source and CCD camera. The
heart of the system is a unique slit disk pattern, that offers excellent
light throughput and thinness of optical Sectioning. Compatible with
any IX71 and IX81.
• Compliance with various fluorochromes with different spectral
characteristics.
Since an arc light source is used, the unit can meet different
fluorochrome requirements across a wide wavelength spectrum by
simply switching a standard mirror unit.
• Minimize excitation light damage to the specimen and
maximize emission light throughput.
The excitation light volume is reduced to around 5% as a result of
passing through the disk. So, there is almost no fading of
fluorescence emission from the surface of the focused sample.
• Construction of 3D images.
Brilliant 3D image can be easily captured with excellent optical
sectioning with high precision motorized Z axis of IX81.
• Low and high magnification objective support.
Five DSU disks are available of varying slit spacing and width for the
wide variety of the objectives, included oil or water immersion high
N.A. objectives.
• Easy switching between confocal and reflected light
fluorescence observation .
IN/OUT of the confocal disk to or from the light path can be done
by a hand switch or via software, so it is easy to switch observation
methods between DSU and reflected light fluorescence.
Spinning Disk Confocal
Zebrafish 3-day embryo, ventral view, projection of 62 serial optical sections
Adult brain of Drosophila, reflected light fluorescence image (left) and DSU image (right)
Obtaining confocal images easily by use of an arc light source.
* Not available in some areas * Not available in some areas

2625
FRET Split imaging system
• Simultaneous two-color split imaging with one CCD camera.
• Unique design splits the primary image for the highest efficiency
and light transmission necessary for weak fluorescence signals
such as CFP/YFP FRET experiments.
•Compact and space-saving design takes advantage of the 70 mm
of free space between the microscope frame and the primary
image plane found on all Olympus Research Upright and Inverted
Microscopes.
• Simple cassette mechanism makes it easy to switch between
split and full frame imaging.
• Unit is up to 10% brighter than similar relay lens based, image
splitting systems.
• When used with the rectangular field stop U-RFSS, excitation
energy is limited to the camera's field of view, minimizing specimen
photo-bleaching.
Bright, simultaneous two-wavelength imaging using the primary image.
Cube cassette for
full image
Rectangular field
stop/U-RFSS
Split primary image
camera port/U-SIP
Cube cassette for
split images
Prism
Built-in separation
dichromatic mirror
(DM505)
Filter sliders
(Emission, ND sliders)
Filter set such as
XF88-2(OMEGA) or
31044v2(CHROMA)
Specimen
Objective
Excitation filter
Tube lens 75W xenon apo
lamp housing
U-LH75XEAPO
Power supply unit
Research inverted
system microscope
IX81/IX71
L shape fluorescence illuminator
IX2-RFAL
Fluorescence mirror unit
Split primary image
camera port
U-SIP
Cube cassette for
split imageMirrors
Split image
High resolution camera
YFP CFP
Rectangular field stop
U-RFSS
U-SIP main specifications
Microscope IX71/81
Image separation Right and left 2-separation
(can be adjusted independently)
Built-in separation dichromatic mirror DM505 (special size)
Filter slider Emission, ND filters' size ø25 mm,
total thickness: 8 mm
Used together with commercially available filter set
(XF88-2 OMEGA) or 31044v2(CHROMA)
Field Number Split image: 8
Full image: 11
Magnifications 1X (primary image)
Objectives 40X and higher
Camera mounting C-mount
Recommended camera Chip size 2/3 inch
YFP CFP
HeLa cell, in which YC3.1 (cytoplasm) and YC3.1nu (with nuclear localization signal) are coexpressed.
FRET changes are observed through histamine stimulation, and images are acquired at intervals of
50 msec.
FRET
Photoactivation Fluorescence
Microscope system
The photoactivation illuminator allows the exposure by UV light to
specific regions of a cell for photoconversion, the uncaging of
compounds and the photoactivation of specific fluorochromes.
• A specified area of the cell can be exposed to UV light while
observing the targeted cell by fluorescence or transmitted (DIC)
method.
• Compliance with FRAP or FLIP
experiments (by special order).
• Easy system upgrade by
attaching double lamphouse
illuminator IX2-RFAW to IX2
series inverted microscope.
