VWR VisiScope 534 Series User manual

Instruction manual
VWR®Microscope VisiScope
Series 534
VERSION: 1.0
ISSUED: 01/02/2021
Model European Catalogue Number
TL534B FL BG 630-3270
TL534B FL4 630-3243
TL534B-SA FL4 630-3244
VisiCube TL_B 630-3245
VisiCube TL_G 630-3246
VisiCube TL_V 630-3247
VisiCube TL_UV 630-3248
VisiCube TL_R1 630-3249
VisiCube TL_R2 630-3250
VisiCube TL_R3 630-3251
VisiCube TL_FR 630-3252
VisiCube TL_A 630-3253

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MANUFACTURED FOR:
Europe
VWR International bv
Researchpark Haasrode 2020
Geldenaaksebaan 464
B-3001 Leuven
+ 32 16 385011
be.vwr.com
UK Importer
VWR International Ltd
Hunter Boulevard, Magna Park
Lutterworth, Leicestershire, LE17 4XN
uk.vwr.com
Country of Origin
Italy

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Table of contents
1. Warning 3
2. Safety Information 3
3. Intended use 3
4. Symbols and conventions 3
5. Instrument description 4
6. Unpacking 6
7. Assembling 6
7.1 Microscope assembling 7
7.1.1 Installing fluorescence filter 8
7.1.2 Replacing fluorescence filter 9
7.2 Polarizing set (optional) 10
8. Brightfield observation procedures (transmitted light) 11
9. Use of the microscope (transmitted light) 12
9.1 Light intensity adjustment 12
9.2 Coarse focus tension adjustment 12
9.3 Coarse upper limit lever 12
9.4 Stage 12
9.5 Diopter adjustment 13
9.6 Adjusting the interpupillary distance 13
9.7 Light path selection 13
9.8 Use of eye shields 13
9.9 Centering the condenser 14
9.10Effects of the field diaphragm 14
9.11 Aperture diaphragm 14
9.12 Use of oil immersion objective 15
9.13Use of the polarizer (optional) 15
10. Fluorescence observation procedures (reflected light) 16
11. Use of the microscope (reflected light) 17
11.1 LED switch on 17
11.2 Use of fluorescence 17
11.3 Use of light excluding plate 18
11.4 Use of UV shield 18
12. Microphotography 19
12.1 Use of C-mount cameras 19
12.2 Use of reflex cameras 19
13. Troubleshooting 20
14. Repair and maintenance 22
15. Technical service 22
16. Warranty 22
17. Compliance with local laws and regulations 22
Disposal 23

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1. Warning
This microscope is a scientific precision instrument designed to last for many years with a minimum
of maintenance. It is built to high optical and mechanical standards and to withstand daily use.
We remind you that this manual contains important information on safety and maintenance, and
that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use that does not comply with
this manual.
2. Safety information
Avoiding electrical shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in off position.
Users should observe all safety regulations of the region. The equipment has acquired the CE
safety label. However, users have full responsibility to use this equipment safely.
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of
the unit.
3. Intended use
For research and teaching and teaching use only. Not intended for any animal or human
therapeutic or diagnostic use.
4. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

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5. Instrument description
PHOTO/TV PORT
DIOPTER
ADJUSTMENT
RING
NOSE PIECE
MAIN SWITCH /
TRANSMITTED LIGHT
ADJUSTMENT KNOB
OBSERVATION
HEAD
CONDENSER
HEIGHT ADJUSTMENT
KNOB
EYEPIECES
CONDENSER
CONDENSER
CENTERING
SCREWS
SLIDE HOLDER
COARSE
FOCUS
KNOB
COARSE
UPPER
LIMIT KNOB
FINE FOCUS
KNOB

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Opposite side
APERTURE
DIAPHRAGM
X/Y MOVEMENT
KNOBS
OBJECTIVES
FIELD
DIAPHRAGM
STAGE
LIGHT PATH
SELECTOR LEVER
FLUORESCENCE
CUBE SELECTOR
LEVER
UV SHIELD
REFLECTED LIGHT
ADJUSTMENT KNOB
TENSION
ADJUSTMENT
RING

