Warner Instruments SHM Series User manual

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In ns st tr ru uc ct ti io on ns s f fo or r t th he e S SH HM M S Se er ri ie es s
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Mu ul lt ti i- -l li in ne e S So ol lu ut ti io on
n H He ea at te er rs s
General
In-line solution heating is a simple and effective
method of providing warmed perfusion solutions to a
bath.
The removable, minimal dead space manifold at the
heater output allows the SHM Series Multi-line
Solution Heaters to be used in applications where
from 2 to 8 perfusion lines are connected to a
chamber or other device.
SHM Series Solution Heaters are designed for use
with the Warner TC-324 and TC-344 Heater
Controllers (all models). The compact design of the
heater makes it possible to install the device
immediately adjacent to the input port of a perfusion
chamber insuring minimum heat loss. Heaters can
accommodate flow rates up to 5 ml/min.
Each heater is supplied with a TA-29 thermistor cable
assembly (which allows for monitoring of the actual
bath temperature; T2 output on the heater controller),
a 3-way valve, rubber o-rings and 3 meters of PE-160
tubing.
Tubing Connections
PE-160 tubing (or any other tubing with a 1/16” (1.5
mm) OD is used to connect SHM Series heaters to
both the solution reservoir and the perfusion
chamber.
Connections are made by press fitting the tubing onto
the 18 gauge stainless steel hypodermic tubes. Cap
plugs are supplied to block unused inputs.
Assembly
1. Attach your solution reservoirs to the input ports
of the inline heater using PE-160 or equivalent
tubing.
2. All flow lines within the heater must be fluid-
filled prior to use. A 3-way valve is supplied
to facilitate filling of these lines.
Using a short length of PE-160 tubing, attach
the 3-way valve to the output end of the heater.
Attach a vacuum line to the side port of the
stopcock.
3. Sequentially fill all input ports on the device with
fluid.
Begin by filling unused input ports with distilled water.
Block each port using the supplied plugs.
Next fill each solution input port with perfusion solution.
4. Remove the 3-way valve and connect the output port of
the heater to a perfusion chamber using PE-160 tubing
or equivalent.
5. Place the outboard tubing from the chamber into a
collection reservoir such as a paper cup.
Use a short length of PE tubing between the heater
output and perfusion chamber to minimize heat loss
during delivery.
6. Connect the power cable from the SHM Series heater
to the output terminal of your heater controller.
If using a Warner Heater Controller, connect the TC-29
thermistor cable assembly to the rear panel input of the
heater channel being used on the controller.
Operation
1. Start the solution flow and set the flow rate to the
desired level.
Typical flow rates are 5 ml/min or less and the heater
can sustain temperatures up to 50°C under these
conditions. Faster flow rates are supported but the
output temperature will not be maintained.
Warner Instruments, Inc.
1125 Dixwell Avenue, Hamden, CT 06514 USA •[phone] •[phone] •Fax [phone]

Rev 01.19.04
2. Turn the power to the heater on and adjust the
heater controller to the desired temperature.
Allow a few minutes for the system to stabilize.
3. If using a Warner heater controller, place the
TA-29 thermistor in the output flow path near
the sample. Read the working solution
temperature by selecting T2 on the controller.
Adjust the SET TEMP on the controller to
compensate for heat loss from the heater to the
bath.
4. Additional adjustment of the heater voltage may
be required if any of the following change
substantially during the experiment. Efforts to
minimize these factors will be rewarded.
• Solution flow rate
• Temperature of solution entering the
heater
• Ambient (room) temperature
• Air currents around chamber
Cleaning
The SHM output manifold is removable to facilitate
cleaning of the output path. (See image to the right.) Gently
unscrew the retaining cap to reveal the manifold assembly.
Replacement rubber o-rings (2) are supplied to extend the
life of the instrument.
Maintenance
Salt solutions can be corrosive to metal components and
can shorten the life of the heater if left in the unit during
storage.
The heater should be flushed with distilled water and
blown dry after each use to eliminate the effects of salt
and moisture.
Outgassing
A common problem with rapid heating of solutions is
outgassing. The bubbles formed can often cause blockages
or disruptions to the flow in the chamber bath. An effective
solution to this problem is to pre-warm the perfusate at the
reservoir. Warner Instruments carries a full line of Solution
Reservoir Heaters designed to work in concert with the SHM
Series In-Line Solution Heaters.
Specifications
Heater resistance 10 Ω
Voltage requirement variable to 12 V
maximum
Maximum temperature 50 °C
Manifold dead volume 30 µl
Maximum flow rate 5 ml/min *
* See table next page
Physical Dimensions
Body 16.5 mm diameter x 16.5 cm long
Weight 104 g
Cable length 1.9 m
Warranty 1 year
Accessories
TA-29 Replacement cable with bead thermistor
TA-30 Replacement cable with glass thermistor
PE-160/10 Polyethylene tubing 3 meter
MB/B Magnetic base
U9404 3-Prong clamp
Actual temperature at device outflow port as a function of flow rate.
Warner Instruments, Inc.
1125 Dixwell Avenue, Hamden, CT 06514 USA •[phone] •[phone] •Fax [phone]

Rev 01.19.04
Set temp = 35 °C
Flow rate (ml/min) Monitored temp (°C ) Output voltage (V)
1 34.0 4.44
2 33.4 5.20
3 32.5 5.72
4 31.7 6.10
5 31.0 6.86
Set temp = 37 °C
Flow rate (ml/min) Monitored temp (°C ) Output voltages (V)
1 35.9 4.85
2 35.6 5.53
3 34.6 6.31
4 34.1 7.03
5 33.2 7.46
Set temp = 40 °C
Flow Rate (ml/min) Monitored Temp (°C ) Output voltages (V)
1 38.9 5.22
2 38.2 6.13
3 37.3 6.87
4 36.3 7.45
5 36.7 7.52
Set temp = 45 °C
Flow Rate (ml/min) Monitored Temp (°C ) Output voltages (V)
1 43.2 6.15
2 42.4 6.88
3 41.9 7.72
4 41.4 8.37
5 40.9 8.75
Set temp = 50 °C
Flow Rate (ml/min) Monitored Temp (°C ) Output voltages (V)
1 47.9 6.89
2 47.4 7.85
3 47.2 8.95
4 46.2 9.69
5 44.6 10.3
Warner Instruments, Inc.
1125 Dixwell Avenue, Hamden, CT 06514 USA •[phone] •[phone] •Fax [phone]