Photoactivation illuminator for inverted microscopy.
The novel Kaede gene is useful in biology because it exhibits photoconversion. Normally, the Kaede
gene shows green fluorescence but after exposure to UV light will exhibit red fluorescence. By using
UV light to only a specific region within a labeled cell and then noting the movement of red beyond
that region, observations of internal cellular dynamics can easily be made. The photo on the left
shows a nerve cell (from a rat hippocampus) pre-labeled with green Kaede gene.
The photo on the right side was taken after the right-most cell body was exposed to a 10 µm
diameter spot of UV light for 60 seconds, thus changing the Kaede gene from green to red. Note the
translocation of the red shifted gene outside of the 10 µm spot thus indicating intracellular transport
mechanisms.
High resolution
camera
Excitation filter
BP330-385
Filter slider
Composed dichromatic
mirror of right and left path
Excitation filter
475AF20(XF1072 OMEGA) or
HQ475/20x(CHROMA)
Excitation filter *2
550DF30(XF1021 OMEGA)
Fluorescence mirror unit
U-MF2+dichromatic mirror
(DM400 on the illustration)
Objective
UPLFLN40XO
Filter wheel 2
such as Lambda10-2
Fluorescence filter *2
575ALP (XF3089 OMEGA) or
HQ575LP(CHROMA)
Fluorescence filter
530DF30(XF3107 OMEGA) or
D530/30x(CHROMA)
Filter slider
Inverted microscope
IX series
Pinhole slider
Pinhole or slit
Magnetic shutter
Sample
Filter wheel 1
such as Lambda10-2
Double lamp
house illuminator
IX2-RFAW
Field
stop
position*1
Double lamphouse illuminator IX2-RFAW
IX2-RFAW specifications
Microscope IX81/71/51, IX70/50
Pinhole slider 2-step exchange (pinhole or slit/vacant hole)
Pinhole and slit are available on the market
(ø16 mm Melles Griot Inc. products)
Exposed area on Pinhole diameter
the specimen objective magnification
Filter slider 3-step exchange (shutter/filter pocket/vacant hole)
BP330-385 excitation filter equipped
Excitation filter slider 5-step exchange (4-step filter pocket/vacant hole)
Filter size Excitation filter: ø25 mm,
thickness: 6 mm and below
ND filter: ø32 mm,
thickness: 1 mm and below
Composed dichromatic mirror DM400 (standard)
of right and left light path Slide IN/OUT type
Power consumption 7.4 A
Dimensions Width: 710 mm
Depth: 740 mm (from the front of tilting tube to
the end of the illuminator)
*1 Field stop position is the same
position as the focus point of the
sample.
*2 Use 550DF30 excitation filter in the
filter wheel 1 and 575ALP
fluorescence filter in the filter wheel 2
when observing red Kaede protein.
Exchange of the fluorescence mirror
unit is not required.
Photoactivation Application System
* Not available in some areas * Not available in some areas
Setting up example for Kaede

2827
45 3
431
368
690.5
308
290
135 584 394
IX71 dimensions (unit: mm) (unit: mm)
(unit: mm)
(unit: mm)
(unit: mm)
(unit: mm)
TH4 dimensions
IX2-UCB2 dimensions
U-HSTR2 dimensions
U-FH dimensions
Weight: 16 kg Power consumption: 200 VA
* Lengths with an asterisk (*) vary according to interpupilllary distance.
Weight: 20.2 kg Power consumption: 480 VA
* Lengths with an asterisk (*) vary according to interpupilllary distance. Weight: 21.2 kg Power consumption: 480 VA * Lengths with an asterisk (*) vary according to interpupilllary distance.
IX81 dimensions
Weight: 16.9 kg Power consumption: 350 VA
* Lengths with an asterisk (*) vary according to interpupilllary distance.