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6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of
the container and lift the top half of the container. Take some care to avoid that the optical items
(objectives and eyepieces) fall out and get damaged. Using both hands (one around the arm and
one around the base), lift the microscope from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, filters or glasses.
Traces of grease or other residuals may deteriorate the final image quality and
corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
1 Microscope frame
2 Eyepieces
3 Objectives
‒TL534B FL BG / TL534B FL: W-PLAN series
‒TL534B-SA FL4: W-PLAN F series
4 Observation head
5 Epi-illuminator
6 Allen wrench
1
3
2
87
6
10
4
9
12
11
5
7 Tension adjustment tool
8 Immersion oil
9 Dust cover
10 Power supply
‒6V for transmitted light
‒12V for fluorescence
11 Light excluding plate
12 UV protection shield

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7.1 Microscope assembling
1 Insert the round dovetail socket of the illuminator (1) into the
hole in the microscope body and tighten the locking screw(2).
(Fig 1).
2 Insert the power supply jack in the connector (3) placed at the
rear side of the illuminator. (Fig. 2)
3 Insert the optical head above the epi-illuminator and tighten
the screw (4). (Fig. 3)
Hold the head with one hand during the locking in order to
avoid that the head falls.
4 Insert both eyepieces into the tubes of the optical head.
(Fig.4)
Condenser is pre-installed in the factory. To remove the
condenser use an Allen wrench 1,5 mm diam and operate on
the locking screw placed on the right side of the condenser
holder.
12
FIGURE 1
3
FIGURE 2
4
FIGURE 3
FIGURE 4

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5 Screw each objective into the thread of the nosepiece,
clockwise with increasing magnification. (Fig. 5)
6 Insert the power supply jack in the connector placed at the
rear side of the microscope. (Fig. 6)
7.1.1 Installing fluorescence filter
This procedure is only necessary for TL534B FL and
TL534B-SA FL4 models. TL534B FL BG model comes with B and
G cubes preinstalled.
1 Disconnect the power supply plug from the fluorescence
illuminator.
2 Open the side cover of the illuminator, by unscrewing the side
screws (1). (Fig. 7)
It could be helpful to remove the observation head.
The cubes are mounted on the opposite side of the cover:
opening the left cover acts on the right side of the slider and
vice versa.
3 Open the top door of the fluorescence illuminator by
unscrewing the four screws (2) and release the cover. (Fig. 8)
FIGURE 5
FIGURE 6
1
FIGURE 7
2
FIGURE 8

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4 Loosen the front locking screw (3) on the fluorescence cube
slider. (Fig. 9)
5 Insert the fluorescence cube into the dovetail (4) of the cube
slider and move it to the click position. (Fig. 10)
6 Insert the cube connection cable into the illuminator.
7 Tighten the locking screw (3). (Fig. 10)
8 Connect the plug of the fluorescence cube (5) in one of the
free connectors (6) to power the LED. (Fig. 11)
9 Apply the adhesive marker (3) for the fluorescence cube on
the illuminator. (Fig. 12)
10 Close the top door.
11 Close the side cover.
12 Connect the power supply.
13 Start working.
7.1.2 Replacing fluorescence filter
1 Disconnect the power supply plug from the fluorescence
illuminator.
2 Open the side cover of the illuminator, by unscrewing the side
screws (1). (Fig. 7)
It could be helpful to remove the observation head.
The cubes are mounted on the opposite side of the cover:
opening the left cover acts on the right side of the slide and
vice versa.
3 Open the top door of the fluorescence illuminator by
unscrewing the four screws (2) and release the cover. (Fig. 8)
4 Loosen the front locking screw (3) on the fluorescence cube
slider. (Fig. 9)
5 Disconnect the plug (5) related to the cube you want to
replace. (Fig. 11)
6 Remove the fluorescence cube from the dovetail of the cube
slider.
7 Repeat steps 4. to 9. of paragraph 7.1.1. to install a new
fluorescence cube.
FIGURE 9
3
FIGURE 12
7
FIGURE 10
4
FIGURE 11
6
5