667
*406~471
*243~391
290
135 394
299
667
*406 ~471
*243~391
290
135
299
394
14.5
75 200
120
125
30°
64.3
48.6
38.0
91.5
50.0
75.5
4.0
70.0
35.5
82.328.3
54.0
146
7°
180
105
OB
Seemanual
151.4
212 216
125 15 310
332 (depth)
OFFON
SW2
SW1
HS
RS232C
ERR
NP
Z/AF
CDT
TL
FW3
FW2
FW1
AS
RSHT
MU
RMT
IX-UBC
IX71+fluorescence illuminator combination dimensions IX71+L-shaped fluorescence illuminator dimensions
IX81-ZDC dimensions
667
*406~471
*243~391
308
290
135 394 584
667
*406~471
*243~391
308
290
135 394
453
549
Microscope body Revolving nosepiece Sextuple, simple waterproof mechanism incorporated
Focus 9 mm stroke (from stage surface, 7 mm upward and 2 mm downward), coaxial coarse and fine focusing knobs (minimum fine
focus graduation: 1 µm, full rotation of fine focusing knobs: 100 µm), upper limit stopper, torque adjustment for coarse focusing
Primary image port Lower port (standard left side port: S1F 100% or S8F 80%, or optional lower Back port selectable, 2-step light path selection),
Upper port when built-in magnification changer 1X/1.6X is replaced (optional right side port),
Bottom port (option)
Frontal operation Light path selector, Transmitted light intensity control and light ON/OFF switch, TTL Pulse control switch
Transmitted light 100 W transmitted light IX2-ILL100 Pillar tilt mechanism (30° inclination angle, with vibration reducing mechanism),
illuminator illumination pillar Condenser holder (with 50 mm stroke, swing-in/out mechanism),
Field iris diaphragm adjustable, 4 filter holders (ø45 mm, t=6 mm or less)
External power supply unit TH4-100/200 Two versions available (100 V and 200 V), Optional TH4-HS hand switch can be used, 2.2 kg weight
Observation tube Tilting binocular tube U-TBI90 35-85° continuous angle adjustable (eyepoint height range: 406 mm-471 mm),
interpupillary distance adjustable between 50-76 mm, diopter adjustment function, erect image, F.N. 22
Binocular tube U-BI90CT Built-in focusing telescope, interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
U-BI90 Interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
Trinocular tube U-TR30H-2+IX-ATU 3 step optical path selectable (observation : straight port = 100:0, 20:80, 0:100),
interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
Stage Cross stage with flexible right handle IX2-SFR 50 mm (X) X 50 mm (Y) stroke, stage insert plate exchangeable (ø110 mm)
Cross stage with short left handle IX-SVL2 50 mm (X) X 43 mm (Y) stroke, stage insert plate exchangeable (ø110 mm)
Plain stage IX2-SP 232 mm (X) X 240 mm (Y) stage size, stage insert plate exchangeable (ø110 mm)
IX-MVR Mechanical stage to be used with IX2-SP, 130 mm (X) X 85 mm (Y) stroke
Narrow plain stage IX2-KSP 160 mm (X) X 240 mm (Y) stage size, stage insert plate exchangeable (ø110 mm)
CK40-MVR Mechanical stage to be used with IX2-KSP, 120 mm (X) X 78 mm (Y) stroke
Gliding stage IX2-GS Upper circular stage 360° rotatable, 20 mm (X/Y) travel
Condenser Long working distance universal IX2-LWUCD 5 positions for optical devices (3 positions for ø30 mm and 2 position for ø38 mm),
aperture iris diaphragm adjustable, N.A. 0.55 / W.D. 27 mm
Long working distance Relief Contrast IX2-MLWCD 4 positions for optical devices (for ø50 mm, Relief Contrast optical devices rotatable),
aperture iris diaphragm adjustable, N.A. 0.5 / W.D. 45 mm
Ultra long working distance IX-ULWCD 4 positions for optical devices (for ø29 mm), aperture iris diaphragm adjustable, N.A. 0.3 / W.D. 73 mm
Water immersion DIC IX2-DICD + IX2-TLW Single position for optical device (include two optical device holders),
40° injection pipette or electrode insertion angle, aperture iris diaphragm adjustable, N.A. 0.9 / W.D. 3.7 mm
Eyepiece WHN10X High eyepoint, F.N. 22
WHN10X-H High eyepoint, diopter adjustment function, F.N. 22
Reflected light Fluorescence illuminator IX2-RFAL L-shaped design with exchangeable F.S. and A.S. modules, two filter holder sliders (2 positions, ø32 mm, t=6 mm or less)
fluorescence unit IX2-RFA Straight design with field iris diaphragm, filter holder slider (2 positions, ø32 mm, t=6 mm or less)
Fluorescence cube turret IX2-RFAC 6 positions in a rotating turret, built-in shutter
Light source 100 W Hg lamp housing and transformer, or 75 W Xe lamp housing and transformer
IX71 specifications
Microscope body Revolving nosepiece Sextuple motorized with objective lens retraction in PC mode,
simple waterproof mechanism incorporated
Focus 9 mm stroke (from stage surface, 7 mm upward and 2 mm downward), fine/coarse switchable focusing knobs (minimum
graduation: 0.01 µm), objective lens escape/return buttons and return to memory position buttons (each side of microscope
frame)
Primary image port Lower port (standard left side port: S1F 100% or S8F 80%, or optional lower Back port selectable, 2-step light path selection),
Upper port when built-in magnification changer 1X/1.6X is replaced (optional right side port),
Bottom port (option)
Frontal operation Light path selector button, Transmitted light intensity control buttons and light ON/OFF switch button, Fine/Coarse focus
selector button, TTL Pulse control switch (auxiliary) buttons
Transmitted light 100 W transmitted light IX2-ILL100 Pillar tilt mechanism (30° inclination angle, with vibration reducing mechanism),
illuminator illumination pillar Condenser holder (with 50 mm stroke, swing-in/out mechanism), Field iris diaphragm adjustable,
4 filter holders (ø45 mm, t=6 mm or less)
External power supply unit IX2-UCB2 Auto voltage selector (100 V / 200 V), RS232C interface for PC operation, IX2-BSW driver software
Observation tube Tilting binocular tube U-TBI90 35-85° continuous angle adjustable (eyepoint height range: 406 mm-471 mm),
interpupillary distance adjustable between 50-76 mm, diopter adjustment function, erect image, F.N. 22
Binocular tube U-BI90CT Built-in focusing telescope, interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
U-BI90 Interpupillary distance adjustable 50-76 mm, diopter adjustment function, F.N. 22
Trinocular tube U-TR30H-2+IX-ATU 3 step optical path selectable (observation: straight port = 100:0, 20:80, 0:100), interpupillary distance adjustable 50-76 mm,
diopter adjustment function, F.N. 22
Stage Cross stage with flexible right handle IX2-SFR 50 mm(X) X 50 mm(Y) stroke, stage insert plate exchangeable (ø110 mm)
Cross stage with short left handle IX-SVL2 50 mm(X) X 43 mm(Y) stroke, stage insert plate exchangeable (ø110 mm)
Plain stage IX2-SP 232 mm(X) X 240 mm(Y) stage size, stage insert plate exchangeable (ø110 mm)
Narrow plain stage IX2-KSP 160 mm(X) X 240 mm(Y) stage size, stage insert plate exchangeable (ø110 mm)
CK40-MVR Mechanical stage to be used with IX2-KSP, 120 mm (X) X 78 mm (Y) stroke
Gliding stage IX2-GS Upper circular stage 360° rotatable, 20 mm(X/Y) travel
Condenser Motorized long working IX2-LWUCDA2 Motorized turret with 6 position slots for optical devices (3 positions each for ø30mm and ø38 mm),
distance universal aperture iris diaphragm adjustable, N.A. 0.55 / W.D. 27 mm
Long working distance Relief Contrast IX2-MLWCD 4 positions for optical devices (for ø50 mm, Relief Contrast optical devices rotatable), aperture iris diaphragm adjustable,
N.A. 0.5 / W.D. 45 mm
Eyepiece WHN10X High eyepoint, F.N. 22
WHN10X-H High eyepoint, diopter adjustment function, F.N. 22
Reflected light Fluorescence illuminator IX2-RFAL L-shaped design with exchangeable F.S. and A.S. modules, two filter holder sliders (2 positions, ø32 mm, t=6 mm or less)
fluorescence unit IX2-RFA Straight design with field iris diaphragm, filter holder slider (2 positions, ø32 mm, t=6 mm or less)
Fluorescence cube turret IX2-RFACA Motorized turret with 6 positions, built-in shutter
Light source 100 W Hg apo lamp housing and transformer, 100 W Hg lamp housing and transformer or
75 W Xe lamp housing and transformer
IX81 specifications
Focusing position Dry objective Interface between air and cover glass
Oil immersion objective Interface between sample (cultured liquid) and cover glass
Offset method Controlled by software Compensation for shift of observation position toward the focusing plane is by Z-axis control (built into the IX81-ZDC)
Observation methods Fluorescence /DIC: DIC cannot be used beside gray-sensitive colors.