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7.2 Polarizing set (optional)
1 Place the polarizer on the light exit (1) at the base of the
microscope. (Fig. 13)
2 Loosen the head fixing knob (2) and remove the head from
the microscope frame. (Fig. 14)
3 Insert the analyzer into the hole inside the frame (3). (Fig. 15)
4 Put back the head into its original position and lock the fixing
knob.
FIGURE 14
2
FIGURE 15
3
FIGURE 13
1

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(Used commands) (Chapter)
Set the main swith to “ON” and adjust light intensity.
Place a slide on the stage.
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the spec-
imen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
8. Brightfield observation procedures (transmitted light)
Main switch / brightness
adjustment knob
Nosepiece
Observation head
Diopter adjustment ring
Condenser centering
Aperture diaphragm
Field diaphragm
Nosepiece
Light adjustment dial
Coarse and fine focusing knobs
Coarse and fine focusing knobs
9
5
5
5
5
9
9
9
9
9
9
Slide holder 9

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9. Use of the microscope (transmitted light)
9.1 Light intensity adjustment
Operate on the light intensity adjustment knob to turn ON/OFF
the microscope and to increase/decrease the illumination
voltage (1). (Fig. 16)
9.2 Coarse focus tension adjustment
Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
To modify the tension according to personal’s needs, rotate the
ring (2) using the provided tool. (Fig. 17)
Clockwise rotation increases the tension.
If the tension is too loose, the stage could go lower by itself or
the focus easily lost after fine adjustment. In this case, rotate the
knob in order to increase the tension.
9.3 Coarse upper limit lever
The upper limit knob has two functions: prevent the contact
between slide and objective and acts as “focus memory”.
1 After focussing the specimen, pull the lever (3) toward the
front of the microscope and lock it. (Fig. 18).
In this way the focus upper limit is set.
2 Now one can lower the stage with coarse focus knob, replace
the specimen and raise again the stage up to the upper limit:
specimen will be in approximate focus and will need a fine
adjustment to get the proper focus.
‒Fine focus movement is not affected by the coarse focus lock.
‒To unlock, move the lever in the opposite direction to the one
used for the locking.
9.4 Stage
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverlside 0,17 mm.
It is possible to place two slides side by side on the stage.
Open the spring arm of the slide holder (4) and place frontally
the slides on the stage. (Fig. 19)
Gently release the spring arm of the slide holder.
A sudden release of the spring arm could cause the falling of
the slide.
1
FIGURE 16
2
FIGURE 17
Lock
Unlock
3
FIGURE 18
4
FIGURE 19

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9.5 Diopter adjustment
1 Look into the right eyepiece with your right eye only, and
focus on the specimen.
2 Look into the left eyepiece with your left eye only. If the
image is not sharp, use the diopter adjustment ring (1) to
compensate. (Fig. 20)
The adjustment range is ±5 diopter. The number indicated
on the adjustment ring graduation should correspond to the
operator’s dioptric correction.
9.6 Adjusting the interpupillary distance
Observing with both eyes, hold the two eyepiece prism
assemblies. Rotate them around their common axis until the
fields of view coincide.
The graduation on the interpupillary distance indicator (2),
pointed by the spot “.” on the eyepiece holder, shows the
distance between the operator’s eyes. (Fig. 21)
The range of the interpupillary distance is 48-75 mm.
9.7 Light path selection
The observation head is equipped with an optical path selector
that allows the light to be split to the eyepieces and to the
photo/TV port.
1 Move the selector (3) to one of the two possible positions to
split the light. (Fig. 22)
Position Light
In 100% Eyepieces
Out 100% Tv
9.8 Use of eye shields
Use with eyeglasses
Fold rubber eyeshields with both hands. Folded eyeshields avoid
scratching the lenses of eyeglasses. (Fig. 23)
1
FIGURE 20
2
FIGURE 21
3
FIGURE 22
FIGURE 23