Dichromatic mirror IN/OUT method for AF laser introduction Manual exchange
F.N. limitation Light volume is low at the image perimeter for F.N. 22 when using 2X, 4X, 10X objectives
Focusing speed Within approx. 0.8 seconds (average) from near focusing position) Speed also varies according to the start position of auto focusing, and
(not including offset time through software individual PC performance
Focusing accuracy ±0.3 µm (when environmental temperature change is within 5°C)
Laser safety standard Class 1 (JISC6802, IEC825, CDRH)
Laser safety function Front monitor method (Laser light volume by special PD) IEC60825
Camera port Left side port Can only be combined with U-TV1X-2+U-CMAD3, U-DPCAD, U-SIP (primary image , 1X)
Observation tube IX-ATU+U-TR30H-2+IX-TVAD+U-CMT
IX-ATU+U-TR30-2+U-TV1X-2+U-CMAD3
IX81-ZDC specifications

3029
IX71/IX81 SYSTEM DIAGRAM
1
2
A B C D
4
Stages Illumination pillars, condensers
Fluorescence
illuminators
Observation tubes,
eyepieces
3
1
2
A B C D Camera adapters, camera ports E
4
Stages Illumination pillars, condensers
Fluorescence
illuminators
Observation tubes,
eyepieces
3
A
C
E
DD
D
E
F
D
C
B D
A B
2
1
G
AAF
G
G3
3
3
4
44
5
22
11
5
H
H
E
CO2
IX-ATU
Intermediate tube
U-BI90
Binocular tube
U-BI90CT
Binocular tube with
centering telescope
GX-SPU
Side port
intermediate tube
U-TVZ
Zoom camera
port tube
U-TV1X-2
Primary image
camera port tube
U-TV0.5XC-3
C-mount camera
port tube with
0.5x lens
U-TV0.63XC
C-mount camera
port tube with
0.63x lens
U-TV0.35XC-2
C-mount camera
port tube with
0.35x lens
U-TV0.25XC
C-mount camera
port tube with
0.25x lens
U-CMAD3
C-mount
adapter
U-BMAD
Bayonet mount
adapter
U-FMT
F mount
adapter
U-CMT
C-mount
adapter
IX-TVAD
Primary image
camera port tube
U-TMAD
T mount
adapter
U-SMAD
Sony mount
adapter
U-FWO■
Motorized
observation
filter wheel
U-TR30H-2
Trinocular tube U-TR30-2
Trinocular tube
U-ECA
Magnification
changer 2x
U-ECA1.6x
Magnification
changer 1.6x
U-CA
Magnification changer
1x, 1.25x, 1.6x, 2x
U-EPA2
Eyepoint adjuster
U-KPA
Intermediate attachment for
simple polarising observation
IX2-PRLBP1-2 (100%) ▲●
100% prism unit for lower back port
IX2-PRLBP8-2 (20%/80%) ▲●
20/80% prism unit for lower back port
IX2-MDICT
Analyzer unit for
transmitted light
Mirror unts
U-TBI90
Tilting binocular tube
U-DPCAD
Dual port
tube with
C-mounts
IX2-RFACA■
Motorized
fluorescent cube
IX2-RFAC
Mirror unit turret
IX2-CA2X-2 ▲
Intermediate magnification
lens 2X unit
IX2-TVRAC ■▲
Motorized bottom port
unit with C-mount
IX2-LBPC ▲●
Lower back port
unit with C-mount unit
IX2-TVR ▲
Bottom port unit
IX-HOP
Petri dish
holder
IX-HOT
Terasaki
plate holder
IX-HOS
Slide glass
holder
IX-PP24NUN
Scale for
NUNCTM plate
IX-PPM
Millimeter
scale
IX-CLMT,
IX-CLM96
Well positioner
WHN10X
WHN10X-H
Eyepieces
IX-SVL2
Cross stage with
short left handle
IX2-SFR
Cross stage with
flexible right handle
IX2-SP
Plain stage IX2-GS
Gliding stage
IX2-NPS
Nosepiece stage
IX2-RSPC-2 ◆▲
Right side port
attachment with C-mount
IX2-GCP
Glass stage
insert plate
U-FMP
Mechanical stage
MIU-IBC-I, MIU-IBC-IF
CO2incubator
IX-CP50
Insert plate
IX-SCL
Slide clip
Thermoplate
CK40-MVR
Mechanical stage
IX-MVR
Mechanical stage
IX-SUSP
Stage plate
Incubator
IX2-KSP
Narrow plain stage
CK2-SS
Side stage
U-SIP*
Split primary image
camera port
Cube cassette
for full image/
split images*
U-IFFH
Focus handle
Interface
U-ZPCB2
Z motor control
board
RS232C calble
U-FH
Focus handle
PC-AT
(Windows XP/Vista)
U-HSTR2
Handset
U-DULHA
Double lamp
housing adapter
U-LH100-3
100 W halogen
lamp housing
U-LH100L-3
100 W halogen lamp housing
U-RMT
Extension cord
U-DICTS
Shift DIC slider for
transmitted light
U-DICTHR
High resolution DIC slider for
transmitted light
U-DICTHC
High contrast DIC slider
for transmitted light
IX2-ILL100
Transmitted
illuminator
45LBDIF
45ND25
45ND6
43IF550W45
Filter
IX-ULWCD
Ultra long working distance condenser
IX-ADUCD
Condenser adapter
U-FWR■
Motorized
reflected
filter wheel
U-UCD8-2
Universal condenser
U-TLO
Oil top lens
U-TLD
Dry top lens
U-TLO
Oil top lens
U-TLD
Dry top lens
IX2-MLWPO
Polarizer
attachment
IX2-MLWCD
Mid long
working distance condenser
IX2-DICD
Water immersion DIC condenser
IX2-TLW
Water top lens IX2-RFAL
L-shaped fluorescence
illuminator
IX2-RFA
Fluorescence
illuminator
32ND6
32ND12
32ND25
32ND50
Filters
IX2-FP
Frame plate
adapter
IX2-AN
Analyzer
IX2-AN
Analyzer
IX2-UCB2
System controller
Optical
elements
Optical
elements
Optical
elements
Optical
elements
IX-LWPO
Polarizer
attachment IX2-LWUCD
Long working distance
universal condenser
IX71S1F-3/
IX71S8F-3
IX71 frame with
100% prism/
IX71 frame with
20/80% prism
IX81F-3 ■▲
IX81 frame
without prism
IX81S1F-3 ■▲
IX81 frame
with 100% prism
IX81-ZDC ■▲
IX81-ZDC frame
with 100% prism
IX2-SHA■
Motorized
shutter
IX2-LWUCDA2■
Motorized long
working distance
universal condenser
TH4-HS
Hand switch TH4-100
Power supply
unit
IX2-IFSHA■
Shutter interface
U-ANT
Analyzer for
transmitted light
U-DICT
DIC slider for
transmitted light
IX2-DSU*
Disk scanning unit
U-RFSS
Rectangular field
stop
IX-RFSPOT
Pinhole field stop
IX2-RFRS
Ring slit
illumination unit
IX2-RS40,
IX2-RS60,
IX2-RS100
Ring slit illumination unit
IX2-RFAW *
Double lamphouse
illuminator
* Not avilable in some areas
■Operation via IX2-UCB2
▲To consult with local Olympus subsidiary
◆Made to order product
▼Not recommended for IX2-DSU combination
●Choose either prism unit for IX2-LBPC
Illustrations colored cyan shows
motorized units.
U-CLA
Collector lens adjustment
U-LH75XEAPO
75 W xenon apo
lamp housing
U-RFL-T
Power supply unit
for mercury lamp
U-RX-T
Power supply unit
for xenon lamp
U-LH100HG
100 W mercury
lamp housing
U-LH100HGAPO
100 W mercury apo
lamp housing
B
U-TV1xC
C mount
camera
adapter