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Use without eyeglasses
Raise eye shields and observe at the microscope placing
eyes to the shields, avoiding external light to disturb the
observation. (Fig. 24)
9.9 Centering the condenser
1 Place the specimen on the stage, insert 10x objective into the
light path and focus.
2 Insert the front lens of the swing-out condenser (1). (Fig. 25)
3 Rotate the field diaphragm ring (2) in counterclockwise
direction, to fully close the diaphragm.
4 Rotate the condenser height adjustment knob (3) to focus the
edges of the diaphragm.
5 Rotate the two centering screws (4) to bring the bright spot in
the center of the field of view.
6 Gradually open the diaphragm. The condenser is centered
when the diaphragm image is symmetrical to the field of
view.
7 In normal use, open the diaphragm until it circumscribes the
field of view.
9.10 Effects of the field diaphragm
Field diaphragm adjusts the illuminated area to obtain a high
contrast image.
Set the diaphragm according to the objective in use until it
circumscribes the field of view, in order to eliminate unnecessary
light to eyepieces. (Fig. 26)
9.11 Aperture diaphragm
The Numerical Aperture (N.A.) value of the aperture diaphragm
affects the image contrast. Increasing or reducing this value
one can vary resolution, contrast and depth of focus of the
image.
With low contrast specimens set the numerical aperture value
(1) (printed on the condenser ring) to about 70%-80% of the
objective’s N.A. (Fig. 27) If necessary, remove on eyepiece and,
looking into empty sleeve, adjust the condenser’s ring in order
to obtain an image like the one in fig. 28.
Example: with objective PLAN 40x/0,65 set the scale to
0.65 x 0.8 = 0,52
FIGURE 24
1
3
2
4
FIGURE 25
FIGURE 26
1
FIGURE 27

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9.12 Use of oil immersion objective
1 Focus the specimen with a low power objective.
2 Lower the stage (remembering to lock the coarse upper limit
lever).
3 Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 29)
Make sure that there are no oil bubbles. Air bubbles in the oil
damage the image quality.
To check for bubbles: remove an eyepiece, fully open the
aperture diaphragm and observe the objective exit pupil. (The
pupil must be round and bright).
To remove the bubbles, gently move the nosepiece to the right
and left to move the immersion objective a few times and allow
the air bubbles to move.
4 Insert immersion objective.
5 Return the stage to the upper focusing point and obtain an
optimal focus using the fine focus knob.
6 After use, gently remove the oil with a soft paper towel or a
lightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute ethyl alcohol (30%).
The immersion oil, if not immediately cleaned, could crystallize
creating a glass-like layer. In this situation the observation of
the specimen would be difficult if not impossible due to the
presence of an additional thickness on the objective.
9.13 Use of the polarizer (optional)
1 Remove the specimen from the stage.
2 Looking inside the eyepieces, rotate the polarizer until the
darkest position is achieved.
3 Once the dark is achieved (“extinction” or “Crossed Nicol”
position) it is possible to begin the observation.
FIGURE 29
FIGURE 28
Aperture
Diaphragm
Field of view
30-20%
70-80%

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10. Fluorescence observation procedures (reflected light
Fluorescence main switch
(Used commands) (Chapter)
Slide holder
Fluorescence filter turret
Nosepiece
Fluorescence main switch
Coarse and fine focusing knobs
Turn the fluorescence main switch to turn on the LED
Put a specimen on the stage
Insert in the light path the filter suitable for the specimen
Begin observation
Insert desired objective for the observation and
focus the specimen
Adjust light intensity
11
9
11
5
5
11

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11. Use of the microscope (reflected light)
11.1 LED switch on
Turn the main switch (1). (Fig. 30)
Adjust to the desired brightness by rotating the dial (1).
11.2 Use of fluorescence
The filter turret is provided with 4 positions.
In each of the four positions a fluorescence filter can be
inserted, which can be selected from the options shown in the
table below.
You can always add or replace an additional filter after the
first installation (see section 7.1.1 and 7.1.2).
If all four turret positions are full, the observation in
transmitted light will be affected by the presence of the
fluorescence filter.
1 Move the filter selector (2) in the desired position. (Fig. 31)
2 When the filter is in the click stop position, the dedicated
LED will turn on.
When switching the fluorescence filter, the LED light goesout.
This is not a defect.
1
2
Filter name Excitation filter Dichroic mirror Barrier filter Applications
VisiCube TL_UV 325-375 nm 415 nm 435LP nm ‒DAPI
‒Hoechst
VisiCube TL_V 390-420 nm 440 nm 450LP nm ‒Coumarin
‒Pacific Blue
VisiCube TL_B 455-495 nm 500 nm 510LP nm ‒FITC
‒GFP
VisiCube TL_G 510-550 nm 570 nm 575LP nm
‒Rhodamine
‒TRITC
‒Propidium Iodide
VisiCube TL_r1 582-603 nm 610 nm 615-645 nm ‒Red#2 FISH
VisiCube TL_r2 590-650 nm 660 nm 665LP nm ‒Cy5
VisiCube TL_r3 595-645 nm 655 nm 665-715 nm
‒Cy5
‒AlexaFluor 647
‒Draq5
VisiCube TL_fr 623-678 nm 685 nm 690-750 nm ‒Cy5.5
VisiCube TL_a 720-760 nm 770 nm 780LP nm ‒Li_Cor IR dye 800
FIGURE 31
FIGURE 30

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11.3 Use of light excluding plate
Microscope is provided with a light excluding plate that can be
placed on the stage and prevents flare and reflections coming
from the condenser front lens.
The plate can be used in two different ways.
Mode n° 1: place the plate on the stage (under the slide holder)
and place the slide directly over the plate. (Fig. 32)
Mode n° 2: lower the condenser and insert the plate between
the two layers of the stage. (Fig. 33).
In both cases it is possible to move the sample using the stage
X-Y translation knobs.
11.4 Use of UV shield
Microscope is provided with a UV protection shield. This can
be used to protect user from unwanted UV rays coming from
the fluorescence light source.
1 Loosen the two locking screws (1). (Fig. 34)
2 Insert the grooves of the UV shield (2) into the holes (Fig. 35)
and tighten the screws (1) again.
1
2
FIGURE 32
FIGURE 33
FIGURE 34
FIGURE 35

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12. Microphotography
12.1 Use of C-mount cameras
1 Loosen the clamping screw (1) on the trinocular port and
remove the dust cap (2). (Fig. 36)
2 Screw the C-mount adapter (3) to the camera (4) and insert
the round dovetail of the C-mount into the empty hole of the
trinocular port, then tighten the clamping screw (1). (Fig. 37)
12.2 Use of reflex cameras
1 Insert the Reflex adapter (1) into the relay tube to the
microscope (2).
2 Screw the “T2” ring (3) (not provided) to the reflex adapter.
3 Connect the Reflex camera (4) to the “T2” just installed
(Fig.38).
4 Mount the other end of the relay tube (2) into the empty
hole of the trinocular port, then tighten the clamping screw.
(Fig.36)
“T2” ring is not provided with the microscope, but is
commercially available.
While shooting dark specimens, darken eyepieces and
viewfinder with a dark cloth to minimize the diffused light.
To calculate the magnification of the camera: objective
magnification * camera magnification
* lens magnification.
If using an SLR camera, mirror movement may cause the
camera to vibrate.
We suggest lifting the mirror, using long exposure times and a
remote cord.
3
4
1
3
2
4
1
2
FIGURE 36
FIGURE 37
FIGURE 38